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infection of british deer and farm animals with epizootic haemorrhagic disease of deer virus. 1977198436
epizootic haemorrhagic disease of deer virus in tissue culture and laboratory animals. 1977198437
possible introduction of epizootic hemorrhagic disease of deer virus (serotype 2) and bluetongue virus (serotype 11) into british columbia in 1987 and 1988 by infected culicoides carried on the wind.outbreaks of epizootic hemorrhagic disease of deer and of bluetongue began in british columbia in august and october 1987 respectively and recrudescence of infection by both viruses was detected the following year in august. weather records for up to 18 days before the initial outbreaks of disease, isolation of virus or seroconversion were examined to determine if the viruses could have been introduced by infected culicoides carried on the wind. data on temperature, rainfall, wind speed and dire ...19911665099
epidemiology of bluetongue and related orbiviruses in the sultanate of oman.sentinel herds at 34 farms were used to study the epidemiology of bluetongue and related orbiviruses in oman. the results indicate that bluetongue virus (btv) is widespread and is enzootic in northern oman. at least three btv serotypes (3, 4 and 22) were present at the time of the study. antibodies to epizootic haemorrhagic disease of deer virus (ehdv) type 2 and ehdv-318 were also detected but were less prevalent. entomological investigations identified the presence of 16 species of culicoides. ...19911847102
dot immunoperoxidase assay using monoclonal antibody for detection of bluetongue virus antigens.a rapid, simple dot immunoperoxidase assay (dipa) is described for visual detection and identification of bluetongue virus (btv) antigens in samples of infected cell culture fluid. the assay was performed using nitrocellulose (nc) paper and 'dipsticks'. dots of samples were adsorbed to the nc surface and the remaining non-specific binding sites were blocked with skim milk solution. btv was detected with either of two murine monoclonal antibodies (4h4, 5g12) to the major group specific antigens o ...19911849912
phylogenetic analyses of the complete nucleotide sequence of the capsid protein (vp3) of australian epizootic haemorrhagic disease of deer virus (serotype 2) and cognate genes from other orbiviruses.the complete nucleotide sequence of the minor capsid protein (vp3) of epizootic haemorrhagic disease of deer virus (ehdv; australian serotype 2) was determined using a combination of cloning and sequencing methods. gene segment 3 that coded for the ehdv vp3 capsid protein was 2768 nucleotides in length with a coding region of 2697 nucleotides flanked by 5' and 3' non-coding regions of 17 and 53 nucleotides, respectively. a protein of 899 amino acids (mr 103,160) having no overall charge at neutr ...19911962502
biochemical characterisation of australian orbiviruses.there are over 30 antigenically distinct orbiviruses found in australia, including members in the bluetongue virus (btv) and epizootic hemorrhagic disease of deer virus (ehdv) serogroups. genomic rna profiles were analysed by polyacrylamide gel electrophoresis (page) on both 10% laemmli and tris-borate-edta-(tbe)-urea gels. there was considerably more variation in the rna profiles in laemmli gels than was apparent in the tbe-urea gels. since the latter system separates on molecular size, then pr ...19852989870
bluetongue virus and epizootic haemorrhagic disease of deer virus serotypes in northern colombian cattle.there is recent evidence of bluetongue (bt) and epizootic haemorrhagic disease (ehd) virus infection of cattle in the american tropics, including bt group reactive antibody in colombian cattle. these observations prompted a study to determine serologically the specific bt and ehd virus types present, and time of infection and to collect culicoides spp. as potential vectors. a prospective study of bt and ehd virus infection was done on two farms in the colombian department of antioquia. sequentia ...19852991365
the complete nucleotide sequence of bluetongue virus serotype 1 rna3 and a comparison with other geographic serotypes from australia, south africa and the united states of america, and with other orbivirus isolates.the sequence of the rna segment 3 of bluetongue virus (btv) serotype 1 from australia is presented along with its deduced amino acid sequence. dna copies of this genome segment were inserted either into the e. coli plasmid pbr322 by homopolymeric tailing or by direct insertion of double-stranded dna fragments generated by restriction endonuclease cleavage into the appropriate m13 bacteriophage vectors (vieira, j. and messing, j., 1982, gene 19, 259-268). direct comparisons were made to the nucle ...19873035818
monoclonal antibody analysis of serotype-restricted and unrestricted bluetongue viral antigenic determinants.twenty-one monoclonal antibodies that react with bluetongue virus (btv) and have restricted or unrestricted serotype specificities were identified in culture supernatants of hybridomas derived from lymphocytes of mice immunized with btv serotype 17. hybridomas were screened and antibody specificities characterized in a solid-phase radioimmunoassay and by immunoprecipitation with radiolabeled, btv 17-infected cell lysates. three general serotype specificities were demonstrated by 13 antibodies th ...19836186076
identification of epizootic haemorrhagic disease of deer virus serotypes using a fluorescence inhibition test.a fluorescence inhibition test (fit) is described for serotyping rapidly isolates of epizootic haemorrhagic disease of deer virus (ehdv). the test used a serogroup-reactive monoclonal antibody in a immunofluorescence procedure to detect virus which resisted neutralisation by antisera to any of the eight known ehdv serotypes. the ehdv fit provided an accurate serotype identification procedure for all eight reference serotypes and, in comparison with the plaque inhibition assay, abbreviated the se ...19948188818
a competitive elisa for the detection of anti-tubule antibodies using a monoclonal antibody against bluetongue virus non-structural protein ns1.a monoclonal antibody directed against the largest bluetongue virus (btv) non-structural protein (ns1) was used in a competitive elisa to detect antibodies to tubules (composed of ns1) in serum samples. anti-tubule antibodies were detected at approximately 10 days post-infection in btv infected sheep but no such antibodies were detected in sheep injected with inactivated btv vaccines. tubules are also produced during the replication of other related orbiviruses, including epizootic haemorrhagic ...19938396154
evaluation of a commercial competitive elisa test kit for the detection of group-specific antibodies to bluetongue virus.the performance of a competitive (c) enzyme-linked immunosorbent assay (elisa) test kit, blueplate special (bps), commercially produced by diagxotics (wilton, ct) for detection of group-specific antibodies to bluetongue virus (btv) was compared with that of an internationally endorsed celisa-i. a total of 1,026 serum samples were tested in this study: 133 samples from 23 calves and 3 sheep experimentally infected with south african isolates of 19 btv serotypes and us isolates of 5 btv serotypes ...19938396982
competitive elisa for the detection of antibodies against epizootic haemorrhagic disease of deer virus.a competitive enzyme-linked immunosorbent assay is described for the detection of antibodies against epizootic haemorrhagic disease of deer viruses (ehdv). test antisera were tested against a guinea-pig antiserum raised against ehdv core particles. the assay detected antibodies to all serotypes of ehdv, but unlike the agar gel immunodiffusion (agid) test, gave no cross-reactions with antibodies against bluetongue, palyam and tilligery viruses. the c-elisa would be ideal for use in epidemiologica ...19958609206
monoclonal antibody based competitive elisa for the detection of antibodies against epizootic haemorrhagic disease of deer virus.a monoclonal antibody based competitive enzyme-linked immunosorbent assay (mc-elisa) for the detection of antibodies against epizootic haemorrhagic disease of deer viruses (ehdv) is described. test sera were competed with a monoclonal antibody against the vp7 protein of ehdv serotype 1. the assay was capable of detecting antibodies to all serotypes of ehdv but unlike the agar gel immunodiffusion (agid) test, gave no cross-reactions with antibodies against bluetongue, palyam or tilligery viruses. ...19968801224
internal image rabbit anti-idiotypic antibody detects sheep antibodies to the bluetongue virus core protein vp7.rabbit polyclonal anti-idiotypic antibodies (anti-id) were generated against three murine monoclonal antibodies (mabs) specific for the group-specific antigen vp7 of bluetongue virus (btv) by the sequential immunization method. it was demonstrated by serological tests that part of the anti-id possesses the characteristics of internal image anti-id. by affinity purification against the individual mabs, the internal image anti-id, designated rab2-a, was isolated and identified as specific for the ...19959373343
development of an enzyme-linked immunosorbent assay for the detection of antibody to epizootic hemorrhagic disease of deer virus.an enzyme-linked immunosorbent assay has been developed to detect antibodies to epizootic hemorrhagic disease of deer virus (ehdv). the assay incorporates a monoclonal antibody to ehdv serotype 2 (ehdv-2) that demonstrates specificity for the viral structural protein, vp7. the assay was evaluated with sequential sera collected from cattle experimentally infected with ehdv serotype 1 (ehdv-1) and ehdv-2, as well as the four serotypes of bluetongue virus (btv), btv-10, btv-11, btv-13, and btv-17, ...200010730943
effect of temperature on the transmission of orbiviruses by the biting midge, culicoides sonorensis.the influence of temperature on the likelihood of culicoides sonorensis wirth & jones (diptera: ceratopogonidae) transmitting african horse sickness virus (ahsv) serotypes 4 and 6, bluetongue virus (btv) serotypes 10 and 16 and epizootic haemorrhagic disease of deer virus (ehdv) serotype 1 was investigated. extrinsic incubation periods (eip), vector competence and vector survival were determined at 15, 20, 25 and 30 degrees c. the effect of humidity on vector survival was also investigated by ma ...200212109708
bluetongue virus in the french island of reunion.this paper records the results of a bluetongue virus (btv) serological survey and reports the first isolation of btv on the french island of reunion. in january 2003, the french island of reunion, located off the coast of madagascar, reported an outbreak of disease in cattle that resembled clinical bluetongue (bt) in sheep. the suspected causal agent was isolated and identified as epizootic haemorrhagic disease of deer virus (ehdv). however, because of the similarity in the clinical signs to tho ...200515778021
pcr detection of african horse sickness virus serogroup based on genome segment three sequence analysis.a nested reverse transcriptase (rt) polymerase chain reaction (rt-pcr), for rapid detection of african horse sickness virus (ahsv) double-stranded ribonucleic acid (dsrna) in cell culture and tissue samples, was developed and evaluated. using an outer pair of primers (p1 and p2), selected from genome segment three of ahsv serotype 6 (ahsv-6), the rt-pcr-based assay resulted in amplification of a 890 base pair (bp) primary pcr product. rnas from the nine vaccine strains of ahsv, and a number of a ...200919442836
a single-tube rt-pcr for rapid detection and differentiation of some african isolates of palyam serogroup orbiviruses.a single-tube nested reverse transcriptase (nrt) polymerase chain reaction (nrt-pcr) was developed and evaluated for detection of palyam serogroup orbiviruses ribonucleic acid (rna) in cell cultures and clinical samples. a pair of outer primers (pal1 and pal2), designed from genome segment three of chuzan virus of the palyam viruses serogroup, resulted in amplification of a primary 660-base pair (bp) pcr product. using a pair of internal (nested) primers (pal3 and pal4), the nrt-pcr produced a 3 ...200919500619
molecular and genetic comparisons of two serotypes of epizootic hemorrhagic disease of deer virus.the virus-specific double-stranded genome rna of 2 serotypes of epizootic hemorrhagic disease of deer virus (ehdv) was evaluated by use of coelectrophoresis in polyacrylamide and agarose gel systems. the molecular weights of virion rna segments were 0.32 to 2.57 x 10(6) for ehdv-1 and 0.33 to 2.54 x 10(6) for ehdv-2. seven of 10 double-stranded rna segments of the 2 serotypes had different electrophoretic mobilities in the polyacrylamide-gel electrophoresis system. although the individual rna se ...19863752679
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