[studies on the effects of rhtnf-alpha anti-replication against ehfv and hav in vitro].it was demonstrated in this study that rhtnf-alpha can inhibit the replication of epidemic hemorrhagic fever virus and hepatitis a virus in tissue culture. ehfv infection produced strong fluorescence ( - +) on vero-e6 cells after 9 days. in the rhtnf-alpha treated group, however the fluorescence was very weak (- or +/-). rhtnf-alpha also showed anti-hav effects on 2bs cells. the fluorescence intensity on 2bs cells infected by hav was - +, but in the rhtnf-alpha treated group, the fluorescence wa ...19921319852
haemorrhagic fever with renal syndrome: clinical aspects.based on clinical and pathological features a typical case of haemorrhagic fever with renal syndrome passes through five phases: (1) febrile phase, (2) hypotensive phase, (3) oliguric phase, (4) diuretic phase and (5) convalescent phase. the major manifestations are fever, pain in the back and abdomen, flushed face, prostration, proteinuria, purpura and haemorrhage and acute renal failure. selective right auricular haemorrhage, marked congestion and haemorrhage in the renal medulla and necrosis ...19921349230
haemorrhagic fever with renal syndrome, virological and epidemiological aspects.haemorrhagic fever with renal syndrome (hfrs) is characterized by fever, headache, abdominal pain, renal dysfunction and various haemorrhagic manifestations. the viruses causing hfrs all belong to the hantavirus genus in the bunyaviridae family. at least three of the different hantaviruses are associated with human disease: hantaan, seoul, and puumala viruses. hfrs is endemic in a belt from norway in the west, through sweden, finland, the soviet union, china, korea to japan in the east. the clin ...19921349231
[influence of moxibustion on txa2 and pgi2 in plasma of rat infected epidemic hemorrhagic fever virus (ehfv)].in this paper, the model of rat infected ehfv was made and the influence of moxibustion on txa2 and pgi2 in its plasma was observed. the results show that the content of txa2 increased and pgi2 decreased in rat significantly after the abdominal inoculation of ehfv, and the content of txa2 decreased and pgi2 increased markedly in rat infected ehfv to normal level after treatment with moxibustion, suggesting that the regulative function of moxibustion on txa2 and pgi2 is one of its nerve-endocrine ...19921356657
[a study on transmission of epidemic hemorrhagic fever virus through skin wounds among rodents in natural conditions].224 rodents captured from endemic areas of epidemic hemorrhagic fever (ehf) in hubei province were examined for the presence of skin wounds and ehf viral antigens and antibodies by immunofluorescent assay (ifa) technique. it was found that 30.8% (69/224) of the captured rodents had skin wounds and that 24.6% (17/69) and 29.0% (20/69) of the wounded rodents were positive respectively to ehf viral antigens and antibodies, as compared with the corresponding rates of 9.0% (14/155) and 8.4% (13/155) ...19921356718
[leptotrombidium scutellare in transmission of epidemic hemorrhagic fever virus].to further elucidate the role of leptotrombidium scutellare in transmission of epidemic hemorrhagic fever virus (ehfv), a series of studies were carried out from 1988 to 1990. ehfv was isolated from both larvae of free mites collected from the grassland of endemic areas and larvae of filial mites hatched in the laboratory. the suckling mice bitten by these larvae were infected by ehfv. because only the larvae of chigger mite can bite vertebrate hosts and only take a full meal in its entire life ...19921363219
[effect of moxibustion on the immune and anti-virus functions of the rat infected with epidemic hemorrhagic fever virus]. 19921364108
[effect of moxibustion on humoral factors in the rat infected with epidemic hemorrhagic fever virus]. 19921364109
bank vole monoclonal antibodies against puumala virus envelope glycoproteins: identification of epitopes involved in vole (clethrionomys glareolus) monoclonal antibodies (mabs) against the two envelope glycoproteins (g 1 and g 2) of the puumala (puu) virus were generated and characterized. analyses of the mabs' antigen and epitope specificities showed non-overlapping reactivities of one anti-g 1 and two anti-g 2 mabs. a significant neutralizing activity was shown by the anti-g 1 and one of the anti-g 2 mabs, suggesting the existence of at least one neutralizing domain on each of the two glycoproteins. the ...19921381914
[study of the cultured human endothelial cells infected by epidemic hemorrhagic fever virus].virus antigen could be detected in the cytoplasm of infected human endothelial cells (hec) by immunofluorescent assay (ifa) 2 to 10 days after the inoculation of epidemic hemorrhagic fever virus (ehfv), but no apparent histologic changes could be found by phase contrast light microscopy, as well as no mature virus particles could be detected under the transmission electron microscope. reinoculation of the freeze-melt supernatant of hec 8 days after the inoculation of ehfv to ehfv susceptible ver ...19921477943
human-rodent contact and infection with lymphocytic choriomeningitis and seoul viruses in an inner-city population.we collected 1180 sera and 1363 questionnaires with information on demography, rodent exposure, and history of travel from persons visiting a sexually transmitted disease (std) clinic in baltimore between 1986-1988. serological tests for two rodent-borne viruses detected antibodies to lymphocytic choriomeningitis virus (lcmv) in 54 individuals (4.7%; n = 1149) and antibodies to seoul virus (seov) in three persons (0.25%; n = 1180). antibody prevalence to lcmv increased with age, but there were n ...19911672798
[antigenic components of the epidemic hemorrhagic fever virus]. 19872443227
[extraction of effective parts of alternanthera philoxeroides (mart.) griseb. and its antiviral effect].several parts of alternanthera philoxeroides were extracted by lead-salt sedimentation and double phase solvent extractive technique and their antiviral effects were studied. the results showed that extracts of petroleum ether, ether, and ethyl acetate have inhibitory effect on epidemic hemorrhagic fever virus (ehfv). the antiviral effective part is coumarin analogue.19892508673
serologic evidence of hantaviral infections within small mammal communities of baltimore, maryland: spatial and temporal patterns and host range.serologic evidence was used to investigate the spatial and temporal distribution and host range of hantaviruses in small mammal communities in baltimore, md. immunofluorescent antibody (ifa) reactive to a baltimore rat isolate of seoul virus was detected in 44% of 404 norway rats captured at 4 park or residential locations; 21% of 121 meadow voles captured at 4 park locations possessed significant ifa titers to prospect hill virus. evidence from plaque neutralization assay of rodent sera suggest ...19892570533
effects of hantaviral infection on survival, growth and fertility in wild rat (rattus norvegicus) populations of baltimore, maryland.survival, growth rates, body size and fertility of wild caught norway rats (rattus norvegicus), infected and uninfected with a hantavirus (antigenically related to seoul virus), were compared. no differences were found in the survival of seronegative versus seropositive rats, as measured by mark-recapture experiments. growth rates, as measured by weight gain but not by increased body length, were slower in seropositive, sexually mature (greater than 200 g) rats, although no differences in the ul ...19892572705
efficacy of inactivated vaccine containing cyto-hemagglutinin against epidemic hemorrhagic fever in rabbits.using the z-10 strain of epidemic hemorrhagic fever virus (ehfv) as seed, and the primary cell of meriones unguiculatus kidney tissue as incubation cell, a propiolactone inactivated epidemic hemorrhagic fever (ehf) vaccine was prepared, according to a similar procedure required for the production of biological products such as the japanese b encephalitis vaccine. besides the ehfv antigen detected by elisa or reversed passive hemagglutination test (rpha) as were used for the formalin inactivated ...19892576866
a clinical study of hemorrhagic fever with renal syndrome caused by seoul virus infection.the clinical findings of 29 patients with hemorrhagic fever with renal syndrome (hfrs) caused by seoul virus were evaluated and compared with the previously reported clinical findings of classic korean hemorrhagic fever (khf). the diagnoses of these patients were made by hemagglutination inhibition test. the results were as follows: 1) the disease occurred predominantly in males with a high incidence in the third and fourth decades of life. 2) the highest incidence of the disease occurred in oct ...19892577257
[the domestic cat as the host animal of the epidemic hemorrhagic fever virus]. 19852870786
hantavirus-related acute interstitial nephritis in western europe. expansion of a world-wide zoonosis.we report the clinical, biological and serological characteristics of 76 cases of hantavirus-induced acute interstitial nephritis diagnosed in belgium and france between 1977 and 1986. the disease is characterized by a fever of sudden onset promptly accompanied by pain in the loin or abdomen, nausea and vomiting, myalgias and occasionally with acute myopia and conjunctival injection. within a week acute renal failure develops, necessitating haemo- or peritoneal dialysis in up to 10 per cent of p ...19892576470
study of hamsters infected with epidemic hemorrhagic fever virus. 19882906853
[transmission of epidemic hemorrhagic fever virus between mice and mites in ehf endemic areas]. 19872898326
[detection of antigens and antibodies of epidemic hemorrhagic fever virus in experimental albino rats in guangzhou city]. 19863091256
propagation of epidemic hemorrhagic fever virus in meriones unguiculatus. 19863100239
[strains of epidemic hemorrhagic fever virus isolated from the lungs of c. russula and a. squamipes]. 19863100244
[identification of epidemic hemorrhagic fever virus in human leukocytes]. 19873127020
cell-mediated immunity to virus causing haemorrhagic fever with renal syndrome: generation of cytotoxic t lymphocytes.cytotoxic t lymphocytes (ctls) were generated when spleen cells from mice infected with viruses causing haemorrhagic fever with renal syndrome were stimulated in vitro with syngeneic cells infected with viruses. these cytotoxic effector cells, with lyt2+ l3t4- markers on their surface, demonstrated h-2 restriction. ctls induced by hantaan virus (76-118 strain) or seoul virus (b-1 strain) showed cross-reactivity with infected target cells. hantaan virus infection induced a higher ctls response th ...19883137309
[study of apodemus (agrarius) as a host of epidemic hemorrhagic fever virus and application of apodemus lung antigen for laboratory diagnosis]. 19826192929
haemorrhagic fever with renal syndrome: clinical, virological and epidemiological perspectives.haemorrhagic fever with renal syndrome (hfrs) is caused by a group of rna viruses within the family of bunyaviridae known as hantaviruses. the classical, severe form of hfrs is characterized by fever, headache, abdominal and lumbar pain, proteinuria, haemorrhagic phenomena, shock and renal failure. the disease is associated with the prototype hantaan virus and occurs in rural areas of korea and china with apodemus mice as reservoir hosts. a clinically less severe form of hfrs, which is caused by ...19872895603
epidemiological investigations following an outbreak of hemorrhagic fever with renal syndrome in greece.epidemiological investigations were conducted following an outbreak of hemorrhagic fever with renal syndrome (hfrs) which occurred in the state of epirus, northwestern greece, in july and august 1983. a total of 8 patients were hospitalized during the outbreak; 3 were severely ill and 1 died. a serosurvey made in may 1984 sampled 184 of the approximately 400 residents of the village of tsepelovo, where 4 patients resided, and found 12 (6.5%) persons, including convalescent sera from 4 patients, ...19862871769
[carriers of epidemic hemorrhagic fever virus in rodents and mites in ehf endemic area]. 19863087624
[human infection by hantavirus in southern and southeastern brazil].the results of serological investigation on hantavírus antibodies carried out between february 1986 and august 1990 in human populations of the states of são paulo and paraná, brazil, which had close contact with urban rats or wild rodents and others small mammals are described. objective--to identify the human infection caused by hantavíruses, which are the etiological agents of hemorrhagic fever with renal syndrome. methods--sera from 1063 persons were tested by enzyme immunoassay (elisa) to h ...19947820156
[a preliminary observation on the antigen of epidemic hemorrhagic fever virus carried by rodents in linyi prefecture]. 19836202417
[experimental animal information file: isolation of epidemic hemorrhagic fever virus]. 19836412637
[morphology of epidemic hemorrhagic fever virus. i. electron microscopic observation of virus in human diploid cell line]. 19836414714
antigenic and molecular characterization of hantavirus isolates from china.hemorrhagic fever with renal syndrome (hfrs) is caused by certain viruses in the genus hantavirus, family bunyaviridae, and is a major public health problem in china. by using molecular and serological tests, we characterized 15 hantaviruses isolated either from patients with hfrs or from rodents captured in endemic areas of china. by cross plaque-reduction neutralization tests performed with rabbit immune sera, we identified two serologically distinct groups of viruses, comprised of those relat ...19947909976
comparison of nucleotide sequences of m genome segments among seoul virus strains isolated from eastern asia.the nucleotide sequences of the m genome segments of three seoul virus strains (ki strains) which were isolated from urban rats inhabiting the same enzootic focus between 1983 and 1988 were compared. the viral cdnas were amplified by pcr and were directly sequenced. the nucleotide sequences of ki strains were extremely homologous regardless of isolation year (less than 10 substitutions in 3651 nucleotides, less than 4 substitutions in 1133 amino acids). in addition, the nucleotide sequence of th ...19947941698
comparison of virulence between seoul virus strain sr-11 and hantaan virus strain 76-118 of hantaviruses in newborn mice.virulence of hantavirus strain of sr-11 seoul virus and hantaan 76-118 (htn) of hantaan virus were compared. infections of both strains were lethal in newborn mice. however, inoculum required to cause lethal infection was about 4,000 times higher for strain htn (1.65 x 10(3) pfu/mouse/ld50) than for strain sr-11 (0.36 pfu). thus, both strains were considered pathogenic to newborn mice but they possessed different levels of virulence. the assay system used for these strains in newborn mice proved ...19937901740
inability of a strain of seoul virus to transmit itself vertically in strain of seoul virus was inoculated into new-born female rats. these rats at the age of 13 weeks were mated with uninfected male rats. the virus was transmitted to the male rats, but the offsprings were not infected from their mather rat owing to the maternal antibody. no intrauterine transmission of the virus was observed. we assumed that sr-11 type strain of seoul virus could not be disseminated among rats in nature.19937914245
rapid differentiation between hantaan and seoul viruses by polymerase chain reaction and restriction enzyme analysis.the majority of causative strains of hemorrhagic fever with renal syndrome (hfrs) are known as hantaan and seoul viruses in korea. the clinical manifestations may be indistinguishable between both viruses, although the clinical course of hantaan virus infection is more severe than that of the seoul virus. therefore, the differentiation of hantaan or seoul virus may be important for predicting the prognosis. the primers were selected from the published sequences of the s segments of hantaan virus ...19947931185
different transmissibility of 2 isolates of seoul virus from the same wild brown rat (rattus norvegicus).two seoul virus strains were isolated from the same wild brown rat (rattus norvegicus) by 2 different procedures. while one isolate (tr-352) by means of a cell culture system had no horizontal transmissibility in 3-week-old rats, another isolate (tr-352r) by means of inoculation to newborn rats had transmissibility in rats.19947948388
domestic cases of hemorrhagic fever with renal syndrome in the united states.although serologic studies have identified hantaviral infection in the united states, acute disease has not been recognized. this study describes 3 cases of domestically acquired hemorrhagic fever with renal syndrome (hfrs) in the united states. infection was due to a local strain of seoul virus (baltimore rat virus). a review of the clinical features indicated a mild illness characterized by nausea, vomiting, renal and liver failure similar to hfrs described elsewhere for rat-borne viruses. fol ...19947991040
[a combined staining of immunohistochemistry and in situ hybridization for detecting epidemic hemorrhagic fever virus in tissue section].a combined staining of immunohistochemistry and in situ hybridization has been successfully applied to detect antigen and viral rna at the same tissue slice from epidemic hemorrhagic fever (ehf) autopsy. multiple mcab were used to detect ehfv antigen, followed with a digoxigenin-labelling cdna probe for detecting ehfv rna respectively. the viral antigen was seen in the cellular cytoplasm and viral rna was demonstrated within the nuclei. it is emphasized that pretreatment of the section with lugo ...19938106176
hemorrhagic fever with renal syndrome caused by the seoul virus.the seoul virus is an important etiologic agent in hemorrhagic fever with renal syndrome (hfrs), and infections with the seoul virus are less severe than those with the hantaan virus. however, the information on hfrs caused by the seoul virus is limited in korea. retrospective clinical analysis was done on 30 patients with seoul virus infection who had been diagnosed as having hfrs by clinical features and serologic testing by the plaque reduction neutralization test from 1986 to 1991 at the seo ...19958587622
seoul hantavirus seropositivity among injecting drug users in baltimore. 19947996013
serological evidence for zoonotic hantaviruses in north carolina a survey of seven species of wild rodents (n = 423) collected between october 1993 and march 1994 from the three principal ecological biomes of north carolina (usa), we found hantavirus antibodies in seven (2%) of 301 peromyscus spp. hantavirus antibodies were detected in p. leucopus and p. maniculatus captured from mountain and coastal island biomes. three mice were positive for sin nombre virus, while four others had antibodies to seoul virus or a related agent. two mice serologically posit ...19968722278
[alternanthera philoxeroides (mavt.) griseb protection against fetal epidemic hemorrhagic fever virus infection in suckling mice].it has been confirmed that alternanthera philoxeroides (apg) can markedly protect suckling mice from being infected by epidemic hemorrhagic fever (ehf) virus. after the infected mice were treated with the effective components their survival rate increased, pathological lesion and virus antigen in the tissues mitigated as compared with the controls. therapeutical dose of apg caused only slight deformation of the hepatic cells.19938105801
serological evidence of human hantavirus infection in argentina, bolivia and uruguay.a serological survey was conducted in 1985-1987 to determine the presence of infection for hantavirus in the general population in argentina, uruguay, paraguay and bolivia, as well as among rodent-exposed laboratory workers in argentina. out of 748 individuals tested by immunofluorescence 20 proved positive for hantaan virus 76/118 strain of whom 16 also reacted against seoul virus 80/39 strain and 2 against puumala virus sotkamo strain. ten out of 72 argentine laboratory workers were positive f ...19968734925
[study on the geographic distribution and serological typing of hfrs in hebei province].surveillance on the hfrs had been carried out during the period of 1986-1993. rodent density ranged from 4.6%-12.2% with r.norvegicus exceeding 60% in all kinds of rodents. hv antigen carrier rate of r.norvegicus was 1.3%-7.5%, much higher than that among other rodents. a significantly positive correlation had been observed between the hfrs morbidity and the r.norvegicus density. of 129 specimens from hfrs patients, 89% belonged to the type of seov. in the eastern and centre parts of hebei plain ...19968758402
[observational and experimental studies on relationship between domestic pigs and epidemic hemorrhagic fever investigation].observational and experimental studies on the relationship between domestic pigs and epidemic hemorrhagic fever (ehf) were carried out during 1986 to 1994 with reverse passive hemagglutination (rpha), reverse passive hemagglutination inhibition (rphi), horse-radish peroxidase-staphylococcal protein a (hrp-spa), indirect immunofluorescence assay (ifa) and reverse transcript polymerase chain reaction (rt-pcr) to understand the roles of domestic pigs played in the epidemic areas. results showed dom ...19958697938
[experimental study on the effect of qing-wen granule on epidemic hemorrhagic fever virus]. 19958732142
[study of cytopathogenic effect of epidemic hemorrhagic fever virus on normal human bone marrow cells].the cytopathogenic effect of epidemic hemorrhagic fever virus (ehfv) on cultured human bone marrow cells (hbc) was studied. specific viral antigen in hbc was detected at various times after ehfv infection with trypan-blue stain and electron microscopy. it was shown that the infection rate of hbc was over 80% at 3 weeks after infection. the death rate of hbc in the infection group was significantly higher than that in the control group on 1-4 weeks after infection. injury of cell membrane organs ...19938404319
evidence of hantavirus in wild rodents in northern ireland.a survey of evidence of rodent hantavirus infection in county down, northern ireland was carried out by using immunofluorescence to detect virus antigen and antibody. antibodies to hantavirus (r22 strain of seoul virus and hantaan 76-118) were found in 11/51 (21.6%) brown rats (rattus norvegicus), 1/31 (3.2%) field mice (apodemus sylvaticus) and 17/59 (28.8%) house mice (mus domesticus). seven rodents had evidence of hantavirus antigen in lung tissues. antibody positive animals were significantl ...19968870634
puumala and dobrava viruses cause hemorrhagic fever with renal syndrome in bosnia-herzegovina: evidence of highly cross-neutralizing antibody responses in early patient sera.hantavirus infection was diagnosed serologically by mu-capture igm and igg elisas in hemorrhagic fever with renal syndrome (hfrs) patients admitted to tuzla hospital, bosnia-herzegovina. the results indicated that more than one hantavirus caused the outbreak. to address the question of which hantavirus serotypes were involved, sequentially drawn sera were analyzed by focus reduction neutralization test (frnt) for antibodies against puumala, hantaan, dobrava, and seoul hantaviruses. the data reve ...19979298732
seroepidemiological survey of wild rats for seoul virus in indonesia.the prevalence of antibodies against seoul virus was investigated in 655 wild rats (rattus norvegicus, r. rattus, r. rattus diardii, r. exulans, r. tiomanicus) captured in seven port areas of indonesia. twenty-four of 238 r. norvegicus, one of 142 r. rattus and one of 102 r. exulans from two port areas had the antibodies against seoul virus.19968806040
immune responses to inactivated vaccine in people naturally infected with inactivated hantaan virus vaccine for hemorrhagic fever with renal syndrome (hfrs) was given by injection to 15 people who were naturally infected with either hantaan or seoul viruses. immunofluorescent antibody (ifa), reversed passive hemagglutination inhibition (rphi), hemagglutination inhibition (hi), and neutralization antibody (na) assays were used to measure the antibody titers of the vaccinated people before and after three doses of vaccine. the results indicated that ifa and rphi anti ...19968877768
urine-associated horizontal transmission of seoul virus among understand the mode of transmission of seoul type hantavirus in wistar rats, we examined the shedding of the virus and antibody production in infected rats. when 1-day-old rats were inoculated with the ki-83-262 strain of seoul virus, s segment of the viral genome was detected in lungs, clots, urine, saliva, submaxillary glands, rectums, and kidneys by nested reverse transcriptase pcr. on the other hand, when 8-week-old rats were infected with the virus, viral genome was detected only in the ...19989505963
modes of seoul virus infections: persistency in newborn rats and transiency in adult understand the mode of persistent infection of seoul virus in rodents, we examined the distribution of the virus genome and antibody production in infected rats. when 1-day-old rats were inoculated with the ki-83-262 strain, the s segment of viral genome was detected in sera, clots, lungs and kidneys from 3 to 184 days post inoculation (d.p.i.) by nested reverse transcriptase pcr. on the other hand, when 7-week-old rats were infected with this virus, viral genome was detected only in the lung ...19969526540
urine-associated horizontal transmission of seoul virus among understand the mode of transmission of seoul type hantavirus in wistar rats, we examined the shedding of the virus and antibody production in infected rats. when 1-day-old rats were inoculated with ki-83-262 strain of seoul virus, the s segment of the viral genome was detected in lungs, clots, urine, saliva, submaxillary glands, rectums, and kidneys by nested reverse transcriptase pcr. on the other hand, when 8-week-old rats were infected with the virus, viral genome was detected only in the ...19989541619
antigenic characterization of hantaan and seoul virus nucleocapsid proteins expressed by recombinant baculovirus: application of a truncated protein, lacking an antigenic region common to the two viruses, as a serotyping antigen.hantaan virus (htn) and seoul virus (seo) are members of the genus hantavirus in the family bunyaviridae and are causative agents of hemorrhagic fever with renal syndrome. the complete and truncated nucleocapsid proteins (np) of htn and seo were expressed by a recombinant baculovirus system. antigenic characterization of the np using monoclonal antibodies (mabs) indicated that the binding sites for the serotype-specific mabs were located between amino acids (aa) 155 and 429. a western blot assay ...19989705385
rapid and specific detection of sin nombre virus antibodies in patients with hantavirus pulmonary syndrome by a strip immunoblot assay suitable for field develop a rapid antibody test for sin nombre hantavirus (snv) infection for diagnosis of hantavirus pulmonary syndrome (hps) in field settings where advanced instrumentation is not available, a strip immunoblot assay bearing four immobilized antigens for snv and a recombinant nucleocapsid protein antigen of seoul hantavirus (seov) was prepared. the snv antigens included a full-length recombinant-expressed nucleocapsid (n) protein (rn), a recombinant-expressed g1 protein (residues 35 to 117), ...19979041397
prevalence of serum antibodies to hantaviruses in northern sweden as measured by recombinant nucleocapsid enzyme-linked immunosorbent assay (elisa) based on recombinant nucleocapsid protein (rn delta) (aa 1-117) of hantaan, seoul, dobrava, sin nombre and puumala hantaviruses was used to determine the prevalence of antibodies among randomized and stratified individuals from northern sweden. in total, 137/1533 individuals (8.9%) had specific serum igg antibodies to puumala virus, the only hantavirus known to occur in the region. the prevalence of antibodies to puumala virus (8.9%) was determined to ...19979360248
comparison of the protective efficacy of naked dna, dna-based sindbis replicon, and packaged sindbis replicon vectors expressing hantavirus structural genes in hamsters.seoul virus (seov) is a member of the hantavirus genus (family bunyaviridae) and an etiological agent of hemorrhagic fever with renal syndrome. the medium (m) and small (s) gene segments of seov encode the viral envelope glycoproteins and nucleocapsid protein, respectively. we compared the immunogenicity and protective efficacy of naked dna (pwrg7077), dna-based sindbis replicon (psin2.5), and packaged sindbis replicon vectors (psinrep5), containing either the m or s gene segment of seov in syri ...199910544095
hantavirus infection in scid mice.severe combined immunodeficiency (scid) mice were inoculated with hantaan virus strain 76-118 (htn) or seoul virus strain sr-11 (sr) of hantaviruses. susceptibility of scid mice was compared with those of immunocompetent adult mice, newborn mice and nude mice. scid mice inoculated with htn or sr died 32 to 35 days after infection. unlike newborn mice which also died of hantavirus infection, scid mice survived longer than newborn mice and showed typical wasting symptoms rather than nervous sympto ...19979362032
accumulation of terminally deleted rnas may play a role in seoul virus persistence.two independent, long-term infections were analyzed to determine whether changes in viral replication could contribute to the establishment and/or maintenance of persistent seoul virus infections. infected cell cultures initially contained high levels of infectious virus and intracellular viral rna that peaked between approximately 7 to 16 days postinfection and then gradually declined until day 26. after day 26, the viral titers and the levels of the small (s), medium (m), and large (l) viral r ...200010627543
seroprevalence study of hantavirus infection in the community based population.the genus hantavirus, family bunyaviridae, comprise at least 20 distinct viruses. ten of these have been linked to specific human diseases. hemorrhagic fever with renal syndrome has been recognized since the 1950s. hantavirus pulmonary syndrome was recognized in the united states in 1993. epizootiologic studies of norway rats in the baltimore area have shown that rodent infections with hantaviruses are common and geographically widespread with prevalence reaching 44% in tested rat populations. t ...199910628132
detection of hantaan and seoul viruses by reverse transcriptase-polymerase chain reaction (rt-pcr) and restriction fragment length polymorphism (rflp) in renal syndrome patients with hemorrhagic fever.hemorrhagic fever with renal syndrome (hfrs), also called korean hemorrhagic fever (khf), is the most common cause of acute renal failure in the far east. two serotypes of hantavirus, hantaan and seoul viruses are known pathogens for hfrs in korea.200010711409
[circulation of seoul hantavirus in populations of synanthropic rodents and its significance in the incidence of hemorrhagic fever with renal syndrome in the primorye territory].the prevalence of seoul virus in the primorie territory is presented and its significance in the morbidity structure with regard to hemorrhagic fever with the renal syndrome (hlps) is discussed. epidemiological and clinical manifestations of hlps caused by seoul virus are described. the authors conclude that in the urban foci of hlps it is associated mainly with seoul virus, while in the rural foci, with hantaan virus.199910544448
[studies on epidemic hemorrhagic fever among rats in jianghan plian, hubei province, china].capture of rats by digging their cavities, identification of rat strains, sex and age, examination of their skin cut or damage, and detection of antigen and antibody of epidemic hemorrhagic fever virus (ehfv) were conducted in epidemic areas of epidemic hemorrhagic fever (ehf) in jinaghan plain, hubei province, to study prevalence of ehfv infection in rats. results showed that apodemus agarius and rattus norvegius accounted for 69.7% and 23.6% of the total rats captured, respectively, their infe ...19979812588
dna vaccination with hantavirus m segment elicits neutralizing antibodies and protects against seoul virus infection.seoul virus (seov) is one of four known hantaviruses causing hemorrhagic fever with renal syndrome (hfrs). candidate naked dna vaccines for hfrs were constructed by subcloning cdna representing the medium (m; encoding the g1 and g2 glycoproteins) or small (s; encoding the nucleocapsid protein) genome segment of seov into the dna expression vector pwrg7077. we vaccinated balb/c mice with three doses of the m or s dna vaccine at 4-week intervals by either gene gun inoculation of the epidermis or n ...199910069952
truncated hantavirus nucleocapsid proteins for serotyping hantaan, seoul, and dobrava hantavirus infections.truncated recombinant nucleocapsid proteins (rnps) of hantaan virus (htnv), seoul virus (seov), and dobrava virus (dobv) were expressed by a baculovirus system. the truncated rnps, which lacked 49 (rnp50) or 154 (rnp155) n-terminal amino acids of the nps of htnv, seov, and dobv, were able to differentiate htnv-, seov-, and dobv-specific immune sera. recombinant np50s retained higher reactivities than rnp155s and were proven useful for enzyme-linked immunosorbent assay (elisa). the elisas based o ...200111427545
sex differences in immune responses and viral shedding following seoul virus infection in norway the field, male rodents are more frequently infected with hantaviruses than females. this study examined whether patterns of immune responses against hantavirus differed between the sexes. male and female long evans rats (rattus norvegicus) were inoculated with seoul virus, and antibody and cytokine responses, as well as virus shedding were assessed. males were more likely to shed virus in saliva, to shed virus through multiple routes (saliva, urine, and feces), and to have viral rna in the s ...200111504409
dna vaccination with the hantaan virus m gene protects hamsters against three of four hfrs hantaviruses and elicits a high-titer neutralizing antibody response in rhesus monkeys.four hantaviruses-hantaan virus (htnv), seoul virus (seov), dobrava virus (dobv) and puumala virus-are known to cause hemorrhagic fever with renal syndrome (hfrs) in europe and asia. htnv causes the most severe form of hfrs (5 to 15% case-fatality rate) and afflicts tens of thousands of people annually. previously, we demonstrated that dna vaccination with a plasmid expressing the seov m gene elicited neutralizing antibodies and protected hamsters against infection with seov and htnv. here, we r ...200111507192
sex differences in seoul virus infection are not related to adult sex steroid concentrations in norway rats.field studies of hantavirus infection in rodents report that a higher percentage of infected individuals are males than females. to determine whether males were more susceptible to hantavirus infection than females, adult male and female long evans rats (rattus norvegicus) were inoculated with doses of seoul virus ranging from 10(-4) to 10(6) pfu. the 50% infective doses (id(50)) were not significantly different for male and female rats (10(0.05) and 10(0.8) pfu, respectively). to determine whet ...200010933735
genetic diversity of hantaviruses associated with hemorrhagic fever with renal syndrome in the far east of identify the hantaviruses causing hemorrhagic fever with renal syndrome (hfrs) in the far east of russia, blood samples collected from hfrs patients in 1994-1998, were examined by reverse transcription-polymerase chain reaction. in addition, 36 sera were tested by an immunofluorescence assay for antibodies against hantaan, seoul, puumala, and khabarovsk viruses, and 54 samples were tested by plaque reduction neutralization test. with both serological assays, the highest antibody titers were t ...200011074123
detection of hantaviral antibodies among patients with hepatitis of unknown etiology in japan.hantaviral antibodies were detected in the sera from patients with hepatic disease of unknown etiology in japan by several different serological diagnostic methods. a total of 105 sera from diseased patients which were negative to a-g hepatitis virus infections in the tokyo area were tested. among them, 3 out of 73 sera from patients with chronic hepatic disease were positive to hantaviral antibody by enzyme-linked immunosorbent assay (elisa), indirect immunofluorescent antibody assay (ifa) and ...200010888353
[a preliminary genetic reassortment between hantaan virus and seoul virus strains].to determine the frequency and characteristics of reassortment among hantaan and seoul viruses causing hemorrhagic fever with renal syndrome (hfrs).200212015110
a dominant antigenic region of the hantaan virus nucleocapsid protein is located within a amino-terminal short stretch of hydrophilic residues.the nucleocapsid (n) protein of the hantaan virus (htnv) is a major viral antigen that induces a strong antibody response during the acute phase of infection. by immunoblot analyses of the recombinant n proteins using human sera of the hemorrhagic fever with renal syndrome (hfrs), we have confirmed previous finding by other investigators of the presence of a highly antigenic region near the amino terminus of the htnv n protein. we have further located the antigenic region within a short stretch ...200111724272
generation of an hfrs patient-derived neutralizing recombinant antibody to hantaan virus g1 protein and definition of the neutralizing domain.hantaan virus (htnv) in the hantavirus genus, family bunyaviridae, is the major cause of severe hemorrhagic fever with renal syndrome (hfrs). we prepared a combinatorial phage display library of human fabs to htnv from rna extracted from the blood lymphocytes of a convalescent hfrs patient. we selected two g1 glycoprotein-specific clones and one nucleocapsid protein (n)-specific clone from the fab library for further studies. the human fab antibodies were converted to igg form in baculovirus/ins ...200312436484
serological study of hantavirus in man in the autonomous community of madrid, relating to hantavirus infection in spain are scarce and limited to rural areas. the aim of this work was to study the seroprevalence of hantavirus infection in the autonomous community of madrid (acm), a region containing both rural and urban populations in different ecological settings. sera from 3852 individuals (1849 male, 2003 female) were screened by indirect inmunofluorescence, with vero e6 cells infected with puumala, hantaan and seoul viruses as antigens. screen-positive results we ...200212435066
[detection of hfrsv in eulaelaps shanghaiensis and ornithonyssus bacoti by using in situ hybridization].to provide molecular biological evidence of transmission of hemorrhogic fever with renal syndrome virus (hfrsv) by gamasid mites, eulaelaps shanghaiensis and ornithonyssus bacoti.199812078290
the multimerization of hantavirus nucleocapsid protein depends on type-specific epitopes.multimerization of the hantaan virus nucleocapsid protein (np) in hantaan virus-infected vero e6 cells was observed in a competitive enzyme-linked immunosorbent assay (elisa). recombinant and truncated nps of hantaan, seoul, and dobrava viruses lacking the n-terminal 49 amino acids were also detected as multimers. although truncated nps of hantaan virus lacking the n-terminal 154 amino acids existed as a monomer, those of seoul and dobrava formed multimers. the multimerized truncated np antigens ...200312502810
use of vesicular stomatitis virus pseudotypes bearing hantaan or seoul virus envelope proteins in a rapid and safe neutralization test.a vesicular stomatitis virus (vsv) pseudotype bearing hantavirus envelope glycoproteins was produced and used in a neutralization test as a substitute for native hantavirus. the recombinant vsv, in which the enveloped protein gene (g) was replaced by the green fluorescent protein gene and complemented with g protein expressed in trans (vsvdeltag*g), was kindly provided by m. a. whitt. 293t cells were transfected with plasmids for the expression of envelope glycoproteins (g1 and g2) of htnv or se ...200312522053
[studies on the experimental transmission of rattus-borne hantavirus by ornithonyssus bacoti].to demonstrate the role of rat mite (ornithonyssus bacoti) in the transmission of rattus-borne hemorrhagic fever with renal syndrome (hfrs).199812078291
neonatal sex steroids affect responses to seoul virus infection in male but not female norway rats.previous studies illustrate that after inoculation with seoul virus (i.e., the naturally occurring hantavirus found in norway rats), adult male rats produce higher antibody responses, exhibit higher th1 responses (i.e., igg2a, il-2, and ifn gamma), and shed virus longer than females, but these difference are not altered by manipulation of sex steroids in adulthood. to determine whether sex steroid hormones organize adult responses to hantavirus infection, at 2-4 days of age, male rats were gonad ...200212480503
prevalence study of antibody to ratborne pathogens and other agents among patients using a free clinic in downtown los angeles.norway rats (rattus norvegicus) are hosts for various microbes. homeless people who have contact with rats may be at risk of infection by them. the los angeles county department of health services initiated a seroepidemiologic study among patients who used a free clinic in downtown los angeles; 200 serum specimens obtained for other routine assays were tested for antibodies to ratborne pathogens and other agents. the seroprevalence of antibody to hepatitis e virus in this population was 13.6%; t ...200212447746
[passage adaptability of candidate strains for hemorrhagic fever with renal syndrome purified vaccine in vero cells and their immunogenicity].to adapt the candidate strains of hemorrhagic fever with renal syndrome (hfrs) purified vaccine to vero cells and to study their antigenicity and immunogenicity.200312869992
serological analysis of hemorrhagic fever with renal syndrome (hfrs) patients in far eastern russia and identification of the causative hantavirus genotype.hemorrhagic fever with renal syndrome (hfrs) is endemic in east asia and europe. the disease is caused by several viruses belonging to the genus hantavirus, including the hantaan virus (htnv), seoul virus (seov), dobrava belgrade virus (dobv), and puumala virus (puuv). recently, htnv-related viruses, amur (amr) and far east (fe) genotypes were identified as causative agents of hfrs in far eastern russia. to investigate the epidemiology of hfrs and virus transmission, we collected sera from 17 ac ...200312898330
study on biologic activity for membrane of normal bone marrow cells with infection of epidemic hemorrhagic fever virus.using dph fluorescence probe, the membrane of normal bone marrow cells with infection of epidemic hemorrhagic fever virus (ehfv) was labeled. the membrane lipid fluidity was obviously decreased from the membrane lipid fluorescence polarization. the membrane lipid fluidity of lymphocyte, monocyte and neutrophilic granulocyte was dynamically observed. after culturing the cells for 1, 6, 24 and 72 h, it was found that all the membrane lipid fluidity of the infected cells was decreased obviously wit ...200012840924
evidence of the presence of seoul virus in cambodia.a study was conducted in agricultural and urban areas in cambodia to assess the presence of hantaviruses in rodent populations. in 1998, rodents were trapped in two villages and in phnom penh city around market places and a rubbish dump. igg antibodies to hantaan virus were detected in 54 (8.2%) rodents among 660 tested: 6.4% (13/203) among roof rats (rattus rattus), 20.9% (39/187) among norway rats (r. norvegicus), 16.7% (2/12) among unidentified rattus species and none in 183 polynesian rats ( ...200312850202
[antigenic and genetic properties of seoul virus strains--a causative agent of hemorrhagic fever with renal syndrome].data on the circulation of a new genetic variant of seol virus, which was designated as "vladivostok" (the name of the place where it was isolated)--vdv, were obtained, by using the antigenic and genetic analysis of hantavirus strains isolated from patients with hemorrhagic fever with renal syndrome (hfrs) and from brown rats, and described in the present paper. a complete identity of nucleotide sequences of the m-segment fragment of isolates obtained from hfrs patients and of those obtained fro ...200312894473
[considerations on the discovery of the epidemic hemorrhagic fever virus in rodents of epidemic and nonepidemic areas of the province of buenos aires]. 195914440122
prevalence of rabies virus and hantaan virus infections in commensal rodents and shrews trapped in bangkok.five hundred rodents and shrews (rattus norvegicus: 458, rattus rattus: 28, rattus exulans: 5, mus musculus: 4 and suncus murine: 5) trapped from the fresh food markets around bangkok area were investigated for rabies virus and hantaan virus infections. no rabies viral antigens in the animals' brains were detected by direct immunofluorescence. on the other hand, antibodies to hantaan virus were demonstrated in the sera of 7 (1.53%) r. norvegicus caught in various markets using a particle aggluti ...200314696782
synthesis of seoul virus rna and structural proteins in cultured cells.seoul virus is a hantavirus that causes hemorrhagic fever with renal syndrome (hfrs). the virion has a tripartite (s, m, and l) negative-stranded rna genome, which is characteristic of the family bunyaviridae. however, the molecular basis of virus replication is not well known. we established a northern blot hybridization (nb) procedure using digoxygenin-labeled rna probes, to quantitate the hantaviral plus- and minus-strand rnas separately. virus rna replication was analyzed in infected vero e6 ...200314505081
wounding: the primary mode of seoul virus transmission among male norway rodent populations, males are more likely to be infected with hantaviruses and to engage in aggression than are females. to assess the relationship between aggression and seoul virus infection, norway rats were trapped in baltimore, maryland and wounding, infection status, and steroid hormone concentrations were examined. older males and males with high-grade wounds were more likely to have igg antibody to seoul, to shed virus in saliva, urine, and feces, and to have viral rna in organs than ...200415031523
microarray technology for identification and distinction of hantaviruses.dna microarrays combine high-precision technology with advanced molecular biology to achieve high-throughput screening of dna fragments. in this study, we investigated the potential of the cdna microarray technique to identify and discriminate pcr derived amplicons from genetically highly similar viruses. the wide range of sequence variation among hantaviruses makes them suitable as a model for this purpose. the hantaviruses, carried by rodents, cause several hundred thousand cases of severe hum ...200414981768
association of the nucleocapsid protein of the seoul and hantaan hantaviruses with small ubiquitin-like modifier-1-related molecules.we performed yeast two-hybrid screening of a human kidney cell cdna library to study the biological role of the hantavirus nucleocapsid protein (np). we found that seoul virus (seov) and hantaan virus (htnv) nps were associated with small ubiquitin-like modifier (sumo)-1-interacting proteins pias1, piasxbeta, hipk2, chd3, and ttrap, which interacted with the sumo-1 conjugating enzyme (ubc-9) and sumo-1 in the yeast two-hybrid assay. interactions between the hipk2, chd3, and ttrap proteins and se ...200314609633
[voluntary inoculation of the epidemic hemorrhagic fever virus; clinical & etiological study]. 195913639773
persistent seoul virus infection in lewis rats.mechanistic studies of hantavirus persistence in rodent reservoirs have been limited by the lack of a versatile animal model. this report describes findings from experimental infection of inbred lewis rats with seoul virus strain 80-39. rats inoculated with virus intraperitoneally at 6 days of age became persistently infected without clinical signs. tissues from seoul virus-inoculated 6-day-old rats were assessed at 6, 9, and 12 weeks post-inoculation for viral rna by rt-pcr and in situ hybridiz ...200415221534
seoul hantavirus infection mimicking dengue fever. 200315190673
[seroprevalence of antibodies to seoul virus in occupational risk groups].a seroprevalence study was performed to determine the percentage of people exposed to seoul virus among biologists and police officers (n = 226), whose work might put them into contact with viral reservoirs. detection of specific igg antibodies was performed by indirect immunofluorescence and western-blot. there was one positive result among the biologists (4.54%). this study confirms seoul virus exposure in one of the employment groups studied.200415056437
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