TitleAbstractYear(sorted ascending)
mechanism-based inhibition of cyp3a4 and cyp2d6 by indonesian medicinal plants.thirty samples of indonesian medicinal plants were tested for their mechanism-based inhibition on cytochrome p450 3a4 (cyp3a4) and cyp2d6 via erythromycin n-demethylation and dextromethorphan o-demethylation activities in human liver microsomes. from screening with 0 and 20min preincubation at 0.5mg/ml of methanol extracts, five plants (cinnamomum burmani bark, foeniculum vulgare seed, strychnos ligustrina wood, tinospora crispa stem, and zingiber cassumunar rhizome) showed more than 30% increas ...200616414224
isolation and amplification of genomic dna from recalcitrant dried berries of black pepper (piper nigrum l.)--a medicinal pepper is an important medicinal spice traded internationally. the extraction of high quality genomic dna for pcr amplification from dried black pepper is challenging because of the presence of the exceptionally large amount of oxidized polyphenolic compounds, polysaccharides and other secondary metabolites. here we report a modified hexadecyl trimethyl ammonium bromide (ctab) protocol by incorporating potassium acetate and a final peg precipitation step to isolate pcr amplifiable genomic ...200717914177
cytoprotective and immunomodulating properties of piperine on murine splenocytes: an in vitro study.piper longum linn. and piper nigrum linn. are conventionally used as immuno-enhancers in indian system of traditional medicine. the underlying mechanism remains unknown. the present study was therefore, undertaken to delineate the role of piperine (major alkaloid) in cadmium (cd) induced immuno-compromised murine splenocytes. the various biological determinants such as oxidative stress markers (reactive oxygen species and gsh), bcl-2 protein expression, mitochondrial membrane potential, caspase- ...200717706638
black pepper and its pungent principle-piperine: a review of diverse physiological pepper (piper nigrum) is one of the most widely used among spices. it is valued for its distinct biting quality attributed to the alkaloid, piperine. black pepper is used not only in human dietaries but also for a variety of other purposes such as medicinal, as a preservative, and in perfumery. many physiological effects of black pepper, its extracts, or its major active principle, piperine, have been reported in recent decades. dietary piperine, by favorably stimulating the digestive enzy ...200717987447
amides from piper nigrum l. with dissimilar effects on melanocyte proliferation in-vitro.melanocyte proliferation stimulants are of interest as potential treatments for the depigmentary skin disorder, vitiligo. piper nigrum l. (piperaceae) fruit (black pepper) water extract and its main alkaloid, piperine (1), promote melanocyte proliferation in-vitro. a crude chloroform extract of p. nigrum containing piperine was more stimulatory than an equivalent concentration of the pure compound, suggesting the presence of other active components. piperine (1), guineensine (2), pipericide (3), ...200717430636
potential use of piper nigrum ethanol extract against pyrethroid-resistant aedes aegypti larvae.fractionation of piper nigrum ethanol extract, biomonitored by assays on pyrethroid-resistant aedes aegypti larvae yielded isolation of the larvicidal amides piperolein-a and piperine. comparing lc50 values, the ethanol extract (0.98 ppm) was the most toxic, followed by piperolein-a (1.46 ppm) and piperine (1.53 ppm).200717876460
repellent activity of essential oils against cockroaches (dictyoptera: blattidae, blattellidae, and blaberidae) in commercial essential oils extracted from the plant species boesenbergia rotunda (l.) mansf., citrus hystrix dc., curcuma longa l., litsea cubeba (lour.) pers., piper nigrum l., psidium guajava l. and zingiber officinale roscoe, and naphthalene as a control, were evaluated for repellent activity against the three cockroach species periplaneta americana (l.), blattella germanica (l.) and neostylopyga rhombifolia (stoll) under laboratory conditions. the essential oil derived from citrus hystr ...200717883004
analysis of aromatic and terpenic constituents of pepper extracts by capillary original method based on cec has been developed for the determination of aromatic and terpenic compounds in extracts of spices obtained from piper nigrum. the method is based on the use of a fused silica capillary (effective length: 23.5 cm, internal diameter: 100 microm) packed with a c18 sorbent (packing length: 23 cm, particle size: 5 microm). the mobile phase is a 50 mm, ph 6.0 ammonium acetate/acn (10:90 v/v) mixture. applying a 30 kv voltage, the following 11 compounds were separated an ...200717444231
characterization and control of endophytic bacterial contaminants in in vitro cultures of piper spp., taxus baccata subsp. wallichiana, and withania somnifera.bacterial contamination is a serious problem that causes severe loss of in vitro grown cultures of a number of plants. this problem becomes even more acute if the bacterial contamination is of endophytic origin. in such cases, identification and characterization of the contaminants is essential for achieving specific control of the contaminants through selective use of antibiotic agents, especially if the routinely used contamination control methods practiced elsewhere in tissue culture studies ...200717496951
antimutagenic effects of piperine on cyclophosphamide-induced chromosome aberrations in rat bone marrow cells.piperine is a major pungent substance and active component of black pepper (piper nigrum linn.) and long pepper (piper longum linn.). both plants are used worldwide as household spices and condiments. they are also used as important ingredients in folklore medicine in many asian countries. therefore, it is of interest to study antimutagenic effects of piperine. in this study, its influence on chromosomes was investigated in rat bone marrow cells. male wistar rats were orally administered piperin ...200718260741
in vitro antioxidant activity of three piper species.reactive oxygen species are known to play a role in numerous pathological conditions, and antioxidants from plant sources have been of great help in modulating this effect. this study assesses the methanolic extracts of the leaves of three piper species (piper guineense schum and thonn., piper nigrum l. and piper umbellatum l.) (piperaceae) for their polyphenolic concentration and free radical scavenging activity. the folin-ciocalteu method was used to assess the polyphenolic concentration while ...200718285307
binding of bioactive phytochemical piperine with human serum albumin: a spectrofluorometric study.piperine, the bioactive alkaloid compound of the spice black pepper (piper nigrum) exhibits a wide range of beneficial physiological and pharmacological activities. being essentially water-insoluble, piperine is presumed to be assisted by serum albumin for its transport in blood. in this study, the binding of piperine to serum albumin was examined by employing steady state and time resolved fluorescence techniques. binding constant for the interaction of piperine with human serum albumin, which ...200717407131
acat inhibition of alkamides identified in the fruits of piper this study, via a bioactivity-guided fractionation of meoh extracts of the fruits of piper nigrum, alkamide (5) and five previously-identified alkamides were isolated. their structures were elucidated via spectroscopic analysis ((1)h, (13)c nmr and esi-ms), as follows: retrofractamide a (1), pipercide (2), piperchabamide d (3), pellitorin (4), dehydroretrofractamide c (5) and dehydropipernonaline (6). the ic(50) values determined for the compounds were 24.5 (1), 3.7 (2), 13.5 (3), 40.5 (4), 6 ...200717188313
piperine protects cisplatin-induced apoptosis via heme oxygenase-1 induction in auditory cells.piperine is a major component of black pepper, piper nigrum linn, used widely in traditional medicine. in this study, we examined whether piperine could protect house ear institute-organ of corti 1 (hei-oc1) cells against cisplatin-induced apoptosis through the induction of heme oxygenase (ho)-1 expression. piperine (10-100 microm) induced the expression of ho-1 in dose- and time-dependent manners. piperine also induced antioxidant response element-luciferase and translocated nuclear factor-e2-r ...200717418561
testosterone 5alpha-reductase inhibitory active constituents of piper nigrum leaf.previously we reported that piper nigrum leaf extract showed a potent stimulation effect on melanogenesis and that (-)-cubebin (1) and (-)-3,4-dimethoxy-3,4-desmethylenedioxycubebin (2) were isolated as active constituents. as a part of our continuous studies on piper species for the development of cosmetic hair-care agents, testosterone 5alpha-reductase inhibitory activity of aqueous ethanolic extracts obtained from several different parts of six piper species, namely piper nigrum, p. methystic ...200718057734
in vitro antioxidant activity of pet ether extract of black investigate the in vitro antioxidant activity of different fractions (r1, r2 and r3) obtained from pet ether extract of black pepper fruits (piper nigrum linn.)200820040947
chemical compositions and antioxidant properties of essential oils from nine species of turkish plants obtained by supercritical carbon dioxide extraction and steam distillation.chemical compositions and antioxidant activities of essential oils from nine different species of turkish plants, namely melissa officinalis l., rosmarinus officinalis l., cuminum cyminum l., piper nigrum l., lavandula stoechas spp., foeniculum vulgare, pimpinella anisum l., thymus serpyllum and liquidamber orientalis mill., were studied. essential oils were obtained by supercritical carbon dioxide (scco2) extraction and steam distillation, and were analyzed by gas chromatography-mass spectromet ...200819382349
antispasmodic effect of piper nigrum fruit hot water extract on rat ileum.the aim of this study was to investigate the effect of black pepper fruit hot water extract (bpe) on rat ileum contractility and the mechanism(s) of its action. the extract was prepared by adding black pepper powder to boiling distilled water followed by evaporated the solvent. ileum was dissected from male adult rat (wistar) and in tyrode solution the tissue contractions were recorded by an isotonic transducer under 1 g tension. the cumulative concentrations of the bpe (0.0625-1 mg ml(-1)) redu ...200818817253
physicochemical and microbiological qualities of steamed and irradiated ground black pepper (piper nigrum l.).the effects of steam and irradiation treatments on the physicochemical properties (moisture content, ph, extractable yield, reducing sugar, soluble pigment, antioxidant activity, piperine, hunter's color, and sensory attributes) and microbiological quality (total aerobic bacteria, coliforms, and yeasts and molds) of ground black pepper stored at refrigerated and room temperatures for 6 months were compared and evaluated. irradiation resulted in a higher microbial reduction in pepper, with minima ...200818522396
[control in vitro tick (boophilus microplus; acari: ixodidae) through plant extracts].control in vitro tick (boophilus microplus; acari: ixodidae) through plant extracts. hydro-alcohol extracts of ten tropical plants were tested by the immersion method on mature ticks (boophilus microplus canestrini 1887). the biological evaluation was made with raw hydro alcoholic extracts (r-oh) to determine the most promissory compounds, which later were fractioned in their polar and non-polar parts. the polar parts showed a significantly inferior effect. the species that produced the highest ...200818624244
studies on effect of piperine on oral bioavailability of ampicillin and norfloxacin.ampicillin and norfloxacin are used to treat variety of bacterial infections. these two drugs have low oral bioavailability. co-administration of piperine (20 mg/kg), an alkaloid from piper nigrum l. enhanced oral bioavailability of ampicillin and norfloxacin in animal model. this is reflected in various pharmacokinetic measurements like cmax, tmax, auc and t(1/2) of the above antibiotics in animal model.200820161946
molecular target of piperine in the inhibition of lipid droplet accumulation in alkaloid piperine isolated from the piper nigrum was found to inhibit lipid droplet accumulation in mouse macrophages, and especially inhibited cholesteryl ester (ce) synthesis (ic50: 25 microm). the metabolism of cholesterol from lysosome to lipid droplet was inhibited with a similar ic50 (18 microm), indicating that the site of inhibition is one of the steps between the lysosomes and the endoplasmic reticulum. therefore, effects of piperine on acyl-coa:cholesterol acyltransferase (acat) act ...200818520030
piperine, the potential functional food for mood and cognitive disorders.the effect of piperine, the main alkaloid from piper nigrum, on the central nervous system is not clearly known until now. in the present study, male wistar rats were administered piperine at various doses ranging from 5, 10 and 20mg/kg bw once daily for 4 weeks and the animals were determined the neuropharmacological activity after single, 1, 2, 3 and 4 weeks of treatment. the results showed that piperine at all dosage range used in this study possessed anti-depression like activity and cogniti ...200818639606
alkamides from the fruits of piper longum and piper nigrum displaying potent cell adhesion inhibition.eight alkamides 1-8 were isolated by bioassay-guided isolation of etoh extracts of the fruits of piper longum and piper nigum (piperaceae). their structures were elucidated by spectroscopic analysis ((1)h, (13)c nmr, and esi-ms) as follows: guineensine (1), retrofracamide c (2), (2e,4z,8e)-n-[9-(3,4-methylenedioxyphenyl)-2,4,8-nonatrienoyl]piperidine (3), pipernonaline (4), piperrolein b (5), piperchabamide d (6), pellitorin (7), and dehydropipernonaline (8). their compounds 3-5, 7, and 8 inhibi ...200818672369
histamine release inhibitory activity of piper nigrum leaf.oral administration of a methanolic extract of piper nigrum leaf (pn-ext, 50, 200 and 500 mg/kg) showed a potent dose-dependent inhibition of dinitrofluorobenzene (dnfb)-induced cutaneous reaction at 1 h [immediate phase response (ipr)] after and 24 h [late phase response (lpr)] after dnfb challenge in mice which were passively sensitized with anti-dinitrophenyl (dnp) ige antibody. ear swelling inhibitory effect of pn-ext (50, 200 and 500 mg/kg, per os (p.o.)) on very late phase response (vlpr) ...200818827366
fumigant toxicity of essential oils from some common spices against pulse beetle, callosobruchus chinensis (coleoptera: bruchidae).in the present study, the essential oil from seven common spices, anethum graveolens, cuminum cyminum, illicium verum, myristica fragrans, nigella sativa, piper nigrum and trachyspermum ammi was isolated and its insecticidal, oviposition, egg hatching and developmental inhibitory activities were determined against pulse beetle, callosobruchus chinensis. essential oils were isolated by hydrodistillation method using clevenger apparatus. these essential oils caused death of adults and larvae of ca ...200818270466
a rapid method for isolation of piperine from the fruits of piper nigrum linn.a simple, rapid and efficient method has been developed for the isolation of piperine from the fruits of piper nigrum. the method involves extraction of the fruit powder with glacial acetic acid, from which piperine is partitioned into chloroform and subsequently crystallized. the identity of the compound was confirmed by its melting point, comparison of uv, ir, and mass spectral data with those from a reference standard, and co-chromatography with the reference standard using thin-layer chromat ...200818404304
a new natural product and insecticidal amides from seeds of piper nigrum linn.studies on the petroleum ether soluble and insoluble fraction of ethanol extract of dried ground seeds of piper nigrum resulted in the isolation and structure elucidation of 1 new and 11 known compounds which include 3 hitherto unreported constituents, namely, cinnamylideneacetone, 3,4-methylenedioxyphenylpropiophenone and 2-hydroxy-4,5-methylenedioxypropiophenone from this plant.200818855209
alkaloids from piper sarmentosum and piper nigrum.detailed chemical studies on the roots of piper sarmentosum and piper nigrum have resulted in several alkaloids. the roots of p. sarmentosum gave a new aromatic compound, 1-nitrosoimino-2,4,5-trimethoxybenzene (1). piper nigrum roots gave pellitorine (2), (e)-1-[3',4'-(methylenedioxy)cinnamoyl]piperidine (3), 2,4-tetradecadienoic acid isobutyl amide (4), piperine (5), sylvamide (6), cepharadione a (7), piperolactam d (8) and paprazine (9). structural elucidation of these compounds was achieved t ...200919809914
stability-indicating reversed-phase liquid chromatographic methods for the determination of aconitine and piperine in a polyherbal formulation.simple and rapid stability-indicating hplc methods were developed for the individual analysis of aconitine (acn) and piperine (pin) in mahamrutynjaya rasa, an herbal dosage form containing aconitum ferox, piper nigrum, and piper longum in combination. separation of the acn from its major and minor degradation products was successfully achieved on a reversed-phase c18 column (250 x 4.6 mm id, 5 microm particle size), with isocratic elution using a mixture of acetonitrile-kh2po4 buffer (10 mm, ph ...200919714970
endophytic bacterial flora in root and stem tissues of black pepper (piper nigrum l.) genotype: isolation, identification and evaluation against phytophthora isolate and identify black pepper (piper nigrum l) associated endophytic bacteria antagonistic to phytophthora capsici causing foot rot disease.200919018963
larvicidal effects of crude extracts of dried ripened fruits of piper nigrum against culex quinquefasciatus larval instars. 200919502696
[antioxidant properties of essential oils].by the method of capillary gas-liquid chromatography we studied antioxidant properties and stability during the storage of hexane solutions of 14 individual essential oils from black and white pepper (piper nigrum l.), cardamom (elettaria cardamomum l.), nutmeg (myristica fragrans houtt.), mace (myristica fragrans houtt), juniperberry (juniperus communis l.), seed of fennel (foeniculum vulgare mill., var. dulce thelling), caraway (carvum carvi l.), dry leaves of cinnamon (cinnamomum zeylanicum b ...200920067158
identifying sequences potentially related to resistance response of piper tuberculatum to fusarium solani f. sp. piperis by suppression subtractive hybridization.piper tuberculatum is an exotic piper from the amazon region that shows resistance to infection by fusarium solani f. sp. piperis, causal agent of fusarium disease in black pepper (piper nigrum l.). in this work we aimed to study the interaction between p. tuberculatum and f. solani f. sp. piperis at a molecular level, using suppression subtractive hybridization to identify genes potentially related to fusarium disease resistance. comparative sequence analysis confirmed that clones isolated here ...200920001904
immunomodulatory role of piperine in cadmium induced thymic atrophy and splenomegaly in mice.cadmium being a potent immunotoxicant, affects both humoral and cell mediated immunity. in rodents, it is primarily characterized by marked thymic atrophy and splenomegaly. cadmium induces apoptosis in mice and suppresses the immune functions. piperine, major alkaloid of piper longum linn. and piper nigrum linn. with a long history of medicinal value, has shown anti-apoptotic activity in vitro. thus, to delineate its role in vivo, piperine (2.5mg/kg/day, oral, 7 days) treated balb/c mice were ad ...200921783982
in vivo effect of some home spices extracts on the toxoplasma gondii tachyzoites.toxoplasmosis drugs have the longest history and are still the first choice for most conditions. alternative drugs such as co-trimoxazole and tetracycline have been tried and acclaimed successful. the lack of general acceptance, however, is an indication that the results are not very convincing. a wide range of antibiotics is urgently needed for patients with drug reaction or resistance problems. the anti-toxoplasmic activity of water and ethanol extracts as well as the oil of some home spices ( ...200923012192
differential induction of chitinase in piper colubrinum in response to inoculation with phytophthora capsici, the cause of foot rot in black pepper.plant chitinases have been of particular interest since they are known to be induced upon pathogen invasion. inoculation of piper colubrinum leaves with the foot rot fungus, phytophthora capsici leads to increase in chitinase activity. a marked increase in chitinase activity in the inoculated leaves was observed, with the maximum activity after 60 h of inoculation and gradually decreased thereafter. older leaves showed more chitinase activity than young leaves. the level of chitinase in black pe ...200923961037
antispermatogenic and antifertility effects of fruits of piper nigrum l. in mice.effect of oral administration (25 and 100 mg/kg body wt/day, for 20 and 90 days) of fruit powder of piper nigrum l. on the male reproductive organs of mice, parkes strain, was investigated. various reproductive end points such as organs weight, histopathology, sperm parameters, sialic acid and fructose contents, and fertility indices were assessed. histologically, testes in treated mice, except in those treated with 100 mg of dose for 90 days, showed non-uniform degenerative changes in the semin ...200919957882
chemistry and in vitro antioxidant activity of volatile oil and oleoresins of black pepper (piper nigrum).essential oil and oleoresins (ethanol and ethyl acetate) of piper nigrum were extracted by using clevenger and soxhlet apparatus, respectively. gc-ms analysis of pepper essential oil showed the presence of 54 components representing about 96.6% of the total weight. beta-caryophylline (29.9%) was found as the major component along with limonene (13.2%), beta-pinene (7.9%), sabinene (5.9%), and several other minor components. the major component of both ethanol and ethyl acetate oleoresins was fou ...200919456163
pharmacological basis for the medicinal use of black pepper and piperine in gastrointestinal disorders.dried fruits of piper nigrum (black pepper) are commonly used in gastrointestinal disorders. the aim of this study was to rationalize the medicinal use of pepper and its principal alkaloid, piperine, in constipation and diarrhea using in vitro and in vivo assays. when tested in isolated guinea pig ileum, the crude extract of pepper ( (1–10 mg/ml) and piperine (3–300 μm) caused a concentration-dependent and atropine-sensitive stimulant effect. in rabbit jejunum, (0.01–3.0 mg/ml) and p ...201020828313
piperine, the main alkaloid of thai black pepper, protects against neurodegeneration and cognitive impairment in animal model of cognitive deficit like condition of alzheimer's disease.recently, numerous medicinal plants possessing profound central nervous system effects and antioxidant activity have received much attention as food supplement to improve cognitive function against cognitive deficit condition including in alzheimer's disease condition. based on this information, the effect of piperine, a main active alkaloid in fruit of piper nigrum, on memory performance and neurodegeneration in animal model of alzheimer's disease have been investigated. adult male wistar rats ...201020034530
inhibition of lipopolysaccharide-induced inflammatory responses by piperine.piperine, a main component of piper longum linn. and piper nigrum linn., is a plant alkaloid with a long history of medical use. piperine exhibits anti-inflammatory activity; however, the underlying mechanism remains unknown. we examined the effects of piperine on lipopolysaccharide (lps)-induced inflammatory responses. administration of piperine inhibited lps-induced endotoxin shock, leukocyte accumulation and the production of tumor necrosis factor-alpha (tnf-alpha), but not of interleukin (il ...201020621590
adsorption and removal kinetics of phosphonate from water using natural adsorbents.the removal of phosphonate from water was studied using some natural adsorbents. potassium phosphonate is a fungicide used for the control of phytophthora capsici, which is prevalent in black pepper (piper nigrum l.). batch adsorption kinetic experiments were conducted on the adsorption of phosphonate onto the adsorbents. the concentration of phosphonate was measured on a high-performance liquid chromatograph fitted with a conductivity detector. the percentage removal of phosphonate by powdered ...201020112539
hplc-based activity profiling: discovery of piperine as a positive gaba(a) receptor modulator targeting a benzodiazepine-independent binding site.a plant extract library was screened for gaba(a) receptor activity making use of a two-microelectrode voltage clamp assay on xenopus laevis oocytes. an ethyl acetate extract of black pepper fruits [piper nigrum l. (piperaceae) 100 microg/ml] potentiated gaba-induced chloride currents through gaba(a) receptors (composed of alpha(1), beta(2), and gamma(2s) subunits) by 169.1 +/- 2.4%. with the aid of an hplc-based activity profiling approach, piperine (5) was identified as the main active compound ...201020085307
inhibitory effects of black pepper (piper nigrum) extracts and compounds on human tumor cell proliferation, cyclooxygenase enzymes, lipid peroxidation and nuclear transcription pepper (piper nigrum) and hot pepper (capsicum spp.) are widely used in traditional medicines. although hot capsicum spp. extracts and its active principles, capsaicinoids, have been linked with anticancer and anti-inflammatory activities, whether black pepper and its active principle exhibit similar activities is not known. in this study, we have evaluated the antioxidant, anti-inflammatory and anticancer activities of extracts and compounds from black pepper by using proinflammatory tran ...201020839630
characterisation of phytophthora capsici isolates from black pepper in vietnam.phytophthora foot rot of black pepper caused by phytophthora capsici is a major disease of black pepper (piper nigrum) throughout vietnam. to understand the population structure of p. capsici, a large collection of p. capsici isolates from black pepper was studied on the basis of mating type, random amplified microsatellites (rams) and repetitive extragenic palindromic (rep) fingerprinting. two mating types a1 and a2 were detected in four provinces in two climatic regions, with a1:a2 ratios rang ...201020960972
antioxidant and anti-atherogenic activities of three piper species on atherogenic diet fed hamsters.atherogenic diet is known to induce high plasma lipid concentration, oxidative stress and early atherosclerosis. antioxidants have potentials to counter the effect of atherogenic diet. the present research aims at evaluating the antioxidant and anti-atherosclerotic activities of three piper species (piper guineense, piper nigrum and piper umbellatum) on atherogenic diet fed hamsters. hamsters divided into 8 groups: normal control, atherosclerotic control and six test groups. the normal animals f ...201021035316
the plant alkaloid piperine as a potential inhibitor of ethidium bromide efflux in mycobacterium smegmatis.piperine, a major plant alkaloid found in black pepper (piper nigrum) and long pepper (piper longum), has shown potential for inhibiting the efflux pump (ep) of staphylococcus aureus. in this study, a modulation assay showed that piperine could decrease the mic of ethidium bromide (etbr) twofold at 32 μg ml(-1) and fourfold at 64 μg ml(-1) against mycobacterium smegmatis mc(2) 155 atcc 700084. a real-time, 96-well plate fluorometric method was employed to evaluate the ep inhibition ...201021051548
inhibition of chemiluminescence and chemotactic activity of phagocytes in vitro by the extracts of selected medicinal plants.the methanol extracts of 20 selected medicinal plants were investigated for their effects on the respiratory burst of human whole blood, isolated human polymorphonuclear leukocytes (pmns) and isolated mice macrophages using a luminol/lucigenin-based chemiluminescence assay. we also tested the effect of the extracts on chemotactic migration of pmns using the boyden chamber technique. the extracts of curcuma domestica l., phyllanthus amarus schum & thonn and c. xanthorrhiza roxb. were the samples ...201021184195
in vitro investigation of the potential immunomodulatory and anti-cancer activities of black pepper (piper nigrum) and cardamom (elettaria cardamomum).although the immunomodulatory effects of many herbs have been extensively studied, research related to possible immunomodulatory effects of various spices is relatively scarce. here, the potential immunomodulatory effects of black pepper and cardamom are investigated. our data show that black pepper and cardamom aqueous extracts significantly enhance splenocyte proliferation in a dose-dependent, synergistic fashion. enzyme-linked immunosorbent assay experiments reveal that black pepper and carda ...201020210607
pellitorine, a potential anti-cancer lead compound against hl6 and mct-7 cell lines and microbial transformation of piperine from piper nigrum.pellitorine (1), which was isolated from the roots of piper nigrum, showed strong cytotoxic activities against hl60 and mct-7 cell lines. microbial transformation of piperine (2) gave a new compound 5-[3,4-(methylenedioxy)phenyl]-pent-2-ene piperidine (3). two other alkaloids were also found from piper nigrum. they are (e)-1-[3',4'-(methylenedioxy)cinnamoyl]piperidine (4) and 2,4-tetradecadienoic acid isobutyl amide (5). these compounds were isolated using chromatographic methods and their struc ...201020428051
tissue distribution & elimination of capsaicin, piperine & curcumin following oral intake in rats.curcumin, capsaicin and piperine--the bioactive compounds present in spices--turmeric (curcuma longa), red pepper (capsicum annuum) and black pepper (piper nigrum) respectively, have a considerable portion of structural homology. tissue distribution and elimination of these three structurally similar bioactive compounds was examined following their oral intake in rats.201020516541
larvicidal potential of ethanolic extracts of dried fruits of three species of peppercorns against different instars of an indian strain of dengue fever mosquito, aedes aegypti l. (diptera: culicidae).larvicidal bioassay was carried out in the laboratory to assess the potential of ethanolic extracts of dried fruits of three species of peppercorns: long pepper, piper longum l., black pepper, piper nigrum, and white pepper, piper nigrum against the different instars of field-collected indian strain of dengue fever mosquito (aedes aegypti l.). the investigations established the larvicidal potential of all the varieties of pepper fruits against ae. aegypti. against early fourth instar, the ethano ...201020549234
piperine, a piperidine alkaloid from piper nigrum re-sensitizes p-gp, mrp1 and bcrp dependent multidrug resistant cancer cells.over-expression of p-gp, mrp1 and bcrp in tumor cells is one of the important mechanisms leading to multidrug resistance (mdr), which impairs the efficacy of chemotherapy. p-gp, mrp1 and bcrp are abc (atp-binding cassette) transporters, which can expel a variety of lipophilic anti-cancer drugs and protect tumor cells. during a screening of mdr reversal agents among alkaloids of various structural types, a piperidine alkaloid, piperine (a main piperidine alkaloid in piper nigurm) was identified a ...201121802927
documentation and quantitative analysis of the local knowledge on medicinal plants among traditional siddha healers in virudhunagar district of tamil nadu, india.india has a population with high degree of medical pluralism. siddha system of indian traditional medicine is practiced dominantly by the people in tamil nadu. the traditionally trained siddha healers still play an important role in the rural health care. their knowledge is comparatively more vulnerable than the documented traditional knowledge. thus, the present study was aimed to document and quantitatively analyze the local knowledge of the traditional siddha healers in virudhunagar district ...201121718779
potent α-amylase inhibitory activity of indian ayurvedic medicinal plants.indian medicinal plants used in the ayurvedic traditional system to treat diabetes are a valuable source of novel anti-diabetic agents. pancreatic α-amylase inhibitors offer an effective strategy to lower the levels of post-prandial hyperglycemia via control of starch breakdown. in this study, seventeen indian medicinal plants with known hypoglycemic properties were subjected to sequential solvent extraction and tested for α-amylase inhibition, in order to assess and evaluate their inhibitory po ...201121251279
larvicidal and repellent activity of medicinal plant extracts from eastern ghats of south india against malaria and filariasis evaluate the larvicidal and repellent activities of ethyl acetate and methanol extracts of acacia concinna (a. concinna), cassia siamea (c. siamea), coriandrum sativum (c. sativum),cuminum cyminum (c. cyminum), lantana camara (l. camara), nelumbo nucifera (n. nucifera) phyllanthus amarus (p. amarus), piper nigrum (p. nigrum) and trachyspermum ammi (t. ammi) against anopheles stephensi (an. stephensi) and culex quinquefasciatus (cx. quinquefasciatus).201121967692
Studies on antimicrobial activities of solvent extracts of different spices.The antimicrobial activities of the ethyl acetate, acetone and methanol extract of 12 plant species were studied. The extract of Capsicum annuum (red pepper) (fruit) Zingiber officinale (ginger) (root), Cuminum cyminum (cumin), Alpinia ficinarum (galingale), Coriandrum sativum (coriander), Cinnamomun zeylanicum Nees (cinnamomun), Origanum onites L. (thyme), Folium sennae (senna), Eugenia caryophyllata (cloves), Flos tiliae (lime), Folium menthae crispae (peppermint) and Piper nigrum (blackpepper ...201121882663
acetylcholinesterase inhibitory activity of thai traditional nootropic remedy and its herbal ingredients.the incidence of alzheimer disease (ad) is increasing every year in accordance with the increasing of elderly population and could pose significant health problems in the future. the use of medicinal plants as an alternative prevention or even for a possible treatment of the ad is, therefore, becoming an interesting research issue. acetylcholinesterase (ache) inhibitors are well-known drugs commonly used in the treatment of ad. the aim of the present study was to screen for ache inhibitory activ ...201122619927
medico - botanical study of yercaud hills in the eastern ghats of tamil nadu, india.the study reports medicinal plant survey was conceded in yercaud hills ranges of eastern ghats, tamil nadu, india. the study primarily based on field surveys conducted throughout the hills, where dwellers provided information on plant species used as medicine, plant parts used to prepare the remedies and ailments to which the remedies were prescribed. the study resulted about 48- plant species belonging to 45- genera and 29- families of medicinal plants related to folk medicine used by the local ...201122557438
green tea polyphenol epigallocatechin-3-gallate shows therapeutic antioxidative effects in a murine model of colitis.leukocyte infiltration, up-regulation of proinflammatory cytokines and severe oxidative stress caused by increased amounts of reactive oxygen species are characteristics of inflammatory bowel disease. the catechin (2r,3r)-2-(3,4,5-trihydroxyphenyl)-3,4-dihydro-1(2h)-benzopyran-3,5,7-triol-3-(3,4,5-trihydroxybenzoate), named epigallocatechin-3-gallate, egcg, has been demonstrated to exert anti-inflammatory and antioxidative properties, reducing reactive oxygen species in the inflamed tissues. the ...201122325177
antibacterial activity of natural spices on multiple drug resistant escherichia coli isolated from drinking water, bangladesh.spices traditionally have been used as coloring agents, flavoring agents, preservatives, food additives and medicine in bangladesh. the present work aimed to find out the antimicrobial activity of natural spices on multi-drug resistant escherichia coli isolates.201121406097
studies on the effects of phosphine on salmonella enterica serotype enteritidis in culture medium and in black pepper (piper nigrum).the effect of phosphine on salmonella enterica serotype enteritidis inoculated in culture medium and in black pepper grains (piper nigrum), as well as on the reduction of the microbial load of the dried and moisturized product, was verified. the postfumigation effect was verified in inoculated samples with 0.92 and 0.97 water activity (a(w)) exposed to 6 g/m(3) phosphine for 72 h, dried to 0.67 a(w), and stored for 24, 48, and 72 h. no decreases were observed in salmonella enteritidis population ...201121477485
β-caryophyllene oxide inhibits growth and induces apoptosis through the suppression of pi3k/akt/mtor/s6k1 pathways and ros-mediated mapks activation.both pi3k/akt/mtor/s6k1 and mitogen activated protein kinase (mapk) signaling cascades play an important role in cell proliferation, survival, angiogenesis, and metastasis of tumor cells. in the present report, we investigated the effects of β-caryophyllene oxide (cpo), a sesquiterpene isolated from essential oils of medicinal plants such as guava (psidium guajava), oregano (origanum vulgare l.), cinnamon (cinnamomum spp.) clove (eugenia caryophyllata), and black pepper (piper nigrum l.) on the ...201121924548
simultaneous determination of bioactive compounds in piper nigrum l. and a species comparison study using hplc-pda.piper nigrum l. is a traditional medicine widely used in india for illnesses such as constipation, diarrhoea, earache, gangrene, heart disease, hernia, hoarseness, indigestion, insect bites, insomnia, joint pain, liver problems, lung disease, oral abscesses, sunburn, tooth decay and toothaches. in this study, six bioactive compounds, namely piperine (1), pellitorine (2), guineensine (3), pipnoohine (4), trichostachine (5) and piperonal (6) were quantified in different extracts of p. nigrum l. an ...201121854175
Insecticidal activity of isobutylamides derived from Piper nigrum against adult of two mosquito species, Culex pipiens pallens and Aedes aegypti.The insecticidal activity of Piper nigrum fruit-derived piperidine alkaloid (piperine) and N-isobutylamide alkaloids (pellitorine, guineensine, pipercide and retrofractamide A) against female adults of Culex pipiens pallens and Aedes aegypti was examined. On the basis of 24-h LD(50) values, the compound most toxic to female C. pipiens pallens was pellitorine (0.4?µg/?) followed by guineensine (1.9?µg/?), retrofractamide A (2.4?µg/?) and pipercide (3.2?µg/?). LD(50) value of chlorpyrifos was 0.03 ...201122010905
mediation of cholino-piperine like receptors by extracts of piper nigrum induces melanin dispersion in rana tigerina tadpole melanophores.the present study was carried out to determine the effects of lyophilized dried fruit extracts of piper nigrum and pure piperine on the tadpole melanophores of frog rana tigerina which offer excellent in vitro opportunities for studying the effects of pharmacological and pharmaceutical agents. the nature of specific cellular receptors present on the neuro-melanophore junction and their involvement in pigmentary responses has been explored.201121663558
piperine ameliorates the severity of cerulein-induced acute pancreatitis by inhibiting the activation of mitogen activated protein kinases.piperine is a phenolic component of black pepper (piper nigrum) and long pepper (piper longum), fruits used in traditional asian medicine. our previous study showed that piperine inhibits lipopolysaccharide-induced inflammatory responses. in this study, we investigated whether piperine reduces the severity of cerulein-induced acute pancreatitis (ap). administration of piperine reduced histologic damage and myeloperoxidase (mpo) activity in the pancreas and ameliorated many of the examined labora ...201121663734
suppression of phorbol-12-myristate-13-acetate-induced tumor cell invasion by piperine via the inhibition of pkca/erk1/2-dependent matrix metalloproteinase-9 expression.piperine is a major component of black pepper, piper nigrum linn, used widely in traditional medicine. several previous studies reported that piperine possesses various beneficial biological activities including antioxidant, anti-tumor and anti-inflammation properties. in the present study, we investigated the inhibitory effects of piperine on tumor invasion and migration and the possible mechanisms involved using human fibrosarcoma ht-1080 cells. we found that piperine suppresses pma-enhanced m ...201121354279
a randomised double-blind placebo-controlled study on the effects of a herbal toothpaste on gingival bleeding, oral hygiene and microbial variables.different systems of traditional medicine of the indian subcontinent, have used acacia chundra willd, adhatoda vasica nees., mimusops elengi l., piper nigrum l., pongamia pinnata l. pirerre, quercus infectoria olivier., syzygium aromaticum l., terminalia chebula retz., zingiber officinale roscoe., individually or in combinations, to cure oral diseases.201121542426
involvement of serotonergic system in the antidepressant-like effect of piperine.piperine is a major alkaloid of black pepper (piper nigrum linn.) and long pepper (p. longum linn.), and its antidepressant-like effect has been previously demonstrated. the purpose of this study was to explore the possible contribution of the serotonergic system in the antidepressant-like effect of piperine in mice. the results showed that piperine significantly reduced the immobility time in the forced swim test and tail suspension test in mice. the anti-immobility effect of piperine in the fo ...201121477634
Selective molecular sequestration with concurrent natural product functionalization and derivatization: from crude natural product extracts to a single natural product derivative in one step.A resin-bound nitroso compound sequestered a single unexpected component from crude plant seed extracts. Several plants, including Piper nigrum, Eugenia caryophyllata, and Pimenta dioica, were extracted with organic solvent in the presence of a nitroso-containing resin. The nitroso resin selectively sequestered a single compound, ß-caryophyllene, via a chemo- and regioselective ene reaction. The ene product was released from the resin, and proper selection of the solid-phase linker and cleavage ...201122059469
Safety assessment of Zanthoxylum alatum Roxb. essential oil, its antifungal, antiaflatoxin, antioxidant activity and efficacy as antimicrobial in preservation of Piper nigrum L. fruits.The investigation deals with antifungal, antiaflatoxin and antioxidant efficacy of Zanthoxylum alatum Roxb. essential oil (EO), its two major constituents and their comparison with five commonly used organic acid preservatives. The chemical profile of EO, characterized through GC and GC-MS analysis, revealed linalool (56.10%) and methyl cinnamate (19.73%) as major components. The EO, linalool and methyl cinnamate completely inhibited the growth of a toxigenic strain of A. flavus (LHP-10) as well ...201122137251
influence of piperine on ibuprofen induced antinociception and its pharmacokinetics.piperine (cas 94-62-2), an alkaloid obtained from piper nigrum and p. longum, is a known inhibitor of various enzymes (cyp isozymes) responsible for biotransformation of drugs. by inhibiting the metabolism of drugs, piperine improves the bioavailability of drugs. in the present study piperine (10 mg/kg) significantly increased the dose-dependent antinociceptive activity of ibuprofen evaluated by both acetic acid writhing and formalin test, when it was administered with ibuprofen. ibuprofen plasm ...201122029226
flash chromatography on cartridges for the separation of plant extracts: rules for the selection of chromatographic conditions and comparison with medium pressure liquid chromatography.empirical rules for the selection of chromatographic conditions on flash chromatography cartridges were developed, with an emphasis on gradient mode. these rules were then tested with separation of extracts from important medicinal plants including curcuma xanthorrhiza, piper nigrum and salvia milthiorrhiza. sepacore® cartridges enabled a good separation of compounds with a broad range of polarity, as typically found in plant extracts. the chromatographic resolution remained, however, lower than ...201120804823
potential mechanisms of hypocholesterolaemic effect of thai spices/dietary extracts.several thai spices/dietary ingredients were previously shown to have hypocholesterolaemic effects. these studies were mostly conducted in animal models in which the mechanisms of action were not yet well-established. therefore, this study aimed to investigate the potential mechanism of hypocholesterolaemic action of 12 selected plants, namely hibiscus sabdariffa l., moringa oleifera lam., cucurbita moschata duchesne, ananas comosus (l.) merr., zingiber officinale, morus alba l., camellia sinens ...201120623425
antimalarial activities of medicinal plants traditionally used in the villages of dharmapuri regions of south ethnopharmacological investigation of medicinal plants traditionally used to treat diseases associated with fevers in dharmapuri region of south india was undertaken. twenty four plants were identified and evaluated for their in vitro activity against plasmodium falciparum and assessed for cytotoxicity against hela cell line.201222433533
growth and survival of salmonella in ground black pepper (piper nigrum).a four serovar cocktail of salmonella was inoculated into ground black pepper (piper nigrum) at different water activity (aw) levels at a starting level of 4-5 log cfu/g and incubated at 25 and at 35 °c. at 35 °c and aw of 0.9886 ± 0.0006, the generation time in ground black pepper was 31 ± 3 min with a lag time of 4 ± 1 h. growth at 25 °c had a longer lag, but generation time was not statistically different from growth at 35 °c. the aw threshold for growth was determined to be 0.9793 ± 0.0027 a ...201223498196
high-throughput sequencing of black pepper root pepper (piper nigrum l.) is one of the most popular spices in the world. it is used in cooking and the preservation of food and even has medicinal properties. losses in production from disease are a major limitation in the culture of this crop. the major diseases are root rot and foot rot, which are results of root infection by fusarium solani and phytophtora capsici, respectively. understanding the molecular interaction between the pathogens and the host's root region is important for obt ...201222984782
inhibition of monoamine oxidase by derivatives of piperine, an alkaloid from the pepper plant piper nigrum, for possible use in parkinson's disease.a series of compounds related to piperine and antiepilepsirine was screened in a monoamine oxidase a and b assay. piperine is an alkaloid from the source plant of both black and white pepper grains, piper nigrum. piperine has been shown to have a wide range of activity, including mao inhibitory activity. the z-factor for the screening assay was found to be greater than 0.8 for both assays. notably, the compounds tested were selective towards mao-b, with the most potent compound having an ic(50) ...201223102654
analysis of the blackening of green pepper (piper nigrum linnaeus) berries.this paper investigates polyphenol oxidase (ppo) activity, reduced weight percentage after sun drying, and the changes in colour and appearance of green pepper (piper nigrum linnaeus) berries after blanching and sun drying. the results show that the degree of reduced weight percentage and browning in green pepper berries after blanching for 10 min is greater at 100°c than at 90 and 80°c. moreover, the samples blanched at 100°c for 10 min had the fastest water loss, but the lowest ppo activity. t ...201223411179
synergistic larvicidal effect and morphological alterations induced by ethanolic extracts of annona muricata and piper nigrum against the dengue fever vector aedes aegypti.phytopesticide combinations of different botanical sources are seldom reported. annona muricata seed and piper nigrum fruit ethanolic extracts enriched in acetogenins and piperamides, respectively, were synergistically used as larvicides against the dengue fever vector aedes aegypti.201323112098
piper nigrum, piper betle and gnetum gnemon--natural food sources with anti-quorum sensing properties.various parts of piper nigrum, piper betle and gnetum gnemon are used as food sources by malaysians. the purpose of this study is to examine the anti-quorum sensing (anti-qs) properties of p. nigrum, p. betle and g. gnemon extracts. the hexane, chloroform and methanol extracts of these plants were assessed in bioassays involving pseudomonas aeruginosa pa01, escherichia coli [psb401], e. coli [psb1075] and chromobacterium violaceum cv026. it was found that the extracts of these three plants have ...201323519352
in vitro antibacterial and antibiotic-potentiation activities of four edible plants against multidrug-resistant gram-negative species.the present study was designed to investigate the antibacterial activities of the methanol extracts of four cameroonian edible plants, locally used to treat microbial infections, and their synergistic effects with antibiotics against a panel of twenty nine gram-negative bacteria including multi-drug resistant (mdr) phenotypes expressing active efflux pumps.201323885762
design, synthesis and trypanocidal evaluation of novel 1,2,4-triazoles-3-thiones derived from natural piperine.the work reported herein describes the synthesis and the assessment of the trypanocidal activity of thirteen new 1,2,4-triazole-3-thiones obtained from natural piperine, the main constituent of the dry fruits of piper nigrum. it is part of a research program aiming to use abundant and easily available natural products as starting materials for the design and synthesis of new molecules potentially useful as antiparasitic drugs. the variously substituted triazole derivatives were synthesized from ...201323760033
rapid detection of piper yellow mottle virus and cucumber mosaic virus infecting black pepper (piper nigrum) by loop-mediated isothermal amplification (lamp).the loop-mediated isothermal amplification (lamp) assay for piper yellow mottle virus and the reverse transcription (rt) lamp assay for cucumber mosaic virus each consisted of a set of five primers designed against the conserved sequences in the viral genome. both rna and dna isolated from black pepper were used as a template for the assay. the results were assessed visually by checking turbidity, green fluorescence and pellet formation in the reaction tube and also by gel electrophoresis. the a ...201323791964
ethnobotanical survey of malaria prophylactic remedies in odisha, investigate the plants traditionally used for prevention of malaria in cuttack, gajapati and koraput districts of odisha state, eastern india.201323434608
inactivation of escherichia coli o157:h7 and salmonella typhimurium in black pepper and red pepper by gamma irradiation.this study evaluated the efficacy of gamma irradiation to inactivate foodborne pathogens in black pepper (piper nigrum) and red pepper (dried capsicum annuum). black pepper and red pepper inoculated with escherichia coli o157:h7 and salmonella typhimurium were subjected to gamma irradiation in the range of 0, 1, 2, 3 and 5 kgy, and color change was evaluated after treatment. pathogen populations decreased with increasing treatment doses. a gamma irradiation dose of 5 kgy decreased e. coli o157:h ...201424370971
muscodor albus mow12 an endophyte of piper nigrum l. (piperaceae) collected from north east india produces volatile antimicrobials.muscodor albus mow12, an endophytic fungus isolated from piper nigrum in mawlong, meghalaya, india, resembles some cultural and hyphal characteristics of previous isolates of muscodor sp. in addition, it possesses about 99 % similarity in its its rdna with other m. albus isolates and thus is nicely centered within the genetic tree to other muscodor spp. this xylariaceae fungus effectively inhibits and kills certain plant pathogenic fungi by virtue of a mixture of volatile compounds that it produ ...201424426163
lc-ms/ms based identification of piperine production by endophytic mycosphaerella sp. pf13 from piper nigrum.piper nigrum is very remarkable for its medicinal properties due to the presence of metabolites like piperine. emerging understanding on the biosynthetic potential of endophytic fungi suggests the possibility to have piperine producing fungi in p. nigrum. in the current study, endophytic fungi isolated from p. nigrum were screened for the presence of piperine by liquid chromatography-tandem mass spectrometry (lc-ms/ms). this resulted in the identification of a mycosphaerella sp. with the ability ...201424691878
properties for sourcing nigerian larvicidal plants.aedes aegypti is the primary vector of chikungunya, yellow and dengue fevers. dengue fever is the major cause of child morbidity and hospitalisation in some asian and african countries, while yellow fever is prevalent in nigeria. the development of resistance to the available insecticides has necessitated the continued search for safer ones from plants. eighteen plant extracts with ethnomedical claims of or demonstrated febrifuge, antimalarial, insecticidal and insect repellent biological activi ...201424950440
studies on the factors modulating indole-3-acetic acid production in endophytic bacterial isolates from piper nigrum and molecular analysis of ipdc gene.the study mainly aimed quantitative analysis of iaa produced by endophytic bacteria under various conditions including the presence of extract from piper nigrum. analysis of genetic basis of iaa production was also conducted by studying the presence and diversity of the ipdc gene among the selected isolates.201424916921
isolation, characterization and identification of pericarp-degrading bacteria for the production of off-odour-free white pepper from fresh berries of piper nigrum isolate, fermentatively evaluate and identify black pepper (piper nigrum l.)-associated bacteria for the microbial decortication of fresh ripened berries and dried black pepper for preparation of off-odour-free white pepper.201424387754
piperine production by endophytic fungus colletotrichum gloeosporioides isolated from piper nigrum.many endophytic fungi have been reported with the biosynthetic potential to produce same or similar metabolites present in host plants. the adaptations that might have acquired by these fungi as a result of the long-term association with their host plants can be the possible basis of their biosynthetic potential. the bioactive compounds originated from endophytes are currently explored for their potential applications in pharmaceutical, agriculture and food industries. piper nigrum, a plant of t ...201424268806
protective effects of piperine against copper-ascorbate induced toxic injury to goat cardiac mitochondria in vitro.piperine, the main alkaloid of black pepper, piper nigrum linn., is an important indian spice used in traditional food and medicine in india. in the present study, we investigated the antioxidant activities of piperine against copper-ascorbate induced toxic injury to mitochondria obtained from a goat heart, in vitro. incubation of isolated cardiac mitochondria with copper-ascorbate resulted in elevated levels of lipid peroxidation and protein carbonylation of the mitochondrial membrane, a reduce ...201425059674
evaluation of in vitro aldose reductase inhibitory potential of alkaloidal fractions of piper nigrum, murraya koenigii, argemone mexicana, and nelumbo nucifera.aldose reductase is primarily involved in development of long-term diabetic complications due to increased polyol pathway activity. the synthetic aldose reductase inhibitors are not very successful clinically. therefore, the natural sources may be exploited for safer and effective aldose reductase inhibitors.201424127538
efficacy and safety of topical trikatu preparation in, relieving mosquito bite reactions: a randomized controlled trial.trikatu is composed of dried fruits of piper nigrum l and piper retrofractum vahl, and dried rhizomes of zingiber officinale r. although this preparation has been used to relieve pruritis, pain, and inflammation for a long time, there is no clinical evidence to confirm its efficacy and safety. therefore, we performed a double-blind, within person-randomized controlled study of 30 healthy volunteers to determine efficacy and safety of topical trikatu on mosquito bite reactions.201424559814
sequence diversity among badnavirus isolates infecting black pepper and related species in india.the badnavirus, piper yellow mottle virus (pymov) is known to infect black pepper (piper nigrum), betelvine (p. betle) and indian long pepper (p. longum) in india and other parts of the world. occurrence of pymov or other badnaviruses in other species of piper and its variability is not reported so far. we have analysed sequence variability in the conserved putative reverse transcriptase (rt)/ribonuclease h (rnase h) coding region of the virus using specific badnavirus primers from 13 virus isol ...201425674613
antifungal activity and computational study of constituents from piper divaricatum essential oil against fusarium infection in black pepper.fusarium disease causes considerable losses in the cultivation of piper nigrum, the black pepper used in the culinary world. brazil was the largest producer of black pepper, but in recent years has lost this hegemony, with a significant reduction in its production, due to the ravages produced by the fusarium solani f. sp. piperis, the fungus which causes this disease. scientific research seeks new alternatives for the control and the existence of other piper species in the brazilian amazon, resi ...201425375334
elucidation of the inhibitory effect of phytochemicals with kir6.2 wild-type and mutant models associated in type-1 diabetes through molecular docking approach.among all serious diseases globally, diabetes (type 1 and type 2) still poses a major challenge to the world population. several target proteins have been identified, and the etiology causing diabetes has been reasonably well studied. but, there is still a gap in deciding on the choice of a drug, especially when the target is mutated. mutations in the kcnj11 gene, encoding the kir6.2 channel, are reported to be associated with congenital hyperinsulinism, having a major impact in causing type 1 d ...201425705171
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