Publications

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haemagglutination by type sat 2 foot-and-mouth disease viruses. 19734353214
[comparison between dna synthesized by enzyme fractions extracted from foot-and-mouth disease virus and from atypical rat epithelioma cells]. 19734351685
evidence for a group protein in foot-and-mouth disease virus particles. 19734353213
[immunological aspects of foot-and-mouth disease virus (author's transl)]. 19734377405
[accumulation of specific foot-and-mouth disease virus antibodies in the ascitic fluid of white rats]. 19734362264
[the effect of interferon and guanidine on the inactivation of rna of foot-and-mouth disease virus infected cells]. 19734373360
[the density of the foot-and-mouth disease virus in cscl solutions]. 19734365551
immunological activity of foot-and-mouth disease virus purified by polyethylene glycol precipitation. 19734362322
studies of the foot-and-mouth disease virus sub-types using antigens inactivated by gamma radiations. 19734362323
[study of the foot-and-mouth disease virus cultured on the bhk-21 cell line]. 19734370848
the effect of rifampicin, actinomycin d and mitomycin c on poliovirus and foot-and-mouth disease virus replication. 19734357749
the influence of relative humidity on the stability of foot-and-mouth disease virus in aerosols from milk and faecal slurry. 19734360318
foot-and-mouth disease virus: protection induced in infected mice by two orally-administered interferon inducers. brief report. 19734749257
observations of cattle, goats and pigs after administration of synthetic interferon inducers and subsequent exposure to foot and mouth disease virus.polyriboinosinic-polyribocytidylic acid (poly [ri.rc]) was administered intravenously to 11 cattle and 13 goats in doses of 0.25 to 4.0, and 1.0 to 5.0 mg/kg, respectively. subsequent exposure of these and untreated control animals to foot and mouth disease virus (fmdv) failed to demonstrate any differences in either the course or severity of the disease. serum interferon was detected in cattle one hour after the intravenous administration of poly (ri.rc). six pigs given 4, 20, or 100 mg/kg of i ...19734356316
serum immunoglobulins in white rats immunized with foot-and-mouth disease virus antigens. 19734148114
[differences in the antigenic structure of strains of the foot-and-mouth disease virus]. 19744143073
antigenic variation of venezuelan strains of foot and mouth disease viruses. 19744143492
[demonstration of antibody-synthesizing cells in mice and swine by means of local hemolysis in gel following immunization with foot-and-mouth disease virus and vaccine]. 19744154623
metabolic studies on heart of unweaned rabbits infected with foot-and mouth disease virus. 19744154715
[detection of antibody-forming cells by means of local hemolysis in gel (lhg) in mice and pigs after immunization with infectious or inactivated foot-and-mouth disease virus (author's transl)]. 19744143236
application of immuno-peroxidase techniques for the detection of foot-and mouth disease virus antigens. 19744377950
[foot-and-mouth disease in ethiopia. distribution of serotypes of foot-and-mouth disease virus]. 19744377719
the foot-and-mouth disease virus subtype variants in kenya.the subtype variants found in kenya in the past ten years have been studied. the type o and type sat 2 subtypes have a distinct geographical distribution which appears to be associated with livestock movement patterns. the type a viruses have a greater tendency to antigenic variation and their geographical distribution is less distinct. in type c only minor differences exist between the three viruses studied.19744371008
translation products of foot-and-mouth disease virus-infected baby hamster kidney cells. 19744371593
dependence of rna replication on continuous protein synthesis in a temperature-sensitive mutant of foot-and-mouth disease virus. 19744372312
chromatographic preparation of purified structural proteins from foot-and-mouth disease virus. 19744365645
[isolation of foot-and-mouth-disease virus from organs of infected, slaughtered sheep]. 19744372987
differentiation of type a foot-and-mouth disease virus subtypes by double- and radial-immunodiffusion analysis. 19744368590
a study of foot-and-mouth disease virus strains by complement fixation. i. a model for the fixation of complement by antigen-antibody mixtures.an examination was made of the relations between antigen, antibody and fixation of complement with foot-and-mouth disease virus (fmdv). it was found that complement fixation in this system follows the same principles as models developed in other antigen/antibody systems. the assumption that there is a relation of direct proportionality between the amount of complement fixed and the amount of antiserum reacting with constant antigen was found to be incorrect. an alternative method was proposed fo ...19744367223
[cell susceptibility to fmdv by cell monolayers previously exposed to inactivated sendai virus (author's transl)]. 19744374909
[effect of insecticides on metabolism and susceptibility of ib-rs-2 cells to foot-and-mouth disease virus (author's transl)]. 19744374910
neutralizing activity in the serum and oesophageal-pharyngeal fluid of cattle after exposure to foot-and-mouth disease virus and subsequent re-exposure. 19744365187
sodium dodecylsulfate-dependent anomalies in gel electrophoresis: alterations in the banding patterns of foot-and-mouth disease virus polypeptides. 19744363849
proceedings: the immunizing potency of foot-and-mouth disease virus vaccines for swines. 19744364055
studies with foot-and-mouth disease virus in british deer (red, fallow and roe). 19744369242
[isolation and study of foot and mouth disease virus type a during the epizootic of 1973]. 19744377474
studies with foot-and-mouth disease virus in british deer (red, fallow and roe). ii. recovery of virus and serological response. 19744369243
requirement for double-stranded rna during the in vitro synthesis of rna by foot-and-mouth disease virus replicase. 19744370339
inhibition of foot-and-mouth disease virus replicase by frog virus 3 particles. 19744367303
growth of foot-and-mouth disease virus in the different layers of bovine omasum in suspended cultures.when suspended cultures of bovine omasum were cultured without agitation, the epithelium soon degenerated and foot-and-mouth disease virus multiplied mainly in the corium cells. five days of preincubation were needed to reach a population of corium cells that could yield virus at a titer of 10(6.70) to 10(6.95) mean tissue culture infective doses per ml. the virus was freely released from the cells into the medium only when the degenerated epithelium was removed from the subepithelial tissue pri ...19744375435
the detection of foot-and mouth disease virus antigens in infected cell cultures by immuno-peroxidase techniques. 19744362406
a simple method for the quantification of 140s particles of foot-and-mouth disease virus (fmdv). 19744374160
isolation of the coat proteins of foot-and-mouth disease virus and analysis of the composition and n-terminal endgroups. 19744374202
inhibitors of foot-and-mouth disease virus. i. effect of edta, temperature, and interferon on the viral growth cycle. 19744375450
[role of amino acids in the formaldehyde inactivation of the foot-and-mouth disease virus]. 19744377336
foot-and-mouth disease virus: plaque reduction neutralization tests. 19744365185
comparative electrophoretic studies of foot-and-mouth disease virus proteins. 19744360391
the effect of repeated vaccination in an enzootic foot-and-mouth disease area on the incidence of virus carrier cattle.a comparison was made of the incidence of foot-and-mouth disease virus ;carrier' cattle in an unvaccinated enzootic area and an area where routine 6-monthly vaccination with an inactivated vaccine had been carried out for 3-4 years. the incidence of carriers in the vaccinated area was 0.49% as compared to 3.34% in the non-vaccinated area. the results indicate that, provided the immune status of the vaccinated herd is maintained at a level sufficient to prevent outbreaks of clinical disease and t ...19744370898
association of foot-and-mouth disease virus replicase with rna template and cytoplasmic membranes. 19744364882
the survival of foot-and-mouth disease virus in african buffalo with non-transference of infection to domestic cattle. 19744364599
a study of foot-and-mouth disease virus strains by complement fixation. ii. a comparison of tube and microplate tests for the differentiation of strains.several foot-and-mouth disease virus strains were examined by complement-fixation tests in microplates and in tubes. it was established that the two systems are comparable, although greater reproducibility is obtained with tube tests. while microplate tests are a satisfactory method for the differentiation of strains, tube tests provide a more precise method for the identification of small antigenic differences.19744367224
[cultivation of the foot-and-mouth disease virus in continuosly inoculated cells of piglet kidney]. 1975163519
[setting up a neutralization reaction for the foot-and-mouth disease virus]. 1975163521
[complement fixation micromethod for determining the type of foot-and-mouth disease virus]. 1975163522
removal of acetylethyleneimine from suspensions of inactivated foot-and-mouth disease virus. 1975166252
[influence of varying storage temperatures on the infectivity and antigenicity of foot-and-mouth disease virus strains before and after inactivation]. 1975166530
the sub-type classification of strains of foot-and-mouth disease virus.sixteen foot-and-mouth disease virus (fmdv) strains of type sat 1 were compared in complement-fixation tests. with the test used, the range of antigenic variation within a type appeared to be greater than previously described. the concept of a sub-type group within which all strains are more closely related to each other than to any strain outside the group was not supported. considering the group of strains studied, it is suggested that the classification of strains is best achieved by moninati ...1975164501
a comparison of some immunological methods for the differentiation of strains of foot-and-mouth disease virus.two fmdv strains which had been previously differentiated by complement-fixation were compared by guinea-pig protection test, kinetic neutralization and micro-neutralization tests. it was found that these tests, which have not been previously applied by the methods described, were all capable of fmdv strain differentiation. similar differences were found by all methods, which suggests that comparisons made by cross-cf, cross-neutralization or cross-protection involve measurement of the same anti ...1975164500
production, isolation, and partial characterization of three foot-and-mouth disease virus temperature-sensitive mutants.three high temperature-sensitive (ts) mutants of foot-and-mouth disease virus were characterized by their relative abilities to grow at 33 or 38.5 c, to kill infant mice, to infect guinea pigs, and to produce foot-and-mouth disease in steers. mutants ts-24 and ts-42 did not grow at 38.5 c; both may have produced considerable quantities of noninfectious virus particles at 33 c. a third mutant, ts-22, appeared "leaky" because it multiplied to a limited extent during prolonged incubation at the non ...1975166915
studies with foot-and-mouth disease virus in british deer (muntjac and sika). clinical disease, recovery of virus and serological response. 1975167058
some investigations on the adjuvant mechanism of deae dextran.in vitro it was shown that adsorption of inactivated fmdv onto deae-d kieselgur columns did not occur in the presence of 0.1--0.15m nacl. these nacl concentrations are present in deae-d/fmdv vaccines and in the tissues of animals. therefore, adsorption of virus antigen does not appear to be responsible for the adjuvant effect of deae-d. in pigs it was demonstrated that deae-d exerts its optimal adjuvant effect, as measured by the formation of neutralizing antibodies and protection against challe ...1975164161
the use of the haemagglutination-inhibition test for detecting antibodies to type sat 2 foot-and-mouth disease viruses in cattle sera.two strains of type sat 2-foot-and-mouth disease virus which gave high titres of haemagglutinin activity reacted type-specifically in direct haemagglutination-inhibition tests with reference, bovine convalescent antisera. comparisons of the haemagglutination-inhibition and the serum neutralization tests using cattle sera showed that both were equally specific and sensitive for detecting virus antibody.1975163274
cross-reactions of normal bovine serums to foot-and-mouth disease virus in plaque-reduction neutralization and radial immunodiffusion.serums from 150 cattle with no known exposure to foot-and-mouth disease (fmd) virus were tested by both the plaque-reduction neutralization (prn) technique and the radial immunodiffusion (rid) technique to evaluate the significance and the extent of cross-reactions in these tests. serums from 30 cattle from each of 5 locations were tested against representative viruses of each of the 7 types of fmd virus. high levels of cross-reactions with both the rid and prn techniques were found in serums of ...1975167625
[studies on the influence of the host system on the result of the neutralization of monodispersed and untreated foot- and mouth disease virus (author's transl)].monodispersed foot- and mouth disease virus was obtained by filtration through membranes having a porosity only twice the diameter of the virus. this monodispersed virus was neutralized by immune sera in the same manner as not treated virus. this was shown by the inactivation curve comparing the amount of virus used for the test (calculated as id50) with the neutralization titre (nd50) or comparing the reduction of infectivity (also calculated as id50) with the dilution factor of the antiserum. ...1975169651
influence of serum restriction on foot-and-mouth disease virus infections in cell cultures. i. enhanced viral susceptibility. 1975169654
influence of serum restriction on foot-and-mouth disease virus infections in cell cultures. ii. altered viral replication. 1975169655
[discovery of the foot-and-mouth disease virus]. 1975171130
[structure of the foot-and-mouth disease virus. 2. various physical and chemical properties of the 12s components].the 12s units were purified by heat treatment and acidification of purified, highly-concentrated virus, with separation of viral rna by gel filtration. the following physical parameters were obtained: - partial specific volume 0.737 +/- 0.020 g/cm3; diffusion soefficient d020,w = 3.96 +/- 0.24 f; sedimentation coefficient s020,w = 12.1 +/- 0.5s; molecular weight 283,00 +/- 30,000 dalton; refraction increment (determined by ultracentrifugation) was dn/dc = 0.189 +/- 0.010 cm3/g for white light of ...1975173253
[immunobiological properties of strains of foot-and-mouth disease virus multiplying in cell cultures]. 1975174272
[sensitivity of cells of the transplanted sheep kidney line to foot-and-mouth disease virus]. 1975174273
[effectiveness of a regime for the pasteurization of milk contaminated with the foot-and-mouth disease virus]. 1975175542
[production of highly active hyperimmune sera against the foot-and-mouth disease virus]. 1975175551
[new cell lines and the adaptation of the foot-and-mouth disease virus to them]. 1975175552
[persistence of the foot-and-mouth disease virus in experimentally infected jackdaws]. 1975175553
proteins induced in bhk cells by infection with foot-and-mouth desease virus.although the infection of bhk cells with foot-and-mouth disease virus did not cause a marked inhibition of cellular protein synthesis, the proteins synthesized gradually changed from host-specific to virus-specific. the synthesis of at least thirteen virus-induced proteins was demonstrated by polyacrylamide electrophoretic analysis of the infected cells. only a small proportion of the virus-induced proteins was incorporated into the mature virus particles. the addition of amino acid analogues ca ...1975164515
the effect of acetylethyleneimine upon a strain of inactivated foot-and-mouth disease virus stored at 4 degrees c.the presence of either thiosulphate-neutralized or free aei was shown to degrade inactivated foot-and-mouth disease virus type o (hong kong) antigen during storage at 4 degrees c. deterioration was evident after 20 weeks of storage and little antigen remained at 36 weeks. optimum stability was obtained by removing the residual inactivant immediately after inactivation.1975166627
effect of pasteurization and evaporation on foot-and-mouth disease virus in whole milk from infected cows.the effects of pasteurization and evaporation on foot-and-mouth disease virus in whole milk from infected cows obtained one day postinoculation were studied. virus survived the heating of milk at high temperature-short time pasteurization at 75 degrees c for 15-17 seconds. in addition, virus from infected milk survived heating at 80 degrees c for the same time. infective virus also survived in the pasteurized milk after evaporation at 65 degrees c to 50% of the original volume. the bovine udder ...1975166740
a genetic recombination map of foot-and-mouth disease virus.sixty ts mutants were isolated from the pacheco strain of type o foot-and-mouth disease virus after treatment with either 5-fluorouracil or hydroxylamine. the conditions affecting recombination and assay of the ts+ recombinants were standardized. using two ts mutants resistant to guanidine, three-factor crosses, supported by two-factor crosses, located 34 of the mutations in a linear arrangement. the recombination frequencies between certain pairs of mutations were additive. the guanidine charac ...1975167118
[study of the indirect complement fixation test for use in the differentiation of foot-and-mouth disease viruses and the determination of immunity].studies were carried out to establish the optimal conditions for the indirect complement-fixation test (icft) and explore the possibility to use the test in differentiating the foot-and-mouth disease (fmd) viruses and checking the immunity obtained in survivals or in animals that had been vaccinated against fmd. it was demonstrated that the proper use of icft necessitates in turn the use of a minimal amount of antigen, which in the presence of 1-2 e homologous hyperimmune serum completely binds ...1975179191
binary ethylenimine as an inactivant for foot-and-mouth disease virus and its application for vaccine production.foot-and-mouth disease virus was inactivated with binary ethylenimine formed apart from or directly in the virus suspension by the cyclization of 2-bromoethylamine hydrobromide or 2-chloroethylamine hydrochloride under alkaline conditions. the inactivation rates with binary ethylenimine prepared apart from the virus suspension in dilute sodium hydroxide with either 2-bromoethylamine hydrobromide or 2-chlorethylamine hydrochloride were higher than with pure ethylenimine. when binary ethylenime wa ...1975167679
the survival of foot-and-mouth disease virus in open air conditions.the influence of the open air factor (oaf) and daylight on the survival of foot-and-mouth disease (fmd) virus held as captured aerosols on spider microthreads has been investigated. virus inactivation due to oaf was slight. similarly, the effect of daylight on the survival of virus was not marked. the results are discussed in relation to the airborne spread of fmd virus in nature.1975168250
immune and antibody responses to an isolated capsid protein of foot-and-mouth disease virus.the purified capsid proteins vp1, vp2, and vp3 of foot-and-mouth disease virus type a12 strain 119 emulsified with incomplete freund's adjuvant were studied in swine and guinea pigs. swine inoculated on days 0, 28, and 60 with 100-mug doses of vp3 were protected by day 82 against exposure to infected swine. serums from animals inoculated with vp3 contained viral precipitating and neutralizing antibodies, but such serums recognized fewer viral antigenic determinants than did antiviral serums. cap ...1975171309
[electron microscopy demonstration of fibrillar structures in the foot-and-mouth disease virus].purified fmd virus was heat treated and then examined by electron microscopy with the negative contrast technique. fibrils of varying length and thickness were present; they were not present after ribonuclease treatment. they were similar to the fibrils described for other picornaviruses. viral rna was the essential component of these artificially arranged fibrils, which were composed of threads about 10 a wide, separated from one another by a gap of about 10 a.1975173250
the pathogenicity of bovine strains of foot and mouth disease virus for impala and wildebeest.impala (aepyceros melampus) and wildebeest (connochaetes taurinus) were infected with bovine strains of foot and mouth disease virus by intradermolingual inoculation. no clinical signs developed in the impala but mild atypical lesions developed in the tongues of the wildebeest with generalized spread to one foot in two of the eight animals exposed. all the impala but only some of the wildebeest developed viraemia. no virus could be isolated from any tissues in either species after the 7th day fo ...1975167208
[determination of the subtype membership of the epizootic foot-and-mouth disease virus by means of the agar gel diffusion test].comparative studies were carried out by means of the agar gel-diffusion technique (agdt) to establish the antigenic identity between the epizootic foot-and-mouth disease strain (the nch/73 representative strain) and the standard viruses of type a. the epizootic strain was found to belong to the subtype group a5. discussed is the usefulness of agdt in determining the antigenic relations between the foot-and-mouth disease viruses.1975174281
virulence of temperature-sensitive mutants of foot-and-mouth disease virus.a number of temperature-sensitive mutants isolated from two strains of foot-and-mouth disease virus were examined for their virulence in suckling mice. the majority of the mutants were found to be less virulent than the parent virus strains, ranging from slight to total attenuation, but two mutants retained parental levels of virulence. there was no correlation between mutant cut-off temperatures and virulence, or the revertant content of mutant preparations and virulence. it was not always poss ...1975167681
[electron microscopy studies on proliferation of foot-and-mouth disease virus in cell cultures. i. morphologic changes in cell nucleus].the first morphological indication of fmd infection of a cell culture was in the nucleus. components of nucleoli became segregated and were finally present only as remnants. it was not possible to distinguish different stages of segregation, as in the case of entero-virus infections, because of the rapidity of fmd virus proliferation. following changes in nucleoli there was margination of chromatin. particularly striking was an increase in interchromatin granules. changes in the nuclear membrane ...1975172041
[electron microscopy studies on the proliferation of foot-and-mouth disease virus in cell cultures. ii. changes in nucleic acid metabolism of the cell nucleus].bhk cells were infected with fmd virus and treated with tritium-labelled thymidine and uridine for examination by autoradiography under the electron microscope. labelling of the dna, examined by autoradiography under the optical microscope, showed inhibition of 3h-thymidine incorporation. for demonstrating rna labelling of nuclei, some cells were treated with actinomycin d and others were left untreated. under the lectron microscope there was no evidence of increased 3h-uridine incorporation in ...1975172042
[electron microscopy studies on the proliferation of foot-and-mouth disease virus in cell cultures. iii. morphogenesis in cytoplasm].the previous parts have been concerned with the participation of the cell nucleus in the formation of the rna of fmd virus. however, the actual morphogenesis of the virus takes place in cytoplasm. in bhk cells, changes attributable to virus infection were visible by the second hour, with the formation of threads and large polysome complexes near the nucleus. viral particles soon appeared between these structures. there were no pronounced foci of viroplasma, and it seemed that they were not neces ...1975172043
variation among strains of type a foot-and-mouth disease virus in the eastern mediterranean region 1964-1972.variants of type a fmd virus from the eastern mediterranean region over the years 1964-72 have been shown to belong to a group distinct from the western european strains as represented by a5 westerwald. this group appears to derive from the a22 strain first recognized in 1964 and indicates the possibility of new strains supplanting old in the field.1975172558
[cytological characteristics of a bovine kidney cell culture chronically infected with foot-and-mouth disease virus (author's transl)].a virus-carrier state was observed in the process of subcultivation of foot-and-mouth disease virus-infected calf kidney cell culture. cytologically, the chronically infected culture differed from the control culture by the presence of a large number of syncytial cells which did not die in acute infection because of poor susceptibility to the virus. chronic infection appeared to be maintained by occasionaly polygonal cells which retained the initial sesceptiblity to foot-and-mouth disease virus. ...1975174327
[comparative serological study of the principal strains of the foot and mouth disease virus isolated in ethiopia 1969-1974]. 1975181797
n-terminal amino acid sequences in the major capsid proteins of foot-and-mouth disease virus types a, o, and c.sequences of amino acids at the n-termini of virus proteins vp1, vp2, and vp3 were determined for foot-and-mouth disease virus types a12 strain 119, o1brugge and c3resende. in the polyacrylamide gel electrophoresis system used to purify the proteins, vp3 migrated faster than vp1 or vp2; and in the virion, vp3 could be cleaved by trypsin into vp3a and vp3b. the n-terminal amino acids for each of the virus types were glycine in vp1, aspartic acid in vp2, and threonine in vp3. no divergences in seq ...1975171452
a major difference in the strategy of the calici- and picornaviruses and its significance in classification.pig kidney (1brs-2) cells infected with vesicular exanthema virus (vev), a calicivirus, did not contain any large precursor polypeptides similar to those found when they were infected with foot-and-mouth disease virus (fmdv). the largest induced protein found in the vev-infected cells had a molecular weight identical with that of the virus structural polypeptide. this difference in strategy between vev and fmdv, taken in conjunction with the morphological and structural differences described pre ...1975178628
evidence for recombination between two different immunological types of foot-and-mouth disease virus.recombination was observed between temperature-sensitive (ts) mutants of two immunological types of foot-and-mouth disease virus which were distinguishable by two marker characteristics in addition to their antigenic type. putative ts+ recombinants were isolated and the segregation patterns of their marker characteristics examined. the results are discussed in terms of the origin of new sub-type strains.1975180948
[foot and mouth disease virus]. 1975185664
a comparative chemical and serological study of the full and empty particles of foot-and mouth disease virus.the chemical and serological properties of the full, naturally occurring empty and artificially produced empty particles of foot-and-mouth disease virus, serotype a(subtype 10, strain 16) have been studies. the full 146s particles comprised the virus rna, three polypeptides (vp1 to vp3) mol. wt. about 30 x 10-3, one polypeptide (vp4) mol. wt. about 13-5 x 10-3, and a small amount of a polypeptide (vpo) mol. wt. about 43 x 10-3. the naturally occurring 75s empty particles contained no rna and muc ...1975235596
temperature-sensitive mutants of foot-and-mouth disease virus: the isolation of mutants and observations on their properties and genetic recombination.a number of temperature-sensitive mutants were isolated from two strains of foot-and-mouth disease virus (fmdv). various properties of the mutants were examined including comparative growth curves at permissive and restrictive temperatures, cut-off temperatures, thermal lability and ph sensitivity. recombination was observed between various pairs of mutants of fmdv strain pacheco. it occurred early in the growth cycle and the proportion of recombinants remained constant thereafter. maximum recom ...1975237977
[occurrence of an increased reaction to challenge infection after vaccination with aerosols of inactivated foot-and-mouth disease virus]. 1976961209
studies on the susceptibility to foot--and--mouth disease virus of bhk cell cultures derived from various sources.cultures of bhk monolayer and suspension cells obtained from a number of laboratories and a group of cloned sub-lines derived from suspension cells were examined for their susceptibility to three fmd virus strains. it was found that the various cultures were sensitive to the test virus strains to differing degrees. it was shown possible to obtain a clone with high susceptibility to asia 1 iran 1/73 virus from a parent culture which had a low susceptibility to that virus. the implication of these ...1976198315
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