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hypoglycorrhachia and coxsackie b3 meningoencephalitis.the case of a 3-week-old infant with coxsackie b3 meningoencephalitis is discussed. hypoglycorrhachia and neutrophils in the cerebrospinal fluid characterized the first ten days of her illness. coxsackie b3 should be added to the list of enteroviruses that can cause hypoglycorrhachia during infections of the central nervous system in children.1978215030
viral pollution of surface waters due to chlorinated primary effluents.the role of chlorinated primary effluents in viral pollution of the ottawa river (ontario) was assessed by examining 282 field samples of wastewaters from two different sewage treatment plants over a 2-year period. the talc-celite technique was used for sample concentration, and bs-c-1 cells were employed for virus detection. viruses were detected in 80% (75/94) of raw sewage, 72% (68/94) of primary effluent, and 56% (53/94) of chlorinated effluent samples. both raw sewage and primary effluent s ...1978215085
comparative sensitivity of various cell culture systems for isolation of viruses from wastewater and fecal samples.in efforts to define the most sensitive cell culture systems for recovery of viruses from wastewaters, 181 samples were inoculated in parallel into tube cultures of various cell types and were plaqued in bottle and petri dish cultures of three types of monkey kidney cells. polioviruses were recovered most frequently in the rd line of human rhabdomyosarcoma cells, group a coxsackieviruses in rd and human fetal diploid kidney (hfdk) cells, group b coxsackieviruses in the bgm line of african green ...1978215087
isolation of avian encephalomyelitis virus in nigeria. 1976215251
ventricular aneurysms complicating coxsackievirus group b, types 1 and 4 murine myocarditis.suckling swiss webster mice were inoculated with 10(4)tcd50 of coxsackieviruses, group b types 1 or 4. virulent necrotizing myocarditis resulted in 185 infected mice. of the latter group, three (14.3%) nurslings on the 17th and 23rd day after inoculations had left ventricular aneurysms postmortem. none of 61 concurrently matched control mice developed aneurysms. ventricular aneurysm is a suggested but previously undocumented complication of murine, and possibly human necrotizing transmural coxsa ...1979215340
induction of enhanced resistance against encephalomyocarditis virus infection of mice by nonviable mycobacterium tuberculosis: mechanisms of protection.nonviable mycobacterium tuberculosis strain jamaica suspended in oil-droplet emulsions was used to enhance resistance of mice against encephalomyocarditis virus (emcv). the mycobacteria-injected mice were significantly resistant to 50,000 50% lethal doses of emcv. similar concentrations of virus in plasma of normal and mycobacteria-injected mice from 1 to 120 min after injection of emcv showed that resistance was not a result of rapid elimination of virus from the circulation. furthermore, survi ...1978215550
the relative effectiveness of commonly used disinfectants in inactivation of coxsackievirus b5.coxsackievirus b5 in the presence of fetal calf serum was exposed to six commonly used disinfectants for times of 10, 20 and 30 s. at the end of exposure times skim milk neutralized the disinfectant activity, with residual virus assayed by the plaque technique. the six disinfectants considered were javex, sodium hydroxide, ethanol, wescodyne, one stroke ves-phene and sonacide. although 95% (v/v) ethanol was significantly more virucidal than dilutions of the other five disinfectants tested causin ...1978215674
potentiation of coxsackievirus b3 infection in adult mice pretreated with a gold salt.in mice treated with sodium aurothiomalate (myocrisin), prior to infection with coxsackievirus b3, 90% of the animals died by the 11th day postinfection (p.i.). a mortality of 10% was noted in mice receiving myocrisin only, and no deaths occurred in animals infected with virus alone. the highest amount of virus was recovered from the pancreas of myocrisin-treated mice on day 3 p.i. this was over 500-fold higher than the virus titer found in the pancreas of mice infected with virus only. generall ...1978215719
a case of coxsackie a9 virus infection with orchitis.a thirteen-year-old boy with bilateral orchitis was diagnosed as being infected by coxsackie a9 virus by isolation of the virus from a throat swab and a fecal specimen. serologic investigations revealed the development of transitory specific neutralizing igm antibodies against the isotate and of persistent antibodies belonging to the other immunoglobulin classes. an etiology of the orchitis by any of the infectious agents usually found with this condition could be excluded.1978215720
effect of denervation on coxsackie a virus myositis in mice: an electronmicroscopic study.myositis induced by coxsackie a4 and a9 viruses was investigated in the gastrocnemius muscles of suckling mice and adult mice with denervation. denervation markedly increased the susceptibility of adult mice to coxsackie a virus infection, and this effect was initiated as early as 1 day after denervation. light microscopy demonstrated inflammation and necrosis in the denervated gastrocnemius muscle of adult mice, whereas muscle from the contralateral leg showed only infrequent, mild, focal myosi ...1978215939
[poliomyelitis occuring in a previously vaccinated young woman with isolation of a coxsackie type a virus. report of a case]. 1978215970
[detection of an agent similar to rotavirus in infantile gastroenteritis].the etiology of nonbacterial enterovirus infections in children was studied in autumn and winter under conditions of focal outbreaks in moscow hospitals. electron microscopy revealed a reovirus-like agent in concentrated suspensions of feces collected in the acute period of the disease from children suffering from diarrhea. examinations of paired sera from the patients by the cf test with the antigen of nebraska calf disease virus established significant rises in antibody titers in 68.3% of case ...1978216151
[isolation of type 71 enterovirus from patients with a poliomyelitis-like disease during an outbreak in bulgaria].virological examinations of 72 specimens obtained during an outbreak of a poliomyelitis-like disease in bulgaria, 1975, yielded 23 strains of enterovirus type 71 (e71) and 5 strains of enteroviruses of other types. the viruses were isolated in green monkey kidney cell cultures, in newborn cotton rats, newborn white mice and in monkeys. most of e71 strains (16 out of 23) were isolated from the autopsy materials (brains and spinal cords) from 9 children dying of poliomyelitis, meningoencephalitis, ...1978216154
[development of a method of concentrating enteroviruses for virologic studies of meat].a method for concentration of enteroviruses in meat extracts was developed using polyvinylpyrrolidone (molecular mass 10,000), polyethylene glycol (molecular mass 15,000), both in 10% concentration, silicagel with a granule size of 200 mesh in a concentration of 5%, aluminum hydroxide in an amount of centrifuged pellet of 5 ml of original suspension per 10 ml meat extract. when the optimal conditions of meat extract preparation (meat mass: hanks' solution ration 1: 10) were observed, followed by ...1978216155
[course of antibodies in mice, rats and pigs following administration of encephalomyocarditis viruses]. 1978216192
identification of detergents as components of wastewater sludge that modify the thermal stability of reovirus and enteroviruses.the agent in wastewater sludge previously shown to reduce the heat required to inactivate reovirus (r. l. ward and c. s. ashley, appl. environ. microbiol. 34:681--688, 1977) was "separated" from other sludge components and analyzed by infrared spectroscopy. the infrared spectrum of this material was quite similar to the spectra of commercial anionic detergents, and subsequent analyses of the fractionated sludge samples revealed that anionic detergents in sludge were copurified with the virucidal ...1978216308
heat inactivation of enteric viruses in dewatered wastewater sludge.the effect of moisture content on the rates of heat inactivation of enteric viruses in wastewater sludge was determined. the protective effect of raw sludge on poliovirus previously observed (r. l. ward, c. s. ashley, and r. h. moseley, appl. environ. microbiol. 32:339--346, 1976) was found to be greatly enhanced in sludge dewatered by evaporation. other enteroviruses responded in a similar fashion. this effect did not appear to be due merely to the state of dryness of the sludge samples because ...1978216309
coxsackie myocarditis complicating treatment of hodgkin's lymphoma.a fatal case of coxsackie b4 myocarditis complicating treatment of hodgkin's lymphoma in an adult is reported. the virus was isolated from the myocardium obtained at autopsy and light microscopy confirmed a myocarditis with involvement of the conduction system. electron-microscopy showed dense mitochondrial inclusions. coxsackie myocarditis rarely results in death of affected adults. it is postulated in this case that the treatment of the hodgkin's lymphoma resulted in b lymphocyte depletion, al ...1978216341
virus-induced diabetes in mice: a quantitative evaluation of islet cell population by immunofluorescence technique.the endocrine cell populations of pancreatic islets in encephalomyocarditis (emc)-virus infected mice were assessed quanititatively by immunofluorescence using specific antisera against 4 islet hormones. a marked reduction of the volume of insulin-containing (b-) cells (up to one tenth of control values) was observed at all stages studied in the hyperglycaemic mice. this was accompanied by the inversion of the normal ratio between b- and non b-cells. the volume of the latter cell types was also ...1978216598
countercurrent immunoelectrophoresis in the diagnosis of viral infections of the central nervous system.countercurrent immunoelectrophoresis was utilized in the study of 621 specimens of cerebrospinal fluid to determine the correlation of detection of viral antigens with the clinical diagnosis of aseptic meningitis and related viral infections. a panel of viral antisera was immunoelectrophoresed against 119 specimens from patients with suspected viral infections of the central nervous system (group i), 32 from patients with bacterial meningitis (group 2), and 470 from patients with no suspected in ...1978216755
[epidemic of hand, foot and mouth disease in gifu prefecture in 1973 (author's transl)]. 1978217172
[pancreatic lesion in newborn mice caused by coxsackie b1 virus].changes in the pancreatic gland manifested by necrosis of excretory cells and marked atrophy of insulae with the loss of beta-cells producing insulin were observed experimentally in suckling mice infected with coxsackie b1 virus. it is suggested that the above changes of the pancreas may be a model of human diseases caused by coxsackie viruses, including a model of development of some cases of diabetes.1978217317
assessment of coxsackievirus b3 ts mutants for induction of myocarditis in a murine model.ten temperature-sensitive (ts) mutants isolated from a myocarditis-inducing wild-type (wt) coxsackievirus b3 parent did not induce myocarditis in adolescent cd-1 mice. an avirulent prototype ts mutant from one of the three complementation groups adsorbed to murine cardiac tissue, as did wt virus. heart tissues from mice inoculated with wt virus contained 100- to 1,000-fold more virus than heart tissues from mice inoculated with any of the three prototype ts mutants. wt virus exhibited a greater ...1979217832
enteric viruses in the danube river water and sludge.a study of the viral pollution of the roumanian course of the danube river was carried out from 1972 to 1974. the presence of enteric viruses was investigated in 123 water samples, taken by the gauze swab method and in 116 sludge samples from the bottom of the river. the samples were collected from 20 sampling points along the river from moldova veche to brăila, in accordance with the water use and the main pollution sources. viruses were recovered from 17.5% of the samples. the frequency of vir ...1978217928
comparison of the efficiency of two methods for virus concentration from river water environment in a model experiment.two of the methodical approaches for detection of virus contamination of surface waters were experimentally tested, i.e., the method of adsorption of virus particles to an inorganic salt (cahpo4) and the ultrafiltration method using the amicon apparatus. under the given experimental conditions, the two methods showed practically identical factors of efficiency of virus concentration; the danube river water proved to be a more suitable medium for the model of coxsackie a4 virus than the physiolo ...1978217929
coxsackie a 13 virus in the foci of rheumatism.faeces and nasopharyngeal washing were examined in 12 patients and 27 contacts of these patients in family foci (primary foci) of rheumatism and in 37 patients and 32 contacts of these patients in secondary foci of rheumatism (in hospitals). results of stimultaneous examinations of 127 children of the control groups served as the control. an analysis of the results of the investigation showed the coxsackie a 13 virus was found considerably more frequently in both the patients (83.3%) and their c ...1978217930
easily hydrolyzable, water-soluble derivatives of (+/-)-alpha-5-[1-(indol-3-yl)ethyl]-2-methylamino-delta2-thiazoline-4-one, a novel antiviral compound.the preparation of a series of indole n-acyl and n-carbamic esters of (+/-)-alpha-5-[1-(indol-3-yl)ethyl]-2-methylamino-delta2-thiazolin-4-one (1) is reported. these derivatives were synthesized as potential water-soluble precursors of the antiviral thiazolinone 1, for evaluation by intranasal administration against influenza and other respiratory infections caused by viruses. salts of the basic carbamic esters (16--19) possess the required water solubility, undergo rapid hydrolysis and decarbox ...1979218010
[network for the epidemiological survey of the enteroviruses in sewage (author's transl)].poliomyelitis still occurs each year in france: 27 cases in 1973, 21 cases in each of 1974 and 1975, 8 cases in 1976. because of the risk, a survey of the spread of poliovirus in healthy populations and in sewage was organized by the ministry of health according to the departmental note dgs/hp/1st/250, 9-2-73. the laboratoire d'epidémiologie virale of the laboratoire national de la santé was in charge of the technical aspects of the survey. a preliminary questionnaire was sent to each d.d.a.s.s. ...1978218261
[biological characteristics of the plaque variants of the swine vesicular disease virus].the swine vesicular disease virus yields a heterogenic plaque population consisting of large round plaques of 8-10 mm, small uniform plaques with slightly indented contours, measuring 1-3 mm, and single plaques of transient form and size. the reisolates of the large (lpf) plaques give a population that is similar to the initial virus, while the cloning of the small (mpf) plaques leads to a homogenic population of such plaques. in vivo, the virus of the large plaques manifests enhanced virulence ...1978218333
detection of enteric viruses in solid waste landfill leachates. 1979218468
viral pericarditis in patients receiving hemodialysis.four patients with end-stage renal disease in whom pericarditis developed while they received intermittent hemodialysis therapy were evaluated for viral infection. we found high or rising serum antibody titers to influenza virus a (three patients) and coxsackievirus b (one patient). cardiac tamponade occurred in three patients, requiring pericardiectomy in two; each patient eventually recovered. viral pericarditis may be an important cause of "uremic" pericarditis in chronically dialyzed patient ...1979218513
experimental swine vesicular disease, pathology and immunofluorescence studies.two day old piglets were inoculated intravenously with 1 ml of swine vesicular disease virus uk-g 27-72 isolate. using infectivity tests, immunofluorescent staining and gross and histopathological examination, pathogenesis of the infection was studied in tissue specimens collected daily from one through seven days postinoculation. swine vesicular disease virus had a strong affinity for the epithelia of the tongue, snout, coronary band and lips, the myocardium and the lymphoid elements of the ton ...1979218706
bovine enterovirus cpe at different multiplicities of infection in the absence of viral rna synthesis.when bovine enterovirus (bev) rna synthesis was inhibited by 2-(alpha-hydroxybenzyl)-benzimidazole (hbb), an increase in moi was accompanied by an enhancement of cytopathology. since bev rna synthesis is inhibited and bev protein synthesis is allowed to proceed in the presence of hbb, these results support the concept that proteins play a role in the development of cytopathology independently of viral double-stranded rna.1979218903
the effect of relative humidity on swine vesicular disease virus in dried films before and during formaldehyde fumigation.swine vesicular disease virus (svdv) did not survive drying at high relative humidities (r.h.) but there was little virus loss at low r.h. purified virus dried in films was inactivated by formaldehyde fumigation only at high r.h. inactivation was also influenced by the suspending medium from which the virus was dried. purified virus resuspended in distilled water and then dried, was rapidly killed, but that in tissue culture fluid survived.1979219108
the inactivation of viruses in cattle and pig slurry by aeration or treatment with calcium hydroxide.porcine enterovirus type 2 or porcine adenovirus type 3 were seeded into samples of pig slurry, and a bovine enterovirus was seeded into cattle slurry, and samples of the slurry were aerated in the laboratory for 21 days. the viruses were inactivated more rapidly in the aerated slurry than in control slurry which was not aerated. the difference in inactivation rate was greatest for the porcine adenovirus and least for the bovine enterovirus. inactivation of the porcine enterovirus in aerated dis ...1979219109
[hand-foot and mouth disease (author's transl)]. 1978219112
etiology of pediatric gastroenteritis in rochester, minnesota.the cause of diarrhea in a rochester, minnesota, pediatric population was studied by examining specimens for the presence of enterotoxigenic strains of escherichia coli and reovirus-like agents, in addition to parasites and other bacteria and viruses. reovirus-like agents were detected by electron microscopy in the feces of 11 of 31 patients, but none was found from specimens collected during convalescence or from 16 asymptomatic matched controls (p less than 0.01). infection due to reovirus-lik ...1979219302
[viruses in water (author's transl)]. 1978219306
isolation of a virus from the pancreas of a child with diabetic ketoacidosis.a healthy 10-year-old boy was admitted to the hospital in diabetic ketoacidosis within three days of onset of symptoms of a flu-like illness. he died seven days later and post-mortem examination showed lymphocytic infiltration of the islets of langerhans and necrosis of beta cells. inoculation of mouse, monkey and human cell cultures with homogenates from the patient's pancreas led to isolation of a virus. serologic studies revealed a rise in the titer of neutralizing antibody to this virus from ...1979219345
the etiology of diabetes mellitus. 1979219346
limited persistence of viral antigen in coxsackievirus b3 induced heart disease in mice. 1979219431
enterovirus survey before and after poliomyelitis vaccination in kuala lumpur, malaysia.stool samples from healthy children mainly of the low income group aged 0 to 7 years of age from five maternal and child health centres in kuala lumpur were obtained for isolation of enteroviruses. the specimens were collected before and after the mass vaccination given in the face of polio type 1 epidemic which started in october, 1971. the prevelance rate of enteroviruses was 11.9% (3.0% polioviruses, 8.9% non-polio enteroviruses) before the vaccination and essentially the same after. coxsacki ...1978219550
encephalomyocarditis virus-induced diabetes mellitus treated by islet transplantation.there is considerable evidence that at least some cases of juvenile onset diabetes mellitus in humans are a result of viral infection. viral-induced diabetes in mice may provide an experimental counterpart more similar to the clinical situation than chemical-induced diabetes. our experiments in such mice indicate that islet transplantation is effective in ameliorating viral-induced diabetes and is encouraging for ultimate clinical application of islet transplantation to juvenile onset insulin-de ...1979219567
[delayed hypersensitivity in experimental enterovirus infection]. 1978219619
[method of determination of the number of enteroviruses present in natural waters].the effectiveness of concentratin of enteroviruses present in water by adsorption on membrane filters, ion-exchange resin ab-17-8 and bentonite was compared with set concentrations of virus in water (0.05-0.1-0.5-2.5 and 12.5 pfu/ml). the effectiveness of concentration was shown to depend upon the initial dose of virus present in water. with virus adsorption on membrane filters and ionexchange resin the effectiveness of concentration was the higher (100 and 74%) the lower the virus dose in water ...1978219620
[study of possible viral etiology of crohn's disease]. 1978219622
[experimental study of coxsackie-streptococcal infection].the pathological process due to coxsackie a18 virus and hemolytic streptococcus infection was studied in adult white mice. their synergistic effect was established as manifested by longer periods of virus detection in the blood and heart and the detection of the streptococcus in animals infected with an 8-fold lower dose. in cases of combined infection, signs of dystrophy, destruction and proliferation developed in the myocardium reaching the highest intensity when the virus and the microbe had ...1978219625
[detection and persistence of human-pathogenic viruses in chemical sludge (author's transl)].virological investigations, using poliovirus type i, were carried out on the detection of enterovirus and their persistence in the chemical sludge from a tertiary treatment process, which could be suitable for agricultural and horticultural applications.--a comparison of six eluents showed that tryptose phosphate broth yielded best results allowing approximately 30% overall recovery with the method described. with sludge of low virus content a concentration was possible with alginate filters and ...1978219641
neutralization of aggregated strains of enterovirus 71 and echovirus type 4 in rd and vero or gmk-ah1 cells.strains of enterovirus 71 and echovirus type 4 containing aggregates which were poorly neutralized by antibody in cell lines of green monkey kidney origin such as vero and gmk-ah1, were as easily neutralized as non-aggregated strains when grown in human rd cells, the explanation for this may be a lesser degree of aggregation of viral materials originating from rd cells and/or the use of lower doses of virus for neutralization tests in such cells. the latter was a consequence of a higher sensitiv ...1979219662
[possible role of viral infection in the pathogenesis of urinary tract diseases in pregnant women]. 1979219724
immune response of mice to coxsackie b4 virus infection. 1978220187
viral induced cytopathic effect & release of lysosomal enzyme. 1978220188
travelers' diarrhea among u.s. army troops in south korea.a prospective study of diarrhea was conducted among 98 u.s. army soldiers during their first six weeks in south korea. diarrhea developed in 54 (55%) of 98 soldiers and had a mean duration of five days. infections with salmonella, shigella, vibrio, enterotoxigenic escherichia coli, enteroviruses, and intestinal parasites were uncommon. four (8%) of 50 soldiers with documented diarrhea, two (6%) or 32 with a history of diarrhea, and one (3%) of 29 who denied gastrointestinal symptoms had serologi ...1979220334
enterovirus infection in a semi-closed community.infection with enteroviruses was studied over a 61-week period (during 1960-61) in a semi-closed child community in the detention home of the allegheny county juvenile court in pittsburgh, pennsylvania. while most of the viruses isolated were known adeno- and enterovirus types, three apparently represent new enterovirus types or 'prime strains'. viruses were isolated in all but 6 weeks of the 61-week study period from the gastro-intestinal tracts of 110 children out of a total population of 514 ...1979220514
[intra-hospital infection with viral meningitis, in particular meningoencephalitis, caused by coxsackievirus b1 at the children's unit of the hospital in vrsac]. 1979220517
acute viral myocarditis. clinical and histologic changes. 1979220518
[virological study of the waters from the metropolitan area of san jose, costa rica]. 1978220693
antibody studies in the kuru region. immunological epidemiology of population groups in and adjacent to the kuru region in papua new guinea. i. enteroviruses.a serological survey is reported for antibodies to coxsackie, echo and polioviruses on sera collected in the kuru region of papua new guinea during earliest european contacts with kuru-affected and control populations through the period of highest kuru incidence. the results are discussed in the light of increasing european contact over the period of investigation. suggestions are made concerning possible immunization programs with polioviruses.1978220814
rat interferons. vi. heterologous interferon in the treatment of viral infections.in previous studies in vitro we demonstrated the protection of mouse cells against viral infections by rat interferon. in continuation, the present paper reports results of a study designed to confirm this heterospecific activity of rat interferon in vivo. experiments were carried out with mice of the inbred 129/aoboy strain, inoculated intranasally or intraperitoneally with emc-strain col mm, vsv and influenza a 055/74 viruses, and treated with mouse or rat interferon administered by the same r ...1979220932
pathogenesis of chronic myocarditis in mice infected with coxsackie b3 viruses.infection of 300 mice of the swiss race with coxsackie b3 viruses gave rise to chronic myocarditis and endocarditis. the virus was cultured from the heart muscle 9 days post infection. between days 18 and 108 post infection, virtually all mice showed evidence of an active inflammatory process in the myocardium, and in one half there was proliferation of endothelial cells, and infitration and fibrosis in the endocardium. immunomorphologic studies demonstrated the precence of antiheart antibodies ...1979220933
trials of treating myocarditis in mice infected with coxsackie b3 viruses.on the assumption of an immunologic background of chronic myocarditis after infection with coxsackie b3 viruses, mice were treated with prednisolone or cyclophosphamide, starting 18 days after infection, for periods of three months. the favorable therapeutic effect indicates that chronic myocarditis is an immunologic disease.1979220934
csf viral antibodies. evaluation in amyotrophic lateral sclerosis and late-onset postpoliomyelitis progressive muscular atrophy.serum and csf from 48 patients with amyotrophic lateral sclerosis and six patients with late-onset postpoliomyelitis progressive muscular atrophy were investigated for the presence of antibody to poliovirus types 1, 2, and 3, coxsackie viruses b3 and b4, influenza a, measles, rubella, mumps, herpes simplex types 1 and 2, cytomegalovirus, varicella-zoster, and toxoplasma gondii. these results were compared with those from 53 control patients with neuromuscular disease matched for age, sex, race, ...1979220938
etiology of infantile enteritis in south africa.infantile enteritis constitutes a major health problem in developing countries. several investigations into the etiology of this condition among various south african populations have been undertaken during the past few years. recent studies of black urban infants have revealed that salmonellae, shigellae, enterotoxigenic enterobacteriaceae, and rotaviruses play a relatively minor role in infantile enteritis. on the other hand, all studies, including a number performed several years ago, have de ...1979221438
isolation of picornavirus from feces and semen from an infertile bull.virus was isolated from semen and fecal samples from a bull with orchitis, testicular degeneration, aspermatogenesis, and loss of libido. both isolates were classified as picornavirus, bovine enterovirus serotype i, on the basis of physical, chemical, and serologic characteristics. veterinary practitioners that may suspect viral infection as a cause of bovine infertility should submit both semen and fecal samples for virus isolation and identification.1979221445
fractionation and immunologic assessment of kcl-extracted cardiac antigens in coxsackievirus b3 virus-induced myocarditis.hypertonic salt extracts prepared from the heart tissues of adolescent cd-1 mice were fractionated on sephadex g-100 columns. two separate fractions were obtained. fraction i, containing the antigenic immunoreactive activity, was able to inhibit the migration of cvb3-ppd immune mouse peritoneal exudate cells (impec) as well as pec from mice infected with cvb3 virus alone. fraction ii did not have antigenic activity as assessed by the agarose droplet cell migration inhibition assay. as controls, ...1979221592
migration of epithelial cells in the small intestine of mice perorally infected with coxsackievirus b5.the rate of cell migration in the small intestine during enteric viral infections has not been assessed previously. cd-1 mice (33 days old) were infected perorally with 1.0 x 10(8) plague-forming units of coxsackievirus b5 and 12 hr later were injected intraperitoneally with 2 micron ci of [3h]thymidine/g of body weight. after 2, 12, 24, 48, 60, and 72 hr, mice were killed, and the small intestine was removed. specimens obtained at each interval were examined by radioautography; similar specimen ...1979221595
neonatal 'herpangina'. 1979221634
effect of human and murine neutrophils on encephalomyocarditis, vesicular stomatitis, and reo type 3 virus infections in tissue culture. 1978221657
[hand, foot and mouth disease (author's transl)]. 1979221765
[the antiviral activity of performic acid (author's transl)].under the experimental conditions chosen by the authors, it was demonstrated that the antiviral activity of performic acid against coxsackie virus b 1 is greater than that of peracetic acid.1979221942
[myocarditis caused by viruses]. 1979221962
virus isolated from juvenile diabetic. 1979221976
non-polio enterovirus activity in wisconsin based on a 20-year experience in a diagnostic virology laboratory.laboratory test results, demographic information, and clinical data on 1364 patients who had specimens submitted to the wisconsin state laboratory of hygiene and from whom non-polio enteroviruses were isolated during 1957--1976 were examined. echoviruses (echo) and coxsackievirus group b (cb) and group a (ca) were isolated from 719, 389, and 256 of the patients, respectively. thirty-five different serotypes were identified in the 20-year period. the six most frequently occurring serotypes (echo ...1979222136
ten clinical cases of human infection with venezuelan equine encephalomyelitis virus, subtype i-d.the clinical and laboratory findings in ten humans infected with venezuelan equine encephalitis virus, subtype i-d, are described in this report. clinical and laboratory data indicate that, in contrast to equine infections, human infection with these enzootic virus strains (i-d) is similar to human infection with epizootic strains (i-abc). in most cases there was an abrupt onset of fever, muscle pain, and vomiting. virus was recovered from sera obtained during the first 3 days of illness. lympho ...1979222156
degradation of poliovirus by adsorption on inorganic surfaces.alteration of the specific infectivity of 3h-labeled ribonucleic acid and 14c-protein labeled poliovirus type 1 by adsorption on inorganic surfaces is investigated by application of kinetic theory to data obtained from sequential extractions of adsorbed virus. some surfaces, e.g., sio2, appear to have no significant effect. on the other hand, cuo substantially decreases the specific infectivity of adsorbed preparations. differences in kinetic plots between 3h-labeled ribonucleic acid and 14c-lab ...1979222209
human enteroviruses in oysters and their overlying waters.the presence of enteroviruses in oysters and oyster-harvesting waters of the texas gulf coast was monitored over a period of 10 months. viruses were detected in water and oyster samples obtained from areas both open and closed to shellfish harvesting. viruses were detected periodically in waters that met current bacteriological standards for shellfish harvesting. no significant statistical relationship was demonstrated between virus concentration in oysters and the bacteriological and physiochem ...1979222210
the spread of coxsackie b1 infection.during june and july 1977 5 junior boys in a boarding school for 800 pupils became ill with a mild infection caused by coxsackie b1. the school had been taking part in a vaccine trial, and paired blood samples had been taken from new entrants in october 1976 and october 1977. 18% of the boys susceptible to the infection developed antibodies. the results suggest that dormitories are more important than the day-to-day contacts in the spread of infection. the advisability of nursing children with k ...1979222220
lesions induced by encephalomyocarditis virus and coxsackievirus b in newborn mice.electron microscopic studies of various tissues from virus-infected newborn mice disclosed a consistent form of viral cytonecrotic ultrastructural lesion in divergent types of cells (myocardial, valvular fibroblastic, aortic, exocrine and endocrine pancreatic, and hepatic cells) due to three different picornaviruses (coxsackieviruses b4 and b1 and encephalomyocarditis virus). the lesion is characterized by numerous membrane-vesicle complexes, margination of nuclear chromatin or nuclear pyknosis, ...1979222229
variation in susceptibility of hela cell lines to coxsackievirus a9.in the course of serial passages for several years a line of uncloned hela cells (a line) showed a gradual decrease in plaquing efficiency by coxsackievirus a9 (ca9 virus), while subcultures prepared from the same line kept frozen at an early passage level (a original line) did not show any change. however, it was observed later that the plaque-forming ability of the a original line (a orig. line) also decreased after serial passages as was observed with the a line. comparing the characteristics ...1979222235
electron microscopic studies of viral pancreatitis in coxsackie b4 virus infected mice. 1979222603
[possibility of using the material of petrianov filters for concentrating enteroviruses from water]. 1979222650
counterimmunoelectrophoresis test for immunoglobulin m antibodies to group b coxsackievirus.a counterimmunoelectrophoresis test was developed for immunoglobulin m (igm) antibodies to group b coxsackievirus (cb) types 1 through 5. the igm precipitin line could be identified and differentiated from the igg line by treating sera with 2-mercaptoethanol. antigen purity was demonstrated by single precipitin lines occurring only to the homologous antigen when tested with type-specific hyperimmune rabbit sera. serum pairs from 19 of 22 patients with documented cb type 1,3,4, and 5 infections w ...1979222806
comparison of cynomolgus and rhesus monkey kidney cells for recovery of viruses from clinical specimens.in a study of virus recovery from clinical specimens, cynomolgus monkey kidney cells demonstrated sensitivity equivalent or slightly superior to rhesus kidney cells for siolation of myxo- and paramyxoviruses, adenoviruses, and enteroviruses.1979222808
[viral infections and the heart]. 1979222933
[virusenteritis in childhood (author's transl)].about 20% of infantile enteritis are caused by bacterial infections. the most cases of non bacterial infantile enteritis are supposed to be viral infections. arguments for the existence of enterotropic viruses arised the first time in 1943. since the middle of the fifties, various kinds of enteroviruses are known to be causative agents of infantile gastroenteritis. the same is true occasionally for some types of adenovirus. in 1973 the norwalk-agent was discovered provocing enteritis mainly in a ...1979222939
seroepidemiology of enterovirus 71 among healthy children near tokyo. 1979223018
etiology of sporadic aseptic meningitis in children in the leningrad area.the etiology of sporadic aseptic meningitis among children in the leningrad area in 1974 was studied. 184 patients and 284 healthy children of the same age groups were studied; altogether 540 stool specimens and 99 cerebrospinal fluid specimens were collected for virus isolations and 162 pairs of sera for virus antibody assays. mumps virus infection was the etiology of the disease only in 5% of cases. the main etiological role of enteroviruses was proved. strains most often associated with disea ...1979223233
successful islet transplantation in spontaneous diabetes.optimism for islet transplantation is based on reversal of diabetes artificially induced in animals by pancreatectomy or beta cell toxins. in naturally occurring diabetes, implanted islets might be destroyed by the etiologic agent of the original disease; e.g., virus infection, genetic factors, or autoimmunity. genetically determined diabetes in obese mice, in fact, is resistant to islet transplantation. since these mice are hyperinsulinemic and not similar to human juvenile onset diabetes (jod) ...1979223249
correlation between the presence of enteroviruses in drinking water and some water quality indicators. 1979223280
growth of the ww strain of theiler virus in mouse central nervous system organotypic culture.myelinated organotypic cultures of mouse central nervous system (cns) provide a favorable milieu for growth of the ww strain of theiler virus (tv). ww-tv induced a cytopathic effect characterized by neuronal destruction and swelling of myelin sheaths. tissue culture medium and homogenates of infected cultures produced neurologic disease in mice, and tv was demonstrated in cultures by indirect immunofluorescence and electron microscopy. ultrastructurally, severe alterations in neurons and the pre ...1979223367
coxsackievirus b epidemic at a boys' camp.an epidemic of coxsackievirus b2 infections occured at a boys summer camp. the resulting illness was characterized by malaise, headache, muscle pain, and high fever that persisted for four to six days. the boy in the index case arrived at the camp the first day of the season. the attack rate was 89% among campers and 47% among counselors. the spread of infections appeared to be on a person-to-person basis and in a disorderly fashion. unusual features of the epidemic included the high attack rate ...1979223433
computerized tomography in infantile encephalitis.although computerized tomographic (ct) findings are well recognized in numerous neurologic diseases of children, ct scan abnormalities of encephalitis have only been associated with herpes simplex infection. we describe two infants, one with coxsackievirus b2 meningoencephalitis and one with clinical encephalitis, whose ct scans were similarly abnormal during the acute phase of their illness. in both infants, recovery of both neurologic and developmental deficits was accompanied by resolution of ...1979223434
demonstration of specific antibodies to a coxsackie-like virus in patients of a hepatitis outbreak.lynchburg fecal virus (lfv), originally isolated from the stool of an infectious hepatitis patient, was passaged 15 times in wi-38 cells and partially characterized. its properties are as follows: rna virus; 27 nm in diameter, picornavirus-like morphology; inactivated at 56 c for 60 minutes; resistant to treatment with hydrochloric acid (ph 3.0), chloroform (33%), and diethyl ether (20%). neutralization studies indicated that lfv is antigenically related to coxsackievirus a-24 but not to prototy ...1979223437
viruses in water. 1979223484
[virological research in acute respiratory diseases in 1976--1977 (author's transl)].from october 1976 to june 1977 virological researches have been carried out on 5 subjects with influenza, on 302 children hospitalized in pediatric departments for respiratory illness and 94 children hospitalized in the same departments for other (non respiratory) diseases. the incidence of influenza was moderate and restricted to small epidemic episodes in school and preschool children. the isolated viruses resulted of a type, substantially similar to the prototype strains appeared from 1972 to ...1978223505
[2 cases of meningoneuraxitis caused by enteroviruses]. 1978223506
antibodies to coxsackie b viruses in congestive cardiomyopathy.fifty patients with congestive cardiomyopathy have been studied for evidence of previous coxsackie b virus infection and compared with age- and sex-matched controls who had been admitted to hospital for investigation of other cardiac diseases. high neutralisation titres (greater than or equal to 1024) to coxsackie b viruses were more common among the controls. on subdividing the patients according to their length of symptomatic history before study, high titres were more common only in those wit ...1979223612
the efficacy of polio vaccination in finland.the inactivated polio vaccine has eliminated not only the disease but also polioviruses from finland. the last endemic cases of poliomyelitis were seen in 1961, and two were imported in 1964. to check whether polioviruses were still circulating in finland, an intensified search for polioviruses was carried out in 1972-1974 in sewage, among patients with polio-like diseases and among healthy preschool children. viruses, mostly coxsackie b viruses, were isolated from sewage in 67% of the samples, ...1978223906
poliomyelitis survey in middle-east countries.the poliomyelitis survey was started in 1976 in bahrain and kuwait. for each paralytic case, diagnosis was done by virus isolation, neutralizing antibody titration and specific igm characterization. more than 90% of the infants who were ill were less than 2 years old. in bahrain, 17 paralytic cases in 1976 and 12 in 1977 occurred between january and july (annual incidence was respectively 7.7 and 5.4/100,000). the first epidemic was due to poliovirus type 2, the second one to poliovirus type1. f ...1978223907
biochemical and ultrastructural changes in a and b cells of the islets of langerhans of mice infected with emc virus. 1979223928
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