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[identification of a strain of epidemic hemorrhagic fever (ehf) virus from patients' sera]. 19836134574
haemorrhagic fever with renal syndrome: memorandum from a who meeting.haemorrhagic fever with renal syndrome (hfrs) is a public health problem throughout most of the european and asian land mass. although predominantly associated with rural areas, it is now being recognized as an urban problem in some countries, and also presents a particular hazard to laboratory staff who use rodents for biomedical research. in wild rodents (rats, mice and voles) the infection is asymptomatic. human infection with the hfrs agent(s) is sporadic, but under special circumstances epi ...19836134591
immunofluorescence studies of disseminated hantaan virus infection of suckling mice.hantaan virus, the etiological agent of korean hemorrhagic fever, was inoculated intracerebrally or intraperitoneally into suckling mice, and the course of the infection was followed by infectivity titration and immunofluorescence studies. mice became ill and were moribund by 13 to 14 days postinfection. in mice inoculated either intracerebrally or intraperitoneally, virus antigen was present in brain, heart, lungs, liver, and kidney. less consistently, specific fluorescence was observed in sple ...19836134678
isolation of virus causing hemorrhagic fever with renal syndrome (hfrs) through a cell culture system.twenty-three rat lung specimens collected in outbreaks of hemorrhagic fever with renal syndrome (hfrs) in three medical institutions were inoculated onto the vero-e6 cell monolayers. after several blind passages, an agent growing serially in the cell cultures and reacting specifically with known hfrs-positive sera was isolated from two of these specimens. the two isolates were antigenically identical each other. the agent, named strain sr-11, was identified as the causative virus of hfrs by its ...19836134854
etiologic studies of epidemic hemorrhagic fever (hemorrhagic fever with renal syndrome).two strains of epidemic hemorrhagic fever (ehf) virus were isolated from the lung tissues of apodemus agrarius mice that were captured in an area where ehf is endemic. the strains were isolated by passages in a. agrarius mice from a nonendemic area. identification of the isolates by usual procedures was confirmed by repeated blind tests with coded sera. contamination with certain known viruses such as reovirus, adenovirus (types 3 and 7), and other pathogens, such as murine typhus rickettsiae an ...19836132949
viruses of classical and mild forms of haemorrhagic fever with renal syndrome isolated in china have similar bunyavirus-like morphology. 19836131283
[hemorrhagic fever with a renal syndrome in the maritime territory].the sporadic character of the occurrence of hemorrhagic fever with the renal syndrome in the primorye territory has been established. three rodent species have been found to be spontaneously infected with the causative agent of this disease. antibodies in patients with this disease are detected at its early stages and persist for a long time after convalescence. the possibility of the formation of a contingent immune to hemorrhagic fever, due to the persons who have had mild and asymptomatic for ...19836132506
[serological studies of convalescent hemorrhagic fever patients with renal syndrome]. 19836133030
hfrs after a wild rodent bite in the haute-savoie--and risk of exposure to hantaan-like virus in a paris laboratory. 19846142362
antigenic comparison of virus strains of mild and classical types of epidemic haemorrhagic fever isolated in china and adaptation of these to cultures of normal cells. 19846142363
isolation of the nephropathia epidemica agent in sweden. 19846143925
propagation of nephropathia epidemica virus in cell culture. 19846143926
[indirect hemagglutination test for indicating the hfrs virus and the laboratory diagnosis of the disease].conditions have first been developed for the preparation of erythrocyte immunoglobulin diagnostic preparation of erythrocyte immunoglobulin diagnostic preparation for hfrs virus which permits express detection of the specific virus antigen in suspensions of the lung tissue of rodents by means of the indirect hemagglutination test (iha) and laboratory diagnosis of the infection in humans by means of antibody detection by the indirect hemagglutination-inhibition test (ihai). the iha test is as sen ...19846143451
isolation of a virus related to hemorrhagic fever with renal syndrome from urban rats in a nonendemic area. 19846143783
hfrs outbreak associated with laboratory rats in uk. 19846144891
[serological confirmation of cases of hemorrhagic fever with renal syndrome in byelorussia]. 19846147043
[survey on serum hemorrhagic fever-renal syndrome antibody in normal population of zhenjiang prefecture]. 19846147237
scottish case of haemorrhagic fever with renal syndrome. 19846149371
serological evidence for hantaan-related virus in africa. 19846149414
haemorrhagic fever virus with renal syndrome in small rodents in czechoslovakia.the antigen of haemorrhagic fever with renal syndrome (hfrs) was detected in the lungs of the following free-living small rodents trapped in different localities in eastern slovakia: clethrionomys glareolus (2 positive samples of 7), apodemus flavicollis (1 sample of 24) and apodemus agrarius (7 positive samples of 66). the virus was first identified by indirect fluorescent antibody (fa) staining using human convalescent serum from a case of epidemic nephropathy (ne) of scandinavia. the lung sus ...19846151356
[natural foci of hemorrhagic fever with renal syndrome in jiangsu province]. 19846152406
isolation and propagation of nephropathia epidemica virus in bank voles.three strains of nephropathia epidemica (ne) virus were isolated from lung tissues of bank voles (clethrionomys glareolus) and a grey-sided vole (c. rufocanus) trapped in västerbotten county, sweden. two of these isolates were serially passaged in seronegative laboratory-bred bank voles. experimentally infected animals developed a subclinical infection characterized by virus persistence, particularly in lung tissue. attempts to infect other species of colonized rodents with ne virus and to isola ...19846208601
an immune adherence assay for discrimination between etiologic agents of hemorrhagic fever with renal syndrome.antigens of the viruses that cause hemorrhagic fever with renal syndrome (hfrs) and antibodies to these antigens were titrated in an immune adherence hemagglutination (iaha) test and a cf test. vero-e6 cells infected with the sr-11 strain of hfrs virus, an isolate from rats associated with a laboratory outbreak in japan, and the 76-118 strain of hantaan virus, the etiologic agent of korean hemorrhagic fever, were used as antigens in the iaha, cf, and indirect immunofluorescent antibody (ifa) tes ...19846141206
antibodies to the hfrs virus in the human population of european rsfsr as detected by radioimmunoassay.natural immunity to the causative agent of hemorrhagic fever with renal syndrome (hfrs) has first been studied using radioimmunoassay (ria) in the human population of the bashkir assr with the highest incidence of this infection and of five other regions of the rsfsr with lower incidence of hfrs. the antigen was prepared as a suspension of lungs of rodent from natural hfrs foci and contained a high concentration of virus protein. 12,000 sera from the population of 6 areas of the rsfsr were exami ...19846141781
[survey on the reservior for hemorrhagic fever-renal syndrome in yingshang county, anhui province]. 19846146404
[hantavirus infections in the netherlands]. 19846441122
nephropathia epidemica in norway: antigen and antibodies in rodent reservoirs and antibodies in selected human populations.nephropathia epidemica (ne) antigen was detected by ifat (indirect fluorescent antibody technique) in the lungs of 14 of 97 bank voles (clethrionomys glareolus) collected in three endemic areas. the distribution of antigen positive voles within an endemic location was scattered. antibodies to korean hemorrhagic fever (khf) virus antigens were detected by ifat in 12 of 14 ne antigen positive bank voles and in 15 of 83 that were antigen negative. ne antigen positive voles exhibited higher antibody ...19846146649
experimental infection in newborn mice and rats by hemorrhagic fever with renal syndrome (hfrs) virus.newborn mice and rats were inoculated intracerebrally (ic) or intraperitoneally (ip) with hantaan virus (76-118 strain) or hfrs-related virus (b-1 strain). the mortality and the influence on the increase of body weight in newborn mice were higher in the groups infected with the 76-118 strain than in the groups infected with the b-1 strain, while the b-1 strain was more virulent in rats than the 76-118 strain. virus isolation from rats inoculated with either strain was attempted 7 and 11 weeks af ...19846442757
[prevalence of ectoparasites, endoparasites, bacteria and anti-hantaan virus antibodies in wild rats captured in harbor area of shimizu, japan]. 19846431033
two cases of hemorrhagic fever with renal syndrome in northern greece.two cases of hemorrhagic fever with renal syndrome were diagnosed in woodcutters presumably exposed to wild rodents and their urine in a forest in northern greece. the disease was characterized by acute renal insufficiency without hemorrhagic manifestations. one patient required hemodialysis, but both recovered without sequelae and developed a fourfold increase in titer of antibody to hantaan virus, as determined by an immunofluorescence test. these are the first reported cases of hemorrhagic fe ...19846145743
hantavirus disease. 19846146051
antigenic difference between viral strains causing classical and mild types of epidemic hemorrhagic fever with renal syndrome in china.the antigenic relationship between viral isolates from apodemus and rattus that appear to cause the classical and mild types of epidemic hemorrhagic fever (ehf) in china was studied by cross-immunofluorescence, cross-neutralization, immunofluorescence blocking tests, and cross-enzyme-linked immunosorbent assay (elisa). obvious antigenic diversity between the isolates was demonstrated by cross-neutralization, immunofluorescence blocking tests, and cross-elisa. antisera from patients with classica ...19846150062
hemagglutination-inhibiting antibody in hemorrhagic fever with renal syndrome.hemagglutination activity was demonstrated in a hantaan virus antigen prepared from infected suckling mouse brain by sucrose-acetone extraction. hemagglutination of goose erythrocytes was ph dependent and was optimal at ph 6.0-6.2. immunofluorescent and hemagglutination-inhibiting antibodies were significantly correlated in 80 human and 13 animal sera. hemagglutination-inhibiting antibody was present in some patients 20 years after the onset of hemorrhagic fever with renal syndrome. the hemagglu ...19846150063
[studies on growth conditions of ehf virus in primary rat lung cell culture]. 19846241087
[animal reservoirs of epidemic hemorrhagic fever--experimental studies and identification of the specificity of rat lung antigens. cooperative research group for epidemic hemorrhagic fever]. 19846242546
determination by immune adherence ha of the antigenic relationship between rattus- and apodemus-borne viruses causing hemorrhagic fever with renal syndrome. 19846201565
[the diagnosis of hemorrhagic fever with renal syndrome using an indirect immunoenzyme histochemical method with horseradish peroxidase-labelled staphylococcus protein a]. 19846152605
evaluation of focus reduction neutralization test with peroxidase-antiperoxidase staining technique for hemorrhagic fever with renal syndrome virus.titers of hemorrhagic fever with renal syndrome virus were estimated by counting foci stained with peroxidase-antiperoxidase or by using immunofluorescence methods. foci stained with pap were clearer and easier to count. the peroxidase-antiperoxidase method showed a linear relationship between virus concentration and the number of foci. a focus-reduction neutralizing antibody test was performed with immune rat sera and sera from patients. again, the peroxidase-antiperoxidase method proved to be ...19846440908
hantaan-like viruses from domestic rats captured in the united states.recent observations that rattus rodents in asia harbor hantaan (htn)-like viruses associated with hemorrhagic fever with renal syndrome led us to investigate the possibility that similar viruses might be present in the united states. wharf rats were captured at major port cities, their sera were examined for htn antibody by immunofluorescent antibody (ifa) assay and tissues from selected antibody-positive rats were examined for htn antigen. rats positive for both antibody and antigen to htn viru ...19846435464
[isolation of strains of the virus of hemorrhagic fever with renal syndrome in cell culture].four strains of hemorrhagic fever with renal syndrome (hfrs) virus were isolated from the lungs of cl. glareolus caught in natural foci of hfrs in the european ussr. preliminary experiments using enzyme-immunoassay (eia) established the appurtenance of hfrs antigen in lung suspensions to the "western" serotype. four strains were isolated in vero-e6 cell culture after several blind passages and identified as the "western" serotype of hfrs virus. one stram originally isolated in wistar rats and pa ...19846437079
isolation in vero-e6 cells of hanta virus from clethrionomys glareolus captured in the bashkiria area of the u.s.s.r. 19846442126
infectivity titration of hemorrhagic fever with renal syndrome virus: use of immune adherence hemagglutination for detection of virus growth.serial dilutions of hemorrhagic fever with renal syndrome viruses were inoculated into vero-e6 cells in microplates. after 2 weeks of incubation, infected cells were disrupted by freezing and thawing, and virus antigens were detected by immune adherence hemagglutination. the infectivity titers of the virus as determined by this method were in close agreement with those obtained by the immunofluorescent antigen endpoint method. then, a neutralization method was established. japanese hemorrhagic f ...19846436305
isolation of haemorrhagic fever with renal syndrome virus from leukocytes of rats and virus replication in cultures of rat and human macrophages.newborn rats were inoculated intraperitoneally with haemorrhagic fever with renal syndrome (hfrs)-related virus (b-1 strain), and virus isolation from their various organs was attempted between 1 and 25 weeks after inoculation. virus could be isolated repeatedly from lung, brain, spleen and kidney and also from peripheral blood. when virus isolation was carried out on fractionated peripheral blood cells, virus was associated mainly with the macrophage fraction and to a lesser extent with granulo ...19853925075
definition of three serotypes of hantaviruses by a double sandwich elisa with biotin-avidin amplification system.a total of seven different isolates of hantaviruses from five mammalian species and six geographical areas were examined by a double sandwich elisa with biotin-avidin amplification system. three serotypes were recognized by comparing antigen titres to homologous and heterologous antibody and blocking antibody titres to homologous and heterologous antigens. serotype 1 included four strains: hantaan virus isolated from apodemus agrarius in south korea, hupei-i isolated from acute-phase serum from ...19853926952
partial characterization of prospect hill virus isolated from meadow voles in the united states. 19853930622
certified cell lines of rat origin appear free of infection with hantavirus. 19853930626
serologic evidence of hantaan-like virus in rodents and man in thailand.a serologic survey was conducted to determine the presence of antibody to hantavirus in rodents in thailand. sera from over 300 rodents were tested by an immunofluorescent antibody method. bandicota indica, a field rodent, was found to have a high incidence of infection (20-24%) in 2 locations. a hantavirus was isolated from lung samples of b. indica. when sera were tested from humans living in kanchanaburi and several locations in bangkok, those people living in close proximity to the infected ...19853938073
reservoir animals for nephropathia epidemica in norway: indications of a major role for the bank vole (c. glareolus) in comparison with the woodmouse (a. sylvaticus).small rodents were collected live in two different locations within a nephropathia epidemica (ne) endemic area, and tested for both antiviral serum antibodies and viral antigens in lung sections. in one location, only apodemus sylvaticus (woodmice) were found in the traps, in the other, both a. sylvaticus and clethrionomys glareolus (bank voles) were collected. among the woodmice from the former location the prevalence of ne virus markers was significantly lower than for either woodmice or bank ...19853919085
a scottish case of nephropathy due to hantaan virus infection.an illness characterised by acute renal failure, fever, conjunctivitis and a macular erythematous centrifugal rash is described in a scot who had not recently travelled abroad. there was serological evidence that the illness was due to hantaan or a closely related virus.19852863308
efficacy, toxicology and clinical applications of ribavirin against virulent rna viral infections. 19852867737
experimental infection with puumala virus, the etiologic agent of nephropathia epidemica, in bank voles (clethrionomys glareolus).subclinical chronic infections characterized by transient viremia, prolonged virus shedding in oropharyngeal secretions and feces, and virus persistence in tissues (particularly lung) developed in laboratory-bred weanling bank voles (clethrionomys glareolus) inoculated intramuscularly with puumala virus (strain hällnäs), the etiologic agent of nephropathia epidemica. viral antigen, as evidence by granular fluorescence, was detected in the lungs, liver, spleen, pancreas, salivary glands, and smal ...19852861296
[the domestic cat as the host animal of the epidemic hemorrhagic fever virus]. 19852870786
[hantaan viruses. pathogenic agents in zoonoses]. 19852861673
[cases of hemorrhagic fever renal syndrome in 3 counties of the guang xi zhuang tribe autonomous region for the first time with the recovery of hemorrhagic fever viral agents from infected rodents]. 19852859926
differential susceptibility and resistance of immunocompetent and immunodeficient mice to fatal hantaan virus infection.susceptibility and resistance to fatal hantaan virus meningoencephalitis were studied in immunocompetent (nu/+) and congenitally t-cell deficient (nu/nu) cd-1 mice of different ages. susceptibility of nu/+ mice to fatal infection was age-dependent, as evidenced by 100 percent mortality in mice inoculated intracerebrally with hantaan virus (strain 76-118) during the first week of life, 60 percent mortality in mice inoculated at 9 days of age, and no disease or death in mice inoculated at 14 to 42 ...19852864037
antiviral neutralizing antibody to hantaan virus as determined by plaque reduction technique.the 76--118 strain of hantaan virus was titrated in e6 (vero) cells by the plaque method using agarose overlay medium. visible plaques, formed 10 days post-infection, were uniformly 2--3 mm in diameter. dose-response experiments showed that a single infectious particle initiated the formation of a plaque. infectivity titers by the plaque method were equivalent to those obtained by the endpoint method (tcid50) using the immunofluorescence antibody technique (ifa) for antigen detection. the single ...19852859845
[morphological study of two strains of hemorrhagic fever renal syndrome virus isolated from patients]. 19852861905
[hemorrhagic fever with renal syndrome in penza province].the evidence of the existence of natural foci of hfrs in penza region has first been presented on the basis of serological examinations of patients and normal population as well as hfrs virus antigen demonstration in the lung tissue of small mammals. an outbreak of hfrs among children in a pioneer camp is described. aerogenic infection of the children contracted from hfrs virus-infected bank voles (clethrionomys glareolus) living near the camp is assumed. the detection of hfrs antigen in 5 speci ...19852860753
outbreak of haemorrhagic fever with renal syndrome in greece.an outbreak of eight cases of haemorrhagic fever with renal syndrome in north-west greece is presented. the major clinical manifestation was fever and all patients subsequently developed decreased renal function and proteinuria. the disease was diagnosed by rising antibody titers to the hantaan virus.19852861091
serological and virological evidence of a hantaan virus-related enzootic in the united states.the first isolation of a hantaan-related virus from a feral rat in the united states was made from a rattus norvegicus caught in new orleans. the strain, designated tchoupitoulas virus, is antigenically related to, but distinct from, the prototype strain 76-118 of hantaan virus and is the first hantaan-like virus isolated from the pancreas of a naturally infected animal. serosurveys of wild rodents from urban and rural areas in the united states indicated that hantaan-related viruses infected ur ...19852861241
[detection of natural foci of hemorrhagic fever with renal syndrome on the territory of the komi assr].natural foci of hemorrhagic fever with the renal syndrome (hfrs) have been found for the first time in the komi assr. the main source of the causative agent in this territory was clethrionomys glareolus the average infection rate of which was 4.4% and in some areas as high as 22.6%. for the first time in the republic the portion of human population naturally immune to hfrs was determined, comprising 5.2% of the population. the immune structure of the population did not differ from that in other ...19852864760
hantaan virus infection in suckling mice: virologic and pathologic correlates.the hantaan virus suckling mouse model was examined to delineate virologic and histopathologic characteristics of infection at the organ level. viral antigen and infectious virus were detected in all organs examined, with highest titers achieved in brain, lung, and kidney. a constellation of histologic lesions was identified in brain (diffuse meningoencephalitis with bilaterally symmetrical thalamic necrosis), liver (pericholangiohepatitis), lung (pneumonitis), and spleen (lymphoid hyperplasia); ...19852865331
[characteristics of the epizootic process in hemorrhagic fever with renal syndrome].for the first time in the study of epizootology of hemorrhagic fever with the renal syndrome (hfrs) in wild animals data are presented indicating certain relationship between true infection rate of bank voles with hfrs virus and the content of the circulating antigen of hfrs virus and antibody to this virus in these animals. different age groups of animal populations were shown to have different intensity of immunity: it was more marked in animals of older age groups, and less in young animals. ...19852865854
[seroepidemiology of hemorrhagic fever with renal syndrome (hfrs) virus in fukuoka]. 19852866153
serotypic classification of hantaviruses by indirect immunofluorescent antibody and plaque reduction neutralization tests.antisera prepared against 16 strains of hantaviruses isolated from patients with hemorrhagic fever with renal syndrome (hfrs) or from rodents captured in hfrs-endemic and nonendemic regions were titrated against hantaan virus strain 76-118, puumala virus strain sotkamo, and prospect hill virus strain prospect hill-i by using the indirect immunofluorescent antibody and plaque reduction neutralization tests. isolates fell into one of four distinct groups or serotypes. serotype 1 included apodemus- ...19852866197
[hemorrhagic fever with renal syndrome caused by puumala virus. 2 cases with hypertriglyceridemia in the aube department]. 19852866508
pathogenesis of hantaan virus infection in suckling mice: clinical, virologic, and serologic observations.hemorrhagic fever with renal syndrome (hfrs) is a debilitating disease of humans caused by hantaan virus (hv), the prototype member of a newly proposed genus of bunyaviridae. studies of hv pathogenesis have been limited by the absence of a well defined model for a virus-induced disease state. in an attempt to devise a model for hv pathogenesis in laboratory rodents, newborn outbred suckling icr mice were shown to be uniformly susceptible to lethal infection with non-mouse adapted hv by intracere ...19852858987
serological evidence of hantaan virus infection in the united states.we found low titers of fluorescent antibodies against hantaan virus, the etiologic agent of korean hemorrhagic fever, in sera from 7 of 1,035 patients with febrile illnesses of unknown origin and from 6 of 664 blood donors in the united states. all but 1 of these individuals possessed neutralizing antibodies against hantaan virus. this was a 31-year-old research technician who had worked with laboratory rodents with virus-induced tumors, but had not traveled abroad, suggesting that infection wit ...19852858988
isolation of a hantaan-related virus from brazilian rats and serologic evidence of its widespread distribution in south america.a serosurvey of domestic rats was conducted in several south american cities between september 1982 and march 1983 for evidence of hantavirus infection. antibody-positive rats were found in belem, são paulo and recife-olinda, brazil and in buenos aires, argentina, with the highest antibody prevalence rate detected in belem (30 positive of 54 tested, 56%). a virus isolated from tissues of a rattus norvegicus captured in belem, was shown to be antigenically similar to girard point viruses isolated ...19852862803
[epidemiological study of epidemic hemorrhagic fever in china]. 19852862996
[main reservoirs of infection of the hemorrhagic fever renal syndrome in endemic areas of anhui province]. 19852862997
[relationship between hosts and hemorrhagic fever renal syndrome in chang-an county, shan-xi province]. 19852862998
isolation of haemorrhagic fever with renal syndrome virus from suncus murinus, an insectivore. 19852857871
hantaan virus. 19852861238
immune spleen cell-mediated protection against fatal hantaan virus infection in infant mice.to clarify the basis of the age-dependent susceptibility of infant mice to fatal meningoencephalitis caused by hantaan virus, we investigated the ability of spleen cells from immune and nonimmune weanling balb/c mice to confer protection to syngeneic infant mice. intraperitoneal transfer of 10 x 10(7), 5 x 10(7), 2.5 x 10(7), and 1.2 x 10(7) immune spleen cells to infant mice 24 hr after intracerebral challenge with 100 50% lethal doses of hantaan virus (strain 76-118) resulted in 100%, 70%, 64% ...19852857753
antigenic and genetic properties of viruses linked to hemorrhagic fever with renal syndrome.hemorrhagic fever with renal syndrome (hfrs) comprises a variety of clinically similar diseases of viral etiology that are endemic to and sporadically epidemic throughout the eurasian continent and japan. although hfrs has not been reported in north america, viruses that are antigenically similar to hfrs agents were recently isolated from rodents in the united states. examination and comparison of eight representative isolates from endemic disease areas and from regions with no known associated ...19852858126
epidemiological study of hemorrhagic fever with renal syndrome related virus infection among urban rats in two islands in tokyo bay, japan.a seroepidemiological survey of hemorrhagic fever with renal syndrome (hfrs) virus infection in 403 urban rats (rattus norvegicus) captured on two different islands in tokyo bay was carried out using the indirect immunofluorescent antibody (ifa) test with the sr-11 strain of hfrs virus. antibody positive sera were detected in 99 out of 355 (27.9%) rats from the island with reclaimed ground area, but none were detected in 48 rats from the other islands. ifa titers of the positive sera varied from ...19852859764
distinction between bunyaviridae genera by surface structure and comparison with hantaan virus using negative stain electron microscopy.ultrastructural studies of glutaraldehyde-fixed viruses of the bunyaviridae were performed by negative-stain electron microscopy. the surface structure of viruses of each genus was compared with that of the other genera and with hantaan virus, the prototype of a proposed new genus of bunyaviridae. viruses of each genus had a surface structure distinct for that genus. in addition, hantaan virus had a surface structure composed of a grid-like pattern of morphologic subunits not previously describe ...19852412528
[reservoirs of the causative agent of hemorrhagic fever with renal syndrome in maritime territory foci].examinations of 6362 small mammals of 18 species revealed the main reservoirs of the causative agent of hemorrhagic fever with the renal syndrome (hfrs) in foci of the primorsky krai, and demonstrated the importance of individual species in the epidemiology of the infection. data on the circulation in the region of antigenically different serotypes of hfrs virus are presented.19852408384
new isolates of hfrs virus in sichuan, china and characterisation of antigenic differences by monoclonal antibodies. 19862423827
characterization of glycoproteins of viruses causing hemorrhagic fever with renal syndrome (hfrs) using monoclonal antibodies.viruses causing hemorrhagic fever with renal syndrome (hfrs) encode two glycoproteins, g1 and g2. for determination of the biological functions of these glycoproteins, we isolated 15 hybridomas secreting monoclonal antibodies directed against the glycoproteins of the b-1 and hantaan viruses (hv). from results of neutralizing and hemagglutination inhibition (hi) tests, and studies on the antigenic reactivities of the antibodies with other hv-related viruses by immunofluorescence, we classified th ...19862422813
[isolation of strains of the virus of hemorrhagic fever with renal syndrome in a cell culture from human patients and rodents].the virus of hemorrhagic fever with renal syndrome serologically similar to hantaan virus was isolated from three rodent species, ap. peninsulae, cl. rutilus, cl. rufocanus, and from human patients in vero e6 cell culture.19862425494
serologic survey of selected pathogens in white-tailed and mule deer in western nebraska.exposure of free-ranging white-tailed deer (odocoileus virginianus) and mule deer (odocoileus hemionus) in western nebraska to selected livestock pathogens was determined by serology and attempted virus isolation. antibodies to bluetongue virus, epizootic hemorrhagic disease virus, and bovine respiratory syncytial virus were present in both species of deer. no serologic reactors to brucella or anaplasma were found. attempts to isolate bluetongue virus were negative.19862845156
persistent infection of rats with haemorrhagic fever with renal syndrome virus and their antibody responses.newborn (within 24 h after birth), 1-week-old and 6-week-old (adult) rats were inoculated with a hantaan-related virus (b-1) and attempts were made to isolate the virus from various organs. virus-specific antigens were detected in various organs of newborn rats. moreover virus could be isolated from almost all their organs even 25 weeks after infection. in contrast, in rats infected at 6 weeks of age the virus could be isolated from various organs but its concentration decreased progressively wi ...19862878971
comparison of immunofluorescence and hemagglutination inhibition tests and enzyme-linked immunosorbent assay for detection of serum antibody in rats infected with hemorrhagic fever with renal syndrome virus.serum antibodies against the b-1 strain of hemorrhagic fever with renal syndrome (hfrs) virus in wild and experimental rats were investigated by the indirect immunofluorescent antibody (if) test, the hemagglutination inhibition (hi) test, and an enzyme-linked immunosorbent assay (elisa). in the newly developed elisa, monoclonal antibodies to the b-1 strain were applied as coating antibody. in sera from wild rats, the results obtained by the elisa agreed quite well with those obtained by the hi t ...19862877007
[hemorrhagic fever with renal syndrome in madagascar. first seroepidemiologic survey of rat populations]. 19862879517
[serological data on hemorrhagic fever with renal syndrome in southeast asia].using an indirect immunofluorescent test with hantaan 76-118 and puumala strains as antigens, antibodies against haemorrhagic fever with renal syndrome viruses were found for the first time in human in laos and viet-nam and confirmed the previously published results in thailand. these results suggest the existence of an hantaan-related virus in these countries.19862879646
infection of laboratory workers with hantavirus acquired from immunocytomas propagated in laboratory rats.hantavirus has been isolated in cell culture from rat immunocytomas used and stored at a research laboratory in the u.k. where there was evidence of a laboratory-acquired infection leading to haemorrhagic fever with renal syndrome. both transplantation into lou/m/wsl rats and storage of passaged immunocytomas at -70 degrees c over a period of 8-10 years had not eliminated the virus. the isolates were identified as hantavirus by means of serum obtained from patients with hantavirus infection as w ...19862871112
detection of hemagglutination-inhibiting antibodies in patients with nephropathia epidemica and korean hemorrhagic fever by using puumala virus cell culture antigen. 19862871114
ribavirin therapy for hantaan virus infection in suckling mice.we recently reported that ribavirin inhibited hantaan virus (hv) replication in vitro. in the present study, we used the hv suckling mouse model to evaluate the efficacy of treatment with various doses of ribavirin. beginning on day 10, untreated animals, infected with ten times the amount of hv (strain 76/118) required to kill 50% of the animals, lost weight; by days 15 to 18, they developed paralysis of both hind limbs, and they died between days 20 and 21. treatment with 50 mg of ribavirin/kg ...19862869089
growth experiment of hantaan virus in a549 cells: an attempt to improve the immunofluorescent antibody technique for hemorrhagic fever with renal syndrome.an assay method for the infectivity of hantaan virus, a causative agent of hfrs (hemorrhagic fever with renal syndrome), was developed by the use of ifa (immunofluorescent antibody technique). with the aid of this method, the growth characteristics of hantaan virus, 76-118 strain, were followed in a549 cells. at a maximal moi (multiplicity of infection) of 1.6 vaiu (viral antigen-inducing units) per cell, the conventionally available value, plateau level potencies of the viral antigen and virus ...19862872077
[epidemiologic study of epidemic hemorrhagic fever in guangzhou]. 19862872966
[hemorrhagic fever with renal syndrome among human populations of tiantai county]. 19862872967
serological survey with the antigen of haemorrhagic fever virus with renal syndrome in small rodents in slovakia.results of serological survey in small rodents with the antigen of haemorrhagic fever with renal syndrome (hfrs) virus indicate the existence of a natural focus of this virus in slovakia. antibodies were found in the following free-living rodent species: clethrionomys glareolus, apodemus agrarius, apodemus sylvaticus, pitymys subterraneus, microtus arvalis and microtus economus. a total 10 out of 120 (8.2%) small rodents trapped in investigated localities of western and eastern slovakia had anti ...19862873734
pathogenesis of experimental hantaan virus infection in laboratory rats.weanling fischer rats inoculated intramuscularly with hantaan virus (strain 76-118) developed subclinical infections characterized by transient viremia and shedding of virus in saliva, persistence of virus in lung, pancreas, spleen and liver, and development of fluorescent and neutralizing antibodies in serum with immune complex deposition in lung. viremia and virus shedding in saliva occurred 10 to 13 days after inoculation. horizontal intracage transmission of infection occurred between 35 and ...19862869749
epidemiological investigations following an outbreak of hemorrhagic fever with renal syndrome in greece.epidemiological investigations were conducted following an outbreak of hemorrhagic fever with renal syndrome (hfrs) which occurred in the state of epirus, northwestern greece, in july and august 1983. a total of 8 patients were hospitalized during the outbreak; 3 were severely ill and 1 died. a serosurvey made in may 1984 sampled 184 of the approximately 400 residents of the village of tsepelovo, where 4 patients resided, and found 12 (6.5%) persons, including convalescent sera from 4 patients, ...19862871769
haemagglutination-inhibition test for haemorrhagic fever with renal syndrome using virus antigen prepared from infected tissue culture fluid.haemagglutinating (ha) antigens of four strains of virus related to that causing haemorrhagic fever with renal syndrome (hfrs) were prepared from infected tissue culture fluids by ultracentrifugation. the titres of the precipitated antigens were increased considerably by acetone extraction and sonication. acetone extraction completely inactivated infectious virus in the antigen preparations. the antigens were ph-dependent, with ph optima at 5.8. good correlations were observed in human and rat s ...19862868067
hantavirus nephropathy in western europe: ubiquity of hemorrhagic fevers with renal syndrome. 19862869650
[hemorrhagic fever with a renal syndrome]. 19862882632
the antigenic analysis of haemorrhagic fever with renal syndrome viruses in china by monoclonal antibodies.thirty-six strains of haemorrhagic fever with renal syndrome (hfrs) virus were isolated from patients and a number of host animals in various areas in china. they were analysed by an immunofluorescence test (ifat) using 10 monoclonal antibodies (mcabs) specific for the hfrs virus; antigenic differences among the strains have been demonstrated. the hfrs virus strains revealed nine different reactions with the mcabs, showing that there are at least nine different antigenic determinants including g ...19862878042
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