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[molecular identification and the features of genetic diversity in interspecific hybrids of amur sturgeon (acipenser schrenckii x a. baerii, a. baerii x a. schrenckii, a. schrenckii x a. ruthenus, and a. ruthenus x a. schrenckii) based on variability of multilocus rapd markers].the method of polymerase chain reaction with random primers (rapd pcr) was used to identify the progeny of the crosses between three sturgeon species, amur sturgeon (acipenser schrenckii brandt, 1869), siberian sturgeon (a. baerii brandt, 1869), and sterlet (a. ruthenus linnaeus, 1758). using ten primers, genetic variation in 70 yearlings, produced in seven individual crosses: acipenser schrenckii x a. schrenckii, a. baerii x a. baerii, a. ruthenus x a. ruthenus, a. schrenckii x a. baerii, a. ba ...200819137727
tgf-beta3 exists in bony fish.a partial nucleotide sequence of transforming growth factor-beta3 (tgf-beta3) has been isolated from the siberian sturgeon (acipenser baeri), rainbow trout (oncorhynchus mykiss) and european eel (anguilla anguilla), confirming a ubiquitous presence in the ray-finned (actinopterygian) bony fish. the bony fish tgf-beta3 is highly conserved, with some 83-84% nucleotide identity (coding region) and 90-95% predicted amino acid identity to known homeotherm tgf-beta3's. far lower homologies are apparen ...199910614492
characterization of beta2-microglobulin in a primitive fish, the siberian sturgeon (acipenser baeri). 199910541811
ig heavy chain of the sturgeon acipenser baeri: cdna sequence and diversity.to investigate the gene organization of the igh locus, and the vh diversity of the siberian sturgeon, a cdna library was constructed and screened with vh-specific probes from two holostean fish. isolated clones were analyzed and domain-specific probes used in rescreening of the library, southern blot analysis, and northern blots. it was concluded that the siberian sturgeon has one igh locus with a translocon type of organization. two allelic variants of the mu gene were found, with identities ra ...20069799333
ascorbic acid status as affected by dietary treatment in the siberian sturgeon (acipenser baeri brandt): tissue concentration, mobilisation and l-gulonolactone oxidase activity.a study was conducted to evaluate tissue storage and mobilisation of l-ascorbic acid (aa) in the siberian sturgeon (acipenser baeri) fed three different experimental diets. the three treatments consisted of a diet devoid of vitamin c (diet a0) and two diets supplemented with equivalent of 300 mg aa kg(-1) in the form of either silicone-coated ascorbic acid (diet sc) or of ascorbyl-2-polyphosphate (diet ap). during the first phase (4 months) of the trial, six batches of 130 siberian sturgeon (ini ...199624194303
ig light chain gene in the siberian sturgeon (acipenser baeri).elasmobranch and teleost fish have their ig light (l) chain loci organized in multiple clusters (vl-jl-cl). the vl segments of teleosts are in opposite transcriptional orientation to the cl genes, suggesting that in teleosts and elasmobranchs there may have been separate evolutionary events leading to this organization. to address this problem, the igl locus from the siberian sturgeon (acipenser baeri) (representative of a branch between elasmobranchs and teleosts) was investigated. sequence ana ...19968757324
diverse splicing pathways of the membrane ighm pre-mrna in a chondrostean, the siberian sturgeon.teleosts and tetrapods have evolved different splice patterns to generate their membrane-bound igm. in the tetrapod lineage, the first transmembrane exon is spliced to an internal cryptic site located close to the end of the fourth constant exon. because teleosts lack this site they use the regular 3'-splice site of the ch3 exon instead. we characterized the mum splicing patterns in a chondrostean, the siberian sturgeon. we observed a surprising diversity of splice patterns, the tm1 exon being s ...200919027787
circulatory and respiratory effects of an hypoxic stress in the siberian sturgeon.changes in circulatory, ventilatory and acid-base variables were studied in siberian sturgeon (acipenser baeri) exposed to acute and severe hypoxia (pwo2 = 10 torr), followed by a rapid return to normoxia. this treatment caused a significant stress, revealed by the high levels of plasma catecholamines and cortisol. the moderate circulatory changes firstly observed would represent the effects of increased plasma catecholamine levels together with an increased adrenergic nervous tone on the cardio ...19957481109
respiratory responses to progressive ambient hypoxia in the sturgeon, acipenser baeri.changes in respiratory and acid-base variables were studied in siberian sturgeon, acipenser baeri, during progressive deep hypoxia followed by recovery under normoxic conditions. during hypoxia, both ventilatory frequency and amplitude increased and this sturgeon was able to maintain standard oxygen consumption down to a low critical level of ambient po2 (pwo2 < 40 mmhg). during the posthypoxic period, an o2 debt was repaid by an elevated oxygen consumption (nearly double control value at 1 h), ...19938441872
long-term culture of a cell population from siberian sturgeon (acipenser baerii) head kidney.in vitro cultures of native fish cell lines are of great importance, both for basic research and applied science. in particular, there is strong demand for long-term growable cell lines from breeding fish, like sturgeon. here, we describe the culture of cells from siberian sturgeon (acipenser baerii) head kidney. the cells have so far been cultured over a period of 12 months (24 passages). cytochemical and immunocytochemical examination suggests that, in vitro, the cells exhibit markers that are ...200818958594
the estrogenic activity of certain phytoestrogens in the siberian sturgeon acipenser baeri.various phytoestrogens such as formononetin, daidzein, genistein and equol were synthesized. their purity was assessed by various analytical techniques including melting point determination, thin-layer chromatography (tlc), infra-red spectra (i.r. spectra), nuclear magnetic resonance (1h- and 13c-nmr) and gas chromatography coupled with mass spectrometry (gc-ms). the estrogenic activity of these compounds, as well as biochanin a and coumestrol, was biologically tested by the induction of vitello ...19912009221
spectral characteristics of photoreceptors and horizontal cells in the retina of the siberian sturgeon acipenser baeri brandt.in the retina of siberian sturgeon, three spectral classes of photoreceptors were identified by microspectrophotometry. these were rods, oil drop-containing and oil drop-free cones possessing p549, and oil drop-containing cones possessing p613 and p465. with intracellular recordings, rod-driven, cone-driven, and mixed horizontal cells of luminosity type were found, as well as color-opponent horizontal cells of, at least, 6 kinds. biphasic r/g cells received hyperpolarizing input either from rods ...19911771790
[protein composition of the blood serum of 2 allopatric populations of the siberian sturgeon acipenser baeri brandt]. 20035397549
[polymorphism of the mitochondrial dna control region in eight sturgeon species and development of a system for dna-based species identification].intraspecific and interspecific nucleotide sequence variations of the mtdna control region (d-loop) were studied with mtdnas isolated from tissue specimens of more than 1400 sturgeons of nine species: russian sturgeon acipenser gueldenstaedtii, persian sturgeon a. persicus, siberian sturgeon a. baerii, amur sturgeon a. schrenkii, fringebarbel sturgeon a. nudiventris, sterlet a. ruthenus, stellate sturgeon a. stellatus, beluga huso huso, and kaluga h. dauricus. the results were used to analyze th ...200818767539
the optimization of the protocol for immunofluorescence on fish spermatozoa.in comparison with mammals, the fertilization of fish occurs predominantly outside the organism in a water environment, where fish spermatozoa require specific conditions to interact with oocytes. it is evident that optimal conditions for fish and mammalian spermatozoa are quite different. this paper describes a special approach to handling fish (common carp and siberian sturgeon) spermatozoa in comparison with the samples originating from mammals (boar). this approach concerns not only the diff ...200818584860
the australian lungfish (neoceratodus forsteri) - fish or amphibian pattern of muscle development?the australian lungfish neoceratodus forsteri (dipnoi-sarcoterygians) is a likely candidate for the extant sister group of tetrapoda. transmission electron and light microscopy analysis revealed that the arrangement of somite cells of the lungfish resembles the structure of the urodelan somite. on the other hand, the pattern of early muscle formation in n. forsteri is similar to that found in the siberian sturgeon (acipenser baeri). during the early stages of myogenesis of n. forsteri, somite-de ...200818311718
the 'storm of the century' (december 1999) and the accidental escape of siberian sturgeons (acipenser baerii) into the gironde estuary (southwest france). an original approach for metal contamination.the gironde estuary is considered as a reference ecosystem with a large fish assemblage (75 species) and the presence of eleven diadromous species. however, geochemical studies and biomonitoring using oysters have shown high metal pollution levels. during the december 1999 hurricane, more than 5,000 young siberian sturgeons (acipenser baerii) accidentally escaped from a fish farm into the estuary, where this species had not been present until then.200818306893
[effect of photoperiod on growth, physiological and hematological indices of juvenile siberian sturgeon acipenser baerii].the effect of photoperiod on the growth of juvenile siberian sturgeon acipenser baerii has been studied in aquarium experiments. the maximum growth rate of juvenile siberian sturgeon was observed under 12, 16, and 24 h photoperiods. this was accompanied by high respiratory rate and total food consumption and conversion. rearing in the dark significantly decreased these indices. the hematological indices were within the normal range under optimal photoperiodic conditions. pronounced neutrophilia ...201619771629
evolutionary aspects of intestinal bicarbonate secretion in fish.experiments compared intestinal hco3- secretion in the intestine of marine teleost gulf toadfish, opsanus beta, to representatives of early chondrostean and chondrichthyan fishes, the siberian sturgeon, acipenser baerii, and white-spotted bamboo shark, chiloscyllium plagiosum, respectively. as seen in marine teleosts, luminal hco3- concentrations were 10-fold plasma levels in all species when exposed to hyperosmotic conditions. while intestinal water absorption left mg2+ and so4(2-) concentrated ...200616503178
stress and its relation to endocrine function in captive female siberian sturgeon (acipenser baeri).stress responses to numerous environmental conditions have been studied in a wide range of fish species. defining the relationship between stress and endocrine function is particularly critical to long-lived species such as sturgeons, whose economic viability relies heavily on proper endocrine function for the production of caviar. in this study, we examined the induced stress response, defined by plasma cortisol and glucose concentrations, and its relationship to plasma 17beta-estradiol, testos ...200717622217
distribution of galanin-like immunoreactivity in the brain of the siberian sturgeon (acipenser baeri).galanin is a 29-amino acid peptide widely distributed in the central nervous system of vertebrates. the organization of galaninergic systems is well known in teleosts, the most advanced actinopterygians, but no data are available on primitive bony fish. to extend the evolutionary analysis of galaninergic systems we studied the distribution of galanin-like immunoreactive (gal-ir) cells and fibers in the sturgeon brain, since chondrosteans are among the most primitive extant actinopterygians. doub ...200515861461
the application of image cytometry to viability assessment in dual fluorescence-stained fish spermatozoa.the viability of spermatozoa has been assessed using sybr 14 staining for dna of living cells and propidium iodide staining for dna of degenerate cells. this dual staining was performed on four fish species (siberian sturgeon, acipenser baerii; common carp, cyprinus carpio; tench, tinca tinca and wels, silurus glanis) and the proportions of live and dead spermatozoa were assessed by epifluorescence microscopy and image cytometry. ten phase contrast and epifluorescent images were recorded per sam ...200415566965
binding affinities of hepatic nuclear estrogen receptors for phytoestrogens in rainbow trout (oncorhynchus mykiss) and siberian sturgeon (acipenser baeri).phytoestrogens are dietary estrogenic contaminants capable of inducing vitellogenin synthesis in rainbow trout and siberian sturgeon. a competitive-binding assay on their hepatic estrogen receptors (er) was performed to determine the relative affinity of phytoestrogens compared to estradiol (e(2)). phytoestrogen concentrations used were 1000 times higher than for e(2), except for genistein and formononetin. for each compound, the competition with 50%-bound labelled e(2) (dc(50)) was considered i ...200212441116
glochidium metamorphosis in the endangered freshwater mussel margaritifera auricularia (spengler, 1793): a histological and scanning electron microscopy study.the metamorphosis of the glochidium of the critically endangered margaritifera auricularia in the gills of a host is studied here for the first time. siberian sturgeon, acipenser baeri, were infected with glochidia and regularly inspected using scanning and optical microscopy. the mature glochidia immediately attach to the epithelium of the sturgeon gill filaments, piercing the secondary lamellae and the connective tissues, blood cells, and vessels within the lamellae. once the epithelium is pie ...200212386896
[the influence of modulators on the membrane digestion process in siberian sturgeon acipenser baeri]. 200612070922
effects of dietary phytoestrogens in vivo and in vitro in rainbow trout and siberian sturgeon: interests and limits of the in vitro studies of interspecies differences.a study of the effects of dietary genistein on trout and sturgeon in vivo showed that sturgeon was sensitive to 20 ppm of genistein, whereas trout was not. to analyze the origin of this interspecies difference in sensitivity, a cell culture technique was developed with hepatocytes from sturgeon and compared to results obtained with hepatocytes from trout in the same system. the hepatocyte culture proved to be useful as bioassay for estrogenicity. vitellogenin (vtg), assayed by a specific enzyme- ...200211944965
acute exposure of siberian sturgeon (acipenser baeri, brandt) yearlings to nitrite: median-lethal concentration (lc(50)) determination, haematological changes and nitrite accumulation in selected tissues.exposure of siberian sturgeon (acipenser baeri) yearlings (172.0+/-18 g; mean+/-s.d.) to several no(2)(-)-n concentrations (0, 25, 130, 180 and 275 mg/l) was studied for 72 h in static tests. at 72 h, the median-lethal concentration of no(2)(-)-n was 130 mg/l in water with high chloride content (130.5 mg/l). nitrite exposure produced high levels of methaemoglobin (methb) but did not seem to cause mortality, as surviving fish showed higher levels (82.7+/-5.6%) than torpid specimens (60.8+/-4.5%). ...200211932005
[comparison of the effects of gonadotropic preparations of the carp and stellate sturgeon pituitaries on in vivo and in vitro oocyte maturation in the siberian sturgeon acipenser baeri brandt].injections of 2.5 mg/kg of stellate sturgeon (acipenser stellatus pall.) pituitary extract and 5 mg/kg of carp (cyprinus carpio l.) pituitary extract in siberian sturgeon (acipenser baeri brandt) females did not reveal significant differences in the effects of these preparations. there were no differences in the percentage of females that responded by ovulation, duration of the period from injection to ovulation, rate of ovulation, or quality of mature eggs as estimated by the percentage of fert ...200611605413
immunohistochemical distribution of cytochrome p4501a in larvae and fingerlings of the siberian sturgeon, acipenser baeri.in this paper we investigate by means of immunohistochemistry, the tissue distribution of constitutive cytochrome p4501a (cypia), from hatching until 30 days posthatching in developing siberian sturgeon, acipenser baeri. for this purpose, a polyclonal (bn-1) antiserum developed against a conservative sequence of piscine cyp1a and a monoclonal (c10-7) antiserum directed against cod cyp1a were used on paraffin-embedded samples. from hatching onwards, distinct cyp1a immunoreactivity was distinctly ...200111432638
distribution of choline acetyltransferase (chat) immunoreactivity in the central nervous system of a chondrostean, the siberian sturgeon (acipenser baeri).all studies to date of cholinergic systems of bony fishes have been done in teleosts. to gain further insight into the evolution of the cholinergic systems of bony fishes, we have studied the brain of a chondrostean fish, the siberian sturgeon (acipenser baeri, brandt), by using an antibody against choline acetyltransferase (chat). this study showed the presence of chat-immunoreactive (chat-ir) neurons in the preoptic region (parvocellular and magnocellular preoptic nuclei and suprachiasmatic nu ...200011027402
molecular genetic analysis among subspecies of two eurasian sturgeon species, acipenser baerii and a. stellatus.two species, the siberian sturgeon, acipenser baerii, and stellate sturgeon, a. stellatus, were studied using mitochondrial dna (mtdna) (d-loop, cytochrome b (cyt-b) and nd5/6 genes) sequencing to determine whether traditionally defined subspecies correspond to taxonomic entities and conservation management units. initially, several mtdna regions for each taxon (a. baerii: 737 bp d-loop, 750 bp nd5, 200 bp nd6, and 790 bp cyt-b; a. stellatus: 737 bp d-loop and 600 bp nd5) were examined. the d-lo ...199910703555
occurrence and in vitro biosynthesis of 11-ketotestosterone in siberian sturgeon, acipenser baeri brandt maturing females.high levels of 11-ketotestosterone (11kt) were found (49 to 160 ng ml(-1)) in plasma of siberian sturgeon females during the end of their reproductive cycle. these levels were measured either by specific radioimmunoassay, or both by specific radioimmunoassay and by uv absorption after hplc (isocratic conditions, 33% methanol, 26% acetonitrile, 41% water). in order to find the origin of 11kt synthesis, ovaries were incubated (30 min and 2h at 20°c) with tritiated 17-hydroxyprogesterone (17ohp) or ...199524197499
estrogenic effect of dietary soya bean meal on vitellogenesis in cultured siberian sturgeon acipenser baeri.the unusual presence of vitellogenin in the plasma of male and nonvitellogenic female siberian sturgeon has been demonstrated previously (pelissero and le menn, 1988; pelissero et al., 1989a) and was attributed to dietary effects. the present study examines estrogenic effects of dietary soya bean meal and of commercial trout diet on vitellogenesis in sturgeon. the 4-month study compared three diets, one commercial (t) and two experimental synthetic diets, one containing casein alone (sc for synt ...19911936926
first detection of pike fry-like rhabdovirus in barbel and spring viraemia of carp virus in sturgeon and pike in aquaculture in the czech republic.rapid antigen detection enzyme-linked immunosorbent assay (elisa) testing of cell cultures with organ homogenate from fish, collected from farms with a predominance of common carp or in natural aquaculture in the czech republic between 1995 and 2008, identified piscine vesiculovirus in 27 of 178 samples. using reverse transcription semi-nested pcr, targeting a 550 nucleotide region of the glycoprotein (g) gene, piscine vesiculovirus was confirmed in 23 of the 27 organ samples diagnosed by elisa ...201121848117
the effect of infestation with diplostomum sp. on growth rate of siberian sturgeon fry acipenser baeri brandt.the objective of the experiment was to compare the growth rate in two groups of siberian sturgeon fry, one of which was infested with metacercariae of diplostomum, the infestation degree was from 1 to 160/fish. the fish fed on natural as well as artificial food; the latter was given at the rate sufficient to cover 50% of the fish requirements. the average initial body weight in the control group was 16.8 +/- 1.9g, and in the group with diplostomum - 28.4 +/- 3.6 g. during the period of favorable ...200116888964
[occurrence of metacerkariae diplostomum sp. in the siberian turgeon fry (acipenser baeri brandt)--preliminary study].siberian sturgeon fry coming from three different ponds has been examined. 1. "dgał" - fish culture conerete-terrestrial pond beloning to d.o.z. dgał inland fisheries institute in olsztyn supplied with fresh water from great dgał lake; 2. "grzmieca" - fish culture pond terrestrial, natural belonging to fishing farm grzmieca near brodnica; 3. "konin" - fish culture pond near konin supplied with water utilized by near heat and power generating plant. total of 80 sturgeons fry with parameters: leng ...199916886375
topography and connections of the telencephalon in a chondrostean, acipenser baeri: an experimental study.sturgeons belong to an ancient group of the extant actinopterygian fishes. accordingly, the study of their brain connections is important to understand brain evolution in the line leading to teleosts. we examined the topography and connections of the various telencephalic regions of the siberian sturgeon (acipenser baeri). the telencephalic regions were characterized on the basis of acetylcholinesterase histochemistry and calbindin-d28k and calretinin immunohistochemistry. the telencephalic conn ...200616739163
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