Publications
Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
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wheezing, asthma, and pulmonary dysfunction 10 years after infection with respiratory syncytial virus in infancy. | of the 180 children admitted to hospitals in tyneside in the first year of life with proved respiratory syncytial virus lower respiratory tract infection, 130 were seen for review 10 years later and 34 of the remaining 50 children accounted for. skin tests, lung function tests, and histamine-challenge and exercise tests for bronchial lability were undertaken in over 100 of the index children and a similar number of control children. a total of 55 (42%) of the 130 index children had had further e ... | 1982 | 6805648 |
enzyme-linked immunosorbent assay for detection of respiratory syncytial virus infection: application to clinical samples. | an enzyme-linked immunosorbent assay (elisa) for respiratory syncytial virus antigens was applied to the rapid diagnosis of acute infections in children and was compared with viral culture and immunofluorescence tests. the elisa test employed commercially available reagents and was run on a day-to-day basis as specimens were received in the laboratory. sensitivity and specificity by elisa were 82 and 95%, respectively, compared with culture. in the same specimens, the sensitivity and specificity ... | 1982 | 6749895 |
comparison of virus culturing and immunofluorescence for rapid detection of respiratory syncytial virus in nasopharyngeal secretions: sensitivity and specificity. | i compared the fluorescent antibody test for respiratory syncytial virus in nasal secretions to virus cultures (n = 310). the sensitivity, specificity, and predictive value (+) were 95.1%, 86.5%, and 88.5%, respectively. | 1982 | 6749901 |
parenteral immunization with live respiratory syncytial virus is blocked in seropositive cotton rats. | seropositive cotton rats cannot be immunized by intramuscular inoculation of live respiratory syncytial virus. passively administered antiserum was shown to effect the immunosuppression. by contrast, the presence of serum antibody did not block immunization by the intranasal route. | 1982 | 6752019 |
clinical and pathological observations on spontaneous bovine respiratory syncytial virus infections in calves. | a clinical, pathological and microbiological study was made of acute spontaneous bovine respiratory syncytial virus infection in calves. characteristic features were atelectatic areas, which had an exudative and, or, necrotising bronchiolitis with syncytial giant cells in the epithelial lining and in the lumina of these bronchioles. restricted to these areas both bronchiolar and alveolar immunofluorescence for bovine respiratory syncytial virus were seen. complications were severe interstitial o ... | 1982 | 6755591 |
respiratory syncytial virus: its transmission in the hospital environment. | respiratory syncytial virus (rsv) over the past two decades has been recognized as the most important cause of lower respiratory tract disease in infants and young children. recently, it has also been identified as a major nosocomial hazard on pediatric wards. the potential for rsv to spread on such wards is underlined by several singular characteristics of rsv. it arrives in yearly epidemics and is highly contagious in all age groups. immunity is of short duration, allowing repeated infections ... | 1982 | 6758370 |
atopy predisposing to acute bronchiolitis during an epidemic of respiratory syncytial virus. | thirty-one infants admitted to hospital with acute bronchiolitis during an epidemic of respiratory syncytial virus were compared with a control group of 32 infants to establish whether the two groups differed in atopic background. past history of respiratory illness, eczema, and present reactions to skin testing differed significantly between the two groups. thus, infants with acute bronchiolitis had a significantly higher atopic predisposition than the controls. | 1982 | 6802409 |
outcome of acute lower respiratory tract infection in infants: preliminary report of seven-year follow-up study. | seven years after admission to hospital for acute lower respiratory tract infection in infancy 200 children and their matched controls were assessed for respiratory state and epidemiological characteristics. the index group comprised 100 cases where respiratory syncytial virus infection had been responsible for the index illness (group 1) and 100 cases in whom this organism had not been found (group 2). there were no differences between index and control groups in birth weight or gestational age ... | 1982 | 6807471 |
an indirect haemadsorption procedure (mrspah) for detecting antibodies to respiratory syncytial virus. | a simple haemadsorption procedure for the detection of isotype specific antibodies to r.s. virus has been developed. correlation between detection of r.s. virus igg antibodies by mixed reverse (solid phase) passive antiglobulin haemadsorption (mrspah) and other serological procedures has been obtained. iga antibody was detected in 62% of milk samples studied. the method is rapid, simple and uses commonly available serological reagents. | 1982 | 6759520 |
in vivo inhibition of respiratory syncytial virus by ribavirin. | ribavirin reduced the amount of respiratory syncytial virus in nasal turbinates and lung tissues of experimentally infected cotton rats by over 90%. an effect was seen when the drug was given either intraperitoneally or by aerosol; however, the antiviral effect was achieved at much lower doses when delivered by the aerosol route. no animal deaths due to the drug were seen. | 1982 | 7044296 |
experimental infection of lambs with bovine respiratory syncytial virus and pasteurella haemolytica: immunofluorescent and electron microscopic studies. | colostrum-deprived lambs were inoculated transtracheally with respiratory syncytial virus (rsv), pasteurella haemolytica, or rsv and p haemolytica. multiple tissues were examined by immunofluorescence to localize viral and bacterial antigens, and lungs were examined by electron microscopy for cytopathologic changes. using immunofluorescence, viral antigen was detected only in the respiratory tract, mainly in the bronchial and bronchiolar epithelium and in the alveolar wall. lesser amounts of vir ... | 1982 | 7046531 |
comparison of direct and indirect immunofluorescence staining of clinical specimens for detection of respiratory syncytial virus antigen. | immunofluorescence staining methods for respiratory syncytial virus antigen detection were compared. of 50 specimens originally positive for respiratory syncytial virus by direct immunofluorescence and culture, 49 were positive by repeat direct immunofluorescence and 32 were positive by indirect immunofluorescence. additional results obtained on specimens originally negative for respiratory syncytial virus by direct immunofluorescence, culture, or both indicate that direct immunofluorescence sta ... | 1982 | 7047562 |
atypical neonatal respiratory syncytial virus infection. | 1982 | 7082462 | |
respiratory syncytial virus pneumonia. | 1982 | 7062498 | |
analysis of proteins synthesized in respiratory syncytial virus-infected cells. | the spectrum of respiratory syncytial virus-encoded proteins was examined in infected cell extracts by standard polyacrylamide gel electrophoresis and by two-dimensional gel analysis. polyacrylamide gel electrophoresis analysis of a variety of respiratory syncytial virus-infected, actinomycin d-treated cell lines revealed the presence of as many as nine virus-encoded proteins. seven of these nine proteins were immunoprecipitated by anti-respiratory syncytial serum. only one major band of [3h]glu ... | 1982 | 7086963 |
respiratory syncytial viral infection in infants with congenital heart disease. | occasional reports have suggested that infants with congenital heart disease may have an increased risk of severe illness from respiratory syncytial virus (rsv) infection. we prospectively studied 699 infants hospitalized during the winters of 1976 through 1980, when rsv was prevalent in the community; 229 of these infants had proved rsv infections acquired either before admission or during hospitalization; 27 had both congenital heart disease and rsv infection, and 46 had congenital heart disea ... | 1982 | 7088112 |
serological evidence for the intervention of bovine respiratory syncytial virus in a respiratory disease outbreak in moroccan cattle. | 1982 | 7090648 | |
enzyme-linked immunosorbent assay for detection of respiratory syncytial virus infection: development and description. | an indirect enzyme-linked immunosorbent assay for detection of respiratory syncytial virus (rsv) antigens was developed, using commercially available antisera. horse anti-rsv and calf antiserum to bovine rsv were used as capture and detector antibodies, respectively. the assay could detect as few as 50 pfu of unpurified rsv per ml in infected cell culture supernatant fluids and as little as 10 ng of affinity-purified rsv antigen per ml. no cross-reactions were observed with heterologous virus ty ... | 1982 | 6749894 |
radioimmunoassay of measles virus hemagglutinin protein g. | guinea pig and rabbit antisera from animals immunized with purified measles virus hemagglutinin (g) protein were used to establish a solid-phase four-layer radioimmunoassay for quantitative measurement of the g protein. the sensitive of the assay was 2 ng of purified g protein, and 200 micrograms of protein from uninfected vero cells neither decreased the sensitivity nor reacted non-specifically in the assay. radioimmunoassay standard dose-response curves were established and unknown values inte ... | 1982 | 7130334 |
antiviral activity of sodium 5-aminosulfonyl-2,4-dichlorobenzoate (m12325). | sodium 5-aminosulfonyl-2,4-dichlorobenzoate (m12325) was evaluated for antiviral activity in tissue culture and infected mice. at concentrations ranging from 2.5 to 75.8 micrograms/ml, m12325 inhibited the cytopathic effects of 10 mean tissue culture infective doses of influenza virus a/wsn, a/fm, a/kumamoto, and b/great lakes; parainfluenza virus; rhinovirus; echovirus; respiratory syncytial virus; and vesicular stomatitis virus. concentrations up to 150 micrograms/ml did not inhibit the cytopa ... | 1982 | 6927286 |
respiratory syncytial virus infections in children. | 1982 | 7183846 | |
consideration regarding infection during hospital employment. | among the most important responsibilities of a hospital's employees' health service is the prevention or prompt identification of infectious illnesses among staff members. infections constitute a major health hazard of hospital employment and, both intrinsically or as a consequence of subsequent transmission to hospitalized patients, can have devastating consequences. the risks associated with tuberculosis, viral hepatitis, rubella, herpes, meningococcal disease, and scabies are generally acknow ... | 1982 | 7054483 |
antibody content of rabbit milk and serum following inhalation or ingestion of respiratory syncytial virus and bovine serum albumin. | 1982 | 7200842 | |
respiratory syncytial virus and otitis media with effusion. | 1982 | 7201016 | |
experimental pneumovirus infections. 2. hydrocephalus of hamsters and mice due to infection with human respiratory syncytial virus (rs). | respiratory syncytial virus (rs) injected into suckling hamsters and mice via the cerebral route induced hydrocephalus in 35% of the animals. the pathogenesis of the condition has been examined by light microscopy, immunofluorescence, and virus isolation. infection of leptomeninges and ependyma was revealed by immunofluorescence of virus-specific antigens between days 3 and 7 after inoculation. histologically, eosinophilic inclusion bodies were visible within the cytoplasma of ependymal and meni ... | 1982 | 7065124 |
appearance of complement components and immunoglobulins on nasopharyngeal epithelial cells following naturally acquired infection with respiratory syncytial virus. | nasopharyngeal epithelial cells (npec) were collected from 144 infants and children with respiratory syncytial virus (rsv) infection, and were analyzed by fluorescent antibody techniques for the presence of cell-bound complement (c'3), iga, igg, and igm class of immunoglobulins (ig), and respiratory syncytial virus antigen. viral antigen was present on the surface of npec in 100% of samples obtained in the first 3 days or illness. the percentage of patients positive for rsv antigen declined stea ... | 1982 | 7069409 |
the association of apnea and respiratory syncytial virus infection in infants. | 1982 | 7086623 | |
experimental infection of lambs with bovine respiratory syncytial virus and pasteurella haemolytica: pathologic studies. | nineteen 4-week-old, colostrum-deprived lambs were transtracheally inoculated with respiratory syncytial virus (rsv), pasteurella haemolytica biotype a serotype 1, or rsv and p haemolytica. pneumonic lesions were more frequent, more extensive, and more severe in lambs inoculated with rsv and p haemolytica than in lambs inoculated with either agent alone. lesions were seen in 2 of 4 lambs inoculated with p haemolytica alone, in 3 of 4 lambs inoculated with rsv alone, and in 11 of 11 lambs inocula ... | 1982 | 7091824 |
experimental infection of lambs with bovine respiratory syncytial virus and pasteurella haemolytica: clinical and microbiologic studies. | four-week-old lambs were inoculated transtracheally with respiratory syncytial virus (rsv), pasteurella haemolytica, or rsv and p haemolytica. when given in combination, rsv administration preceded p haemolytica by 3 or 5 days. lambs inoculated with p haemolytica or rsv developed a mild respiratory tract disease accompanied by a transient pyrexia in a few lambs. by 24 hours after inoculation of bacteria, all lambs inoculated with rsv and p haemolytica were listless, reluctant to move, and exhibi ... | 1982 | 7091825 |
experimental viral arthritis. | an early, acute arthritis was observed in 8 of 22 mice experimentally infected with respiratory syncytial virus. no abnormalities were found in the joints of the 12 control mice. the pathological changes in joints of the infected animals included pannus formation and erosion, resembling changes that occur in arthritis in man, including rheumatoid arthritis. it is proposed, therefore, that viruses may play an aetiological role in acute and chronic arthritis in man. these investigations provide a ... | 1982 | 7093144 |
apnoea and bronchiolitis due to respiratory syncytial virus. | 1982 | 7103880 | |
a natural outbreak of bovine respiratory disease caused by bovine respiratory syncytial virus. | an outbreak of respiratory disease occurred in a herd of 34 calves and 5 cows in a barn. the disease which affected 24 calves and 1 cow, was characterized by coughing, fever, anorexia, nasal discharge and pulmonary rales. two calves died 5 days after the disease outbreak and were necropsied. bronchitis, bronchiolitis and alveolitis were among the lesions observed in these calves. bovine respiratory syncytial virus was detected, in lung sections of the dead calves and also in nasal washings of so ... | 1982 | 7105764 |
comparison of enzyme-linked immunosorbent assay and neutralization techniques for measurement of antibody to respiratory syncytial virus: implications for parenteral immunization with live virus vaccine. | the sensitivity of an enzyme-linked immunosorbent assay (elisa) to detect low levels of antibody to respiratory syncytial (rs) virus was compared with a tube dilution neutralization test (neut) on sera obtained from children who received a parenteral live rs virus vaccine. among the children who developed antibody in response to live rs virus vaccine. elisa was as sensitive as neut at detecting antibody increases. some children who did not have detectable prevaccine elisa antibody possessed neut ... | 1982 | 7107002 |
immunochemical identification of viral and nonviral proteins of the respiratory syncytial virus virion. | we identified by immunochemical methods 13 polypeptides associated with the infectious respiratory syncytial virus virion. eight of these polypeptides (vp200, vp84, vp66, vp43, vp40, vp37, vp28, and vp19) were identified as virus specific. two other polypeptides, (vp) 22 and (vp) 12, are provisionally considered to be of viral origin. three nonviral proteins are also intimately associated with the infectious virion. these nonviral proteins were identified as cellular actin and two proteins with ... | 1982 | 7107006 |
administration of a highly attenuated, live respiratory syncytial virus vaccine to adults and children. | a highly attenuated respiratory syncytial virus (rsv) experimental vaccine, rsv ts-2, was sequentially evaluated in adults, seropositive children, and finally, fully susceptible seronegative children. the vaccine was administered intranasally in doses ranging from 10(5.2) to 10(6.3) pfu/ml. in both adults and children, the vaccine proved to be poorly infectious. although poor infectivity would not have been predicted from tissue culture studies of rsv ts-2 growth, the human experience closely pa ... | 1982 | 7107009 |
parenteral administration of live respiratory syncytial virus vaccine: results of a field trial. | a live respiratory syncytial virus (rsv) vaccine administered parenterally was evaluated for efficacy in a double-blind, placebo-controlled field trial in 510 children six to 47 months of age. among the 233 recipients of live rsv vaccine, 68 of 98 initially seronegative children (as determined by enzyme-linked immunosorbent assay) developed antibody to rsv; 25 of the 30 initially seronegative children who did not develop antibody were less than 12 months old, which suggested that very low levels ... | 1982 | 7037983 |
[detection of antibodies to respiratory syncytial virus in a neonate with multiple abnormalities]. | 1982 | 7125129 | |
antibody against viruses in maternal and cord sera: specific antibody is concentrated on the fetal side of the circulation. | paired maternal and cord sera from 100 pregnancies were tested for antibodies against herpes simplex virus, measles virus and respiratory syncytial virus by complement fixation and for antibodies against rubella virus, influenza a virus and influenza b virus by haemagglutination-inhibition. for four viruses (herpes simplex, measles, respiratory syncytial and rubella) higher levels of antibody were found in cord than in maternal sera. there was no difference between maternal and cord serum titres ... | 1982 | 7130705 |
detection of respiratory syncytial virus in nasopharyngeal secretions by elisa: comparison with fluorescent antibody technique. | samples of nasopharyngeal secretions (nps) obtained from 140 infants and children with acute respiratory disease were examined for the presence of respiratory syncytial (rs) virus by elisa. an antiserum produced in rabbits against rs-virus polypeptides was used both as the "capture" antibody and as conjugate in a double-antibody sandwich technique. rs virus was detected by elisa in specimens of nps obtained from 72 (51%) of the patients. by comparison rs virus was shown to be present in 86 (61%) ... | 1982 | 7161598 |
development of antibody-dependent cell-mediated cytotoxicity in the respiratory tract after natural infection with respiratory syncytial virus. | antibody-dependent cell-mediated cytotoxicity (adcc) was measured in nasopharyngeal secretions collected from 42 infants and young children at various intervals after primary or secondary infection with respiratory syncytial virus. adcc was determined by specific immune release of 51cr from respiratory syncytial virus-infected hep-2 cell culture monolayers, with lymphocytes from adult volunteers as effector cells. specific adcc responses in nasopharyngeal secretions after primary infection were ... | 1982 | 7118247 |
virus-cell interactions: nuclear magnetic resonance behaviour of intracellular water. | the water proton nmr spin-lattice relaxation time if measured in hep-2 cells in relation to the infection by different viruses (poliovirus type 1, coxsackievirus b-3, human adenovirus, measles virus, respiratory syncytial virus, herpes simplex virus, subtype 1, and vaccinia virus). the nmr properties of intracellular water allow the early detection and identification of viruses. a close relationship is shown between the nmr behavior and the mode of virus-cell interaction. | 1982 | 7121298 |
respiratory syncytial virus infection in cyclophosphamide-treated cotton rats. | cotton rats infected intranasally with respiratory syncytial virus and immunosuppressed with cyclophosphamide shed virus for at least 7 weeks. dissemination of virus beyond the respiratory tract was observed. in contrast, virus was recovered from infected, non-immunosuppressed rats for only 1 week, and only from the respiratory tract. | 1982 | 7049952 |
clinical evaluation of radioimmunoassay of nasopharyngeal secretions and serology for diagnosis of viral infections in children hospitalized for respiratory infections. | viral diagnosis was performed using radioimmunoassay (ria) for virus antigen in nasopharyngeal secretions (nps) and complement-fixation (cf) tests of paired sera from specimens of 90 children hospitalized for acute respiratory infection. major respiratory viruses sought for by both methods (adenoviruses, influenza a and b viruses, parainfluenza virus type 3, respiratory syncytial virus) were detected in 40 (44%) of the patients; 15% of the diagnoses were made by nps-ria alone. serologic diagnosi ... | 1982 | 7163028 |
lack of relationship of community patterns of respiratory viral activity to systemic haemophilus influenzae disease in children. | the occurrence of epidemics of respiratory viral illness was monitored by virus isolations in a population of young children living in the greater nashville area over a 4-year period. epidemic disease due to influenza, respiratory syncytial virus and parainfluenza type 1 was present during discrete time blocks over this interval. occurrence of systemic haemophilus influenzae type b disease in the community was also documented by review of bacteriologic records of vanderbilt children's hospital o ... | 1982 | 7163030 |
[encephalopathy producing antibodies against respiratory syncytial virus in the central nervous system in an adult woman]. | 1982 | 7164044 | |
fatal haemorrhagic pneumonia in an adult due to respiratory syncytial virus and staphylococcus aureus. | 1982 | 7185980 | |
antibodies to respiratory syncytial virus polypeptides and their significance in human infection. | the human antibody response to respiratory syncytial (rs) virus infection was investigated using radioimmunoprecipitation analysis (ripa). a total of nine rs virus-specific proteins, vp200, vgp95, vp68, vgp48, vpn41, vp35, vp27, vp23 and vgp20 were identified by comparing 35s- or 3h-labelled extracts of infected and uninfected hep-2 cells, and by radioimmunoprecipitation using a hyperimmune human serum. three glycopeptides, vgp95, vgp48 and vgp20, were identified by incorporation of [3h]glucosam ... | 1983 | 6886679 |
replication of respiratory syncytial virus in lungs of immunodeficient mice. | respiratory syncytial virus was frequently isolated during a 10-day test period from the lungs of 4- to 6-week-old immunodeficient nude (nu/nu) mice and from gamma-irradiated c3h mice inoculated intranasally with this virus, but not from similar aged and comparably inoculated normal littermates of these mice. virus isolation rates and levels of virus in lungs in both groups of immunodeficient mice were similar. no extrapulmonary dissemination of virus was observed in any test group of mice. | 1983 | 6887156 |
viral and bacterial pathogens of suspected sepsis in young infants. | clinical and laboratory data of 27 previously normal, healthy infants 2 months of age or less who were hospitalized for suspected sepsis during a 1-year period were reviewed. results of bacterial cultures and viral studies revealed enteroviruses (seven echoviruses, one coxsackievirus) to be the predominant pathogens identified. other viruses identified were one each of adenovirus, untypeable hemadsorbing virus and respiratory syncytial virus. group b streptococcal and enterococcal bacteremia and ... | 1983 | 6889156 |
[infections in adults and in the elderly caused by respiratory syncytial virus]. | 1983 | 6866148 | |
[isolation of the respiratory syncytial virus in bronchiolitic complications of whooping cough. preliminary research]. | 1983 | 6877195 | |
cell-mediated immune response to respiratory syncytial virus infection in owl monkeys. | the suitability of owl monkeys as experimental models to study the cellular immune response to respiratory syncytial virus (rsv) infection was examined. seronegative owl monkeys inoculated intranasally with rsv shed large quantities of virus and developed clinically evident upper respiratory disease. rsv infected monkeys had significant lymphoproliferative responses to rsv antigen by 4 weeks post-infection. in contrast, no positive blastogenic responses were elicited during the acute phase of il ... | 1983 | 6883807 |
a colorimetric assay for quantification of defective interfering particles of respiratory syncytial virus. | a colorimetric assay for defective interfering (di) particles of respiratory syncytial (rs) virus was developed. this quantitative biological assay is based on neutral red dye uptake by di particle-protected cells that survive standard virus challenge. this assay was more sensitive than the reduction of infectious yield (riy) assay and was capable of detecting 1 x 10(4) to 2 x 10(4) di particles/ml. the coefficient of variation for parallel, simultaneous replicates (n = 10) was 23%. cell-protect ... | 1983 | 6854271 |
respiratory syncytial virus infection in young hospitalized children. identification of risk patients and prevention of nosocomial spread by rapid diagnosis. | detection of rsv by immunofluorescence was used for rapid confirmation of the diagnosis in 89 infants during an epidemic. this made it possible to plan the care of the patients and use the personnel in an optimal way in order to maintain nosocomial infection at a low level. somatic and environmental risk factors connected with serious disease were also identified. the course of the disease was more severe in children with chronic illnesses or those who had had perinatal complications. allergy wa ... | 1983 | 6858684 |
presumed respiratory syncytial virus pneumonia in three immunocompromised adults. | three cases of presumed respiratory syncytial virus (rsv) pneumonia in immunocompromised adults are described. two patients had symptoms of cough, fever, and malaise, following completion of a course of combination chemotherapy for the treatment of acute lymphoblastic leukemia. the third patient, a juvenile onset diabetic, developed similar symptoms while hospitalized for severe hyperglycemia. chest roentgenograms showed lower lobe infiltrates in both leukemic patients and a bilateral non-conflu ... | 1983 | 6846393 |
inoculation of hep-2 cells for respiratory syncytial virus isolation. | 1983 | 6848291 | |
respiratory syncytial virus pneumonitis in adults. | two cases of respiratory syncytial virus pneumonitis in adults area described. in both patients, the clinical picture of the adult respiratory distress syndrome, with marked tachypnoea. hypoxaemia and bilateral diffuse pulmonary infiltrates, was present. one patient had systemic lupus erythematosus, while the other had chronic obstructive lung disease and was a heavy drinker of alcohol. both patients survived and recovered after a prolonged stay in hospital. | 1983 | 6835164 |
effect of maternal nutritional status on immunological substances in human colostrum and milk. | substances in colostrum and breast milk confer significant disease resistance to the breast-fed infant. the influence of maternal nutritional status on both immunological and nonimmunological milk factors was studied in a group of 23 colombian women during the first 2 months of lactation. maternal malnutrition was characterized by significantly lower weight/height ratio, creatinine/height index, total serum proteins, serum albumin, and serum igg and iga. the colostrum of malnourished mothers con ... | 1983 | 6837495 |
isolation of respiratory syncytial and influenza viruses from the sputum of patients hospitalized with pneumonia. | routinely collected sputum specimens from 100 adults hospitalized with pneumonia were frozen at -70 c until inoculation into madin-darby canine kidney, fetal tonsil, and esophageal epithelial cells. six influenza a (h3n2) viruses, two respiratory syncytial viruses, three rhinoviruses, and nine herpes simplex viruses were recovered. four patients with influenza virus and one with respiratory syncytial virus isolated had nosocomial pneumonia. viral isolation from sputum specimens may aid the diagn ... | 1983 | 6827134 |
respiratory syncytial virus polypeptides. iii. the envelope-associated proteins. | four proteins, gp1, vgp48, gp26 and vpm27, are associated with the envelope of respiratory syncytial (rs) virus. the status of gp1 has been uncertain, because a cellular glycoprotein migrates at the same position when laemmli's discontinuous buffer system is used for page, and because bsc-1 cells infected with the rsn-2 strain of rs virus appear not to contain gp1. however, additional evidence suggests that gp1 is a viral structural protein. (i) it is removed from cells by trypsin, while the cel ... | 1983 | 6834007 |
identification of the virus-specific proteins of respiratory syncytial virus temperature-sensitive mutants by immunoprecipitation. | the proteins of long strain rsv and three temperature-sensitive (ts) mutants of the a2 strain were compared by pulse labeling virus-infected cells with [35s]methionine and [3h]glucosamine followed by analysis of the cell lysates by polyacrylamide gel electrophoresis. at the permissive temperature (30 degrees) proteins ranging in molecular weight from 24,000 to 50,000 (vp24, vp27, vp33, vp44) could be identified. immunoprecipitation of viral lysates by immune rabbit serum demonstrated antigenic s ... | 1983 | 6828465 |
[respiratory syncytial virus in bovine practice]. | 1983 | 6857220 | |
cellular and humoral immunity in recurrent respiratory syncytial virus infections. | 1983 | 6622111 | |
total serum ige levels in venezuelan schoolchildren. | seventy-eight disease-free children were evaluated by prist for total serum ige in order to establish the normal range for this immunoglobulin and assess its utility, in tropical climates, such as venezuela, as a diagnostic tool for clinical allergy. seventy-eight normals were selected from a group of 1053 children, aged 7-12 years from fourteen schools in caracas. exclusion from the normal pool was based on nationality, and on historical, clinical, and/or laboratory evidence of atopic and/or in ... | 1983 | 6640887 |
[hospital epidemic outbreak of respiratory syncytial virus]. | 1983 | 6676858 | |
respiratory syncytial virus. | 1983 | 6662671 | |
respiratory syncytial virus mrna coding assignments. | the polypeptide coding assignments for six of the respiratory syncytial virus-specific mrnas were determined by translation of the individual mrnas in vitro. the coding assignments of the rnas are as follows. rna band 1 is complex and can be separated into at least two components on the basis of electrophoretic mobility (molecular weights [mws] approximately equal to 0.21 x 10(6) and 0.31 x 10(6), respectively) that code for three polypeptides of 9.5, 11, and 14 kilodaltons (k). rna 2 (mw, 0.39 ... | 1983 | 6682456 |
serology for diagnosis and epizootiological studies of bovine respiratory syncytial virus infections. | the complement fixation test (cft) and the virus neutralisation test (vnt), performed as a plaque reduction test, were employed to measure antibodies to bovine respiratory syncytial virus. the cft with bovine sera was performed with supplementation of the complement factors in fresh guinea pig serum by an adequate amount of clq-factor of the bovine species. kinetics of maternally derived antibodies and the antibody response after spontaneous and experimental infections and after intramuscular va ... | 1983 | 6635341 |
aromatic amidines: comparison of their ability to block respiratory syncytial virus induced cell fusion and to inhibit plasmin, urokinase, thrombin, and trypsin. | two series of amidine derivatives consisting of a total of 24 compounds were examined for a correlation between their blocking effect on respiratory syncytial virus induced cell fusion and their inhibitory activity against selected trypsin-like protease. although no correlation was evident between the two activities, several potentially important discoveries were made. a highly selective inhibitor of plasmin over thrombin (compound 10) was obtained, and a potent new blocker of virus-induced cell ... | 1983 | 6219223 |
respiratory syncytial virus infection in the elderly 1976-82. | 1983 | 6416524 | |
hotting-up the complement-fixation test. | a detailed investigation into the effect of modifying the incubation temperature of the complement-fixation (cf) test is described. for varicella-zoster virus cytomegalovirus and rubella virus, increasing the incubation temperature progressively increased the sensitivity of the cf test to reach a maximum at 15 degrees c, at which temperature the geometric mean titre of seropositive samples was significantly greater than that found at 4 degrees c. for these three viruses, each serum shown to cont ... | 1983 | 6296229 |
viral infections in clinical pertussis. | of 44 children with clinical pertussis 29 (66%) had serological evidence of concurrent infection with respiratory viruses or mycoplasma pneumoniae. the commonest superinfection was by mycoplasma (32%), followed by respiratory syncytial virus (27%) and adenoviruses (16%). the high prevalence of viral infections, many being multiple, supports the theory that pertussis predisposes to such infections or vice versa. | 1983 | 6623245 |
[asthmatic syndrome in respiratory syncytial virus infection associated with influenza in young children]. | 1983 | 6356010 | |
respiratory syncytial virus pneumonia in young calves: clinical and pathologic findings. | nine young calves given respiratory syncytial virus by a combined intranasal and intratracheal route developed a severe respiratory tract disease in which coughing, tachypnea, and hyperpnea were prominent clinical features. calves were euthanatized on postinoculation (initial) days (pid) 1 to 13. at necropsy, large areas of consolidation were present in the cranial, middle, accessory, and cranial parts of the caudal lung lobes of calves killed between pid 4 and 13. histopathologic examination re ... | 1983 | 6625318 |
respiratory syncytial virus glycoproteins. | the proteins of respiratory syncytial (rs) virus were analyzed by sds-polyacrylamide gel electrophoresis. eight virion structural proteins with molecular weights of 180,000, 89,000, 48,000, 42,000, 34,000, 28,000, 25,000, and 21,000 were identified. these proteins were given tentative designations of l (180,000), g (89,000), f1 (48,000), np (42,000), p (34,000), m (28,000), vp25 (25,000), and f2 (21,000). the 89,000-, 48,000-, and 21,000-dalton polypeptides were glycosylated and could be purifie ... | 1983 | 6636537 |
characterization of the human peripheral blood effector cells mediating antibody-dependent cell-mediated cytotoxicity against respiratory syncytial virus. | peripheral blood lymphocytes were separated into several subpopulations and evaluated for their ability to mediate antibody-dependent cell-mediated cytotoxicity (adcc) against respiratory syncytial virus (rsv)-infected hela cells. using erythrocyte rosetting methods, nylon wool filtration, and cytolysis with okt-3 monoclonal antibody, two lymphocyte subpopulations were shown to mediate rsv-adcc; non-t, non-b, and igg-fc receptor-bearing lymphocytes and e-rosetting cells with iggfc receptors (t g ... | 1983 | 6603313 |
polymorphonuclear function and respiratory syncytial virus infections in children. | 1983 | 6618679 | |
indirect haemagglutination test for the detection and assay of antibody to bovine respiratory syncytial virus. | an indirect haemagglutination (iha) test was used for the rapid assay of antibody to bovine respiratory syncytial virus. antigens for the sensitisation of formalised tanned erythrocytes were prepared by treatment of virus infected cells with non-ionic detergent. a close serological relationship was shown by the iha test between the strain of bovine respiratory syncytial virus used and the a2 strain of human respiratory syncytial virus. the iha test was sensitive and reproducible. a linear correl ... | 1983 | 6636489 |
modified immunoprecipitation procedure for the identification of human respiratory syncytial virus polypeptides. | when analysed by polyacrylamide gel electrophoresis, human respiratory syncytial virus harvested after a one step growth cycle and purified through a continuous sucrose density gradient was shown to be composed of nine structural proteins of 90, 68, 49, 42, 34, 28, 25, 19 an 13 kd. the 90, 49 and 19 kd polypeptides were identified as glycopolypeptides by glucosamine incorporation. a modified immunoprecipitation procedure confirmed the viral specificity of the 49, 42, 28, 25 and 19 kd polypeptide ... | 1983 | 6643642 |
enzyme-linked immunosorbent assay for serum antibody to bovine respiratory syncytial virus: comparison with complement-fixation and neutralization tests. | an indirect enzyme-linked immunosorbent assay (elisa) for the detection of serum antibody to bovine respiratory syncytial virus (brsv) was developed and compared with complement-fixation (cf) and viral neutralization (vn) tests. tissue culture-grown viral antigens were used in the tests. serum samples from various sources were compared, including serum samples from 10 calves which were infected experimentally with brsv by aerosol exposure. the elisa compared favorably with the vn test for detect ... | 1983 | 6660613 |
ribavirin aerosol treatment of bronchiolitis associated with respiratory syncytial virus infection in infants. | in a double-blind study, bronchiolitis associated with respiratory syncytial virus infection in 12 randomly selected patients treated with ribavirin aerosol improved more rapidly than in 14 control patients given saline aerosol (p = .044, wilcoxon rank sum test, two-tailed). an estimated 10 mg of ribavirin per kilogram of body weight was administered in daily 12-hour treatments over a five-day period. respiratory syncytial virus disappeared from secretions at about the same rate in treated and c ... | 1983 | 6356005 |
construction and characterization of cdna clones for four respiratory syncytial viral genes. | cytoplasmic poly(a)-containing rna from respiratory syncytial virus-infected cells was used as a template to synthesize oligo(dt)-primed cdnas. discrete size classes of single-stranded cdnas, resolved by alkali agarose gel electrophoresis, were used separately to construct double-stranded cdnas that were subsequently inserted into the plasmid vector pbr322 at the pst i site by means of oligo(dg)oligo(dc) tailing. after transfection of escherichia coli, recombinant plasmids were screened mostly b ... | 1983 | 6572388 |
mechanisms of immunity to respiratory syncytial virus in cotton rats. | active immunity and maternally transmitted passive immunity to respiratory syncytial virus (rsv) were studied in cotton rats. animals infected with respiratory syncytial virus developed complete resistance to pulmonary reinfection, which lasted at least 18 months. nasal resistance was of shorter duration and began to diminish in 8 months. pulmonary resistance was transferred by parabiosis, but nasal resistance was not. adoptive transfer studies with fractionated convalescent blood showed that se ... | 1983 | 6352505 |
a study of respiratory infections in the elderly to assess the role of respiratory syncytial virus. | a prospective study was performed from 1 december 1981 to 31 may 1982 in two departments of geriatric medicine in edinburgh. this yielded 159 cases of acute respiratory tract infection (rti). twelve of these were undoubtedly associated with respiratory syncytial virus (rsv), 14 with influenza a and 18 with influenza b (as established in each case by a fourfold or greater increase in antibody titre). eighty-five of the 159 patients with rti and rsv titres of 32 or greater. their significance is d ... | 1983 | 6663083 |
[investigation on the level of antibodies against respiratory syncytial virus in children in nanning city]. | 1983 | 6675846 | |
monoclonal antibodies to respiratory syncytial virus proteins: identification of the fusion protein. | six monoclonal antibodies directed against respiratory syncytial virus proteins were produced. each was characterized by immunoprecipitation and indirect immunofluorescence. one was directed against the nucleocapsid protein. np 44, two were directed against a 37,000-dalton protein, two were directed against the major envelope glycoprotein, gp 90, and one was directed against the 70,000-dalton envelope protein, vp 70. indirect immunofluorescence stain patterns of infected hep-2 cells defined gp 9 ... | 1983 | 6345804 |
lung defenses against infection: a clinical correlation. | the current state of knowledge of lung defenses has been reviewed. first, mechanical factors such as aerodynamic filtration and mucociliary transport were considered. then, in general terms, the contributions of alveolar macrophages, neutrophils, lymphocytes, and immunoglobulins, and the roles of complement, antiproteases, lysozyme, and fibronectin were examined. interactions between these components may regulate their effect. finally, the responses to five specific microorganisms were reviewed ... | 1983 | 6346246 |
rapid detection of respiratory syncytial virus with a monoclonal antibody. | we developed an indirect fluorescent-antibody test employing a mouse monoclonal antibody directed against the nucleoprotein of rsv for rapid detection of respiratory syncytial virus (rsv). this test demonstrated distinctive fluorescent inclusions in hep-2 cells infected with 24 rsv isolates collected during 6 previous years but not in cells infected with 13 other respiratory viruses. examination of nasal cells of 100 infants with acute respiratory illness showed that the indirect fluorescent-ant ... | 1983 | 6348077 |
evidence for a viral etiology of paget's disease of bone. | ultrastructural studies of the bone cells in patients with paget's disease of bone have revealed the presence of nuclear and cytoplasmic inclusions that resemble the nucleocapsids of paramyxoviridae virus. immunocytologic studies of bone biopsy specimens and cultured bone cells have demonstrated positive responses with antisera against measles virus and/or respiratory syncytial virus. these observations suggest that paget's disease may be a slow viral infection of bone. the exact nature of the p ... | 1983 | 6349897 |
ribavirin treatment of experimental respiratory syncytial viral infection. a controlled double-blind study in young adults. | the effect of ribavirin aerosol on experimental respiratory syncytial virus (rsv) infection was evaluated in a double-blind controlled study of 16 young adult volunteers. two days after intranasal inoculation with rsv, half of the subjects were treated with a small-particle aerosol of ribavirin and half with placebo for a total of 12 hours each day for three days. seven of eight placebo-treated and six of eight ribavirin-treated subjects became infected. viral shedding was diminished in the riba ... | 1983 | 6341640 |
amino acid sequence of human respiratory syncytial virus nucleocapsid protein. | amino acid sequence of the human respiratory syncytial (rs) virus nucleocapsid (nc) protein, deduced from the dna sequence of a recombinant plasmid, is presented. the cdna plasmid (prsb11) has 1412 bp of rs viral nc sequence and lacks six nucleotides of the 5' end of mrna. there is a single long open reading frame encoding 467 amino acids. this 51540 dal protein is rich in basic amino acids and has no homologies with other known viral capsid proteins. | 1983 | 6310521 |
effect of sucrose phosphate and sorbitol on infectivity of enveloped viruses during storage. | cytomegalovirus and varicella-zoster virus recovery from sucrose phosphate (0.2 m sp) and 70% sorbitol (sorbitol) was compared after storage at -70, 4, and 20 degrees c over time. recovery from 0.2 m sp was uniformly better. more tissue culture infective doses and infectious foci were recovered in cell monolayers inoculated with 0.2 m sp virus stocks as compared with viruses stored in 70% sorbitol. although both viruses were isolated from diluted fresh stocks (10(-1) through 10(-4], freezing dil ... | 1983 | 6313747 |
viral infections of the respiratory tract in hospitalized children. a study from oslo during a 90 months' period. | a diagnosis of 979 respiratory viral infections was made in hospitalized children. respiratory syncytial virus greatly out-numbered the other viruses: it caused 58% of the total virus infections and occurred in winter epidemics. influenza a and b virus occurred during late winter and spring, rhinovirus had a seasonal distribution towards spring and autumn, whereas adenovirus types 1, 2 and 5 had no distinct seasonal distribution. whereas respiratory syncytial virus were mainly associated with br ... | 1983 | 6305106 |
report of a workshop on respiratory syncytial virus and parainfluenza viruses. | 1983 | 6194232 | |
respiratory syncytial virus detection by immunofluorescence in nasal secretions with monoclonal antibodies against selected surface and internal proteins. | specimens containing respiratory tract epithelial cells from infants and children with acute respiratory disease were evaluated by using an indirect immunofluorescence technique with two specific respiratory syncytial virus monoclonal antibodies. one (rs/hn 13-1) was directed against a cell surface viral antigen, and the other (rs/hn 25-2) was directed against viral antigen present in large cytoplasmic inclusions. the same results on presence or absence of respiratory syncytial virus were obtain ... | 1983 | 6361054 |
rapid diagnosis of respiratory syncytial virus and influenza a virus infections by immunofluorescence: experience with a simplified procedure for the preparation of cell smears from nasopharyngeal secretions. | a simplified procedure for preparing direct smears of exfoliated cells from nasopharyngeal secretions is presented. the specimens were examined by the indirect immunofluorescence (if) technique for the presence of respiratory syncytial (rs) virus and influenza a virus antigens. specimens from 457 children hospitalised at twelve different hospital departments in norway were examined during the winter season 1980-81. rs virus was identified in samples from 139 children and influenza a virus from 2 ... | 1983 | 6353858 |
experimental respiratory syncytial virus pneumonia in young calves: microbiologic and immunofluorescent findings. | young calves were inoculated with respiratory syncytial virus (rsv) intranasally or by a combined intranasal and intratracheal route and were killed between postinoculation (initial) days (pid) 1 and 14. viral antigens were detected by immunofluorescence in nasopharyngeal cells from calves killed between pid 2 and 10. evidence of infection of the trachea and lungs with rsv was obtained by immunofluorescence and virus isolation in calves inoculated by the combined route, but not in calves inocula ... | 1983 | 6354017 |
correlation of cytotoxic activity in lungs to recovery of normal and gamma-irradiated cotton rats from respiratory syncytial virus infection. | cotton rats (sigmodon hispidus) that were exposed to 300, 600, or 900 rads of gamma irradiation and inoculated intranasally 2 days later with respiratory syncytial virus (rsv) exhibited prolonged virus shedding and delayed humoral and cytotoxic immune responses compared with comparably inoculated nonirradiated control rats. in nonirradiated animals and in animals exposed to 300 and 600 rads, levels of virus declined and then disappeared from the lungs during the period in which cytotoxic activit ... | 1983 | 6354026 |
enzymatic cleavage of a glycoprotein of respiratory syncytial virus. | the 75k glycoprotein of the a2 strain of respiratory syncytial virus was cleaved by digestion with trypsin or staphylococcus aureus protease v8. the fragments resulting from trypsin digestion were 40k and 29k; those from the staphylococcal protease were 49k and 37k. | 1983 | 6354136 |
enhancement of respiratory syncytial virus-induced cytopathology by trypsin, thrombin, and plasmin. | a series of proteases of diverse substrate specificity were tested for their effect on respiratory syncytial virus-induced cytopathology. three of the enzymes, thrombin, plasmin, and trypsin, were able to augment significantly the fusion of virus-infected a549 cells. on a concentration basis, thrombin was the most active promoter, followed by plasmin and then trypsin. hirudin, a specific thrombin inhibitor, blocked the fusion-enhancing property of thrombin, yet had no influence on the basal rate ... | 1983 | 6219957 |