Publications

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inter-laboratory validation of foot-and-mouth disease diagnostic capability in germany.germany has been free from foot-and-mouth disease virus (fmdv) without vaccination since 1992, but diagnostic capability at regional laboratories is maintained for fmdv exclusion in suspect cases and as surge capacity for outbreak preparedness. a proficiency test was initiated in 2015 to evaluate the diagnostic performance of 20 regional veterinary laboratories. a panel of two identical samples of fmdv genome for real-time reverse transcription polymerase chain reaction (rt-pcr), four lyophilize ...201728619169
adaption of fmdv asia-1 to suspension culture: cell resistance is overcome by virus capsid alterations.foot-and-mouth disease virus (fmdv) causes a highly contagious disease with catastrophic economic impact for affected countries. bhk21 suspension cells are preferred for the industrial production of fmdv vaccine antigen, but not all virus strains can be successfully propagated in these cells. serotype asia-1 is often affected by this phenomenon. in this study, the asia-1 strain shamir was used to examine viral, cellular and environmental factors that contribute to resistance to cell culture infe ...201728820470
galectin-8 activates dendritic cells and stimulates antigen-specific immune response elicitation.galectin-8 (gal-8) is a mammalian β-galactoside-binding lectin, endowed with proinflammatory properties. given its capacity to enhance antigen-specific immune responses in vivo, we investigated whether gal-8 was also able to promote apc activation to sustain t cell activation after priming. both endogenous [dendritic cells (dcs)] and bone marrow-derived dcs (bmdcs) treated with exogenous gal-8 exhibited a mature phenotype characterized by increased mhc class ii (mhcii), cd80, and cd86 surface ex ...201728811319
preclinical diagnosis: predicting its ability to improve control of farm-to-farm foot-and-mouth disease transmission in cattle.foot-and-mouth disease (fmd) can cause large disruptive epidemics in livestock. current eradication measures rely on the rapid clinical detection and removal of infected herds. here, we have evaluated the potential for preclinical diagnosis during reactive surveillance to reduce the risk of between farm transmission. we used data from transmission experiments in cattle where both individual animal samples, such as blood, probang, saliva or nasal swabs, and herd level samples, such as air samples ...201728330886
a naturally occurring recombinant enterovirus expresses a torovirus deubiquitinase.enteroviruses (evs) are implicated in a wide range of diseases in humans and animals. in this study, a novel enterovirus (enterovirus species g [evg]) (evg 08/nc_usa/2015) was isolated from a diagnostic sample from a neonatal pig diarrhea case and identified by using metagenomics and complete genome sequencing. the viral genome shares 75.4% nucleotide identity with a prototypic evg strain (pev9 ukg/410/73). remarkably, a 582-nucleotide insertion, flanked by 3c(pro) cleavage sites at the 5' and 3 ...201728490584
single-round infectious particle antiviral screening assays for the japanese encephalitis virus.japanese encephalitis virus (jev) is a mosquito-borne flavivirus with a positive-sense single-stranded rna genome that contains a big open reading frame (orf) flanked by 5'- and 3'- untranslated regions (utrs). nearly 30,000 je cases with 10,000 deaths are still annually reported in east asia. although the jev genotype iii vaccine has been licensed, it elicits a lower protection against other genotypes. moreover, no effective treatment for a je case is developed. this study constructed a pbr322- ...201728394283
isolation of single-domain antibody fragments that preferentially detect intact (146s) particles of foot-and-mouth disease virus for use in vaccine quality control.intact (146s) foot-and-mouth disease virus (fmdvs) can dissociate into specific (12s) viral capsid degradation products. fmd vaccines normally consist of inactivated virions. vaccine quality is dependent on 146s virus particles rather than 12s particles. we earlier isolated two llama single-domain antibody fragments (vhhs) that specifically recognize 146s particles of fmdv strain o1 manisa and shown their potential use in quality control of fmd vaccines during manufacturing. these 146s-specific ...201728861080
development of duplex pcr for differential detection of goatpox and sheeppox viruses.clinically, sheeppox and goatpox have the same symptoms and cannot be distinguished serologically. a cheaper and easy method for differential diagnosis is important in control of this disease in endemic region.201728859636
emergence of an exotic strain of serotype o foot-and-mouth disease virus o/me-sa/ind-2001d in south-east asia in 2015.the o/middle east-south asia (me-sa)/ind-2001 lineage of foot-and-mouth disease virus (fmdv) is endemic in the indian subcontinent and has been reported in the middle east and north africa, but it had not been detected in south-east asia (sea) before 2015. this study reports the recent incursions of this viral lineage into sea, which caused outbreaks in vientiane capital of lao people's democratic republic (pdr) in april 2015, in dak nong, dak lak and ninh thuan provinces of vietnam from may to ...201728856846
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