Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| varietal differences in susceptibility to potato virus y. | 1946 | 20997935 | |
| the behaviour of some naturally occurring strains of potato virus y. | 1947 | 18901078 | |
| infectivity of aphids after several hours on tobacco infected with potato virus y. | 1953 | 13054709 | |
| ultra-violet irradiation of tobacco infected with potato virus y and the availability of virus to aphids. | 1954 | 13144758 | |
| evidence that potato virus y is carried near the tip of the stylets of the aphid vector myzus persicae (sulz.). | 1955 | 13270154 | |
| some effects of formaldehyde on potato virus y in vitro, and ability of aphids to transmit the virus when their stylets are treated with formaldehyde. | 1955 | 13270155 | |
| effects of depth of stylet penetration on aphid transmission of potato virus y. | 1956 | 13374600 | |
| titration of potato virus y by aphid transmission as affected by leaf development and supply of manganese. | 1958 | 13593189 | |
| variation in efficiency of aphid transmission of southern cucumber mosaic virus and potato virus y in pepper. | 1959 | 14446984 | |
| effect of tobacco mosaic virus and potato virus y on infection by potato virus x. | 1961 | 13776640 | |
| aphid transmission of potato virus y diminished by electrostatic charge. | 1961 | 13872331 | |
| different areas of tobacco leaves as sources of potato virus y for aphids. | 1962 | 13872333 | |
| topical susceptibility of tobacco leaves to potato virus y inoculated by aphids. | 1962 | 13872335 | |
| aphid transmission of potato virus y inhibited by oils. | 1962 | 14014835 | |
| chromatographic studies on plant viruses. 3. the purification of potato virus x, potato virus y, tobacco mosaic virus and potato stem mottle virus by chromatography on cellulose columns with polyethylene glycol-containing solutions as solvents. | 1964 | 14194134 | |
| increased hypersensitivity induced in tobacco by systemic infection by potato virus y. | 1968 | 5651028 | |
| intranuclear crystals and cytoplasmic membranous inclusions, associated with infection by two brazilian strains of potato virus y. | 1968 | 5698672 | |
| cross protection test with four strains of potato virus y in nicotiana tabacum l. cv. samsun. | 1969 | 5396065 | |
| partial purification of inclusions induced by tobacco etch virus and potato virus y. | 1971 | 4107552 | |
| on the size of the protein subunits in potato virus y. | 1972 | 5034362 | |
| location of the factor for resistance to potato virus y in tobacco. | 1973 | 4782667 | |
| enhancement by potato virus y of potato virus x synthesis in doubly infected tobacco depends on the timing of invasion by the viruses. | 1974 | 4821699 | |
| independent assembly of virions in tobacco doubly infected by potato virus x and potato virus y or tobacco mosaic virus. | 1974 | 4826211 | |
| a virus-induced component of plant sap needed when aphids acquire potato virus y from purified preparations. | 1974 | 4214349 | |
| potato virus y particles in plasmodesmata of tobacco leaf cells. | 1974 | 4813267 | |
| evidence that a component other than the virus particle is needed for aphid transmission of potato virus y. | 1974 | 4818682 | |
| characterization of the protein and nucleic acid of potato virus y strains isolated from pepper. | 1975 | 1114695 | |
| some characteristics of infectious rna from potato virus y. | 1975 | 1162905 | |
| [electron microscopic study of double infection induced by potato virus x and potato virus y in nicotiana glutinosa]. | 1975 | 1214639 | |
| assembly of flexuous plant viruses and their proteins. | recent experiments on the disassembly and assembly of some flexuous plant viruses and their proteins are described. the properties of reconstituted potato virus x and those of assembled potato virus y protein are considered as well as the suitability of other flexuous viruses for reconstitution studies. | 1976 | 13429 |
| [purification of pvy, pvx, tmv and spherical virus of pinks using polyethylene glycol and gel filtration]. | 1976 | 1012092 | |
| partial purification and characterization of the potato virus y helper component. | 1977 | 860406 | |
| [anomalous structures in the cytoplasm of cells infected with potato virus y]. | 1977 | 895578 | |
| antiphytoviral activity of 2,4 dioxo hexahydro triazine. | three treatments with 2,4 dioxo hexahydro triazine (dht) significantly reduced the concentration of potato virus x (pvx) in systemically infected tobacco plants. in hypersensitive plants dht caused a reduction in the number of local lesions produced by pvx. in systemic and hypersensitive hosts, treatment with dht resulted in a more or less marked reduction in the concentration of, and in the number of local lesions caused by, potato virus y (pvy), potato virus a (pva), tobacco mosaic virus (tmv) ... | 1979 | 42301 |
| towards a system for the identification and classification of potyviruses. ii. virus particle length, symptomatology, and cytopathology of six distinct viruses. | the mean particle lengths of lettuce mosaic virus (lmv), bean common mosaic virus (bcmv), passionfruit woodiness virus (pwv), potato virus y (pvy), sugarcane mosaic virus (scmv), and one strain of bean yellow mosaic virus (bymv) were all shown to be close to 750 nm. however, the length of two other serologically closely related strains of bymv, variants of pea mosaic virus referred to as pmv and spmv, had significantly longer particles, about 792 and 842 nm. each of the distinct viruses had read ... | 1981 | 18635068 |
| different helper factors associated with aphid transmission of some potyviruses. | myzus persicae transmitted watermelon mosaic virus (wmv) after acquiring it through artificial membranes from a solution of purified virus mixed with the soluble fraction from infected leaf extracts or by prefeeding on the soluble fraction before acquiring purified virus. wmv-induced helper factor assisted m. persicae in transmitting purified turnip mosaic virus (tumv) but not potato virus y (pvy). tumv-induced helper factor from infected leaves was ineffective for the transmission of purified w ... | 1981 | 18635077 |
| aphid alarm pheromone derivatives affecting settling and transmission of plant viruses. | 1,4-cycloaddition reactions between (e)-β-farnesene (the aphid alarm pheromone) and dienophiles, such as so2 and ro·co·c ≡ cco·or, gave products more stable and less volatile than the parent compound. in laboratory experiments, the numbers ofmyzus persicae (sulz.) settling and nymphs deposited on plants were decreased by some of the reaction products. acquisition, and thereby transmission, of the semipersistent beet yellows virus and the nonpersistent potato virus y, was decreased. | 1982 | 24414832 |
| the ultrastructure of nicotiana tabacum cells infected with potato virus x and potato virus y. | 1982 | 7143535 | |
| effect of ph and other conditions on immunosorbent electron microscopy of several plant viruses. | the efficiency of trapping, using immunosorbent electron microscopy, of particles of cucumber mosaic virus, lily symptomless virus, potato virus y, and carnation mottle virus, depended markedly on the ph of the virus extract. varying the ph of the serum had a smaller, though still significant effect. with potato virus y, the optimal ph of the virus extract varied with the host plant. it appears that for each virus and host a specific testing procedure is required to find the optimal trapping con ... | 1982 | 6752161 |
| helper components of two potyviruses are serologically distinct. | the specificity of antisera to helper component (hc) from tobacco vein mottling virus (tvmv)- or potato virus y (pvy)-infected tobacco plants was tested in immunoprecipitation and immunoabsorption chromatography experiments. treatment with the homologous antiserum abolished or drastically reduced the activity of either tvmv-hc or pvy-hc, as measured by their ability to effect aphid transmission of purified tobacco etch virus, while the heterologous antiserum had little or no effect on hc activit ... | 1983 | 18638913 |
| immunoprecipitation analysis of potyviral in vitro translation products using antisera to helper component of tobacco vein mottling virus and potato virus y. | the serological relationships of the products of in vitro translation of the rna of various potyviruses were analyzed by using antisera to helper component (hc) from tobacco plants infected with either tobacco vein mottling virus (tvmv) or potato virus y (pvy). the pvy-hc antiserum immunoprecipitated a specific pvy-rna translation product; this product was not reactive with antisera to pvy-induced cylindrical inclusion protein or capsid protein or to the two tobacco etch virus nuclear inclusion ... | 1984 | 18639815 |
| topographic analysis of tobacco etch virus capsid protein epitopes. | monoclonal antibodies have been prepared, which react with capsid protein of an aphid-transmitted isolate of tobacco etch virus (tev). ten different monoclonal antibodies were characterized with reference to (1) antibody class, (2) reactivity with different plant virus antigens, (3) the spatial relationship between epitopes, and (4) whether these epitopes were located on the exterior surface of the virion. three monoclonal antibodies were specific for tev isolates. these monoclonal antibodies re ... | 1985 | 18640514 |
| purification and characterization of potyvirus helper component. | helper component (hc) was purified from tobacco vein mottling (tvmv)- and potato virus y (pvy)- infected tobacco plants by sucrose gradient fractionation followed by affinity chromatography on oligo(dt)-cellulose and by gel electrophoresis. the subunit apparent molecular weights (m(r)) of the purified hcs were 53,000 (53k) and 58k for tvmv and pvy, respectively. antisera to these purified polypeptides specifically blocked the activity of the homologous hc, as determined by aphid transmission ass ... | 1985 | 18640517 |
| coat protein of potyviruses. 2. amino acid sequence of the coat protein of potato virus y. | the amino acid sequence of the coat protein of potato virus y (pvy), the type member of the potyvirus group, has been determined by protein sequencing techniques. the protein contains 267 amino acid residues with a calculated mol wt of 29,945. a comparison of the pvy coat protein sequence with those of tobacco etch virus (tev) and pepper mottle virus (pemv) predicted from nucleotide sequence data (r. f. allison, j. g. sorenson, m. e. kelly, f. b. armstrong, and w. g. dougherty, proc. natl. acad. ... | 1986 | 18640638 |
| the effect of helper component on the uptake and localization of potyviruses in myzus persicae. | 125i-labeled virions were used to determine whether helper component (hc) affected the uptake or distribution of potyviruses in aphids. aphids were allowed to acquire purified, 125i-labeled tobacco etch virus or potato virus y mixed with hc or with inactivated hc. helper component had no effect upon uptake of labeled virus, as measured by gamma counting. autoradiography of freeze-sectioned aphids revealed, however, that in the presence of hc, label was associated with the maxillary stylets and w ... | 1986 | 18640647 |
| on the inhibition of plant virus multiplication by ribavirin. | the inhibition of the replication of potato virus x (pvx), belladonna mottle virus, tobacco mosaic virus, potato virus y (pvy), and tobacco necrosis virus by ribavirin and pyrazofurin is described with emphasis on the inhibition of pvx by ribavirin. ribavirin inhibits an early step of pvx replication. the inhibition is reversed to different degrees by all ribo- and deoxyribonucleosides, most strongly by thymidine. in tobacco leaves, nucleosides compete with ribavirin for phosphorylation to monop ... | 1987 | 2821895 |
| coat protein of potyviruses. 4. comparison of biological properties, serological relationships, and coat protein amino acid sequences of four strains of potato virus y. | four strains of potato virus y, pvy-d, pvy-10, pvy-18, and pvy-43, obtained from different australian sources were compared on the basis of their biological, serological and coat protein structural properties. each of the strains could be distinguished on the basis of their reactions on selected test plant species. two of the pvy strains, pvy-d and pvy-10, induced symptoms similar to those produced by the pvyo strain group. the reactions of pvy-18 and pvy-43, although comparable to pvyn in some ... | 1988 | 3144263 |
| biotinylated nucleic acid hybridization probes for potato virus detection. | cdna libraries, representative of potato viruses x (pvxc strain) and y (pvy degrees strain) genomes were obtained. a pvx cdna cloned fragment was sequenced and biotinylated to be used as hybridization probe for the detection of purified virus or nucleic acid extracts of infected plants. dot hybridization assay was sensitive to detect 4 ng of viral particles, corresponding to about 200 pg of viral rna. the level of detection in infected plant extracts was as effective as that obtained with the el ... | 1988 | 3214274 |
| nucleotide sequence of the capsid protein gene of potato virus y (pvy). | the nucleotide sequence of the 3' terminal region of potato virus y (pvy) was determined. starting with a poly(a) tail of 18 residues a non-coding region of 335 nucleotides precedes the region encoding for the virus coat protein (cp) 801 nucleotides long ending with a tga. this region was located by comparing the predicted amino acid sequence with the one determined for the pvy capsid protein by shukla et al. (1). both sequences contained 267 amino acids sharing about 94% homology. they differ, ... | 1988 | 3245130 |
| resistance to potato leaf roll virus and potato virus y in somatic hybrids between dihaploid solanum tuberosum and s. brevidens. | many somatic fusion hybrids have been produced between a dihaploid potato solanum tuberosum and the sexually-incompatible wild species s. brevidens using both chemical and electrical fusion techniques. s. brevidens was resistant to both potato leaf roll virus (plrv) and potato virus y (pvy), the viruses being either at low (plrv) or undetectable (pvy) concentrations as determined by enzyme-linked immunosorbent assay (elisa). the s. tuberosum parent was susceptible to both viruses. a wide range o ... | 1988 | 24231991 |
| nucleotide sequence of the 3'-terminal region of potato virus yn rna. | the sequence of the 3'-terminal 1611 nucleotides of the genome of the tobacco veinal necrosis strain of potato virus y (pvyn) was determined. the sequence revealed an open reading frame of 1285 nucleotides, of which the start was not identified, and an untranslated region of 316 nucleotides upstream of a poly(a) tract. comparison of the open reading frame with the amino-terminal sequence of the viral coat protein enabled mapping of the start of the coat protein at amino acid -267, and indicated ... | 1989 | 2732687 |
| molecular cloning of a potato virus y genome: nucleotide sequence homology in non-coding regions of potyviruses. | an aphid-transmissible field isolate of potato virus y, strain mm, was purified from a pepper host and cloned. all but 18 terminal nucleotides of the 9.7 kb genome are apparently contained in two overlapping cdna clones. the library of cdna clones is likely to be representative of the viral rna population present in infected plants because restriction endonuclease maps derived from cloned and uncloned cdna are colinear, each region of the genome is represented by several independent clones, and ... | 1989 | 2769227 |
| nucleotide sequence of the coat protein gene of a necrotic strain of potato virus y from new zealand. | the sequence of the 3'-terminal 1,134 nucleotides of the genome of a new zealand isolate of a necrotic strain of potato virus y (pvyn) has been determined. this sequence contains one large open reading frame of 796 nucleotides, the start of which was not identified, which is capable of encoding a protein of 264 amino acid residues with a molecular weight of 29,631. comparison of the amino acid sequence with a published coat protein sequence of another strain, pvy-d, and with the amino acid seque ... | 1989 | 2802998 |
| cloning of the potato virus y genes encoding the capsid protein cp and the nuclear inclusion protein nib. | dna was synthesized complementary to the rna genome of potato virus y (pvy; strain go 16) and cloned into lambda vectors. the size of pvy-specific eco ri-restricted cdna ranged from 0.3 to approximately 22kb. two of the cdna clones each of which contained some 4kb of cdna sequence starting from the 3'-polyadenylated terminus were characterized by sequence analysis. presence of a single open reading frame suggests that pvy-specific proteins are synthesized as a polyprotein precursor. as with othe ... | 1989 | 2802999 |
| localization of virus-specific and group-specific epitopes of plant potyviruses by systematic immunochemical analysis of overlapping peptide fragments. | virus-specific or group-specific antibody probes to potyviruses can be produced by targeting the immune response to the virus-specific, n-terminal region of the capsid protein (29-95 amino acids depending on the virus) or to the conserved core region (216 amino acids) of the capsid protein, respectively. immunochemical analysis of overlapping, synthetic octapeptides covering the capsid protein of the johnsongrass strain of johnsongrass mosaic virus (jgmv-jg) has delineated the peptide sequences ... | 1989 | 2479020 |
| the use of peroxidase anti-peroxidase (pap) complexes in the detection of plant viruses by elisa. | an elisa test system for detection of plant viruses in field samples is described, based on the unlabelled antibody method using a peroxidase-antiperoxidase (pap) complex. novel features of the system include the use of acid-treated naked bacteria as combined carrier-adjuvants for the production of rabbit antiviral antibodies, and the use of acid-treated chicken antibodies (igy) for antigen trapping in the elisa. systems for detection of potato virus y (pvy), potato leafroll virus (plrv), grapev ... | 1989 | 2685004 |
| nucleotide sequence of potato virus y (n strain) genomic rna. | the complete nucleotide sequence of the genomic rna of the potyvirus potato virus y strain n (pvyn) was obtained from cloned cdnas. this sequence is 9704 nucleotides long and can encode a polyprotein of 3063 amino acids. the positions of the cleavage sites at the n terminus of the capsid and cytoplasmic inclusion proteins have been determined. other putative protein cleavage sites have been deduced by searching for consensus sequences and by analogy with the polyprotein of the tobacco vein mottl ... | 1989 | 2732709 |
| engineering resistance to mixed virus infection in a commercial potato cultivar: resistance to potato virus x and potato virus y in transgenic russet burbank. | potato virus x (pvx) and potato virus y (pvy) infection in potato may result in the loss of certification of seed potatoes and affect quality and yield of potatoes in commercial production. we transformed a major commercial cultivar of potato, russet burbank, with the coat protein genes of pvx and pvy. transgenic plants that expressed both cp genes were resistant to infection by pvx and pvy by mechanical inoculation. one line was also resistant when pvy was inoculated with viruliferous green pea ... | 1990 | 1366358 |
| the establishment of rat hybridoma cell lines secreting mcab against strains of potato virus y and analysis of its stability. | the rat splenocytes immunized with potato virus y (pvyn) and ratmyeloma (ir983) were fused by peg (m. w.1450). three kinds of stable hybridoma cell lines secreting specific monoclonal antibodies (mcabs) were derived. one kind of the cell lines producing mcabs reacts to pvyn specifically. another reacts to pvyo specifically. the third one reacts to both of the two strains. tested by the methods of sandwich-elisa and indirect-elisa, all kinds of mcabs did not react to seven plant viruses: tobacco ... | 1990 | 2104212 |
| nucleotide sequence of an altered virulence potato virus y coat protein gene (pvyh strain). | 1990 | 2251149 | |
| nucleotide sequence of the coat protein gene of pea seed-borne mosaic potyvirus. | the nucleotide sequence of a 1355 bp cdna representing the 3'-terminal sequences of pea seed-borne mosaic virus (psbmv) was determined. this sequence contained a single long open reading frame (orf) of 1189 bp ending with a single taa termination codon. downstream from the orf was an untranslatable region of 189 bp followed by eight bp of polyadenylate. the probable location of the psbmv coat protein codons within the long orf was determined by comparing the inferred amino acid sequence with oth ... | 1990 | 2391503 |
| polymerase chain-reaction-mediated cloning and expression of the coat protein gene of potato virus y in escherichia coli. | complementary dnas (cdnas) to the rna genome of a necrotic strain of potato virus y in japan (hokkaido univ. isolate of pvy-t:pvy-th) were synthesized and cloned into a plasmid pbr322. about 4.3 kbp of the cdna sequence containing the 3'-poly(a) tract of pvy-th was inserted into a recombinant plasmid pbryt88. the coat protein coding region (cp gene) in pbryt88 was amplified using the polymerase chain reaction (pcr) and subcloned into a plasmid puc119. the nucleotide sequence of the cp gene was d ... | 1990 | 2288070 |
| potato virus y helper component protein is associated with amorphous inclusions. | the distribution of the helper component (hc) protein of potato virus y in tissues and cells of infected plants was studied by immunoblotting and immunogold labelling techniques. this hc protein was found in leaf blade and vein tissue but not in the petiole of leaves. in infected cells, the protein was localized in rod-shaped cytoplasmic inclusions known as amorphous inclusions. | 1990 | 2230744 |
| comparative sequence of the helper component (hc) region of potato virus y and a hc-defective strain, potato virus c. | potato virus c (pvc), a non-aphid transmissible strain of potato virus y (pvy), was found to code for a protein (pvc-hc) which is similar in molecular weight and immunological reactivity to the helper component protein of pvy (pvy-hc). pvc-hc, however, was inactive with respect to its ability to effect aphid transmission of either pvc or pvy. the 5'-terminal 2.7-kb regions of pvc and pvy were sequenced. within the hc region there was 92% nucleotide homology between the two strains; comparison of ... | 1990 | 2219708 |
| cloning and mapping of the potato virus y genome and its in vitro expression. | full-length cdna of genomic rna of potato virus y (pvy) was cloned in one piece into a lambda vector. the order of the ecori and sali fragments of the inserted cdna was determined. this is the first report of the cloning of a long, expressible, potyvirus genome. the availability of such a clone is a prerequisite for any further study of the molecular biology of this group of viruses, as they are expressed into a self-processed primary polyprotein. | 1990 | 1980175 |
| the use of peptide-mediated electrofusion to select monoclonal antibodies directed against specific and homologous regions of the potyvirus coat protein. | whilst monoclonal antibodies (mab) to potyviruses have been generated, it has not been possible to produce molecules with high specificity or broad reactivity to defined conserved amino acid sequences. in the current study, peptide-mediated electrofusion was used to select for high efficiency antibody-secreting hybridomas after mice were immunized with highly immunogenic viral coat protein. mice were immunized with coat protein from either one potyvirus (potato virus y, pvy-d) or a mixture of fi ... | 1991 | 1719098 |
| comparison and differentiation of potyvirus isolates and identification of strain-, virus-, subgroup-specific and potyvirus group-common epitopes using monoclonal antibodies. | a panel of monoclonal antibodies (mabs) generated against an admixture of 12 potyvirus isolates was used to compare and differentiate diverse potyviruses. both native and denatured virions of strains of bean yellow mosaic (bymv), potato virus y, tobacco etch, pea seed-borne mosaic, iris severe mosaic, iris mild mosaic and asparagus virus-1 potyviruses were used as immunogen and as antigen for screening of the hybridoma cell lines. thirty cell lines secreting potyvirus-specific antibodies reactiv ... | 1991 | 1703561 |
| resistance in solanum brevidens to both potato virus y and potato virus x may be associated with slow cell-to-cell spread. | a series of experiments was carried out to investigate the nature of the resistance of the wild potato species, solanum brevidens, to potato virus x (pvx) and potato virus y (pvy). in vitro inoculation of leaf protoplasts of s. brevidens and the virus-susceptible dihaploid s. tuberosum genotype pdh40 with pvx or pvy using polyethylene glycol showed that protoplasts of both species were similar in susceptibility. however, examination of protoplasts prepared from the leaves of s. tuberosum and s. ... | 1991 | 1993865 |
| development and application of a nonradioactive nucleic acid hybridization system for simultaneous detection of four potato pathogens. | cdna clones of potato virus x (pvxcp strain), potato virus y (pvyo strain), potato leaf roll virus (plrv) and potato spindle tuber viroid (pstv) were used separately or combined for the detection of the corresponding rnas in extracts of infected plants. a general method for the rapid preparation of rna extracts without use of organic solvents (i.e. phenol) was developed for this purpose. plant extracts from a range of field, artificially inoculated germplasm genotypes, micro-propagated and proto ... | 1991 | 2016393 |
| replication of potato virus x rna is altered in coinfections with potato virus y. | potato virus x (pvx) and potato virus y (pvy) may coinfect tobacco to cause a classic synergistic disease. in the acute stage the disease is characterized by a dramatic increase in the accumulation of infectious pvx particles, with no corresponding increase or decrease in the accumulation of pvy. the accumulation of pvx genomic rna and coat protein has been examined in doubly versus singly infected tobacco leaves. these experiments indicate that the levels of both viral components increase in do ... | 1991 | 2024486 |
| [the antigenic characteristics of the capsid proteins in strains of the potato virus y]. | the antigenic properties of capsid proteins of potato virus y (pvy) strains have been studied, the most wide antigenic specificity of necrotic group strains has been marked. several antigen-active strains of necrotic group strains (pvy-n far east, pvy-n leningrad, pvy-n moscow) and common group (pvy-o-3 moscow, pvy-o far east) have been revealed. antisera against these strains reacted with any pvy strain. virus specific surface epitope, corresponding to position 198-208 of polypeptide chain has ... | 1991 | 1722712 |
| the complete nucleotide sequence of pea seed-borne mosaic virus rna. | the complete nucleotide sequence of the rna genome of pea seed-borne mosaic virus (psbmv) was determined from cloned cdna and by direct sequencing of viral rna. the psbmv genomic sequence was determined to be 9924 nucleotides in length excluding the poly(a) tract. the rna contained an open reading frame (orf) of 9618 nucleotides with the potential to encode a polyprotein with a calculated mr of 364000 (364k). the orf was flanked by a 5' untranslated leader sequence of 143 nucleotides and a 3' un ... | 1991 | 1940858 |
| protection against detrimental effects of potyvirus infection in transgenic tobacco plants expressing the papaya ringspot virus coat protein gene. | we obtained transgenic tobacco plants expressing the papaya ringspot virus (prv) coat protein (cp) gene by transformation via agrobacterium tumefaciens. expression was effectively monitored by enzyme-linked immunosorbent assays (elisa) of crude tissue extracts. subcloned plants derived from eight original ro transformants were inoculated with potyviruses: tobacco etch (tev), potato virus y (pvy), and pepper mottle (pemv). plants that accumulated detectable levels of the prv cp showed significant ... | 1991 | 1367635 |
| characterization of a gametoclonal variant controlling virus resistance in tobacco. | potato virus y (pvy), susceptible tobacco (nicotiana tabacum l.) cultivar, mcnair 944, was subjected to in vitro anther culture to determine if genetic variability for virus resistance could be induced among resulting haploids. five hundred and forty-five haploids were produced and inoculated with a highly necrotic strain (nn) of pvy. one haploid plant survived, even though it was infected with the virus. selfed progenies of a chromosome-doubled plant of this variant, designated nc 602, proved t ... | 1991 | 24221151 |
| agrobacterium-mediated transformation of solanum tuberosum l. cv. 'russet burbank'. | stem sections from shoot cultures maintained in vitro were used to produce transgenic plants of the potato, solanum tuberosum l. cv. 'russet burbank'. stem internode pieces inoculated with agrobacterium tumefaciens containing coat protein genes from potato virus x and potato virus y, produced shoots with a frequency of 60% in the absence of selection and 10% on medium containing 100 mg/l kanamycin monosulfate. regenerated shoots were assayed for kanamycin resistance by placing stem segments on c ... | 1991 | 24226160 |
| the complete nucleotide sequence of pepper mottle virus genomic rna: comparison of the encoded polyprotein with those of other sequenced potyviruses. | the complete nucleotide sequence of a pepper mottle virus isolate from california (pepmov c) has been determined from cloned viral cdnas. the pepmov c genomic rna is 9640 nucleotides excluding the poly(a) tail and contains a long open reading frame starting at nucleotide 168 and potentially encoding a polyprotein of 3068 amino acids. comparison of the pepmov c presumptive polyprotein with those of other sequenced members of the potyvirus group, including tobacco etch virus (tev), tobacco vein mo ... | 1992 | 1413501 |
| nucleotide sequence of the 3'-terminal region of potato virus a rna. | the sequence of the 3'-terminal region of the genome of the potato virus a (pva) was obtained from two independent cdna clones. this sequence is 1383 nucleotides long and contains an open reading frame of 1178 nucleotides, ending with the translation termination codon taa and followed by untranslated region of 205 nucleotides. since the n-terminal amino acid of the coat protein of pva was blocked, the position of the putative coat protein cleavage site has been deduced by searching for consensus ... | 1992 | 1604933 |
| cloning and sequencing of the 3' half of a potato virus y (o strain) genome encoding the 5k protein, protease, polymerase and coat protein. | 1992 | 1630927 | |
| effects of antisense oligodeoxynucleotide hybridization on in vitro translation of potato virus y rna. | potato virus y (pvy), a potyvirus, has an rna genome containing 9704 nucleotides of which 185 belong to the 5' nontranslated region (ntr). contrary to most eukaryotic mrnas that have a cap structure, the potyvirus rna has a genome-linked protein (vpg). in order to understand the mechanisms of pvy rna translation initiation, hybrid-arrest translation was used to localize sequences involved in binding of proteins and/or ribosomes. the 5' ntr was fused to the beta-glucuronidase (gus) reporter gene. ... | 1992 | 1549909 |
| coat protein properties suggest that azuki bean mosaic virus, blackeye cowpea mosaic virus, peanut stripe virus, and three isolates from soybean are all strains of the same potyvirus. | the interrelationship of a number of potyviruses infecting legumes has been investigated by comparing molecular properties of their coat proteins. comparison of the coat proteins by the techniques of amino acid analysis and page was inadequate to distinguish strains from distinct potyviruses. however, high-performance liquid chromatographic peptide profiles of tryptic digests of coat proteins of these legume-infecting potyviruses enabled such assignments to be made. these data indicate that amin ... | 1992 | 1500273 |
| production and characteristics of strain common antibodies against a synthetic polypeptide corresponding to the c-terminal region of potato virus y coat protein. | comparing the predicted amino acid sequence between two japanese potato virus y (pvy) strains, necrotic strain and ordinary strain, it was found that the c-terminal regions (h2n-httedvspsmhtllgvknm-cooh) of the coat proteins in the two strains were completely conserved. the conserved amino acid sequence was also found in the c-terminal region coat protein of pvy-36, a strain which did not react with monoclonal antibodies specific to the necrotic and the ordinary strain respectively. antibodies w ... | 1992 | 1474133 |
| factors influencing immune electron microscopy of flexuous potato viruses. | effects of ph of extraction buffers, ph and titer of trapping antisera and their combinations, virus acquisition time and virus host on the trapping efficiency of flexuous potato viruses x, s and y (pvx, pvs and pvy) in immune electron microscopy were evaluated. addition of ethylene-diamine-tetraacetic acid to the extraction buffer improved trapping of pvy, adversely affected pvs but not pvx. combinations of antisera had differential adverse effect on trapping which was maximum with the mixture ... | 1992 | 1364019 |
| establishment of hybridoma cell line secreting specific monoclonal antibodies against turnip mosaic virus and analysis of properties of the mcab. | monoclonal antibodies (mcab) of hybridomas derived from the fusing of the mouse splenocytes immunized by tumv with balb/c mouse myeloma cells (sp2/0-ag14). five kinds of hybridoma cell line were produced by indirect-elisa screening and cloning three times with limiting dilution. four kinds of hybridoma produced antibodies respectively reactive to tumv c1, c3, c4 and c5. one kind was reactive to all five strains of tumv. in indirect-elisa and sandwich-elisa tests, tumv specific monoclonal antibod ... | 1992 | 1343828 |
| evidence for sense rna-mediated protection to pvyn in tobacco plants transformed with the viral coat protein cistron. | the coat protein (cp) cistron of the tobacco veinal necrosis strain of potato virus y (pvyn), supplemented with translational start signals, was cloned into an agrobacterium tumefaciens ti transformation vector. transformation of tobacco leaf discs resulted in 99 transgenic lines which were subsequently analysed for the presence and expression, at both the transcriptional and translational level, of the cp-gene. although cp-specific rna transcripts were produced in all plants no cp could be dete ... | 1992 | 1280473 |
| evidence that pepper mottle virus and potato virus y are distinct viruses: analyses of the coat protein and 3' untranslated sequence of a california isolate of pepper mottle virus. | pepper mottle virus (pepmov) is a member of the large and complex genus potyvirus, and is classically distinguished from other members of the genus by differential host range and cytopathology as well as serology of the coat protein and cytoplasmic inclusion body proteins. here we report the deduced amino acid sequence of the coat protein of a california potyvirus identified by a variety of classical methods as pepmov (pepmov c). comparison of the 3' untranslated nucleic acid sequence and the de ... | 1992 | 1450760 |
| is pepper mottle virus a strain of potato virus y? | on the basis of serological properties and host plant reactions pepper mottle virus (pepmov) has been classified as a potyvirus related to, but distinct from, other pepper-infecting potyviruses, potato virus y (pvy) and tobacco etch virus (tev). recent amino acid and nucleotide sequence data show that pepmov is more closely related to pvy than previously assumed. pepmov shows a high degree of homology to various pvy strains in both the coat protein and the 3' non-translated sequences, while unre ... | 1992 | 1450759 |
| a comparison of pepper mottle virus with potato virus y and evidence for their distinction. | pepper mottle virus (pepmov) was identified as a distinct potyvirus infecting peppers in arizona and florida in the 1970's. the distinction of pepmov from potato virus y (pvy) has recently been challenged on the basis of sequence comparisons of the coat proteins and of the 3' nontranslated regions of the viral rnas. we summarize the biological, cytological, serological, and in vitro translational studies which compare the apparent differences, and also similarities, between pepmov and pvy. we co ... | 1992 | 1450758 |
| cleavage of potato virus y polyprotein in escherichia coli depending on the proteolytic activity of viral protease. | we have cloned a 5-kb cdna corresponding to the 3'-half of genomic rna of potato virus y (pvy), the type member of plant potyvirus. based on its nucleotide sequence, the cdna was presumed to encode pvy protease responsible for the self-processing of polyprotein precursor expressed from pvy genome. to test its proteolytic activity, the cdna was modified so as to express the protease-polymerase-coat protein (cp) portion of pvy polyprotein in escherichia coli. by western blotting analysis of the ce ... | 1993 | 7763774 |
| coat protein gene-mediated resistance to potato virus y in tobacco: examination of the resistance mechanisms--is the transgenic coat protein required for protection? | we transformed sr1 tobacco with a cdna fragment of the potato virus y strain n (pvyn) 605 genome which contained the 3' end of the nib polymerase gene, the coat protein (cp) coding sequence, and most of the viral 3' untranslated region. complete resistance to several pvy strains was obtained in plants of the r2 generation. the resistance to pvyn and pvyo strains was maintained when the plants were inoculated dually with the potato potyviruses v or a (pvv or pva). only partial resistance to pvyn ... | 1993 | 8324247 |
| characterization of antigenic epitopes of potato virus y. | immunochemical analysis of overlapping synthetic hexapeptides covering the entire length of the coat protein of potato virus y (pvy) revealed immunodominant regions both at the n-terminal and at the c-terminal end of the coat protein. immunization of rabbits with synthetic peptides representing n- and c-terminal regions of the coat protein resulted in production of antibodies that reacted with pvy. antigenicity of pvy peptides was found to correlate with predicted beta turns, with hydrophilicity ... | 1993 | 7680576 |
| efficient pathogen-derived resistance induced by integrated potato virus y coat protein gene in tobacco. | the coat protein (cp) gene from potato virus y (hungarian isolate, pvy-h) was engineered into agrobacterium tumefaciens binary vector for expression in different tobacco lines. three different nicotiana tabacum breeding lines were transformed and the integration of the cp gene was confirmed by pcr technique using genomic dna preparations. the transcription and expression of the integrated cp gene was detected by northern and western blots. pathogen-derived resistance was demonstrated by inoculat ... | 1993 | 8268262 |
| mutational analysis of the amino acid residues essential for the cis and trans cleavage activity of the potato virus y 50-kda protease. | to examine the proteolytic activities of various truncated derivatives of the potato virus y (pvy) 50-kda protease, the derivatives were expressed in escherichia coli in polyprotein forms fused with coat protein (cp). for the intermolecular cleavage reaction, the truncated proteases were expressed together with the substrate protein containing the polymerase-cp junction. the activity was evaluated by the amount of the mature cp released from the precursor by the intra- and intermolecular cleavag ... | 1993 | 7764224 |
| the 5' untranslated region of pvy rna, even located in an internal position, enables initiation of translation. | potato virus y (pvy) is the type member of the potyvirus group. potyviruses, like picorna-, como-, and nepoviruses, belong to the picornavirus-like supergroup. all these viral rnas have a vpg at their 5' end, and for four picornaviruses and one comovirus internal initiation of translation has been reported. to know if such a translational mechanism holds true for potyviral rnas, the 5' nontranslated region (ntr) of pvy rna was placed in an internal position, either by adding 91 bases upstream of ... | 1993 | 8122396 |
| mutations in zucchini yellow mosaic virus helper component protein associated with loss of aphid transmissibility. | zucchini yellow mosaic virus (zymv) is a potyvirus transmitted by aphids in a non-persistent manner. isolates having partially or totally lost their ability to be transmitted by aphids have been isolated and found to be affected in their helper component activities. we have sequenced the helper component coding region of poorly aphid-transmissible (pat) variants of two strains of zymv, e15 and r5a. mutations have been identified at the nucleotide level leading to two amino acid changes in the e1 ... | 1993 | 8277279 |
| genetic engineering of potyvirus resistance using constructs derived from the zucchini yellow mosaic virus coat protein gene. | three versions of the zucchini yellow mosaic virus (zymv) coat protein gene were engineered for expression in plants: the full-length coat protein sequence, the conserved core portion of the gene, and an antisense version. these constructs were introduced into muskmelon (cucumis melo) and tobacco plants (nicotiana tabacum) via agrobacterium tumefaciens-mediated transformation; gene expression was verified by northern and western analysis. transgenic r0 and r1 muskmelon plants expressing the full ... | 1993 | 8324251 |
| signal for potyvirus-dependent aphid transmission of potato aucuba mosaic virus and the effect of its transfer to potato virus x. | a british isolate of potato aucuba mosaic potexvirus (pamv) was transmitted by aphids (myzus persicae) which had fed previously on a source of potato y potyvirus (pvy). nucleotide sequence analysis of the pamv coat protein gene indicated that amino acid residues 14 to 16 from the n terminus of the coat protein have the sequence dag, which is also found in the coat proteins of potyviruses and is required for their aphid transmissibility. a recombinant virus isolate (txpa7) was produced in which a ... | 1993 | 8336116 |
| taxonomic relationships between distinct potato virus y isolates based on detailed comparisons of the viral coat proteins and 3'-nontranslated regions. | detailed comparisons were made of the sequences of the coat protein (cp) cistrons and 3'-nontranslated regions (3'-ntr) of 21 (geographically) distinct isolates of potato virus y (pvy) and a virus isolate initially described as pepper mottle virus (pepmov). multiple sequence alignments and phylogenetic relationships based on these alignments resulted into a subgrouping of virus isolates which largely corresponded with the historical strain differentiation based on biological criteria as host ran ... | 1993 | 8347079 |
| nucleotide sequence of the capsid protein cistrons from six potato virus y (pvy) isolates infecting tobacco. | complementary dna libraries representing the capsid protein cistron of the potato virus y (pvy) isolate 'chilean', 'hungarian', msnr, nsnr, o, and 'potato us' were synthesized and used as template for polymerase chain reaction (pcr) amplification. an aug codon for initiating a discrete capsid protein (cp) open reading frame was embedded upstream of the first codon of the cp cistrons. pcr-amplified products of the expected size of 0.8 kilo bases were cloned into the transcription vector pbs(+). t ... | 1993 | 8352655 |
| plants transformed with a cistron of a potato virus y protease (nia) are resistant to virus infection. | an oligonucleotide carrying signals for translation initiation in plants was engineered upstream to a cdna clone containing nucleotides 5812-7260 of the potato virus y (pvy) genome. this fragment contains all but the first 100 5' terminal bases of the cistron encoding one of the pvy proteases (nia) as well as the first 251 bases of the next cistron (nib). nicotiana tabacum cv. sr1 plants were transformed with this fragment. the presence of the nia sequences in transformed plants was determined b ... | 1993 | 8356047 |
| expression of the "helper component" protein of potato virus y (pvy) in e. coli: possible involvement of a third protease. | transmission of potyviruses by aphids depends on the presence of a virus encoded helper-component protein (hc) that also exhibits protease activity. hc was expressed in e. coli from two types of clones: a full-length cdna clone of pvy and two 5' end clones containing the first three cistrons (3.6-3.7 kbp). the clones derived from the 5' end of pvy expressed hc of the size of the mature component. other proteins reacting with antibodies to hc were also observed, and their sizes corresponded with ... | 1993 | 8367942 |