Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| effect of sucrose phosphate and sorbitol on infectivity of enveloped viruses during storage. | cytomegalovirus and varicella-zoster virus recovery from sucrose phosphate (0.2 m sp) and 70% sorbitol (sorbitol) was compared after storage at -70, 4, and 20 degrees c over time. recovery from 0.2 m sp was uniformly better. more tissue culture infective doses and infectious foci were recovered in cell monolayers inoculated with 0.2 m sp virus stocks as compared with viruses stored in 70% sorbitol. although both viruses were isolated from diluted fresh stocks (10(-1) through 10(-4], freezing dil ... | 1983 | 6313747 |
| [contribution of direct immunofluorescence diagnosis to the epidemiology of acute viral respiratory infections in young children]. | rapid diagnosis by immunofluorescence (if) has enabled us to study the epidemiology of acute viral respiratory diseases in infants admitted to the chru at caen between january 1980 and december 1982. 809 nasal aspiration samples were examined. respiratory syncytial virus (rsv) was recovered in 36% of cases. these were observed during the four winter outbreaks which occurred during the study period and were extensive in 1980-1981 and 1982-1983. a virus was demonstrated in 13.5% of the 515 rsv neg ... | 1983 | 6316549 |
| [viruses and asthma]. | more than one-third of infants with respiratory syncytial virus (rsv) bronchiolitis subsequently experience recurrent episodes of wheezing. in asthmatic children, viral infections frequently trigger asthmatic attacks. indeed, viruses induce immunological and respiratory functional disorders. as compared with non-wheezing rsv infected infants, wheezing infants with bronchiolitis or asthma due to rsv have: a) increased t-lymphocyte responsiveness to rsv antigen; b) more persistent ige bound to nas ... | 1983 | 6320377 |
| [a survey on antibodies to respiratory syncytial virus in the sera of children in beijing]. | 1983 | 6226398 | |
| bronchiolitis: a clinical review. | bronchiolitis is an acute viral infection primarily caused by respiratory syncytial virus, affecting children under 2 years of age with a peak prior to six months. the clinical picture results from an inflammatory process of the small bronchi and bronchioles. infants present with tachypnea and wheezing often accompanied by respiratory distress and hyperinflation. supportive care is essential, children respond slowly to hydration and careful observation. respiratory distress requires hospitalizat ... | 1983 | 6389670 |
| mechanisms of immunity to respiratory syncytial virus in cotton rats. | active immunity and maternally transmitted passive immunity to respiratory syncytial virus (rsv) were studied in cotton rats. animals infected with respiratory syncytial virus developed complete resistance to pulmonary reinfection, which lasted at least 18 months. nasal resistance was of shorter duration and began to diminish in 8 months. pulmonary resistance was transferred by parabiosis, but nasal resistance was not. adoptive transfer studies with fractionated convalescent blood showed that se ... | 1983 | 6352505 |
| rapid diagnosis of respiratory syncytial virus and influenza a virus infections by immunofluorescence: experience with a simplified procedure for the preparation of cell smears from nasopharyngeal secretions. | a simplified procedure for preparing direct smears of exfoliated cells from nasopharyngeal secretions is presented. the specimens were examined by the indirect immunofluorescence (if) technique for the presence of respiratory syncytial (rs) virus and influenza a virus antigens. specimens from 457 children hospitalised at twelve different hospital departments in norway were examined during the winter season 1980-81. rs virus was identified in samples from 139 children and influenza a virus from 2 ... | 1983 | 6353858 |
| experimental respiratory syncytial virus pneumonia in young calves: microbiologic and immunofluorescent findings. | young calves were inoculated with respiratory syncytial virus (rsv) intranasally or by a combined intranasal and intratracheal route and were killed between postinoculation (initial) days (pid) 1 and 14. viral antigens were detected by immunofluorescence in nasopharyngeal cells from calves killed between pid 2 and 10. evidence of infection of the trachea and lungs with rsv was obtained by immunofluorescence and virus isolation in calves inoculated by the combined route, but not in calves inocula ... | 1983 | 6354017 |
| correlation of cytotoxic activity in lungs to recovery of normal and gamma-irradiated cotton rats from respiratory syncytial virus infection. | cotton rats (sigmodon hispidus) that were exposed to 300, 600, or 900 rads of gamma irradiation and inoculated intranasally 2 days later with respiratory syncytial virus (rsv) exhibited prolonged virus shedding and delayed humoral and cytotoxic immune responses compared with comparably inoculated nonirradiated control rats. in nonirradiated animals and in animals exposed to 300 and 600 rads, levels of virus declined and then disappeared from the lungs during the period in which cytotoxic activit ... | 1983 | 6354026 |
| some regularities in variations of respiratory syncytial virus strains during their epidemiological circulation. | among respiratory syncytial (rs) viruses circulating in the territory of u.s.s.r., strain variations were observed based on the differences in activity of virus reproduction in human embryo kidney cell cultures at 39 degrees c and in virus sensitivity to antibodies. mathematical expression of the correlation between these two genetic markers made it possible to classify the new isolates of rs virus into three groups differing in their virulence, namely high, mild and low virulent strains. popula ... | 1983 | 6138987 |
| rapid immunoperoxidase assay for detection of respiratory syncytial virus in nasopharyngeal secretions. | samples of nasopharyngeal secretions obtained from 70 infants and young children with acute respiratory disease were examined for the presence of respiratory syncytial virus by immunoperoxidase assay (ipa). the ipa was compared with the immunofluorescence assay and with cell culture isolation. respiratory syncytial virus antigen-positive cells were detected by both ipa and immunofluorescence assay in 28 specimens; 25 samples were positive in cell culture. the agreement between virus isolation an ... | 1983 | 6355168 |
| monoclonal antibodies to respiratory syncytial virus proteins: identification of the fusion protein. | six monoclonal antibodies directed against respiratory syncytial virus proteins were produced. each was characterized by immunoprecipitation and indirect immunofluorescence. one was directed against the nucleocapsid protein. np 44, two were directed against a 37,000-dalton protein, two were directed against the major envelope glycoprotein, gp 90, and one was directed against the 70,000-dalton envelope protein, vp 70. indirect immunofluorescence stain patterns of infected hep-2 cells defined gp 9 ... | 1983 | 6345804 |
| cell-mediated cytotoxic responses in lungs of cotton rats infected with respiratory syncytial virus. | pneumonia induced in cotton rats (sigmodon hispidus) after inoculation of respiratory syncytial virus (rsv) was accompanied by the appearance of leukocytes in infected lungs. the number of these leukocytes increased until day 5 after inoculation when sixfold more leukocytes were recovered from infected lungs using transpleural lavage than were recovered from lungs of uninfected control animals. although macrophages and lymphocytes constituted approximately 65 and 25%, respectively, of the cells ... | 1983 | 6340570 |
| viruses associated with acute respiratory infections in children admitted to hospital in naples, 1979-82. | a survey of the virological and epidemiological features of acute respiratory diseases in children admitted to hospital in naples has been carried out; the results of three years of research are reported.between april 1979 and march 1982, 787 nasopharyngeal swabs were examined. there were 287 (36.5%) positive samples, with the highest isolation rate being found in children with bronchiolitis (39.5%).among the different viruses isolated, adenovirus was the most common (161 positive samples, 56%); ... | 1983 | 6325032 |
| ribavirin for influenza and respiratory syncytial virus infections. | 1983 | 6136701 | |
| monoclonal antibodies protect against respiratory syncytial virus. | 1983 | 6138541 | |
| cdna cloning and transcriptional mapping of nine polyadenylylated rnas encoded by the genome of human respiratory syncytial virus. | we have isolated cdna clones representing nine unique poly(a)+ rnas transcribed from the genome of human respiratory syncytial virus, a paramyxovirus. a cdna library was constructed by using poly(a)+ rna from virus-infected cells as template and the escherichia coli plasmid pbr322 as vector. viral cdna clones were identified by hybridization with cdna probes prepared from viral genomic rna. the viral clones were grouped into nine different families by hybridization with individual size-selected ... | 1983 | 6190173 |
| report of a workshop on respiratory syncytial virus and parainfluenza viruses. | 1983 | 6194232 | |
| antiviral drugs 1983. | a number of antiviral compounds are currently available, and several others are of great interest. trifluridine, idoxuridine, and vidarabine are effective topically in herpes simplex virus keratoconjunctivitis infection. vidarabine (and presumably acyclovir) is effective in herpes simplex virus encephalitis and in herpes zoster infections in the immunocompromised host. acyclovir is effective topically, orally, and intravenously in primary herpes genitalis, and the oral and intravenous forms are ... | 1983 | 6194388 |
| antigenic and biological criteria of differentiation of the newly isolated strains of respiratory syncytial virus. | respiratory syncytial (rs) virus strains isolated in different years varied by their antigenic and biological properties. the lowest degree of relatedness was found between the "street" virus and the prototype long strain; the highest occured among the isolates from a given isolation period. based on the mean indices of efficiency of the virus reproduction in human embryo lung (hel) cells at 37 degrees c and 39 degrees c as well as on the degree of virus sensitivity to reference antibodies, the ... | 1983 | 6195898 |
| respiratory syncytial virus infection in syrian hamsters. i. development of humoral immunity. | adult syrian hamsters have been shown useful for the study of respiratory syncytial virus (rsv) infection. clinical manifestations coincided with the maximum replication of the virus in respiratory tract of infected animals as confirmed by immunofluorescence (if) test. the dynamics of secretory and serum antibody responses is described in infected and reinfected animals. | 1983 | 6140834 |
| respiratory syncytial virus infection in syrian hamsters. ii. regulation of the immune response. | the dynamics of immune cell responsiveness in the process of development of primary and secondary immune responses after infection of adult syrian hamsters with native respiratory syncytial virus (rsv) was studied. in primary immune response, the enhancement of functional responsiveness of lymphocytes to mitogens and the maximum level of their spontaneous proliferative activity were found to precede the optimal level of antibody synthesis. regulation of the dynamics of immunogenesis after reinfe ... | 1983 | 6140835 |
| aromatic amidines: comparison of their ability to block respiratory syncytial virus induced cell fusion and to inhibit plasmin, urokinase, thrombin, and trypsin. | two series of amidine derivatives consisting of a total of 24 compounds were examined for a correlation between their blocking effect on respiratory syncytial virus induced cell fusion and their inhibitory activity against selected trypsin-like protease. although no correlation was evident between the two activities, several potentially important discoveries were made. a highly selective inhibitor of plasmin over thrombin (compound 10) was obtained, and a potent new blocker of virus-induced cell ... | 1983 | 6219223 |
| enhancement of respiratory syncytial virus-induced cytopathology by trypsin, thrombin, and plasmin. | a series of proteases of diverse substrate specificity were tested for their effect on respiratory syncytial virus-induced cytopathology. three of the enzymes, thrombin, plasmin, and trypsin, were able to augment significantly the fusion of virus-infected a549 cells. on a concentration basis, thrombin was the most active promoter, followed by plasmin and then trypsin. hirudin, a specific thrombin inhibitor, blocked the fusion-enhancing property of thrombin, yet had no influence on the basal rate ... | 1983 | 6219957 |
| defective regulation of immune responses in respiratory syncytial virus infection. | the relationship of suppressor cell numbers and function to virus-specific ige response was determined in 72 infants with respiratory syncytial virus (rsv) infection. monoclonal antibodies to membrane antigens were used to enumerate okt4 and okt8 antigen-positive cells, and suppressor cell function as quantitated by the degree of suppression of lymphocyte mitogenesis induced by incubation of lymphocyte cultures with histamine. patients with bronchiolitis had fewer okt8-positive cells during conv ... | 1984 | 6236259 |
| monoclonal antibodies protect against respiratory syncytial virus infection in mice. | twenty-five monoclonal antibodies (mab) to respiratory syncytial virus (rsv) and two to hepatitis b virus were inoculated intravenously into mice. twenty-four hours later the mice were challenged intranasally with rsv. eleven of 14 mab against fusion protein and four out of six mab against a larger glycoprotein (gp84) significantly reduced the titre of rsv in the lungs when mice were killed 5 days later. five mab against three other rsv proteins and two mab against hepatitis b virus had no signi ... | 1984 | 6201436 |
| antiviral effects of bovine interferons on bovine respiratory tract viruses. | the antiviral effects of bovine interferons on the replication of bovine respiratory tract viruses were studied. bovine turbinate monolayer cultures were treated with bovine interferons and challenged with several bovine herpesvirus 1 strains, bovine viral diarrhea virus, parainfluenza type 3 virus, goat respiratory syncytial virus, bovine respiratory syncytial virus, bovine adenovirus type 7, or vesicular stomatitis virus. treatment with bovine interferons reduced viral yield for each of these ... | 1984 | 6201505 |
| monoclonal antibodies to human respiratory syncytial virus and their use in comparison of different virus isolates. | monoclonal antibodies to the rsn -2 isolate of human respiratory syncytial (rs) virus have been characterized with regard to their specificity for viral polypeptides and for different rs virus isolates. five hybridoma antibodies recognized the phospho-protein vpp32 and the other recognized the matrix protein vpm27 . evidence was obtained to support the view that vpp32 was associated with the nucleoprotein vpn41 . three of the antibodies to vpp32 showed cytoplasmic immunofluorescent staining whil ... | 1984 | 6202832 |
| monoclonal antibodies for the rapid diagnosis of respiratory syncytial virus infection by immunofluorescence. | eight separate monoclonal antibodies to the long strain of respiratory syncytial virus (rsv) were tested for their utility as rapid diagnostic reagents in immunofluorescence. preliminary screening indicated that all 8 reacted with 11 field strains from three previous local rsv outbreaks and with 4 of 5 additional strains chosen because of their antigenic diversity by neutralization. two monoclonal antibodies, one each directed against a surface glycoprotein and the nucleocapsid protein, were the ... | 1984 | 6204807 |
| spectrophotometric quantitation of peroxidase-stained protein bands following gel electrophoresis and the western blot transfer technique with respiratory syncytial virus. | a procedure is described for spectrophotometrically measuring the position and intensity of elisa-stained protein bands after western blot analyses. partially purified virus proteins separated by acrylamide gel electrophoresis and electrophoretically transblotted onto nitrocellulose paper were identified by elisa reactions according to established procedures. the nitrocellulose paper strips were then rendered transparent on glass slides by treatment with a plastics solvent (gelman sepra-clear). ... | 1984 | 6205014 |
| antigenic and structural variation in the major nucleocapsid protein of respiratory syncytial virus. | antigenic and structural variation in the major nucleocapsid protein, vpn41, from different strains of respiratory syncytial (rs) virus was observed using a combination of monoclonal antibodies and two-dimensional peptide mapping. limited trypsin treatment of intact nucleocapsids produced two peptide fragments mr 27k and mr 14k. two monoclonal antibodies, n1 and n2, reactive with primary sequence epitopes located on intact nucleocapsids also reacted with either the 27k fragment (n2) or the 14k f ... | 1984 | 6208317 |
| the characterization and uses of monoclonal antibodies to respiratory syncytial virus. | fifteen hybridomas secreting monoclonal antibodies against respiratory syncytial virus (rsv) have been produced. three react with nucleoprotein (mw 42 000 daltons), 1 with phosphoprotein (mw 36 000 daltons), 1 with a larger protein (mw 60 000 daltons) and 10 with the fusion glycoprotein (mw 46 000 + 22 000 daltons). by immunofluorescent staining of infected cells, 5 monoclonal antibodies bind to cytoplasmic inclusions and the remainder give diffuse cytoplasmic fluorescence. virus infectivity is ... | 1984 | 6084614 |
| detection of respiratory virus antigens in nasopharyngeal secretions from patients with acute respiratory disease by radio-immunoassay and tissue culture isolation. | an investigation was made of the sensitivity and specificity of four-layer radio-immunoassays (ria) in the detection of adenovirus, respiratory syncytial virus, influenza virus types a and b, as well as para-influenza virus types 1, 2 and 3 from nasopharyngeal aspirates of 146 patients with acute respiratory disease. the sensitivity of ria was comparable with that of tissue culture isolation if the total number of positives is considered. the difference may have been caused both by a higher effi ... | 1984 | 6088961 |
| prevalence of antibodies to bovine respiratory syncytial virus, bovine viral diarrhea virus, bovine herpesvirus-1, and bovine parainfluenza-3 virus in sheep and goats in quebec. | serum samples were collected from 1,075 clinically normal sheep and goats from 77 flocks in 7 agricultural regions of quebec from june to august 1982. sheep and goats were tested for antibodies to bovine respiratory syncytial virus, bovine viral diarrhea virus, and bovine herpes-virus-1 by the indirect fluorescent antibody technique and for parainfluenza-3 virus by the hemagglutination inhibition test. the prevalence of antibodies in animals to respiratory syncytial virus was 31%; to bovine vira ... | 1984 | 6089626 |
| survey for antibodies to respiratory viruses in two groups of sheep in northern ireland. | two hundred serum samples from texel and texel crossbred sheep (non-indigenous breeds) and 200 from indigenous northern ireland breeds (mainly blackface, cheviot and border leicester crosses) were tested for antibodies to parainfluenza virus types 1, 2 and 3, respiratory syncytial virus, bovine adenovirus (subgroups 1 and 2), influenza type a, maedi-visna virus and bovine virus diarrhoea virus. the percentage of animals with antibodies to parainfluenza virus 3 (50 to 56 per cent) and adenovirus ... | 1984 | 6095511 |
| serological studies of parainfluenza type 3 virus, bovine adenovirus type 3 and bovine respiratory syncytial virus infection in beef calves. | six serum samples were taken at monthly intervals from birth to weaning from each of 41 newborn calves in the autumn and spring calf crops of a beef cow--calf herd. the serum hemagglutination-inhibition (hi) antibody titres to parainfluenza type 3 virus (piv-3), virus-neutralization (vn) antibody titres to bovine adenovirus type 3 (bav-3) and bovine respiratory syncytial virus (brsv) were determined using microtitration techniques. there was serological evidence of a significantly higher inciden ... | 1984 | 6095517 |
| nucleotide sequence of the gene encoding the fusion (f) glycoprotein of human respiratory syncytial virus. | the nucleotide sequence of the mrna encoding the f protein of respiratory syncytial (rs) virus (strain a2) was determined from cdna clones that contain the complete mrna sequence. the mrna is 1899 nucleotides long exclusive of polyadenylylate. the single major open reading frame encodes a protein of 574 amino acids, with a calculated molecular weight of 63,453. major structural features predicted from the amino acid sequence include an nh2-terminal signal sequence (residues 1-22), hydrophobic tr ... | 1984 | 6096849 |
| prevention of bovine respiratory syncytial virus infection and clinical disease by vaccination. | a double blind field trial was carried out with a live attenuated bovine respiratory syncytial virus vaccine. the trial involved 530 calves, two to 10 months old, on 27 dairy farms, where respiratory problems due to bovine respiratory syncytial virus infections had been observed during the preceding year. in 17 herds either all calves were vaccinated (nine groups) or all calves received a placebo (eight groups). in 10 herds half the number of calves were vaccinated and the other half kept as non ... | 1984 | 6097014 |
| a simultaneous outbreak of respiratory syncytial virus and parainfluenza virus type 3 in a newborn nursery. | over a 3-week period, 20 of 34 (59%) infants in a newborn nursery developed nosocomial viral respiratory tract disease. either respiratory syncytial virus (seven infants) or parainfluenza virus type 3 (five) or both (two) were demonstrated in respiratory secretions from 14 of the 20 symptomatic patients. symptoms in the 20 infants included rhinitis (15 infants), cough (14), apnea (eight), pulmonary infiltrates (seven), and fever (six). there were no differences in symptoms between children infec ... | 1984 | 6325655 |
| brs virus, pi3 virus and bhv1 infections of young stock on self-contained dairy farms: epidemiological and clinical findings. | the role of bovine respiratory syncytial virus, parainfluenza type 3 virus and bovine herpesvirus 1 as disease agents in 28 groups of young cattle on 19 dairy farms which raised their own replacements was investigated. bovine respiratory syncytial virus infections occurred in 27, parainfluenza type 3 virus infections in all and bovine herpesvirus 1 infections in three of the 28 groups. some infections were accompanied by clinical signs while others were entirely subclinical. clinical respiratory ... | 1984 | 6326374 |
| respiratory virus infections and aeroallergens in acute bronchial asthma. | two hundred and fifty six attacks of acute bronchial asthma occurring in 169 children aged over 2 years were studied during a two year period. more attacks occurred during spring and autumn than at other times of the year. in 73 patients (29%) a respiratory virus infection was diagnosed, with the same seasonal variation as the asthmatic attacks. most of the virus infections were caused by rhinovirus (45%) and respiratory syncytial virus (19%). there was no significant correlation between asthmat ... | 1984 | 6326694 |
| prevalence of bovine herpesvirus-1, bovine viral diarrhea, parainfluenza-3, goat respiratory syncytial, bovine leukemia, and bluetongue viral antibodies in sheep. | sera from healthy sheep were collected in january and march 1982 from flocks of sheep located in southwestern and southeastern louisiana. these sera were tested for bovine herpesvirus-1 (bhv-1), bovine viral diarrhea virus (bvdv), parainfluenza-3 (pi-3) virus, and goat respiratory syncytial virus (grsv) antibodies by microtitration virus-neutralization test. the sera were tested also for bovine leukemia virus (blv) and bluetongue virus (btv) antibodies by immunodiffusion tests. the number of flo ... | 1984 | 6329044 |
| an enzyme-linked immunosorbent assay (elisa) for igg and iga antibodies to respiratory syncytial virus in low dilutions of secretions of human serum and secretions. | an enzyme-linked immunosorbent assay (elisa) has been developed for titration of igg and iga antibodies to respiratory syncytial (rs) virus in low dilutions of human serum, colostrum, and nasopharyngeal secretions. previously the sensitivity of rs virus elisa on such specimens has been limited by nonspecific absorption of antibody, particularly iga, to crude antigen preparations. for igg antibody estimation in infant sera, this unwanted binding was reduced to workable levels by increasing the se ... | 1984 | 6389773 |
| antibiotic treatment of pneumonia and bronchiolitis. a prospective randomised study. | routine administration of antibiotics in the treatment of pneumonia and bronchiolitis in infants and small children was evaluated in an open randomised prospective trial. from 1979-82 136 children between the age of 1 month and 6 years were allocated to one of two treatment groups shortly after their admission to a paediatric ward. group a patients were to be given antibiotics but those in group b were not. none of the children had received antibiotics before hospital admission. a viral infectio ... | 1984 | 6391389 |
| respiratory virus infection in hospitalized children in austria 1979-1982. diagnosis by immunofluorescence. | nasal secretions from 349 austrian children under six years of age who were hospitalized for respiratory illnesses were screened for the presence of respiratory syncytial virus (rsv), parainfluenza virus 1 and 3, adenovirus and influenza a virus over a period of four years by the immunofluorescence technique. 35% of the specimens were found to be positive for one of the five viruses investigated. rsv was detected in 31% of the nasal secretions and was thus the most frequently encountered causati ... | 1984 | 6381315 |
| cell-mediated immune response in cattle to bovine respiratory syncytial virus. | cattle inoculated with bovine respiratory syncytial virus (brsv) were evaluated for the development of a cell-mediated immune response. results of the leukocyte migration-inhibition test under agarose and the delayed hypersensitivity test indicated that a cell-mediated immune response was elicited after intranasal inoculation of calves with brsv. migration inhibition in the leukocyte migration-inhibition test was detected by postinoculation day (pid) 5 and reached maximum inhibition on pid 21. i ... | 1984 | 6383149 |
| pathogenesis of experimental bovine respiratory syncytial virus infection in sheep. | conventionally reared lambs, seronegative to bovine respiratory syncytial virus (rsv), were inoculated with bovine rsv by an aerosol route. a mild clinical response, characterized by transient fever at postinoculation day (pid) 4, occurred and was accompanied by serous oculonasal secretions in less than 50% of the infected lambs. a decrease in total wbc was observed on pid 6 and 11, with a reduction in the total numbers of lymphocytes. bovine rsv was recovered from nasal secretions of inoculated ... | 1984 | 6383151 |
| interaction of bovine respiratory syncytial virus and pasteurella haemolytica in the ovine lung. | the potential synergistic effect of bovine respiratory syncytial virus (rsv) and pasteurella haemolytica in the production of pneumonia after aerosol/intranasal infection of conventionally reared lambs was evaluated. a mild clinical response was observed in lambs given virus and/or bacteria. gross pulmonary lesions were seen in 3 of 6 lambs given rsv and then p haemolytica 3 or 6 days later, respectively (groups d and e), and in 1 lamb of 5 given virus and bacteria simultaneously (group g). gros ... | 1984 | 6383152 |
| detection by elisa of iga and igm antibodies in secretion and igm antibodies in serum in primary lower respiratory syncytial virus infection. | twenty-six infants and children with primary lower rs virus infection, diagnosed by the detection of rs virus in nasopharyngeal secretion (nps) by use of immunofluorescent antibody (fa) technique, were studied with respect to the presence of iga and igm antibodies. samples of nps and serum obtained during the first 3-4 months following the beginning of illness, were investigated. employing a reverse elisa technique, we found igm antibodies in the acute, but not during the convalescent, phase of ... | 1984 | 6363623 |
| infection of gnotobiotic calves with a bovine and human isolate of respiratory syncytial virus. modification of the response by dexamethasone. | a bovine and a human strain of rsv both adapted to bovine cell culture, have been inoculated separately into 13 and 7 gnotobiotic calves respectively by 3 different methods. both strains infected calves and showed similar growth patterns. virus was recovered from the nasopharynx between one and 11 days with peak titres between 3 and 8 days following inoculation. with the exception of 4 calves treated with dexamethasone no clinical signs and only minimal macroscopic lesions of the lung were induc ... | 1984 | 6365036 |
| experimental pneumonia in gnotobiotic calves produced by respiratory syncytial virus. | a bovine isolate of respiratory syncytial virus (rsv), when inoculated intranasally into eight gnotobiotic calves produced significant macroscopic lesions of the lung (2-25% consolidation) but failed to produce any clinical signs of disease. the microscopic lesions comprised proliferative and exudative bronchiolitis with accompanying alveolar collapse and infiltration by mononuclear cells of the peribronchiolar tissue and alveolar walls. virus was recovered from the nasopharynx between days 2 an ... | 1984 | 6365144 |
| evidence for both respiratory syncytial virus and measles virus antigens in the osteoclasts of patients with paget's disease of bone. | recent ultrastructural and immunohistochemical evidence supports the hypothesis that paget's disease of bone is a slow viral infection of the paramyxoviridae family. conflicting evidence for the presence of respiratory syncytial virus (rsv), a pneumovirus, or measles, a morbillivirus, has been reported. by the indirect fluorescent antibody assay, four rsv antisera were compared with four measles antisera on serial sections of pagetic bone or replicate coverslips of cells from pagetic bone grown ... | 1984 | 6365389 |
| [respiratory syncytial virus and influenza a virus infections in children. a simplified preparation of samples for rapid virus diagnosis]. | 1984 | 6369621 | |
| bronchopulmonary obstruction in children with respiratory virus infections. | among 873 children hospitalized with confirmed respiratory viral infections, 492 suffered from bronchopulmonary obstruction. respiratory syncytial virus infections were dominant among the patients with bronchopulmonary obstruction (80%). the majority of patients with rhinovirus infections suffered from bronchopulmonary obstruction (57%). two main groups of children at risk from developing bronchopulmonary obstruction during respiratory virus infections were found: 1) infants, suffering from bron ... | 1984 | 6321218 |
| treatment of respiratory viral infection in an immunodeficient infant with ribavirin aerosol. | an infant with severe combined immunodeficiency syndrome (scids) secondary to adenosine deaminase deficiency had pneumonitis and combined infection with respiratory syncytial virus (rsv) and parainfluenza virus type 3 (piv3). four separate courses of ribavirin were delivered by small-particle aerosol. the piv3 disappeared during the first course, and rsv disappeared after the fourth course on the 58th hospital day. neither virus returned during profound immunosuppression for bone marrow transpla ... | 1984 | 6322573 |
| a prospective study of chlamydial, mycoplasmal, and viral infections in a neonatal intensive care unit. | in a prospective study of non-bacterial infection in a neonatal intensive care unit in north west london, chlamydia trachomatis infection was identified in 4 of 280 babies (1.4%) and was the most common cause of neonatal ophthalmia. one of the four developed pneumonitis. ureaplasma urealyticum was found to colonise the nasopharynx in 53 of 235 babies (22.6%), with mycoplasma hominis present in 6 of 235 babies (2.6%). there was a statistically significant association between u urealyticum colonis ... | 1984 | 6322705 |
| [exanthematous viral diseases and measles: serology]. | a study of antibodies against measles virus and other viral agents is made to investigate possible existence of a false measles or "measles-like" in 48 children with this disease during an epidemic. hemagglutination-inhibiting technique was used to determine rubella antibodies, and complement fixation technique to measure those produced against other diseases studied. results were grouped according to the seroconversions found. the following serologic diagnoses were found: twenty eight cases of ... | 1984 | 6703539 |
| purification and characterization of gp90, one of the envelope glycoproteins of respiratory syncytial virus. | the large glycoprotein, gp90, of respiratory syncytial virus (rsv) was purified by affinity chromatography using a monoclonal antibody. hyperimmune rabbit antiserum directed specifically to the purified gp90 neutralized rsv to high titre but did not inhibit fusion of previously infected cells. 125i-labelled gp90 bound rapidly to hep-2 cells in an unsaturable manner; binding was inhibited by the hyperimmune rabbit antiserum. | 1984 | 6707612 |
| [isolation of the respiratory syncytial virus from calves with respiratory diseases]. | described is a respiratory enzootic in calves aged 30 to 90 days. two cytopathic strains of the bovine respiratory syncytial virus were isolated from diseased calves in a cell culture of lamb thyroid. clinical observations and the results of virologic and serologic investigations have revealed that mixed infections of the respiratory syncytial virus and the mucosa disease virus take part in the etiology of the respiratory enzootic described. | 1984 | 6730327 |
| an outbreak of respiratory syncytial virus infection in an old people's home. | at least 20 of the 50 residents of an old people's home suffered from an acute respiratory infection and four of them died. respiratory syncytial virus was isolated from two of the patients. the importance of this virus in the elderly is probably underestimated. | 1984 | 6736667 |
| some respiratory and enteric diseases of cattle: an update. | fibrinous pneumonia caused 40-45% of death losses in cattle shipped into bruce county, ontario. feeding corn silage within the first 2 weeks of arrival increased mortality. commingling cattle from different sources and keeping more than 110 cattle in a pen were detrimental. while use of antimicrobials in the starter ration was beneficial, prophylactic use of antimicrobials in the water was not. mortality was directly proportional to morbidity and treatment costs. vaccinating against any respirat ... | 1984 | 6738491 |
| nucleotide sequence of the gene encoding respiratory syncytial virus matrix protein. | the amino acid sequence of the matrix protein of the human respiratory syncytial virus (rs virus) was deduced from the sequence of a cdna insert in a recombinant plasmid harboring an almost full-length copy of this gene. it specifically hybridized to a single 1,050-base mrna from infected cells. the recombinant containing 944 base pairs of rs viral matrix protein gene sequence lacked five nucleotides corresponding to the 5' end of the mrna. the nucleotide sequence of the 5' end of the mrna was d ... | 1984 | 6699948 |
| pathogens associated with acute lower respiratory tract infection in young children. | to determine the agents associated with acute lower respiratory infection in young children, we studied 102 hospitalized children less than 5 years old using culture and serology for viruses and chlamydia trachomatis, fluorescent antibody testing for pertussis and respiratory syncytial virus, blood cultures and counterimmunoelectrophoresis of nasopharyngeal secretions and urine for pneumococcal and haemophilus influenzae type b antigens. at least one agent was detected in 87 children and multipl ... | 1984 | 6701101 |
| bovine respiratory syncytial virus infections in young dairy cattle: clinical and haematological findings. | clinical signs and haematological findings during the acute and convalescent phases of an infection with bovine respiratory syncytial virus (rsv) were studied under field conditions. the study was carried out in 139 cattle less than 16 months of age in 16 herds with serologically proven bovine rsv infections. blood was collected for serological and haematological examination. repeated clinical examinations were carried out until 35 days after appearance of disease. signs of general disease such ... | 1984 | 6702077 |
| respiratory syncytial virus infection in mice. | the a2 strain of human respiratory syncytial virus replicated in the nose and lung of balb/c mice, with virus growing to higher titers in older animals than in younger animals. virus was recovered from the nose between days 2 and 7 with peak titers on days 3 and 4, and from the lungs between days 2 and 9, with peak titers on days 4 through 6. serum antibody developed 2 weeks after infection. viral antigen was demonstrated in the alveolar cells of the lung by immunofluorescence. histopathological ... | 1984 | 6693171 |
| cell-mediated immunity in respiratory syncytial virus disease. | systemic cell-mediated responses to respiratory syncytial (rs) virus were detected, using a whole blood transformation assay, in 10 of 28 infants and children with rs virus infections during the period 1-14 days postadmission. cell-mediated responses were unrelated to the age of the patient or the severity of illness. no correlation was found between cellular responses and fourfold or greater rises in antibody titre to rs virus, as determined by a membrane immunofluorescence technique. patients ... | 1984 | 6693860 |
| giant cell pneumonia due to respiratory syncytial virus. occurrence in severe combined immunodeficiency syndrome. | a 6-month-old male infant with a severe combined immunodeficiency syndrome was hospitalized for progressive respiratory distress. examination during hospitalization disclosed widespread pulmonary infiltrates that did not respond to intensive therapy. the patient died eight days after admission. autopsy disclosed pneumocystis carinii pneumonia and widespread giant cell pneumonia. respiratory syncytial virus (rsv) was grown from a lung specimen obtained at autopsy. specific immunofluorescent stain ... | 1984 | 6611144 |
| long-term prospective study in children after respiratory syncytial virus infection. | we have prospectively evaluated for the past 8 years 29 children who were hospitalized during infancy with acute lower respiratory tract illness caused by respiratory syncytial virus (rsv). no differences in the prevalence of a family history of atopy or breast-feeding in these infants compared with controls were noted. however, a history of parental smoking was significantly associated with hospital admission for rsv lower respiratory tract disease. evidence of atopy, as defined by serum ige le ... | 1984 | 6470859 |
| isolation of pathogenic bacteria from the nasopharynx of children with respiratory syncytial virus infection. predictive value of chest roentgen examination and laboratory tests. | potentially pathogenic bacteria were isolated from the nasopharynx in 33/66 hospitalized infants and children with verified respiratory syncytial virus (rsv) infection. the value of chest roentgenograms and blood counts for the prediction of concomitant bacterial infection was evaluated. abnormal chest roentgenograms were found in 89% whether pathogenic bacteria were present or not. the most common finding was general hyperinflation of the lungs, with or without infiltrates. this occurred more f ... | 1984 | 6377479 |
| immune response to respiratory syncytial virus: prevention of syncytia formation by human serum during in vitro infection. | human serum specimens containing respiratory syncytial virus (rsv)-specific neutralizing antibody were found to prevent the formation of syncytia when applied to hep-2 tissue culture monolayers which had been infected with rsv 12 h previously. this was evidenced by the demonstration of rsv-infected cells without any syncytia formation in the monolayers treated with rsv antibody-positive serum. on the other hand, widespread syncytia formation was observed with antibody-negative control serum. the ... | 1984 | 6387611 |
| virus-induced complement activation and neutrophil-mediated cytotoxicity against respiratory syncytial virus (rsv). | complement-dependent neutrophil-mediated cytotoxicity (cdnc) was determined by specific release of 51-chromium (51cr) from respiratory syncytial virus infected hep2 cells in a microcytotoxicity assay. there was significant release of 51cr from rsv infected cells as compared to uninfected cells in the presence of complement (c) and neutrophils (pmn). the degree of cytotoxicity was dependent upon the concentration of c used in the assay. such cytotoxicity was effectively abolished after heat-inact ... | 1984 | 6744659 |
| comparison of immediate and delayed inoculation of hep-2 cells for isolation of respiratory syncytial virus. | immediate inoculation of hep-2 cells is generally advocated for the isolation of respiratory syncytial virus. however, delayed laboratory inoculation of properly transported specimens obtained by aspiration of nasopharyngeal mucus provided an isolation rate similar to that obtained with immediate inoculation. | 1984 | 6746883 |
| diagnosis of respiratory syncytial virus infection in children: comparison of viral antigen detection and serology. | direct detection of viral antigen in nasopharyngeal secretion by radioimmunoassay was compared with serology by igg antibody enzyme immunoassay for diagnostic efficacy in 77 children with clinically suspected respiratory syncytial virus (rsv) infections. antigen detection gave a positive diagnosis in 26 of 33 (79%) children in whom rsv infection was diagnosed by any of the two methods. the diagnostic efficacy of antigen detection was dependent upon the interval after onset at which specimens wer ... | 1984 | 6747612 |
| immunoglobulin class-specific antibody response in respiratory syncytial virus infection measured by enzyme immunoassay. | immunoglobulin class-specific enzyme immunoassay (eia) was used for determination of antibody responses in sera collected from 26 children with acute primary respiratory syncytial virus (rsv) infections. all 26 patients had igg antibody responses with a significant titer increase in 24 (92%); an igm antibody response was detected in 19 of the 26 (73%). from patients aged 6 months or less only 5 of 8 produced detectable igm antibodies, whereas all patients aged 1-2 years did so. igm antibodies ap ... | 1984 | 6747613 |
| respiratory syncytial virus diagnosis. | 1984 | 6372231 | |
| protection from respiratory syncytial virus infection in cotton rats by passive transfer of monoclonal antibodies. | the effects of passive administration of neutralizing monoclonal antibodies (mab) to respiratory syncytial virus glycoproteins (gp90 and vp70) was evaluated in cotton rats challenged with respiratory syncytial virus. animals injected with mab to vp70 had lower mean viral titers in lung tissues than did controls (log10 2.5 versus 5.4 pfu/g; p less than 0.001), as did cotton rats given mab to gp90 (log10 2.1 versus 5.0 pfu/g; p less than 0.001). fifty percent of animals given either mab had no det ... | 1984 | 6363300 |
| respiratory syncytial virus infection in the elderly. | 1984 | 6419871 | |
| management of respiratory failure in infants with acute viral bronchiolitis. | fifteen infants with acute viral bronchiolitis required mechanical ventilation. infants were all aged less than 12 weeks and all had evidence of atelectasis or pneumonia on chest x-ray films. respiratory syncytial virus was identified by immunofluorescence in 14 of 15 patients. intubation and mechanical ventilation were initiated to reduce the work of breathing rather than to treat hypoxemia alone. additional therapy included continuous positive airway pressure or positive end-expiratory pressur ... | 1984 | 6437212 |
| [rapid diagnosis of respiratory syncytial virus in children with bronchiolitis by indirect immunofluorescence technic]. | 1984 | 6400166 | |
| viral diagnoses using the rapid immunofluorescence technique and epidemiological implications of acute respiratory infections among children in different european countries. | from november 1978 to october 1981, a total of 7716 specimens of nasopharyngeal secretions were examined by the rapid immunofluorescence technique to determine the frequency of infections caused by the respiratory syncytial virus (rsv), influenza virus a, and parainfluenza viruses 1 and 3. the tests were carried out in six different virus laboratories located in newcastle upon tyne (england), copenhagen, oslo, stockholm, turku (finland), and vienna; laboratories in lisbon and paris participated ... | 1984 | 6375886 |
| a comparison of three vaccines against respiratory syncytial virus in calves. | an inactivated vaccine against respiratory syncytial virus (rsv) was compared with two live vaccines. the inactivated (gc) vaccine consisted of glutaraldehyde-fixed bovine nasal mucosa cells persistently infected with rsv and emulsified with oil adjuvant. the live vaccines were a modified virus (mv) derived from a bovine strain of rsv and a temperature-sensitive mutant (ts-1) derived from a human strain. the gc vaccine was inoculated subcutaneously into 12 calves and the live vaccines intramuscu ... | 1984 | 6501875 |
| cellular immune response to infection with respiratory syncytial virus and influence of breast-feeding on the response. | virus-specific lymphocyte transformation (ltf) activity in vitro was examined in 78 infants with various forms of illness due to respiratory syncytial virus (rsv) infection. in 73 subjects with lower respiratory tract disease, significant ltf activity was often detected within one week after onset of initial symptoms, and responses characteristic for each clinical form were observed in the subsequent rise. thus, mean activity in subjects with tracheobronchitis increased gradually, with the maxim ... | 1984 | 6512509 |
| prevalence of antibodies to respiratory syncytial virus in various populations in israel. | a preliminary study on the prevalence of antibodies to respiratory syncytial virus (rsv) in individuals of various age-groups was carried out. in healthy infants aged 6 to 12 months, the prevalence was 27%. it increased to 37 and 54% in children aged 1 to 2 and 2 to 4 years, respectively. the prevalence reached 78% in healthy individuals who were more than 20 years old, including pregnant women. significant levels of specific antibodies were detected in 59% of children aged 6 to 12 months who we ... | 1984 | 6519945 |
| outbreak of respiratory syncytial virus infection in a neonatal intensive care unit. | 1984 | 6519955 | |
| interaction of polymorphonuclear leukocytes and viruses in humans: adherence of polymorphonuclear leukocytes to respiratory syncytial virus-infected cells. | the nature of neutrophil-respiratory syncytial virus (rsv) interaction was investigated by assessing factors that influence neutrophil adherence to rsv-infected tissue culture monolayers. the adherence of neutrophils to infected cells was directly proportional to the degree of rsv replication as evidenced by infectious virus production, cytopathological changes, or viral antigen appearance. sixty-one percent of the neutrophils adhered to the rsv-infected cells as compared with 52.7% on noninfect ... | 1984 | 6481852 |
| transcriptional mapping of human respiratory syncytial virus. | a transcriptional map for human respiratory syncytial virus was determined by measuring the kinetics of viral gene inactivation in response to uv irradiation. monolayer cell cultures of respiratory syncytial virus-infected hep-2 cells were exposed to uv light, and residual viral rna synthesis was monitored both by gel electrophoresis and by hybridization to dot blots of cloned cdnas of the 10 known viral genes. target sizes for the 10 individual viral genes were calculated relative to the uv sen ... | 1984 | 6492254 |
| field trials of a bovine respiratory syncytial virus vaccine. | a vaccine against bovine respiratory syncytial virus (brsv: norden), a cause of pulmonary edema-emphysema and pneumonia in calves, was evaluated in field trials on western nebraska beef calves at branding, preweaning, weaning and postweaning. of calves with undifferentiated respiratory infection at weaning, 20.9% of 2-dose vaccinates (169/810) and 27.5% of 1-dose vaccinates (230/837) required treatment, vs 48.2% of unvaccinated controls (397/823). at postweaning, 2.4% of 2-dose vaccinates (13/54 ... | 1984 | 6493205 |
| an outbreak of respiratory syncytial virus pneumonia in a nursing home for the elderly. | an outbreak of pneumonia and febrile respiratory illness took place in a los angeles county nursing home for the elderly in february and march of 1979. forty of 101 (40%) residents were affected. twenty-two (55%) had pneumonia and eight (20%) died. the outbreak extended over a period of 6 weeks. serological evidence implicated respiratory syncytial virus (rsv) as the causative agent. three patients had fourfold or greater rises in the titre of antibody to rsv, one had high titres in paired sampl ... | 1984 | 6527041 |
| the development of respiratory syncytial virus-specific immune complexes in nasopharyngeal secretions following natural infection. | sequential samples of nasopharyngeal secretions (nps) from 61 infants and children with respiratory syncytial virus (rsv) infection were examined for the presence of rsv-specific immune complexes in order to determine their possible role in recovery from or pathogenesis of rsv infection. immune complexes in nps were identified by the raji cell assay using indirect immunofluorescence and rsv antigen in these complexes was also detected by indirect immunofluorescence. rsv-specific immune complexes ... | 1984 | 6527374 |
| a preliminary serological survey of viral antibodies in peruvian sheep. | this study reports the sero-prevalence of viral infections in sheep in peru. serum samples were collected from 34 mature healthy rams located in 3 different geographic regions of the country (north, central and south). the sera were tested for antibodies to the following viruses: respiratory syncytial virus (rsv); parainfluenza 3 (pi-3) virus; bovine viral diarrhea/border disease (bvd/bd) virus; bovine herpesvirus 1 (bhv-1); bluetongue (bt) virus; ovine progressive pneumoniae (opp) virus; bovine ... | 1984 | 6528458 |
| [demonstration of respiratory syncytial virus infection in calves by the complement fixation reaction]. | studied was tentatively the occurrence of a respiratory syncytial virus infection on some farms with records of the disease. investigations were carried out on the base of the complement-fixation test (cft) specially worked out and on the specific complement-fixing antigen obtained from a bovine respiratory syncytial virus (brsv). tested were several methods for the production of a complement-fixing antigen. most appropriate proved the one obtained by the extraction of an infected cell monolayer ... | 1984 | 6528480 |
| propagation of bovine respiratory syncytial virus in calf testicle microcarrier culture. | 1984 | 6543300 | |
| identification of a tenth mrna of respiratory syncytial virus and assignment of polypeptides to the 10 viral genes. | nine mrnas, their cdna clones, and a genome transcriptional map have been reported previously for respiratory syncytial virus (p. l. collins and g. w. wertz, proc. natl. acad. sci. u.s.a. 80:3208-3212, 1983). we report here the identification of a 10th viral mrna, designated mrna 2b (molecular weight [mw] ca. 0.39 x 10(6)), that was detected by rna (northern) blot hybridization with cdna clones. analysis of a polycistronic readthrough transcript was used to deduce the position in the viral trans ... | 1984 | 6546401 |
| sequence analysis of the respiratory syncytial virus phosphoprotein gene. | a recombinant cdna plasmid (prsa3) containing an almost full-length copy of the mrna encoding respiratory syncytial virus phosphoprotein was identified in a cdna library prepared with mrna from respiratory syncytial virus-infected cells. the cdna insert was sequenced, and a protein of 27,150 daltons was deduced from the dna sequence. the protein is relatively acidic, containing two clusters of acidic amino acids, one in the middle of the molecule and the other at the c-terminus. it is devoid of ... | 1984 | 6548527 |
| immune adherence hemagglutination test for detection of antibody to respiratory syncytial virus. brief report. | an immune adherence hemagglutination test was established for detection of antibodies to respiratory syncytial virus. the test was shown to be as specific as and much more sensitive than the neutralization and complement fixation tests. | 1984 | 6497660 |
| [viral-microbial associations and the function of humoral factors of natural immunity in acute pneumonia patients]. | a total of 359 patients with acute pneumonia and 152 practically healthy subjects comprising the control group were examined. immunofluorescence was used to investigate nasopharyngeal washings for detecting antigens of influenza and parainfluenza viruses, respiratory-syncytial virus, adenoviruses, whereas serological studies according to the hemagglutination delay test with diagnostic agents for detecting influenza a1, a2, b, types 1, 2 and 3 parainfluenza, and the complement fixation test were ... | 1984 | 6523359 |
| broad-spectrum antiviral activity of adenosine analogues. | in recent years certain aliphatic and carbocyclic adenosine analogues have been developed which are of potential clinical importance as antiviral agents. this includes (s)-9-(2,3-dihydroxypropyl)adenine [(s)-dhpa] and carbocyclic 3-deazaadenosine (c-c3ado). (s)-dhpa and c-c3ado are remarkably similar in their antiviral spectrum in that they are particularly active against (-) rna viruses such as measles, para-influenza, respiratory syncytial virus, rabies virus, vesicular stomatitis virus and (+ ... | 1984 | 6476818 |
| use of the biotin-avidin system to study the specificity of antibodies against respiratory syncytial virus. | to characterize the specificity of monoclonal antibodies against respiratory syncytial virus, we developed a technique which combined the biotin-avidin system, immunoprecipitation, and transblot electrophoresis. the viral proteins were first biotinylated and then immunoprecipitated with a monoclonal antibody or respiratory syncytial virus immune serum, separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and electrophoretically transferred to nitrocellulose paper. the protein ... | 1984 | 6470104 |
| suppression of respiratory syncytial virus infection in cotton rats by bis(5-amidino-2-benzimidazolyl)methane. | intraperitoneal administration of bis(5-amidino-2-benzimidazolyl)methane at well-tolerated daily doses of 25 mg/kg subsequent to challenge and for 3 days thereafter effected over a 1-log reduction in the amount of virus recovered from lungs of cotton rats inoculated intratracheally with respiratory syncytial virus. when animals were immunosuppressed to prolong virus shedding, the reduction in recovered virus achieved with a 7-day dosing schedule of bis(5-amidino-2-benzimidazolyl)methane exceeded ... | 1984 | 6393863 |
| respiratory syncytial virus-related apnea in infants. demographics and outcome. | medical records of 261 hospitalized patients less than 1 year old with documented respiratory syncytial virus (rsv) infection were reviewed to determine the incidence of rsv-associated apnea and the accompanying risk of subsequent apnea or death. apnea in association with rsv infection occurred in 18% of the infants. premature birth and a young postnatal age were risk factors for development of apnea with rsv disease. apnea of prematurity appeared to be a significant risk factor for rsv apnea de ... | 1984 | 6702769 |