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effect of piper betle and brucea javanica on the differential expression of hyphal wall protein (hwp1) in non-candida albicans candida (ncac) species.the study aimed to identify the hwp1 gene in non-candida albicans candida species and the differential expression of hwp1 following treatment with piper betle and brucea javanica aqueous extracts. all candidal suspensions were standardized to 1 × 10(6) cells/ml. the suspension was incubated overnight at 37 °c (c. parapsilosis, 35°c). candidal cells were treated with each respective extract at 1, 3, and 6 mg/ml for 24 h. the total rna was extracted and reverse transcription-polymerase chain react ...201323853657
an in vitro study on the anti-adherence effect of brucea javanica and piper betle extracts towards oral candida.the adherence of candida to mucosal surfaces is the initial step for successful invasive process of the oral cavity. the study aimed to investigate the effect of two plant extracts on the non-specific and specific bindings of oral candida.201323915676
pediculicidal effect of herbal shampoo against pediculus humanus capitis in vitro.human head lice infestation is an important public health problem in thailand. lice resistance is increasing, chemical pediculicides have lost their efficacy and thus alternative products such as herbal shampoos have been proposed to treat lice infestation. the present study investigated the efficacy of twenty nine herbal shampoos based on zinbiberaceae plants, piperaceae plants and native plants against human head lice and compared them with malathion shampoo (a-lices shampoo®: 1% w/v malathion ...201323959497
antioxidant and cytoprotective activities of piper betle, areca catechu, uncaria gambir and betel quid with and without calcium hydroxide.betel quid chewing is a popular habit in southeast asia. it is believed that chewing betel quid could reduce stress, strengthen teeth and maintain oral hygiene. the aim of this study was to investigate the antioxidant and cytoprotective activities of each of the ingredients of betel quid and compared with betel quid itself (with and without calcium hydroxide). the correlation of their cytoprotective and antioxidant activities with phenolic content was also determined.201324330738
growth inhibitory response and ultrastructural modification of oral-associated candidal reference strains (atcc) by piper betle l. extract.candida species have been associated with the emergence of strains resistant to selected antifungal agents. plant products have been used traditionally as alternative medicine to ease mucosal fungal infections. this study aimed to investigate the effects of piper betle extract on the growth profile and the ultrastructure of commonly isolated oral candidal cells. the major component of p. betle was identified using liquid chromatography-mass spectrophotometry (lc-ms/ms). seven atcc control strain ...201424406634
inhibitory effect of piper betle linn. leaf extract on protein glycation--quantification and characterization of the antiglycation components.piper betle linn. is a pan-asiatic plant having several beneficial properties. protein glycation and advanced glycation end products (ages) formation are associated with different pathophysiological conditions, including diabetes mellitus. our study aims to find the effect of methanolic extract of p. betle leaves on in vitro protein glycation in bovine serum albumin (bsa)-glucose model. the extract inhibits glucose-induced glycation, thiol group modification and carbonyl formation in bsa in dose ...201324772978
screening and study of antifungal activity of leaf litter actinomycetes isolated from ternate island, indonesia.to characterize abundance of leaf litter actinomycetes from ternate island and to assess the antifungal activity of actinomycetes isolates against candida albicans, saccharomyces cerevisiae (s. cerevisiae), and aspergillus niger.201425312129
inhibition of aflatoxin biosynthesis in aspergillus flavus by phenolic compounds extracted of piper betle l.food contamination by aflatoxins is an important food safety concern for agricultural products. in order to identify and develop novel antifungal agents, several plant extracts and isolated compounds have been evaluated for their bioactivities. anti-infectious activity of piper betle used in traditional medicine of malaysia has been reported previously.201325848517
effect of aqueous extracts of mentha arvensis (mint) and piper betle (betel) on growth and citrinin production from toxigenic penicillium citrinum.due to growing concern of consumers about chemical residues in food products, the demand for safe and natural food is increasing greatly. the use of natural additives such as spices and herbal oil as seasoning agents for their antimicrobial activity has been extensively investigated. this paper discusses the efficacy of the aqueous extract of mint (mentha arvensis) and betel (piper betle) on the mycelial growth and citrinin production of penicillium citrinum. the present investigation revealed t ...201526028728
piper betle-mediated synthesis, characterization, antibacterial and rat splenocyte cytotoxic effects of copper oxide nanoparticles.the study reports a simple, inexpensive, and eco-friendly synthesis of copper oxide nanoparticles (cuonps) using piper betle leaf extract. formation of cuonps was confirmed by uv-visible spectroscopy at 280 nm. transmission electron microscopy (tem) images showed that the cuonps were spherical, with an average size of 50-100 nm. the scanning electron microscopy (sem)-energy dispersive spectroscopy (eds) peak was observed approximately at 1 and 8 kev. the x-ray diffraction (xrd) studies indicated ...201626148178
modelling the effect of essential oil of betel leaf (piper betle l.) on germination, growth, and apparent lag time of penicillium expansum on semi-synthetic media.the current study aimed at characterizing the chemical components of betel leaf (piper betle l. var. tamluk mitha) essential oil (bleo) and modelling its effect on growth of penicillium expansum on semi-synthetic medium. gas chromatography-mass spectrophotometry (gc-ms) analysis of bleo revealed the presence of different bioactive phenolic compounds in significant amounts. among 46 different components identified, chavibetol (22.0%), estragole (15.8%), β-cubebene (13.6%), chavicol (11.8%), and c ...201526439423
antimicrobial activities of methanol, ethanol and supercritical co2 extracts of philippine piper betle l. on clinical isolates of gram positive and gram negative bacteria with transferable multiple drug resistance.piper betle l. has traditionally been used in alternative medicine in different countries for various therapeutic purposes, including as an anti-infective agent. however, studies reported in the literature are mainly on its activities on drug susceptible bacterial strains. this study determined the antimicrobial activities of its ethanol, methanol, and supercritical co2 extracts on clinical isolates of multiple drug resistant bacteria which have been identified by the infectious disease society ...201626741962
in vitro susceptibilities of wild and drug resistant leishmania donovani amastigotes to piperolactam a loaded hydroxypropyl-β-cyclodextrin nanoparticles.leishmaniasis is an epidemic in various countries, and the parasite leishmania donovani is developing resistance against available drugs. in the present study the antileishmanial action of piperolactam a (pl), isolated after bioactivity guided fractionation from root extracts of piper betle was accentuated in detail. activity potentiation was achieved via cyclodextrin complexation. crude hydro-ethanolic extract (pb) and three fractions obtained from pb and fabricated pl-hydroxypropyl-β-cyclodext ...201626940000
effects of extracts from thai piperaceae plants against infection with toxoplasma gondii.herbal medicines and natural herb extracts are widely used as alternative treatments for various parasitic diseases, and such extracts may also have potential to decrease the side effects of the standard regimen drugs used to treat toxoplasmosis (sulfadiazine-pyrimethamine combination). we evaluated how effective the thai piperaceae plants piper betle, p. nigrum and p. sarmentosum are against toxoplasma gondii infection in vitro and in vivo. individually, we extracted the piperaceae plants with ...201627213575
thin layer chromatography-bioautography and gas chromatography-mass spectrometry of antimicrobial leaf extracts from philippine piper betle l. against multidrug-resistant bacteria.this study isolated and identified the antimicrobial compounds of philippine piper betle l. leaf ethanol extracts by thin layer chromatography- (tlc-) bioautography and gas chromatography-mass spectrometry (gc-ms). initially, tlc separation of the leaf ethanol extracts provided a maximum of eight compounds with r f values of 0.92, 0.86, 0.76, 0.53, 0.40, 0.25, 0.13, and 0.013, best visualized when inspected under uv 366 nm. agar-overlay bioautography of the isolated compounds demonstrated two sp ...201627478476
antibacterial activity of polyaniline coated silver nanoparticles synthesized from piper betle leaves extract.plants or natural resources have been found to be a good alternative method for nanoparticles synthesis. in this study, polyaniline coated silver nanoparticles (agnps) synthesized from piper betle leaves extract were investigated for their antibacterial activity. silver nanoparticles were prepared from the reduction of silver nitrate and nabh4 was used as reducing agent. silver nanoparticles and extracts were mixed thoroughly and then coated by polyaniline. prepared nanoparticles were characteri ...201627642330
piper betle and its bioactive metabolite phytol mitigates quorum sensing mediated virulence factors and biofilm of nosocomial pathogen serratia marcescens in vitro.piper betle, a tropical creeper plant belongs to the family piperaceae. the leaves of this plant have been well known for their therapeutic, religious and ceremonial value in south and southeast asia. it has also been reported to possess several biological activities including antimicrobial, antioxidant, antinociceptive, antidiabetic, insecticidal and gastroprotective activities and used as a common ingredient in indigenous medicines. in indian system of ayurvedic medicine, p. betle has been wel ...201627721053
killing of staphylococcus aureus by allylpyrocatechol is potentiated by induction of intracellular oxidative stress and inhibition of catalase activity.this study investigated the effects of allylpyrocatechol (apc), the major component in ethanolic extract of piper betle, on key oxidative stress resistance enzymes important for the survival of staphylococcus aureus, a major pathogen in the human host.201627854197
allylpyrocatechol attenuates collagen-induced arthritis via attenuation of oxidative stress secondary to modulation of the mapk, jak/stat, and nrf2/ho-1 pathways.rheumatoid arthritis (ra), an inflammatory autoimmune disorder, is characterized by synovial hyperplasia and bony destruction. the pathogenesis of ra includes redox dysregulation, concomitant with increased levels of proinflammatory mediators. as the ability of allylpyrocatechol (apc), a phytoconstituent of piper betle leaves, to alleviate oxidative stress has been demonstrated in patients with ra, its antiarthritic activity was evaluated in an animal model of arthritis, and the underlying mecha ...201727856937
effects of thai piperaceae plant extracts on neospora caninum infection.neosporosis has a worldwide distribution and causes economic losses in farming, particularly by increasing the risk of abortion in cattle. this study investigated the effects of thai piperaceae (piper betle, p. nigrum, and p. sarmentosum) extracts on neospora caninum infections in vitro and in vivo. in an in vitro parasite growth assay based on the green fluorescent protein (gfp) signal, p. betle was the most effective extract at inhibiting parasite growth in human foreskin fibroblast cells (ic5 ...201728137670
comparative analysis of mosquito (diptera: culicidae: aedes aegypti liston) responses to the insecticide temephos and plant derived essential oil derived from piper betle l.resistance to treatments with temephos or plant derived oil, pb-cvo, between a field collected wild strain (ws) and a susceptible laboratory strain (ls) of ae. aegypti were measured. the temephos (0.1mg/l) showed the greatest percentage of mosquito mortality compared to pb-cvo (1.5mg/l) in ls ae. aegypti. however, ws ae. aegypti was not significantly affected by temephos (0.1mg/l) treatment compare to the pb-cvo (1.5mg/l). however, both strains (ls and ws) when treated with pb-cvo (1.5mg/l) disp ...201728213320
piper betle leaf extract affects the quorum sensing and hence virulence of pseudomonas aeruginosa pao1.quorum sensing (qs) plays an important role in virulence of pseudomonas aeruginosa, blocking of qs ability are viewed as viable antimicrobial chemotherapy and which may prove to be a safe anti-virulent drug. bioactive components from piper betle have been reported to possess antimicrobial ability. this study envisages on the anti-qs properties of ethanolic extract of p. betle leaf (pble) using p. aeruginosa pao1 as a model organism. a marked reduction in swarming, swimming, and twitching ability ...201628330090
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