TitleAbstractYear(sorted ascending)
[viral infections--clinical pictures and laboratory diagnosis. part 3: hantaan virus and hantaan-related viruses, hepatitis a and b]. 19937905848
acute hantavirus infection. 19937905899
[von willebrand factor, antithrombin iii and 5'-nucleotidase in the blood and the athrombogenic properties of the vascular wall in patients with hemorrhagic fever with renal syndrome].willebrand's factor (wf), antithrombin iii (at-iii), blood 5'-nucleotidase and athrombogenic properties of the vascular wall were studied in the course of the disease in 69 patients suffering from hemorrhagic fever with renal syndrome (hfrs). the patients were chiefly under 27 years, had severe or moderate condition. it was found that wf rose 3-fold in oliguria, but returned to normal values by convalescence. at-iii remained stable. high activity of 5'-nucleotidase reached the pick in oliguria a ...19937906437
application of a recombinant baculovirus expressing hantavirus nucleocapsid protein as a diagnostic antigen in ifa test: cross reactivities among 3 serotypes of hantavirus which causes hemorrhagic fever with renal syndrome (hfrs).recombinant baculovirus-infected insect cells which expressed recombinant protein, analogous to the nucleocapsid protein (np) of seoul type hantavirus, strain sr-11 (rnp-sr-sf9) were applied to the indirect immunofluorescent antibody (ifa) test. the rnp-sr-sf9 reacted with anti np mab clones which recognized strain specific or hantavirus common epitopes in the ifa test. the recombinant antigen was detected by antibodies to 3 serotypes of hantaviruses (hantaan 76-118, sr-11, puumala). antibody ti ...19937906956
[atypical pneumonia during hemorrhagic fever with renal syndrome]. 19937907181
hantavirus: an old bug learns new tricks.hantaviruses are a diverse group of rna arboviruses in the bunyaviridae family. although their role as the causative agents of hfrs has been well established, the recent outbreak of a new disease in the southwest clearly demonstrates the protein clinical manifestations that this pathogen can produce. furthermore, whereas hantaviruses have been characterized largely as focal agents in the production of geographically delimited diseases, recent trends indicate that endemic areas for the virus are ...19937907616
hantavirus-associated illness identified in north dakota. 19937907617
second international conference on hemorrhagic fever with renal syndrome. 19937908259
raised plasma endothelin-1 concentration in patients with nephropathia epidemica.nephropathia epidemica (ne) with renal syndrome, caused by the puumala-virus, is manifested clinically by the triad of fever, hemorrhage and renal failure. we observed raised plasma concentrations of endothelin-1 (et-1) and atrial natriuretic peptide (anp) in 23 patients during the acute phase of ne. they all developed transient renal failure and all displayed characteristics of ne, also verified by a rapid igg antibody test. blood pressure was normal or low in all subjects during the acute phas ...19937900944
[hantavirus infections 1993: endemic or unrecognized pandemic?]. 19937900981
hantavirus disease emerging. 19937901592
comparison of virulence between seoul virus strain sr-11 and hantaan virus strain 76-118 of hantaviruses in newborn mice.virulence of hantavirus strain of sr-11 seoul virus and hantaan 76-118 (htn) of hantaan virus were compared. infections of both strains were lethal in newborn mice. however, inoculum required to cause lethal infection was about 4,000 times higher for strain htn (1.65 x 10(3) pfu/mouse/ld50) than for strain sr-11 (0.36 pfu). thus, both strains were considered pathogenic to newborn mice but they possessed different levels of virulence. the assay system used for these strains in newborn mice proved ...19937901740
a rogues' gallery of hantaviruses. 19937901910
update: hantavirus disease--united states, 1993. 19937902158
identifying a hantavirus associated with acute respiratory illness: a pcr victory? 19937902477
[report on detection of rats and humans with hfrs antibody in the faculty of medicine, kyoto university and on measures taken against the trouble]. 19937902816
[distribution of extracellular viral antigen of epidemic hemorrhagic fever in human autopsy cases].using improved immunohistochemical methods, repeated pap and repeated pap + abc, we found in the extracellular spaces of autopsy cases of epidemic hemorrhagic fever large amount of viral antigens. two forms of antigens were found: diffuse soluble antigen and inclusion body like granular antigen. the former was mainly g2 protein and the latter was mainly n protein. they were found in serum, lymph, csf, edema fluid, fluids in renal, seminal tubules, alveolar and bronchial spaces, peptic, intestina ...19937903062
p53 sweeps through cancer research. 19937903477
epizootiological study of hantavirus infection among rattus norvegicus in tokyo bay area, japan.the prevalence of antibodies against hantavirus in 413 rats (rattus norvegicus) captured in eight regions of the tokyo bay area from 1983 to 1992 were examined by the indirect fluorescent antibody test. antibody-positive rats were found in tokyo port in 1983 (34.8%), 1984 (25.9%), 1985 (22.0%) and 1986 (15.6%), in kasai seaside park in 1989 (3.2%) and 1990 (4.2%) and in chiba port in 1990 (6.7%). in tokyo port, antibody-positive rats were found in any season in 1984 and 1985, and seroprevalences ...19937903711
[hemorrhagic fever with renal syndrome in the structure of natural-focus morbidity in the southern khabarovsk territory and its social significance].according to the data contained in the materials provided by the hospital of infectious diseases of the khabarovsk medical institute, in the recent decade hemorrhagic fever with the renal syndrome, together with pseudotuberculosis, played the leading role in the structure of morbidity caused by natural foci of infections. the distribution of patients in social groups and different important factors of the epidemiology of this disease are considered. taking into account a high morbidity level in ...19937915073
hantavirus infection in brazilian patients from recife with suspected leptospirosis. 19938093292
infections in an alpine environment: antibodies to hantaviruses, leptospira, rickettsiae, and borrelia burgdorferi in defined italian populations.from 1987 to 1991, a seroepidemiologic survey for antibodies to hantaviruses, leptospira, rickettsiae, and borrelia was conducted in selected italian population groups. in the mountainous areas of northeastern italy, the prevalence of antibody to hantaviruses, as detected by indirect immunofluorescent antibody (ifa) assay, was 7.1%, 4.8%, 4.3%, and 4% in 265 forestry workers, 82 rangers, 395 farmers, and 75 hunters, respectively. among 299 alpine soldiers, the prevalence was lower (0.7%). of tho ...19938093990
immunoglobulin g subclass responses against the structural components of puumala virus.sera from sequentially bled nephropathia epidemica patients (acute-phase, convalescent-phase, and 2-year sera) and sera from 10 to 20 years postinfection were examined by immunoglobulin g (igg) subclass-specific elisas for reactivities against each of the puumala virus structural proteins (n, g1, and g2). igg1 was found to be the dominating antiviral subclass, and most of the patients had igg1 directed to all three structural proteins at all times with a continuous increase over time. igg2 to th ...19938094397
infection with a ratborne hantavirus in us residents is consistently associated with hypertensive renal disease.a survey of 8080 subjects was conducted in baltimore, examining the association between infection with hantaviruses and renal disease. two groups (n = 6060) with no known risk factors were selected to establish a baseline antibody prevalence. overall, antibody prevalence was 0.25%. seroprevalence increased with age, without sex- or race-related differences. patients with proteinuria showed the same patterns of infection but were more commonly seropositive (1.46%) than the reference group (or, 3. ...19938095060
metabolism of calcium and vitamin d3 in patients with acute tubulointerstitial nephritis: a study of 41 patients with nephropathia epidemica.we studied serum concentrations of calcium, phosphate, intact parathyroid hormone (pth) and vitamin d3 metabolites in 41 patients with nephropathia epidemica. thirty-four of the 41 patients had a mild to moderate, mostly nonoliguric acute renal failure (arf). hypocalcemia developed in relation to the severity of renal failure, and parathyroid gland response to hypocalcemia was normal. the serum concentration of 1,25-dihydroxyvitamin d3 was lower than normal in patients who developed arf. serum p ...19938095698
acute hantavirus infection presenting as hypersensitivity vasculitis with arthropathy.acute hantavirus infection was diagnosed in a young man presenting with a hypersensitivity vasculitis and arthropathy. he has recovered with minimal evidence of residual renal damage.19938095959
puumala virus antibody and immunoglobulin g avidity assays based on a recombinant nucleocapsid antigen.puumala virus is the causative agent of nephropathia epidemica (ne), a hantavirus infection which occurs widely in northern and central europe and is generally diagnosed by the indirect immunofluorescence (if) method. we have now expressed the puumala virus sotkamo strain nucleocapsid (n) protein-coding s genome segment as a beta-galactosidase fusion protein in escherichia coli by using the pex2 expression vector. the recombinant protein was purified by cutting the protein band from an agarose g ...19938096217
haemorrhagic fever with renal syndrome and pregnancy: a case report. 19938096661
use of recombinant nucleocapsid proteins of the hantaan and nephropathia epidemica serotypes of hantaviruses as immunodiagnostic antigens.hantavirus nucleocapsid protein has previously been identified as the major antigen recognized by the humoral immune response in hemorrhagic fever with renal syndrome (hfrs). it was therefore considered to be a suitable antigen for the development of rapid and reliable immunodiagnostic assays. genes encoding the nucleocapsid proteins of two hantavirus strains, one of the puumala serotype [nephropathia epidemica virus (nev)] and the other of the hantaan serotype were expressed in e. coli, and the ...19938096868
lack of evidence of puumala virus infection in patients with spontaneous abortion. 19938099010
a novel mu-capture enzyme-linked immunosorbent assay based on recombinant proteins for sensitive and specific diagnosis of hemorrhagic fever with renal syndrome.hantavirus nucleocapsid protein has recently been identified as a major antigen inducing an early and long-lasting humoral immune response in patients with hemorrhagic fever with renal syndrome. a mu-capture enzyme-linked immunosorbent assay utilizing recombinant nucleocapsid proteins of hantavirus strains hantaan 76-118 (hantaan serotype) and cg 18-20 (puumala serotype) as diagnostic antigens and specific monoclonal antibodies as the detection system has been developed. histidine-tailed recombi ...19938099085
the humoral response to puumala virus infection (nephropathia epidemica) investigated by viral protein specific immunoassays.enzyme-linked immunosorbent assays for determination of antibodies directed to the nucleocapsid protein (n) or to either of the two envelope glycoproteins (g1 and g2) of puumala virus were designed and evaluated. the assays were proven to be entirely restricted for each viral structural protein by biotin-labelled monoclonal antibodies. sera from sequentially bled nephropathia epidemica patients (acute, convalescent, and 2-year sera) and sera from 10-20 year convalescents were examined for antibo ...19938099274
[the cohort study on inapparent infection of hfrs in population of apodemus-type endemic area and in that of rattus-type endemic area].between 1988 and 1990, the positive seroconversion rates of hfrs were tested with a random sample of 7,145 persons in apodemus-type endemic area and rattus-type endemic area(type a; type r) in shandong province. the results showed that: (1) the highest positive seroconversion rate was in the group of peasants of 20 to 49 years of age in the area of type a; (2) in the area of type r, there was no statistically significant difference in positive seroconversion rates of age, and housewives had the ...19938099308
[serotyping of patients with epidemic hemorrhagic fever (ehf) and serotyping and antigen typing of main hosts of ehf in shandong province].serotyping with hemagglutination-inhibition test (hi) on 458 patients with ehf showed that 419 (91.48%) of them belonged to the house-rat type, 33 (7.21%) belonged to the wild-rat type, and 6 (1.31%) were undetermined. the majority (80-100%) of the patients in various regions belonged to the house-rat type. the type of the virus antigen and the type of the antibody of rats were in conformity with the species of rats. these indicate that the epidemic areas of ehf in shandong province are mixed-ty ...19938099309
usa: mysterious respiratory illness. 19938099392
expression of non-conserved regions of the s genome segments of three hantaviruses: evaluation of the expressed polypeptides for diagnosis of haemorrhagic fever with renal syndrome.haemorrhagic fever with renal syndrome (hfrs) is a serious and often fatal disease caused by viruses in the hantavirus genus of the family bunyaviridae. we expressed the entire coding region of the small (s) genome segments of three serologically distinct hantaviruses as soluble proteins in escherichia coli and evaluated the expressed nucleocapsid proteins (nps) as antigens for diagnosis of hfrs. we also prepared novel diagnostic antigens by expressing truncated genes from which we deleted amino ...19938099603
an epidemiologic study of hemorrhagic fever with renal syndrome in bashkirtostan (russia) and sweden.the incidence and antibody prevalence of hemorrhagic fever with renal syndrome (hfrs) in bashkirtostan (european part of russia) and northern sweden was compared with the abundance of clethrionomys glareolus (bank voles) in the two areas. in bashkirtostan, 10% of the women and 15% of the men were found to be antibody positive. the corresponding figures for sweden were 8% and 16% for women and men, respectively. the annual incidence of hfrs in bashkirtostan was 50 cases per 100,000 inhabitants, w ...19938100119
mysterious respiratory disease in usa. 19938100340
[structural protein of hemorrhagic fever renal syndrome virus in uric fusion cells in hemorrhagic fever renal syndrome].the efoliative cells in the urine of 21 hemorrhagic fever patients with renal syndrome (hfrs) were examined with swiss blue staining. fusion cells were found in the urine of 18 hfrs patients. the quantity of fusion cells was closely related to the severity of patients' condition and renal damage. specific antigen of hfrs virus (hfrsv) was found immunohistochemically in all fusion cells. specific monoclonal antibodies (mcab) of membrane protein and nucleocapsid protein from hfrsv were used for an ...19938100735
emerging infectious diseases. outbreak of acute illness. 19938101098
community-based prevalence profile of arboviral, rickettsial, and hantaan-like viral antibody in the nile river delta of determine the current prevalence of antibody to arboviruses, rickettsiae, and hantaan-like viruses, a survey was carried out in the nile river valley of egypt, one of the principal foci of the 1977-1978 rift valley fever (rvf) outbreak. blood specimens were obtained from 915 persons representing 190 study households. enzyme immunoassay testing showed that the overall prevalence of igg antibody was 4% to sand fly fever sicilian (sfs), 2% to sandfly fever naples (sfn), 15% to rvf, 20% to west n ...19938101432
haemorrhagic fever with renal syndrome. 19938101451
presence of antibodies to hantavirus in rat and human populations of djibouti. 19938101667
molecular and antigenic characterization of hv114, a hantavirus isolated from a patient with haemorrhagic fever with renal syndrome in china.the relationship of a hantaan-like virus (hv114), isolated from a patient with haemorrhagic fever with renal syndrome in hubei province, people's republic of china, to other pathogenic hantaviruses was evaluated by cross-neutralization studies and nucleotide sequence analysis of the m genome segment. plaque reduction neutralization assays indicated that hv114 is closely related to prototype hantaan (htn) virus, strain 76-118, which was originally isolated from an apodemus field mouse in korea. c ...19938102176
outbreak of acute illness--southwestern united states, 1993. 19938102294
the clinical spectrum of human hantavirus infection in somerset, uk.the signs and symptoms exhibited by 29 patients with an acute illness in whom antibodies to hantavirus were detected are described. in the severe cases the most striking signs and symptoms were the persistence for 2 or 3 weeks of a severe sore throat with pyrexia that developed early in the illness, followed by swelling of the face, neck and extremities, with arthropathy and prolonged malaise that lasted for months. a macular erythematous rash, hepatomegaly with abnormal liver function tests, an ...19938102335
belgrade virus, a cause of hemorrhagic fever with renal syndrome in the balkans, is closely related to dobrava virus of field mice.belgrade virus is a recently described hantavirus that causes severe hemorrhagic fever with renal syndrome (hfrs) in people living in various parts of the balkan peninsula. nucleotide sequencing of the g2-encoding region in the medium (m) segment of the viral genome, reverse transcribed and amplified by the polymerase chain reaction, revealed the belgrade virus to be substantially different from hantaan virus and other major serotypes of hantavirus but identical to dobrava virus, a virus isolate ...19938102632
[vole fever. a european type of hemorrhagic fever with renal syndrome]. 19938104262
igg avidity assay for estimation of the time after onset of hantavirus infection in colonized and wild bank immunoglobulin g avidity assay was used to determine recent and past hantavirus infection in bank voles (clethrionomys glareolus). sera of experimentally infected bank voles were studied at different time intervals. the avidity of specific igg increased over time after infection. this experimental data were used to estimate the time after onset of hantavirus infection in naturally infected bank voles caught in an endemic area. the possibility to discriminate between recently infected animals ...19938104397
liver involvement in hemorrhagic fever with renal syndrome.hemorrhagic fever with renal syndrome is an acute febrile nephropathy caused by several viruses of the genus hantavirus of the family bunyaviridae. during the past 8 years, 32 cases of hemorrhagic fever with renal syndrome have been diagnosed. nine out of the 32 patients (28%) had raised (twofold upper normal limits) transaminases during hospitalization. four out of the nine patients (44%) died. in contrast, only three out of the 23 patients (13%) without raised transaminases died. five patients ...19938104972
genetically distinct hantavirus in deer mice. 19938105294
[alternanthera philoxeroides (mavt.) griseb protection against fetal epidemic hemorrhagic fever virus infection in suckling mice].it has been confirmed that alternanthera philoxeroides (apg) can markedly protect suckling mice from being infected by epidemic hemorrhagic fever (ehf) virus. after the infected mice were treated with the effective components their survival rate increased, pathological lesion and virus antigen in the tissues mitigated as compared with the controls. therapeutical dose of apg caused only slight deformation of the hepatic cells.19938105801
[a combined staining of immunohistochemistry and in situ hybridization for detecting epidemic hemorrhagic fever virus in tissue section].a combined staining of immunohistochemistry and in situ hybridization has been successfully applied to detect antigen and viral rna at the same tissue slice from epidemic hemorrhagic fever (ehf) autopsy. multiple mcab were used to detect ehfv antigen, followed with a digoxigenin-labelling cdna probe for detecting ehfv rna respectively. the viral antigen was seen in the cellular cytoplasm and viral rna was demonstrated within the nuclei. it is emphasized that pretreatment of the section with lugo ...19938106176
utilization of autopsy rna for the synthesis of the nucleocapsid antigen of a newly recognized virus associated with hantavirus pulmonary syndrome.a newly recognized hantavirus was recently found to be associated with an outbreak of acute respiratory illness in the southwestern united states. the disease, which has become known as hantavirus pulmonary syndrome, has an unusually high mortality (64%). virus isolation attempts have been unsuccessful thus far, resulting in a lack of homologous antigen for use in diagnostic assays. for this reason, a molecular approach was initiated to produce recombinant homologous antigen. the virus nucleocap ...19938109165
[the level of ionized calcium in the blood and parathyroid function in acute kidney failure in patients with hemorrhagic fever with renal syndrome].in patients with haemorrhagic fever with renal syndrome (hfrs) and in those who had hfrs in the past ionized calcium (ca2+) and parathyroid hormone (pth) levels were measured in the blood. high levels of pth in normal or low those of ca2+ were typical for hfrs oliguria. hyperparathyroidism observed was assessed as an adaptation reaction preventing oliguria-related ca2+ fall. in cured patients neither ca2+ nor pth deviation were reported.19937909180
recognition of hantavirus infection in the rural setting: report of first colorado resident to survive.since may 1993, there has been an outbreak of an acute illness with nonspecific symptoms and signs that rapidly progresses to death. the majority of the cases identified have occurred in the rural southwestern united states, predominantly in the four-corners area comprising new mexico, arizona, utah, and colorado. findings of preliminary investigation by the centers for disease control and prevention suggest that the etiologic agent is a previously unidentified strain of hantavirus. in reported ...19938117354
from the centers for disease control and prevention. update: hantavirus pulmonary syndrome--united states, 1993. 19938230584
hantavirus-associated acute respiratory failure. 19938232482
were four corners victims biowar casualties? 19938235553
hantavirus outbreak yields to pcr. 19938235602
the mouse-piñon nut connection. 19938235603
hantavirus pulmonary syndrome: an emerging infectious disease. 19938235607
genetic identification of a hantavirus associated with an outbreak of acute respiratory illness.a mysterious respiratory illness with high mortality was recently reported in the southwestern united states. serologic studies implicated the hantaviruses, rodent-borne rna viruses usually associated elsewhere in the world with hemorrhagic fever with renal syndrome. a genetic detection assay amplified hantavirus-specific dna fragments from rna extracted from the tissues of patients and deer mice (peromyscus maniculatus) caught at or near patient residences. nucleotide sequence analysis revealed ...19938235615
puumala virus infection without signs of renal involvement.five cases of puumala virus infection without renal involvement are described. the main signs were fever, headache, chills and thirst. pulmonary involvement was also common. neither increased levels of serum creatinine nor abnormal urinalyses were detected. we suggest that hantavirus infection is included in the differential diagnosis of any febrile condition when exposure to aerosols from infectious secretions or secretions from rodents is suspected.19938248755
race to grow hantavirus ends in tie. 19938248797
comparison of nucleotide sequences among hantaviruses belonging to the same serotype: an analysis of amplified dna by thermal cycle sequencing.the hantavirus genus, belonging to the bunyaviridae family, is comprised of at least four serologically distinct types: hantaan, seoul, puumala and prospect hill. previously, we reported the use of the polymerase chain reaction (pcr) for grouping hantavirus isolates by using four sets of primers specific to each serotype. our pcr typing results agreed with those of serological typing. the present study makes use of thermal cycle sequencing to sequence pcr-amplified dna products in order to deter ...19938249444
molecular approaches for the treatment of hemorrhagic fever virus infections.viruses causing hemorrhagic fevers in man belong to the following virus groups: togavirus (chikungunya), flavivirus (dengue, yellow fever, kyasanur forest disease, omsk hemorrhagic fever), arenavirus (argentinian hemorrhagic fever, bolivian hemorrhagic fever, lassa fever), filovirus (ebola, marburg), phlebovirus (rift valley fever), nairovirus (crimian-congo hemorrhagic fever) and hantavirus (hemorrhagic fever with renal syndrome, nephropathic epidemia). hemorrhagic fever virus infections can be ...19938250543
in utero and mammary transfer of hantavirus antibody from dams to infant rats.the effects of maternal-infant transfer of specific antibody on infection attributable to hemorrhagic fever with renal syndrome-causing virus in the infant rat were studied. in cross-fostering experiments, maternal hantavirus-specific antibody was shown to be transferred equally effectively to infants either in utero or by breastfeeding. both igg- and iga-specific antibodies were transferred. infected infants that initially acquired high levels of maternal antibodies by either route survived let ...19937908999
inability of a strain of seoul virus to transmit itself vertically in strain of seoul virus was inoculated into new-born female rats. these rats at the age of 13 weeks were mated with uninfected male rats. the virus was transmitted to the male rats, but the offsprings were not infected from their mather rat owing to the maternal antibody. no intrauterine transmission of the virus was observed. we assumed that sr-11 type strain of seoul virus could not be disseminated among rats in nature.19937914245
nucleotide and deduced amino acid sequences of the m and s genome segments of two puumala virus isolates from russia.hemorrhagic fever with renal syndrome (hfrs) is caused by viruses in the hantavirus genus, family bunyaviridae. three serologically distinct hantaviruses, hantaan, seoul and puumala viruses, are known to cause hfrs. we report here, for the first time, gene sequences of two human puumala virus isolates, p360 and k27, obtained in an hfrs endemic region of the former soviet union. we compared the nucleotide sequences and the derived amino acid sequences of their gene products to a puumala virus iso ...19938266723
research defines the hantavirus pulmonary syndrome. 19938307802
hantavirus in the southwestern united states: epidemiology of an emerging may of this year, an outbreak of sudden, unexplained, and highly fatal respiratory illness of unknown etiology was identified in the four-corners region of the southwestern united states. within 3 weeks after the collaborative response launched by the state and local health agencies in the affected area, laboratory studies from the federal centers for disease control and prevention suggested that an acute infection with a previously unrecognized hantavirus species was responsible for the repo ...19938307808
hantavirus pulmonary syndrome (hps): report of first case in louisiana.since may 1993, 26 people have died of a "unique, previously unknown" viral illness that presents with flu-like symptoms and progresses rapidly to respiratory failure and death. originally isolated in the four-corners region of the southwest, the hantavirus responsible has now been isolated from patients in several other areas. in this article, we describe our experience with "the most puzzling case so far," an infection by a "cousin" of the southwest hantaviral strain in a louisiana bridgeworke ...19938307809
from the centers for disease control and prevention. update: outbreak of hantavirus infection--southwestern united states, 1993. 19938315765
from the centers for disease control and prevention. update: hantavirus infection--united states, 1993. 19938320773
update: hantavirus disease--southwestern united states, 1993.since may 1993, the state health departments in arizona, colorado, new mexico, and utah; the indian health service; and cdc, with the assistance of the navajo nation division of health, have been investigating an outbreak of acute respiratory illness related to a newly recognized hantavirus (1-5). this report updates the investigation and presents information on a recent case of hantavirus-associated respiratory disease in a resident of nevada.19938332114
update: hantavirus disease--united states, 1993.since the recognition of acute hantavirus-associated respiratory disease in the united states in may 1993, laboratory evidence of acute hantavirus infection has been confirmed in 30 persons in the southwestern united states; 20 (67%) of these persons have died. of those 30 persons, 23 resided in the four-corners region (14 in new mexico, six in arizona, and three in colorado). previously reported cases outside the four-corners states occurred in a nevada resident and a texas resident, neither of ...19938336693
from the centers for disease control and prevention. update: hantavirus disease--southwestern united states, 1993. 19938345637
from the centers for disease control and prevention. update: hantavirus disease--united states, 1993. 19938355371
update: hantavirus-associated illness--north dakota, 1993. 19938361465
anti-idiotypic antibodies against puumala virus glycoprotein-specific monoclonal antibodies inhibit virus infection in cell cultures.rabbit anti-idiotypic antibodies (anti-ids) were generated against three bank vole and one human monoclonal antibody (mab) specific for the two envelope glycoproteins of puumala virus (g1 and g2). the anti-ids were purified by sequential immunoaffinity chromatography. each anti-id inhibited the antigen binding of its respective mab in a competitive elisa. this inhibition, and the absence of cross-reactivity among the anti-ids for heterologous mabs, showed that they all were specific for unique d ...19938379850
[study of cytopathogenic effect of epidemic hemorrhagic fever virus on normal human bone marrow cells].the cytopathogenic effect of epidemic hemorrhagic fever virus (ehfv) on cultured human bone marrow cells (hbc) was studied. specific viral antigen in hbc was detected at various times after ehfv infection with trypan-blue stain and electron microscopy. it was shown that the infection rate of hbc was over 80% at 3 weeks after infection. the death rate of hbc in the infection group was significantly higher than that in the control group on 1-4 weeks after infection. injury of cell membrane organs ...19938404319
non-viral sequences at the 5' ends of dugbe nairovirus s mrnas.analyses of the mrna transcription processes of viruses in four genera (bunyavirus, hantavirus, phlebovirus and tospovirus) of the family bunyaviridae have revealed a common mechanism of initiation using host-derived primers, known as cap-snatching. to provide similar information on the fifth genus in the family, the 5' ends of dugbe nairovirus s mrna species were specifically cloned and sequenced. this revealed the presence of non-viral heterogeneous sequences, five to 16 nucleotides in length ...19938409954
from the centers for disease control and prevention. update: hantavirus-associated illness--north dakota, 1993. 19938411540
from the centers for disease control and prevention. progress in the development of hantavirus diagnostic assays--united states. 19938411541
hantavirus infection--southwestern united states: interim recommendations for risk reduction. centers for disease control and prevention.this report provides interim recommendations for prevention and control of hantavirus infections associated with rodents in the southwestern united states. it is based on principles of rodent and infection control and contains specific recommendations for reducing rodent shelter and food sources in and around the home, recommendations for eliminating rodents inside the home and preventing them from entering the home, precautions for preventing hantavirus infection while rodent-contaminated areas ...19938413156
progress in the development of hantavirus diagnostic assays--united states. 19938413161
update: hantavirus pulmonary syndrome--united states, 1993.a unique hantavirus has been identified as the cause of the outbreak of respiratory illness (hantavirus pulmonary syndrome [hps]) first recognized in the southwestern united states in may 1993. the habitat of the principal rodent reservoir for this virus, peromyscus maniculatus (deer mouse), extends throughout most of the united states except the southeast. through october 21, 1993, hps has been confirmed in 42 persons reported to cdc from 12 states (figure 1). this report summarizes major clini ...19938413170
the effects of hybridoma growth factor in conditioned media upon the growth, cloning, and antibody production of heterohybridoma cell lines.interleukin 6 (il-6)/hybridoma growth factor (hgf) has been shown to be the requirement for growth of murine hybridomas in vivo or in vitro. in this paper, two kinds of conditioned media (cm) from the culture supernatants of a human fibroblast cell line crl1506 and a cloned epstein-barr virus (ebv) transformed human lymphoblastoid cell line (lcl) n23 were found to have il-6 activity by strongly promoting the growth, antibody secretion (increase of one- to three-fold), and cloning efficiencies of ...19938431556
susceptibility of human cells to puumala virus infection.nephropathia epidemica involves several organs including kidney, lung, liver and brain. to investigate the susceptibility of putative target cells to the agent responsible, puumala virus, we screened established human cell lines of lung (wi-38, a-427, ccd-11lu), kidney (a-704), liver (hep g2), pharynx (detroit 562), submaxillary gland (a-253) and neural (sk-n-mc, sh-sy5y) origin as well as primary human kidney glomerular cells, endothelial cells and peripheral blood monocytes/macrophages. propag ...19938445370
rna binding of recombinant nucleocapsid proteins of hantaviruses.genes encoding the nucleocapsid (n) proteins of two hantaviruses, hantaan virus strain 76-118 (htn) and puumala virus strain cg 18-20 (puu), were expressed in escherichia coli as histidine-tagged proteins. they were purified by metal-chelate affinity chromatography under native or denaturing conditions to near homogeneity. the soluble form of htn n protein was associated with rna of e. coli. renatured n proteins were shown to bind in vitro transcribed rna representing the hantaviral small genomi ...19938480424
dobrava virus as a new hantavirus: evidenced by comparative sequence analysis.dobrava virus, recently isolated from a yellow-neck mouse (apodemus flavicollis), captured in a northern slovenian village where severe cases of hemorrhagic fever with renal syndrome were recognized, was shown by serology and restriction enzyme digestion of pcr-amplified gene segments to be related to previously recognized hantaviruses. to investigate further the relationship of this new isolate to other hantaviruses, a portion of the medium (m) genome segment of dobrava virus was amplified by p ...19938487037
outbreak of acute illness--southwestern united states, 1993.beginning in may 1993, cases of acute illness characterized by fever, myalgias, headache, and cough, followed by rapid development of respiratory failure, have been reported to the new mexico department of health (nmdoh), arizona department of health services (adhs), colorado department of health (cdh), and utah department of health (udh). this report presents preliminary findings from an ongoing investigation of this problem, which suggest this illness is associated with a previously unrecogniz ...19938502213
update: outbreak of hantavirus infection--southwestern united states, 1993.since may 1993, the new mexico department of health, the arizona department of health services, the colorado department of health, the utah department of health, the indian health service (ihs), and cdc, with the assistance of the navajo nation division of health, have been investigating an outbreak of acute illness characterized by a prodrome most commonly including fever, myalgias, headache, and cough, followed rapidly by respiratory failure. preliminary laboratory findings have suggested this ...19938502218
microbes ever marching. 19938505964
from the centers for disease control and prevention. infectious diseases update: outbreak, hantavirus infection--southwestern united states, 1993. 19938510287
update: outbreak of hantavirus infection--southwestern united states, 1993.since may 1993, the new mexico department of health, the arizona department of health, the colorado department of health, the utah department of health, the indian health service, and cdc, with the assistance of the navajo nation division of health, have been investigating an outbreak of illness associated with hantavirus infection. this report updates information regarding the relation between illness and infection with a previously unrecognized hantavirus.19938510634
update: outbreak of hantavirus infection--southwestern united states, outbreak of illness associated with hantavirus infection continues to be investigated by state health departments in new mexico, arizona, colorado, and utah; the indian health service; and cdc, with the assistance of the navajo nation division of health. this report updates information regarding the outbreak and presents information on two cases that occurred in the 10 months preceding this outbreak.19938510636
update: hantavirus infection--united states, 1993. 19938515743
protective immunity of hantaan virus nucleocapsid and envelope protein studied using baculovirus-expressed proteins.recombinant hantaan virus nucleocapsid protein (rnp) and recombinant envelope (renv) proteins were prepared using a baculovirus expression system to examine the role of hantaan virus structural proteins in protective immunity. passive transfer of spleen cells from mice immunized with rnp conferred partial protection or prolongation of time to death from fatal hantaan virus infection in suckling mice which were challenged with hantaan virus at 40 ld50 (survival rate: 43%) or 4 ld50 (survival rate ...19938517793
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