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estrogenic effect of dietary soya bean meal on vitellogenesis in cultured siberian sturgeon acipenser baeri.the unusual presence of vitellogenin in the plasma of male and nonvitellogenic female siberian sturgeon has been demonstrated previously (pelissero and le menn, 1988; pelissero et al., 1989a) and was attributed to dietary effects. the present study examines estrogenic effects of dietary soya bean meal and of commercial trout diet on vitellogenesis in sturgeon. the 4-month study compared three diets, one commercial (t) and two experimental synthetic diets, one containing casein alone (sc for synt ...19911936926
the estrogenic activity of certain phytoestrogens in the siberian sturgeon acipenser baeri.various phytoestrogens such as formononetin, daidzein, genistein and equol were synthesized. their purity was assessed by various analytical techniques including melting point determination, thin-layer chromatography (tlc), infra-red spectra (i.r. spectra), nuclear magnetic resonance (1h- and 13c-nmr) and gas chromatography coupled with mass spectrometry (gc-ms). the estrogenic activity of these compounds, as well as biochanin a and coumestrol, was biologically tested by the induction of vitello ...19912009221
spectral characteristics of photoreceptors and horizontal cells in the retina of the siberian sturgeon acipenser baeri brandt.in the retina of siberian sturgeon, three spectral classes of photoreceptors were identified by microspectrophotometry. these were rods, oil drop-containing and oil drop-free cones possessing p549, and oil drop-containing cones possessing p613 and p465. with intracellular recordings, rod-driven, cone-driven, and mixed horizontal cells of luminosity type were found, as well as color-opponent horizontal cells of, at least, 6 kinds. biphasic r/g cells received hyperpolarizing input either from rods ...19911771790
immunocytochemical reactivity of rod and cone visual pigments in the sturgeon retina.microspectrophotometry and immunocytochemistry with several antivisual pigment antibodies were used to study visual cells of the siberian sturgeon, acipenser baeri brandt. the retina contained rods and three morphological types of cones: large cones with oil drops, small cones with oil drops, and cone-like cells without oil drops. rods and cone-like drop-free cells were found to possess porphyropsin-549, while the large oil drop-bearing cones contained red-sensitive (p613), green-sensitive (p542 ...19921534023
comparative distribution of mammalian gnrh (gonadotrophin-releasing hormone) and chicken gnrh-ii in the brain of the immature siberian sturgeon (acipenser baeri).the brain of the sturgeon has recently been shown to contain at least two forms of gnrh (gonadotropin-releasing hormone), mammalian gnrh (mgnrh) and chicken gnrh-ii (cgnrh-ii). in this study, we compared the distribution of immunoreactive (ir) mgnrh and cgnrh-ii in the brain of immature siberian sturgeons (acipenser baeri). the overall distribution of mgnrh was very similar to the distribution of sgnrh in teleosts such as salmonids or cyprinids. mgnrh-ir perikarya were observed in the olfactory ...19938288771
respiratory responses to progressive ambient hypoxia in the sturgeon, acipenser baeri.changes in respiratory and acid-base variables were studied in siberian sturgeon, acipenser baeri, during progressive deep hypoxia followed by recovery under normoxic conditions. during hypoxia, both ventilatory frequency and amplitude increased and this sturgeon was able to maintain standard oxygen consumption down to a low critical level of ambient po2 (pwo2 < 40 mmhg). during the posthypoxic period, an o2 debt was repaid by an elevated oxygen consumption (nearly double control value at 1 h), ...19938441872
occurrence and in vitro biosynthesis of 11-ketotestosterone in siberian sturgeon, acipenser baeri brandt maturing females.high levels of 11-ketotestosterone (11kt) were found (49 to 160 ng ml(-1)) in plasma of siberian sturgeon females during the end of their reproductive cycle. these levels were measured either by specific radioimmunoassay, or both by specific radioimmunoassay and by uv absorption after hplc (isocratic conditions, 33% methanol, 26% acetonitrile, 41% water). in order to find the origin of 11kt synthesis, ovaries were incubated (30 min and 2h at 20°c) with tritiated 17-hydroxyprogesterone (17ohp) or ...199524197499
circulatory and respiratory effects of an hypoxic stress in the siberian sturgeon.changes in circulatory, ventilatory and acid-base variables were studied in siberian sturgeon (acipenser baeri) exposed to acute and severe hypoxia (pwo2 = 10 torr), followed by a rapid return to normoxia. this treatment caused a significant stress, revealed by the high levels of plasma catecholamines and cortisol. the moderate circulatory changes firstly observed would represent the effects of increased plasma catecholamine levels together with an increased adrenergic nervous tone on the cardio ...19957481109
ascorbic acid status as affected by dietary treatment in the siberian sturgeon (acipenser baeri brandt): tissue concentration, mobilisation and l-gulonolactone oxidase activity.a study was conducted to evaluate tissue storage and mobilisation of l-ascorbic acid (aa) in the siberian sturgeon (acipenser baeri) fed three different experimental diets. the three treatments consisted of a diet devoid of vitamin c (diet a0) and two diets supplemented with equivalent of 300 mg aa kg(-1) in the form of either silicone-coated ascorbic acid (diet sc) or of ascorbyl-2-polyphosphate (diet ap). during the first phase (4 months) of the trial, six batches of 130 siberian sturgeon (ini ...199624194303
ig light chain gene in the siberian sturgeon (acipenser baeri).elasmobranch and teleost fish have their ig light (l) chain loci organized in multiple clusters (vl-jl-cl). the vl segments of teleosts are in opposite transcriptional orientation to the cl genes, suggesting that in teleosts and elasmobranchs there may have been separate evolutionary events leading to this organization. to address this problem, the igl locus from the siberian sturgeon (acipenser baeri) (representative of a branch between elasmobranchs and teleosts) was investigated. sequence ana ...19968757324
molecular genetic analysis among subspecies of two eurasian sturgeon species, acipenser baerii and a. stellatus.two species, the siberian sturgeon, acipenser baerii, and stellate sturgeon, a. stellatus, were studied using mitochondrial dna (mtdna) (d-loop, cytochrome b (cyt-b) and nd5/6 genes) sequencing to determine whether traditionally defined subspecies correspond to taxonomic entities and conservation management units. initially, several mtdna regions for each taxon (a. baerii: 737 bp d-loop, 750 bp nd5, 200 bp nd6, and 790 bp cyt-b; a. stellatus: 737 bp d-loop and 600 bp nd5) were examined. the d-lo ...199910703555
tgf-beta3 exists in bony fish.a partial nucleotide sequence of transforming growth factor-beta3 (tgf-beta3) has been isolated from the siberian sturgeon (acipenser baeri), rainbow trout (oncorhynchus mykiss) and european eel (anguilla anguilla), confirming a ubiquitous presence in the ray-finned (actinopterygian) bony fish. the bony fish tgf-beta3 is highly conserved, with some 83-84% nucleotide identity (coding region) and 90-95% predicted amino acid identity to known homeotherm tgf-beta3's. far lower homologies are apparen ...199910614492
characterization of beta2-microglobulin in a primitive fish, the siberian sturgeon (acipenser baeri). 199910541811
[occurrence of metacerkariae diplostomum sp. in the siberian turgeon fry (acipenser baeri brandt)--preliminary study].siberian sturgeon fry coming from three different ponds has been examined. 1. "dgał" - fish culture conerete-terrestrial pond beloning to d.o.z. dgał inland fisheries institute in olsztyn supplied with fresh water from great dgał lake; 2. "grzmieca" - fish culture pond terrestrial, natural belonging to fishing farm grzmieca near brodnica; 3. "konin" - fish culture pond near konin supplied with water utilized by near heat and power generating plant. total of 80 sturgeons fry with parameters: leng ...199916886375
variability of the immunoglobulin light chain in the siberian sturgeon, acipenser baeri.all sturgeon vl segments isolated in this study belong to a single family, vli, which can be divided into two subfamilies. of the 79 cdna clones isolated, 76 belong to the larger subfamily, vlia, and only 3 clones constitute the smaller subfamily, vlib. to evaluate variability, the shannon entropy was estimated for each individual amino acid position, and to facilitate comparisons of variability between species the mean entropy of the cdr regions was calculated. in such a comparison, the sturgeo ...199910579389
distribution of choline acetyltransferase (chat) immunoreactivity in the central nervous system of a chondrostean, the siberian sturgeon (acipenser baeri).all studies to date of cholinergic systems of bony fishes have been done in teleosts. to gain further insight into the evolution of the cholinergic systems of bony fishes, we have studied the brain of a chondrostean fish, the siberian sturgeon (acipenser baeri, brandt), by using an antibody against choline acetyltransferase (chat). this study showed the presence of chat-immunoreactive (chat-ir) neurons in the preoptic region (parvocellular and magnocellular preoptic nuclei and suprachiasmatic nu ...200011027402
immunohistochemical distribution of cytochrome p4501a in larvae and fingerlings of the siberian sturgeon, acipenser baeri.in this paper we investigate by means of immunohistochemistry, the tissue distribution of constitutive cytochrome p4501a (cypia), from hatching until 30 days posthatching in developing siberian sturgeon, acipenser baeri. for this purpose, a polyclonal (bn-1) antiserum developed against a conservative sequence of piscine cyp1a and a monoclonal (c10-7) antiserum directed against cod cyp1a were used on paraffin-embedded samples. from hatching onwards, distinct cyp1a immunoreactivity was distinctly ...200111432638
the effect of infestation with diplostomum sp. on growth rate of siberian sturgeon fry acipenser baeri brandt.the objective of the experiment was to compare the growth rate in two groups of siberian sturgeon fry, one of which was infested with metacercariae of diplostomum, the infestation degree was from 1 to 160/fish. the fish fed on natural as well as artificial food; the latter was given at the rate sufficient to cover 50% of the fish requirements. the average initial body weight in the control group was 16.8 +/- 1.9g, and in the group with diplostomum - 28.4 +/- 3.6 g. during the period of favorable ...200116888964
glyconjugates in epidermal, branchial and digestive mucous cells and gastric glands of gilthead sea bream, sparus aurata, senegal sole, solea senegalensis and siberian sturgeon, acipenser baeri development.epidermal, branchial and digestive mucous cells, and the gastric glands of larvae/postlarvae (from hatching until 45 days posthatching) of three fish species (two teleostean and a chondrostean) were investigated using conventional histochemical methods (periodic acid schiff -pas-, diastase-pas; alcian blue ph 0.5, 1 and 2.5) in order to distinguish neutral and acidic (carboxylated and sulphated) glycoconjugates, as well as bromophenol blue reaction for identification of proteins. additionally, t ...200111759813
binding affinities of hepatic nuclear estrogen receptors for phytoestrogens in rainbow trout (oncorhynchus mykiss) and siberian sturgeon (acipenser baeri).phytoestrogens are dietary estrogenic contaminants capable of inducing vitellogenin synthesis in rainbow trout and siberian sturgeon. a competitive-binding assay on their hepatic estrogen receptors (er) was performed to determine the relative affinity of phytoestrogens compared to estradiol (e(2)). phytoestrogen concentrations used were 1000 times higher than for e(2), except for genistein and formononetin. for each compound, the competition with 50%-bound labelled e(2) (dc(50)) was considered i ...200212441116
glochidium metamorphosis in the endangered freshwater mussel margaritifera auricularia (spengler, 1793): a histological and scanning electron microscopy study.the metamorphosis of the glochidium of the critically endangered margaritifera auricularia in the gills of a host is studied here for the first time. siberian sturgeon, acipenser baeri, were infected with glochidia and regularly inspected using scanning and optical microscopy. the mature glochidia immediately attach to the epithelium of the sturgeon gill filaments, piercing the secondary lamellae and the connective tissues, blood cells, and vessels within the lamellae. once the epithelium is pie ...200212386896
effects of dietary phytoestrogens in vivo and in vitro in rainbow trout and siberian sturgeon: interests and limits of the in vitro studies of interspecies differences.a study of the effects of dietary genistein on trout and sturgeon in vivo showed that sturgeon was sensitive to 20 ppm of genistein, whereas trout was not. to analyze the origin of this interspecies difference in sensitivity, a cell culture technique was developed with hepatocytes from sturgeon and compared to results obtained with hepatocytes from trout in the same system. the hepatocyte culture proved to be useful as bioassay for estrogenicity. vitellogenin (vtg), assayed by a specific enzyme- ...200211944965
acute exposure of siberian sturgeon (acipenser baeri, brandt) yearlings to nitrite: median-lethal concentration (lc(50)) determination, haematological changes and nitrite accumulation in selected tissues.exposure of siberian sturgeon (acipenser baeri) yearlings (172.0+/-18 g; mean+/-s.d.) to several no(2)(-)-n concentrations (0, 25, 130, 180 and 275 mg/l) was studied for 72 h in static tests. at 72 h, the median-lethal concentration of no(2)(-)-n was 130 mg/l in water with high chloride content (130.5 mg/l). nitrite exposure produced high levels of methaemoglobin (methb) but did not seem to cause mortality, as surviving fish showed higher levels (82.7+/-5.6%) than torpid specimens (60.8+/-4.5%). ...200211932005
early development of the olfactory organ in sturgeons of the genus acipenser: a comparative and electron microscopic study.formation and morphology of the olfactory organ of vertebrates has been intensely studied in some taxa for more than a century. as a functionally important and complex sensory organ, its ontogenetic development has often been a matter of debate on higher-level craniate evolution. however, sufficient knowledge of structure and development of the olfactory organ in the crucial taxa needed for a serious phylogenetic reasoning is generally not available. this study aims at this essential primary dat ...200312684762
[protein composition of the blood serum of 2 allopatric populations of the siberian sturgeon acipenser baeri brandt]. 20035397549
chromosomal mapping of 18s-28s and 5s rrna genes by two-colour fluorescent in situ hybridization in six sturgeon species.the number and distribution of the 18s-28s and 5s rrna (rdna) gene sequences were examined on mitotic chromosomes of six sturgeon species by two-colour in situ hybridization. four of the six species, huso huso, acipenser stellatus, acipenser sturio, and acipenser ruthenus, with about 120 chromosomes, showed from six to eight 18s-28s rdna signals, while 5s rdna signals were on only one chromosome pair. the two species with 250-270 chromosomes, acipenser baerii and acipenser transmontanus, showed ...200312834065
the application of image cytometry to viability assessment in dual fluorescence-stained fish spermatozoa.the viability of spermatozoa has been assessed using sybr 14 staining for dna of living cells and propidium iodide staining for dna of degenerate cells. this dual staining was performed on four fish species (siberian sturgeon, acipenser baerii; common carp, cyprinus carpio; tench, tinca tinca and wels, silurus glanis) and the proportions of live and dead spermatozoa were assessed by epifluorescence microscopy and image cytometry. ten phase contrast and epifluorescent images were recorded per sam ...200415566965
distribution of galanin-like immunoreactivity in the brain of the siberian sturgeon (acipenser baeri).galanin is a 29-amino acid peptide widely distributed in the central nervous system of vertebrates. the organization of galaninergic systems is well known in teleosts, the most advanced actinopterygians, but no data are available on primitive bony fish. to extend the evolutionary analysis of galaninergic systems we studied the distribution of galanin-like immunoreactive (gal-ir) cells and fibers in the sturgeon brain, since chondrosteans are among the most primitive extant actinopterygians. doub ...200515861461
topography and connections of the telencephalon in a chondrostean, acipenser baeri: an experimental study.sturgeons belong to an ancient group of the extant actinopterygian fishes. accordingly, the study of their brain connections is important to understand brain evolution in the line leading to teleosts. we examined the topography and connections of the various telencephalic regions of the siberian sturgeon (acipenser baeri). the telencephalic regions were characterized on the basis of acetylcholinesterase histochemistry and calbindin-d28k and calretinin immunohistochemistry. the telencephalic conn ...200616739163
[a research on ig heavy chain constant region of five acipenseridae].analyzed the immunoglobulin (ig) heavy chain constant regions of russian sturgeon (a. gueldenstaedtii); sterlte sturgeon (a. ruthenus); amur sturgeon (a. schrenckii); chinese sturgeon (a. sinensis) and great sturgeon (huso huso) with molecular biology and bioinformatics methods. we cloned igh nucleic acid sequences by rt-pcr using the specific primer, then determined the characteristics and functions of the amino acid sequences and plotted out the sequence into four sub-regions (ch1-ch4), of whi ...200617035183
[comparison of the effects of gonadotropic preparations of the carp and stellate sturgeon pituitaries on in vivo and in vitro oocyte maturation in the siberian sturgeon acipenser baeri brandt].injections of 2.5 mg/kg of stellate sturgeon (acipenser stellatus pall.) pituitary extract and 5 mg/kg of carp (cyprinus carpio l.) pituitary extract in siberian sturgeon (acipenser baeri brandt) females did not reveal significant differences in the effects of these preparations. there were no differences in the percentage of females that responded by ovulation, duration of the period from injection to ovulation, rate of ovulation, or quality of mature eggs as estimated by the percentage of fert ...200611605413
[the influence of modulators on the membrane digestion process in siberian sturgeon acipenser baeri]. 200612070922
ig heavy chain of the sturgeon acipenser baeri: cdna sequence and diversity.to investigate the gene organization of the igh locus, and the vh diversity of the siberian sturgeon, a cdna library was constructed and screened with vh-specific probes from two holostean fish. isolated clones were analyzed and domain-specific probes used in rescreening of the library, southern blot analysis, and northern blots. it was concluded that the siberian sturgeon has one igh locus with a translocon type of organization. two allelic variants of the mu gene were found, with identities ra ...20069799333
evolutionary aspects of intestinal bicarbonate secretion in fish.experiments compared intestinal hco3- secretion in the intestine of marine teleost gulf toadfish, opsanus beta, to representatives of early chondrostean and chondrichthyan fishes, the siberian sturgeon, acipenser baerii, and white-spotted bamboo shark, chiloscyllium plagiosum, respectively. as seen in marine teleosts, luminal hco3- concentrations were 10-fold plasma levels in all species when exposed to hyperosmotic conditions. while intestinal water absorption left mg2+ and so4(2-) concentrated ...200616503178
availability of in vitro vitellogenin assay for screening of estrogenic and anti-estrogenic activities of environmental chemicals.vitellogenin (vtg) protein, vtg mrna, other egg yolk proteins, vitelline envelope proteins and their mrnas are produced in the liver of oviparous species by stimulation of endogenous estrogen and exogenous estrogenic chemicals. the vtg assay based on enzyme-linked immunosorbent assay (elisa) has been widely used for many fish species to screen estrogenic and anti-estrogenic activities of chemicals and sewage effluents using immature fish and/or male fish. in order to reduce the number of fish fo ...200616883298
dietary ascorbic acid may be necessary for enhancing the immune response in siberian sturgeon (acipenser baerii), a species capable of ascorbic acid biosynthesis.siberian sturgeon (acipenser baerii brandt) is one of few fish species capable of synthesizing ascorbic acid (aa); however, it is unknown whether dietary aa is still necessary for this species to generate an optimal non-specific immune response, as is found in most teleostean species that do not synthesize aa. sturgeon fed either 0 or 500 mg aa (aa polyphosphate) equivalent kg(-1) diets for 2 weeks were then either sham or experimentally injected with aeromonas hydrophila lipopolysaccharide (lps ...200616901741
disposition of genistein in rainbow trout (oncorhynchus mykiss) and siberian sturgeon (acipenser baeri).genistein (g) is a xenoestrogen from soy present in fish diet. in vivo, a 50-fold difference in sensitivity to genistein on vitellogenin (vtg) synthesis was found when comparing trout and sturgeon. this difference was not linked to the estrogen receptor affinity nor to the sensitivity of induction of the vtg pathway. the study was performed to check if differences in the g disposition in the two species could explain their difference of sensitivity to g. a pharmacokinetic analysis of radiolabele ...200717097654
morphology and ultrastructure of siberian sturgeon (acipenser baerii) spermatozoa using scanning and transmission electron microscopy.available data concerning the sperm morphology of teleost fishes demonstrate wide variation. in the present study, the spermatozoa of siberian sturgeon (acipenser baerii brandt, 1869), a chondrostean fish, was investigated. in contrast with teleost fish, chondrostean spermatozoa have a head with a distinct acrosome, whereas other structures, such as a midpiece and a single flagellum, are present in spermatozoa of most species.200717014420
stress and its relation to endocrine function in captive female siberian sturgeon (acipenser baeri).stress responses to numerous environmental conditions have been studied in a wide range of fish species. defining the relationship between stress and endocrine function is particularly critical to long-lived species such as sturgeons, whose economic viability relies heavily on proper endocrine function for the production of caviar. in this study, we examined the induced stress response, defined by plasma cortisol and glucose concentrations, and its relationship to plasma 17beta-estradiol, testos ...200717622217
immunoglobulin heavy chain constant region of five acipenseridae: cdna sequence and evolutionary relationship.the immunoglobulin m (igm) heavy chain constant region genes of russian sturgeon (acipenser gueldenstaedtii), sterlte sturgeon (acipenser ruthenus), amur sturgeon (acipenser schrenckii), chinese sturgeon (acipenser sinensis) and great sturgeon (huso huso) were cloned and analyzed with molecular biology and bioinformatics methods. we cloned igh nucleic acid sequences by rt-pcr using the specific primer, then determined the characteristics and functions of the amino acid sequences. the gene contai ...200717234432
[molecular identification and the features of genetic diversity in interspecific hybrids of amur sturgeon (acipenser schrenckii x a. baerii, a. baerii x a. schrenckii, a. schrenckii x a. ruthenus, and a. ruthenus x a. schrenckii) based on variability of multilocus rapd markers].the method of polymerase chain reaction with random primers (rapd pcr) was used to identify the progeny of the crosses between three sturgeon species, amur sturgeon (acipenser schrenckii brandt, 1869), siberian sturgeon (a. baerii brandt, 1869), and sterlet (a. ruthenus linnaeus, 1758). using ten primers, genetic variation in 70 yearlings, produced in seven individual crosses: acipenser schrenckii x a. schrenckii, a. baerii x a. baerii, a. ruthenus x a. ruthenus, a. schrenckii x a. baerii, a. ba ...200819137727
long-term culture of a cell population from siberian sturgeon (acipenser baerii) head kidney.in vitro cultures of native fish cell lines are of great importance, both for basic research and applied science. in particular, there is strong demand for long-term growable cell lines from breeding fish, like sturgeon. here, we describe the culture of cells from siberian sturgeon (acipenser baerii) head kidney. the cells have so far been cultured over a period of 12 months (24 passages). cytochemical and immunocytochemical examination suggests that, in vitro, the cells exhibit markers that are ...200818958594
[polymorphism of the mitochondrial dna control region in eight sturgeon species and development of a system for dna-based species identification].intraspecific and interspecific nucleotide sequence variations of the mtdna control region (d-loop) were studied with mtdnas isolated from tissue specimens of more than 1400 sturgeons of nine species: russian sturgeon acipenser gueldenstaedtii, persian sturgeon a. persicus, siberian sturgeon a. baerii, amur sturgeon a. schrenkii, fringebarbel sturgeon a. nudiventris, sterlet a. ruthenus, stellate sturgeon a. stellatus, beluga huso huso, and kaluga h. dauricus. the results were used to analyze th ...200818767539
the optimization of the protocol for immunofluorescence on fish spermatozoa.in comparison with mammals, the fertilization of fish occurs predominantly outside the organism in a water environment, where fish spermatozoa require specific conditions to interact with oocytes. it is evident that optimal conditions for fish and mammalian spermatozoa are quite different. this paper describes a special approach to handling fish (common carp and siberian sturgeon) spermatozoa in comparison with the samples originating from mammals (boar). this approach concerns not only the diff ...200818584860
the australian lungfish (neoceratodus forsteri) - fish or amphibian pattern of muscle development?the australian lungfish neoceratodus forsteri (dipnoi-sarcoterygians) is a likely candidate for the extant sister group of tetrapoda. transmission electron and light microscopy analysis revealed that the arrangement of somite cells of the lungfish resembles the structure of the urodelan somite. on the other hand, the pattern of early muscle formation in n. forsteri is similar to that found in the siberian sturgeon (acipenser baeri). during the early stages of myogenesis of n. forsteri, somite-de ...200818311718
the 'storm of the century' (december 1999) and the accidental escape of siberian sturgeons (acipenser baerii) into the gironde estuary (southwest france). an original approach for metal contamination.the gironde estuary is considered as a reference ecosystem with a large fish assemblage (75 species) and the presence of eleven diadromous species. however, geochemical studies and biomonitoring using oysters have shown high metal pollution levels. during the december 1999 hurricane, more than 5,000 young siberian sturgeons (acipenser baerii) accidentally escaped from a fish farm into the estuary, where this species had not been present until then.200818306893
coamplification of eukaryotic dna with 16s rrna gene-based pcr primers: possible consequences for population fingerprinting of complex microbial communities.the main aim of this study was to evaluate the specificity of three commonly used 16s rrna gene-based polymerase chain reaction (pcr) primer sets for bacterial community analysis of samples contaminated with eukaryotic dna. the specificity of primer sets targeting the v3, v3-v5, and v6-v8 hypervariable regions of the 16s rrna gene was investigated in silico and by community fingerprinting of human and fish intestinal samples. both in silico and pcr-based analysis revealed cross-reactivity of the ...200818301945
distribution of somatostatin immunoreactive neurons and fibres in the central nervous system of a chondrostean, the siberian sturgeon (acipenser baeri).somatostatin (som) is a neuropeptide that is widely distributed in the central nervous system of vertebrates. two isoforms of somatostatin (ss1 and ss2) have been characterized in sturgeon and in situ hybridisation studies in the sturgeon brain have demonstrated that mrnas of the two somatostatin precursors (pss1 and pss2) are differentially expressed in neurons [trabucchi, m., tostivint, h., lihrmann, i., sollars, c., vallarino, m., dores, r.m., vaudry, h., 2002. polygenic expression of somatos ...200818400215
malformations of the endangered chinese sturgeon, acipenser sinensis, and its causal agent.the anadromous chinese sturgeon (acipenser sinensis) is endangered and listed among the first class of protected animals in china. the possible causes for the decline of this species are the effects of synthetic chemicals, and loss of critical habitat. chinese sturgeon in the yangtze river have accumulated triphenyltin (tpt) to 31-128 ng/g wet weigh (ww) in liver, which is greater than the concentrations of tributyltin (<1.0 ng/g ww). maternal transfer of tpt has resulted in concentrations of 25 ...200919470453
first detection of a viral agent causing disease in farmed sturgeon in russia.a virus was isolated from siberian sturgeon acipenser baeri and bester (beluga huso huso x sterlet a. ruthenus hybrid) fingerlings in sso-2, ssf-2 and wssk-1 cell lines during an acute epizootic on a large fish farm producing fertilised sturgeon eggs and fry. transmission electron microscopic examination of samples from both inoculated cell cultures and skin of affected fish revealed viral particles with a herpesvirus-like morphology. the etiological role of the siberian sturgeon herpesvirus (sb ...200920066954
[isolation and identification of pathogenic aeromonas veronii isolated from infected siberian sturgeon (acipenser baerii)].our study aimed at searching for the pathogenic factor causing siberian sturgeon (acipenser baerii) disease.200920069873
diverse splicing pathways of the membrane ighm pre-mrna in a chondrostean, the siberian sturgeon.teleosts and tetrapods have evolved different splice patterns to generate their membrane-bound igm. in the tetrapod lineage, the first transmembrane exon is spliced to an internal cryptic site located close to the end of the fourth constant exon. because teleosts lack this site they use the regular 3'-splice site of the ch3 exon instead. we characterized the mum splicing patterns in a chondrostean, the siberian sturgeon. we observed a surprising diversity of splice patterns, the tm1 exon being s ...200919027787
the bioaccumulation of fluoride ion (f(-)) in siberian sturgeon (acipenser baerii) under laboratory conditions.a 90-d growth trial was conducted to determine the accumulation of fluoride ions (f(-)) in juvenile siberian sturgeon (acipenser baerii). fish were exposed to nominal f(-) concentrations of 4, 10, 25, 62.5 mg l(-1) (added as naf), along with a control group. fish growth was determined and f(-) concentrations in bone, cartilage, skin, gill, muscle, liver, gut and pylorus were analyzed by fluoride specific electrode method. fish growth was inhibited for groups exposed to 10, 25, and 62.5 mg f(-) l ...200919150727
testing taxonomic boundaries and the limit of dna barcoding in the siberian sturgeon, acipenser baerii.dna barcoding efforts involving animals have focused on the mitochondrial cytochrome c oxidase subunit i (cox1) gene. some authors suggest that this marker might under-diagnose young species. herein, we examine cox1 and control region diversity in a sample of siberian sturgeon (acipenser baerii), a species with an extremely wide geographic distribution in the major rivers of siberia and in lake baikal. some authors currently recognize three subspecies within this species. these subspecies are re ...200919900060
[effects of initial feeding on the growth, survival, and body biochemical composition of siberian sturgeon (acipenser baerii) larvae].siberian sturgeon (acipenser baerii) larvae were raised with limnodrilus sp., artemia nauplii, moina sp., and artificial commercial diet for 30 days to investigate the effects of different initial feeding diets on the growth, survival, and body biochemical composition of the larvae. the results showed that a. nauplii was the best initial feeding diet, with the survival rate of a. baerii larvae being significant higher (96.67%) than the others. feeding with limnodrilus sp. gave the highest growth ...200919459376
ultrastructural study on the fertilisation process in sturgeon (acipenser), function of acrosome and prevention of polyspermy.sturgeon gametes differ from other fish in that their spermatozoa possess acrosome with finger-like posterolateral projections, which undergo exocytosis and filament formation, whereas eggs possess numerous micropyles. the fertilisation process in acipenser baerii was investigated by fluorescence and electron microscopy. a suitable activation solution containing 2.5 mm cacl(2), 15 mm tris, ph 10 was found for detailed description of acrosomal reaction. the acrosome reaction includes the formatio ...201019410390
comparative protein profiles: potential molecular markers from spermatozoa of acipenseriformes (chondrostei, pisces).sturgeon and paddlefish (acipenseriformes), the source of roe consumed as caviar, are a unique and commercially valuable group of ancient fishes. in this study, comparative proteomics was used to analyze protein profiles of spermatozoa from five sturgeon species and one paddlefish: siberian sturgeon (acipenser baerii), sterlet (a. ruthenus), russian sturgeon (a. gueldenstaedtii), starry sturgeon (a. stellatus), beluga (huso huso), and mississippi paddlefish (polyodon spathula). protein profiles ...201020869341
[discrete phenotypical variability of electrophoretic specters of one of myogen systems in the acipenseridae family].analysis of electrophoregrams of water-soluble proteins of white skeletal muscle of 10 fish species belonging to three genera of the acipenseridae family has allowed revealing an earlier not described system that can serve as a biochemical marker. this protein system represents a set of five fractions (a, b, c, d, e). a part of the studied species is monomorphous for one of these fractions (sterlet, starred sturgeon, great sturgeon, and big shovelnose had phenotype a, barbel sturgeon--phenotype ...201020799609
[effects of storage medium and temperature on short-term storage of acipenser baerii eggs].this paper studied the effects of different storage medium (coelomic fluid, hepes solution, and modified ringer's solution), temperature (4 degrees c and 16 degrees c), and duration (4 h, 8 h, 16 h, and 24 h) on the short-term storage of siberian sturgeon (acipenser baerii) eggs. the results showed that storage medium, temperature, and duration all had significant effects on the fertilization rate, hatching success, and malformation of newly hatched larvae (p < 0.05). with the increase of storag ...201020387448
partial genome analysis of siberian sturgeon alloherpesvirus suggests its close relation to acihv-2 - short communication.partial genome sequence of a herpes-like virus, isolated from siberian sturgeon (acipenser baeri), was determined and subjected to phylogenetic analysis. the virus (sbshv) has been shown to be the causative agent of an acute disease with high mortality in farmed juvenile sturgeons in russia. two fragments (of 7000 and 300 base pairs in length) encompassing 3 complete and 3 partial orfs were amplified by pcr. sturgeon herpesvirus strains, classified into species acipenserid herpesvirus 2 (acihv-2 ...201020460226
phb-degrading bacteria isolated from the gastrointestinal tract of aquatic animals as protective actors against luminescent vibriosis.the use of poly-β-hydroxybutyrate (phb) was shown to be successful in increasing the resistance of brine shrimp against pathogenic infections. in this study, we isolated for the first time phb-degrading bacteria from a gastrointestinal environment. pure strains of phb-degrading bacteria were isolated from siberian sturgeon, european sea bass and giant river prawn. the capability of selected isolates to degrade phb was confirmed in at least two of three setups: (1) growth in minimal medium contai ...201020597982
first detection of pike fry-like rhabdovirus in barbel and spring viraemia of carp virus in sturgeon and pike in aquaculture in the czech republic.rapid antigen detection enzyme-linked immunosorbent assay (elisa) testing of cell cultures with organ homogenate from fish, collected from farms with a predominance of common carp or in natural aquaculture in the czech republic between 1995 and 2008, identified piscine vesiculovirus in 27 of 178 samples. using reverse transcription semi-nested pcr, targeting a 550 nucleotide region of the glycoprotein (g) gene, piscine vesiculovirus was confirmed in 23 of the 27 organ samples diagnosed by elisa ...201121848117
Effects of poly-ß-hydroxybutyrate (PHB) on Siberian sturgeon (Acipenser baerii) fingerlings performance and its gastrointestinal tract microbial community.Poly-ß-hydroxybutyrate (PHB) is a natural polymer that can be depolymerized into water-soluble short-chain fatty acid monomers. These monomers can act as microbial control agents. In this study, the effects of partially replacing the diet of Siberian sturgeon fingerlings with 2% and 5% PHB were investigated. Replacing 2% of the diet with PHB improved weight gain, specific growth rate (SGR) and survival in the sturgeon fingerlings during the 10-week experimental period. Community-level physiologi ...201122066757
[impacts of ultraviolet irradiation on the sperm motility and longevity of acipenser baerii].this paper studied the impacts of different dose ultraviolet irradiation (254 nm, uvc) on the sperm motility and longevity of acipenser baerii. ultraviolet irradiation had significant impacts on the sperm motility, its fast motion time, and longevity. with the increasing dose of ultraviolet irradiation, the sperm motility decreased rapidly first, increased rapidly then, and decreased rapidly again. the sperm fast motion time had the similar variation trend as the sperm motility, but the sperm lo ...201122097385
anatomical relations between neuropeptide y, galanin, and gonadotropin-releasing hormone in the brain of chondrostean, the siberian sturgeon acipenser baeri.we investigated the immunohistochemical localization of immunoreactive (ir) cell bodies and fibers of neuropeptide y (npy) and galanin (gal), and the anatomical relations between these neurons in the brain of the siberian sturgeon acipenser baeri to clarify the interactions between these neuropeptides. furthermore, the anatomic relations between npy and gonadotropin-releasing hormone (gnrh) in the brain were also examined. npy-ir cell bodies were observed in the ventral part of the ventral telen ...201121871949
persistence of environmental dna in freshwater ecosystems.the precise knowledge of species distribution is a key step in conservation biology. however, species detection can be extremely difficult in many environments, specific life stages and in populations at very low density. the aim of this study was to improve the knowledge on dna persistence in water in order to confirm the presence of the focus species in freshwater ecosystems. aquatic vertebrates (fish: siberian sturgeon and amphibian: bullfrog tadpoles) were used as target species. in control ...201121858099
[dispermic androgenesis in sturgeons with the help of cryopreserved sperm: production of androgenetic hybrids between siberian and russian sturgeons].dispermic androgenesis was used to produce, for the first time, an androgenetic progeny of the siberian sturgeon (acipenser baerii) and the androgenetic nuclear cytoplasmic hybrids (siberian sturgeon, a. baerii x russian sturgeon, a. gueldenstaedtii) using cryopreserved sperm. microsatellite dna analysis confirmed exclusively paternal inheritance in the androgenetic progeny of siberian sturgeon. heterozygotes for certain microsatellite loci were detected among the androgenetic hybrids, thereby c ...201121542342
distribution of glycine immunoreactivity in the brain of the siberian sturgeon (acipenser baeri): comparison with γ-aminobutyric acid.glycine and γ-aminobutyric acid (gaba) are the main inhibitory neurotransmitters in the central nervous system (cns) of vertebrates. studies on the distribution of glycinergic neurons and fibers have been carried out mainly in rodents and lampreys. with the aim of discovering more about the early evolution of this system in vertebrates, we analyzed the distribution of glycine-immunoreactive (gly-ir) neurons and fibers in the cns of a basal ray-finned fish, the siberian sturgeon (chondrostei, aci ...201121344405
magnitude of the root effect in red blood cells and haemoglobin solutions of fishes: a tribute to august krogh.the ability of high carbon dioxide tensions or low ph to reduce blood oxygen binding even at high oxygen tensions, first observed by august krogh and isabella leitch in 1919 and now known as the root effect, was studied in red blood cells and haemoglobin solutions of several fish species.201121199396
non-contact doppler radar monitoring of cardiorespiratory motion for siberian sturgeon.this paper presents the first reported use of doppler radar to remotely sense heart and ventilation rates of fish. the radar reported 35 to 40 bpm heart rate and 115 to 145 bpm ventilation rates for siberian sturgeon, with agreement from a video reference. conventional fish vital signs measurements require invasive surgery and human handling--these are problematic for large scale monitoring, for measuring deep sea fish, and other situations which preclude human interaction with each individual s ...201223366402
effects of arabinoxylan-oligosaccharides (axos) on juvenile siberian sturgeon (acipenser baerii) performance, immune responses and gastrointestinal microbial community.arabinoxylan-oligosaccharides (axos) are a newly discovered class of candidate prebiotics that exert different properties depending on their structure. in this study the effects of two different structures of axos, namely axos-32-0.30 (average degree of polymerization: 32, average degree of substitution: 0.30) and axos-3-0.25, were investigated on growth performance, immune responses, gut microbial fermentation and gut bacterial composition of juvenile siberian sturgeon (acipenser baerii). after ...201222796425
[development of the lateral line system in juvenile siberian sturgeon (acipenser baerii)].the siberian sturgeon (acipenser baerii brandt), a chondrostean, occupies an important position in the evolution of the electroreceptor. in order to more fully understanding the evolution of these receptors, we examined the development of the lateral line system during early ontogeny of the siberian sturgeon by using light and scanning electron microscopes. we detected four major events in this process: the lateral line placodal development, the sensory ridge formation, the receptor formation an ...201222653853
expression and phylogeny of candidate genes for sex differentiation in a primitive fish species, the siberian sturgeon, acipenser baerii.the molecular mechanisms underlying testis differentiation in basal actinopterygian fish remains poorly understood. the sex differentiation period was investigated in the siberian sturgeon, acipenser baerii, by expression profiling of sertoli cell transcription factors (dmrt1, sox9) that control testis differentiation in vertebrates; leydig cell factors (cyp17a1, star) affecting androgen production; the androgen receptor (ar); a growth factor controlling testis development (igf1); and a gene cod ...201222618959
[cloning and expression of tbx3 gene in siberian sturgeon, acipenser baerii].tbx3, a member of the tbx2 subfamily of t-box gene family, encodes a transcription factor with a highly conserved dna-binding domain, which called t-domain. tbx3 is involved in morphogenesis and organogenesis in vertebrates, such as limb development, heart remodeling, and neural placode differentiation. in the present study, a full-length 2 908 bp tbx3 cdna from acipenser baerii (abtbx3) was obtained using rt-pcr and race technique, which includes a 2 166 bp complete open reading frame encoding ...201222467390
mitochondrial genome of the siberian sturgeon acipenser baerii.the complete mitochondrial genome of siberian sturgeon acipenser baerii had been determined. the total length of the mitogenome was 16,763 bp and had 13 protein-coding, 22 trna, and 2 rrna genes. except for the eight trna and nd6 genes, all other mitochondrial genes are encoded on the heavy strand. the overall base composition of the heavy strand is 30.3% a, 24.3% t, 29.1% c, 16.3% g, with an at content of 54.6%. the dna sequence of a. baerii shared 97.8, 97.6, 93.5, 91.6, 93.6, and 87.1% sequen ...201222409751
morphological structure and peripheral innervation of the lateral line system in the siberian sturgeon (acipenser baerii).light and scanning electron microscopy (sem) were used to study the epidermal lateral line system of the siberian sturgeon (acipenser baerii brandt, 1869). this system consists of mechanoreceptive neuromasts, ampullae and the electroreceptive organ. the neuromasts are located in 5 pairs of cephalic and 1 pair of trunk canals and superficially in the middle and posterior pit lines that lie dorsomedially along the top of the skull immediately adjacent to the otic ampullae field. both canal neuroma ...201222405451
use of electrosense in the feeding behavior of sturgeons.the electro-receptive lateral line system appeared early in the evolutionary history of fish. sturgeons, members of the primitive chondrostei group, are known for their electroreceptors (ampullae of lorenzini) on the head, which are thought to be sensitive to weak electric fields in aquatic environments and involved in feeding, mating and migration. here, we report the results of a set of behavioral and electrophysiological experiments designed to determine the function and characteristics of th ...201222405450
effects of dietary bovine lactoferrin on growth, physiological performance, iron metabolism and non-specific immune responses of siberian sturgeon acipenser baeri.the present experiment was carried out to investigate the effects of different levels of dietary lactoferrin (lf) on growth performance, physiological status, iron absorption and innate immune response of juvenile siberian sturgeon acipenser baeri. fish were fed with six different rations including 0, 100, 200, 400, 800 and 1600 mg lf kg(-1) diet for 8 weeks. at the end of the experiment, samples were collected for estimating the physiological and immunological parameters. dietary lf did not cha ...201222377421
immunohistochemical study of the distribution of calcium binding proteins in the brain of a chondrostean (acipenser baeri).chondrosteans represent an ancient lineage in ray-finned bony fishes and hence in jawed vertebrates. this immunohistochemical study in the brain of the siberian sturgeon reports the neuronal distribution of three cytosolic calcium-binding proteins: calbindin-d28k (cb), calretinin (cr), and parvalbumin (pv). cb and cr are widely expressed in different neuron subsets distributed throughout the sturgeon brain. studies using double immunofluorescence reveal a wide co-distribution of cb and cr in the ...201222173872
molecular cytogenetics in artificial hybrid and highly polyploid sturgeons: an evolutionary story narrated by repetitive sequences.we applied comparative genomic hybridization (cgh) and genomic in situ hybridization (gish) to examine genomes of artificially produced sturgeon hybrids between sterlet, acipenser ruthenus female (∼120 chromosomes) or russian sturgeon, a. gueldenstaedtii female (∼240 chromosomes) and a spontaneous triploid siberian sturgeon a. baerii male (∼360 chromosomes), respectively. the ploidy levels of progenies were analyzed by karyotyping and flow cytometry. we found that the species-specific regions we ...201324051427
antioxidant response versus selenium accumulation in the liver and kidney of the siberian sturgeon (acipenser baeri).the aim of this study was to examine the effects of selenium on concentrations of metabolites and enzyme activities acting as antioxidant markers in liver and kidney of siberian sturgeon acipenser baeri. sturgeons were fed selenium cysteine for 30 and 60 d at 1.25, 5, 20mg sekg(-1). selenium level in the control feed was 0.32 mg kg(-1). se concentration was measured in liver, kidney and muscle of every specimen. sturgeon accumulated se in tissues with a clear dose-response relationship and the h ...201324034889
[the use of microsatellite loci for identification of sturgeon species (acipenseridae) and hybrid forms].the genetic polymorphism often sturgeon species that inhabit the territory of the russian federation (russian sturgeon, siberian sturgeon, amur sturgeon, sakhalin sturgeon, persian sturgeon, ship sturgeon, starlet, stellate sturgeon, beluga, and kaluga) was examined at five microsatellite loci (afug41, afug51, an20, aoxd161, aoxd165) (in total, 3821 individuals). the examined loci were successfully amplified with the same primer set in all species tested and demonstrated a high level of variatio ...201325508908
[the use of microsatellite loci for identification of sturgeon species (acipenseridae) and hybrid forms].the genetic polymorphism often sturgeon species that inhabit the territory of the russian federation (russian sturgeon, siberian sturgeon, amur sturgeon, sakhalin sturgeon, persian sturgeon, ship sturgeon, starlet, stellate sturgeon, beluga, and kaluga) was examined at five microsatellite loci (afug41, afug51, an20, aoxd161, aoxd165) (in total, 3821 individuals). the examined loci were successfully amplified with the same primer set in all species tested and demonstrated a high level of variatio ...201325486777
glycine-immunoreactive neurons in the brain of a shark (scyliorhinus canicula l.).the glycinergic cell populations in the brain of the lesser spotted dogfish were studied by a glycine immunofluorescence method. numerous glycine-immunoreactive (gly-ir) neurons were observed in different brain nuclei. in the telencephalon, gly-ir cells were observed in the olfactory bulb, telencephalic hemispheres, and preoptic region. in the hypothalamus, cerebrospinal fluid-contacting gly-ir neurons were observed in the lateral and posterior recess nuclei. coronet cells of the saccus vasculos ...201323630026
effect of short-term storage on quality parameters, dna integrity, and oxidative stress in russian (acipenser gueldenstaedtii) and siberian (acipenser baerii) sturgeon sperm.the sperm of russian sturgeon (a. gueldenstaedtii) and siberian sturgeon (a. baerii) was used to evaluate the effects of short-term (liquid) storage on functional parameters (spermatozoa motility and velocity), dna integrity and oxidative stress indices. spermatozoa showed >50% motility during 6 days of storage with an average velocity of 133.12±15.4 to 87.9±11.23μm s(-1) in both species. no motile spermatozoa were recorded after nine days of storage. analysis of russian sturgeon sperm showed no ...201323602487
prebiotic effects of arabinoxylan oligosaccharides on juvenile siberian sturgeon (acipenser baerii) with emphasis on the modulation of the gut microbiota using 454 pyrosequencing.the potential of a novel class of prebiotics, arabinoxylan oligosaccharides (axos), was investigated on growth performance and gut microbiota of juvenile acipenser baerii. two independent feeding trials of 10 or 12 weeks were performed with basal diets supplemented with 2% or 4% axos-32-0.30 (trial 1) and 2% axos-32-0.30 or axos-3-0.25 (trial 2), respectively. growth performance was improved by feeding 2% axos-32-0.30 in both trials, although not significantly. microbial community profiles were ...201323786549
effects of dietary arabinoxylan-oligosaccharides (axos) and endogenous probiotics on the growth performance, non-specific immunity and gut microbiota of juvenile siberian sturgeon (acipenser baerii).we investigated the effects of administration of putative endogenous probiotics lactococcus lactis spp. lactis or bacillus circulans, alone and in combination with arabinoxylan-oligosaccharides (axos), a new class of candidate prebiotics, in juvenile siberian sturgeon (acipenser baerii). eight experimental diets were tested: basal diet (diet 1), basal diet supplemented with 2% axos (diet 2), or l. lactis st g81 (diet 3), l. lactis st g45 (diet 4), b. circulans st m53 (diet 5), l. lactis st g81 + ...201323811408
expression of dmrt1 and sox9 during gonadal development in the siberian sturgeon (acipenser baerii).the sex differentiation period of the siberian sturgeon was investigated through expression profiling of two testicular markers (dmrt1 and sox9). at the molecular level, a clear sexual dimorphism of dmrt1 and sox9 was observed in 3-year-old fish with immature gonads, in which males showed higher expression of these genes. among 16-month-old sturgeons cultured in uruguay, gonad morphology analyses showed one group of fish with undifferentiated gonads and a second group which had started their his ...201322688449
fins improve the swimming performance of fish sperm: a hydrodynamic analysis of the siberian sturgeon acipenser baerii.the flagella of sturgeon sperm have an ultrastructure comprising paddle-like fins extending along most of their length. these fins are seen in several other marine and freshwater fish. the sperm of these fish are fast swimmers and are relatively short lived: it is therefore tempting to think of these fins as having evolved for hydrodynamic advantage, but the actual advantage they impart, at such a small length scale and slow speed, is unclear. the phrase "the fins improve hydrodynamic efficiency ...201323233331
new disease records for hatchery-reared sturgeon. ii. phaeohyphomycosis due to veronaea botryosa.a series of fungal cases in hatchery-reared juvenile and young adult siberian sturgeon acipenser baerii and white sturgeon a. transmontanus occurred at production facilities in florida and california, usa, respectively. affected fish exhibited abnormal orientation and/or buoyancy, emaciation, coelomic distension, exophthalmos, cutaneous erythema, and ulcerative skin and eye lesions. necropsies revealed haemorrhage throughout the coelom, serosanguinous coelomic effusion and organomegaly with nodu ...201425320035
electrical excitability of the heart in a chondrostei fish, the siberian sturgeon (acipenser baerii).sturgeon (family acipenseridae) are regarded as living fossils due to their ancient origin and exceptionally slow evolution. to extend our knowledge of fish cardiac excitability to a chondrostei fish, we examined electrophysiological phenotype of the siberian sturgeon (acipenser baerii) heart with recordings of epicardial ecg, intracellular action potentials (aps), and sarcolemmal ion currents. epicardial ecg of a. baerii had the typical waveform of the vertebrate ecg with q-t interval (average ...201425163915
establishment condition and characterization of heart-derived cell culture in siberian sturgeon (acipenser baerii).this study was conducted to establish the efficient condition for stable derivation of heart-derived cell culture in siberian sturgeon (acipenser baerii). three factors including isolation methods, cell densities in initial seeding, and basal media were evaluated for the derivation of heart-derived cell culture. as the results, enzymatic isolation was more efficient than mechanical isolation in both cell retrieval and further culture. total 48 trials of culture employing low and middle cell dens ...201425052194
siberian sturgeon oocyte extract induces epigenetic modifications of porcine somatic cells and improves developmental competence of scnt embryos.somatic cell nuclear transfer (scnt) has generally demonstrated that a differentiated cell can convert into a undifferentiated or pluripotent state. in the scnt experiment, nuclear reprogramming is induced by exposure of introduced donor nuclei to the recipient cytoplasm of matured oocytes. however, because the efficiency of scnt still remains low, a combination of scnt technique with the ex-ovo method may improve the normal development of scnt embryos. here we hypothesized that treatment of som ...201425049951
fatty acid composition in tissues of the farmed siberian sturgeon (acipenser baerii).the fatty acid (fa) compositions of the diet and diverse tissues of the farmed siberian sturgeon (acipenser baerii) were analyzed in detail to assess their nutritional quality. twelve male fish were sampled for muscle, fat, liver, brain, gill, kidney and gonad and the tissue fa measured by gas-liquid chromatography. the fa profile of the diet diverged from the fa signatures of the tissues, where the sturgeons accumulated particular highly-unsaturated fa (hufa). they were probably derived from th ...201424767029
variations of some physiological and immunological parameters in siberian sturgeon (acipenser baerii, brandt, 1869) subjected to an acute stressor.this study was carried out to investigate the effect of an acute stressor on the variation of some physiological and immunological parameters of siberian sturgeon (acipenser baerii) juveniles. fish, reared in 3 tanks for 10 weeks, were used for this study. the acute stress of fish consisted of 2 min of air exposure stress. plasma levels of cortisol, glucose, and lactate as well as lysozyme activity in plasma were measured before stress and 1 hr, 3 hr, 6 hr, 9 hr, 12 hr, and 24 hr after stress. t ...201424484309
3-d structure, volume, and dna content of erythrocyte nuclei of polyploid fish.we have explored the potential relationship between ploidy level, dna content (pg dna nucleus(-1)), and dimensional characteristics, such as volume (μm(3)), surface area (μm(2)), and 3-d structure of erythrocyte nuclei in a series of fish ploidy level models using feulgen image analysis densitometry, flow cytometry, and confocal laser scanning microscopy. the species were diploid tench (tinca tinca) (2n), cuban gar (atractosteus tristoechus) (2n), triploid tench (3n), evolutionary tetraploid ste ...201424446105
inhibition of β-n-acetylglucosaminidase by acetamide affects sperm motility and fertilization success of rainbow trout (oncorhynchus mykiss) and siberian sturgeon (acipenser baerii).β-n-acetylglucosaminidase (β-nagase) is an enzyme found in the sperm acrosome of numerous animal species including fish. fish spermatozoa differ in their morphology including acrosome or acrosomeless aquasperm in chondrostean (e.g., sturgeon) and teleostean (e.g., rainbow trout). it has been shown that β-nagase exists with high activity in both eggs and sperm of these species. the present study shows the potency of β-nagase in fertilization. in rainbow trout, increase in sperm motility parameter ...201424433780
fertility of a spontaneous hexaploid male siberian sturgeon, acipenser baerii.evolution of sturgeons and paddlefishes (order acipenseriformes) is inherently connected with polyploidization events which resulted in differentiation of ploidy levels and chromosome numbers of present acipenseriform species. moreover, allopolyploidization as well as autopolyploidization seems to be an ongoing process in these fishes and individuals with abnormal ploidy levels were occasionally observed within sturgeon populations. here, we reported occurrence of siberian sturgeon (acipenser ba ...201424410899
intersex gonad differentiation in cultured russian (acipenser gueldenstaedtii) and siberian (acipenser baerii) sturgeon.among sturgeons, the occurrence of individuals with gonads containing both testis and ovary components is considered pathological, and such fish are described as intersex individuals or intersexes. intersexes are observed in both wild and cultured populations of sturgeon, usually at low frequencies. in the present study, intersex russian (acipenser gueldenstaedtii) and siberian (acipenser baerii) sturgeons constituted 30% of the studied populations. macroscopically, intersex gonads were recogniz ...201424403549
induction of meiotic gynogenesis in ship sturgeon acipenser nudiventris using uv-irradiated heterologous sperm.diploid gynogenesis was induced in ship sturgeon acipenser nudiventris using uv-irradiated sperm from siberian sturgeon acipenser baerii. the optimal condition for the retention of the second polar body in ship sturgeon was determined to be 10 min after activation/fertilization in experiments. the temperature of cold shock and its duration were 2.5 °c and 30 min, respectively. a total of 30 gynogens of known parentage from experimental treatments were screened using microsatellite dna analysis, ...201424338125
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