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chemical compositions and antioxidant properties of essential oils from nine species of turkish plants obtained by supercritical carbon dioxide extraction and steam distillation.chemical compositions and antioxidant activities of essential oils from nine different species of turkish plants, namely melissa officinalis l., rosmarinus officinalis l., cuminum cyminum l., piper nigrum l., lavandula stoechas spp., foeniculum vulgare, pimpinella anisum l., thymus serpyllum and liquidamber orientalis mill., were studied. essential oils were obtained by supercritical carbon dioxide (scco2) extraction and steam distillation, and were analyzed by gas chromatography-mass spectromet ...200819382349
isolation and amplification of genomic dna from recalcitrant dried berries of black pepper (piper nigrum l.)--a medicinal spice.black pepper is an important medicinal spice traded internationally. the extraction of high quality genomic dna for pcr amplification from dried black pepper is challenging because of the presence of the exceptionally large amount of oxidized polyphenolic compounds, polysaccharides and other secondary metabolites. here we report a modified hexadecyl trimethyl ammonium bromide (ctab) protocol by incorporating potassium acetate and a final peg precipitation step to isolate pcr amplifiable genomic ...200717914177
larvicidal effects of crude extracts of dried ripened fruits of piper nigrum against culex quinquefasciatus larval instars. 200919502696
effect of spices on lipid metabolism in 1,2-dimethylhydrazine-induced rat colon carcinogenesis.colon cancer is the second most common cancer among men and women worldwide. we investigated the effect of red chilli (capsicum annum l.), cumin (cuminum cyminum l.), and black pepper (piper nigrum l.) on colon cancer induced in rats by a colon-specific carcinogen, 1,2-dimethylhydrazine (dmh). colon cancer was induced by subcutaneous injection of dmh at a dosage of 20 mg/kg of body weight (15 doses, at 1-week intervals). the rats were continued with the standard pellet diet and supplemented red ...200616822210
alkaloids from piper sarmentosum and piper nigrum.detailed chemical studies on the roots of piper sarmentosum and piper nigrum have resulted in several alkaloids. the roots of p. sarmentosum gave a new aromatic compound, 1-nitrosoimino-2,4,5-trimethoxybenzene (1). piper nigrum roots gave pellitorine (2), (e)-1-[3',4'-(methylenedioxy)cinnamoyl]piperidine (3), 2,4-tetradecadienoic acid isobutyl amide (4), piperine (5), sylvamide (6), cepharadione a (7), piperolactam d (8) and paprazine (9). structural elucidation of these compounds was achieved t ...200919809914
bactericidal activity of black pepper, bay leaf, aniseed and coriander against oral isolates.present investigation focused on antibacterial potential of aqueous decoction of black pepper (piper nigrum l.), bay leaf (laurus nobilis l.), aniseed (pimpinella anisum l.), and coriander (coriandum sativum l.) against 176 bacterial isolates belonging to 12 different genera of bacterial population isolated from oral cavity of 200 individuals. the disc diffusion technique was employed. overall aqueous decoction of black pepper was the most bacterial-toxic exhibited 75% antibacterial activity as ...200616935829
a rapid method for isolation of piperine from the fruits of piper nigrum linn.a simple, rapid and efficient method has been developed for the isolation of piperine from the fruits of piper nigrum. the method involves extraction of the fruit powder with glacial acetic acid, from which piperine is partitioned into chloroform and subsequently crystallized. the identity of the compound was confirmed by its melting point, comparison of uv, ir, and mass spectral data with those from a reference standard, and co-chromatography with the reference standard using thin-layer chromat ...200818404304
molecular target of piperine in the inhibition of lipid droplet accumulation in macrophages.an alkaloid piperine isolated from the piper nigrum was found to inhibit lipid droplet accumulation in mouse macrophages, and especially inhibited cholesteryl ester (ce) synthesis (ic50: 25 microm). the metabolism of cholesterol from lysosome to lipid droplet was inhibited with a similar ic50 (18 microm), indicating that the site of inhibition is one of the steps between the lysosomes and the endoplasmic reticulum. therefore, effects of piperine on acyl-coa:cholesterol acyltransferase (acat) act ...200818520030
safety of a novel botanical extract formula for ameliorating allergic rhinitis.allergic rhinitis (also known as hay fever) is the most commonly occurring immunological disorder, and it affects 40 million men, women, and children in the united states. symptomatically, it is an inflammation and irritation of the mucous membranes that line the nose. allergy is defined as a state of hypersensitivity or hyperimmunity caused by exposure to a particular antigen (allergen) that results in increased reactivity upon subsequent exposure. a novel botanical formulation, aller-7/nr-a2, ...200320021150
binding of bioactive phytochemical piperine with human serum albumin: a spectrofluorometric study.piperine, the bioactive alkaloid compound of the spice black pepper (piper nigrum) exhibits a wide range of beneficial physiological and pharmacological activities. being essentially water-insoluble, piperine is presumed to be assisted by serum albumin for its transport in blood. in this study, the binding of piperine to serum albumin was examined by employing steady state and time resolved fluorescence techniques. binding constant for the interaction of piperine with human serum albumin, which ...200717407131
physicochemical and microbiological qualities of steamed and irradiated ground black pepper (piper nigrum l.).the effects of steam and irradiation treatments on the physicochemical properties (moisture content, ph, extractable yield, reducing sugar, soluble pigment, antioxidant activity, piperine, hunter's color, and sensory attributes) and microbiological quality (total aerobic bacteria, coliforms, and yeasts and molds) of ground black pepper stored at refrigerated and room temperatures for 6 months were compared and evaluated. irradiation resulted in a higher microbial reduction in pepper, with minima ...200818522396
black pepper and its pungent principle-piperine: a review of diverse physiological effects.black pepper (piper nigrum) is one of the most widely used among spices. it is valued for its distinct biting quality attributed to the alkaloid, piperine. black pepper is used not only in human dietaries but also for a variety of other purposes such as medicinal, as a preservative, and in perfumery. many physiological effects of black pepper, its extracts, or its major active principle, piperine, have been reported in recent decades. dietary piperine, by favorably stimulating the digestive enzy ...200717987447
hplc-based activity profiling: discovery of piperine as a positive gaba(a) receptor modulator targeting a benzodiazepine-independent binding site.a plant extract library was screened for gaba(a) receptor activity making use of a two-microelectrode voltage clamp assay on xenopus laevis oocytes. an ethyl acetate extract of black pepper fruits [piper nigrum l. (piperaceae) 100 microg/ml] potentiated gaba-induced chloride currents through gaba(a) receptors (composed of alpha(1), beta(2), and gamma(2s) subunits) by 169.1 +/- 2.4%. with the aid of an hplc-based activity profiling approach, piperine (5) was identified as the main active compound ...201020085307
a new natural product and insecticidal amides from seeds of piper nigrum linn.studies on the petroleum ether soluble and insoluble fraction of ethanol extract of dried ground seeds of piper nigrum resulted in the isolation and structure elucidation of 1 new and 11 known compounds which include 3 hitherto unreported constituents, namely, cinnamylideneacetone, 3,4-methylenedioxyphenylpropiophenone and 2-hydroxy-4,5-methylenedioxypropiophenone from this plant.200818855209
studies on effect of piperine on oral bioavailability of ampicillin and norfloxacin.ampicillin and norfloxacin are used to treat variety of bacterial infections. these two drugs have low oral bioavailability. co-administration of piperine (20 mg/kg), an alkaloid from piper nigrum l. enhanced oral bioavailability of ampicillin and norfloxacin in animal model. this is reflected in various pharmacokinetic measurements like cmax, tmax, auc and t(1/2) of the above antibiotics in animal model.200820161946
amides from piper nigrum l. with dissimilar effects on melanocyte proliferation in-vitro.melanocyte proliferation stimulants are of interest as potential treatments for the depigmentary skin disorder, vitiligo. piper nigrum l. (piperaceae) fruit (black pepper) water extract and its main alkaloid, piperine (1), promote melanocyte proliferation in-vitro. a crude chloroform extract of p. nigrum containing piperine was more stimulatory than an equivalent concentration of the pure compound, suggesting the presence of other active components. piperine (1), guineensine (2), pipericide (3), ...200717430636
pellitorine, a potential anti-cancer lead compound against hl6 and mct-7 cell lines and microbial transformation of piperine from piper nigrum.pellitorine (1), which was isolated from the roots of piper nigrum, showed strong cytotoxic activities against hl60 and mct-7 cell lines. microbial transformation of piperine (2) gave a new compound 5-[3,4-(methylenedioxy)phenyl]-pent-2-ene piperidine (3). two other alkaloids were also found from piper nigrum. they are (e)-1-[3',4'-(methylenedioxy)cinnamoyl]piperidine (4) and 2,4-tetradecadienoic acid isobutyl amide (5). these compounds were isolated using chromatographic methods and their struc ...201020428051
tissue distribution & elimination of capsaicin, piperine & curcumin following oral intake in rats.curcumin, capsaicin and piperine--the bioactive compounds present in spices--turmeric (curcuma longa), red pepper (capsicum annuum) and black pepper (piper nigrum) respectively, have a considerable portion of structural homology. tissue distribution and elimination of these three structurally similar bioactive compounds was examined following their oral intake in rats.201020516541
larvicidal potential of ethanolic extracts of dried fruits of three species of peppercorns against different instars of an indian strain of dengue fever mosquito, aedes aegypti l. (diptera: culicidae).larvicidal bioassay was carried out in the laboratory to assess the potential of ethanolic extracts of dried fruits of three species of peppercorns: long pepper, piper longum l., black pepper, piper nigrum, and white pepper, piper nigrum against the different instars of field-collected indian strain of dengue fever mosquito (aedes aegypti l.). the investigations established the larvicidal potential of all the varieties of pepper fruits against ae. aegypti. against early fourth instar, the ethano ...201020549234
alkamides from the fruits of piper longum and piper nigrum displaying potent cell adhesion inhibition.eight alkamides 1-8 were isolated by bioassay-guided isolation of etoh extracts of the fruits of piper longum and piper nigum (piperaceae). their structures were elucidated by spectroscopic analysis ((1)h, (13)c nmr, and esi-ms) as follows: guineensine (1), retrofracamide c (2), (2e,4z,8e)-n-[9-(3,4-methylenedioxyphenyl)-2,4,8-nonatrienoyl]piperidine (3), pipernonaline (4), piperrolein b (5), piperchabamide d (6), pellitorin (7), and dehydropipernonaline (8). their compounds 3-5, 7, and 8 inhibi ...200818672369
potential mechanisms of hypocholesterolaemic effect of thai spices/dietary extracts.several thai spices/dietary ingredients were previously shown to have hypocholesterolaemic effects. these studies were mostly conducted in animal models in which the mechanisms of action were not yet well-established. therefore, this study aimed to investigate the potential mechanism of hypocholesterolaemic action of 12 selected plants, namely hibiscus sabdariffa l., moringa oleifera lam., cucurbita moschata duchesne, ananas comosus (l.) merr., zingiber officinale, morus alba l., camellia sinens ...201120623425
antispasmodic effect of piper nigrum fruit hot water extract on rat ileum.the aim of this study was to investigate the effect of black pepper fruit hot water extract (bpe) on rat ileum contractility and the mechanism(s) of its action. the extract was prepared by adding black pepper powder to boiling distilled water followed by evaporated the solvent. ileum was dissected from male adult rat (wistar) and in tyrode solution the tissue contractions were recorded by an isotonic transducer under 1 g tension. the cumulative concentrations of the bpe (0.0625-1 mg ml(-1)) redu ...200818817253
histamine release inhibitory activity of piper nigrum leaf.oral administration of a methanolic extract of piper nigrum leaf (pn-ext, 50, 200 and 500 mg/kg) showed a potent dose-dependent inhibition of dinitrofluorobenzene (dnfb)-induced cutaneous reaction at 1 h [immediate phase response (ipr)] after and 24 h [late phase response (lpr)] after dnfb challenge in mice which were passively sensitized with anti-dinitrophenyl (dnp) ige antibody. ear swelling inhibitory effect of pn-ext (50, 200 and 500 mg/kg, per os (p.o.)) on very late phase response (vlpr) ...200818827366
stability-indicating reversed-phase liquid chromatographic methods for the determination of aconitine and piperine in a polyherbal formulation.simple and rapid stability-indicating hplc methods were developed for the individual analysis of aconitine (acn) and piperine (pin) in mahamrutynjaya rasa, an herbal dosage form containing aconitum ferox, piper nigrum, and piper longum in combination. separation of the acn from its major and minor degradation products was successfully achieved on a reversed-phase c18 column (250 x 4.6 mm id, 5 microm particle size), with isocratic elution using a mixture of acetonitrile-kh2po4 buffer (10 mm, ph ...200919714970
characterisation of phytophthora capsici isolates from black pepper in vietnam.phytophthora foot rot of black pepper caused by phytophthora capsici is a major disease of black pepper (piper nigrum) throughout vietnam. to understand the population structure of p. capsici, a large collection of p. capsici isolates from black pepper was studied on the basis of mating type, random amplified microsatellites (rams) and repetitive extragenic palindromic (rep) fingerprinting. two mating types a1 and a2 were detected in four provinces in two climatic regions, with a1:a2 ratios rang ...201020960972
antioxidant and anti-atherogenic activities of three piper species on atherogenic diet fed hamsters.atherogenic diet is known to induce high plasma lipid concentration, oxidative stress and early atherosclerosis. antioxidants have potentials to counter the effect of atherogenic diet. the present research aims at evaluating the antioxidant and anti-atherosclerotic activities of three piper species (piper guineense, piper nigrum and piper umbellatum) on atherogenic diet fed hamsters. hamsters divided into 8 groups: normal control, atherosclerotic control and six test groups. the normal animals f ...201021035316
piperine, an active principle from piper nigrum, modulates hormonal and apo lipoprotein profiles in hyperlipidemic rats.to study the effect of piperine, an alkaloid, on thyroid hormones and apolipoproteins in high-fat-diet (hfd) and antithyroid drug-induced hyperlipidemic rats.200616910313
fumigant toxicity of essential oils from some common spices against pulse beetle, callosobruchus chinensis (coleoptera: bruchidae).in the present study, the essential oil from seven common spices, anethum graveolens, cuminum cyminum, illicium verum, myristica fragrans, nigella sativa, piper nigrum and trachyspermum ammi was isolated and its insecticidal, oviposition, egg hatching and developmental inhibitory activities were determined against pulse beetle, callosobruchus chinensis. essential oils were isolated by hydrodistillation method using clevenger apparatus. these essential oils caused death of adults and larvae of ca ...200818270466
in vitro investigation of the potential immunomodulatory and anti-cancer activities of black pepper (piper nigrum) and cardamom (elettaria cardamomum).although the immunomodulatory effects of many herbs have been extensively studied, research related to possible immunomodulatory effects of various spices is relatively scarce. here, the potential immunomodulatory effects of black pepper and cardamom are investigated. our data show that black pepper and cardamom aqueous extracts significantly enhance splenocyte proliferation in a dose-dependent, synergistic fashion. enzyme-linked immunosorbent assay experiments reveal that black pepper and carda ...201020210607
uv irradiation affects melanocyte stimulatory activity and protein binding of piperine.piperine, the major alkaloid of black pepper (piper nigrum l.; piperaceae), stimulates melanocyte proliferation and dendrite formation in vitro. this property renders it a potential treatment for the skin depigmentation disorder vitiligo. however, piperine does not stimulate melanin synthesis in vitro, and treatments based on this compound may therefore be more effective with concomitant exposure of the skin to ultraviolet (uv) radiation or sunlight. the present study investigated the effect of ...200617387768
analysis of aromatic and terpenic constituents of pepper extracts by capillary electrochromatography.an original method based on cec has been developed for the determination of aromatic and terpenic compounds in extracts of spices obtained from piper nigrum. the method is based on the use of a fused silica capillary (effective length: 23.5 cm, internal diameter: 100 microm) packed with a c18 sorbent (packing length: 23 cm, particle size: 5 microm). the mobile phase is a 50 mm, ph 6.0 ammonium acetate/acn (10:90 v/v) mixture. applying a 30 kv voltage, the following 11 compounds were separated an ...200717444231
studies on the effects of phosphine on salmonella enterica serotype enteritidis in culture medium and in black pepper (piper nigrum).the effect of phosphine on salmonella enterica serotype enteritidis inoculated in culture medium and in black pepper grains (piper nigrum), as well as on the reduction of the microbial load of the dried and moisturized product, was verified. the postfumigation effect was verified in inoculated samples with 0.92 and 0.97 water activity (a(w)) exposed to 6 g/m(3) phosphine for 72 h, dried to 0.67 a(w), and stored for 24, 48, and 72 h. no decreases were observed in salmonella enteritidis population ...201121477485
characterization and control of endophytic bacterial contaminants in in vitro cultures of piper spp., taxus baccata subsp. wallichiana, and withania somnifera.bacterial contamination is a serious problem that causes severe loss of in vitro grown cultures of a number of plants. this problem becomes even more acute if the bacterial contamination is of endophytic origin. in such cases, identification and characterization of the contaminants is essential for achieving specific control of the contaminants through selective use of antibiotic agents, especially if the routinely used contamination control methods practiced elsewhere in tissue culture studies ...200717496951
the plant alkaloid piperine as a potential inhibitor of ethidium bromide efflux in mycobacterium smegmatis.piperine, a major plant alkaloid found in black pepper (piper nigrum) and long pepper (piper longum), has shown potential for inhibiting the efflux pump (ep) of staphylococcus aureus. in this study, a modulation assay showed that piperine could decrease the mic of ethidium bromide (etbr) twofold at 32 μg ml(-1) and fourfold at 64 μg ml(-1) against mycobacterium smegmatis mc(2) 155 atcc 700084. a real-time, 96-well plate fluorometric method was employed to evaluate the ep inhibition ...201021051548
inhibition of chemiluminescence and chemotactic activity of phagocytes in vitro by the extracts of selected medicinal plants.the methanol extracts of 20 selected medicinal plants were investigated for their effects on the respiratory burst of human whole blood, isolated human polymorphonuclear leukocytes (pmns) and isolated mice macrophages using a luminol/lucigenin-based chemiluminescence assay. we also tested the effect of the extracts on chemotactic migration of pmns using the boyden chamber technique. the extracts of curcuma domestica l., phyllanthus amarus schum & thonn and c. xanthorrhiza roxb. were the samples ...201021184195
antibacterial activity of natural spices on multiple drug resistant escherichia coli isolated from drinking water, bangladesh.spices traditionally have been used as coloring agents, flavoring agents, preservatives, food additives and medicine in bangladesh. the present work aimed to find out the antimicrobial activity of natural spices on multi-drug resistant escherichia coli isolates.201121406097
piperine, the main alkaloid of thai black pepper, protects against neurodegeneration and cognitive impairment in animal model of cognitive deficit like condition of alzheimer's disease.recently, numerous medicinal plants possessing profound central nervous system effects and antioxidant activity have received much attention as food supplement to improve cognitive function against cognitive deficit condition including in alzheimer's disease condition. based on this information, the effect of piperine, a main active alkaloid in fruit of piper nigrum, on memory performance and neurodegeneration in animal model of alzheimer's disease have been investigated. adult male wistar rats ...201020034530
flash chromatography on cartridges for the separation of plant extracts: rules for the selection of chromatographic conditions and comparison with medium pressure liquid chromatography.empirical rules for the selection of chromatographic conditions on flash chromatography cartridges were developed, with an emphasis on gradient mode. these rules were then tested with separation of extracts from important medicinal plants including curcuma xanthorrhiza, piper nigrum and salvia milthiorrhiza. sepacore® cartridges enabled a good separation of compounds with a broad range of polarity, as typically found in plant extracts. the chromatographic resolution remained, however, lower than ...201120804823
piperine, the potential functional food for mood and cognitive disorders.the effect of piperine, the main alkaloid from piper nigrum, on the central nervous system is not clearly known until now. in the present study, male wistar rats were administered piperine at various doses ranging from 5, 10 and 20mg/kg bw once daily for 4 weeks and the animals were determined the neuropharmacological activity after single, 1, 2, 3 and 4 weeks of treatment. the results showed that piperine at all dosage range used in this study possessed anti-depression like activity and cogniti ...200818639606
piperine, a piperidine alkaloid from piper nigrum re-sensitizes p-gp, mrp1 and bcrp dependent multidrug resistant cancer cells.over-expression of p-gp, mrp1 and bcrp in tumor cells is one of the important mechanisms leading to multidrug resistance (mdr), which impairs the efficacy of chemotherapy. p-gp, mrp1 and bcrp are abc (atp-binding cassette) transporters, which can expel a variety of lipophilic anti-cancer drugs and protect tumor cells. during a screening of mdr reversal agents among alkaloids of various structural types, a piperidine alkaloid, piperine (a main piperidine alkaloid in piper nigurm) was identified a ...201121802927
documentation and quantitative analysis of the local knowledge on medicinal plants among traditional siddha healers in virudhunagar district of tamil nadu, india.india has a population with high degree of medical pluralism. siddha system of indian traditional medicine is practiced dominantly by the people in tamil nadu. the traditionally trained siddha healers still play an important role in the rural health care. their knowledge is comparatively more vulnerable than the documented traditional knowledge. thus, the present study was aimed to document and quantitatively analyze the local knowledge of the traditional siddha healers in virudhunagar district ...201121718779
chemistry and in vitro antioxidant activity of volatile oil and oleoresins of black pepper (piper nigrum).essential oil and oleoresins (ethanol and ethyl acetate) of piper nigrum were extracted by using clevenger and soxhlet apparatus, respectively. gc-ms analysis of pepper essential oil showed the presence of 54 components representing about 96.6% of the total weight. beta-caryophylline (29.9%) was found as the major component along with limonene (13.2%), beta-pinene (7.9%), sabinene (5.9%), and several other minor components. the major component of both ethanol and ethyl acetate oleoresins was fou ...200919456163
mediation of cholino-piperine like receptors by extracts of piper nigrum induces melanin dispersion in rana tigerina tadpole melanophores.the present study was carried out to determine the effects of lyophilized dried fruit extracts of piper nigrum and pure piperine on the tadpole melanophores of frog rana tigerina which offer excellent in vitro opportunities for studying the effects of pharmacological and pharmaceutical agents. the nature of specific cellular receptors present on the neuro-melanophore junction and their involvement in pigmentary responses has been explored.201121663558
simultaneous determination of bioactive compounds in piper nigrum l. and a species comparison study using hplc-pda.piper nigrum l. is a traditional medicine widely used in india for illnesses such as constipation, diarrhoea, earache, gangrene, heart disease, hernia, hoarseness, indigestion, insect bites, insomnia, joint pain, liver problems, lung disease, oral abscesses, sunburn, tooth decay and toothaches. in this study, six bioactive compounds, namely piperine (1), pellitorine (2), guineensine (3), pipnoohine (4), trichostachine (5) and piperonal (6) were quantified in different extracts of p. nigrum l. an ...201121854175
adsorption and removal kinetics of phosphonate from water using natural adsorbents.the removal of phosphonate from water was studied using some natural adsorbents. potassium phosphonate is a fungicide used for the control of phytophthora capsici, which is prevalent in black pepper (piper nigrum l.). batch adsorption kinetic experiments were conducted on the adsorption of phosphonate onto the adsorbents. the concentration of phosphonate was measured on a high-performance liquid chromatograph fitted with a conductivity detector. the percentage removal of phosphonate by powdered ...201020112539
larvicidal and repellent activity of medicinal plant extracts from eastern ghats of south india against malaria and filariasis vectors.to evaluate the larvicidal and repellent activities of ethyl acetate and methanol extracts of acacia concinna (a. concinna), cassia siamea (c. siamea), coriandrum sativum (c. sativum),cuminum cyminum (c. cyminum), lantana camara (l. camara), nelumbo nucifera (n. nucifera) phyllanthus amarus (p. amarus), piper nigrum (p. nigrum) and trachyspermum ammi (t. ammi) against anopheles stephensi (an. stephensi) and culex quinquefasciatus (cx. quinquefasciatus).201121967692
Studies on antimicrobial activities of solvent extracts of different spices.The antimicrobial activities of the ethyl acetate, acetone and methanol extract of 12 plant species were studied. The extract of Capsicum annuum (red pepper) (fruit) Zingiber officinale (ginger) (root), Cuminum cyminum (cumin), Alpinia ficinarum (galingale), Coriandrum sativum (coriander), Cinnamomun zeylanicum Nees (cinnamomun), Origanum onites L. (thyme), Folium sennae (senna), Eugenia caryophyllata (cloves), Flos tiliae (lime), Folium menthae crispae (peppermint) and Piper nigrum (blackpepper ...201121882663
acetylcholinesterase inhibitory activity of thai traditional nootropic remedy and its herbal ingredients.the incidence of alzheimer disease (ad) is increasing every year in accordance with the increasing of elderly population and could pose significant health problems in the future. the use of medicinal plants as an alternative prevention or even for a possible treatment of the ad is, therefore, becoming an interesting research issue. acetylcholinesterase (ache) inhibitors are well-known drugs commonly used in the treatment of ad. the aim of the present study was to screen for ache inhibitory activ ...201122619927
medico - botanical study of yercaud hills in the eastern ghats of tamil nadu, india.the study reports medicinal plant survey was conceded in yercaud hills ranges of eastern ghats, tamil nadu, india. the study primarily based on field surveys conducted throughout the hills, where dwellers provided information on plant species used as medicine, plant parts used to prepare the remedies and ailments to which the remedies were prescribed. the study resulted about 48- plant species belonging to 45- genera and 29- families of medicinal plants related to folk medicine used by the local ...201122557438
testosterone 5alpha-reductase inhibitory active constituents of piper nigrum leaf.previously we reported that piper nigrum leaf extract showed a potent stimulation effect on melanogenesis and that (-)-cubebin (1) and (-)-3,4-dimethoxy-3,4-desmethylenedioxycubebin (2) were isolated as active constituents. as a part of our continuous studies on piper species for the development of cosmetic hair-care agents, testosterone 5alpha-reductase inhibitory activity of aqueous ethanolic extracts obtained from several different parts of six piper species, namely piper nigrum, p. methystic ...200718057734
antispermatogenic and antifertility effects of fruits of piper nigrum l. in mice.effect of oral administration (25 and 100 mg/kg body wt/day, for 20 and 90 days) of fruit powder of piper nigrum l. on the male reproductive organs of mice, parkes strain, was investigated. various reproductive end points such as organs weight, histopathology, sperm parameters, sialic acid and fructose contents, and fertility indices were assessed. histologically, testes in treated mice, except in those treated with 100 mg of dose for 90 days, showed non-uniform degenerative changes in the semin ...200919957882
piperine ameliorates the severity of cerulein-induced acute pancreatitis by inhibiting the activation of mitogen activated protein kinases.piperine is a phenolic component of black pepper (piper nigrum) and long pepper (piper longum), fruits used in traditional asian medicine. our previous study showed that piperine inhibits lipopolysaccharide-induced inflammatory responses. in this study, we investigated whether piperine reduces the severity of cerulein-induced acute pancreatitis (ap). administration of piperine reduced histologic damage and myeloperoxidase (mpo) activity in the pancreas and ameliorated many of the examined labora ...201121663734
identifying sequences potentially related to resistance response of piper tuberculatum to fusarium solani f. sp. piperis by suppression subtractive hybridization.piper tuberculatum is an exotic piper from the amazon region that shows resistance to infection by fusarium solani f. sp. piperis, causal agent of fusarium disease in black pepper (piper nigrum l.). in this work we aimed to study the interaction between p. tuberculatum and f. solani f. sp. piperis at a molecular level, using suppression subtractive hybridization to identify genes potentially related to fusarium disease resistance. comparative sequence analysis confirmed that clones isolated here ...200920001904
piper nigrum, piper betle and gnetum gnemon--natural food sources with anti-quorum sensing properties.various parts of piper nigrum, piper betle and gnetum gnemon are used as food sources by malaysians. the purpose of this study is to examine the anti-quorum sensing (anti-qs) properties of p. nigrum, p. betle and g. gnemon extracts. the hexane, chloroform and methanol extracts of these plants were assessed in bioassays involving pseudomonas aeruginosa pa01, escherichia coli [psb401], e. coli [psb1075] and chromobacterium violaceum cv026. it was found that the extracts of these three plants have ...201323519352
piper nigrum leaf and stem assisted green synthesis of silver nanoparticles and evaluation of its antibacterial activity against agricultural plant pathogens.utilization of biological materials in synthesis of nanoparticles is one of the hottest topics in modern nanoscience and nanotechnology. in the present investigation, the silver nanoparticles were synthesized by using the leaf and stem extract of piper nigrum. the synthesized nanoparticle was characterized by uv-vis spectroscopy, x-ray diffraction (xrd), scanning electron microscope (sem), transmission electron microscope (tem), energy dispersive x-ray analysis (edax), and fourier transform infr ...201424558336
potent α-amylase inhibitory activity of indian ayurvedic medicinal plants.indian medicinal plants used in the ayurvedic traditional system to treat diabetes are a valuable source of novel anti-diabetic agents. pancreatic α-amylase inhibitors offer an effective strategy to lower the levels of post-prandial hyperglycemia via control of starch breakdown. in this study, seventeen indian medicinal plants with known hypoglycemic properties were subjected to sequential solvent extraction and tested for α-amylase inhibition, in order to assess and evaluate their inhibitory po ...201121251279
safety of a novel botanical extract formula for ameliorating allergic rhinitis. part ii.abstract each year more than 50 million americans suffer from allergic rhinitis, which is a state of hypersensitivity or hyperimmunity. basically, allergic rhinitis is symptomatically recognized as the inflammation and irritation of the nasal mucosal membranes; sneezing; stuffy/runny nose; nasal congestion; and itchy; watery, and swollen eyes; and defined as a state of hypersensitivity/ hyperimmunity caused by exposure to a particular allergen (antigen) that results in increased reactivity upon ...200520021083
suppression of phorbol-12-myristate-13-acetate-induced tumor cell invasion by piperine via the inhibition of pkca/erk1/2-dependent matrix metalloproteinase-9 expression.piperine is a major component of black pepper, piper nigrum linn, used widely in traditional medicine. several previous studies reported that piperine possesses various beneficial biological activities including antioxidant, anti-tumor and anti-inflammation properties. in the present study, we investigated the inhibitory effects of piperine on tumor invasion and migration and the possible mechanisms involved using human fibrosarcoma ht-1080 cells. we found that piperine suppresses pma-enhanced m ...201121354279
the larvicidal effects of black pepper (piper nigrum l.) and piperine against insecticide resistant and susceptible strains of anopheles malaria vector mosquitoes.insecticide resistance carries the potential to undermine the efficacy of insecticide based malaria vector control strategies. therefore, there is an urgent need for new insecticidal compounds. black pepper (dried fruit from the vine, piper nigrum), used as a food additive and spice, and its principal alkaloid piperine, have previously been shown to have larvicidal properties. the aim of this study was to investigate the larvicidal effects of ground black pepper and piperine against third and fo ...201627117913
repellency of plant extracts against the legume flower thrips megalurothrips sjostedti (thysanoptera: thripidae).megalurothrips sjostedti trybom is an important pest of cowpea (vigna unguiculata l.) in africa. to propose an alternative to chemical control, the repellency of 24 plant extracts was evaluated against adult female thrips of m. sjostedti in the laboratory. plant extracts in ethanol were separately applied on a filter paper disk in a still air visual cue olfactometer. the results showed highly significant differences in repellency among extract type, concentration and their interactions. we class ...201526463406
in vitro antioxidant activity of pet ether extract of black pepper.to investigate the in vitro antioxidant activity of different fractions (r1, r2 and r3) obtained from pet ether extract of black pepper fruits (piper nigrum linn.)200820040947
in vivo effect of piperine on serum and tissue glycoprotein levels in benzo(a)pyrene induced lung carcinogenesis in swiss albino mice.in recent years, considerable emphasis has been focused on identifying new cancer chemopreventive agents, which could be useful for the human population. piperine is a pure, pungent alkaloid constituent of black and long peppers (piper nigrum and piper longum), that acts as an antioxidant and anticancer agent by its numerous macromolecules associated with them. in the present study, piperine was found to suppress benzo(a)pyrene (b(a)p) induced lung cancer in swiss albino mice. in lung cancer bea ...200615975841
[antioxidant properties of essential oils].by the method of capillary gas-liquid chromatography we studied antioxidant properties and stability during the storage of hexane solutions of 14 individual essential oils from black and white pepper (piper nigrum l.), cardamom (elettaria cardamomum l.), nutmeg (myristica fragrans houtt.), mace (myristica fragrans houtt), juniperberry (juniperus communis l.), seed of fennel (foeniculum vulgare mill., var. dulce thelling), caraway (carvum carvi l.), dry leaves of cinnamon (cinnamomum zeylanicum b ...200920067158
the dynamic microbiota profile during pepper (piper nigrum l.) peeling by solid-state fermentation.white pepper (piper nigrum l.), a well-known spice, is the main pepper processing product in hainan province, china. the solid-state method of fermentation can peel pepper in a highly efficient manner and yield high-quality white pepper. in the present study, we used next-generation sequencing to reveal the dynamic changes in the microbiota during pepper peeling by solid-state fermentation. the results suggested that the inoculated aspergillus niger was dominant throughout the fermentation stage ...201728378160
effect of different in vitro culture extracts of black pepper (piper nigrum l.) on toxic metabolites-producing strains.in the present study, the effect of different in vitro cultures (callus, in vitro shoots) and commercially available peppercorn extract was investigated for its activity against toxic metabolite-producing strains (escherichia coli, pseudomonas aeroginosa, salmonella typhi, bacillus subtilis, bacillus cereus, staphylococcus aureus, and candida albicans). these in vitro cultures were extracted with ethanol, hexane, and chloroform, and the antipathogenic activity was determined by well-diffusion me ...201624193053
Selective molecular sequestration with concurrent natural product functionalization and derivatization: from crude natural product extracts to a single natural product derivative in one step.A resin-bound nitroso compound sequestered a single unexpected component from crude plant seed extracts. Several plants, including Piper nigrum, Eugenia caryophyllata, and Pimenta dioica, were extracted with organic solvent in the presence of a nitroso-containing resin. The nitroso resin selectively sequestered a single compound, ß-caryophyllene, via a chemo- and regioselective ene reaction. The ene product was released from the resin, and proper selection of the solid-phase linker and cleavage ...201122059469
isolation, characterization and identification of pericarp-degrading bacteria for the production of off-odour-free white pepper from fresh berries of piper nigrum l.to isolate, fermentatively evaluate and identify black pepper (piper nigrum l.)-associated bacteria for the microbial decortication of fresh ripened berries and dried black pepper for preparation of off-odour-free white pepper.201424387754
high-frequency plant regeneration through cyclic secondary somatic embryogenesis in black pepper (piper nigrum l.).a high-frequency plantlet regeneration protocol was developed for black pepper (piper nigrum l.) through cyclic secondary somatic embryogenesis. secondary embryos formed from the radicular end of the primary somatic embryos which were originally derived from micropylar tissues of germinating seeds on growth regulator-free sh medium in the absence of light. the process of secondary embryogenesis continued in a cyclic manner from the root pole of newly formed embryos resulting in clumps of somatic ...200616249871
piperine protects cisplatin-induced apoptosis via heme oxygenase-1 induction in auditory cells.piperine is a major component of black pepper, piper nigrum linn, used widely in traditional medicine. in this study, we examined whether piperine could protect house ear institute-organ of corti 1 (hei-oc1) cells against cisplatin-induced apoptosis through the induction of heme oxygenase (ho)-1 expression. piperine (10-100 microm) induced the expression of ho-1 in dose- and time-dependent manners. piperine also induced antioxidant response element-luciferase and translocated nuclear factor-e2-r ...200717418561
inhibition of lipopolysaccharide-induced inflammatory responses by piperine.piperine, a main component of piper longum linn. and piper nigrum linn., is a plant alkaloid with a long history of medical use. piperine exhibits anti-inflammatory activity; however, the underlying mechanism remains unknown. we examined the effects of piperine on lipopolysaccharide (lps)-induced inflammatory responses. administration of piperine inhibited lps-induced endotoxin shock, leukocyte accumulation and the production of tumor necrosis factor-alpha (tnf-alpha), but not of interleukin (il ...201020621590
endophytic bacteria from piper tuberculatum jacq.: isolation, molecular characterization, and in vitro screening for the control of fusarium solani f. sp piperis, the causal agent of root rot disease in black pepper (piper nigrum l.).endophytic bacteria have been found to colonize internal tissues in many different plants, where they can have several beneficial effects, including defense against pathogens. in this study, we aimed to identify endophytic bacteria associated with roots of the tropical piperaceae piper tuberculatum, which is known for its resistance to infection by fusarium solani f. sp piperis, the causal agent of black pepper (piper nigrum) root rot disease in the amazon region. based on 16s rrna gene sequence ...201526214435
immunomodulatory role of piperine in cadmium induced thymic atrophy and splenomegaly in mice.cadmium being a potent immunotoxicant, affects both humoral and cell mediated immunity. in rodents, it is primarily characterized by marked thymic atrophy and splenomegaly. cadmium induces apoptosis in mice and suppresses the immune functions. piperine, major alkaloid of piper longum linn. and piper nigrum linn. with a long history of medicinal value, has shown anti-apoptotic activity in vitro. thus, to delineate its role in vivo, piperine (2.5mg/kg/day, oral, 7 days) treated balb/c mice were ad ...200921783982
pharmacological basis for the medicinal use of black pepper and piperine in gastrointestinal disorders.dried fruits of piper nigrum (black pepper) are commonly used in gastrointestinal disorders. the aim of this study was to rationalize the medicinal use of pepper and its principal alkaloid, piperine, in constipation and diarrhea using in vitro and in vivo assays. when tested in isolated guinea pig ileum, the crude extract of pepper (pn.cr) (1–10 mg/ml) and piperine (3–300 μm) caused a concentration-dependent and atropine-sensitive stimulant effect. in rabbit jejunum, pn.cr (0.01–3.0 mg/ml) and p ...201020828313
involvement of serotonergic system in the antidepressant-like effect of piperine.piperine is a major alkaloid of black pepper (piper nigrum linn.) and long pepper (p. longum linn.), and its antidepressant-like effect has been previously demonstrated. the purpose of this study was to explore the possible contribution of the serotonergic system in the antidepressant-like effect of piperine in mice. the results showed that piperine significantly reduced the immobility time in the forced swim test and tail suspension test in mice. the anti-immobility effect of piperine in the fo ...201121477634
studies on the factors modulating indole-3-acetic acid production in endophytic bacterial isolates from piper nigrum and molecular analysis of ipdc gene.the study mainly aimed quantitative analysis of iaa produced by endophytic bacteria under various conditions including the presence of extract from piper nigrum. analysis of genetic basis of iaa production was also conducted by studying the presence and diversity of the ipdc gene among the selected isolates.201424916921
corrigendum: leishmanicidal activity of piper nigrum bioactive fractions is interceded via apoptosis in vitro and substantiated by th1 immunostimulatory potential in vivo.[this corrects the article on p. 1368 in vol. 6, pmid: 26696979.].201627379073
leishmanicidal activity of piper nigrum bioactive fractions is interceded via apoptosis in vitro and substantiated by th1 immunostimulatory potential in vivo.visceral leishmaniasis (vl) is a life-threatening protozoal infection chiefly impinging the rural and poor population in the tropical and sub-tropical countries. the deadly affliction is rapidly expanding after its association with aids, swiftly defying its status of a neglected disease. despite successful formulation of vaccine against canine leishmaniasis, no licensed vaccine is yet available for human vl, chemotherapy is in appalling state, and the development of new candidate drugs has been pa ...201526696979
inhibitory effects of black pepper (piper nigrum) extracts and compounds on human tumor cell proliferation, cyclooxygenase enzymes, lipid peroxidation and nuclear transcription factor-kappa-b.black pepper (piper nigrum) and hot pepper (capsicum spp.) are widely used in traditional medicines. although hot capsicum spp. extracts and its active principles, capsaicinoids, have been linked with anticancer and anti-inflammatory activities, whether black pepper and its active principle exhibit similar activities is not known. in this study, we have evaluated the antioxidant, anti-inflammatory and anticancer activities of extracts and compounds from black pepper by using proinflammatory tran ...201020839630
a randomised double-blind placebo-controlled study on the effects of a herbal toothpaste on gingival bleeding, oral hygiene and microbial variables.different systems of traditional medicine of the indian subcontinent, have used acacia chundra willd, adhatoda vasica nees., mimusops elengi l., piper nigrum l., pongamia pinnata l. pirerre, quercus infectoria olivier., syzygium aromaticum l., terminalia chebula retz., zingiber officinale roscoe., individually or in combinations, to cure oral diseases.201121542426
effect of piperine on the inhibition of nitric oxide (no) and tnf-alpha production.effect of piperine which is an alkaloid present in plants such as piper nigrum and piper longum on the production of nitric oxide (no) and tumor necrosis factor-alpha (tnf-alpha) level was analyzed using in vitro as well as in vivo systems. the level of nitrite in the lps stimulated balb/c mice (95.3 microm) was reduced in the piperine treated animals (25 microm) significantly. nitrite level in the concanavalin-a (con-a) treated control animals (83.1 microm) was also significantly reduced to 18. ...200319180797
β-caryophyllene oxide inhibits growth and induces apoptosis through the suppression of pi3k/akt/mtor/s6k1 pathways and ros-mediated mapks activation.both pi3k/akt/mtor/s6k1 and mitogen activated protein kinase (mapk) signaling cascades play an important role in cell proliferation, survival, angiogenesis, and metastasis of tumor cells. in the present report, we investigated the effects of β-caryophyllene oxide (cpo), a sesquiterpene isolated from essential oils of medicinal plants such as guava (psidium guajava), oregano (origanum vulgare l.), cinnamon (cinnamomum spp.) clove (eugenia caryophyllata), and black pepper (piper nigrum l.) on the ...201121924548
influence of piperine on ibuprofen induced antinociception and its pharmacokinetics.piperine (cas 94-62-2), an alkaloid obtained from piper nigrum and p. longum, is a known inhibitor of various enzymes (cyp isozymes) responsible for biotransformation of drugs. by inhibiting the metabolism of drugs, piperine improves the bioavailability of drugs. in the present study piperine (10 mg/kg) significantly increased the dose-dependent antinociceptive activity of ibuprofen evaluated by both acetic acid writhing and formalin test, when it was administered with ibuprofen. ibuprofen plasm ...201122029226
green tea polyphenol epigallocatechin-3-gallate shows therapeutic antioxidative effects in a murine model of colitis.leukocyte infiltration, up-regulation of proinflammatory cytokines and severe oxidative stress caused by increased amounts of reactive oxygen species are characteristics of inflammatory bowel disease. the catechin (2r,3r)-2-(3,4,5-trihydroxyphenyl)-3,4-dihydro-1(2h)-benzopyran-3,5,7-triol-3-(3,4,5-trihydroxybenzoate), named epigallocatechin-3-gallate, egcg, has been demonstrated to exert anti-inflammatory and antioxidative properties, reducing reactive oxygen species in the inflamed tissues. the ...201122325177
down-regulation of osmotin (pr5) gene by virus-induced gene silencing (vigs) leads to susceptibility of resistant piper colubrinum link. to the oomycete pathogen phytophthora capsici leonian.piper colubrinum link., a distant relative of piper nigrum l., is immune to the oomycete pathogen phytophthora capsici leonian that causes 'quick wilt' in cultivated black pepper (p. nigrum). the osmotin, pr5 gene homologue, earlier identified from p. colubrinum, showed significant overexpression in response to pathogen and defense signalling molecules. the present study focuses on the functional validation of p. colubrinum osmotin (pcosm) by virus induced gene silencing (vigs) using tobacco rat ...201526155671
in vivo effect of some home spices extracts on the toxoplasma gondii tachyzoites.toxoplasmosis drugs have the longest history and are still the first choice for most conditions. alternative drugs such as co-trimoxazole and tetracycline have been tried and acclaimed successful. the lack of general acceptance, however, is an indication that the results are not very convincing. a wide range of antibiotics is urgently needed for patients with drug reaction or resistance problems. the anti-toxoplasmic activity of water and ethanol extracts as well as the oil of some home spices ( ...200923012192
design, synthesis and trypanocidal evaluation of novel 1,2,4-triazoles-3-thiones derived from natural piperine.the work reported herein describes the synthesis and the assessment of the trypanocidal activity of thirteen new 1,2,4-triazole-3-thiones obtained from natural piperine, the main constituent of the dry fruits of piper nigrum. it is part of a research program aiming to use abundant and easily available natural products as starting materials for the design and synthesis of new molecules potentially useful as antiparasitic drugs. the variously substituted triazole derivatives were synthesized from ...201323760033
growth and survival of salmonella in ground black pepper (piper nigrum).a four serovar cocktail of salmonella was inoculated into ground black pepper (piper nigrum) at different water activity (aw) levels at a starting level of 4-5 log cfu/g and incubated at 25 and at 35 °c. at 35 °c and aw of 0.9886 ± 0.0006, the generation time in ground black pepper was 31 ± 3 min with a lag time of 4 ± 1 h. growth at 25 °c had a longer lag, but generation time was not statistically different from growth at 35 °c. the aw threshold for growth was determined to be 0.9793 ± 0.0027 a ...201223498196
analysis of the blackening of green pepper (piper nigrum linnaeus) berries.this paper investigates polyphenol oxidase (ppo) activity, reduced weight percentage after sun drying, and the changes in colour and appearance of green pepper (piper nigrum linnaeus) berries after blanching and sun drying. the results show that the degree of reduced weight percentage and browning in green pepper berries after blanching for 10 min is greater at 100°c than at 90 and 80°c. moreover, the samples blanched at 100°c for 10 min had the fastest water loss, but the lowest ppo activity. t ...201223411179
transfer rna derived small rnas targeting defense responsive genes are induced during phytophthora capsici infection in black pepper (piper nigrum l.).small rnas derived from transfer rnas were recently assigned as potential gene regulatory candidates for various stress responses in eukaryotes. in this study, we report on the cloning and identification of trna derived small rnas from black pepper plants in response to the infection of the quick wilt pathogen, phytophthora capsici. 5'trfs cloned from black pepper were validated as highly expressed during p. capsici infection. a high-throughput systematic analysis of the small rnaome (srnaome) r ...201627313593
in vitro antibacterial and antibiotic-potentiation activities of four edible plants against multidrug-resistant gram-negative species.the present study was designed to investigate the antibacterial activities of the methanol extracts of four cameroonian edible plants, locally used to treat microbial infections, and their synergistic effects with antibiotics against a panel of twenty nine gram-negative bacteria including multi-drug resistant (mdr) phenotypes expressing active efflux pumps.201323885762
properties for sourcing nigerian larvicidal plants.aedes aegypti is the primary vector of chikungunya, yellow and dengue fevers. dengue fever is the major cause of child morbidity and hospitalisation in some asian and african countries, while yellow fever is prevalent in nigeria. the development of resistance to the available insecticides has necessitated the continued search for safer ones from plants. eighteen plant extracts with ethnomedical claims of or demonstrated febrifuge, antimalarial, insecticidal and insect repellent biological activi ...201424950440
a biodegradable device for the controlled release of piper nigrum (piperaceae) standardized extract to control aedes aegypti (diptera, culicidae) larvae.the significant increase in dengue, zika, and chikungunya and the resistance of the aedes aegypti mosquito to major insecticides emphasize the importance of studying alternatives to control this vector. the aim of this study was to develop a controlled-release device containing piper nigrum extract and to study its larvicidal activity against aedes aegypti.201728001214
physiological and biochemical effects of botanical extract from piper nigrum linn (piperaceae) against the dengue vector aedes aegypti liston (diptera: culicidae).the leaves of piper nigrum l. (piperaceae) were evaluated for chemical constituents and mosquito larvicidal activity against the larvae of aedes aegypti. gc and gc-ms analyses revealed that the crude extracts contain 16 compounds. thymol (20.77%) and ç-elemene (10.42%) were identified as the major constituents followed by cyclohexene, 4-ethenyl-4-methyl-3-(1-methylethenyl)-1-(1 methylethyl)-, (3r-trans) (7.58%), 4,6-octadienoic acid, 2-acetyl-2-methyl-, ethyl ester (6.98), 2(3h)-furanone, 3,4-bi ...201526277727
aedes aegypti larvicide from the ethanolic extract of piper nigrum black peppercorns.due to unavailability of a vaccine and a specific cure to dengue, the focus nowadays is to develop an effective vector control method against the female aedes aegypti mosquito. this study aims to determine the larvicidal fractions from piper nigrum ethanolic extracts (pnpcme) and to elucidate the identity of the bioactive compounds that comprise these larvicidal fractions. larvicidal assay was performed by subjecting 3rd to 4th a. aegypti instar larvae to pnpcme of p. nigrum. the pnpcme exhibite ...201525118563
efficacy and safety of topical trikatu preparation in, relieving mosquito bite reactions: a randomized controlled trial.trikatu is composed of dried fruits of piper nigrum l and piper retrofractum vahl, and dried rhizomes of zingiber officinale r. although this preparation has been used to relieve pruritis, pain, and inflammation for a long time, there is no clinical evidence to confirm its efficacy and safety. therefore, we performed a double-blind, within person-randomized controlled study of 30 healthy volunteers to determine efficacy and safety of topical trikatu on mosquito bite reactions.201424559814
synergistic larvicidal effect and morphological alterations induced by ethanolic extracts of annona muricata and piper nigrum against the dengue fever vector aedes aegypti.phytopesticide combinations of different botanical sources are seldom reported. annona muricata seed and piper nigrum fruit ethanolic extracts enriched in acetogenins and piperamides, respectively, were synergistically used as larvicides against the dengue fever vector aedes aegypti.201323112098
could essential oils of green and black pepper be used as food preservatives?black and green pepper essential oils were used in this study in order to determine the chemical composition, in vitro antimicrobial activity against food spoilage microorganisms and in situ oils effect on food microorganism, after incorporation in chicken soup, by suggested methodology for calculation of growth inhibition concentrations (gic50). chemical analysis revealed a total of 34 components. the major constituent of black pepper oil was trans-caryophyllene (30.33 %), followed by limonene ...201526396402
transcriptome- assisted label-free quantitative proteomics analysis reveals novel insights into piper nigrum-phytophthora capsici phytopathosystem.black pepper (piper nigrum l.), a tropical spice crop of global acclaim, is susceptible to phytophthora capsici, an oomycete pathogen which causes the highly destructive foot rot disease. a systematic understanding of this phytopathosystem has not been possible owing to lack of genome or proteome information. in this study, we explain an integrated transcriptome-assisted label-free quantitative proteomics pipeline to study the basal immune components of black pepper when challenged with p. capsi ...201627379110
inhibition of quorum-sensing-mediated biofilm formation in cronobacter sakazakii strains.the present study investigated plant extracts for their anti-quorum-sensing (qs) potential to inhibit the biofilm formation in cronobacter sakazakii strains. the bioassay based on loss of pigment production by chromobacterium violaceum 026 and agrobacterium tumefaciens ntl4(pzlr4) was used for initial screening of the extracts. further, the effect of extracts on the inhibition of qs-mediated biofilm in c. sakazakii isolates was evaluated using standard crystal violet assay. the effect on biofilm ...201627460095
Insecticidal activity of isobutylamides derived from Piper nigrum against adult of two mosquito species, Culex pipiens pallens and Aedes aegypti.The insecticidal activity of Piper nigrum fruit-derived piperidine alkaloid (piperine) and N-isobutylamide alkaloids (pellitorine, guineensine, pipercide and retrofractamide A) against female adults of Culex pipiens pallens and Aedes aegypti was examined. On the basis of 24-h LD(50) values, the compound most toxic to female C. pipiens pallens was pellitorine (0.4?µg/?) followed by guineensine (1.9?µg/?), retrofractamide A (2.4?µg/?) and pipercide (3.2?µg/?). LD(50) value of chlorpyrifos was 0.03 ...201122010905
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