TitleAbstractYear(sorted ascending)
seven complementation groups of respiratory syncytial virus temperature-sensitive mutants.fifteen temperature-sensitive mutants of the rsn-2 strain of respiratory syncytial virus have been classified into six complementation groups, two of which appeared to be homologous with two of the three complementation groups of the a2 strain described by wright et al. (p. f. wright, m. a. gharpure, d. s. hodes, and r. m. chanock, arch. gesamte virusforsch, 41:238--247). thus seven complementation groups of respiratory syncytial virus, designated a, b, c, d, e, f, and g, have been defined. the ...1978702637
initiation and maintenance of persistent infection by respiratory syncytial virus.propagation of cells infected with temperature-sensitive (ts) mutants of respiratory syncytial (rs) virus at nonpermissive temperature (39 degrees c) resulted in cytolytic, abortive, or persistent infection, depending on the mutant used to initiate infection. five mutants from complementation group b produced cytolytic or abortive infections, whereas a single mutant (ts1) from group d and a noncomplbmenting mutant produced persistent infections. the persistently infected culture initiated by mut ...1978702647
[study and identification of the respiratory syncytial virus isolated from a leukemic cow].a virus was isolated from the lymph nodes of a cow. by its cultural, physical, chemical, and biologic properties it proved identical with the standard bovine respiratory syncytial virus. it multiplied well in cell cultures of calf kidney at ph 7--7.5. introduced in 20-day-old guinea pigs the virus caused anorexia, bristling up of hairs, stunting, and occasionally death. the inoculated guinea pigs developed pneumonia of a proliferative type with langhans' giant cells. the interrlationship between ...1978706146
behavior of respiratory syncytial virus in piglet tracheal organ culture.piglet tracheal organ cultures were infected with respiratory syncytial virus (rsv) and observed for 21 days. light and immunofluorescence microscopy demonstrated destruction of the ciliated epithelial cells and the presence of viral antigens in the epithelium. virus was shed in high titer for 12--19 days. ciliostasis could be quantitated, and it was shown that several strains of rsv grew and damaged tracheal organ cultures in a similar fashion. a temperature-sensitive mutant of rsv, ts-1, was e ...1978712118
breast-feeding as protection against respiratory syncytial virus. 1978719443
neutralisation of respiratory syncytial virus by cat serum. 1978723929
control of nosocomial respiratory syncytial viral infections.we evaluated methods to control the spread of respiratory syncytial virus (rsv) on our infants' ward during a community outbreak of rsv infection. methods included isolation and cohorting of infected infants, strict handwashing, use of gowns, and the cohorting of staff to the ill infants. of 123 infants studied, 36 were admitted with rsv infections. of the remaining 87 contact infants, eight (19%) acquired nosocomial rsv disease. three of the eight developed pneumonia and one died. of the 43 sta ...1978724317
radiologic appearance of viral disease of the lower respiratory tract in infants and children.the radiologic findings in 123 hospitalized children with viral disease of the respiratory tract due to adenovirus, respiratory syncytial virus, parainfluenza, influenza, measles, or herpes virus were retrospectively reviewed. bronchial wall thickening, peribronchial shadowing and/or associated perihilar streaking were present in 107 cases. patchy pulmonary shadowing thought to reflect disease at a sublobular or lobular level was present in 72 cases, while areas of coalescent more homogeneous pu ...1978202157
[antiviral antibodies in acute laryngotracheobronchitis].antibodies to influenza a2, influenza b, parainfluenza 2 and 3, adenovirus and respiratory syncytial viruses were determined in thirty nine children with acute laryngotracheobronchitis; nine cases were not included in the analysis because of the presence of anticomplementary sera; 40% of cases were positive, 20% to influenza a2, 10% to influenza b (one case of double infection with influenza b and parainfluenza 2 viruses), 6.7% to respiratory syncytial virus and 3.3% to parainfluenza 2. results ...1978202293
detection of antibody to bovine syncytial virus and respiratory syncytial virus in bovine fetal serum.batches of commercial fetal bovine serum, described by the suppliers as antibody-free, all contained antibody to bovine syncytial virus (bsv) when tested by indirect immunofluorescence. antibody to bovine respiratory syncytial virus (rsv) was not detected in these sera. twenty-four percent of individual fetal bovine sera contained antibody to bsv, and 14% contained antibody to rsv when tested by indirect immunofluorescence. bsv antibody titers in fetal sera from dams with high bsv antibody level ...1978100508
evaluation of five temperature-sensitive mutants of respiratory syncytial virus in primates: ii. genetic analysis of virus recovered during infection.five temperature-sensitive (ts) mutants of respiratory syncytial (rs) virus (ts-1, ts-1 ng-1, ts-1 ng-16, ts-2, and ts-7), previously evaluated forinfectivity and virulence in chimpanzees and owl monkeys, were also assayed for in vivo genetic stability. none of the five mutants tested was completely stable genetically. thus, virus which had lost some or all of the ts property was recovered from each infected chimpanzee. significantly, each ts-1 ng-1 isolate retained some degree of temperature se ...1978104003
evaluation of five temperature-sensitive mutants of respiratory syncytial virus in primates: i. viral shedding, immunologic response, and associated illness. 1978104004
[etiologic importance of the respiratory syncytial virus in bronchiolitis]. 1978117525
respiratory syncytial virus. i. concentration and purification of the infectious virus.respiratory syncytial (rs) virus can be purified without losing its infectivity provided that each step of purification is carried out using nt buffer containing over 20% sucrose. firstly, the virus grown on hes cells is efficiently removed from the culture fluid by precipitating with polyethylene glycol (peg) 6,000, and the precipitate is suspended in a small amount of 20% sucrose-nt buffer, which results in about a 24-fold concentration of the original material. then this suspension is centrif ...1978153087
experimental respiratory syncytial virus pneumonia in cebus monkeys.into 14 juvenile cebus monkeys that lacked serum antibodies for rs virus 10(8) plaque-forming units (pfu) of wild-type respiratory syncytial (rs) virus were inoculated transtracheally. roentgenographic evidence of pneumonia developed in 13 of 14 infected animals. gross pathologic changes occurred in each of the 13 monkeys that were sacrificed. patchy areas of red consolidation were seen in the lower lobes 24 hours after inoculation, and there was progression to gray consolidation seven days late ...1978210254
pneumoviruses: the cell surface of lytically and persistently infected cells.human embryonic lung (mrc-5), feline embryo (fea), mink lung (mv1lu) and monkey kidney (bsc-1) cells infected by respiratory syncytial virus showed characteristic morphological changes when viewed by scanning electron microscopy. the surfaces of respiratory syncytial virus-infected cells developed a profusion of slender filaments after 48 h incubation at 31 degrees c. similar changes in surface morphology were observed in bsc-1 cells infected by murine pneumonia virus. filament production theref ...1979118236
respiratory syncytial virus infection in owl monkeys: viral shedding, immunological response, and associated illness caused by wild-type virus and two temperature-sensitive mutants.intranasal inoculation of owl monkeys with wild-type respiratory syncytial virus induced upper respiratory tract disease in each of seven animals. the response of owl monkeys to two highly defective, temperature-sensitive, multiple-lesion mutants was then compared to the pattern seen with wild-type respiratory syncytial virus. these mutants, ts-1 ng-1 and ts-1 ng-16, were derived from the ts-1 mutant that had been remutagenized with nitrosoguanidine (ng). previously the ts-1 ng-1 and ts-1 ng-16 ...1979118925
croup and pneumonia in human infants associated with a new strain of respiratory syncytial virus. 1979119004
the longitudinal approach to the pathogenesis of respiratory disease.longitudinal observations were made of a well-defined population of children at a day care center in an investigation of the pathogenesis of infections due to respiratory syncytial virus (rsv) and mycoplasma pneumoniae. a single rsv infection induced a modest but significant degree of resistance to further rsv infection in these children. age and immunity seemed to interact to decrease the intensity of the clinical expression of illness associated with rsv infection. infants and young children h ...1979121783
further investigations of live respiratory syncytial virus vaccine administered parenterally. 1979419156
reye's syndrome associated with respiratory syncytial virus upper respiratory tract infection in a 22-month-old boy was followed by rapid loss of consciousness, hypoglycaemia, uraemia, and death. necropsy examination showed fatty change of liver and kidneys, severe cerebral oedema, bronchiolitis, and endocardial fibroelastosis affecting the left ventricle. immunofluorescence staining showed infection with respiratory syncytial virus (rsv). the clinical and pathological findings were those of reye's syndrome, not previously reported accompanying rsv in ...1979420528
cell-free and cell-bound antibody in nasal secretions from infants with respiratory syncytial virus infection.twenty-two infants under 9 months of age hospitalized with bronchiolitis or pneumonia due to respiratory syncytial virus (rsv) were serially sampled to determine the pattern of secretory antibody response. using double labeling techniques, we found several types of immunoglobulin in secretions: cell-free antibody to rsv of the immunoglobulin a (iga), immunoglobulin g (igg), and immunoglobulin m (igm) classes; and immunoglobulins of all three classes bound to rsv-infected cells shed from the nasa ...1979422241
cell-mediated immune response to respiratory syncytial virus infection: relationship to the development of reactive airway disease.the possibility that cell-mediated immunity might play a role in the pathogenesis of infection with respiratory syncytial virus was evaluated in a study of 39 infants. infection with rsv was confirmed by identification of virus in nasopharyngeal secretions using immunofluorescence, and by tissue culture infectivity. cmi, as determined by a whole blood lymphocyte transformation technique, was evaluated in samples taken 0 to 10 and 20 to 60 days after the onset of illness. patients diagnosed as ha ...1979423016
the polypeptides of human respiratory syncytial virus: products of cell-free protein synthesis and post-translational modifications. 1979425320
respiratory syncytial virus and sudden infant death. 1979440395
respitory syncytial virus and sudden infant death. 1979440396
respiratory syncytial virus and sudden infant death. 1979440397
respiratory syncytial virus polypeptides: their location in the virion. 1979442538
measles and rubella virus antibodies in patients with multiple sclerosis. a longitudinal study of serum and csf specimens by radioimmunoassay.a longitudinal study on rubella, measles, and respiratory syncytial virus antibodies in serial serum and csf specimens from 20 multiple sclerosis (ms) patients was performed, using solid-phase radioimmunoassay. albumin and immunoglobulin g (igg) levels were also measured to check the integrity of the blood-brain barrier and the intrathecal igg production. all the patients had local igg production in their cna. a local antibody production against one or more of the viruses studied was evident in ...1979444095
immunoprophylaxis of respiratory syncytial virus infection in the infant ferret.infant ferrets can be protected from respiratory syncytical virus challenge at 3 days of age by gestational infection of their mothers. ferrets acquire their immunity to respiratory syncytial virus postpartum via immunizing products of lactation. the level of protection against viral replication correlates with the maternal serum neutralizing titer or a concomitant factor. passive administration of adult ferret serum with a neutralizing titer of 1:1024 or greater, either i.p. or orally does not ...1979448139
ontogeny of the ferret humoral immune response.infant ferrets are born with nearly undetectable immunoglobulin levels, but by 9 days of age the infant ferret serum contains 77, 29, and 13% of adult mean serum levels of igg, iga, and igm. transmucosal uptake of igg by the infant ferret occurred for the first 30 days of life. the specific anti-respiratory syncytial virus neutralizing titer of whole milk was 5.5 times higher than maternal serum despite a lower concentration of immunoglobulins in the milk.1979448158
the etiologic and epidemiologic spectrum of bronchiolitis in pediatric develop a broad understanding of the causes and patterns of occurrence of wheezing associated respiratory infections, we analyzed data from an 11-year study of acute lower respiratory illness in a pediatric practice. although half of the wari occurred in children less than 2 years of age, wheezing continued to be observed in 19% of children greater than 9 years of age who had lower respiratory illness. males experienced lri 1.25 times more often than did females; the relative risk of males fo ...1979448557
characterization and identification of a bovine respiratory syncytial virus isolated from young calves.two identical viruses designated 371 and 375 were recovered from nasal secretions of 2 of 7 calves in a beef cow-calf herd in which calves (45 to 105 days of age) had signs of acute respiratory tract disease. the cytopathic, morphologic and physico-chemical characteristics of the isolates were those of bovine respiratory syncytial virus. although a humoral antibody response to bovine respiratory syncytial virus was not observed, it was concluded that this virus probably had a part in the respira ...1979453673
clinical and physiological manifestations of bronchiolitis and pneumonia. outcome of respiratory syncytial virus.the physiological abnormalities and clinical correlates of 32 infants consecutively hospitalized with lower respiratory tract disease from respiratory syncytial virus (rsv) were studied in an attempt to characterized the infant most at risk for the acute and long-term complications of rsv infection. arterial oxygen saturation (sao2) determinations were obtained daily by means of an ear oximeter. on admission all infants were hypoxemic with a mean sao2 of 87% (range, 74% to 95%). the mean of the ...1979463835
demonstration of anti-rubella antibody-secreting cells in rheumatoid arthritis the present study we describe a plaque-forming cell assay using erythrocytes coated with viral antigen, which detected anti-viral antibody-secreting cells against various viral antigens. these anti-viral antibody-secreting cell were studied in normal individuals with known viral infections and in rheumatoid arthritis patients. rubella anti-viral antibody-secreting cells were present after induction in the peripheral blood of eight out of ten patients. no plaques were seen before induction. sy ...1979472665
presumed respiratory syncytial virus pneumonia in an adolescent compromised host.a 15-year-old boy with nephrotic syndrome, renal insufficiency, and cutaneous anergy had severe pneumonia with pleural effusion. there was serologic evidence of respiratory syncytial virus infection, and extensive microbiologic, histologic, and serologic studies failed to identify any other etiologic agent. respiratory syncytial virus as a possible cause of severe pneumonia in immunocompromised hosts has not been previously reported.1979472848
lymphocyte transformation in response to antigens of respiratory syncytial virus. 1979482291
respiratory syncytial virus: a community problem. 1979486998
[results obtained using an attenuated bovine syncytial virus vaccine against bronchopneumonia of yearlings (author's transl)].bronchopneumonia of yearlings is a respiratory infection with bovine respiratory syncytial virus (brs virus) occurring as a herd disease in young cattle. in severe cases, the clinical picture is identifiable; characteristic features are laboured respiration and a high morbidity rate. following an acute onset, atypical interstitial pneumonia is observed on postmorten examination and bacteriological studies are negative. an attenuated live brs virus vaccine was used against bronchopneumonia of yea ...1979494211
solid-phase radioimmunoassay of serum immunoglobulin a antibodies to respiratory syncytial virus and adenovirus.a solid-phase radioimmunoassay for detecting respiratory syncytial virus and adenovirus serum immunoglobulin a (iga) antibodies was developed. an antigen consisting of purified adenovirus type 2 hexons or a crude lysate of respiratory syncytial virus-infected cells was first adsorbed onto polystyrene beads. the coated beads were then incubated with dilutions of serum, and iga antibodies which attached to the solid-phase virus antigen were subsequently detected with 125i-labeled anti-human alpha ...1979511988
[the significance of respiratory syncytial virus as pathogen in respiratory tract infections in infants (author's transl)].ten out of twenty babies aged up to twelve months and admitted during the months february and march 1978 were shown to be infected with respiratory syncytial virus. during the first 6 months of life the disease usually presents as bronchiolitis. serologically only neutralising antibodies were demonstrable in this age group. iga was usually raised in babies with bronchopneumonia. an allergic reaction between virus antigen and maternal antibodies is considered to occur, assuming an activation of t ...1979516739
a respiratory syncytial virus outbreak in a transitional care nursery. 1979517481
experimentally induced respiratory syncytial viral infection in lambs.signs of clinical illness, lesions, and seroconversion were observed in lambs experimentally inoculated with a respiratory syncytial virus of bovine origin. signs of clinical illness were mild and consisted of fever and hyperpnea. multifocal areas of interstitial pneumonia were seen in lambs necropsied during the period of clinical response. all lambs seroconverted to viral antigen, and the virus was isolated from two of the lambs.1979517825
respiratory syncytial virus with apnoea treated with theophylline. 1979522760
lesions in lambs experimentally infected with bovine respiratory syncytial virus.respiratory syncytial virus was inoculated intratracheally into five 1-week-old lambs. three of the lambs responded clinically with fever, hyperpnea, and listlessness. pulmonary lesions consisted of multifocal areas of consolidation, with necrosis of individual epithelial cells of the airways and accumulation of necrotic debris, macrophages, and few neutrophils in terminal airways and alveoli. pulmonary septa in affected areas were infiltrated with numerous macrophages and lymphocytes. viral par ...1979525867
experimental respiratory syncytial virus infection in feeder-age lambs.signs of disease, lesions, and serologic response were seen in six 6-month-old-lambs experimentally inoculated with respiratory syncytial virus (rsv). a transient febrile response, mild hyperpnea, and listlessness were seen. multifocal areas of interstitial pneumonia and bronchiolitis were seen in lambs necropsied during the period of clinical response. a serologic response to rsv was seen in all lambs, and rsv was recovered from five lambs.1979525890
effect of 2-deoxy-d-glucose and glucosamine on bovine respiratory syncytial virus.2-deoxy-d-glucose (2-dg) and glucosamine reversibly inhibited the replication and cytopathic effects of bovine respiratory syncytial virus in bovine turbinate cell cultures. the inhibitors were effective when added up to 12 hours after cell cultures were inoculated. their effectiveness decreased as the time between inoculation of cells and drug treatment lengthened. the sugars did not inactivate the virus directly, and inoculation of drug-treated cells in drug-free medium produced normal yield o ...1979525934
respiratory syncytial virus, 1979: scotland. 1979526842
secretory immunity in influenza.the dynamics of secretory antibody formation, the duration of secretory antibody preservation, and changes in the concentration of secretory antibodies to antigens other than influenza virus were studied in 64 patients with influenza a, 105 patients with influenza b, and 23 persons who had had influenza a. severe forms of influenza a were accompanied by antibody accumulation in sera and nasal secretions; in some cases of mild forms of this infection, this process was limited by the humoral immun ...1979541522
respiratory syncytial virus infection in inbred mice.respiratory syncytial virus infected the nose and lungs of each of 20 strains of inbred mice, with viral titers varying 100-fold from least permissive to most permissive strains. viral titers appeared to be under genetic control, but did not correlate with the h-2 haplotype.1979546793
[respiratory infection epidemic during the winter of 1979 in umbria].the aa, report the results of virological and serological investigations performed during an epidemic of acute respiratory diseases in umbria. the epidemic was associated with respiratory syncytial virus; other virus infections were sporadic.1979553517
neonatal respiratory syncytial virus infection.respiratory syncytial virus infections are thought to be uncommon in the first month of life. during a community outbreak, we prospectively studied such infection in our neonatal units. of 82 neonates studied, 66 were hospitalized for six days or longer, and 23 (35 per cent) acquired this virus. four infants died, two unexpectedly. infected infants had a significantly shorter gestation and birth weight. illness was often atypical, with nonspecific signs, especially in infants under three weeks o ...1979759915
respiratory syncytial virus in nurseries. 1979759920
respiratory-syncytial-virus infections, reinfections and immunity. a prospective, longitudinal study in young better understand acquired immunity to respiratory-syncytial-virus infections, we analyzed data from a 10-year study of respiratory illness in normal children who were followed longitudinally from early infancy. immunity was measured in terms of failure to become infected or reduction in severity of clinical illness upon reinfection. outbreaks of infections occurred seven times over the 10-year-period. during epidemics the attack rate for first infection was 98 per cent. the rate for second i ...1979763253
observations on outbreaks of respiratory disease in calves associated with parainfluenza type 3 virus and respiratory syncytial virus four outbreaks of indoor calf pneumonia, dyspnoea was a prominent clinical finding. at necropsy it was associated with pneumonia involving the cranial lobes of the lung and severe pulmonary emphysema. histological examination of lung tissue revealed bronchiolitis and alveolitis with alveolar epithelial cell hyperplasia and multinucleate syncytium formation. intraalveolar haemorrhage, intra-alveolar oedema and hyaline membrane formation were also noted. in all cases parainfluenza type 3 (pi3) ...1979219584
clinical significance of pulmonary function tests. alterations in pulmonary function following respiratory viral infection.respiratory viral illness is a major cause of morbidity in both adults and children. this report focuses on both the acute and chronic effects on respiratory function of these ubiquitous infections. infant airways are particularly vulnerable due to the relatively low conductance in immature peripheral airways. bronchiolitis, caused predominantly by respiratory syncytial virus, is the most important of these viral illnesses and is emerging as a major risk factor for the subsequent development of ...1979225132
respiratory syncytial virus in naples outbreak. 1979255512
respiratory syncytial virus theory on naples' deaths. 1979257184
neonatal respiratory-syncytial-virus infection. 1979313006
[bronchopneumonia of yearlings (pinkengriep). bovine respiratory syncytial virus as a cause of atypical interstitial pneumonia in young cattle (author's transl)]. 1979315112
antibody-dependent cell-mediated cytotoxicity against cells infected with respiratory syncytial virus: characterization of in vitro and in vivo in vitro 51cr release assay for human antibody-dependent cell-mediated cytotoxicity (adcc) against hela cells infected with respiratory syncytial virus (rsv) has been characterized by using leukophoresed and adherent cell-depleted adult lymphocytes. lymphoytes from rsv seronegative children were also competent as effector cells. sera from children with :1) primary and recurrent natural rsv infections, or 2) live attenuated rsv vaccine infection were examined to characterize the behavior of ad ...1979571890
intramuscular inoculation of live respiratory syncytial virus induces immunity in cotton rats.intramuscular inoculation of infant or weanling cotton rats with 10(2.2) to 10(4) plaque-forming units of respiratory syncytial virus induced significant or complete resistance to infection in both the upper and lower portions of the respiratory tract. this resistance did not appear to be the result of in vitro neutralization of virus during homogenization of tissue. virus was not recovered from the local site of inoculation after 5 min and was never detected in the respiratory tract of intramus ...1979572342
detection of respiratory syncytial virus in nasal secretions from infants by enzyme-linked immunosorbent enzyme-linked immunosorbent assay (elisa) was developed for detection of respiratory syncytial virus (rsv) in nasal secretions from infants with respiratory disease. rsv was detected in 23 of 29 secretions positive for rsv by tissue culture and in one of 36 samples negative for rsv by tissue culture. the elisa was a simple rapid, and surprisingly sensitive test for identification of rsv infection in infants.1979374650
nosocomial respiratory syncytial virus infections in an intensive care nursery: rapid diagnosis by direct immunofluorescence.a nosocomial outbreak of respiratory syncytial virus infections involved 8 of 17 infants in an intensive care nursery and one additional infant in the adjoining newborn nursery. immunofluorescent staining of nasopharyngeal specimens was positive in six of seven virologically confirmed cases (86%). one additional case with negative viral cultures was also identified by this technique. viral isolation in tissue cultures required an average of 4.9 days, whereas results of immunofluorescent studies ...1979382079
clinical and radiological studies in children with respiratory syncytial virus infection. 1979546771
long-term epidemiology of infections with mycoplasma pneumoniae.pneumonia due to mycoplasma pneumoniae was monitored in a large prepaid medical-care group in seattle, washington, between 1963 and 1975. the disease was diagnosed by isolation of m. pneumoniae and/or significant rises in titer of complement-fixing (antilipid) antibody in paired sera. infection was endemic without significant seasonal fluctuations. two epidemics occurred: the first peaked in january 1967, the second late in the summer of 1974. total rates of pneumonia infection in children incre ...1979448195
[serological survey for virus and mycoplasma infections (author's transl)].the authors describe the two years follow up of complement fixing antibodies vs 4 viral antigens (herpes simplex, herpes zoster, cytomegalovirus, respiratory syncytial virus, mycoplasma pneumoniae). they evaluate the seasonal trend and the eventual clinical correlations on 3267 sera and 254 spinal fluids from hospitalized patients.1979233415
clones of cells from a human embryo lung: their growth and susceptibility to respiratory viruses.colonies of cells were obtained from human fetal lung tissue and exposed to recently isolated respiratory viruses. there was a considerable variation in the number of rounded cells found in different colonies exposed to rhinovirus types 2 and 9 (rv2 and 9), human coronavirus 229e (hcv), adenovirus type 3 (ad3) and respiratory syncytial virus (rsv). smaller colonies had more rounded cells than larger colonies. clones were established from 9 out of 11 colonies. they varied in their rate of growth ...1979229795
comparison of direct immunofluorescent staining of clinical specimens for respiratory virus antigens with conventional isolation techniques.staining of clinical respiratory specimens obtained by nasopharyngeal and throat swabs with direct fluorescein isothiocyanate-conjugated antisera to para-influenza virus types 1, 2, and 3, influenza a virus, respiratory syncytial virus, adenovirus, mumps virus, and measles virus was compared with isolation procedures in routine tissue culture systems. direct staining of clinical specimens with the commercially available conjugated offered a rapid means of diagnosing respiratory infections on a r ...19806260834
respiratory syncytial virus. ii. isolation and morphology of the glycoproteins.treatment of respiratory syncytial (rs) virus with triton x-100 in solutions of low ionic strength solubilized not only glycoproteins but also some non-glycosylated proteins. rate zonal sedimentation of the solubilized materials resulted in separation of the glycoproteins from the other components, i.e. one fraction predominantly composed of two glycoproteins each with molecular weight (mol. wt.) of approximately 100,000 and 53,000 was obtained. electron microscopic observation of this fraction ...19806452027
cell cultures from bone affected by paget's disease.cells obtained from paget's bone specimens were maintained in culture for up to 8 1/2 months. the cells had several characteristics of bone cells including the presence of alkaline phosphatase, acid phosphatase, or succinic dehydrogenase as demonstrated histochemically. electron microscopy revealed nuclear inclusions similar to those found in the osterclasts of paget's disease in 4 or 11 cultures. immunohistology utilizing specific antisera demonstrated the presence of respiratory syncytial viru ...19806158956
rapid immunofluorescence diagnosis of respiratory syncytial virus infections among children in european countries. 19806104237
rapid diagnosis of respiratory syncytial virus infections in children. 19806105590
prospects for new viral vaccines.animal virology has made outstanding contributions to preventive medicine by the development of vaccines for the control of infectious disease in man and animals. cost-benefit analysis indicates substantial savings in health care costs from the control of diseases such as smallpox, poliomyelitis, yellow fever and measels. areas for further development include vaccines for influenza (living, attenuated virus), the herpes group (varicella: cytomegalovirus), respiratory syncytial virus, rotavirus a ...19806106250
seasonal pattern in childhood viral lower respiratory tract infections in melbourne.respiratory syncytial virus and parainfluenza viruses are the major pathogens in acute lower respiratory infection in infants and younger children. they show distinct seasonal patterns. an annual epidemic of respiratory syncytial virus infection is seen in melbourne and this coincides with the coldest months of the year. parainfluenza virus type 1, the most frequent cause of laryngotracheobronchitis, occurs as an autumn epidemic every second year. parainfluenza virus types 2 and 3 are present mo ...19806244480
inhibition of respiratory syncytial virus by bis(5-amidino-2-benzimidazolyl)methane. 19806247826
[rapid diagnosis of respiratory virus infections in children. respiratory syncytial virus demonstrated by the immunofluorescent technic]. 19806250259
experimental models for study of common respiratory viruses.numerous epidemiological studies have shown that there is excess respiratory disease morbidity in areas of high atmospheric pollution, implying an interactive effect on the clinical illness associated with these common infections. the principal etiologic agents of human respiratory infections are respiratory syncytial virus (rsv), influenza viruses (iv), parainfluenza virus types 1 and 3 (p1, p3), adenoviruses (ad), rhinoviruses (rv) and mycoplasma pneumoniae (mpn). understanding the pathogenesi ...19806250807
injury of respiratory epithelium.the large surface area provided by the respiratory tract epithelium of humans for exposure to microbial agents and toxic substances in the environment makes this organ system very vulnerable but a good early indicator of adverse health effects. however, the complexity of pulmonary defense mechanisms complicates definition of the interactive effects of pollutants and infectious agents. tracheal organ culture has been utilized to maintain organized, differentiated respiratory epithelium in vitro. ...19806250814
rheumatoid factor in acute viral infections: interference with determination of igm, igg, and iga antibodies in an enzyme immunoassay.igm rheumatoid factor (rf), an autoantibody to the fc fragment of igg, was determined by solid-phase enzyme immunoassay (eia). rf levels were significantly higher in patients with rubella virus infection than in patients with infections due to influenza virus, cytomegalovirus, respiratory syncytial virus, parainfluenza virus, adenovirus, mumps virus, or herpes simplex virus. to evaluate the role of rf in eia determinations of viral antibodies, igm rf from igm-igg cryoglobulin or control igm was ...19806251140
secretory immunological response in infants and children to parainfluenza virus types 1 and 2.the secretory immunological responses to natural infection with parainfluenza viruses ae not well defined. nasopharyngeal secretion specimens from 20 infants and children naturally infected with parainfluenza virus type 1 or type 2 were examined for class-specific antibody and virus-neutralizing activity. there was a marked discordance in individual secretions between immunoglobulin a (iga) antibody (as measured by indirect immunofluorescence) and neutralizing activity (as determined by either h ...19806254881
nosocomial viral infections: ii. guidelines for prevention and control of respiratory viruses, herpesviruses, and hepatitis viruses.this article reviews the most likely mechanisms of transmission of the commonly encountered respiratory viruses (influenza, respiratory syncytial virus, parainfluenza, rhinovirus), herpesviruses, and hepatitis viruses, and presents the guidelines used currently for prevention and control that are in use at strong memorial hospital.19806256306
a survey of virus infections of the respiratory tract of cattle and their association with disease.a total of 1590 caves were investigated between may 1972 and december 1975. twenty-two per cent were treated for respiratory disease and 2 . 5% died of pneumonia. almost 80% of the respiratory illness occurred in six sharp outbreaks. samples of virology were collected routinely from 127 healthy calves and from 354 calves treated for respiratory signs and comprised 1143 nasopharyngeal swabs and 1069 sera. virus infections were detected on 540 occasions including 135 by parainfluenzavirus type 3 ( ...19806256435
nosocomial viral infections: i. epidemiology and significance.viral illnesses in strong memorial hospital were monitored over a 17-month period. using criteria based primarily on the incubation periods for a number of common virus infections, the infections we found were classified as hospital- or community-acquired. hospital-acquired viral infections occurred on most hospital services; the majority of infections occurred on the pediatric and psychiatric services. infections due to herpesviruses were seen more frequently in a group of patients aged 14 year ...19806274823
disinfecting endoscopes: how not to transmit mycobacterium tuberculosis by bronchoscopy.mycobacterium tuberculosis was cultured from the bronchial washings of two patients who underwent bronchoscopy consecutively with the same bronchoscope. active pulmonary tuberculosis was later confirmed in the first patient, whereas the second patient had clinical and serologic evidence of infection with respiratory syncytial virus. the bronchoscope had been cleaned with an iodophor disinfectant, which had not destroyed the tubercle bacilli. the agent recommended for chemical disinfection of fib ...19806790150
effect of viral infections on pulmonary function in patients with chronic obstructive pulmonary diseases.the effect of 100 separate viral infections of the respiratory tract on pulmonary function was evaluated prospectively over an eight-year period in 84 patients with chronic obstructive pulmonary diseases and in eight normal subjects. some viral infections were associated with small acute declines in forced vital capacity and/or 1-sec forced expiratory volume of 25-300 ml. these declines were detectable only during the 90-day period after infection. the greatest abnormalities of pulmonary functio ...19806767794
breast-feeding and respiratory syncytial virus infection.the pattern of breast-feeding in 127 infants admitted to hospital with respiratory syncytial virus infection was compared with that in 503 age-matched controls. thirty per cent of children with infection had been breast-fed compared with 49% of controls. the approximate relative risk of being admitted to hospital with respiratory syncytial virus infection if not breast-fed was 2.2. several other factors were also considered, including an assessment of maternal care and home environment; the moth ...19806775754
microbiology of otitis media.the microbiology of otitis media (om) has been documented by cultures of middle ear fluid (mef) obtained by needle aspiration. the results of studies of bacteriology of om from sweden, finland and the united states are very similar: streptococcus pneumoniae and haemophilus influenzae are the most important pathogens: gram-negative enteric bacilli are isolated from mef of approximately equal to 20% of infants to six weeks of age but are rarely present in mef of older children; group a beta hemoly ...19806778362
[respiratory syncytial virus in cancer adult patients (author's transl)]. 19806937078
serodiagnosis of respiratory synctial virus infections in infants and young children by the immunoperoxidse technique.the immunoperoxidase antibody (ipa) technique was used to develop two new tests for serodiagnosis of respiratory syncytial virus infections: a microneutralization test based on the reduction of the number of infected cells and an ipa test for determination of virus-specific immunoglobulin g (igg). neutralizing antibody was determined both in the presence and absence of complement. in a group of 24 infants and young childres, ages less than 1 to 36 months, with acute respiratory syncytial virus i ...19806986407
prevention of infections with respiratory syncytial virus: the hopes and hurdles ahead.control of infections due to respiratory syncytial virus (rsv) by immunization poses special problems. first, the peak period of serious illness due to rsv is during the first few months of life, and thus a vaccine would have to be administered during the neonatal period. second, we understand little of the pathogenesis of and immunity to rsv disease in newborns, and an immune reaction may even play a role in the development of the lower respiratory tract disease seen in infancy. third, immunity ...19806997967
use of temperature-sensitive and cold-adapted mutant viruses in immunoprophylaxis of acute respiratory tract disease.efforts currently are underway to develop mutations in the influenza a viral genome that will bring about a satisfactory level of attenuation and that can be identified by simple in vitro techniques. two types of donor viruses that bear such mutations are being evaluated. one donor virus possesses temperature-sensitive (ts) mutations on the p1 and p3 genes, while the other donor bears both ts and cold-adaptation (ca) mutations. the mutant genes from these donors were transferred by gene reassort ...19806997969
immunopathologic mechanisms in lower respiratory tract disease of infants due to respiratory syncytial virus. 19806999535
bovine respiratory syncytial virus in quebec: antibody prevalence and disease outbreak.the prevalence of antibody to bovine respiratory syncytial virus in quebec and the role of the virus in a respiratory disease outbreak was investigated. the indirect immunofluorescent, neutralization and haemagglutination inhibition techniques were used to carry out this study. of the 1,444 adult animals examined 519 (35.9%) had antibody to bovine respiratory syncytial virus. these positive reactors were found in each agricultural region of quebec. the highest (53.0%) and the lowest (21.8%) prev ...19807000320
rubella immunity testing by mixed hemadsorption.a mixed hemadsorption (mh) test for naturally acquired rubella immunity and immune responses after rubella vaccination has been compared with the hemagglutination-inhibition (hi) test, the immunodiffusion (id) test, the neutralization test (nt), the hemolysis-in-gel (hig) test and the indirect immunofluorescence (if) test. the mh test was approximately equivalent to the hi test and the nt as to sensitivity for the early response to rubella vaccination, and all three tests are thought to measure ...19807003020
rapid diagnosis of respiratory syncytial virus infections by immunofluorescence on cell smears from nasopharyngeal secretions.the efficiency of indirect immunofluorescence for detection of respiratory syncytial (rs) virus antigens in nasopharyngeal secretions was compared with virus isolation on highly sensitive cell culture systems maintained in serum-free media. twenty out of 22 (91%) culture-positive specimens were correctly identified by indirect immunofluorescence, which gave no false positive results. since clinical specimens were sometimes unsuitable for immunofluorescence studies, this could explain two false n ...19807006628
[critical considerations on the laboratory research in infections from respiratory syncytial virus (rsv) (author's transl)]]. 19807008714
respiratory syncytial virus serology by a simplified enzyme-linked immunosorbent assay.a simplified enzyme-linked immunosorbent assay (elisa) which utilized commercially available reagents was developed for respiratory syncytial virus (rsv)-specific immunoglobulin g. an analysis of the inherent variation of the assay allowed the setting of strict criteria for determining a significant change in rsv antibodies. the elisa was more sensitive than the standard complement fixation or microneutralization tests in a carefully studied group of 32 rsv-infected adults. the elisa correlated ...19807012179
a simple and rapid microassay for the titration of human respiratory syncytial virus.a microassay, using tissue culture microplates for the titration of human respiratory syncytial virus by syncytium formation, is described. virus titers obtained agreed well with those obtained in a larger assay system; the microassay, however, is more rapid and economical. large numbers of virus samples are easily and rapidly processed as the assay necessitates an incubation period of only three days.19807014578
secretion of respiratory syncytial virus inhibitors and antibody in human milk throughout lactation.neutralising inhibitors to respiratory syncytial (rs) virus have been demonstrated in the whey of most samples of human milk tested. although high titres were secreted in colostra of some mothers (1/10-1/2,560; median 1/40) inhibitor levels in milk collected after the first week of lactation were uniformly low (median 1/10). high neutralising titres correlated with high colostral levels of specific antiviral iga but, unlike neutralising activity, iga antiviral antibody persisted in the milk of o ...19807014782
Displaying items 401 - 500 of 11972