TitleAbstractYear(sorted ascending)
identification of a tenth mrna of respiratory syncytial virus and assignment of polypeptides to the 10 viral genes.nine mrnas, their cdna clones, and a genome transcriptional map have been reported previously for respiratory syncytial virus (p. l. collins and g. w. wertz, proc. natl. acad. sci. u.s.a. 80:3208-3212, 1983). we report here the identification of a 10th viral mrna, designated mrna 2b (molecular weight [mw] ca. 0.39 x 10(6)), that was detected by rna (northern) blot hybridization with cdna clones. analysis of a polycistronic readthrough transcript was used to deduce the position in the viral trans ...19846546401
sequence analysis of the respiratory syncytial virus phosphoprotein gene.a recombinant cdna plasmid (prsa3) containing an almost full-length copy of the mrna encoding respiratory syncytial virus phosphoprotein was identified in a cdna library prepared with mrna from respiratory syncytial virus-infected cells. the cdna insert was sequenced, and a protein of 27,150 daltons was deduced from the dna sequence. the protein is relatively acidic, containing two clusters of acidic amino acids, one in the middle of the molecule and the other at the c-terminus. it is devoid of ...19846548527
giant cell pneumonia due to respiratory syncytial virus. occurrence in severe combined immunodeficiency syndrome.a 6-month-old male infant with a severe combined immunodeficiency syndrome was hospitalized for progressive respiratory distress. examination during hospitalization disclosed widespread pulmonary infiltrates that did not respond to intensive therapy. the patient died eight days after admission. autopsy disclosed pneumocystis carinii pneumonia and widespread giant cell pneumonia. respiratory syncytial virus (rsv) was grown from a lung specimen obtained at autopsy. specific immunofluorescent stain ...19846611144
cytotoxic t-cell response to respiratory syncytial virus in mice.the role of the humoral and cellular arms of the immune response in protection against respiratory syncytial virus (rsv) infection and in the pathogenesis of the severe forms of this disease is poorly understood. the recent demonstration that some inbred mouse strains can be infected with rsv has opened the way to a detailed investigation of rsv immunity. we report here the finding of major histocompatibility complex-restricted, rsv-specific memory cytotoxic t cells in the spleens of balb/c and ...19852411951
two distinct subtypes of human respiratory syncytial virus.antigenic variation of human respiratory syncytial (rs) virus strains was analysed using a collection of nine, six, six, nine and one monoclonal antibodies respectively directed against the large glycoprotein (g), fusion protein (f), matrix protein (m), nucleoprotein (np) and phosphoprotein (p) components of the long strain of rs virus. a comparison was made with seven other strains isolated during different years in radioimmune precipitation analyses and immune fluorescence tests. two different ...19852413163
respiratory syncytial virus infection in transported calves.nasal swab samples were collected from calves on individual farms in tennessee and sequentially at an auction barn and at a feedlot to detect respiratory syncytial virus (rsv). in 1976, rsv was isolated from 5 of 225 calves at the auction barn and from 13 of 92 calves examined at the feedlot. of the 13 isolations, 11 were from calves with acute respiratory tract disease. most (14/18) calves infected with rsv were also shedding parainfluenza-3 virus in their nasal secretions. attempts to isolate ...19852417510
clinical applications of antiviral agents for chemophrophylaxis and therapy of respiratory viral infections.table iii summarizes clinical applications of antiviral agents in respiratory viral infections. (table: see text) for influenza a virus infections, both oral amantadine and rimantadine are effective when used for seasonal prophylaxis and for prophylaxis in institutional populations. both of these drugs, as well as aerosolized ribavirin, have antiviral and therapeutic effects in uncomplicated influenza. it remains to be determined whether any of these modalities or possibly their combined use [44 ...19852417551
the disinfection of respiratory syncytial virus by isopropanol and a chlorhexidine-detergent handwash.two of the products commonly used to disinfect the hands of nursing staff are 70% isopropanol and aqueous chlorhexidine-detergent solutions. these have been shown to rapidly destroy respiratory syncytial virus, and are therefore suitable for use where contagious spread of this virus is suspected. the virucidal component of the chlorhexidine-detergent preparation has been shown to be primarily the detergent base and not the chlorhexidine.19852859328
monoclonal antibodies for the rapid diagnosis of influenza a and b virus infections by immunofluorescence.mouse monoclonal antibodies, directed against antigenic sites on influenza a and b viruses and found to be type-specific in an immunoassay, were assessed for use as diagnostic reagents in an indirect immunofluorescence assay on nasopharyngeal secretions. the influenza a antibodies were directed against nucleoprotein or matrix protein antigens and the influenza b antibodies against nucleoprotein and haemagglutinin antigens. the influenza a anti-matrix monoclonal antibody was found to give a stron ...19852865418
cytotoxic lymphocytes in the lungs of mice infected with respiratory syncytial virus.mice infected with respiratory syncytial virus (rsv) developed cytotoxic lymphocytes in the lungs, which lysed rsv-infected, but not uninfected cells. cytotoxic activity was greatest 7 to 9 days after infection, was virus-specific, mhc-restricted and abolished by treatment of lymphocytes with anti-thy 1.2 or with anti-lyt 2.2 sera and complement. there was a close temporal relationship between the appearance of these cytotoxic lymphocytes in the lung and clearance of virus. in contrast, rsv pers ...19852866227
[natural killers and interferon in complicated influenza and other acute respiratory viral diseases in children].the results of the study on the functional activity of natural killers (nk) and serum interferon in 118 children ranging in age from 3 months to 5 years suffering from influenza and other acute respiratory viral diseases complicated with croup, pneumonia, and obstructive syndrome (66 children with influenza, 21 with parainfluenza, 16 with adenovirus infection, and 15 with respiratory syncytial virus diseases) are presented. the control group consisted of 22 normal children. decreased nk activity ...19852420069
antiviral effect of flavonoids on human viruses.the effect of several naturally occurring dietary flavonoids including quercetin, naringin, hesperetin, and catechin on the infectivity and replication of herpes simplex virus type 1 (hsv-1), polio-virus type 1, parainfluenza virus type 3 (pf-3), and respiratory syncytial virus (rsv) was studied in vitro in cell culture monolayers employing the technique of viral plaque reduction. quercetin caused a concentration-dependent reduction in the infectivity of each virus. in addition, it reduced intra ...19852981979
identification of respiratory virus antigens in middle ear fluids of children with acute otitis media.antigens of respiratory viruses were tested by immunoassay of the middle ear fluids and nasopharyngeal secretions of 137 children with acute otitis media. the following were found: (1) an epidemic of infection with respiratory syncytial virus (rsv) caused a significant increase in the occurrence of acute otitis media. (2) fifteen percent of the children had rsv antigens in middle ear fluid, and in 7% rsv was the sole pathogen found. adenovirus antigens were found in the middle ear fluid of 3% of ...19852982961
antiviral chemotherapy and chemoprophylaxis.antiviral compounds have been developed for use in chemoprophylaxis and chemotherapy of a variety of infections in humans, including those caused by influenza viruses, respiratory syncytial virus, and herpesviruses. the efficacy of several of these compounds has been demonstrated in rigorously controlled trials. advances in molecular virology have led to the identification of biochemically defined, virus-specific functions that serve as appropriate targets for the future development of antiviral ...19852983421
detection of viral and chlamydial antigens in open-lung biopsy specimens.the recovery of viruses and chlamydia trachomatis from cell cultures and the detection of their antigens in impression smears prepared from open-lung biopsy (olb) specimens from immunocompromised adults were compared. touch impression smears were prepared on three slides, each containing eight wells. olb tissue was homogenized (stomacher) and inoculated into mrc-5, primary monkey kidney, and mccoy cell cultures. the direct and indirect immunofluorescence (if) tests were used to detect antigens t ...19852983526
respiratory syncytial virus fusion glycoprotein: nucleotide sequence of mrna, identification of cleavage activation site and amino acid sequence of n-terminus of f1 subunit.the amino acid sequence of respiratory syncytial virus fusion protein (fo) was deduced from the sequence of a partial cdna clone of mrna and from the 5' mrna sequence obtained by primer extension and dideoxysequencing. the encoded protein of 574 amino acids is extremely hydrophobic and has a molecular weight of 63371 daltons. the site of proteolytic cleavage within this protein was accurately mapped by determining a partial amino acid sequence of the n-terminus of the larger subunit (f1) purifie ...19852987829
serum antibody to respiratory syncytial virus in goats in the uk. 19852988182
surveillance of respiratory viral infections by rapid immunofluorescence diagnosis, with emphasis on the epidemiological development of respiratory syncytial virus infections.surveillance of certain respiratory viral infections by applying immunofluorescence (if) examinations to samples of nasopharyngeal secretions has been evaluated using a simplified procedure for the preparation of cell smears. samples from 711 children living in different parts of norway were examined during the winter 1982/83 and a positive diagnosis was made for 290 children (41%). temporal epidemic peaks were observed for respiratory syncytial virus (rsv), parainfluenza virus type 3 and influe ...19852989359
acute respiratory tract infections of children in hospital: a viral and mycoplasma pneumoniae profile.respiratory specimens and blood were collected from all infants and children admitted with acute respiratory illness to a paediatric unit in christchurch from may to november (late autumn, winter and spring) 1983, to define the viral aetiological agents involved. a virus or mycoplasma pneumoniae was identified in 160 (50%) of 317 children studied by the rapid indirect immunofluorescence, virus culture and/or serological techniques. aetiological agents were detected in 71% of children with bronch ...19852991830
a viral antigen-bearing cell line derived from culture of paget's bone cells.this study documents the characteristics of a viral antigen-bearing cell line derived from co-culture of the bone from a 71-year-old woman with paget's disease of bone and hep-2 cells. the cell line has survived in continuous culture for 3 1/2 years and 185 subcultures. the cells are epithelioid in appearance, produce alkaline and acid phosphatase, increase alkaline phosphatase activity in response to 1,25-(oh)2-d3 and contain receptors for 1,25-(oh)2-d3. immunofluorescent studies utilizing anti ...19852996574
nucleotide sequences of the 1b and 1c nonstructural protein mrnas of human respiratory syncytial virus.the genes encoding the 1c and 1b mrnas of human respiratory syncytial (rs) virus are first in the order of viral transcription and encode nonstructural (ns) proteins of approximate molecular weights 14,000 and 11,000, respectively, estimated by gel electrophoresis. the complete nucleotide sequences of the 1c and 1b mrnas determined from several full-length cdna clones are described. the 1c and 1b mrnas contain 528 and 499 nucleotides, respectively, exclusive of poly(a), and encode proteins of 13 ...19852998021
persistence of viruses in the nasopharynx of apparently healthy children aged 0-5 years. results of investigations performed in 1982-83.virus isolation attempts were made with nasopharyngeal secretions from 400 apparently healthy 0-5-year-old children of a semi-closed community. repeated virus isolations were achieved in 25 cases subjected to periodic investigations in 1982-83. one-two reisolation(s), 1-5 months apart, of the initially detected viral agents could be obtained in 15 out of the 25 children. the following viruses were reisolated (in decreasing order of frequency): adenoviruses, respiratory syncytial virus, coxsackie ...19853000066
a simple method for increased recovery of purified paramyxovirus virions.a simple method involving vigorous agitation of infected cell monolayers prior to collection of culture medium is described to greatly increase the recovery of purified virions of measles virus, respiratory syncytial virus and human parainfluenza virus. vigorous agitation of the flasks containing monolayers of infected cells increased the recovery of purified virions by at least 3- to 10-fold as judged by the intensity of [35s]methionine labeled viral proteins on sodium dodecyl sulphate-polyacry ...19853001119
production of monoclonal antibodies against parainfluenza 3 virus and their use in diagnosis by immunofluorescence.monoclonal antibodies were produced against parainfluenza virus type 3 (pi-3) and used to identify pi-3 clinical isolates in cell culture and pi-3 antigen in cells obtained from nasopharyngeal (np) washes of patients. two (2e9 and 4g5) of the three monoclonal antibodies characterized reacted by immunoblotting with a 67,000-dalton pi-3 protein, and one antibody (4e5) reacted with two viral proteins in the range of 29,000 to 31,000 daltons. the three monoclonal antibodies did not cross-react by in ...19853001131
a survey of respiratory syncytial virus and parainfluenza virus type 3 neutralising and immunoprecipitating antibodies in relation to paget disease.the aetiology of paget disease of bone has not been established but certain features have suggested involvement of a parainfluenzalike virus. to seek further evidence of the possible role of paramyxoviruses in paget disease we have surveyed the presence of neutralising and immunoprecipitating antibodies to both respiratory syncytial virus and parainfluenza virus type 3 in the sera of patients attending a bone disease clinic. these two viruses were implicated by the sporadic observation of viral ...19853001223
seroepidemiologic survey for antibodies to selected viruses in the respiratory tract of lambs.a serologic study was conducted to determine the prevalence of antibodies to, and infection rate of, mastadenovirus ovi 5, m ovi 6, parainfluenza-3 (pi-3) virus, bovine herpesvirus-1 (bhv-1), respiratory syncytial virus (rsv), bovine viral diarrhea (bvd) virus, and ovine progressive pneumonia (opp) virus in lambs at a ram lamb growth-rate test station. for 2 consecutive years, serum samples were prepared from blood collected from 1- to 2-month-old ram lambs as they entered the test station (1st ...19853002217
the effects of acute respiratory virus infection upon tracheal mucous transport.tracheal mucous velocity was measured in 13 healthy non-smokers using a radioisotope-labeled aerosol and a multidetector probe during respiratory virus infections. the movement of boluses of tracheal mucous were either absent or reduced in number in five subjects with myxovirus infection (four influenza and one respiratory syncytial virus) within 48 hr of the onset of symptoms and in four subjects 1 wk later. one subject with influenza still had reduced bolus formation 12-16 wk after infection. ...19853002287
[method of spread of respiratory syncytial virus infection in a neonatology unit]. 19853157115
[respiratory syncytial virus infection, with special reference to recent findings]. 19852422814
antigenic characterization of respiratory syncytial virus strains with monoclonal study the antigenic characteristics of respiratory syncytial virus (rsv), we developed and evaluated monoclonal antibodies (mabs) to three strains of rsv: 11 to long, 4 to 18537, and 9 to a2. six of these mabs immunoprecipitated the nucleoprotein, six the large glycoprotein, and 11 the fusion protein. by the pattern of the reactions of these mabs to 16 strains of rsv in an indirect immunofluorescence assay or enzyme-linked immunosorbent assay, we were able to distinguish three subgroups. with ...19852579169
antigenic analysis of a putative new strain of respiratory syncytial virus.a recent isolate (9320) of respiratory syncytial virus (rsv) that had been reported to differ from all previous strains was compared with rsv strains isolated over a period of 23 years by using the techniques of in vitro neutralization by specific antiserum and in vivo resistance to infection in cotton rats. the 9320 strain did not appear to be a novel antigenic variant and exhibited greater homology to several prototype strains than did an earlier isolate. the antigenic differences detected by ...19852579170
purification and characterization of the respiratory syncytial virus fusion protein.the fusion protein of respiratory syncytial virus was purified by affinity chromatography using a monoclonal antibody. under various conditions the protein was recovered as a 145k dimer or a 70k monomer. the 70k monomer was composed of disulphide-linked fragments of 48k and 23k. polyclonal rabbit serum produced to the dimerized fusion protein neutralized virus but did not inhibit fusion, while rabbit serum to the 2-mercaptoethanol-treated dimerized protein neutralized virus and inhibited fusion ...19853838336
the envelope-associated 22k protein of human respiratory syncytial virus: nucleotide sequence of the mrna and a related polytranscript.we recently determined that respiratory syncytial virus (strain a2) encodes a fourth unique envelope-associated virion protein that has molecular weight of approximately 24,000, as estimated by gel electrophoresis. the nucleotide sequence of the mrna encoding this novel protein has now been determined from five cdna clones, including three that contain the complete mrna sequence. the complete mrna sequence is 957 nucleotides, exclusive of polyadenylate, and contains two partially overlapping ope ...19853838351
giant cell pneumonia due to respiratory syncytial virus. 19853838449
nucleotide sequence of the respiratory syncytial virus phosphoprotein gene.a cdna library was prepared from poly(a)+ rna extracted from respiratory syncytial (rs) virus-infected hep-2 cells. a recombinant plasmid, prsp68, encoding the rs virus phosphoprotein gene was identified by translation in vitro of hybrid-selected mrna. the cloned cdna insert of 1 kb was sequenced and a polypeptide of 241 amino acids with a molecular weight of 27 166 was deduced from the sequence. the protein was relatively rich in polar amino acids and devoid of both cysteine and tryptophan. a s ...19853839520
correct sequence for the major nucleocapsid protein mrna of respiratory syncytial virus.a nucleotide sequence for the mrna of the major nucleocapsid (n) protein gene of respiratory syncytial virus was reported previously (n. elango and s. venkatesen, 1983, nucleic acids res. 11, 5941-5951). however, we have been unable to confirm part of this sequence as n mrna-specific and suggest that the published sequence represents that of an aberrant chimeric transcript. here we present an alternative sequence for the n mrna and provide data supporting its authenticity. the corrected n mrna s ...19853839952
nucleotide sequence of the g protein gene of human respiratory syncytial virus reveals an unusual type of viral membrane protein.the major surface glycoprotein (g) of human respiratory syncytial (rs) virus has an estimated mature mr of 84,000-90,000. among a library of cdna clones prepared from rs virus mrnas, we identified clones that hybridized to a message that encoded a mr 36,000 polypeptide that was specifically immunoprecipitated with anti-g antiserum. the amino acid sequence of the g protein backbone was determined by nucleotide sequence analysis of several of the cdna clones. it contains a combination of structura ...19853858865
respiratory syncytial virus and heart block--cause and effect?a child aged 3 years who developed a respiratory syncytial virus infection is described. his admission was complicated by the development of an irregular pulse. he initially had a variable first or second degree heart block with transient electrocardiographic evidence suggestive of pericarditis and/or myocarditis and later developed complete heart block. he has remained asymptomatic for the past four years. a search for other causes of the heart block proved negative. it is tempting to suggest a ...19853859266
fatal respiratory syncytial virus infection in severe combined immunodeficiency syndrome.we encountered two cases of fatal respiratory syncytial virus infection in infants. both patients had severe anatomic abnormalities in the thymus, probably due to severe combined immunodeficiency syndrome.19853877455
seroprevalence of respiratory syncytial virus in free-ranging bighorn sheep.sera from 187 (42%) of 447 free-ranging bighorn sheep (ovis canadensis) in 9 western states had antibody titers to respiratory syncytial virus (rsv). titers were from 1:5 to 1:640, as determined by the standard virus neutralization test. results of the survey indicated that rsv is widespread in free-ranging bighorn sheep populations and that rsv may be an important factor in the bighorn sheep pneumonia-complex.19853878355
survey of desert bighorn sheep in california for exposure to selected infectious diseases.from february 1983 to june 1985, 188 desert bighorn sheep (ovis canadensis nelsoni, = 161 and oc cremnobates, = 27) from 18 herds in 17 mountain ranges and one captive herd were caught, marked, and had blood, fecal, and nasal mucus samples collected. nasal swab specimens were cultured bacteriologically and virologically specifically for parainfluenza-3 (pi-3) virus. bacterial flora differed from herd to herd. pathogenic pneumophilic bacteria (eg, pasteurella sp) seldom were found. parainfluenza- ...19853878356
the 1a protein gene of human respiratory syncytial virus: nucleotide sequence of the mrna and a related polycistronic transcript.the 1a mrna is the smallest mrna of human respiratory syncytial (rs) virus and encodes a single protein of approximate molecular weight 9500 as estimated previously by gel electrophoresis. the nucleotide sequence of the 1a mrna, determined from several full-length cdna clones, is reported. the 1a mrna consists of 405 nucleotides, exclusive of poly(a), with relatively long nontranslated regions at the 5' and 3' ends (84 and 126 nucleotides, respectively). the sequences at the 5' and 3' termini of ...19853879976
monoclonal antibodies against respiratory syncytial virus and their use for rapid detection of virus in nasopharyngeal secretions.we developed five monoclonal antibodies against respiratory syncytial virus. three of these (23a3, 12a4, and 18b2) were used in an indirect fluorescent antibody test, and the results were compared with those of a similar indirect fluorescent test with commercial anti-respiratory syncytial virus serum. the results obtained with antibody 18b2 and commercial anti-respiratory syncytial virus serum were identical, whereas with antibodies 23a3 and 12a4 the incidence of positive identifications was aro ...19853882752
the development of monoclonal antibodies to respiratory syncytial virus and their use in diagnosis by indirect immunofluorescence.twelve clones of murine hybridoma cells secreting antibody specific for respiratory syncytial (rs) virus were classified into four groups on the basis of their pattern of staining of unfixed rs virus-infected hep-2 cells in an indirect immunofluorescence test. three of the groups reacted with virus antigens present on the membrane of the cells, whilst the fourth group failed to stain most live cells, suggesting specificity for an antigen expressed internally. representative monoclonals from the ...19853884736
respiratory syncytial virus. brief review. 19853885919
effect of in vitro inoculation of bovine respiratory syncytial virus on bovine pulmonary alveolar macrophage function.viruses may predispose the respiratory tract to the development of secondary bacterial pneumonia by impairing functions of alveolar macrophages. the effects of bovine respiratory syncytial virus (brsv) on selected functions of bovine pulmonary alveolar macrophages (pam) were examined in vitro. alveolar macrophages were obtained from nonsedated cattle, using a polypropylene tube passed intranasally into the lung. the pam lavaged from the lung were allowed to adhere to glass coverslips or plastic ...19853890630
management of bronchiolitis.the incidence, etiology, epidemiology, clinical presentation, treatment, prognosis, and prevention of bronchiolitis are discussed with a critical evaluation of the available studies on various therapeutic approaches. bronchiolitis is a lower respiratory-tract viral infection that affects 6-10% of all children below two years of age. respiratory syncytial virus (rsv) is the usual pathogen. the symptoms range from mild wheezing to severe respiratory distress. an infected child usually has a fever, ...19853891201
properties of a respiratory syncytial virus isolated from a sheep with rhinitis.a virus isolated from a yearling cross-bred ewe was identified as respiratory syncytial virus (rsv) by indirect immunofluorescence and by virus neutralization with bovine rsv antisera. the virus caused a mild conjunctivitis in 3-month-old lambs when inoculated alone. although clinical signs of pneumonia were not observed, there was gross and microscopic evidence of pulmonary inflammation in the lungs of lambs inoculated with either the sheep rsv isolate alone or in conjunction with pasteurella h ...19853893243
quantification of respiratory syncytial virus polypeptides in nasal secretions by monoclonal indirect enzyme-linked immunosorbent assay (elisa) which uses monoclonal antibody as solid-phase immunosorbent was developed to measure specific polypeptides of respiratory syncytial virus (rsv). the assay was used to examine 43 nasopharyngeal (np) aspirates from rsv-positive infants that had been examined previously for rsv by culture, direct immunofluorescence, and polyclonal antibody elisa. frozen np aspirates were serially diluted and examined for the 66k mol. wt. fusion glycoprotein (f), ...19853894575
practical recommendations for the detection of pediatric respiratory syncytial virus our private clinic-hospital setting, respiratory syncytial virus (rsv) was isolated from infants more frequently and sooner from nasal washes (84%; 4.2 days) than from throat swabs (45%; 5.5 days) or nasopharyngeal swabs (39%; 5.7 days). immunofluorescence of nasal wash cells identified 72% of the infants with virus isolations from nasal washes in less than one day. we therefore recommend the combination of isolation and immunofluorescence on nasal wash specimens for optimal detection of rsv- ...19853897268
development of cell-mediated cytotoxic activity in the respiratory tract after experimental infection with respiratory syncytial virus.the development of natural killer cell and other antibody-independent cellular cytotoxic response to rsv were studied in splenic and pulmonary mononuclear effector cells obtained from groups of 6-week-old cotton rats after subcutaneous (sc) or intranasal (in) immunization with live virulent or ultra-violet (uv) inactivated respiratory syncytial virus (rsv). no virus-induced cytotoxic activity was observed after sc immunization with live virus or in inoculation of inactivated non-infectious virus ...19853899431
the production of monoclonal antibodies against respiratory syncytial virus and its clinical applications.the availability of monoclonal antibodies against various viral components, either internal nucleoproteins or membrane proteins, has greatly advanced our understanding of the biochemistry of respiratory syncytial virus (rsv). the components responsible for serum neutralization have been identified by monoclonal antibodies, thus facilitating the development of synthetic rsv vaccine. a number of clinical tests have been devised using monoclonal antibodies for either qualitative or quantitative det ...19853899483
rapid detection of respiratory syncytial virus in nasopharyngeal secretions by enzyme-linked immunosorbent assay.a new enzyme-linked immunosorbent assay (elisa) respiratory syncytial virus antigen detection kit (ortho diagnostics, inc., raritan, n.j.) was compared with virus culture and with the indirect fluorescent antibody test (fat) by using fresh nasal washings from children with suspected respiratory syncytial virus infection. both uncentrifuged nasal washings and pellets from centrifuged split specimens were examined by elisa. the elisa was considered positive when the optical density was greater tha ...19853902879
ribavirin treatment of respiratory syncytial viral infection in infants with underlying cardiopulmonary disease.aerosolized ribavirin was evaluated in the treatment of respiratory syncytial virus lower respiratory tract disease in 53 infants, 36 of whom had underlying diseases. of the total infants, 26 were studied in a double-blind, placebo-controlled manner; 14 received ribavirin and 12 received placebo, a water aerosol, for an average of five days. when the infants with bronchopulmonary dysplasia and congenital heart disease treated with ribavirin were compared with those receiving placebo, the treated ...19853903239
immunoprophylaxis and immunotherapy of respiratory syncytial virus infection in the cotton rat.human convalescent antiserum to respiratory syncytial virus (rsv) administered intraperitoneally to cotton rats prior to rsv challenge provided near-complete protection from pulmonary infection. antiserum given subsequent to viral challenge reduced pulmonary viral titers 100-fold or greater within 24 h. sandoglobulin, a preparation of purified human igg with high titer of anti-rsv neutralizing activity, produced the same effects as convalescent antiserum. sandoglobulin was absorbed rapidly and p ...19853907188
calf pneumonia.infectious calf pneumonia is a high-mortality pneumonia of housed dairy-type calves. viruses are important etiologic agents and infection with bovine respiratory syncytial virus (rsv) and parainfluenza type 3 virus (pi-3 virus) may result in extensive, and sometimes fatal, lung damage. respiratory viral infections are frequently followed by mycoplasmal and secondary bacterial invasion of the lower respiratory tract, which increases the extent and severity of lung damage. bad housing, particularl ...19853907774
bovine respiratory syncytial virus.the current knowledge is reviewed in regards to the importance of bovine respiratory syncytial virus in the bovine respiratory disease complex. the epidemiology, clinical disease, pathologic findings, pathogenesis, diagnosis, treatment, and prevention of this viral disease are discussed.19853907775
respiratory syncytial virus infection in immunosuppressed animals: implications in human infection.neonatal cotton rats were treated with cyclophosphamide parenterally for three weeks before intranasal inoculation of live respiratory syncytial virus (rsv). immunosuppressive therapy resulted in severe depletion of lymphocytes from the peripheral circulation, the spleen, and the thymus. in contrast to normal rats, immunosuppressed animals developed severe pulmonary pathology with marked infiltration of foamy macrophages. persistent degeneration and regeneration of bronchial epithelial cells wer ...19853908616
[immunofluorescent demonstration of respiratory syncytial virus infection in calves].specific high-titre bovine conjugated antisera were obtained against respiratory syncytial virus. use was made of the reference strain nomi and the local isolate antonovo/448. the conjugates produced were shown to have equal qualities to those of the belgian conjugate used for comparison. the anti-antonovo/448 conjugated serum was used in the direct immunofluorescent method to demonstrate a respiratory syncytial virus antigen in organs of animals with acute respiratory diseases as well as to con ...19853909628
[acetone-celloidin method for the cytological and immunofluorescent study of cell cultures infected with various viruses].a method was worked out for the extraction of a cell monolayer from test tubes with the use of 10 per cent acetone solution of celloidin. this made it possible to carry out parallel cytologic and immunofluorescent studies of cultures infected with various viruses. in contrast to the alcohol-ether celloidin used in histology the use of the acetone solution of celloidin, suggested by us, preserved to a larger extent the viruses of different structure and composition, and, therefore, was shown to b ...19853909629
[structural aspect of the respiratory syncytial virus genome]. 19853914773
human mononuclear phagocytic cell interaction with some consideration of the peculiar relationship between paramyxoviridae and immunocompetent system, we studied interactions of measles virus, newcastle-disease virus and respiratory syncytial virus with human monocytes and macrophages differentiated "in vitro". mononuclear cells were challenged with three different paramyxovirus at different multiplicities of infection. infected cells were then checked for expression of specific viral antigens by immunofluorescent test (fa). moreover, we have eval ...19853916331
respiratory syncytial virus polypeptides. iv. the oligosaccharides of the glycoproteins.the cell-associated glycoproteins of respiratory syncytial (rs) virus included gp1 (90k), vp70 (70k), vgp48 (48k) and gp26 (26k). although present in infected cells, there was no vp70 in purified virus. trypsin treatment of infected cells removed 80 to 90% of vp70 as well as its products vgp48 and gp26. this suggested that most of the vp70 in the cell is located on the plasma membrane. the glycoproteins of purified rs virus (gp1, vgp48 and gp26) contain mannose, galactose and fucose as well as g ...19853919149
correlation of a disease scoring system with arterial po2 values in respiratory syncytial virus infection in calves.the mean arterial po2 value measured in blood obtained by puncture of the brachial artery of 20 calves with acute clinical signs of a bovine respiratory syncytial virus (brsv) infection was 8.4 +/- 1.9 kpa. the values differed significantly from arterial po2 values of eleven healthy calves (mean 14.2 +/- 1.5 kpa). a disease scoring system is presented based on the type of respiration and the findings on auscultation. a high correlation (r = -0.87) was found between disease scores and arterial po ...19853925615
respiratory syncytial virus outbreak at an institution for the mentally retarded--washington. 19853932832
serological and virological investigations of young children with acute respiratory syndrome associated with respiratory syncytial virus.from january 1979 to march 1979, 341 young children from the metropolitan area of naples, italy, were hospitalized with respiratory virus disease. diagnosis of patients made from virus isolation and seroconversion indicate that the respiratory syncytial virus was a primary cause of this acute respiratory syndrome.19853967475
antibody response to respiratory syncytial virus structural proteins in children with acute respiratory syncytial virus infection.the purified respiratory syncytial virus (rsv), randall strain contained 10 polypeptides (72,000 molecular weight [72k], 66k, 48k, 42k, 40k, 36k, 30k, 23k, 18k, and 15k), 8 of which proved to be virus specific, and polypeptides 48k and 23k were glycosylated. in addition, a high-molecular-weight (150k), virus-specific glycopolypeptide was immunoprecipitated from rsv-infected cell lysate. the antibody response in human sera serially collected from children with primary rsv infection was mainly dir ...19853973971
monoclonal capture antibody elisa for respiratory syncytial virus: detection of individual viral antigens and determination of monoclonal antibody enzyme-linked immunosorbent assay (elisa) for respiratory syncytial virus (rsv) that employs solid-phase monoclonal antibodies was developed. rsv antigens bound by these monoclonal capture antibodies were detected by addition of a polyclonal bovine antiserum, followed anti-bovine enzyme conjugate and enzyme substrate. the sensitivity and specific of the assay were determined by titrations of the solid-phase antibodies and by antigen titrations with both unpurified rsv-infected cell culture ma ...19853981003
[clinical aspects of acute and chronic respiratory syncytial virus infection in adult polyclinic patients].a total of 224 workers and employees engaged at the smolensk flax spinning plant, suffering from acute respiratory diseases, were examined on an outpatient basis. virus etiology of the diseases was established in 84 patients (37.7%) with the aid of the serological tests (hi and cf) and immunofluorescent techniques. respiratory syncytial virus infection was diagnosed in 19 patients (8.5%). as regards the clinical picture the symptoms of intoxication were unmarked. half the patients demonstrated m ...19853983830
[experimental infection of lambs with strains of the bovine respiratory syncytial virus].lambs were successfully infected with bovine strains of a respiratory syncytial virus. when the nomi reference strain and the local antonovo strain of the rsv were used i/v and via the trachea the animals reacted on the 3rd to 5th day with dizziness, dyspnea, conjunctivitis, nasal discharges, etc., more strongly manifested with the use of the antonovo strain. the temperature reaction started on the 3rd day and receded on the 7th day, with a second peak on the 8h day. serologically, rsv-antibodie ...19853992914
characterization of the 10 proteins of human respiratory syncytial virus: identification of a fourth envelope-associated protein.a total of 13 respiratory syncytial (rs) virus specific polypeptides were identified by pulse-chase metabolic labeling of infected hep-2 cells. ten of the 13 proteins were shown to be unique. they were the l, g, f (f1, f2), n, p, m, 24k, 14k, 11k and 9.5k proteins. these conclusions were based on peptide mapping and on previous work showing that each of 10 polypeptides are coded for by a unique mrna. the seven largest proteins, l, g, f (f1, f2), n, p, m and 24k were identified clearly as virion ...19853993233
experimental bovine respiratory syncytial virus infection in conventional calves: light microscopic lesions, microbiology, and studies on lavaged lung cells.conventionally raised male holstein calves, 1 month of age, were infected by intranasal and intratracheal inoculation with bovine respiratory syncytial virus. viral antigen was identified by fluorescence microscopy most commonly in the cytoplasm of tracheal and bronchial epithelial cells 3 to 5 days after inoculation. cytoplasmic viral antigen was identified also in nasal, nasopharyngeal, bronchiolar, and alveolar epithelial cells and in alveolar macrophages. bronchitis and tracheitis, character ...19853994121
experimental bovine respiratory syncytial virus infection in conventional calves: ultrastructural respiratory lesions.the morphogenesis and repair of airway and alveolar injury induced by bovine respiratory syncytial virus (brsv) was studied ultrastructurally in conventional calves to characterize pulmonary cell types susceptible to viral infection and cytopathologic changes associated with infection. viral nucleocapsids and budding virions were present in tracheal and bronchial ciliated and nonciliated epithelial cells and mucous cells 3, 5, and 7 days after inoculation and in bronchiolar ciliated and noncilia ...19853994122
lobar collapse with respiratory syncytial virus a study of 30 children with uncomplicated respiratory syncytial virus (rsv) pneumonias, a high incidence of lobar collapse (8/30-26%) was noted. this involved the right upper lobe in seven patients and the left upper lobe in one patient. it is probably attributable to anatomical predispositions, sloughing of necrotic epithelium, and stimulation of mucus production. lobar collapse should be considered part of the spectrum of rsv pneumonitis.19854000751
respiratory syncytial virus-specific ige responses following infection: evidence for a predominantly mucosal order to determine whether ige production occurs predominantly at mucosal or systemic sites, we studied the production of respiratory syncytial virus (rsv)-specific antibody in serum and nasopharyngeal secretions (nps) from 41 patients with rsv infection using an enzyme-linked immunosorbent assay. rsv-ige was found in higher titer in samples of nps than in simultaneously obtained serum specimens at all phases of illness. despite the excess dilution incurred in the collection process, rsv-ige ...19854000767
severe respiratory syncytial virus infection: clinical features, nosocomial acquisition and outcome.respiratory syncytial virus (rsv) frequently causes bronchiolitis in young infants. rsv infection was established by culture, serologic response, immunofluorescence of secretions or lung tissue or typical histology on pathologic examination of the lungs in 10 patients who were hospitalized at children's medical center, dallas. eight were infants 4 days to 3 months old, one was 9 months and one was 16 months old. seven patients were afebrile but had rapidly progressive diffuse infiltrates on ches ...19854000987
respiratory syncytial virus polypeptides. v. the kinetics of glycoprotein synthesis.the cell-associated glycoproteins of respiratory syncytial virus included gp1 (90k), vp70 (70k), vgp48 (48k) and gp26 (26k). the time course of virus protein synthesis in hela cells revealed that the nucleocapsid protein (vpn41) was the first to appear at 11 h post-infection followed by the appearance of the other viral proteins at 16 h post-infection. pulse-chase experiments with [3h]leucine or [35s]methionine demonstrated that the precursor for vgp48 and gp26 was an uncleaved protein of 70k (v ...19854009147
mrna sequence of three respiratory syncytial virus genes encoding two nonstructural proteins and a 22k structural mrna sequence of two human respiratory syncytial viral nonstructural protein genes and of a gene for a 22,000-molecular-weight (22k) protein was obtained by cdna cloning and dna sequencing. sequences corresponding to the 5' ends of the respective transcripts were deduced directly by primer extension and dideoxy nucleotide sequencing of the mrnas. the availability of a bicistronic clone (prsc6) confirmed the gene order for this portion of the genome. contrary to other unsegmented negative-stra ...19854009789
comparison of a newly developed enzyme-linked immunosorbent assay with complement fixation and neutralisation tests for serology of bovine respiratory syncytial virus indirect double antibody sandwich enzyme-linked immunosorbent assay (elisa) was developed for the detection and titration of serum antibodies to bovine respiratory syncytial virus (brsv). the elisa was compared with a complement fixation (cf) test and a test for virus neutralising antibody in serum (virus neutralisation [vn] test). testing sera collected in dairy herds revealed the closest correlation between the results of the elisa and the cf test with respect to brsv antibody titres. the v ...19854012035
an outbreak of respiratory syncytial virus infection in a continuing-care geriatric ward.a small outbreak of respiratory syncytial virus infection in a continuing-care geriatric ward during the period december 1982 to march 1983 is reported. it demonstrates that respiratory syncytial virus infection can cause serious lower respiratory tract infection even in patients without any previous history of chest disease.19854013906
respiratory tract infections. goals for 1995.goals to be identified for 1995, a decade hence, in the prevention, diagnosis, and management of respiratory tract infections may conveniently be divided into diagnostic goals and goals in therapy and prophylaxis. major diagnostic goals for bacterial, viral, and mycoplasmal infections of the respiratory tract focus on the development of systems to identify microbial components, such as specific antigens or segments of dna, using monoclonal antibody techniques or dna probes for hybridization. spu ...19854014289
serologic studies of bovine respiratory syncytial virus in minnesota cattle.serum antibody titers to bovine respiratory syncytial virus were determined for 559 cattle. serum samples were obtained through the minnesota state-federal brucellosis laboratory and were collected over a 1-year period. results of this study revealed an antibody prevalence of 65.5% to bovine respiratory syncytial virus. the distribution of antibody titers is presented, as well as analysis of titers based on breed, sex, and age of the cattle.19854014838
etiology of community-acquired pneumonia in patients requiring hospitalization.the etiology of community-acquired pneumonia was studied in 127 patients with roentgenologically verified pneumonia who needed hospitalization. etiology was determined on the basis of a positive blood culture and/or a significant antibody titer increase. streptococcus pneumoniae was the probable etiological agent in 69 patients, nontypeable haemophilus influenzae in five patients, streptococcus pyogenes in two patients, and legionella pneumophila and staphylococcus aureus in one patient each. ev ...19854018066
quantitative aspects of passive immunity to respiratory syncytial virus infection in infant cotton rats.the amount of passively acquired serum respiratory syncytial virus (rsv)-neutralizing antibodies required to protect the respiratory tract of cotton rats against infection was studied. infant cotton rats were inoculated intraperitoneally with various dilutions of a single pool of sera derived from cotton rats convalescent from rsv infection. after 24 h, these animals were inoculated with rsv intranasally. virus replication in the respiratory tract was suppressed in cotton rats which had a serum ...19854020957
[incidence of respiratory syncytial virus infection in case records of a pediatric general medicine department]. 19854021934
kinetics of synthesis of respiratory syncytial virus glycoproteins.the synthesis of the two respiratory syncytial (rs) virus glycoproteins (vp66 and vp84) was examined under standard conditions and after treatment with tunicamycin and monensin. the protein backbone for vp66, the fusion protein (f1,2) is cotranslationally glycosylated to form f0, which is cleaved to form f1,2 by 20 min of chase. monensin treatment inhibited the cleavage of f0 over an 80 min chase period, indicating that this occurred late in the transit of f0 through the golgi apparatus or after ...19854031826
the childhood health effects of an improved water supply system on a remote panamanian island.the incidence of diarrhea, respiratory disease, and skin infections was prospectively determined after the introduction of a system which distributed unlimited quantities of high quality fresh water to each of the 150 housing units on tupile, an island devoid of fresh water located off panama's caribbean coast and inhabited by 1,500 cuna indians. tupile residents used 7.1 liters of water/person/day compared to the 2.3 usage rate of inhabitants on achutupo, the control island. despite ready avail ...19854037183
outbreak of respiratory syncytial virus in france.a report is given of an outbreak of respiratory syncytial virus infection in a neonatal unit in france. twenty-three of 32 infants were infected (72%) despite infection control procedures. prophylactic administration of non-specific gamma globulins was associated with a significant decrease in infection rate (p less than 0.05). the administration of transfer factor to infected infants was also associated with a significantly lower rate of severe respiratory diseases (p less than 0.05).19854043060
studies of passive immunotherapy for infections of respiratory syncytial virus in the respiratory tract of a primate model. 19854045247
cellular reactivity to respiratory syncytial virus in human colostrum and breast milk.colostrum and breast-milk samples were taken from 23 mothers between 2 days and 7 weeks postpartum and were examined for the presence of cellular reactivity to respiratory syncytial (rs) virus using a lymphocyte transformation assay. positive responses were detected in nine of the 23 (39%) samples taken at 2-5 days postpartum, but this reactivity was undetectable at 3 weeks. positive responses developed in a further three mothers during the 3-7-week period of lactation, suggesting a response to ...19854045438
severe respiratory disease in dairy cattle in new york state associated with bovine respiratory syncytial virus infection.severe respiratory disease associated with bovine respiratory syncytial virus (brsv) infection has been identified in dairy cattle in new york state. the cases identified occurred in dairy calves and heifers. the disease was characterized in 4 animals by pathologic changes including interstitial pneumonia, necrotizing bronchiolitis with multinucleated syncytial epithelial cells and interstitial emphysema. brsv antigen was demonstrated in lung samples or was isolated in tissue culture in all 4 ca ...19854053607
an epizootic of respiratory syncytial virus infection in a dairy herd.respiratory syncytial virus was the cause of a severe epizootic of bovine respiratory tract disease. the virus, isolated from a sick cow during the epizootic, produced cytopathic effect in a bovine turbinate cell line 14 days after it was inoculated. additional support for the diagnosis came from the results of pathologic and serologic examinations. lesions consistently present were severe necrotizing bronchiolitis and epithelial syncytia projecting from bronchiolar and alveolar walls. also, in ...19854055489
severe rsv bronchiolitis in an immunocompromised child.a 9-year-old with immunodeficiency developed a severe, diffuse respiratory illness that necessitated mechanical ventilation. open lung biopsy revealed respiratory syncytial virus (rsv) as the sole pathogen. rsv detection should be included in the differential diagnosis of diffuse lung disease in an immunocompromised child.19854064461
microneutralization test for respiratory syncytial virus based on an enzyme immunoassay.virus infectivity and antibody neutralization titers for respiratory syncytial virus were determined in cell cultures in microtiter plates. after an appropriate incubation period, the cells were fixed, and an enzyme-linked immunosorbent assay was performed directly in the microtiter plates for detection of virus. results could be read and recorded automatically, which is especially helpful when running large numbers of tests.19854066916
comparison of ortho respiratory syncytial virus enzyme-linked immunosorbent assay and hep-2 cell culture.two hundred seventy nasopharyngeal aspirates were tested in duplicate with the ortho diagnostics, inc. (raritan, n.j.), respiratory syncytial virus antigen enzyme-linked immunosorbent assay. the test was sensitive (80 to 82%) and specific (96%) when compared with cell culture. the enzyme-linked immunosorbent assay detected seven antigen-positive specimens not among the 71 specimens that were positive for respiratory syncytial virus in cell culture. a blocking test confirmed those specimens as tr ...19854066920
bronchodilator response during acute viral bronchiolitis in infancy.bronchodilator responsiveness was assessed by measuring specific respiratory conductance before and after inhalation of aerosolized bronchodilator in 50 infants who had acute bronchiolitis due to respiratory syncytial virus infection. thirty per cent of the infants showed an improvement in specific conductance. responders could not be differentiated from nonresponders by family histories of atopy, eosinophil counts, or immunoglobulin levels in blood and nasal secretions. eighty-three per cent of ...19854069797
respiratory syncytial virus envelope glycoprotein (g) has a novel structure.amino acid sequence of human respiratory syncytial virus envelope glycoprotein (g) was deduced from the dna sequence of a recombinant plasmid and confirmed by limited amino acid microsequencing of purified 90k g protein. the calculated molecular mass of the protein encoded by the only long open reading frame of 298 amino acids was 32,588 daltons and was somewhat smaller than the 36k polypeptide translated in vitro from mrna selected by this plasmid. inspection of the sequence revealed a single h ...19854069997
[bovine respiratory syncytial virus as a pathogen in the respiratory tract diseases of cattle: a clinical and seroepidemiologic study in switzerland]. 19854071019
[bovine respiratory syncytial virus (brsv) in switzerland: a serologic study]. 19854071020
[infections with respiratory syncytial virus in infants and young children]. 19854080005
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