TitleAbstractYear(sorted ascending)
enzyme-linked immunosorbent assay for detection of respiratory syncytial virus infection: development and indirect enzyme-linked immunosorbent assay for detection of respiratory syncytial virus (rsv) antigens was developed, using commercially available antisera. horse anti-rsv and calf antiserum to bovine rsv were used as capture and detector antibodies, respectively. the assay could detect as few as 50 pfu of unpurified rsv per ml in infected cell culture supernatant fluids and as little as 10 ng of affinity-purified rsv antigen per ml. no cross-reactions were observed with heterologous virus ty ...19826749894
comparison of virus culturing and immunofluorescence for rapid detection of respiratory syncytial virus in nasopharyngeal secretions: sensitivity and specificity.i compared the fluorescent antibody test for respiratory syncytial virus in nasal secretions to virus cultures (n = 310). the sensitivity, specificity, and predictive value (+) were 95.1%, 86.5%, and 88.5%, respectively.19826749901
a longitudinal study of respiratory viruses and bacteria in the etiology of acute otitis media with effusion.we analyzed data from a 14-year longitudinal study of respiratory infections in young children to determine the relative importance of viral respiratory infection and nasopharyngeal colonization with streptococcus pneumoniae and haemophilus influenzae as factors influencing the occurrence of acute otitis media with effusion. the incidence of this disorder was increased in children with viral respiratory infections (average relative risk, 3.2; p less than 0.0001). infection with respiratory syncy ...19826281639
parenteral immunization with live respiratory syncytial virus is blocked in seropositive cotton rats.seropositive cotton rats cannot be immunized by intramuscular inoculation of live respiratory syncytial virus. passively administered antiserum was shown to effect the immunosuppression. by contrast, the presence of serum antibody did not block immunization by the intranasal route.19826752019
atopy predisposing to acute bronchiolitis during an epidemic of respiratory syncytial virus.thirty-one infants admitted to hospital with acute bronchiolitis during an epidemic of respiratory syncytial virus were compared with a control group of 32 infants to establish whether the two groups differed in atopic background. past history of respiratory illness, eczema, and present reactions to skin testing differed significantly between the two groups. thus, infants with acute bronchiolitis had a significantly higher atopic predisposition than the controls.19826802409
outcome of acute lower respiratory tract infection in infants: preliminary report of seven-year follow-up years after admission to hospital for acute lower respiratory tract infection in infancy 200 children and their matched controls were assessed for respiratory state and epidemiological characteristics. the index group comprised 100 cases where respiratory syncytial virus infection had been responsible for the index illness (group 1) and 100 cases in whom this organism had not been found (group 2). there were no differences between index and control groups in birth weight or gestational age ...19826807471
wheezing, asthma, and pulmonary dysfunction 10 years after infection with respiratory syncytial virus in infancy. 19826809211
antiviral activity of sodium 5-aminosulfonyl-2,4-dichlorobenzoate (m12325).sodium 5-aminosulfonyl-2,4-dichlorobenzoate (m12325) was evaluated for antiviral activity in tissue culture and infected mice. at concentrations ranging from 2.5 to 75.8 micrograms/ml, m12325 inhibited the cytopathic effects of 10 mean tissue culture infective doses of influenza virus a/wsn, a/fm, a/kumamoto, and b/great lakes; parainfluenza virus; rhinovirus; echovirus; respiratory syncytial virus; and vesicular stomatitis virus. concentrations up to 150 micrograms/ml did not inhibit the cytopa ...19826927286
wheezing, asthma, and pulmonary dysfunction 10 years after infection with respiratory syncytial virus in infancy.of the 180 children admitted to hospitals in tyneside in the first year of life with proved respiratory syncytial virus lower respiratory tract infection, 130 were seen for review 10 years later and 34 of the remaining 50 children accounted for. skin tests, lung function tests, and histamine-challenge and exercise tests for bronchial lability were undertaken in over 100 of the index children and a similar number of control children. a total of 55 (42%) of the 130 index children had had further e ...19826805648
respiratory syncytial virus infection in cyclophosphamide-treated cotton rats.cotton rats infected intranasally with respiratory syncytial virus and immunosuppressed with cyclophosphamide shed virus for at least 7 weeks. dissemination of virus beyond the respiratory tract was observed. in contrast, virus was recovered from infected, non-immunosuppressed rats for only 1 week, and only from the respiratory tract.19827049952
parenteral administration of live respiratory syncytial virus vaccine: results of a field trial.a live respiratory syncytial virus (rsv) vaccine administered parenterally was evaluated for efficacy in a double-blind, placebo-controlled field trial in 510 children six to 47 months of age. among the 233 recipients of live rsv vaccine, 68 of 98 initially seronegative children (as determined by enzyme-linked immunosorbent assay) developed antibody to rsv; 25 of the 30 initially seronegative children who did not develop antibody were less than 12 months old, which suggested that very low levels ...19827037983
comparison of direct and indirect immunofluorescence staining of clinical specimens for detection of respiratory syncytial virus antigen.immunofluorescence staining methods for respiratory syncytial virus antigen detection were compared. of 50 specimens originally positive for respiratory syncytial virus by direct immunofluorescence and culture, 49 were positive by repeat direct immunofluorescence and 32 were positive by indirect immunofluorescence. additional results obtained on specimens originally negative for respiratory syncytial virus by direct immunofluorescence, culture, or both indicate that direct immunofluorescence sta ...19827047562
consideration regarding infection during hospital employment.among the most important responsibilities of a hospital's employees' health service is the prevention or prompt identification of infectious illnesses among staff members. infections constitute a major health hazard of hospital employment and, both intrinsically or as a consequence of subsequent transmission to hospitalized patients, can have devastating consequences. the risks associated with tuberculosis, viral hepatitis, rubella, herpes, meningococcal disease, and scabies are generally acknow ...19827054483
atypical neonatal respiratory syncytial virus infection. 19827082462
respiratory syncytial virus pneumonia. 19827062498
in vivo inhibition of respiratory syncytial virus by ribavirin.ribavirin reduced the amount of respiratory syncytial virus in nasal turbinates and lung tissues of experimentally infected cotton rats by over 90%. an effect was seen when the drug was given either intraperitoneally or by aerosol; however, the antiviral effect was achieved at much lower doses when delivered by the aerosol route. no animal deaths due to the drug were seen.19827044296
clinical and pathological observations on spontaneous bovine respiratory syncytial virus infections in calves.a clinical, pathological and microbiological study was made of acute spontaneous bovine respiratory syncytial virus infection in calves. characteristic features were atelectatic areas, which had an exudative and, or, necrotising bronchiolitis with syncytial giant cells in the epithelial lining and in the lumina of these bronchioles. restricted to these areas both bronchiolar and alveolar immunofluorescence for bovine respiratory syncytial virus were seen. complications were severe interstitial o ...19826755591
experimental infection of lambs with bovine respiratory syncytial virus and pasteurella haemolytica: immunofluorescent and electron microscopic studies.colostrum-deprived lambs were inoculated transtracheally with respiratory syncytial virus (rsv), pasteurella haemolytica, or rsv and p haemolytica. multiple tissues were examined by immunofluorescence to localize viral and bacterial antigens, and lungs were examined by electron microscopy for cytopathologic changes. using immunofluorescence, viral antigen was detected only in the respiratory tract, mainly in the bronchial and bronchiolar epithelium and in the alveolar wall. lesser amounts of vir ...19827046531
antibody content of rabbit milk and serum following inhalation or ingestion of respiratory syncytial virus and bovine serum albumin. 19827200842
respiratory syncytial virus and otitis media with effusion. 19827201016
comparison of enzyme-linked immunosorbent assay and neutralization techniques for measurement of antibody to respiratory syncytial virus: implications for parenteral immunization with live virus vaccine.the sensitivity of an enzyme-linked immunosorbent assay (elisa) to detect low levels of antibody to respiratory syncytial (rs) virus was compared with a tube dilution neutralization test (neut) on sera obtained from children who received a parenteral live rs virus vaccine. among the children who developed antibody in response to live rs virus vaccine. elisa was as sensitive as neut at detecting antibody increases. some children who did not have detectable prevaccine elisa antibody possessed neut ...19827107002
experimental pneumovirus infections. 2. hydrocephalus of hamsters and mice due to infection with human respiratory syncytial virus (rs).respiratory syncytial virus (rs) injected into suckling hamsters and mice via the cerebral route induced hydrocephalus in 35% of the animals. the pathogenesis of the condition has been examined by light microscopy, immunofluorescence, and virus isolation. infection of leptomeninges and ependyma was revealed by immunofluorescence of virus-specific antigens between days 3 and 7 after inoculation. histologically, eosinophilic inclusion bodies were visible within the cytoplasma of ependymal and meni ...19827065124
appearance of complement components and immunoglobulins on nasopharyngeal epithelial cells following naturally acquired infection with respiratory syncytial virus.nasopharyngeal epithelial cells (npec) were collected from 144 infants and children with respiratory syncytial virus (rsv) infection, and were analyzed by fluorescent antibody techniques for the presence of cell-bound complement (c'3), iga, igg, and igm class of immunoglobulins (ig), and respiratory syncytial virus antigen. viral antigen was present on the surface of npec in 100% of samples obtained in the first 3 days or illness. the percentage of patients positive for rsv antigen declined stea ...19827069409
analysis of proteins synthesized in respiratory syncytial virus-infected cells.the spectrum of respiratory syncytial virus-encoded proteins was examined in infected cell extracts by standard polyacrylamide gel electrophoresis and by two-dimensional gel analysis. polyacrylamide gel electrophoresis analysis of a variety of respiratory syncytial virus-infected, actinomycin d-treated cell lines revealed the presence of as many as nine virus-encoded proteins. seven of these nine proteins were immunoprecipitated by anti-respiratory syncytial serum. only one major band of [3h]glu ...19827086963
respiratory syncytial viral infection in infants with congenital heart disease.occasional reports have suggested that infants with congenital heart disease may have an increased risk of severe illness from respiratory syncytial virus (rsv) infection. we prospectively studied 699 infants hospitalized during the winters of 1976 through 1980, when rsv was prevalent in the community; 229 of these infants had proved rsv infections acquired either before admission or during hospitalization; 27 had both congenital heart disease and rsv infection, and 46 had congenital heart disea ...19827088112
experimental infection of lambs with bovine respiratory syncytial virus and pasteurella haemolytica: pathologic studies.nineteen 4-week-old, colostrum-deprived lambs were transtracheally inoculated with respiratory syncytial virus (rsv), pasteurella haemolytica biotype a serotype 1, or rsv and p haemolytica. pneumonic lesions were more frequent, more extensive, and more severe in lambs inoculated with rsv and p haemolytica than in lambs inoculated with either agent alone. lesions were seen in 2 of 4 lambs inoculated with p haemolytica alone, in 3 of 4 lambs inoculated with rsv alone, and in 11 of 11 lambs inocula ...19827091824
experimental infection of lambs with bovine respiratory syncytial virus and pasteurella haemolytica: clinical and microbiologic studies.four-week-old lambs were inoculated transtracheally with respiratory syncytial virus (rsv), pasteurella haemolytica, or rsv and p haemolytica. when given in combination, rsv administration preceded p haemolytica by 3 or 5 days. lambs inoculated with p haemolytica or rsv developed a mild respiratory tract disease accompanied by a transient pyrexia in a few lambs. by 24 hours after inoculation of bacteria, all lambs inoculated with rsv and p haemolytica were listless, reluctant to move, and exhibi ...19827091825
apnoea and bronchiolitis due to respiratory syncytial virus. 19827103880
a natural outbreak of bovine respiratory disease caused by bovine respiratory syncytial outbreak of respiratory disease occurred in a herd of 34 calves and 5 cows in a barn. the disease which affected 24 calves and 1 cow, was characterized by coughing, fever, anorexia, nasal discharge and pulmonary rales. two calves died 5 days after the disease outbreak and were necropsied. bronchitis, bronchiolitis and alveolitis were among the lesions observed in these calves. bovine respiratory syncytial virus was detected, in lung sections of the dead calves and also in nasal washings of so ...19827105764
lack of relationship of community patterns of respiratory viral activity to systemic haemophilus influenzae disease in children.the occurrence of epidemics of respiratory viral illness was monitored by virus isolations in a population of young children living in the greater nashville area over a 4-year period. epidemic disease due to influenza, respiratory syncytial virus and parainfluenza type 1 was present during discrete time blocks over this interval. occurrence of systemic haemophilus influenzae type b disease in the community was also documented by review of bacteriologic records of vanderbilt children's hospital o ...19827163030
development of antibody-dependent cell-mediated cytotoxicity in the respiratory tract after natural infection with respiratory syncytial virus.antibody-dependent cell-mediated cytotoxicity (adcc) was measured in nasopharyngeal secretions collected from 42 infants and young children at various intervals after primary or secondary infection with respiratory syncytial virus. adcc was determined by specific immune release of 51cr from respiratory syncytial virus-infected hep-2 cell culture monolayers, with lymphocytes from adult volunteers as effector cells. specific adcc responses in nasopharyngeal secretions after primary infection were ...19827118247
virus-cell interactions: nuclear magnetic resonance behaviour of intracellular water.the water proton nmr spin-lattice relaxation time if measured in hep-2 cells in relation to the infection by different viruses (poliovirus type 1, coxsackievirus b-3, human adenovirus, measles virus, respiratory syncytial virus, herpes simplex virus, subtype 1, and vaccinia virus). the nmr properties of intracellular water allow the early detection and identification of viruses. a close relationship is shown between the nmr behavior and the mode of virus-cell interaction.19827121298
[detection of antibodies to respiratory syncytial virus in a neonate with multiple abnormalities]. 19827125129
antibody against viruses in maternal and cord sera: specific antibody is concentrated on the fetal side of the circulation.paired maternal and cord sera from 100 pregnancies were tested for antibodies against herpes simplex virus, measles virus and respiratory syncytial virus by complement fixation and for antibodies against rubella virus, influenza a virus and influenza b virus by haemagglutination-inhibition. for four viruses (herpes simplex, measles, respiratory syncytial and rubella) higher levels of antibody were found in cord than in maternal sera. there was no difference between maternal and cord serum titres ...19827130705
clinical evaluation of radioimmunoassay of nasopharyngeal secretions and serology for diagnosis of viral infections in children hospitalized for respiratory infections.viral diagnosis was performed using radioimmunoassay (ria) for virus antigen in nasopharyngeal secretions (nps) and complement-fixation (cf) tests of paired sera from specimens of 90 children hospitalized for acute respiratory infection. major respiratory viruses sought for by both methods (adenoviruses, influenza a and b viruses, parainfluenza virus type 3, respiratory syncytial virus) were detected in 40 (44%) of the patients; 15% of the diagnoses were made by nps-ria alone. serologic diagnosi ...19827163028
the association of apnea and respiratory syncytial virus infection in infants. 19827086623
radioimmunoassay of measles virus hemagglutinin protein g.guinea pig and rabbit antisera from animals immunized with purified measles virus hemagglutinin (g) protein were used to establish a solid-phase four-layer radioimmunoassay for quantitative measurement of the g protein. the sensitive of the assay was 2 ng of purified g protein, and 200 micrograms of protein from uninfected vero cells neither decreased the sensitivity nor reacted non-specifically in the assay. radioimmunoassay standard dose-response curves were established and unknown values inte ...19827130334
immunochemical identification of viral and nonviral proteins of the respiratory syncytial virus virion.we identified by immunochemical methods 13 polypeptides associated with the infectious respiratory syncytial virus virion. eight of these polypeptides (vp200, vp84, vp66, vp43, vp40, vp37, vp28, and vp19) were identified as virus specific. two other polypeptides, (vp) 22 and (vp) 12, are provisionally considered to be of viral origin. three nonviral proteins are also intimately associated with the infectious virion. these nonviral proteins were identified as cellular actin and two proteins with ...19827107006
administration of a highly attenuated, live respiratory syncytial virus vaccine to adults and children.a highly attenuated respiratory syncytial virus (rsv) experimental vaccine, rsv ts-2, was sequentially evaluated in adults, seropositive children, and finally, fully susceptible seronegative children. the vaccine was administered intranasally in doses ranging from 10(5.2) to 10(6.3) pfu/ml. in both adults and children, the vaccine proved to be poorly infectious. although poor infectivity would not have been predicted from tissue culture studies of rsv ts-2 growth, the human experience closely pa ...19827107009
[encephalopathy producing antibodies against respiratory syncytial virus in the central nervous system in an adult woman]. 19827164044
detection of respiratory syncytial virus in nasopharyngeal secretions by elisa: comparison with fluorescent antibody technique.samples of nasopharyngeal secretions (nps) obtained from 140 infants and children with acute respiratory disease were examined for the presence of respiratory syncytial (rs) virus by elisa. an antiserum produced in rabbits against rs-virus polypeptides was used both as the "capture" antibody and as conjugate in a double-antibody sandwich technique. rs virus was detected by elisa in specimens of nps obtained from 72 (51%) of the patients. by comparison rs virus was shown to be present in 86 (61%) ...19827161598
respiratory syncytial virus infections in children. 19827183846
experimental viral early, acute arthritis was observed in 8 of 22 mice experimentally infected with respiratory syncytial virus. no abnormalities were found in the joints of the 12 control mice. the pathological changes in joints of the infected animals included pannus formation and erosion, resembling changes that occur in arthritis in man, including rheumatoid arthritis. it is proposed, therefore, that viruses may play an aetiological role in acute and chronic arthritis in man. these investigations provide a ...19827093144
serological evidence for the intervention of bovine respiratory syncytial virus in a respiratory disease outbreak in moroccan cattle. 19827090648
fatal haemorrhagic pneumonia in an adult due to respiratory syncytial virus and staphylococcus aureus. 19827185980
an indirect haemadsorption procedure (mrspah) for detecting antibodies to respiratory syncytial virus.a simple haemadsorption procedure for the detection of isotype specific antibodies to r.s. virus has been developed. correlation between detection of r.s. virus igg antibodies by mixed reverse (solid phase) passive antiglobulin haemadsorption (mrspah) and other serological procedures has been obtained. iga antibody was detected in 62% of milk samples studied. the method is rapid, simple and uses commonly available serological reagents.19826759520
cell-mediated immune response to respiratory syncytial virus infection in owl monkeys.the suitability of owl monkeys as experimental models to study the cellular immune response to respiratory syncytial virus (rsv) infection was examined. seronegative owl monkeys inoculated intranasally with rsv shed large quantities of virus and developed clinically evident upper respiratory disease. rsv infected monkeys had significant lymphoproliferative responses to rsv antigen by 4 weeks post-infection. in contrast, no positive blastogenic responses were elicited during the acute phase of il ...19836883807
identification of the virus-specific proteins of respiratory syncytial virus temperature-sensitive mutants by immunoprecipitation.the proteins of long strain rsv and three temperature-sensitive (ts) mutants of the a2 strain were compared by pulse labeling virus-infected cells with [35s]methionine and [3h]glucosamine followed by analysis of the cell lysates by polyacrylamide gel electrophoresis. at the permissive temperature (30 degrees) proteins ranging in molecular weight from 24,000 to 50,000 (vp24, vp27, vp33, vp44) could be identified. immunoprecipitation of viral lysates by immune rabbit serum demonstrated antigenic s ...19836828465
antibodies to respiratory syncytial virus polypeptides and their significance in human infection.the human antibody response to respiratory syncytial (rs) virus infection was investigated using radioimmunoprecipitation analysis (ripa). a total of nine rs virus-specific proteins, vp200, vgp95, vp68, vgp48, vpn41, vp35, vp27, vp23 and vgp20 were identified by comparing 35s- or 3h-labelled extracts of infected and uninfected hep-2 cells, and by radioimmunoprecipitation using a hyperimmune human serum. three glycopeptides, vgp95, vgp48 and vgp20, were identified by incorporation of [3h]glucosam ...19836886679
viral and bacterial pathogens of suspected sepsis in young infants.clinical and laboratory data of 27 previously normal, healthy infants 2 months of age or less who were hospitalized for suspected sepsis during a 1-year period were reviewed. results of bacterial cultures and viral studies revealed enteroviruses (seven echoviruses, one coxsackievirus) to be the predominant pathogens identified. other viruses identified were one each of adenovirus, untypeable hemadsorbing virus and respiratory syncytial virus. group b streptococcal and enterococcal bacteremia and ...19836889156
[infections in adults and in the elderly caused by respiratory syncytial virus]. 19836866148
isolation of respiratory syncytial and influenza viruses from the sputum of patients hospitalized with pneumonia.routinely collected sputum specimens from 100 adults hospitalized with pneumonia were frozen at -70 c until inoculation into madin-darby canine kidney, fetal tonsil, and esophageal epithelial cells. six influenza a (h3n2) viruses, two respiratory syncytial viruses, three rhinoviruses, and nine herpes simplex viruses were recovered. four patients with influenza virus and one with respiratory syncytial virus isolated had nosocomial pneumonia. viral isolation from sputum specimens may aid the diagn ...19836827134
[isolation of the respiratory syncytial virus in bronchiolitic complications of whooping cough. preliminary research]. 19836877195
presumed respiratory syncytial virus pneumonia in three immunocompromised adults.three cases of presumed respiratory syncytial virus (rsv) pneumonia in immunocompromised adults are described. two patients had symptoms of cough, fever, and malaise, following completion of a course of combination chemotherapy for the treatment of acute lymphoblastic leukemia. the third patient, a juvenile onset diabetic, developed similar symptoms while hospitalized for severe hyperglycemia. chest roentgenograms showed lower lobe infiltrates in both leukemic patients and a bilateral non-conflu ...19836846393
inoculation of hep-2 cells for respiratory syncytial virus isolation. 19836848291
respiratory syncytial virus polypeptides. iii. the envelope-associated proteins.four proteins, gp1, vgp48, gp26 and vpm27, are associated with the envelope of respiratory syncytial (rs) virus. the status of gp1 has been uncertain, because a cellular glycoprotein migrates at the same position when laemmli's discontinuous buffer system is used for page, and because bsc-1 cells infected with the rsn-2 strain of rs virus appear not to contain gp1. however, additional evidence suggests that gp1 is a viral structural protein. (i) it is removed from cells by trypsin, while the cel ...19836834007
effect of maternal nutritional status on immunological substances in human colostrum and milk.substances in colostrum and breast milk confer significant disease resistance to the breast-fed infant. the influence of maternal nutritional status on both immunological and nonimmunological milk factors was studied in a group of 23 colombian women during the first 2 months of lactation. maternal malnutrition was characterized by significantly lower weight/height ratio, creatinine/height index, total serum proteins, serum albumin, and serum igg and iga. the colostrum of malnourished mothers con ...19836837495
replication of respiratory syncytial virus in lungs of immunodeficient mice.respiratory syncytial virus was frequently isolated during a 10-day test period from the lungs of 4- to 6-week-old immunodeficient nude (nu/nu) mice and from gamma-irradiated c3h mice inoculated intranasally with this virus, but not from similar aged and comparably inoculated normal littermates of these mice. virus isolation rates and levels of virus in lungs in both groups of immunodeficient mice were similar. no extrapulmonary dissemination of virus was observed in any test group of mice.19836887156
a colorimetric assay for quantification of defective interfering particles of respiratory syncytial virus.a colorimetric assay for defective interfering (di) particles of respiratory syncytial (rs) virus was developed. this quantitative biological assay is based on neutral red dye uptake by di particle-protected cells that survive standard virus challenge. this assay was more sensitive than the reduction of infectious yield (riy) assay and was capable of detecting 1 x 10(4) to 2 x 10(4) di particles/ml. the coefficient of variation for parallel, simultaneous replicates (n = 10) was 23%. cell-protect ...19836854271
[respiratory syncytial virus in bovine practice]. 19836857220
respiratory syncytial virus infection in young hospitalized children. identification of risk patients and prevention of nosocomial spread by rapid diagnosis.detection of rsv by immunofluorescence was used for rapid confirmation of the diagnosis in 89 infants during an epidemic. this made it possible to plan the care of the patients and use the personnel in an optimal way in order to maintain nosocomial infection at a low level. somatic and environmental risk factors connected with serious disease were also identified. the course of the disease was more severe in children with chronic illnesses or those who had had perinatal complications. allergy wa ...19836858684
construction and characterization of cdna clones for four respiratory syncytial viral genes.cytoplasmic poly(a)-containing rna from respiratory syncytial virus-infected cells was used as a template to synthesize oligo(dt)-primed cdnas. discrete size classes of single-stranded cdnas, resolved by alkali agarose gel electrophoresis, were used separately to construct double-stranded cdnas that were subsequently inserted into the plasmid vector pbr322 at the pst i site by means of oligo(dg)oligo(dc) tailing. after transfection of escherichia coli, recombinant plasmids were screened mostly b ...19836572388
characterization of the human peripheral blood effector cells mediating antibody-dependent cell-mediated cytotoxicity against respiratory syncytial virus.peripheral blood lymphocytes were separated into several subpopulations and evaluated for their ability to mediate antibody-dependent cell-mediated cytotoxicity (adcc) against respiratory syncytial virus (rsv)-infected hela cells. using erythrocyte rosetting methods, nylon wool filtration, and cytolysis with okt-3 monoclonal antibody, two lymphocyte subpopulations were shown to mediate rsv-adcc; non-t, non-b, and igg-fc receptor-bearing lymphocytes and e-rosetting cells with iggfc receptors (t g ...19836603313
[asthmatic syndrome in respiratory syncytial virus infection associated with influenza in young children]. 19836356010
ribavirin aerosol treatment of bronchiolitis associated with respiratory syncytial virus infection in a double-blind study, bronchiolitis associated with respiratory syncytial virus infection in 12 randomly selected patients treated with ribavirin aerosol improved more rapidly than in 14 control patients given saline aerosol (p = .044, wilcoxon rank sum test, two-tailed). an estimated 10 mg of ribavirin per kilogram of body weight was administered in daily 12-hour treatments over a five-day period. respiratory syncytial virus disappeared from secretions at about the same rate in treated and c ...19836356005
cellular and humoral immunity in recurrent respiratory syncytial virus infections. 19836622111
indirect haemagglutination test for the detection and assay of antibody to bovine respiratory syncytial indirect haemagglutination (iha) test was used for the rapid assay of antibody to bovine respiratory syncytial virus. antigens for the sensitisation of formalised tanned erythrocytes were prepared by treatment of virus infected cells with non-ionic detergent. a close serological relationship was shown by the iha test between the strain of bovine respiratory syncytial virus used and the a2 strain of human respiratory syncytial virus. the iha test was sensitive and reproducible. a linear correl ...19836636489
mechanisms of immunity to respiratory syncytial virus in cotton immunity and maternally transmitted passive immunity to respiratory syncytial virus (rsv) were studied in cotton rats. animals infected with respiratory syncytial virus developed complete resistance to pulmonary reinfection, which lasted at least 18 months. nasal resistance was of shorter duration and began to diminish in 8 months. pulmonary resistance was transferred by parabiosis, but nasal resistance was not. adoptive transfer studies with fractionated convalescent blood showed that se ...19836352505
respiratory syncytial virus. 19836662671
respiratory syncytial virus pneumonia in young calves: clinical and pathologic findings.nine young calves given respiratory syncytial virus by a combined intranasal and intratracheal route developed a severe respiratory tract disease in which coughing, tachypnea, and hyperpnea were prominent clinical features. calves were euthanatized on postinoculation (initial) days (pid) 1 to 13. at necropsy, large areas of consolidation were present in the cranial, middle, accessory, and cranial parts of the caudal lung lobes of calves killed between pid 4 and 13. histopathologic examination re ...19836625318
modified immunoprecipitation procedure for the identification of human respiratory syncytial virus polypeptides.when analysed by polyacrylamide gel electrophoresis, human respiratory syncytial virus harvested after a one step growth cycle and purified through a continuous sucrose density gradient was shown to be composed of nine structural proteins of 90, 68, 49, 42, 34, 28, 25, 19 an 13 kd. the 90, 49 and 19 kd polypeptides were identified as glycopolypeptides by glucosamine incorporation. a modified immunoprecipitation procedure confirmed the viral specificity of the 49, 42, 28, 25 and 19 kd polypeptide ...19836643642
a study of respiratory infections in the elderly to assess the role of respiratory syncytial virus.a prospective study was performed from 1 december 1981 to 31 may 1982 in two departments of geriatric medicine in edinburgh. this yielded 159 cases of acute respiratory tract infection (rti). twelve of these were undoubtedly associated with respiratory syncytial virus (rsv), 14 with influenza a and 18 with influenza b (as established in each case by a fourfold or greater increase in antibody titre). eighty-five of the 159 patients with rti and rsv titres of 32 or greater. their significance is d ...19836663083
[investigation on the level of antibodies against respiratory syncytial virus in children in nanning city]. 19836675846
serology for diagnosis and epizootiological studies of bovine respiratory syncytial virus infections.the complement fixation test (cft) and the virus neutralisation test (vnt), performed as a plaque reduction test, were employed to measure antibodies to bovine respiratory syncytial virus. the cft with bovine sera was performed with supplementation of the complement factors in fresh guinea pig serum by an adequate amount of clq-factor of the bovine species. kinetics of maternally derived antibodies and the antibody response after spontaneous and experimental infections and after intramuscular va ...19836635341
[hospital epidemic outbreak of respiratory syncytial virus]. 19836676858
respiratory syncytial virus mrna coding assignments.the polypeptide coding assignments for six of the respiratory syncytial virus-specific mrnas were determined by translation of the individual mrnas in vitro. the coding assignments of the rnas are as follows. rna band 1 is complex and can be separated into at least two components on the basis of electrophoretic mobility (molecular weights [mws] approximately equal to 0.21 x 10(6) and 0.31 x 10(6), respectively) that code for three polypeptides of 9.5, 11, and 14 kilodaltons (k). rna 2 (mw, 0.39 ...19836682456
total serum ige levels in venezuelan schoolchildren.seventy-eight disease-free children were evaluated by prist for total serum ige in order to establish the normal range for this immunoglobulin and assess its utility, in tropical climates, such as venezuela, as a diagnostic tool for clinical allergy. seventy-eight normals were selected from a group of 1053 children, aged 7-12 years from fourteen schools in caracas. exclusion from the normal pool was based on nationality, and on historical, clinical, and/or laboratory evidence of atopic and/or in ...19836640887
rapid diagnosis of respiratory syncytial virus and influenza a virus infections by immunofluorescence: experience with a simplified procedure for the preparation of cell smears from nasopharyngeal secretions.a simplified procedure for preparing direct smears of exfoliated cells from nasopharyngeal secretions is presented. the specimens were examined by the indirect immunofluorescence (if) technique for the presence of respiratory syncytial (rs) virus and influenza a virus antigens. specimens from 457 children hospitalised at twelve different hospital departments in norway were examined during the winter season 1980-81. rs virus was identified in samples from 139 children and influenza a virus from 2 ...19836353858
enzyme-linked immunosorbent assay for serum antibody to bovine respiratory syncytial virus: comparison with complement-fixation and neutralization indirect enzyme-linked immunosorbent assay (elisa) for the detection of serum antibody to bovine respiratory syncytial virus (brsv) was developed and compared with complement-fixation (cf) and viral neutralization (vn) tests. tissue culture-grown viral antigens were used in the tests. serum samples from various sources were compared, including serum samples from 10 calves which were infected experimentally with brsv by aerosol exposure. the elisa compared favorably with the vn test for detect ...19836660613
respiratory syncytial virus pneumonitis in adults.two cases of respiratory syncytial virus pneumonitis in adults area described. in both patients, the clinical picture of the adult respiratory distress syndrome, with marked tachypnoea. hypoxaemia and bilateral diffuse pulmonary infiltrates, was present. one patient had systemic lupus erythematosus, while the other had chronic obstructive lung disease and was a heavy drinker of alcohol. both patients survived and recovered after a prolonged stay in hospital.19836835164
polymorphonuclear function and respiratory syncytial virus infections in children. 19836618679
viral infections in clinical pertussis.of 44 children with clinical pertussis 29 (66%) had serological evidence of concurrent infection with respiratory viruses or mycoplasma pneumoniae. the commonest superinfection was by mycoplasma (32%), followed by respiratory syncytial virus (27%) and adenoviruses (16%). the high prevalence of viral infections, many being multiple, supports the theory that pertussis predisposes to such infections or vice versa.19836623245
respiratory syncytial virus glycoproteins.the proteins of respiratory syncytial (rs) virus were analyzed by sds-polyacrylamide gel electrophoresis. eight virion structural proteins with molecular weights of 180,000, 89,000, 48,000, 42,000, 34,000, 28,000, 25,000, and 21,000 were identified. these proteins were given tentative designations of l (180,000), g (89,000), f1 (48,000), np (42,000), p (34,000), m (28,000), vp25 (25,000), and f2 (21,000). the 89,000-, 48,000-, and 21,000-dalton polypeptides were glycosylated and could be purifie ...19836636537
report of a workshop on respiratory syncytial virus and parainfluenza viruses. 19836194232
cell-mediated cytotoxic responses in lungs of cotton rats infected with respiratory syncytial virus.pneumonia induced in cotton rats (sigmodon hispidus) after inoculation of respiratory syncytial virus (rsv) was accompanied by the appearance of leukocytes in infected lungs. the number of these leukocytes increased until day 5 after inoculation when sixfold more leukocytes were recovered from infected lungs using transpleural lavage than were recovered from lungs of uninfected control animals. although macrophages and lymphocytes constituted approximately 65 and 25%, respectively, of the cells ...19836340570
correlation of cytotoxic activity in lungs to recovery of normal and gamma-irradiated cotton rats from respiratory syncytial virus infection.cotton rats (sigmodon hispidus) that were exposed to 300, 600, or 900 rads of gamma irradiation and inoculated intranasally 2 days later with respiratory syncytial virus (rsv) exhibited prolonged virus shedding and delayed humoral and cytotoxic immune responses compared with comparably inoculated nonirradiated control rats. in nonirradiated animals and in animals exposed to 300 and 600 rads, levels of virus declined and then disappeared from the lungs during the period in which cytotoxic activit ...19836354026
monoclonal antibodies to respiratory syncytial virus proteins: identification of the fusion protein.six monoclonal antibodies directed against respiratory syncytial virus proteins were produced. each was characterized by immunoprecipitation and indirect immunofluorescence. one was directed against the nucleocapsid protein. np 44, two were directed against a 37,000-dalton protein, two were directed against the major envelope glycoprotein, gp 90, and one was directed against the 70,000-dalton envelope protein, vp 70. indirect immunofluorescence stain patterns of infected hep-2 cells defined gp 9 ...19836345804
viruses associated with acute respiratory infections in children admitted to hospital in naples, 1979-82.a survey of the virological and epidemiological features of acute respiratory diseases in children admitted to hospital in naples has been carried out; the results of three years of research are reported.between april 1979 and march 1982, 787 nasopharyngeal swabs were examined. there were 287 (36.5%) positive samples, with the highest isolation rate being found in children with bronchiolitis (39.5%).among the different viruses isolated, adenovirus was the most common (161 positive samples, 56%); ...19836325032
ribavirin treatment of experimental respiratory syncytial viral infection. a controlled double-blind study in young adults.the effect of ribavirin aerosol on experimental respiratory syncytial virus (rsv) infection was evaluated in a double-blind controlled study of 16 young adult volunteers. two days after intranasal inoculation with rsv, half of the subjects were treated with a small-particle aerosol of ribavirin and half with placebo for a total of 12 hours each day for three days. seven of eight placebo-treated and six of eight ribavirin-treated subjects became infected. viral shedding was diminished in the riba ...19836341640
aerosolized ribavirin treatment of infants with respiratory syncytial viral infection. a randomized double-blind study.we evaluated a new antiviral agent, ribavirin, in the treatment of infants hospitalized with lower-respiratory-tract disease from respiratory syncytial virus. ribavirin or placebo was administered to 33 infants in a double-blind manner by continuous aerosol for three to six days. seventeen infants were treated with placebo, and 16 with ribavirin. by the end of treatment, infants receiving ribavirin had significantly greater improvement in their overall score for severity of illness, in lower-res ...19836343860
the nosocomial spread of respiratory syncytial viral infections.respiratory syncytial virus is a regular winter visitor that is highly contagious among persons of all ages. it is the major nosocomial pathogen on infant and toddler wards, and has recently been recognized as also causing appreciable nosocomial illness in the elderly. control of the spread of this virus has been difficult, but transmission appears to require close contact via large-particle aerosols or via fomites. environmental conditions affect the survival of the virus on varying surfaces an ...19836344761
hotting-up the complement-fixation test.a detailed investigation into the effect of modifying the incubation temperature of the complement-fixation (cf) test is described. for varicella-zoster virus cytomegalovirus and rubella virus, increasing the incubation temperature progressively increased the sensitivity of the cf test to reach a maximum at 15 degrees c, at which temperature the geometric mean titre of seropositive samples was significantly greater than that found at 4 degrees c. for these three viruses, each serum shown to cont ...19836296229
[chronic isolated brain lesions caused by respiratory viruses in children].combined study of 48 postmortem observations of cerebral lesions caused mainly by meningococcal infection in children was carried out. in 15 cases, isolated subclinical involvement of the brain (more frequently, of the vascular plexus and meninges) caused by influenza a viruses (8 cases), b (2 cases), adenoviruses (6), parainfluenza and respiratory syncytial virus (1 each), and chlamydia (2) was found. this was proved by the presence of the appropriate antigens in the brain revealed by immunoflu ...19836309121
viral infections of the respiratory tract in hospitalized children. a study from oslo during a 90 months' period.a diagnosis of 979 respiratory viral infections was made in hospitalized children. respiratory syncytial virus greatly out-numbered the other viruses: it caused 58% of the total virus infections and occurred in winter epidemics. influenza a and b virus occurred during late winter and spring, rhinovirus had a seasonal distribution towards spring and autumn, whereas adenovirus types 1, 2 and 5 had no distinct seasonal distribution. whereas respiratory syncytial virus were mainly associated with br ...19836305106
rapid detection of respiratory syncytial virus with a monoclonal antibody.we developed an indirect fluorescent-antibody test employing a mouse monoclonal antibody directed against the nucleoprotein of rsv for rapid detection of respiratory syncytial virus (rsv). this test demonstrated distinctive fluorescent inclusions in hep-2 cells infected with 24 rsv isolates collected during 6 previous years but not in cells infected with 13 other respiratory viruses. examination of nasal cells of 100 infants with acute respiratory illness showed that the indirect fluorescent-ant ...19836348077
elimination of contaminating bovine viral diarrhea virus from bovine respiratory syncytial virus stock. 19836306037
croup: an 11-year study in a pediatric practice.the etiology and epidemiology of croup were studied in a pediatric group practice over an 11-year period, 1964 to 1975. croup was diagnosed in 951 instances in 6,165 cases of lower respiratory tract infection (lri) studied. as census figures of the practice clientele were available, attack rates were calculated. the incidence of total lri was highest in the first year of life. in contrast, the attack rate for croup was highest in the second year of life; the rate declined gradually after that ag ...19836304611
improvement of respiratory syncytial virus replication in actively growing hep-2 cells.the growth of respiratory syncytial (rs) and parainfluenza type iii (pi3) viruses has been studied in actively growing versus relatively stationary hep-2 cells. there was no effect on pi3 virus growth. rs virus synthesis was stimulated from 20- to 60-fold by growth in actively growing cultures when the cell density was approximately 1/3 that of a confluent culture. this stimulation was manifested by a greater yield of virus per cell, more virus-specific mrna produced per cell, and a 50% decrease ...19836317708
[viruses and asthma].more than one-third of infants with respiratory syncytial virus (rsv) bronchiolitis subsequently experience recurrent episodes of wheezing. in asthmatic children, viral infections frequently trigger asthmatic attacks. indeed, viruses induce immunological and respiratory functional disorders. as compared with non-wheezing rsv infected infants, wheezing infants with bronchiolitis or asthma due to rsv have: a) increased t-lymphocyte responsiveness to rsv antigen; b) more persistent ige bound to nas ...19836320377
antiviral drugs 1983.a number of antiviral compounds are currently available, and several others are of great interest. trifluridine, idoxuridine, and vidarabine are effective topically in herpes simplex virus keratoconjunctivitis infection. vidarabine (and presumably acyclovir) is effective in herpes simplex virus encephalitis and in herpes zoster infections in the immunocompromised host. acyclovir is effective topically, orally, and intravenously in primary herpes genitalis, and the oral and intravenous forms are ...19836194388
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