Publications

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separation of foot-and-mouth disease virus leader protein activities; identification of mutants that retain efficient self-processing activity but poorly induce eif4g cleavage.foot-and-mouth disease virus is a picornavirus and its rna genome encodes a large polyprotein. the n-terminal part of this polyprotein is the leader protein, a cysteine protease, termed lpro. the virus causes the rapid inhibition of host cell cap-dependent protein synthesis within infected cells. this results from the lpro-dependent cleavage of the cellular translation initiation factor eif4g. lpro also releases itself from the virus capsid precursor by cleaving the l/p1 junction. using site-dir ...201728452293
simple, quick and cost-efficient: a universal rt-pcr and sequencing strategy for genomic characterisation of foot-and-mouth disease viruses.foot-and-mouth disease (fmd) is a major contributor to poverty and food insecurity in africa and asia, and it is one of the biggest threats to agriculture in highly developed countries. as fmd is extremely contagious, strategies for its prevention, early detection, and the immediate characterisation of outbreak strains are of great importance. the generation of whole-genome sequences enables phylogenetic characterisation, the epidemiological tracing of virus transmission pathways and is supporti ...201728442328
evaluation of immune responses of stabilised sat2 antigens of foot-and-mouth disease in cattle.foot-and-mouth disease (fmd) vaccines with improved stability and less reliant on a cold-chain are needed to improve the longevity of immune responses elicited in animals. this is especially so for serotypes o and sat2 which are unstable in mildly acidic ph conditions or at elevated temperatures leading to dissociation of the capsid (146s particle) and loss of immunogenicity. previously, stabilised sat2 viruses were generated by reverse genetic approaches and assessed in vitro and in vivo with a ...201728431813
recombinant foot-and-mouth disease virus (fmdv) non-structural protein 3a fused to enhanced green fluorescent protein (egfp) as a candidate probe to identify fmdv-infected cattle in serosurveys.recombinant protein 3a-egfp, a fusion construct between foot-and-mouth disease virus (fmdv) non-structural protein 3a and the enhanced green fluorescent protein (egfp) was expressed in bl21-de3 cells. the identity of the partially purified protein 3a-egfp was confirmed by its reactivity with sera from cattle infected with fmdv and with a monoclonal antibody specific for fmdv-3abc (mab3h7) in western blot assays. no reactivity was observed with sera from uninfected vaccinated animals. the perform ...201728421368
detection of genome, antigen, and antibodies in oral fluids from pigs infected with foot-and-mouth disease virus.virus nucleic acids and antibody response to pathogens can be measured using swine oral fluids (ofs). detection of foot-and-mouth disease virus (fmdv) genome in swine ofs has previously been demonstrated. virus isolation and viral antigen detection are additional confirmatory assays for diagnosing fmdv, but these methods have not been evaluated using swine of. the objectives of this study were to further validate the molecular detection of fmdv in oral fluids, evaluate antigen detection and fmdv ...201728408775
foot-and-mouth disease virus infection inhibits lgp2 protein expression to exaggerate inflammatory response and promote viral replication.the role of the innate immune protein lgp2 (laboratory of genetics and physiology 2) in fmdv-infected cells remains unknown. here, we demonstrate the antiviral role of lgp2 during fmdv infection. fmdv infection triggered lgp2 mrna expression but reduced protein expression. overexpression of lgp2 suppressed fmdv replication, and the inflammatory response was significantly inhibited by lgp2 in virus-infected cells. the n-terminal dexdc and the c-terminal regulatory domain regions of lgp2 were esse ...201728406479
phylodynamics of foot-and-mouth disease virus o/panasia in vietnam 2010-2014.foot-and-mouth disease virus (fmdv) is endemic in vietnam, a country that plays an important role in livestock trade within southeast asia. the large populations of fmdv-susceptible species in vietnam are important components of food production and of the national livelihood. in this study, we investigated the phylogeny of fmdv o/panasia in vietnam, reconstructing the virus' ancestral host species (pig, cattle or buffalo), clinical stage (subclinical carrier or clinically affected) and geographi ...201728403902
efficacy of a high potency o1 manisa foot-and-mouth disease vaccine in cattle against heterologous challenge with a field virus from the o/me-sa/ind-2001 lineage collected in north africa.outbreaks of foot-and-mouth disease (fmd) in north africa (2013) and the gulf states (2013) of the middle east have been caused by a fmd viral lineage (o/me-sa/ind-2001) that was before 2013 restricted to the indian sub-continent. this study was undertaken to assess the in vivo efficacy of a fmd virus emergency vaccine type o1 manisa against heterologous challenge with a representative field virus (o/alg/3/2014) from this emerging lineage. this widely available vaccine was selected since in vitr ...201728396208
preclinical diagnosis: predicting its ability to improve control of farm-to-farm foot-and-mouth disease transmission in cattle.foot-and-mouth disease (fmd) can cause large disruptive epidemics in livestock. current eradication measures rely on the rapid clinical detection and removal of infected herds. here, we have evaluated the potential for preclinical diagnosis during reactive surveillance to reduce the risk of between farm transmission. we used data from transmission experiments in cattle where both individual animal samples, such as blood, probang, saliva or nasal swabs, and herd level samples, such as air samples ...201728330886
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