Publications

community-acquired pneumonia: new facets of an old disease--hantavirus pulmonary syndrome.	it seems that with climatic and geoecologic changes, hantaviruses have re-emerged as human pathogens related to increases in interaction between humans and rodent reservoirs. infection with snv in north america and the andes virus in south america can produce infection manifest initially as a flu-like illness. in the setting of a history of possible exposure to rodents or their excreta, clinical symptoms and laboratory clues such as thrombocytopenia should raise the suspicion of hps. clinical de ...	2005	15763221
[landscape ecology of hantavirosis in rio grande do sul state].	the aim of this work was to study the landscape ecology of hantavirosis in rio grande do sul state, brazil. this was achieved through geocoding the occurrence of cases and overlaying onto vegetation and relief maps. the majority of cases occurred during spring, in highland areas dominated by secondary vegetation and agricultural activity.	2004	15765604
human and rodent humoral immune responses to andes virus structural proteins.	in the present work we identified b-cell epitopes recognized by sera of humans and rodents naturally infected with andes virus, a hantavirus present in chile and argentina. analysis of patient and rodent sera with overlapping peptides revealed 21 human and rodent epitopes on the three structural proteins. whereas in the nucleoprotein the region comprising aa 248-260 was shown to be the key determinant of human sera, the major antigenic site of rodent antibody reactivity is located at aa 326-338. ...	2005	15780882
[increase of hantavirus infections in france, 2003].	hantaviral infections causing hemorrhagic fever with renal syndrome are endemic in north eastern france. humans are contaminated by the inhalation of aerosols contaminated by rodent faeces. in february 2003, the national reference centre (nrc) for hemorrhagic fevers detected an increased number of cases. an investigation was carried out to confirm the outbreak and take appropriate control measures.	2005	15780895
[outbreak of hemorrhagic fever with renal syndrome in the ulyanovsk region].		2005	15801222
newly recognized hantaviruses associated with hantavirus pulmonary syndrome in northern brazil: partial genetic characterization of viruses and serologic implication of likely reservoirs.	following the occurrence of the first laboratory-confirmed cases of hantavirus pulmonary syndrome (hps) in maranhao state, brazil, rodents were trapped and rodent materials screened by elisa for antibodies to sin nombre and andes hantaviruses. antibody-positive samples were tested by rt-pcr, amplified products were sequenced, and phylogenetic trees were constructed for comparison with known hantaviruses. from 104 rodent blood samples collected (40 bolomys lasiurus, 52 holochilus sciureus, 12 oli ...	2005	15815145
clinical sin nombre hantaviral infections in children.		2005	15818300
epizootiology of sin nombre and el moro canyon hantaviruses, southeastern colorado, 1995-2000.	sin nombre virus (snv) is an etiologic agent of hantavirus pulmonary syndrome. to better understand the natural history of this virus we studied population dynamics and temporal pattern of infection of its rodent hosts in southeastern colorado (usa) from 1995 to 2000. we present evidence for the presence of two hantaviruses, snv in deer mice (peromyscus maniculatus) and el moro canyon virus in western harvest mice (reithrodontomys megalotis), at our study sites. sin nombre virus appeared only sp ...	2005	15827206
detection of human hantavirus infections in lithuania.	in europe certain hantaviruses are known to cause hemorrhagic fever with renal syndrome of different severity. the objective of the present investigation was to study the presence of hantavirus infections in lithuania.	2005	15827873
hfrs causing hantaviruses do not induce apoptosis in confluent vero e6 and a-549 cells.	hantaviruses are known to cause little or no cytopathic effect in vitro, but have been suggested to cause apoptosis. to determine whether different hantaviruses would induce apoptosis to varying degrees, confluent vero e6 cells were infected with the hemorrhagic fever with renal syndrome (hfrs) causing viruses hantaan, dobrava, saaremaa, and puumala. however, no difference was found in the percentage of adherent cells, or of cells with condensed nuclei, between non-infected and virus-infected ce ...	2005	15834879
ribavirin trials and hantavirus--what we should not conclude.		2005	15844085
[hantavirus infections].	hantaviruses are cosmopolite anthropozoonosis considered as an emerging disease. four pathogenic types for humans and part of the bunyaviridae species are hosted by rodents and have been isolated: the sin nombre virus responsible for the severe american respiratory form; the hantaan and seoul viruses responsible for hemorrhagic fevers with renal syndrome (hfrs) of severe to moderate expression in asia and also in the balkans; the puumala virus responsible for hfrs of moderate expression or the s ...	2005	15859577
acute sin nombre hantavirus infection complicated by renal failure requiring hemodialysis.		2005	15887441
hantavirus infection in thailand: first clinical case report.	this study involved 115 cases of fever of unknown origin (fuo) in patients who were admitted to the department of medicine, siriraj hospital from may 1999 to november 2000. among the patient sera screened by elisa for igg hantavirus, five were positive for igg hantavirus-reacting antibodies and eight tested positive for igm hantavirus-reacting antibodies. one serum had both igg and igm antibodies. the patient exhibited acute encephalitic febrile illness, thrombocytopenia, high ast and alt levels ...	2005	15906672
development and evaluation of serological assays for detection of human hantavirus infections caused by sin nombre virus.	the hantavirus cardiopulmonary syndrome (hcps) was first recognized in 1993 after a cluster of acute respiratory distress syndrome deaths in the southwestern of the united states. the major causative agent of hcps in north america is the sin nombre virus (snv) carried by the deer mouse peromyscus maniculatus. the first hcps case imported to europe was reported in 2002.	2005	15911448
[nephropathia caused by hantavirus puumala: a case report].	we present a patient from germany with hantavirus infection, admitted in the emergency room of our hospital, with fever, thrombocytopenia, acute renal failure, oliguria, mild proteinuria and hematuria. percutaneous renal biopsy revealed an acute interstitial nephritis without medulla haemorrhages. the virus infection confirmation was made by detection of igm against hantavirus puumala. this infection should be considered in patients with thrombocytopenia, fever and acute renal failure, over all ...	2005	15912658
[emerging respiratory infections caused by pneumotropic viruses].		2005	15915382
a hantavirus nucleocapsid protein segment exposed on hepatitis b virus core particles is highly immunogenic in mice when applied without adjuvants or in the presence of pre-existing anti-core antibodies.	hepatitis b virus (hbv) core particles carrying the amino-terminal 120 amino acids (aa) of the nucleocapsid (n) protein of the hantaviruses dobrava, hantaan or puumala have been demonstrated to be highly immunogenic in mice when complexed with adjuvants. here we demonstrate that even without adjuvant, these chimeric particles induced high-titered, and strongly cross-reactive n-specific antibody responses in balb/c and c57bl/6 mice. the induced n-specific antibodies represented all igg subclasses ...	2005	15917119
peptide antagonists that inhibit sin nombre virus and hantaan virus entry through the beta3-integrin receptor.	specific therapy is not available for the treatment of hantavirus cardiopulmonary syndrome caused by sin nombre virus (snv). the entry of pathogenic hantaviruses into susceptible human cells is dependent upon expression of the alpha(v)beta(3) integrin, and transfection of human beta(3) integrin is sufficient to confer infectibility onto cho (chinese hamster ovary) cells. furthermore, pretreatment of susceptible cells with anti-beta(3) antibodies such as c7e3 or its fab fragment reopro prevents h ...	2005	15919886
inactivation of hantaan virus-containing samples for subsequent investigations outside biosafety level 3 facilities.	the potential risk of accidental infection by hantaviruses in a clinical or research laboratory necessitates special precautionary measures. a biosafety program must address handling and disposal of infectious materials as well as appropriate virus inactivation or depletion procedures to permit necessary further processing of specimens outside the biosafety level 3 laboratory.	2005	15920350
low seroprevalence among farmers from nebraska and vicinity suggests low level of human exposure to sin nombre virus.		2005	15927920
variability of g1 gene of hantaviruses occurring in the hubei province, p.r. china from 1985 to 2000.	we studied variability of g1 gene of hantaviruses occurring in the hubei province, p.r. china. serum samples were collected from 229 patients with hemorrhagic fever with renal syndromes (hfrs) during 1985--1989 and 1996--2000 and were tested by rt-pcr for the presence of hantaan and seoul viruses (htnvs, seovs) and by restriction fragment length polymorphism (rflp) analysis for the respective pattern. out of 229 sera 166 (72.5%) were hantavirus-positive by rt-pcr, including 124 from 1985--1989 a ...	2005	15929399
hantavirus nucleocapsid protein: a multifunctional molecule with both housekeeping and ambassadorial duties.	in recent years important progress has been made studying the nucleocapsid (n) protein of hantaviruses. the n protein presents a good example of a multifunctional viral macromolecule. it is a major structural component of a virion that encapsidates viral rna (vrna). it also interacts with the virus polymerase (l protein) and one of the glycoproteins. on top of these "house keeping" duties, the n protein performs interactive "ambassadorial" functions interfering with important regulatory pathways ...	2005	15931462
central european dobrava hantavirus isolate from a striped field mouse (apodemus agrarius).	dobrava virus (dobv) is a hantavirus that causes hemorrhagic fever with renal syndrome (hfrs) in europe. it is hosted by at least two rodent species, apodemus flavicollis and a. agrarius. according to their natural hosts they form the distinct genetic lineages dobv-af and dobv-aa, respectively. we have now established a dobv isolate named slovakia (sk/aa) from an a. agrarius animal captured in slovakia. the complete s and m and partial l segment nucleotide sequences of the new isolate were deter ...	2005	15956394
clinical survey of hantavirus in southern brazil and the development of specific molecular diagnosis tools.	hantavirus pulmonary syndrome (hps) is an emerging disease caused by an increasing number of distinct hantavirus serotypes found worldwide. it is also a very severe immune disease. it progresses quickly and is associated with a high mortality rate. at the prodrome phase, hantavirosis symptoms can resemble those of other infectious diseases such as leptospirosis and influenza. thus, prognosis could be improved by developing a rapid and sensitive diagnostic test for hantavirus infection, and by im ...	2005	15964966
hantaviruses in central south america: phylogenetic analysis of the s segment from hps cases in paraná, brazil.	we sequenced the complete s segments of hantaviruses detected from 12 hps patients living in southern of brazil. samples were obtained from patients diagnosed in different years, in distinct areas, and with a broad spectrum of clinical signs. despite these differences, all the s proteins of hantavirus from paraná were identical, except for one amino acid substitution. phylogenetic analyses of the complete s segment nucleotide and amino acid sequences indicated that hantaviruses from paraná form ...	2005	15977228
hanta hemorrhagic fever with renal syndrome: a case report and review.	hantavirus infection is seldom reported in taiwan. the spectrum of clinical severity ranges from mild to severe and may cover hantavirus pulmonary syndrome and hemorrhagic fever with renal syndrome (hfrs). we report a case of hfrs in a 24-year-old soldier presenting with fever, chills, abdominal pain and generalized myalgia. his clinical course progressed through febrile, hypotensive, oliguria and polyuria phases. hantavirus infection was proven by serology at the second hospital week. the patie ...	2005	15986074
cross-species transfer of viruses: implications for the use of viral vectors in biomedical research, gene therapy and as live-virus vaccines.	all living organisms are continuously exposed to a plethora of viruses. in general, viruses tend to be restricted to the natural host species which they infect. from time to time viruses cross the host-range barrier expanding their host range. however, in very rare cases cross-species transfer is followed by the establishment and persistence of a virus in the new host species, which may result in disease. recent examples of viruses that have crossed the species barrier from animal reservoirs to ...	2005	15986492
[expression and genetic immunization of hantaan virus g2 recombinant adenovirus].	to express hantaan virus(htnv) envelope glycoprotein g(2) recombinant adenovirus(adeno-g(2)) in vero e6 cells and explore its property of inducing immune response.	2005	15989780
diffusion and home range parameters from rodent population measurements in panama.	simple random walk considerations are used to interpret rodent population data collected in hantavirus-related investigations in panama regarding the short-tailed cane mouse, zygodontomys brevicauda. the diffusion constant of mice is evaluated to be of the order of (and larger than) 200 meters squared per day. the investigation also shows that the rodent mean square displacement saturates in time, indicating the existence of a spatial scale which could, in principle, be the home range of the rod ...	2005	15998498
distribution and abundance of sigmodontine rodents in relation to hantavirus in neuquén, argentina.	in order to estimate spatial distribution, temporal variation, and prevalence of andes hantavirus antibody in the rodent community, and especially in oligoryzomys longicaudatus populations, four different ecosystems were trapped seasonally between spring 2001 and winter 2002 in neuquen, northwestern argentinean patagonia. five peridomestic settings were sampled within the same period. the rodent o. longicaudatus had the widest distribution in neuquen, as it was the only species captured at every ...	2005	16007965
a preliminary study of the patterns of sin nombre viral infection and shedding in naturally infected deer mice (peromyscus maniculatus).	deer mice (peromyscus maniculatus) were trapped in southern manitoba, canada and tested for evidence of sin nombre virus infection. viral genome was amplified from tissues as well as saliva/oropharyngeal fluid, and urine samples were collected from seropositive animals. detection of viral rna in tissue samples and excreta/secreta from mice suggest that differences may exist between naturally infected rodents with respect to viral shedding.	2005	16011428
state-by-state incidences of hantavirus pulmonary syndrome in the united states, 1993-2004.	we investigated geographic disparity in numbers of hantavirus pulmonary syndrome (hps) cases in the united states. the 12-year incidences of hps (cases/100,000 for total and rural residents) by state did not parallel the number of cases per state. the state with the greatest overall incidence was new mexico, with montana ranking second. when rural incidence based on rural human population sizes were compared, new mexico also had the highest incidence, but utah, nevada, montana, arizona, and colo ...	2005	16011436
essential amino acids of the hantaan virus n protein in its interaction with rna.	the nucleocapsid (n) protein of hantavirus encapsidates viral genomic and antigenomic rnas. previously, deletion mapping identified a central, conserved region (amino acids 175 to 217) within the hantaan virus (htnv) n protein that interacts with a high affinity with these viral rnas (vrnas). to further define the boundaries of the rna binding domain (rbd), several peptides were synthesized and examined for the ability to bind full-length s-segment vrna. peptide 195-217 retained 94% of the vrna ...	2005	16014963
[the renal failure and capillary leak during the acute stage of (dobrava) dob and puu (puumala) infection].	the target organ for puumala (puu) and dobrava (dob) viruses is the kidney, but the impact on renal function seems far more important in dob infection than in puu infections. aim of study was to estimate the renal failure and capillary leak during the acute stage of dob-infection and puu-infection. 50 patients with serological conformed diagnosis of acute hantavirus infection were included in the study. diuresis, serum urea and creatinine were measured. size of kidneys and presence of ascites wa ...	2005	16018388
hantavirus: 'tis the season.		2005	16027428
social status does not predict responses to seoul virus infection or reproductive success among male norway rats.	trade-offs exist among life history strategies that are used to increase survival and reproduction; such that, males that engage in more competitive behaviors may be more susceptible to infection. hantaviruses are transmitted horizontally between rodents through the passage of virus in saliva during wounding and male rodents are more likely to be infected with hantaviruses than females. to determine whether a trade-off exists between dominance and susceptibility to seoul virus infection, male lo ...	2006	16040226
hemorrhagic fever with renal syndrome in the pomurje region of slovenia--an 18-year survey.	to determine the etiology of hemorrhagic fever with renal syndrome (hfrs) in the north-eastern part of slovenia (pomurje region) together with demographic, epidemiological and clinical data on 25 patients from this region who were diagnosed and treated at the general hospital in murska sobota between 1986 and 2003.	2005	16053195
quality assurance for the diagnostics of viral diseases to enhance the emergency preparedness in europe.	the threat posed by emerging and re-emerging communicable diseases and, more recently, by the intentional release of infectious agents in a susceptible population, has been receiving considerable attention at the national and international levels. public health efforts to strengthen disease detection, surveillance and control have been intensified. however, clinicians and clinical microbiology laboratories play an important role in the early detection of disease, the identification of the putati ...	2005	16077216
[the epizootological and virological characteristics of a natural hantavirus infection focus in the subtropic zone of the krasnodarsk territory].	a natural focus of hantavirus infection was detected and examined during the studies conducted in 2000-2002 around the sochi (the western spurs of the great caucasus ridge, which descended to the black sea (the krasnodar territory of russia). at least 4 rodent species, such as microtus majori, a. (s.) ponticus, a. agrarius, a. (s.) ciscaucasicus, were shown to participate in the circulation of hantaviruses. a comparative analysis of the nucleotide sequences of genomic s- and m-segments of hantav ...	2005	16078428
hantavirus pulmonary syndrome in the americas: the early years.	the initial recognition of hantavirus pulmonary syndrome (hps) as a new disease associated with a cluster of acute respiratory deaths among american indians in the southwestern united states in 1993 bears little resemblance to the current understanding of this syndrome. hps is now recognized as a zoonotic disease that has been endemic throughout the americas for at least 40 years and that is closely linked to population densities and virus dynamics among a specific subfamily of rodents. the clas ...	2000	16088742
[diagnostic tests: hantavirus].		2005	16111261
hantavirus infection in thailand: first clinical case report.	this study involved 115 cases of fever of unknown origin (fuo) patients who were admitted to the department of medicine, siriraj hospital from may 1999 to november 2000. among the patient sera screened by elisa for igg hantavirus, five were positive for igg hantavirus-reacting antibodies and eight tested positive for igm hantavirus-reacting antibodies. one serum had both igg and igm antibodies. the patient exhibited acute encephalitic febrile illness, thrombocytopenia, high ast and alt levels, a ...	2005	16124441
replication and immunoactivity of the recombinant peromyscus maniculatus cytomegalovirus expressing hantavirus g1 glycoprotein in vivo and in vitro.	previously, we have shown that cmv isolated from deer mouse could be used in vivo and in vitro to express sin nombre virus (snv) glycoprotein g1 in deer mice [rizvanov aa, van geelen ag, morzunov s, et al. generation of a recombinant cytomegalovirus for expression of a hantavirus glycoprotein. j.virol. 2003;77(22):12203-10]. in this report, we further characterize replication of wild-type (wt) and recombinant peromyscus cmv (pcmv) in vivo and in vitro using realtime pcr, and infectious center as ...	2006	16125285
[infection due to hantavirus and other rodent-borne viruses].	the term "robovirus" (rodent-borne virus) refers to viruses belonging to the bunyaviridae (genus hantavirus) and arenaviridae families, which are occasionally transmitted to human beings from rodents, their natural hosts. hantaviruses cause two human diseases: hemorrhagic fever with renal syndrome and hantavirus pulmonary syndrome. arenaviruses produce hemorrhagic fevers or acute central nervous system disease in humans. this article reviews the biology, epidemiology, pathogenesis, clinical feat ...	2005	16185565
norway rat population in baltimore, maryland, 2004.	norway rats are reservoirs for several zoonotic agents, including hantaviruses, and are implicated in the transmission of pathogens to humans in urban environments. the rat population of baltimore, maryland was estimated from surveys in 1949 and again in 1952, but has not been evaluated for more than 50 years. previously identified sociodemographic risk factors for rat infestation, including median income, human density, and percentage of rental properties, were used to categorize census block g ...	2005	16187901
[analysis of part of m gene and genotyping for m segment of hantavirus detected from hfrs patients' sera in qingdao region during 2000 - 2003].	to study the molecular epidemiological characteristics of hantavirus seen during 2000-2003 in qingdao region of shandong province.	2005	16201465
[genotype of isolated strain of hantavirus in liaoning area].	to isolate hantavirus and to genotype isolated strains of hantavirus (sy 13) in liaoning province.	2005	16201470
a retrospective study of hantavirus infection in kinmen, taiwan.	in this retrospective study, 140 serum samples collected from 85 scrub typhus-negative patients in kinmen island in 2000 were tested for antibodies to hantavirus using enzyme-linked immunosorbent assay. seven patients (8.23%) were confirmed as having hantavirus infection as demonstrated by increased hantavirus-specific immunoglobulin m and/or immunoglobulin g antibodies in their convalescent serum samples. analysis of indirect immunofluorescence assay showed that seoul type was the etiologic age ...	2005	16211143
a mathematical model for indirectly transmitted diseases.	we consider a mathematical model for the indirect transmission via a contaminated environment of a microparasite between two spatially distributed host populations having non-coincident spatial domains. the parasite is benign in a first population and lethal in the second one. global existence results are given for the resulting reaction-diffusion system coupled with an ordinary differential equation. then, invasion and persistence of the parasite are studied. a simplified model for the transmis ...	2007	16216284
hantavirus gc glycoprotein: evidence for a class ii fusion protein.	hantavirus cell entry is promoted by its envelope glycoproteins, gn and gc, through cell attachment and by fusion between viral and endosomal membranes at low ph. however, the role of gn and gc in receptor binding and cell fusion has not yet been defined. in this work, a sequence presenting characteristics similar to those of class ii fusion peptides (fps) of alphavirus e1 and flavivirus e proteins is identified within the hantavirus gc glycoprotein. a three-dimensional comparative molecular mod ...	2005	16227214
long-term dynamics of sin nombre viral rna and antibody in deer mice in montana.	infections with hantaviruses in the natural host rodent may result in persistent, asymptomatic infections involving shedding of virus into the environment. laboratory studies have partially characterized the acute and persistent infection by sin nombre virus (snv) in its natural host, the deer mouse (peromyscus maniculatus). however, these studies have posed questions that may best be addressed using longitudinal studies involving sequential sampling of individual wild-caught, naturally infected ...	2005	16244056
are humans infected by hantaviruses in australia?	hantavirus antibody-positive rodents have been found across australia although, to date, there are no reports of infections in humans. this could be due to misdiagnosis clinically and/or inadequate laboratory technique/skills. there are close trading ties between australia and asian countries as well as our geographical neighbours where both human and rodent infections are found, so importation is a continuing threat. we consider that further sero-epidemiological surveys are warranted among rode ...	2005	16248862
hantaviruses are here in india.		2005	16251773
a pilot study for serological evidence of hantavirus infection in human population in south india.	hantaviruses are rodent-borne viruses of the family bunyaviridae that have been identified as aetiological agents of two human diseases, haemorrhagic fever with renal syndrome (hfrs) and hantavirus pulmonary syndrome (hps). there are no reports of hantavirus infections in humans from india, hence this pilot study was undertaken to provide the serological evidence of hantavirus infections in humans in south india.	2005	16251777
metabolic activity of macrophages infected with hantavirus, an agent of hemorrhagic fever with renal syndrome.	monocytes/macrophages are thought to play an important role in pathogenesis of viral infections. these cells are involved in distribution and persistence of viruses in the organism and also influence the regulation of immune reactions. the functional and enzymatic activities of macrophages infected with an agent of hemorrhagic fever with renal syndrome were analyzed for the first time. this disease is caused by a virus of the hantavirus genus, the bunyaviridae family. activities of ectoenzymes 5 ...	2005	16266269
hantavirus infection.		2005	16267154
[rodents as the vectors of infective agents in the natural foci of infections in the moscow megapolis].	this work deals with the results of 40-year observations on the circulation of infective agents in the natural foci of infections (tularemia, leptospirosis, hemorrhagic fever with renal syndrome) among rodents and other small mammals in the territory of the moscow. the monitoring of their frequency and the infection rates remains the main effective measure for the prophylaxis of dangerous infections among the population of the megapolis.	2005	16279545
double-stranded rna induces the synthesis of retinoic acid-inducible gene-i in vascular endothelial cells.	viral infection induces various responses in vascular endothelial cells. polyinosinic-polycytidylic acid (poly ic) is a synthetic double-stranded rna (dsrna), and treatment of cells with poly ic mimics the viral infection to the cells. retinoic acid-inducible gene-i (rig-i) is a protein belonging to the dexh-box family and designated as a putative rna helicase. rig-i is considered to play a role in antiviral responses through the regulation of gene expressions. in the present study, the authors ...	2005	16291516
induction of innate immune response genes by sin nombre hantavirus does not require viral replication.	maladaptive immune responses are considered to be important factors in the pathogenesis of the two diseases caused by hantaviruses, hemorrhagic fever with renal syndrome and hantavirus cardiopulmonary syndrome (hcps). while the intensity of adaptive antiviral t-cell responses seems to correlate with the severity of hcps, there is increasing evidence that innate antiviral responses by endothelial cells, the native targets for hantavirus infection in vivo, are induced within hours of exposure to i ...	2005	16306571
cellular entry of hantaan virus a9 strain: specific interactions with beta3 integrins and a novel 70kda protein.	cellular entry of pathogenic hantaviruses had been shown to be mediated by beta3 integrins. however, no direct evidence exists that hantavirus binds to beta3 integrins, and integrin beta3 subunit is not expressed on some cells permissive to hantavirus infection. in this report, utilizing beta3-integrin-transfected cho cells, we demonstrated that integrin beta3 subunit renders cho cells susceptible to chinese hantaan virus (htn) strain a9 (isolated in china), and the viral infection was correspon ...	2006	16310165
phylogenetic evidence for the distinction of saaremaa and dobrava hantaviruses.	dobrava virus (dobv) and saaremaa virus (saav) are two closely related hantaviruses carried by different rodent species. the distinction of these two viruses has been a matter of debate. while the phylogenies based on the viral m segment sequences were repeatedly showing monophyly of saav strains, some trees based on the s segment sequences were not, thus causing questions on the demarcation between these two viruses. in order to clarify this issue, the current collection of the virus s segment ...	2005	16336658
[addition of an arterio-venous shunt during veno-arterial extracorporeal life support in a patient with hantavirus pulmonary syndrome].	a subgroup of patients infected with the hantavirus develops a pulmonary syndrome (hps) characterized by severe acute respiratory failure and myocardial depression, that has a high mortality rate. extracorporeal life support (ecls) could be a valuable therapeutic tool in such patients. we report a 24 years old male with hps that was successfully managed when an arterio-venous shunt was added to a conventional veno-arterial ecls technique. precise criteria have been developed to predict which pat ...	2005	16341389
a longitudinal study of bayou virus, hosts, and habitat.	what is currently known about the ecology of north american hantaviruses has come largely from studies on sin nombre virus (snv). we conducted a longitudinal study of bayou virus (bayv), the second-leading agent of hantavirus pulmonary syndrome in the united states. antibodies to hantavirus were detected from oryzomys palustris (most commonly infected species), sigmodon hispidus, peromyscus leucopus, reithrodontomys fulvescens, and baiomys taylori. however, only o. palustris had viral rna in tis ...	2005	16354810
regulation of rodent-borne viruses in the natural host: implications for human disease.	prevalence and transmission rates of rodent-borne viruses within host populations vary in time and space and among host-virus systems. improving our understanding of the causes of these variations will lead to a better understanding of changes in disease risk to humans. the regulators of prevalence and transmission can be categorized into five major classes: (1) environmental regulators such as weather and food supply affect transmission rates through their effect on reproductive success and pop ...	2005	16355867
first evidence of fatal hantavirus nephropathy in india, mimicking leptospirosis.		2006	16361279
[hantavirus infection].	hemorrhagic fever with renal syndrome (hfrs) and hantavirus pulmonary syndrome (hps) are rodent borne viral zoonosis caused by hantavirus infection. hfrs and hps cause epidemic in eurasia continent and north and south american continent, respectively. various species of wild rodents play as a reservoir animal in nature. laboratory rat derived hfrs epidemic has also been reported in several countries including japan. recently, hfrs and hps were classified to category 4 diseases in the japanese la ...	2005	16363694
hantavirus and arenavirus antibody prevalence in rodents and humans in trentino, northern italy.	the spatial and temporal distribution of hantavirus and arenavirus antibody-positive wild rodents in trentino, italy, was studied using immunofluorescence assays (ifa) in two long-term sites trapped in 2000-2003, and six other sites trapped in 2002. the overall hantavirus seroprevalence in the bank voles, clethrionomys glareolus (n=229) screened for puumala virus (puuv) antibodies was 0.4%, and that for apodemus flavicollis mice (n=1416) screened for dobrava virus (dobv) antibodies was 0.2%. ant ...	2006	16371172
soochong virus: an antigenically and genetically distinct hantavirus isolated from apodemus peninsulae in korea.	hantaan (htn) virus, the etiologic agent of clinically severe hemorrhagic fever with renal syndrome (hfrs), was first isolated in 1976 from lung tissue of a striped-field mouse (apodemus agrarius) captured in songnae-ri, gyeonggi province, republic of korea. found primarily in mountainous areas, the korean field mouse (a. peninsulae) is the second-most dominant field rodent species found throughout korea. a new hantavirus, designated soochong (soo), was isolated in vero e6 cells from four a. pen ...	2006	16372283
hantaviruses: molecular biology, evolution and pathogenesis.	hantaviruses are tri-segmented negative sense single stranded rna viruses that belong to the family bunyaviridae. in nature, hantaviruses are exclusively maintained in the populations of their specific rodent hosts. in their natural host species, hantaviruses usually develop a persistent infection with prolonged virus shedding in excreta. humans become infected by inhaling virus contaminated aerosol. unlike asymptomatic infection in rodents, hantaviruses cause two acute febrile diseases in human ...	2005	16375712
rift valley fever virus.	rift valley fever is considered to be one of the most important viral zoonoses in africa. in 2000, the rift valley fever virus spread to the arabian peninsula and caused two simultaneous outbreaks in yemen and saudi arabia. it is transmitted to ruminants and to humans by mosquitoes. the viral agent is an arbovirus, which belongs to the phlebovirus genus in the bunyaviridae family. this family of viruses comprises more than 300 members grouped into five genera: orthobunyavirus, phlebovirus, hanta ...	2005	16375716
hantaan/andes virus dna vaccine elicits a broadly cross-reactive neutralizing antibody response in nonhuman primates.	at least four hantavirus species cause disease with prominent renal involvement-hemorrhagic fever with renal syndrome (hfrs); and several hantavirus strains cause disease with significant pulmonary involvement-hantavirus pulmonary syndrome (hps). the most prevalent and lethal hantaviruses associated with hfrs and hps are hantaan virus (htnv) and andes virus (andv), respectively. here, we constructed a dna vaccine plasmid (pwrg/ha-m) that contains both the htnv and andv m gene segments. rhesus ma ...	2006	16378630
[genetics subtypes and distribution of seoul virus in henan].	to investigate the sub-genotypes and distribution ot seoul virus in henan.	2005	16390005
[hantavirus nephropathy].		2005	16392314
bidirectional virus secretion and nonciliated cell tropism following andes virus infection of primary airway epithelial cell cultures.	hantavirus pulmonary syndrome (hps) is an acute disease resulting from infection with any one of a number of new world hantaviruses. hps has a mortality rate of 40% and, unlike many other severe respiratory diseases, often occurs in young, healthy adults. infection is usually initiated after inhalation of rodent excreta containing virus particles, but human-to-human transmission has been documented. postmortem tissue samples show high levels of viral antigen within the respiratory endothelium, b ...	2006	16414986
[a clinical study on cd178 positive t lymphocyte in hemorrhagic fever with renal syndrome].	to further probe into the role of cd178 in the pathogenesis of hemorrhagic fever with renal syndrome (hfrs).	2005	16416001
habitat factors associated with bank voles (clethrionomys glareolus) and concomitant hantavirus in northern sweden.	puumala virus (puuv), genus hantavirus, causes nephropathia epidemica, a mild form of hemorrhagic fever with renal syndrome in humans. in this study, bank voles, the natural reservoir of puuv, were captured at locations of previous human puuv exposure and paired controls within a region of high incidence in northern sweden. the aim of the study was to evaluate the influence of environmental factors on the abundance of bank voles and the occurrence of puuv. the total number of voles and the numbe ...	2005	16417427
a pseudotype vesicular stomatitis virus containing hantaan virus envelope glycoproteins g1 and g2 as an alternative to hantavirus vaccine in mice.	we examined whether a vesicular stomatitis virus (vsv) pseudotype bearing the hantavirus envelope glycoproteins (gps) g1 and g2 (vsvdeltaghtn) could be used as a safe and effective alternative to native hantavirus. mice were immunized with purified particles of vsvdeltaghtn. after the second immunization, all mice produced anti-gp antibody as detected in elisa and a neutralization test. after the third immunization, the mice were challenged with hantaan virus. neither anti-np antibody producti ...	2006	16426712
serological assays for the detection of human andes hantavirus infections based on its yeast-expressed nucleocapsid protein.	the objective of the study was to develop and evaluate igm and igg elisas and an igg western blot test for the serological detection of human infections with andes virus (andv), the major cause of hantavirus cardiopulmonary syndrome (hcps) in south america.	2006	16428893
targeting vascular injury using hantavirus-pseudotyped lentiviral vectors.	restenosis is a pathological condition involving intimal hyperplasia and negative arterial remodeling. gene therapy vectors have shown modest therapeutic effects, but the level of infectivity has been relatively poor. in the present study we have designed a modified lentiviral vector (lv) pseudotyped with a strain of hantavirus (htnv) to improve the transduction efficiency into vascular smooth muscle and endothelial cells in vitro and in vivo. in vivo studies using adult new zealand white rabbit ...	2006	16431160
person-to-person transmission of andes virus.	despite the fact that rodents are considered to be the infectious source of hantavirus for humans, another route of transmission was demonstrated. andes virus (andv) has been responsible for most of the cases recorded in argentina. person-to-person transmission of andv sout lineage was described during an outbreak of hantavirus pulmonary syndrome in southwest argentina. in this study, we analyzed 4 clusters that occurred in 2 disease-endemic areas for different andv lineages. we found new eviden ...	2005	16485469
neutralizing antibodies in survivors of sin nombre and andes hantavirus infection.	we evaluated titers of homotypic and heterotypic neutralizing antibodies (nabs) to andes and sin nombre hantaviruses in plasma samples from 20 patients from chile and the united states. all but 1 patient had high titers of nab. none of the plasma samples showed high titers against the heterologous virus.	2006	16494739
a novel method for cloning of non-cytolytic viruses.	hantaviruses are rodent-borne pathogens with a segmented single-stranded rna genome of negative polarity. spontaneous occurrence of variants with genetic heterogeneity have been observed both in vivo and in vitro. the objective of this study was to establish a method for the cloning of genetically homogenous hantaviruses which can be used for subsequent functional studies. infected veroe6 cells were incubated with an agarose/medium overlay to prevent uncontrolled distribution of de novo synthesi ...	2006	16504312
microbiological contamination of laboratory mice and rats in korea from 1999 to 2003.	to survey the microbiological contamination of laboratory mice and rats in korea during a 5-year period, we monitored animals housed in mouse and rat facilities with either barrier or conventional systems. at barrier and conventional mouse facilities, the most important pathogen identified was mouse hepatitis virus (mhv), while mycoplasma pulmonis was the most important pathogen at conventional rat facilities. interestingly, hantavirus was recovered from both barrier and conventional mouse facil ...	2006	16508207
production of hantavirus puumala nucleocapsid protein in saccharomyces cerevisiae for vaccine and diagnostics.	the production of hantavirus puumala nucleocapsid (n) protein for potential applications as a vaccine and for diagnostic purposes was investigated with saccharomyces cerevisiae as a recombinant host. the n protein gene and the hexahistidine tagged n (h-n) protein gene were expressed intracellular from a 2-microm plasmid vectors under the control of a fused galactose inducible gal10-pyk promoter. for monitoring the recombinant gene expression, a h-n and a gfp fusion protein was used. different cu ...	2006	16513199
dobrava, but not saaremaa, hantavirus is lethal and induces nitric oxide production in suckling mice.	hantaviruses are the causative agents of hfrs and hcps (hemorrhagic fever with renal syndrome and hantavirus cardiopulmonary syndrome), two severe, and often fatal human diseases. mortality from hfrs varies between hantaviruses; hantaan and dobrava show the highest, seoul intermediate, and puumala low mortality. saaremaa, genetically closely related to dobrava, is also known to induce hfrs, with low or no mortality. in this study, mice were inoculated with dobrava and saaremaa viruses to test fo ...	2006	16513381
generation of monoclonal antibodies of desired specificity using chimeric polyomavirus-derived virus-like particles.	foreign protein sequences presented on hamster polyomavirus (hapyv) major capsid protein vp1-derived virus-like particles (vlps) have been demonstrated to be highly immunogenic. the current study was aimed to evaluate vp1-derived chimeric vlps as tools for hybridoma technology to generate monoclonal antibodies (mabs) of desired specificity. chimeric vlps containing inserts of different size and origin were used as immunogens. chimeric vlps carrying a 9 amino acid (aa)-long cytotoxic t-cell epito ...	2006	16516908
corticosteroids combined with continuous veno-venous hemodiafiltration for treatment of hantavirus pulmonary syndrome caused by puumala virus infection.	reported here are two cases of hantavirus pulmonary syndrome caused by puumala virus infection, which rapidly resolved after initiation of corticosteroid treatment combined with continuous veno-venous hemodiafiltration. these cases emphasize the role of the inflammatory response in the pathogenesis of hantavirus pulmonary syndrome.	2006	16550348
is there an association of pneumocystis infection with the presence of arena-, hanta-, and poxvirus antibodies in wild mice and shrews in finland?	as part of studies on the nature of the endemic virus infections in natural rodent hosts, the possible association of cyst forms of pneumocystis spp. with the presence of hanta-, cowpox-, and arenavirus antibodies in wild mice (apodemus flavicollis, n=105; apodemus agrarius, n=63; micromys minutus, n=50) and the common shrew (sorex araneus, n=101) was studied in south-central finland. one hantavirus (saaremaa virus, saav) seropositive a. agrarius, and 2 cowpoxvirus (cpxv) seropositive s. araneus ...	2006	16556344
which hantaviruses in india ?		2006	16567875
maternally derived recombinant human anti-hantavirus monoclonal antibodies are transferred to mouse offspring during lactation and neutralize virus in vitro.	transgenic mice expressing a recombinant human monoclonal antibody (rhmab) against hantavirus were generated. these mice could be used as models to explore the possibilities of producing rhmabs for therapeutic purposes. the highest concentration of the rhmab in the milk of the transgenic females was 6.6 mg/ml. the rhmab was also detected in the sera of pups fed by the transgenic females. both the rhmabs in the milk of transgenic mice and those in the sera of suckling pups were found to be active ...	2006	16571835
saaremaa hantavirus should not be confused with its dangerous relative, dobrava virus.		2006	16597913
biological control agents elevate hantavirus by subsidizing deer mouse populations.	biological control of exotic invasive plants using exotic insects is practiced under the assumption that biological control agents are safe if they do not directly attack non-target species. we tested this assumption by evaluating the potential for two host-specific biological control agents (urophora spp.), widely established in north america for spotted knapweed (centaurea maculosa) control, to indirectly elevate sin nombre hantavirus by providing food subsidies to populations of deer mice (pe ...	2006	16623730
ten-year prognosis of puumala hantavirus-induced acute interstitial nephritis.	nephropathia epidemica (ne) is a hemorrhagic fever with renal syndrome caused by puumala hantavirus. its long-term prognosis is considered favorable. there are, however, some reports about subsequent hypertension, glomerular hyperfiltration, and proteinuria after previous hantavirus infection. therefore, we studied 36 patients 5 and 10 years after acute ne, with 29 seronegative controls. office blood pressure, ambulatory 24-h blood pressure (abp), glomerular filtration rate (gfr), and proteinuri ...	2006	16641933
a friday afternoon case of apparent anti-glomerular basement nephritis.		2006	16644771
a new puumala hantavirus subtype in rodents associated with an outbreak of nephropathia epidemica in south-east germany in 2004.	a micro-epidemic of hantavirus infections occurred in lower bavaria, south-east germany, starting in april 2004. while only three cases were registered from 2001 to 2003, a dramatically increased number of clinically apparent human hantavirus infections (n=38) was observed in 2004, plus seven additional cases by june 2005. to determine the reservoir responsible for the infections, a total of 43 rodents were trapped in lower bavaria. serological and genetic investigations revealed that puumala vi ...	2006	16650330
[identification of infectious agents in cases suspicious of hantavirus infection but with negative serology].	the diagnosis of acute respiratory illness caused by hantavirus is based in the rapid and progressive clinical course, epidemiological background and the serological confirmation of the virus. when the presence of the virus is not confirmed a differential diagnosis must be made with other infections. between 1999 and 2001, the chilean public health institute received 1063 blood samples from patients with a suspicious clinical picture, to study the presence of andes strain of hantavirus. in 134 o ...	2006	16676106
the effect of biodiversity on the hantavirus epizootic.	we analyze a mathematical model of the epizootic of hantavirus in mice populations, including the effect of species that compete with the host. we show that the existence of the second species has an important consequence for the prevalence of the infectious agent in the host. when the two mice species survive in the ecosystem, the competitive pressure of the second species may lead to reduction or complete elimination of the prevalence of infection. the transition between the disappearance of t ...	2006	16676531
[viral hemorrhagic fevers in brazil].	to call attention to viral hemorrhagic fevers, diseases that are mostly underdivulged and, probably, underreported, we present here case reports of the 4 diseases of this kind that occur in brazil: yellow fever, dengue haemorrhagic fever/dengue shock syndrome, arenavirus haemorrhagic fever and hantavirus cardiopulmonary syndrome. relevant clinical, epidemiological and laboratorial diagnostic aspects of these viral haemorrhagic fevers are also shown here. these diseases have a high case fatality ...	2006	16699651
hantavirus in african wood mouse, guinea.	hantaviruses are rodent-borne, emerging viruses that cause life-threatening human diseases in eurasia and the americas. we detected hantavirus genome sequences in an african wood mouse (hylomyscus simus) captured in sangassou, guinea. sequence and phylogenetic analyses of the genetic material demonstrate a novel hantavirus species, which we propose to name "sangassou virus."	2006	16704849