retroviral pseudotypes produced by rescue of a moloney murine leukemia virus vector by c-type, but not d-type, retroviruses.human hos cells containing a moloney murine leukemia virus (mo-mlv) recombinant genome were infected by a panel of retroviruses. the c-type viruses simian sarcoma associated virus, feline leukemia virus subgroup b, and the feline endogenous virus rd114 were able to form pseudotypes with the mo-mlv genome, which transferred a selectable marker gene to target cells; however, human t cell leukemia virus-1 and the d-type viruses mason-pfizer monkey virus and simian retrovirus-1 failed to rescue the ...19921733113
type c retrovirus inactivation by human complement is determined by both the viral genome and the producer cell.the inactivation of type c retroviruses by human serum may be a considerable impediment to the use of retroviral vectors in vivo for gene therapy. here we show that virus inactivation is dependent both on the virus and on the cell line used to produce the virus. all viruses produced from murine nih 3t3 or dog cf2ths+l- cells are sensitive to human serum. in contrast, those produced from mink mv-1-lu and human hos or te671 cells are at least partially resistant, with the exception of murine leuke ...19947966590
comparison of efficiency of infection of human gene therapy target cells via four different retroviral receptors.the relative efficiency of transduction of gene therapy target cells was measured for retroviruses bearing the envelopes of amphotropic murine leukemia virus (mlv-a), xenotropic murine leukemia virus (mlv-x), gibbon ape leukemia virus (galv), feline leukemia virus subgroup b (felv-b), and the feline endogenous virus rd114. these viruses use various cell-surface receptors. activated peripheral blood lymphocytes (pbl) and primary melanoma cultures were infected relatively poorly by mlv-x pseudotyp ...19968727505
development of improved adenosine deaminase retroviral vectors.a series of adenosine deaminase (ada) retroviral vectors were designed and constructed with the goal of improved performance over the pa317/lasn vector currently used in clinical trials. first, the bacterial selectable-marker neomycin phosphotransferase (neo) gene was removed to create a "simplified" vector. second, the moloney murine leukemia virus long terminal repeat (ltr) promoter used for ada expression was replaced with either the myeloproliferative sarcoma virus (mpsv) or sl3-3 ltr. super ...19989499026
a sodium-dependent neutral-amino-acid transporter mediates infections of feline and baboon endogenous retroviruses and simian type d retroviruses.the type d simian retroviruses cause immunosuppression in macaques and have been reported as a presumptive opportunistic infection in a patient with aids. previous evidence based on viral interference has strongly suggested that the type d simian viruses share a common but unknown cell surface receptor with three type c viruses: feline endogenous virus (rd114), baboon endogenous virus, and avian reticuloendotheliosis virus. furthermore, the receptor gene for these viruses has been mapped to huma ...199910196349
efficient gene transfer into primary human cd8+ t lymphocytes by mulv-10a1 retrovirus pseudotype.efficient and stable gene transfer into primary human t lymphocytes would greatly improve their use for adoptive transfer to treat acquired disorders, viral diseases, and cancer. we have constructed retroviral vector pseudotypes of amphotropic murine leukemia viruses (a-mulv, mulv-10a1), gibbon ape leukemia virus (galv), and feline endogenous virus (rd114) containing the enhanced green fluorescent protein (gfp) as a marker gene. transduction of primary human cd8+ t lymphocytes by the different g ...200010811229
highly efficient gene transfer into cord blood nonobese diabetic/severe combined immunodeficiency repopulating cells by oncoretroviral vector particles pseudotyped with the feline endogenous retrovirus (rd114) envelope expression of the amphotropic envelope receptor is a recognized barrier to efficient oncoretroviral vector-mediated gene transfer. human hematopoietic cell lines and cord blood-derived cd34(+) and cd34(+), cd38(-) cell populations and the progenitors contained therein were transduced far more efficiently with oncoretroviral particles pseudotyped with the envelope protein of feline endogenous virus (rd114) than with conventional amphotropic vector particles. similarly, human repopulating ...200010942359
rd114-pseudotyped oncoretroviral vectors. biological and physical functional expression of the viral envelope receptor is a recognized barrier to efficient oncoretroviral mediated gene transfer. to circumvent this barrier we evaluated a number of envelope proteins with respect to gene transfer efficiency into primitive human hematopoietic stem cell populations. we observed that oncoretroviral vectors pseudotyped with the envelope protein of feline endogenous virus (rd114) could efficiently transduce human repopulating cells capable of establishing mult ...200111458516
prolonged multilineage clonal hematopoiesis in a rhesus recipient of cd34 positive cells marked with a rd114 pseudotyped oncoretroviral vector.the ability to efficiently transfer a gene into repopulating hematopoietic stem cells would create many therapeutic opportunities. we have evaluated the ability of particles bearing an alternative envelope protein, that of the feline endogenous virus (rd114), to transduce stem cells in a nonhuman primate autologous transplantation model using rhesus macaques. we have previously shown this pseudotyped vector to be superior to the amphotropic vector at transducing cells in umbilical cord blood cap ...200312667996
transduction of bone-marrow-derived mesenchymal stem cells by using lentivirus vectors pseudotyped with modified rd114 envelope glycoproteins.bone-marrow-derived mesenchymal stem cells (mscs) have attracted considerable attention as tools for the systemic delivery of therapeutic proteins in vivo, and the ability to efficiently transfer genes of interest into such cells would create a number of therapeutic opportunities. we have designed and tested a series of human immunodeficiency virus type 1 (hiv-1)-based vectors and vectors based on the oncogenic murine stem cell virus to deliver and express transgenes in human mscs. these vectors ...200414722277
gene transfer to repopulating human cd34+ cells using amphotropic-, galv-, or rd114-pseudotyped hiv-1-based vectors from stable producer cells.a novel, stable human immunodeficiency virus type 1 vector packaging system, star, was tested for its ability to transduce human cord blood cd34+ progenitor cells assayed both in vitro and after transplantation into nod/scid mice. vectors pseudotyped with three different gammaretrovirus envelopes were used: the amphotropic mlv envelope (mlv-a), a modified gibbon ape leukemia virus envelope (galv+), and a modified feline endogenous virus rd114 envelope (rdpro). gene transfer to freshly thawed cd3 ...200515727942
efficient characterisation of human cell-bioceramic interactions in vitro and in vivo by using enhanced gfp-labelled mesenchymal stem cells.human mesenchymal stem cells (hmscs) were transfected using four retroviral pseudotypes, amphotropic murine leukemia viruses 4070 (mulv-10a1), a modification of amphotropic pseudotype 4073 (a71g, q74k, v139m), gibbon ape leukemia virus (galv), or feline endogenous virus (rd114) encoding the neomycin resistance (neo(r)) gene and enhanced green fluorescent protein (egfp) as genetic markers. it was observed that the mulv4073 was the most efficient pseudotype for hmsc transfection. the proliferation ...200515882901
human mesenchymal stem cells (hmscs) expressing truncated soluble vascular endothelial growth factor receptor (tsflk-1) following lentiviral-mediated gene transfer inhibit growth of burkitt's lymphoma in a murine model.efficient gene transfer to bone marrow derived mesenchymal stem cells (msc) would provide an important opportunity to express potent anticancer agents in the tumour microenvironment because of their contribution to the tumour stroma.200616288493
specific and stable gene transfer to human embryonic stem cells using pseudotyped lentiviral vectors.genetic modification of human embryonic stem cells (hescs) is an important tool for understanding and influencing their biologic properties. at the present time, lentiviral vectors pseudotyped with the vesicular stomatitis virus g protein (vsv-g) have been most effective for stable gene transfer to hescs. however, they also efficiently transduce murine embryonic fibroblasts (mef), used to support the undifferentiated state of many commonly used hesc lines. transduction of both the mef as well as ...200616522168
n-linked glycosylation and sequence changes in a critical negative control region of the asct1 and asct2 neutral amino acid transporters determine their retroviral receptor functions.a widely dispersed interference group of retroviruses that includes the feline endogenous virus (rd114), baboon endogenous virus (baev), human endogenous virus type w (herv-w), and type d primate retroviruses uses the human na(+)-dependent neutral amino acid transporter type 2 (hasct2; gene name, slc1a5) as a common cell surface receptor. although hamster cells are fully resistant to these viruses and murine cells are susceptible only to baev and herv-w pseudotype viruses, these rodent cells bot ...200312584318
comparison of various envelope proteins for their ability to pseudotype lentiviral vectors and transduce primitive hematopoietic cells from human blood.substantial effort has been invested in developing methodologies for efficient gene transfer into human, repopulating, hematopoietic stem cells. oncoretroviral vectors are limited by the lack of nuclear mitosis in quiescent stem cells during ex vivo transduction, whereas the preintegration complex of lentiviral vectors contains nuclear-localizing signals that permit genome integration without mitosis. we have developed a flexible and versatile system for generating lentiviral vector particles an ...200211863413
localization of the receptor gene for type d simian retroviruses on human chromosome 19.simian retrovirus (srv) serotypes 1 to 5 are exogenous type d viruses causing immune suppression in macaque monkeys. these viruses exhibit receptor interference with each other, with two endogenous type d viruses of the langur (po-1-lu) and squirrel monkey, and with two type c retroviruses, feline endogenous virus (rd114/ccc) and baboon endogenous virus (baev), indicating that each utilizes the same cell surface receptor (m. a. sommerfelt and r. a. weiss, virology 176:58-69, 1990). vesicular sto ...19902173788
isolation and characterization of retrovirus-like elements from normal human fetuses.retrovirus-like particles have been isolated from normal fetal human plasma and from different embryonic organs collected from late first-trimester fetuses. the majority of the virus-like particles banded at a density region of of 1.19-1.22 g/ml, although lighter particles having a density of 1.15-1.17 g/ml were observed in some fetal tissues. the particles appeared similar to retroviruses when viewed electron-microscopically. they contained reverse transcriptase (rt) which accepted oligo (dg)-p ...19827129678
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