TitleAbstractYear(sorted ascending)
ten-year prognosis of puumala hantavirus-induced acute interstitial nephritis.nephropathia epidemica (ne) is a hemorrhagic fever with renal syndrome caused by puumala hantavirus. its long-term prognosis is considered favorable. there are, however, some reports about subsequent hypertension, glomerular hyperfiltration, and proteinuria after previous hantavirus infection. therefore, we studied 36 patients 5 and 10 years after acute ne, with 29 seronegative controls. office blood pressure, ambulatory 24-h blood pressure (abp), glomerular filtration rate (gfr), and proteinuri ...200616641933
a new puumala hantavirus subtype in rodents associated with an outbreak of nephropathia epidemica in south-east germany in 2004.a micro-epidemic of hantavirus infections occurred in lower bavaria, south-east germany, starting in april 2004. while only three cases were registered from 2001 to 2003, a dramatically increased number of clinically apparent human hantavirus infections (n=38) was observed in 2004, plus seven additional cases by june 2005. to determine the reservoir responsible for the infections, a total of 43 rodents were trapped in lower bavaria. serological and genetic investigations revealed that puumala vi ...200616650330
is there an increased long-term risk of hypertension and renal impairment after puumala virus-induced nephropathy?nephropathia epidemica (ne) is a mild form of hemorrhagic fever with renal syndrome, assumed to have a favorable prognosis. ne patients who manifested a higher glomerular filtration rate and mean systolic blood pressure, and more proteinuria, versus controls at 5 years of follow-up demonstrated no major abnormalities after 10 years. antihypertensive treatment was, however, more common. could ne predispose some patients to develop hypertension after all?200616724089
genetic analysis of puumala hantavirus strains from austria.sequences of the small (s) and the medium (m) genome segments of puumala hantavirus (puuv) were recovered from bank voles clethrionomys glareolus trapped at 2 locations, klippitztörl (carinthia) and ernstbrunn (lower austria). lung tissue samples from 12 rodents earlier found hantavirus antibody-positive were further screened for the presence of hantaviral n-antigen using immunoblotting. rna purified from 7 n-ag-positive samples was subjected to the reverse transcription-polymerase chain reactio ...200616798703
prolonged survival of puumala hantavirus outside the host: evidence for indirect transmission via the environment.the capability of rodent-borne viruses to survive outside the host is critical for the transmission dynamics within rodent populations and to humans. the transmission of puumala virus (puuv) in colonized bank voles (clethrionomys glareolus) was investigated and additional longevity studies in cell culture with puuv and tula (tulv) hantaviruses were performed. wild-type puuv excreted by experimentally infected donor bank voles was shown to be transmitted indirectly between rodents through contami ...200616847107
loss of cell membrane integrity in puumala hantavirus-infected patients correlates with levels of epithelial cell apoptosis and perforin.hemorrhagic fever with renal syndrome and hantavirus pulmonary syndrome are two diseases caused by hantaviruses. capillary leakage is a hallmark of hantavirus infection. pathogenic hantaviruses are not cytotoxic, but elevated levels of serum lactate dehydrogenase (ldh), indicative of cellular damage, are observed in patients. we report increased levels of serum perforin, granzyme b, and the epithelial cell apoptosis marker caspase-cleaved cytokeratin-18 during puumala hantavirus infection. signi ...200616873286
regions of importance for interaction of puumala virus nucleocapsid subunits.puumala virus (puuv) is a hantavirus that causes a mild form of hemorrhagic fever with renal syndrome in northern and central europe, and in large parts of russia. the nucleocapsid (n) protein encoded by hantaviruses plays an important role in the life-cycle of these viruses, and one important function for the n-protein is to oligomerize, surround and protect the viral rnas. we have identified amino- and carboxy-terminal regions involved in puuv n-n interactions, which comprise amino acids 100-1 ...200616972031
characterization of a new puumala virus genotype associated with hemorrhagic fever with renal syndrome.hantavirus serotype puumala (puuv) is the etiologic agent of nephropathia epidemica (ne), a mild variant of hemorrhagic fever with renal syndrome (hfrs) in europe, with lethality rates up to 1% among human patients. the serotype puuv is composed of numerous geographically restricted strains forming a great number of phylogeographic lineages, sublineages, variants, and clusters. we describe a new, geographically and phylogenetically well-defined pathogenic puuv sublineage in southwest germany (st ...200616972039
hantavirus and arenavirus antibody prevalence in rodents and humans in trentino, northern italy.the spatial and temporal distribution of hantavirus and arenavirus antibody-positive wild rodents in trentino, italy, was studied using immunofluorescence assays (ifa) in two long-term sites trapped in 2000-2003, and six other sites trapped in 2002. the overall hantavirus seroprevalence in the bank voles, clethrionomys glareolus (n=229) screened for puumala virus (puuv) antibodies was 0.4%, and that for apodemus flavicollis mice (n=1416) screened for dobrava virus (dobv) antibodies was 0.2%. ant ...200616371172
maternal antibodies postpone hantavirus infection and enhance individual breeding success.the transfer of maternal antibodies from mother to progeny is a well-known phenomenon in avian and mammalian species. optimally, they protect the newborn against the pathogens in the environment. the effect of maternal antibodies on microparasite transmission dynamics may have important consequences for both the fitness of the host and the epizootic processes of the pathogens. however, there is a scarcity of studies examining these effects in free-living wild species. we studied the influence of ...200617015326
development of novel immunoglobulin g (igg), iga, and igm enzyme immunoassays based on recombinant puumala and dobrava hantavirus nucleocapsid proteins.human infections with asian and european hantaviruses can result in hemorrhagic fever with renal syndromes of differing severities characterized by renal dysfunction and sometimes by pulmonary symptoms. for the serological detection of human infections by hantaviruses relevant for europe, we developed monoclonal antibody capture immunoglobulin g (igg) and iga enzyme-linked immunosorbent assays (elisas) based on yeast-expressed nucleocapsid proteins of puumala and dobrava hantaviruses. moreover, ...200617021245
mild course of puumala nephropathy in children in an area with sporadic occurrence hantavirus infection.the first three children with puumala virus nephropathy diagnosis in the czech republic are reported on. a boy and two girls were admitted with symptoms of interstitial nephritis. the medical history in all children revealed flu-like symptoms. all patients were mildly pyrexial and had elevated erythrocytes sedimentation rate, c-reactive protein and low hemoglobin levels. serum creatinine levels were elevated and proteinuria exceeded 700 mg/l in all children. tubular proteinuria, glycosuria, high ...200617024393
dynamics of puumala virus infection in bank voles in ardennes department (france).the hantaviruses (genus hantavirus, family bunyaviridae) include human pathogens and occur worldwide. in western and central europe, the predominant serotype is puumala (puu) virus, which causes epidemic nephropathy. voles are considered to be the main reservoir and the vector of puu virus. a total of 719 rodents (mainly clethrionomys glareolus, apodemus sp.) trapped by capture-mark-recapture (cmr) in four sites in ardennes department (france) between april 2004 and october 2005 were tested for ...200617027178
puumala virus rna in patient with multiorgan failure. 200617080583
[epizootology of hemorrhagic fever with renal syndrome in the central chernozem region].a total of 5149 small mammals belonging to 16 species were collected from the lipetsk, voronezh, and belgorod regions (40 administrative districts) in 2003-2004 and examined by elisa and ifa to detect hantavirus antigen and antibodies in the lung tissues. hantavirus circulation was revealed in 13 species, the highest hantavirus activity being detected in field (apodemus agrarius) and small wood (a. (s) uralensis) mice (dobrava-belgrad virus), bank (clethrionomis glareolus) (puumala virus) and co ...200617087062
serological survey for viral pathogens in turkish rodents.wild rodents (n = 330) were trapped around the villages of altindere and coşandere (maçka, trabzon province), ayder, ortan, and yolkiyi (camlihemşin, rize province), and bozdag (odemiş, izmir province) in northeastern and western turkey during april 2004. samples were tested for arenavirus, hantavirus, and cowpox virus (family poxviridae, genus orthopoxvirus, cpxv) antibodies by using immunofluorescence assays (ifas). antibodies against arenaviruses were found in eight of 330 (2.4%) rodents. are ...200617092901
tumor necrosis factor-alpha genetic predisposing factors can influence clinical severity in nephropathia epidemica.severe human infection with hantavirus is characterized by high fever, cold chills, thrombocytopenia, arterial hypotension, acute renal failure, and/or adult respiratory distress syndrome (ards)-like pulmonary involvement, but the clinical course varies greatly between individuals. we investigated whether genetically determined differences in tumor necrosis factor (tnf)-alpha production can influence the severity of hantavirus disease. we studied a tnf-alpha single-nucleotide promoter polymorphi ...200616987073
puumala hantavirus in slovenia: analyses of s and m segment sequences recovered from patients and slovenia, the co-existence of dobrava and puumala (puuv) hantaviruses in a single endemic region has been demonstrated. this study presents selected slovenian hfrs cases caused by puuv combined with genetic analysis of viral genome sequences recovered from clinical specimens and tissue samples of clethrionomys glareolus (bank voles). serum samples from nine hfrs patients were included in the study. rodents study sites were selected with regard to the hfrs cases. partial s segment sequences we ...200716997412
environmental conditions and puumala virus transmission in belgium.non-vector-borne zoonoses such as puumala hantavirus (puuv) can be transmitted directly, by physical contact between infected and susceptible hosts, or indirectly, with the environment as an intermediate. the objective of this study is to better understand the causal link between environmental features and puuv prevalence in bank vole population in belgium, and hence with transmission risk to humans. our hypothesis was that environmental conditions controlling the direct and indirect transmissio ...200718078526
characterization of hemorrhagic fever with renal syndrome caused by hantaviruses, estonia.thirty cases of hemorrhagic fever with renal syndrome (hfrs) due to puumala virus (puuv), saaremaa virus (saav), and dobrava virus infection were confirmed in estonia. except for the levels of serum creatinine, no remarkable differences were found in the clinical course of hfrs caused by puuv and saav.200718217569
[the role of natural environment in spreading of hantavirus--model of the correlation between host, pathogen and human infections].the environmental changes caused by humans influence ecosystem and thus have significant impact on occurrence of emerging and re-emerging diseases. the hantavirus infection belong to the one of them. the aim of this paper was to present current knowledge about relationship between hantavirus, their natural host and the spread of the infection to people. rodents constitute both the natural host of the hantaviruses and the reservoir of hantavirus for environment. circulation of the virus in the ro ...200718572496
cross-reactive immune responses in mice after genetic vaccination with cdna encoding hantavirus nucleocapsid proteins.hantaviruses cause hemorrhagic fever with renal syndrome (hfrs) in about 150,000 individuals in eurasia, and several hundred cases of hantavirus pulmonary syndrome (hps) on the american continent annually. there is consequently a need for rapid diagnostics and effective prevention of hantaviral infections. in this study we have performed dna-vaccination of mice with full-length genes encoding the immunogenic nucleocapsid protein (np) of puumala (puuv), seoul (seov) and sin nombre virus (snv). th ...200717110000
serological evidence for borna disease virus infection in humans, wild rodents and other vertebrates in finland.borna disease virus (bdv) can infect many vertebrate species, including humans. bdv infection may lead to meningoencephalomyelitis in animals. an association with human neuropsychiatric diseases has been reported, but the causal relationship between bdv and human disease remains unclear.200717129759
development of serological assays for thottapalayam virus, an insectivore-borne hantavirus.thottapalayam virus (tpmv), a member of the genus hantavirus in the family bunyaviridae, was isolated from an insectivore, suncus murinus (musk shrew), captured in southern india in 1964. while the isolation of tpmv predates the discovery of the prototype hantaan virus, little is known about its genetics and biology. to date, preliminary evidence suggests that tpmv differs significantly, both antigenically and genetically, from all known rodent-borne hantaviruses. however, since detailed epizoot ...200717182762
evaluation of the efficacy of disinfectants against puumala hantavirus by real-time rt-pcr.puumala virus, a hantavirus belonging to the bunyaviridae family, causes a human disease known as nephropathia epidemica, a mild form of hemorrhagic fever with renal syndrome. the implementation of effective decontamination procedures is critical in hantavirus research to minimize the risk of personnel exposure. this study investigated the efficacy of clidox((r)), dettol((r)), ethanol, halamid-d((r)), peracetic acid, sodium hypochloride and virkon((r))s for inactivating puumala virus. a real-tim ...200717188760
radiological findings and their clinical correlations in nephropathia epidemica.nephropathia epidemica (ne) is a mild form of hemorrhagic fever with renal syndrome (hfrs). its course varies from asymptomatic to fatal. the etiologic agent, puumala virus, belongs to the hantavirus genus of the bunyaviridae family. respiratory symptoms, from common cold to respiratory distress, occur in ne. acute renal failure (arf) is evident in over 90% of hospital-treated ne patients. in this review article, special attention is paid to radiological lung and renal involvement to investigate ...200717453509
determinants of the geographic distribution of puumala virus and lyme borreliosis infections in belgium.vector-borne and zoonotic diseases generally display clear spatial patterns due to different space-dependent factors. land cover and land use influence disease transmission by controlling both the spatial distribution of vectors or hosts, and the probability of contact with susceptible human populations. the objective of this study was to combine environmental and socio-economic factors to explain the spatial distribution of two emerging human diseases in belgium, puumala virus (puuv) and lyme b ...200717474974
[study of the phylogenetic relations of puumala hantaviruses, by using the mutual information calculating method].phylogenetic relations were investigated in the group of puumala viruses (the genus hantavirus, the family bunyaviridae) by the mutual information showing the similarity of the nucleotide sequences of s- and m-genome segments. there was evidence that recombination events might occurr in some eurasian puumala viruses (scandinavian and west-siberian genotypes). the investigation revealed the conservative sites available in the s and m segments that were common to most puumala viruses, as well as t ...200717500234
lambda interferon (ifn-lambda) in serum is decreased in hantavirus-infected patients, and in vitro-established infection is insensitive to treatment with all ifns and inhibits ifn-gamma-induced nitric oxide production.hantaviruses, causing hemorrhagic fever with renal syndrome (hfrs) and hantavirus cardiopulmonary syndrome (hcps), are known to be sensitive to nitric oxide (no) and to pretreatment with type i and ii interferons (alpha interferon [ifn-alpha]/ifn-beta and ifn-gamma, respectively). elevated serum levels of no and ifn-gamma have been observed in hfrs patients, but little is known regarding the systemic levels of other ifns and the possible effects of hantaviruses on innate antiviral immune respons ...200717522204
puumala and tula hantaviruses in france.the first genome sequences of tula (tulv) and puumala (puuv) hantaviruses undoubtedly originated from france were recovered from tissue samples of european common voles and bank voles captured in jura region. genetic analysis of s and m segments of french puuv strain revealed its highest similarity to strains from neighboring belgium and germany and also from slovakia. on phylogenetic trees, french puuv strain was placed within the central european lineage formed by strains from these three coun ...200717532080
puumala hantavirus viremia diagnosed by real-time reverse transcriptase pcr using samples from patients with hemorrhagic fever and renal syndrome.puumala virus (puuv) is the endemic hantavirus in northern sweden and causes nephropathia epidemica (ne), a milder form of hemorrhagic fever with renal syndrome. there is a need for fast and reliable diagnostics to differentiate the disease from other infections. by aligning virus rna sequences isolated from 11 different bank voles and one human patient, we designed a real-time reverse transcriptase (rt) pcr method for detection of puuv rna. the real-time rt-pcr assay showed linearity from 20 to ...200717537944
[prevalence of antibodies to hantavirus among hemodialysis patients with end-stage renal failure in kaunas and its district].the objective of this study was to investigate the prevalence of antibodies to hantaviruses among hemodialysis patients with end-stage renal failure in kaunas and its district.200717551280
detection of phylogenetically distinct puumala-like viruses from red-grey vole clethrionomys rufocanus in order to investigate whether puumala virus (puuv) or puuv-like virus is present in china, clethrionomys rufocanus and c. rutilus were captured in the jilin province during the spring and autumn of 2002-2003 for detection of puuv viral rna by rt-pcr and confirmation of puuv-positive antigens by an immunofluorescence assay. puuv-positive rna was identified in six out of 121 c. rufocanus but not in any of the 41 c. rutilus. complete s and partial m sequences (nt 1,316-1,598 and 2,687-3,089) were ...200717596824
hantavirus disease outbreak in germany: limitations of routine serological diagnostics and clustering of virus sequences of human and rodent europe, hemorrhagic fever with renal syndrome results mainly from infection with puumala virus (puuv) or dobrava virus. for 31 patients from a hantavirus disease outbreak in lower bavaria, a district in southeast germany, serodiagnosis was undertaken by enzyme-linked immunosorbent assay, immunofluorescence assay, and immunoblot analysis. in a few of these cases, however, puuv-specific typing of antibodies by these standard assays failed and a virus neutralization assay under biosafety level 3 ...200717626170
human cd8+ t cell memory generation in puumala hantavirus infection occurs after the acute phase and is associated with boosting of ebv-specific cd8+ memory t cells.the induction and maintenance of t cell memory is incompletely understood, especially in humans. we have studied the t cell response and the generation of memory during acute infection by the puumala virus (puuv), a hantavirus endemic to europe. it causes a self-limiting infection with no viral persistence, manifesting as hemorrhagic fever with renal syndrome. hla tetramer staining of pbmc showed that the cd8(+) t cell response peaked at the onset of the clinical disease and decreased within the ...200717641066
[transmission by feces of infected rodents. hantavirus illnesses are increasing]. 200717672003
[the evaluation of viral load in patients with hemorrhagic fever with renal syndrome (hfrs) by real-time polymerase chain reaction].the system for real-time polymerase chain reaction was developed for detection of puumala virus rnaand quantitative analysis of viral rnaload in clinical samples. dynamics of viral load in blood samples from 12 patients with hfrs (from day 4 to day 17 of the disease) was studied. at day 4 viral rna load varied from 4 x 10(4) to 1 x 10(6) copies/ml. during the period of observation viral load decreased by 10 - 200 times and was not detected in majority of patients to day 11 - 15 of the disease. t ...200717672134
prevalence of anti-hantavirus antibodies in patients with hypertransaminemia in madrid (spain).the hantaviruses are involved in a number of clinical syndromes of different severity and prognosis. hantaviruses are widely distributed around the world, but the spectrum of illnesses they cause outside recognized endemic areas is unclear. a retrospective analysis was performed to detect anti-hantavirus antibodies in the serum of patients with hypertransaminemia of unknown etiology and in that of healthy members of the general population of madrid (spain). antibodies were detected by indirect i ...200717690415
tula and puumala hantavirus nss orfs are functional and the products inhibit activation of the interferon-beta promoter.the s rna genome segment of hantaviruses carried by arvicolinae and sigmodontinae rodents encodes the nucleocapsid (n) protein and has an overlapping (+1) open reading frame (orf) for a putative nonstructural protein (nss). the aim of this study was to determine whether the orf is functional. a protein corresponding to the predicted size of tula virus (tulv) nss was detected using coupled in vitro transcription and translation from a cloned s segment cdna, and a protein corresponding to the pred ...200717705180
endemic hantavirus infection impairs the winter survival of its rodent host.the influence of pathogens on host fitness is one of the key questions in infection ecology. hantaviruses have coevolved with their hosts and are generally thought to have little or no effect on host survival or reproduction. we examined the effect of puumala virus (puuv) infection on the winter survival of bank voles (myodes glareolus), the host of this virus. the data were collected by monitoring 22 islands over three consecutive winters (a total of 55 island populations) in an endemic area of ...200717824420
genetic evidence of puumala and tula hantaviruses in rodents in the jura region, france--preliminary results. 200717868570
[identification of a new puumala hantavirus subtype in rodents from china].in order to detect the nucleic acid of puumala hantavirus, rna was extracted from lungs of bank voles captured in northeast china, and partial s and m genome segments of puumala virus were amplified by rt-pcr and sequenced. phylogenetic analysis suggested that chinese puumala virus had diverged from the common node of puuv, with accumulating nucleotide substitutions and formed a distinct lineage from other puumala viruses. newly found puumala virus was most closely related to the kamiiso-8cr-95 ...200717894236
puumala virus rna in cerebrospinal fluid in a patient with uncomplicated nephropathia epidemica. 200717900974
muju virus, a novel hantavirus harboured by the arvicolid rodent myodes regulus in korea.acute-phase sera from >5 % of cases of haemorrhagic fever with renal syndrome occurring annually in korea have been found to exhibit a fourfold or higher antibody titre to puumala virus (puuv) than to hantaan virus (htnv) by double-sandwich igm elisa, suggesting the existence of a puuv-related hantavirus. based on the phylogenetic relationships among arvicolid rodents, the royal vole (myodes regulus) was targeted as a likely reservoir host of hantavirus. using rt-pcr, a genetically distinct hant ...200717947538
[the cross and unspecific reactions in serological examination for antibodies against hantavirus puumala].a serological survey of 78 zoologist capturing small wild rodents in their environment, 9 patients with suspected hantavirus infections and 21 patients with acute renal dysfunction for antibodies to hantaviruses was conducted in poland. survey was done by the indirect elisa with puumala and hantaan virus antigens. out of the 78 mammalogists 15 were seropositive for hantavirus puumala igg without history of clinical illness. analysis of relation between reactive zoologist's sera igg with antigens ...200718069385
[occurrence of human hantavirus infections in poland].a spread of hantavirus infections causing hemorrhagic fever with renal syndrome is one of the rising epidemiological problems in the world. there is evidence that this type of viruses exists in almost all european countries. it is known that in poland all the species of wild rodents hosting european hantaviruses are present. however, the data on hantavirus are very limited and information on incidence and prevalence of hantavirus infections among humans and animals in our country is lacking. up ...200718069386
increased permeability of human endothelial cell line ea.hy926 induced by hantavirus-specific cytotoxic t lymphocytes.hantavirus infection causes two human diseases, hemorrhagic fever with renal syndrome and hantavirus pulmonary syndrome. the typical feature of these diseases is increased permeability in microvascular beds in the kidneys and the lungs, respectively. the mechanism of capillary leakage, however, is not understood. some evidence suggests that hantavirus disease pathogenesis is immunologically mediated by cytotoxic t lymphocytes and other immune cells in target organs producing inflammatory cytokin ...200716979772
kinship, dispersal and hantavirus transmission in bank and common voles.hantaviruses are among the main emerging infectious agents in europe. their mode of transmission in natura is still not well known. in particular, social features and behaviours could be crucial for understanding the persistence and the spread of hantaviruses in rodent populations. here, we investigated the importance of kinclustering and dispersal in hantavirus transmission by combining a fine-scale spatiotemporal survey (4 km2) and a population genetics approach. two specific host-hantavirus s ...200818071626
association between the dqa mhc class ii gene and puumala virus infection in myodes glareolus, the bank vole.puumala virus (puuv) is a hantavirus specifically harboured by the bank vole, myodes (earlier clethrionomys) glareolus. it causes a mild form of hemorrhagic fever with renal syndrome (hfrs) in humans, called nephropathia epidemica (ne). the clinical severity of ne is variable among patients and depends on their major histocompatibility complex (mhc) genetic background. in this study we investigated the potential role of class ii mhc gene polymorphism in the susceptibility/resistance to puuv in t ...200817693139
[detection of anti-hantavirus antibodies in forest workers in the north-east of poland].to estimate exposition to hantaviruses in a high-risk population (forest workers) in the north-east of poland.200819108515
a protein that replaces the entire cellular eif4f complex.the eif4f cap-binding complex mediates the initiation of cellular mrna translation. eif4f is composed of eif4e, which binds to the mrna cap, eif4g, which indirectly links the mrna cap with the 43s pre-initiation complex, and eif4a, which is a helicase necessary for initiation. viral nucleocapsid proteins (n) function in both genome replication and rna encapsidation. surprisingly, we find that hantavirus n has multiple intrinsic activities that mimic and substitute for each of the three peptides ...200818971945
network "rodent-borne pathogens" in germany: longitudinal studies on the geographical distribution and prevalence of hantavirus infections.hantavirus infections are known in germany since the 1980s. while the overall antibody prevalence against hantaviruses in the general human population was estimated to be about 1-2%, an average of 100-200 clinical cases are recorded annually. in the years 2005 and 2007 in particular, a large increase of the number of human hantavirus infections in germany was observed. the most affected regions were located in the federal states of baden-wuerttemberg, bavaria, north rhine westphalia, and lower s ...200819030894
emergence of hantavirus in south germany: rodents, climate and human infections.human hantavirus (serotype puumala) infections are prevalent throughout europe. the bank vole is the main reservoir of the puumala virus (puuv). between 2001 and 2006, the annual incidences in germany ranged from 0.1 to 0.5 per 100,000 inhabitants. about half of the cases were reported from the state of baden-württemberg (bw) in southwest germany. in 2007, 1,089 puuv infections were reported from bw. this reflects an incidence of 10.1:100,000, which is more than 11 times higher than the mean inc ...200819030895
[the reactivity of sera from hemorrhagic fever in patients with renal syndromes to the recombination nucleotide proteins from european hantaviruses in hubei province].five yeast-expressed recombination nucleotide proteins of european hantaviruses were prepared as coated antigens to detect hantavirus-specific antibodies in sera from hemorrhagic fever with renal syndromes (hfrs) in hubei province, through elisa assay. the relativity among hantaviruses prevailing in different areas was investigated.200819040041
antiviral effect of human saliva against hantavirus.hemorrhagic fever with renal syndrome (hfrs) and hantavirus pulmonary syndrome are zoonotic diseases caused by rodent borne hantaviruses. transmission to humans occurs usually by inhalation of aerozolized virus-contaminated rodent excreta. although human-to-human transmission of andes hantavirus has been observed, the mode of transmission is currently not known. saliva from puumala hantavirus (puuv)-infected patients was shown recently to contain viral rna. to test if human saliva interferes wit ...200819040288
imported case of nephropathia epidemica--a case report.a 41-year-oldpatient was hospitalized in hypotensive phase of nephropathia epidemica. the diagnosis was confirmed by serology. it is highly likely that the patient was exposed to infected aerosol of bank voles in endemic area of sweden. the disease clinically manifested with fever, headache, abdominal and back pain, vision disturbances. pathologic laboratory findings included thrombocytopenia, leukocytosis, increased values of c-reactive protein, blood urea and creatinine, abnormal chest x-ray a ...200819149238
[study on molecular epidemiology of puumala-like virus]. 200819173894
genetic and antigenic analyses of a puumala virus isolate as a potential vaccine strain.puumala virus (puuv), a causative agent of hemorrhagic fever with renal syndrome (hfrs), is prevalent in europe and european russia. no vaccine has been developed for puuv-associated hfrs, primarily because of the low viral yield in cultured cells. a puuv strain known as dtk/ufa-97 was isolated in russia and adapted for growth in vero e6 cells maintained in serum-free medium. the dtk/ufa-97 strain produced a higher viral titer in serum-free medium, suggesting that it may prove useful in the deve ...200819177744
hantaviruses: an emerging public health threat in india? a review.the emerging viral diseases haemorrhagic fever with renal syndrome (hfrs) and hantavirus cardiopulmonary syndrome (hcps) are a cause of global concern as they are increasingly reported from newer regions of the world. the hantavirus species causing hfrs include hantaan virus,seoul virus, puumala virus, and dobrava-belgrade virus while sin nombre virus was responsible for the 1993 outbreak of hcps in the four corners region of the us. humans are accidental hosts and get infected by aerosols gener ...200819208975
puumala hantavirus genetic variability in an endemic region (northern sweden).puumala hantavirus (puuv), naturally harboured and shed by bank voles (myodes [clethrionomys] glareolus), is the etiological agent to nephropathia epidemica (ne), a mild haemorrhagic fever with renal syndrome. both host and virus are found throughout much of the european continent and in northern sweden ne is the second most prevalent serious febrile viral infection after influenza. the reliability of diagnostics by pcr depends on genetic variability for the detection of viral nucleic acids in u ...200818296126
[increase in the number of cases of epidemic nephropathy in germany. virological and ecological aspects].epidemic nephropathy (en) is transmitted to humans via rodents. the causative agent of this virus-borne renal disease is the hantavirus puumala. other members of the genus hantavirus cause hemorrhagic fever with renal syndrome (hfrs) or hantaviral pulmonary syndrome (hps). as with all zoonoses the prevalence of the virus depends on the distribution of the reservoir species. climate changes have direct impact on the number of host animals and influence the incidence of hantaviral infections. a nu ...200818302100
hantavirus causing hemorrhagic fever with renal syndrome enters from the apical surface and requires decay-accelerating factor (daf/cd55).the old world hantaviruses, members of the family bunyaviridae, cause hemorrhagic fever with renal syndrome (hfrs). transmission to humans occurs via inhalation of aerosols contaminated with the excreta of infected rodents. the viral antigen is detectable in dendritic cells, macrophages, lymphocytes, and, most importantly, microvascular endothelial cells. however, the site and detailed mechanism of entry of hfrs-causing hantaviruses in polarized epithelial cells have not yet been defined. theref ...200818305044
spatial and temporal patterning of bank vole demography and the epidemiology of the puumala hantavirus in northeastern france.epidemiological data from bank voles, myodes glareolus, naturally infected by the hantavirus puumala (puuv) were collected by a capture-mark-recapture protocol from 2000 to 2002 in the french department of ardennes. four monitored trapping sites were established in two forests located in two cantons (flize and monthermé). we captured 912 bank voles corresponding to 557 different individuals during 8820 trapping nights for an overall trapping success of 10.34%. the average puuv seroprevalence was ...200818325126
hantavirus rna in saliva from patients with hemorrhagic fever with renal syndrome.hantaviruses cause 2 zoonotic diseases, hemorrhagic fever with renal syndrome (hfrs) and hantavirus cardiopulmonary syndrome. infection is usually initiated after inhalation of virus-contaminated rodent excreta. in addition to the zoonotic infection route, growing evidence suggests person-to-person transmission of andes virus. for this reason, we studied whether saliva from hfrs patients contained hantavirus. during an outbreak in northern sweden of nephropathia epidemica (ne), a milder form of ...200818325254
truncated recombinant puumala virus nucleocapsid proteins protect mice against challenge in europe, puumala virus and dobrava virus are the major hantaviruses that cause hemorrhagic fever with renal syndrome in humans. as hantaviruses can cause diseases with high morbidity and mortality rates, and as to date there is no specific treatment, efforts are concentrated on the development of vaccines. in this study we characterized the immunogenicity of recombinant nucleocapsid proteins of puumala virus (puuv) linked to a carrier protein corresponding to the outer membrane protein a from ...200818355122
population, environmental, and community effects on local bank vole (myodes glareolus) puumala virus infection in an area with low human this study, the distribution of puumala hantavirus (puuv) infection in local bank vole myodes glareolus populations in an area with low human puuv infection (nephropathia epidemica [ne]) incidence in northern belgium was monitored for 2 consecutive years. bank voles were trapped in preferred habitat and tested for anti-puuv igg. infection data were related to individual bank vole features, population demography, and environmental variables. rare occurrence of puuv infection was found and puuv ...200818370592
[discovery, clinical and etiological characteristic of hemorrhagic fever with renal syndrome in the subtropical zone of krasnodar region].twenty-six patients with hemorrhagic fever with renal syndrome (hfrs) were revealed as a result of serological examination of 582 patients with fever living around sochi town. etiologic role of dobrava virus subtype as the cause of hfrs was assessed by immunofluorescent and elisa assays, and neutralization test. the principal host of this virus and source of infection for humans is caucasian forest mouse apodemus ponticus. hfrs morbidity was sporadic and not dependent from patients' occupation a ...200818376466
passive immunization protects cynomolgus macaques against puumala hantavirus challenge.hantaviruses cause two severe and often fatal human diseases: haemorrhagic fever with renal syndrome (hfrs) and hantavirus pulmonary syndrome (hps). presently, there is no effective prevention available for hfrs or hps. here, we studied the effect of passive immunization on the course of infection in cynomolgus macaques challenged with wild-type puumala hantavirus (puuv-wt).200818389907
hemorrhagic fever with renal syndrome caused by 2 lineages of dobrava hantavirus, russia.dobrava-belgrade virus (dobv) is a european hantavirus that causes hemorrhagic fever with renal syndrome (hfrs); case-fatality rates in balkan countries are as high as 12%. to determine causative agents, we examined 126 cases of dobv-associated hfrs in central and southern european russia. in central russia (lipetsk, voronezh, orel regions), outbreaks were caused by a dobv variant (dobv-aa) carried by apodemus agrarius. in southern russia (sochi district), where hfrs is endemic, hfrs cases were ...200818394280
high rates of molecular evolution in hantaviruses.hantaviruses are rodent-borne bunyaviruses that infect the arvicolinae, murinae, and sigmodontinae subfamilies of muridae. the rate of molecular evolution in the hantaviruses has been previously estimated at approximately 10(-7) nucleotide substitutions per site, per year (substitutions/site/year), based on the assumption of codivergence and hence shared divergence times with their rodent hosts. if substantiated, this would make the hantaviruses among the slowest evolving of all rna viruses. how ...200818417484
outbreak of puumala virus infection, unexpected and large outbreak of puumala virus infection in sweden resulted in 313 nephropathia epidemica patients/100,000 persons in västerbotten county during 2007. an increase in the rodent population, milder weather, and less snow cover probably contributed to the outbreak.200818439368
hantavirus outbreak, germany, 2007. 200818439382
[detection of a new natural virus focus dobrava in the astrakhan region].a natural focus of dobrava hantavirus was first revealed in an arid zone of the astrakhan region, by using molecular genetic methods. a polymerase chain reaction was employed to examine 389 lung tissue suspension samples taken from 9 species. hantavirus rna was found in 35 samples from 22 field mice (apodemus agrarius), 8 tamarisk gerbils (meriones temariscinus), 4 common voles (microtus arvalis), and 1 house mouse (mus musculus). sequencing determined the taxonomic affiliation of 18 new isolate ...200818450108
mixing of m segment dna vaccines to hantaan virus and puumala virus reduces their immunogenicity in determine if dna vaccines for two hantaviruses causing hemorrhagic fever with renal syndrome, hantaan virus and puumala virus, are immunogenic when given in combination, we delivered them to hamsters separately or as mixtures by gene gun or by electroporation. both vaccines elicited neutralizing antibodies when given alone but when they were delivered as a mixture, antibodies to only one of the two hantaviruses could be detected. in contrast, if the dnas were given as separate vaccinations to ...200818482782
[hantavirus infections].hantaviruses are enveloped single-stranded ribonucleic acid (rna) viruses that belong to the family bunyaviridae. they are widely distributed and maintained in populations of specific insectivores or rodent host species (mice or rats). in their natural host, hantaviruses develop persistent and asymptomatic infections with lifelong virus shedding in excreta. transmission to humans occurs via direct contact with faeces, urine and saliva of infected rodents, in particular by inhaling virus contamin ...200818497244
[fever, nosebleeding and myalgic abdominal pain].a 38 year old patient presented with fever, myalgic abdominal pain, nose bleeding and acute renal failure since five days. a combination of thrombocytopenia, proteinuria, elevated crp and creatinin is common in hemorrhagic fever with renal syndrome (hfrs) due to hantavirus infection. the benigne form is called nephropathia epidemica. dialysis is infrequently required by patients with the puumala virus. other infection (e.g. malaria, leptospirosis, yellow fever) and systemic diseases (e.g. collag ...200818548800
accumulation of point mutations and reassortment of genomic rna segments are involved in the microevolution of puumala hantavirus in a bank vole (myodes glareolus) population.the genetic diversity of puumala hantavirus (puuv) was studied in a local population of its natural host, the bank vole (myodes glareolus). the trapping area (2.5 x 2.5 km) at konnevesi, central finland, included 14 trapping sites, at least 500 m apart; altogether, 147 voles were captured during may and october 2005. partial sequences of the s, m and l viral genome segments were recovered from 40 animals. seven, 12 and 17 variants were detected for the s, m and l sequences, respectively; these r ...200818559935
genetic analysis of hantaviruses carried by myodes and microtus rodents in buryatia.hantavirus genome sequences were recovered from tissue samples of myodes rufocanus, microtus fortis and microtus oeconomus captured in the baikal area of buryatia, russian federation. genetic analysis of s- and m-segment sequences of buryatian hantavirus strains showed that myodes-associated strains belong to hokkaido virus (hokv) type while microtus-associated strains belong to vladivostok virus (vlav) type. on phylogenetic trees buryatian hokv strains were clustered together with m. rufocanus- ...200818190679
case 31-2007: a man with abdominal pain and elevated creatinine. 200818199876
cross-reactive and serospecific epitopes of nucleocapsid proteins of three hantaviruses: prospects for new diagnostic tools.the diagnosis of infectious diseases is sometimes difficult because of extensive immunological cross-reactivity between related viral antigens. on the path of constructing sero-specific antigens, we have identified residues involved in sero-specific and cross-reactive recognition of the nucleocapsid proteins (nps) of puumala virus (puuv), seoul virus (seov), and sin nombre virus (snv) using serum samples from 17 nephropathia epidemica patients. the mapping was performed by enzyme-linked immunoso ...200818620010
hantavirus nephropathy.pathogenic rodent-borne hantaviruses cause in humans generalized infections that involve the peripheral vascular bed and severely affect their permeability. we describe a 30-yr-old male patient with clinical symptoms characterizing five conventional phases of hemorrhagic fever with renal syndrome after an uncommonly severe hantavirus infection with the puumala strain. renal biopsy in this situation typically demonstrates acute hemorrhagic interstitial nephritis, particularly pronounced in the ou ...200818650483
puumala hantavirus excretion kinetics in bank voles (myodes glareolus).puumala hantavirus is present in bank voles (myodes glareolus) and is believed to be spread mainly by contaminated excretions. in this study, we subcutaneously inoculated 10 bank voles with puumala virus and sampled excretions until day 133 postinfection. levels of shed viral rna peaked within 11-28, 14-21, and 11-28 days postinfection for saliva, urine, and feces, respectively. the latest detection of viral rna was 84, 44, and 44 days postinfection in saliva, urine, and feces, respectively. in ...200818680643
pathology of puumala hantavirus infection in macaques.hantaviruses are globally important human pathogens that cause hemorrhagic fever with renal syndrome and hantavirus pulmonary syndrome. capillary leakage is central to hantaviral diseases, but how it develops, has remained unknown. it has been hypothesized that the pathogenesis of hantavirus infection would be a complex interplay between direct viral effects and immunopathological mechanisms. both of these were studied in the so far best model of mild hemorrhagic fever with renal syndrome, i.e. ...200818716663
serological evidence of viruses naturally associated with the montane water vole (arvicola scherman) in eastern france.we surveyed 12 populations of the montane water vole (arvicola scherman), previously known as the fossorial form of the water vole a. terrestris, in eastern france for antibodies (immunoglobulin g) to puumala virus (puuv), lymphocytic choriomeningitis virus (lcmv), and cowpox virus (cpxv). antibodies to puuv were found in 9 (5.5%) of 164 voles from 7 populations, antibodies to lcmv were found in 13 (26.0%) of 50 voles from 2 populations, and antibodies to cpxv were found in 66 (41.8%) of 158 vol ...200818752422
[immunogenicity of n-protein of puumala hantavirus for noninbred mice in the intramuscular administration of its gene dna].the outbreaks of hantavirus infections in some regions of the russian federation in some years involve considerable material and social losses. in this connection, the designing of the most effective types of vaccines is an urgent task. the authors have created plasmid constructions containing the gene of hemorrhagic fever with renal syndrome virus, with whose intramuscular injection there is a specific immune response and plasmid dna is detectable in the adjacent tissues within a month after in ...200818756816
[nephropathia epidemica as the result of a puumala virus infection in a pregnant patient].a 29-year-old woman in the early stage of pregnancy ( approximately 6 (th) week) presented in our hospital with influenza-like symptoms of fever, headache, aching limbs and tenderness over the costovertebral angle. acute oliguric renal failure occurred within a few days.200818770481
more than half of the patients with acute puumala hantavirus infection have abnormal cardiac findings.this study was conducted to determine the frequency, severity and outcome of cardiac findings in patients with acute puumala hantavirus-induced nephropathia epidemica (ne). 70 consecutive, hospital-treated patients with serologically confirmed ne were prospectively examined using serial electrocardiograms (ecg), plasma troponin i, tumour necrosis factor alpha (tnf-alpha), interleukin-6 (il-6), and echocardiography (echo). examinations were repeated after 3 and 12 months. ecg changes were observe ...200918932105
hantavirus infections in europe: from virus carriers to a major public-health europe, hantavirus disease or hemorrhagic fever with renal syndrome is an endemic zoonosis that affects tens of thousands of individuals each year. the causative agents are viruses of the genus hantavirus, family bunyaviridae, rodents and insectivores act as carriers. in all european countries there is a seroprevalence for hantaviruses in the general population but not all countries report cases. here, we give an overview of the hantavirus situation in europe.200919254169
association between habitat and prevalence of hantavirus infections in bank voles (myodes glareolus) and wood mice (apodemus sylvaticus).in order to determine the habitat preferred by myodes (before clethrionomys) glareolus and the corresponding puumala hantavirus seroprevalence in those habitats, we captured rodents simultaneously in three significantly different habitats. we compared trapping success and presence of virus per habitat during an ongoing epidemic in order to test the hypothesis of a density-dependent seroprevalence. our study showed that bank vole population density, as well as puumala virus seroprevalence, were h ...200919271997
survey and case-control study during epidemics of puumala virus baden-wuerttemberg, a federal state in south-west germany, a large outbreak of 1089 laboratory-confirmed human puumala virus (puuv) infections occurred in 2007. we conducted a survey to describe the disease burden and a case-control study to identify risk factors for acquiring puuv. case-patients were interviewed about clinical outcome and both case-patients and randomly recruited controls were interviewed about exposure. we calculated matched odds ratios (mor) using a conditional logistic re ...200919288958
beware fat bank voles. 200919324293
analysis of puumala hantavirus in a bank vole population in northern finland: evidence for co-circulation of two genetic lineages and frequent reassortment between this study, for the first time, two distinct genetic lineages of puumala virus (puuv) were found within a small sampling area and within a single host genetic lineage (ural mtdna) at pallasjärvi, northern finland. lung tissue samples of 171 bank voles (myodes glareolus) trapped in september 1998 were screened for the presence of puuv nucleocapsid antigen and 25 were found to be positive. partial sequences of the puuv small (s), medium (m) and large (l) genome segments were recovered from thes ...200919386780
tubular proteinuria and glomerular filtration 6 years after puumala hantavirus-induced acute interstitial nephritis.we previously found increased urinary protein excretion, glomerular filtration rate (gfr) and blood pressure in a retrospective analysis of patients with previous nephropathia epidemica (ne). here, we evaluated the long-term outcome after ne in a prospectively recruited patient group.200919390211
the hantaviral load in tissues of naturally infected rodents.hantaviruses cause a lifelong and asymptomatic infection in naturally infected hosts as well as in experimentally infected rodents. understanding the ecology and pathogenesis of hantaviruses requires an interdisciplinary research approach, which links laboratory experiments with results gained from field studies. although several studies report hantavirus persistence and tissue infection patterns for experimentally infected rodents, field data is very limited. for this reason, the aim of our stu ...200919397875
serological microarray for detection of hsv-1, hsv-2, vzv, and cmv antibodies.the seroprevalence of human herpesviruses is high and reactivations occur frequently. a microarray was designed and tested for the detection of igg and igm antibodies for puumala hantavirus (puuv) and igg antibodies against four herpesviruses. initially, a microarray platform was set up using an unrelated in-house antigen, puuv recombinant nucleocapsid protein, to optimize the protocol for the detection of antibodies. detection of the four herpesviruses was set up in a microarray using the recom ...200919477202
nephropathia epidemica with a 6-week incubation period after occupational exposure to puumala hantavirus.serological and molecular evidence showed that a german female student became infected by puumala hantavirus during mice trapping efforts in finland. the incubation period before exhibiting clinical signs of nephropathia epidemica was as long as 6 weeks. phylogenetic analysis of puuv nucleic acid sequences amplified from the patient demonstrate that she was infected by the puuv strain circulating at the place of her occupational exposure in finland.200919058998
predicting high risk for human hantavirus infections, increased risk for hemorrhagic fever with renal syndrome caused by puumala hantavirus was forecast for sweden in 2007. the forecast was based on a predicted increase in the number of myodes glareolus rodents (reservoir hosts). despite raised awareness and preparedness, the number of human cases during july 2007-june 2008 was 1,483, a new high.200919116065
seroepidemiological study in a puumala virus outbreak area in south-east germany.puumala virus (puuv) is the cause of the majority of haemorrhagic fever with renal syndrome cases in germany. in 2004, a nephropathia epidemica outbreak was recorded in lower bavaria, south-east germany. for a seroepidemiological study in this region including the resident population at four locations (n = 178) and soldiers from one location (n = 208) indirect immunoglobulin m (igm) and immunoglobulin g (igg) enzyme-linked immunosorbent assays (elisas) and immunoblot tests based on a yeast-expre ...200919148676
hantavirus disease (nephropathia epidemica) in belgium: effects of tree seed production and climate.recently, human cases of nephropathia epidemica (ne) due to puumala virus infection in europe have increased. following the hypothesis that high reservoir host abundance induces higher transmission rates to humans, explanations for this altered epidemiology must be sought in factors that cause bank vole (myodes glareolus) abundance peaks. in western europe, these abundance peaks are often related to high tree seed production, which is supposedly triggered by specific weather conditions. we evalu ...200918606026
[cloning and expression of the nucleoprotein gene of puumala-like virus].in order to detect hokkaido virus (hokv), a recombinant baculovirus containing the nucleoprotein (np) gene of hokv was constructed, and then the np was expressed in insect cell.200919565881
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