Publications

effect of co-administration of piperine on pharmacokinetics of beta-lactam antibiotics in rats.	co-administration of piperine, an alkaloid isolated from piper nigrum l. enhanced bioavailability of beta lactam antibiotics, amoxycillin trihydrate and cefotaxime sodium significantly in rats. the improved bioavailability is reflected in various pharmacokinetic parameters viz. tmax, cmax, t(1/2) and auc, of these antibiotics. the increased bioavailability could be attributed to the effect of piperine on microsomal metabolising enzymes or enzymes system.	2002	12635696
effect of piperine on the inhibition of lung metastasis induced b16f-10 melanoma cells in mice.	the effect of piperine on the inhibition of lung metastasis induced by b16f-10 melanoma cells was studied in c57bl/6 mice. simultaneous administration of the compound with tumor induction produced a significant reduction (95.2%) in tumor nodule formation. increased lung collagen hydroxyproline (22.37 microg/mg protein) in the metastasized lungs of the control animals compared to normal animals (0.95 microg/mg protein) was significantly reduced (2.59 microg/mg protein) in the piperine-treated ani ...	2002	12553376
aroma compound analysis of piper nigrum and piper guineense essential oils from cameroon using solid-phase microextraction-gas chromatography, solid-phase microextraction-gas chromatography-mass spectrometry and olfactometry.	the investigation of aroma compounds of the essential oils of dried fruits of black pepper (piper nigrum) and black and white "ashanti pepper" (piper guineense) from cameroon by means of solid-phase microextraction (spme) was carried out for the first time to identify the odorous target components responsible for the characteristic odor of these valuable spices and food flavoring products. by means of gc-flame ionization detection (fid) and gc-ms (using different polar columns) the main compound ...	2002	12462618
antimutagenic properties of bell and black peppers.	the wing somatic mutation and recombination test (smart) in drosophila melanogaster was used to study the modulating action of bell pepper (capsicum annuum) and black pepper (piper nigrum) in combination with the alkylating agent methyl methanesulfonate (mms) and the promutagen agent ethyl carbamate (ec). larvae trans-heterozygous for the third chromosome recessive markers multiple wing hairs (mwh) and flare-3 [flr(3)] were fed genotoxins alone or in combination with each of the two spices. gene ...	2003	12453727
protection of stored maize against sitophilus zeamais (motsch.) by use of essential oils of spices from cameroon.	in storage, maize as other cereals is currently attacked by insects. the main specie present in cameroon is sitophilus zeamais (coleop. curculionidae). the popular way to prevent its damages is the use of synthetic insecticides. these chemicals extend their activities to non target animals. they are potentially dangerous for consumers and are implicated in the pollution of the environment. the development of products killing pests which are consumer-friendly is to be improved. in this respect, t ...	2001	12425068
folklore information from assam for family planning and birth control.	the author collected folklore information on herbal treatments to control fertility from different parts of assam, india. temporary methods of birth control include cissampelos pareira l. in combination with piper nigrum l., root of mimosa pudica l. and hibiscus rosa-sinensis l. plants used for permanent sterilization include plumbago zeylanica l., heliotropium indicum l., salmalia malabrica, hibiscus rosa-sinensis l., plumeria rubra l., bambusa rundinacea. abortion is achieved through use of ...	1982	12266264
biochemical properties of polysaccharides from black pepper.	the purified polysaccharides from piper nigrum were prepared as follows: a hot water extract of pepper seeds was fractionated by ultrafiltration with a 5-kda-membrane cartridge. a fraction with 5 kda or bigger molecules was successively purified by open column chromatography on deae-toyopearl 650c and bio-gel p-60 with each active fraction, resulting in pn-ib and pn-iia, purified anti-complementary polysaccharides. none of the anti-complementary activity of any polysaccharide was changed by pron ...	2002	12230118
liquid chromatographic method for determination of piperine in rat plasma: application to pharmacokinetics.	piperine, a major alkaloid of piper longum and piper nigrum has been reported to have several pharmacological/toxicological effects. though a number of methods for analysis of this omnipresent food component in pepper fruits are available, its analysis in body fluids has been largely neglected. a high-performance liquid chromatography method for the analysis of piperine in rat plasma is presented in this communication. analysis was performed using a symmetry c(18) column (250x4.6 mm) by isocrati ...	2002	12138007
cyp3a4 inhibitory activity of new bisalkaloids, dipiperamides d and e, and cognates from white pepper.	two new bisalkaloids, dipiperamides d and e, were isolated as inhibitors of a drug metabolizing enzyme cytochrome p450 (cyp) 3a4 from the white pepper, piper nigrum. their structures were elucidated by spectroscopic methods. dipiperamides d and e showed potent cyp3a4 inhibition with ic(50) values of 0.79 and 0.12 microm, respectively, and other metabolites from the pepper were moderately active or inactive.	2002	12110320
piperine modulates permeability characteristics of intestine by inducing alterations in membrane dynamics: influence on brush border membrane fluidity, ultrastructure and enzyme kinetics.	piperine (1-piperoyl piperidine) is a major alkaloid of piper nigrum linn. and piper longum linn. it is shown to possess bioavailability-enhancing activity with various structurally and therapeutically diverse drugs. the mechanism of enhancing the bioavailability, is, however, not understood. we hypothesize that piperine's bioavailability-enhancing property may be attributed to increased absorption, which may be due to alteration in membrane lipid dynamics and change in the conformation of enzym ...	2002	12046863
larvicidal activity of isobutylamides identified in piper nigrum fruits against three mosquito species.	the insecticidal activity of materials derived from the fruits of piper nigrum against third instar larvae of culex pipiens pallens, aedes aegypti, and a. togoi was examined and compared with that of commercially available piperine, a known insecticidal compound from piper species. the biologically active constituents of p. nigrum fruits were characterized as the isobutylamide alkaloids pellitorine, guineensine, pipercide, and retrofractamide a by spectroscopic analysis. retrofractamide a was is ...	2002	11902925
[studies on the crude drugs used for the folk medicine described in "mimi-bukuro"].	"mimi-bukuro" is a book written by moriyasu negishi in the edo period. m. negishi (1737-1815) was a magistrate in the town of edo. he was very much interested in listening and recording many kinds of stories, which were told by the various kinds of people, such as public officers, samurais, merchants, doctors, etc. among the stories of this book, some stories were found concerning folk medicine, medicinal substance and charms. in this report, i studied the medicines which were used in these stor ...	1995	11613523
[essential oil analysis and trace element study of the roots of piper nigrum l].	pepper roots contain 0.39% of essential oil. by gc-ms method, 44 peaks and 22 compounds were obtained and identified. the major component was trans-caryophyllene. the trace metal content analysis showed that the heavy metals were under the safety level.	1998	11243157
tyrosinase inhibitory activity of bangladeshi indigenous medicinal plants.	the tyrosinase-inhibitory activity of 15 kinds of bangladeshi medicinal plants was evaluated. methanol extracts were prepared for screening tests, and other kinds of extracts were also studied for those with high activity. swertia chirata, piper nigrum, glycyrrhiza glabra, piper longam and ocimum americanum were screened as highly inhibiting samples. methanol was found to be the most efficient solvent for extracting the active compounds. the 50% tyrosinase-inhibitory concentration of the glycyrr ...	2000	11055404
novel procedure for identification of compounds inhibitory to transcription of genes involved in mycotoxin biosynthesis.	a novel assay is described for the identification and isolation of compounds that inhibit the transcription of genes involved in mycotoxin biosynthesis. the thin-layer chromatography-based assay was used to screen plant extracts for compounds that would inhibit the expression of the beta-glucuronidase reporter gene under the control of an aflatoxin biosynthesis gene promoter in aspergillus parasiticus. the assay was used to track purification of an inhibitory compound, cp2, from extracts of blac ...	2000	11052714
influence of intragastric perfusion of aqueous spice extracts on acid secretion in anesthetized albino rats.	the effect of spices on gastric acid secretion is variable. their mechanism of action is also not well established.	2000	10812814
antimicrobial agents from plants: antibacterial activity of plant volatile oils.	the volatile oils of black pepper [piper nigrum l. (piperaceae)], clove [syzygium aromaticum (l.) merr. & perry (myrtaceae)], geranium [pelargonium graveolens l'herit (geraniaceae)], nutmeg [myristica fragrans houtt. (myristicaceae), oregano [origanum vulgare ssp. hirtum (link) letsw. (lamiaceae)] and thyme [thymus vulgaris l. (lamiaceae)] were assessed for antibacterial activity against 25 different genera of bacteria. these included animal and plant pathogens, food poisoning and spoilage bacte ...	2000	10736000
comparison of digestion methods for determination of trace and minor metals in plant samples.	in this paper, three dissolution methods using pressure digestion vessels (low-, medium-, and high-pressure vessels) for the determination of metals in plant samples are described. the plackett-burman saturated factorial design was used to identify the significant factors influencing wet ashing and to select optimized dissolution conditions. the three methods were statistically compared (on-way anova) on the same sample; no significant differences were obtained. in all cases the relative standar ...	1999	10606575
stimulation of mouse melanocyte proliferation by piper nigrum fruit extract and its main alkaloid, piperine.	during a herbal screening programme to find potential repigmenting agents for the treatment of vitiligo, piper nigrum l. fruit (black pepper) extract was found to possess growth-stimulatory activity towards cultured melanocytes. its aqueous extract at 0.1 mg/ml was observed to cause nearly 300% stimulation of the growth of a cultured mouse melanocyte line, melan-a, in 8 days (p < 0.01). piperine (1-piperoylpiperidine), the main alkaloid from piper nigrum fruit, also significantly stimulated mela ...	1999	10575373
jamu gendong, a kind of traditional medicine in indonesia: the microbial contamination of its raw materials and endproduct.	an examination on the microbiological quality of seven kinds of jamu gendong (jg) and their raw materials has been conducted according to the requirements of microbial contamination in traditional medicine, issued by the department of health of indonesia in 1986. samples of jg and their raw materials were taken from producers in three districts of surabaya. the samples were subject to the following examinations: total plate count (tpc), mpn coliform, the enumeration of molds and yeasts, the pres ...	1998	10030724
influence of spices on the bacterial (enzyme) activity in experimental colon cancer.	in the presence of a known colon carcinogen, 1,2-dimethyl hydrazine (dmh), the activity of beta-glucuronidase was found to be significantly increased in the distal colon, distal intestine, liver and colon contents and the activity of mucinase was increased in both the colon and fecal contents when compared to control rats. chilli (capsicum annum l., solanaceae) administration also showed an increase when compared to control rats, whereas supplementation with cumin (cuminum cyminum l., apiaceae) ...	1998	9720607
permeability characteristics of piperine on oral absorption--an active alkaloid from peppers and a bioavailability enhancer.	piperine, [1-[5-[1,3-benzodioxol-5-yl]-1-oxo-2,4, pentadienyl] piperidine], is a pungent alkaloid present in piper nigrum linn, and p. longum linn. it is shown to enhance the bioavailability of various structurally and therapeutically diverse drugs. a concise mechanism responsible for its bioavailability enhancing action is poorly understood. this study is an effort to understand the absorption dynamics of piperine in intestine on oral absorption. it encompasses intestinal everted sacs as an exp ...	1998	9536651
high incidence of aspergillus flavus and aflatoxins in stored groundnut in ghana and the use of a microbial assay to assess the inhibitory effects of plant extracts on aflatoxin synthesis.	groundnut samples from 21 selected markets in the 10 regions of ghana yielded high levels of the aflatoxigenic fungus aspergillus flavus on half-strength potato dextrose agar. the fungus was associated with 31.7 and 12.8%, respectively, of all damaged and undamaged kernels assayed. only 0.24% of total kernels assayed yielded a. parasiticus. other fungi detected from total kernels assayed were a. niger (34%), a. candidus (1.45%), a. tamarii (3.93%), a. ochraceous (5.26%), fusarium spp. (1.7%) pen ...	1996	8981776
evaluation of the modulatory influence of black pepper (piper nigrum, l.) on the hepatic detoxication system.	the present paper assesses the modifying potential of black pepper on the hepatic biotransformation system in mice. the modulatory effect was assessed on glutathione s-transferase (gst), cytochrome b5 (cyt. b5), cytochrome p-450 (cyt. p-450), acid-soluble sulfhydryl (-sh) content and malondialdehyde (mda) level. swiss albino mice of either sex (eight weeks old) were fed on a diet containing 0.5%, 1% and 2% black pepper (w/w) for 10 and 20 days. the findings revealed a significant and dose-depend ...	1993	8402574
common indian spices: nutrient composition, consumption and contribution to dietary value.	nutrient composition of eight commonly consumed spices of south india was analysed. spices analysed were red chillies (capsicum annum), black pepper (piper nigrum), coriander seeds (coriandrum sativum), cumin seeds (cuminum cyminum), garlic (allium sativum), asafoetida (ferula foetida), dry ginger (zingiber officinale) and ajowan (carum copticum). the nutrients analysed were proximate principles, minerals, starch, sugars, dietary fibre components, tannins, phytic acid, enzyme inhibitors and amin ...	1993	8397396
evaluation of the liver protective potential of piperine, an active principle of black and long peppers.	piperine, an active alkaloidal constituent of the extract obtained from piper longum and piper nigrum, was evaluated for its antihepatotoxic potential in order to validate its use in traditional therapeutic formulations. this plant principle exerted a significant protection against tert-butyl hydroperoxide and carbon tetrachloride hepatotoxicity by reducing both in vitro and in vivo lipid peroxidation, enzymatic leakage of gpt and ap, and by preventing the depletion of gsh and total thiols in th ...	1993	8255933
resiniferatoxin and piperine: capsaicin-like stimulators of oxygen uptake in the perfused rat hindlimb.	the naturally occurring capsaicin-like molecules, resiniferatoxin (rtx, euphorbia spp.) and piperine (piper nigrum), each stimulated oxygen uptake (vo2) in association with increased vascular resistance in a concentration-dependent manner when infused into the perfused rat hindlimb. 5 microm glyceryl trinitrate (gtn, a nitrovasodilator) significantly blocked the oxygen and pressure responses to both rtx and piperine, indicating a close relationship between changes in vo2 and the vasoconstriction ...	1994	8035653
antibacterial activity of alimentary plants against staphylococcus aureus growth.	alimentary plants were screened for antibacterial activity against a penicillin g resistant strain of staphylococcus aureus. twenty-five samples of plant material corresponding to 21 species from 13 families were used. both aqueous and ethanol extracts were obtained from them. antibacterial activity was determined by the agar-well diffusion method, using cephazolin as a standard antibiotic. seventeen ethanol extracts were found active. eugenia caryophyllata (clavo de olor*) flowers, myristica fr ...	1994	7992816
influence of spices on protein utilisation of winged bean (psophocarpus tetragonolobus) and horsegram (dolichos biflorus).	the influence of a mixture of eleven spices commonly consumed in india on the utilisation of protein from boiled winged bean (psophocarpus tetragonolobus) and horsegram (dolichos biflorus) was studied at 10 and 20 percent level of protein intake in experimental rats. spices used in the mixture include red chillies (capsicum annum), black pepper (piper nigrum), coriander (coriandrum sativum), cumin (cuminum cyminum), garlic (allium sativum), ajowan (carum copticum), turmeric (curcuma longa), cara ...	1994	7855088
determination of piperine in pepper (piper nigrum) using high-performance liquid chromatography.		1983	6885950
fungus flora of black and white pepper (piper nigrum l.).		1973	4517377
[decontamination of spices by ethylene oxide. the case of cloves (eugenia caryophyllus spreng.) and white pepper (piper nigrum l.)].		1985	4062177
chemical constituents of peppers (piper spp.) and application to food preservation: naturally occurring antioxidative compounds.	in a structure analysis of the compounds of the genus piper (family piperaceae), we identified five phenolic amides from piper nigrum, seven compounds from p. retrofractum, and two compounds from p. baccatum. all the phenolic amides possess significant antioxidant activities that are more effective than the naturally occurring antioxidant, alpha-tocopherol. one amide, feruperine, has antioxidant activity as high as the synthetic antioxidants, butylated hydroxyanisole (bha) and butylated hydroxyt ...	1986	3757949
effect of black pepper and piperine on bile secretion and composition in rats.	the influence of black pepper (piper nigrum l.) and its active principle, piperine on the secretion and composition of bile was investigated in the rat. they were administered by gavage (black pepper at 250 or 500 mg and piperine at 12.5 or 25 mg/kg body wt.) or fed in the diet for 4 weeks (black pepper at 0.2 and 0.4%, piperine at 0.01 and 0.02%). the lower dose by gavage of black pepper caused an increase in bile solids while with other treatments bile secretion or dry matter in bile was not c ...	1987	3431580
carcinogenicity testing of black pepper (piper nigrum) using the egyptian toad (bufo regularis) as a quick biological test animal.	milled black pepper (piper nigrum) force-fed to egyptian toads as a suspension in amphibian saline or injected subcutaneously in the dorsal lymph sac as an ethanol extract, induced primary tumours in the liver and secondary tumours in other organs (kidney and spleen). when applied to the skin of experimental animals as an ethanol extract, black pepper induced primary tumours in the liver and secondary tumours in the ileum and stomach. tumours of the liver were diagnosed as hepatocellular carcino ...	1988	3368202
aflatoxin contamination of some common drug plants.	this paper deals with the natural occurrence of aflatoxins in some common drug plants collected from storehouses in bihar, india. of 15 samples analyzed, 14 were aflatoxin positive. the highest level of aflatoxin contamination was detected in the seeds of piper nigrum (1.20 micrograms/g), followed by the level detected in the seeds of mucuna prurita (1.16 micrograms/g), and the lowest level was detected in the bark of acacia catechu (0.09 micrograms/g). of 158 isolates of aspergillus flavus obta ...	1988	3132102
effect of piperine on pentobarbitone induced hypnosis in rats.	piperine (1-peperoyl piperidine), a major alkaloid isolated from piper nigrum linn, potentiated pentobarbitone sleeping time in dose dependant manner, with peak effect at 30 min. blood and brain pentobarbitone levels were higher in piperine treated animals. piperine treatment in rats, treated chronically with phenobarbitone, significantly potentiated pentobarbitone sleeping time, as compared to the controls. there was no alteration in barbital sodium sleeping time. it is possible that, piperine ...	1990	2401524
anti-inflammatory activity of piperine.	piperine (1-peperoyl piperidine) was isolated from piper nigrum linn for the evaluation of anti-inflammatory activity in rats. different acute and chronic experimental models like carrageenin-induced rat paw edema, cotton pellet granuloma, and croton oil-induced granuloma pouch, were employed. simultaneously, biochemical estimations were made to elucidate the underlying mechanism of the action. piperine acted significantly on early acute changes in inflammatory processes and chronic granulative ...	1990	2283727
carcinogenesis induced by black pepper (piper nigrum) and modulated by vitamin a.	painting and feeding of mice with 2mg of an extract from black pepper on 3 days a week for 3 months results in a significant increase of the number of tumor-bearing mice. tumor incidence is reduced in those groups of experimental animals receiving 5 or 10mg vitamin a-palmitate twice weekly for 3 months by feeding or painting during and subsequent to application of pepper extract. feeding of mice with powder of black pepper in diet (50g/3kg food) has no impact on carcinogenesis.	1990	2098270
influence of spices on utilization of sorghum and chickpea protein.	influence of eight common indian spices on the protein quality of sorghum and chickpea was studied. spices used include red chillies (capsicum annum), black pepper (piper nigrum), coriander seeds (coriandrum sativum) cumin seeds (cuminum cyminum), garlic (allium sativum), asafoetida (ferula foetida), dry ginger (zingiber officinale) and ajowan (carum copticum). addition of spices did not affect protein digestibility (td) of sorghum. the bv of all sorghum diets with spices was higher than that of ...	1991	1924191
carcinogenic effect of force-feeding an extract of black pepper (piper nigrum) in egyptian toads (bufo regularis).	50 male and 50 female bufo regularis were treated, by force-feeding, with an extract of black pepper, at a dose level of 2 mg, 3 times a week for 5 months. the first tumors appeared after 2 months. liver tumors (hepatocellular carcinomas, lymphosarcomas and fibrosarcomas) were found in 12 males and 18 females. metastatic deposits of hepatocellular carcinomas were registered in the spleen, kidney, fat body and ovary.	1991	1891179
carcinogenicity testing of some constituents of black pepper (piper nigrum).	in mice, injection of safrole, tannic acid or methylcholanthrene (mca) during the preweaning period induced tumors in different organs. safrole and tannic acid (constituents of black pepper) were weak carcinogens when compared with mca which was used as a carcinogenic control substance. force feeding of d-limonene (one of the pepper terpenoids) for a long time to the mice which were injected with any of the above 3 substances reduced their carcinogenic activity, while force feeding of piperine ( ...	1992	1617288
piperine-mediated changes in the permeability of rat intestinal epithelial cells. the status of gamma-glutamyl transpeptidase activity, uptake of amino acids and lipid peroxidation.	the effect of piperine (1-[5-(1,3-benzodioxol-5-yl)-1-oxo-2,4-pentadienyl]piperidin e), (from piper nigrum) on the absorptive function of the intestine was studied. in vitro experiments showed that piperine (25-100 microm) significantly stimulated gamma-glutamyl transpeptidase (gamma-gt, ec 2.3.2.2.) activity, enhanced the uptake of radiolabelled l-leucine, l-isoleucine and l-valine, and increased lipid peroxidation in freshly isolated epithelial cells of rat jejunum. the kinetic behaviour of ga ...	1992	1348936
occurrence of n-isobutyl eicosa-trans-2-trans-4-dienamide in piper nigrum.		1976	981396
incidence of aflatoxin-potential contamination in spanish sausages.	525 strains of moulds were isolated from 8 samples of red pepper (capsicum annuum) and from 3 samples of black pepper (piper nigrum). these spices were commonly added to the various kinds of spanish sausages, specially to the spanish "chorizo". of these 525 isolated moulds, 100 belonged to the aspergillus flavus group. 69 were isolated from red pepper and 31 from black pepper. of these strains, 28 strains isolated from red pepper were found positive in producing aflatoxin b1 while only 8 of the ...	1977	613913