Publications

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single-round infectious particle antiviral screening assays for the japanese encephalitis virus.japanese encephalitis virus (jev) is a mosquito-borne flavivirus with a positive-sense single-stranded rna genome that contains a big open reading frame (orf) flanked by 5'- and 3'- untranslated regions (utrs). nearly 30,000 je cases with 10,000 deaths are still annually reported in east asia. although the jev genotype iii vaccine has been licensed, it elicits a lower protection against other genotypes. moreover, no effective treatment for a je case is developed. this study constructed a pbr322- ...201728394283
isolation of single-domain antibody fragments that preferentially detect intact (146s) particles of foot-and-mouth disease virus for use in vaccine quality control.intact (146s) foot-and-mouth disease virus (fmdvs) can dissociate into specific (12s) viral capsid degradation products. fmd vaccines normally consist of inactivated virions. vaccine quality is dependent on 146s virus particles rather than 12s particles. we earlier isolated two llama single-domain antibody fragments (vhhs) that specifically recognize 146s particles of fmdv strain o1 manisa and shown their potential use in quality control of fmd vaccines during manufacturing. these 146s-specific ...201728861080
development of duplex pcr for differential detection of goatpox and sheeppox viruses.clinically, sheeppox and goatpox have the same symptoms and cannot be distinguished serologically. a cheaper and easy method for differential diagnosis is important in control of this disease in endemic region.201728859636
emergence of an exotic strain of serotype o foot-and-mouth disease virus o/me-sa/ind-2001d in south-east asia in 2015.the o/middle east-south asia (me-sa)/ind-2001 lineage of foot-and-mouth disease virus (fmdv) is endemic in the indian subcontinent and has been reported in the middle east and north africa, but it had not been detected in south-east asia (sea) before 2015. this study reports the recent incursions of this viral lineage into sea, which caused outbreaks in vientiane capital of lao people's democratic republic (pdr) in april 2015, in dak nong, dak lak and ninh thuan provinces of vietnam from may to ...201728856846
rnai combining sleeping beauty transposon system inhibits ex vivo expression of foot-and-mouth disease virus vp1 in transgenic sheep cells.foot and mouth disease, which is induced by the foot and mouth disease virus (fmdv), takes its toll on the cloven-hoofed domestic animals. the vp1 gene in fmdv genome encodes the viral capsid, a vital element for fmdv replication. sleeping beauty (sb) is an active dna-transposon system responsible for genetic transformation and insertional mutagenesis in vertebrates. in this study, a conserved vp1-shrna which specifically targets the ovine fmdv-vp1 gene was constructed and combined with sb trans ...201728855524
cross-species hybridization of foot-and-mouth disease virus-infected bhk-21 cells using human and mouse oligonucleotide microarrays.foot-and-mouth disease virus (fmdv) has a dual capacity to induce either acute or persistent infection in host animals. establishment of an in vitro cell model of fmdv persistent infection facilitates the study of the mechanism underlying this type of infection. in this study, we analyzed gene expression profiles of both acute and persistent infections using cross-species microarrays. our data suggest that human microarrays are more efficient than mouse microarrays in hybridization with cdna fro ...201728854794
enrichment of viral nucleic acids by solution hybrid selection with genus specific oligonucleotides.despite recent advances, our knowledge of potential and rare human pathogens is far from exhaustive. current molecular diagnostic tools mainly rely on the specific amplification of marker sequences and may overlook infections caused by unknown and rare pathogens. using high-throughput sequencing (hts) can solve this problem; but, due to the extremely low fraction of pathogen genetic material in clinical samples, its application is only cost-effective in special, rather than routine, cases. in th ...201728852181
genome sequences of seven foot-and-mouth disease virus isolates collected from serial samples from one persistently infected carrier cow in vietnam.several foot-and-mouth disease virus (fmdv) carrier cattle were identified in vietnam by the recovery of infectious virus from oropharyngeal fluid. this report contains the first near-complete genome sequences of seven viruses from sequential samples from one carrier animal collected over the course of 1 year. the characterization of within-host viral evolution has implications for fmdv control strategies.201728839030
defining the relative performance of isothermal assays that can be used for rapid and sensitive detection of foot-and-mouth disease virus.this study describes the first multiway comparison of portable isothermal assays for the detection of foot-and-mouth disease virus (fmdv), benchmarked against real-time reverse transcription rt-pcr (rrt-pcr). the selected isothermal chemistries included reverse transcription loop-mediated isothermal amplification (rt-lamp) and reverse transcription recombinase polymerase amplification (rt-rpa). the analytical sensitivity of rt-lamp was comparable to rrt-pcr (10(1) rna copies), while rt-rpa was o ...201728837842
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