PMID(sorted ascending)
localization and changes of hemorrhagic fever with renal syndrome virus in lymphocyte subpopulation.double-labeled immunocytochemistry was used for studying the localization and distribution of hemorrhagic fever with renal syndrome virus (hfrsv) antigen in the peripheral blood lymphocyte subpopulations in hfrs patients and the quantitative changes of these subpopulations. it was found that hfrsv might infect different subpopulations of lymphocytes. the infection rate of b cells (31.3%) was significantly higher than that of t cells (8.5%), and the infection rate of cd8+ subpopulation (10.5%) wa ...19911680612
acute hantavirus infection. 19911681336
nephropathia epidemica (hemorrhagic fever with renal syndrome) in scandinavia.nephropathia epidemica (ne) in scandinavia is a zoonosis caused by puumala virus. the main animal reservoir is the bank vole. ne predominantly affects men. its annual incidence varies in a cyclic fashion, with peaks occurring every third to fourth year. the clinical picture of ne in scandinavia is similar to that of hemorrhagic fever with renal syndrome in other parts of the world, although ne generally has a milder course. the case-fatality rate is approximately 0.2%. the most common clinical f ...19911681581
hantaan antibody in italian ex-soldiers who served in the balkans. 19911682675
rapid diagnosis of hantavirus disease with an igg-avidity assay.nephropathia epidemica (ne), due to puumala virus, is endemic in eastern europe and scandinavia. serodiagnosis of ne relies on conventional indirect immunofluorescence to detect igg against puumala virus. however, in conventional serology, most patients with acute ne have raised but stable non-diagnostic antibody titres. for better serodiagnosis of ne, we have developed a test that measures the avidity (functional affinity) of igg antibodies against puumala virus. this new assay was highly speci ...19911682736
[hemorrhagic fever with renal syndrome in the paris region]. 19911682914
[endemic occurrence of hemorrhagic fever with renal syndrome in the federal republic of germany]. 19911683080
susceptibility of laboratory and wild rodents to rattus or apodemus-type wistar rats (rattus norvegicus), apodemus agrarius, meriones unguiculatus, clethrionomys rufocanus, and apodemus argenteus were inoculated with rattus-type (sr-11, ki-262, and tb-314) or apodemus-type (hantaan 76-118) hantaviruses. production of serum antibody to the inoculated virus (iaha titres of 1:32 to 1:4 096) was obsersved in all rodent species 10 weeks after virus inoculation. rattus-type virus was detected in some organs of all the rodent species employed except of apodemus agrari ...19911683116
arthropod vectors in the evolution of bunyaviruses.viruses from each genus of bunyaviridae have preferential relationships to the arthropods of only one or two families, i.e. bunyavirus to mosquitoes (culicidae), phlebovirus to sand flies (psychodidae) and mosquitoes, uukuvirus and nairovirus to ticks (ixodidae and argasidae). an exception is genus hantavirus not proven to be transmitted by vectors. within the bunyavirus genus 16 serogroups have been recognized on the basis of their antigenic relationship. based on isolations from the nature eac ...19911683124
hantaviral antigens and antibodies in wild rodents in portugal.small rodents of the species rattus norvegicus and rattus rattus have been captured between 1986 and 1988 in several areas of southern portugal. a total of 135 animal specimens were examined for hantaviral antigens in lung sections and 5 have been found positive. some of the rodents were shown to have serum antibodies as detected by immunofluorescence in titres up to 1:256. this investigation proves for the first time the presence of hantavirus in wild rodent populations of portugal.19911683135
prospective, double-blind, concurrent, placebo-controlled clinical trial of intravenous ribavirin therapy of hemorrhagic fever with renal syndrome.a prospective, randomized, double-blind, concurrent, placebo-controlled clinical trial of intravenous ribavirin (loading dose of 33 mg/kg, 16 mg/kg every 6 h for 4 days, and 8 mg/kg every 8 h for 3 days) was conducted in 242 patients with serologically confirmed hemorrhagic fever with renal syndrome (hfrs) in the people's republic of china. mortality was significantly reduced (sevenfold decrease in risk) among ribavirin-treated patients, when comparisons were adjusted for baseline risk estimator ...19911683355
a swedish fatal case of nephropathia epidemica.a previously healthy 55-year-old man with nephropathia epidemica (ne) developed disseminated intravascular coagulation, anuria and shock and died on day 6 of his disease. by use of indirect immunofluorescence technique and elisa, specific serum igm antibodies against puumala virus could be detected, thus confirming the clinical diagnosis. this case demonstrates that ne in scandinavia is a potentially lethal disease.19911683493
further uk case of acute hantavirus infection. 19911683956
[experimental studies on the transmission of hemorrhagic fever with renal syndrome virus by gamasidea mites and fleas].hemorrhagic fever with renal syndrome (hfrs) virus was isolated from 3 species of gamasidea mites (haemolaelaps glasgowi, laelaps jettmari, eulaelaps shanghaiensis), and 2 species of fleas (monopsyllus anisus, leptopsylla segnis) collected from nests of hfrs virus-infected apodemus agrarius, whereas hfrs virus was not recovered from gamasidea mites and fleas collected from nests of uninfected apodemus agrarius. it was thus confirmed that hfrs is a disease of animal origin with natural foci of in ...19911684135
[acute kidney failure caused by hantavirus infection].a 37-year-old man suddenly fell ill with high fever (up to 39.6 degrees c), headache and lumbar pain. there was marked thrombocytopenia (minimal level of 48,000/microliters), moderate anaemia and a slow rise in serum lipase concentration to maximally 1352 u/l. marked sinus bradycardia (to as low as 34 beats/min) occurred in the further course of the disease, as well as upper abdominal pain (endoscopically diagnosed as antral gastritis), subileus and splenomegaly. two haemodialysis treatments wer ...19911684152
antibodies to bluetongue and epizootic hemorrhagic disease viruses in a barrier island white-tailed deer population.from 1981 through 1989, serum samples from 855 white-tailed deer (odocoileus virginianus) from ossabaw island, georgia (usa), were tested for antibodies to bluetongue virus (btv) and epizootic hemorrhagic disease virus (ehdv). during this period, prevalence of precipitating antibodies to btv and ehdv as determined by agar gel immunodiffusion (agid) tests decreased from 74% to 3% and from 34% to 1%, respectively. antibodies were detected in serum samples from 0.5-yr-old deer only during 1981, 198 ...19911684621
comparison of european isolates of viruses causing hemorrhagic fever with renal syndrome by a neutralization test.different virus isolates causing hemorrhagic fever with renal syndrome (hfrs) were compared using a neutralization test. patient convalescent sera and antisera prepared in rabbits were used to compare puumala-related hantavirus isolates from finland, sweden, belgium, and the ussr. the majority of european isolates were indistinguishable from each other using both homologous rabbit antisera and patient convalescent sera. the european isolates of hfrs were also compared with prototype hantaan (the ...19911684888
[hantavirus nephritis: endemic in the reutlingen area]. 19911685046
etiology and epidemiology of hemorrhagic fever with renal syndrome. 19911685201
mechanisms of disease in hemorrhagic fever with renal syndrome.hemorrhagic fever with renal syndrome (hfrs) is a dramatic illness characterized by fever, and variable degrees of circulatory failure, hemorrhage, and renal insufficiency. hantaviruses are the cause of this syndrome. in its severe form, it is associated with significant mortality. vascular dysfunction is the key physiologic derangement in this disorder. there is a growing body of evidence that suggests that immune mechanisms account for this derangement.19911685203
distribution of hantavirus serotypes hantaan and seoul causing hemorrhagic fever with renal syndrome and identification by hemagglutination inhibition epidemiologic evaluation of patients with hemorrhagic fever with renal syndrome from different locations in the people's republic of china was conducted to define the prevalence of two hantavirus serotypes, seoul (seo) and hantaan (htn). serum specimens were collected between 5 and 14 days after the onset of illness and were tested for antibodies by both hemagglutination inhibition (hi) and plaque reduction neutralization (prn). by the hi test, the geometric mean titer (gmt) of antibodies to ...19911685497
renal lesions in rats infected with rattus serotype hantavirus (sr-11 strain).hemorrhagic fever with renal syndrome (hfrs) virus, strain sr-11 (sr) was inoculated intraperitoneally into specific-pathogen-free (spf) newborn rats, from which the kidney lesions were examined pathologically. the infected rats revealed proteinuria on and after 16 days postinoculation (pi). histologically, the epithelial cells of the renal tubules showed mild vacuolar and granular degeneration with cytoplasmic inclusion bodies (cib) on and after 16 days pi. ultrastructurally, a decrease in numb ...19911686343
pathological studies on central nervous tissues of rats infected with rattus serotype hantavirus (sr-11 strain).newborn rats inoculated intraperitoneally with a hantavirus strain (sr-11) developed neurological signs such as ataxia and limb paralysis. the main histological lesions were scattered and multiple neuronal degeneration and necrosis with eosinophilic cytoplasmic inclusion bodies (cib) in the brain, spinal cord and ganglia. immunohistochemically, the viral antigen was detected in neurons, capillary endothelial and glial cells throughout the nervous tissue and cib were identified as consisting of h ...19911686344
effects of hantaan virus on human endothelial cells and their significance in pathogenesis of hemorrhagic fever with renal syndrome.the effects of hantaan virus (htnv) on human endothelial cells (hecs) were investigated both in vivo and in vitro. the 76-118 strain or sr-11 strain of htnv were inoculated into hecs monolayers respectively, and the virus antigens could be detected on the seventh day of the first passage after inoculation by immunofluorescent technique. the htnv could also be isolated through cultures of vero e-6 cells. htnv particles and inclusion bodies together with various changes in the organelles were obse ...19911686857
[the mechanisms of the development of tissue hypoxia and the criteria for its assessment in patients with hemorrhagic fever with renal syndrome].comparison of the parameters that characterize the activity of the sympathoadrenal system (urine adrenalin and noradrenalin) and the severity of hypoxia (lactic acid in blood and urine, pyruvic acid in blood and urine, lactate/pyruvate) in patients suffering from hemorrhagic fever with the renal syndrome (hfrs) showed them to be significantly and directly correlated. the time-course of changes in the study parameters mirrors the gravity of the pathological process. the authors hold that the symp ...19911687342
[cohort study of seroepidemiology on hemorrhagic fever with renal syndrome].a cohort study of seroepidemiology on hemorrhagic fever with renal syndrome (hfrs) was carried out in two villages, jiande county, zhejiang province. 1325 persons who had not had antibodies to hfrs in 1987 were interviewed and re-examined in 1988. seroconversion to hfrs antibodies was found in 30 persons (2.26%). among them, were two clinical cases with an incidence rate of 0.151% and the rate of inapparent infection was 2.12%. ratio of apparent to inapparent infection was 1:14. no infection was ...19911687775
[hemorrhagic fever with renal syndrome in croatia: incidence of infection in human and wild animal reservoirs].on the territory of lika and gorski kotar a group of 300 forest workers and 260 persons from the general population were screened for hantavirus antibodies (by the indirect immunofluorescence test). in the first group of examenees a mean of 1.6% were found positive, ranging from 0% (gospić) to 3.2% (ogulin). in the second groups a mean of 5.4% were positive, ranging from 4.4 to 8.9% (delnice and slunj, respectively). it is concluded that the hantavirus infection rate in our natural foci of hfrs ...19911688294
[the use of the indirect fluorescent antibody technic for determining viral antigens in the urine of patients with hemorrhagic fever with renal syndrome].the authors suggest a new technique for the detection of viral antigen in the urine of hfrs patients, based on the method for the differential cytologic rapid diagnosis of acute respiratory viral diseases. hfrs virus antigen was detectable in the urine sediment epithelium in 7 of the 8 patients from the seventh to the thirty-second day of the follow-up. fluorescent bright-emerald dots could be seen in the urine sediment cells, situated on the cell surface, in the cytoplasm round the nucleus and ...19901695982
[the nature of the persistence of the causative agent of hemorrhagic fever with renal syndrome in the body of its natural host and its relationship to the epizootiologic and epidemic processes]. 19901699361
detection of hantavirus rna in tissues of experimentally infected mice using reverse transcriptase-directed polymerase chain reaction.detection of hantaviruses, the etiological agents of hemorrhagic fever with renal syndrome (hfrs), by virus isolation using experimental animals or cell culture is time-consuming. a more rapid but equally specific method is needed. we used a reverse transcriptase-directed polymerase chain reaction (rt-pcr) to detect hantavirus genomic sequences and compared its sensitivity with conventional virus isolation. rna, extracted by the guanidinium isothiocyanate-cesium chloride method from hantavirus-i ...19911713266
antigenicity of hantavirus nucleocapsid proteins expressed in e. coli.dna clones representing the small genomic segment of nephropathia epidemica virus strain hällnäs b1 (nev) and hantaan virus strain 76-118 (htv) encoding their nucleocapsid proteins were inserted into the e. coli vector pin-iii-ompa for secretion of proteins into the periplasmic space. the complete htv and nev nucleocapsid proteins and two truncated versions of the nev nucleocapsid proteins were expressed as fusion proteins. unexpectedly, all products accumulated as insoluble aggregates. most of ...19911714110
antigenic variation of european haemorrhagic fever with renal syndrome virus strains characterized using bank vole monoclonal antibodies.monoclonal antibodies (mabs) against puumala (puu) virus, the aetiological agent of nephropathia epidemica, were produced by fusing activated spleen cells from a bank vole (clethrionomys glareolus) with the mouse myeloma cell line sp2/0. this novel approach, utilizing the natural vector of puu virus for hybridoma production, proved to be highly efficient, and eight stable puu virus-specific heterohybridomas were isolated and characterized. the bank vole mabs were all specific for the nucleocapsi ...19911716651
antigenic variance. 19911718665
coexpression of the membrane glycoproteins g1 and g2 of hantaan virus is required for targeting to the golgi study the intracellular transport and targeting to the golgi complex of the membrane glycoproteins g1 and g2 of hantaan virus, we have expressed them together and separately using recombinant vaccinia viruses. when expressed from the same recombinant vaccinia virus, g1 and g2 were localized to the golgi complex as analyzed by both immunofluorescence and subcellular fractionation. however, when the glycoproteins were expressed from separate recombinant viruses, both proteins remained in the en ...19921727611
[acute kidney failure and transient myopia. association suggestive of "hemorrhagic fever with renal syndrome"]. 19911828573
molecular characterization of the prospect hill virus m rna segment: a comparison with the m rna segments of other hantaviruses.complementary dna representing the genomic m rna segment of the prospect hill (ph) hantavirus was cloned and its nucleotide sequence determined. the ph virus m rna segment is 3707 nucleotides in length and has a long open reading frame in the viral complementary-sense rna with a coding capacity of 1142 amino acids. the predicted gene product of the ph virus m segment was compared with the corresponding gene products of hantaan virus strain 76-118 (hantaan), sapporo rat virus strain sr-11 (sr) an ...19911840609
nucleotide sequence and coding capacity of the large (l) genomic rna segment of seoul 80-39 virus, a member of the hantavirus genus.the nucleotide sequence of the large (l) genomic rna segment of seoul 80-39 virus was determined from overlapping cdna clones. the virion l rna segment is 6530 nucleotides long. the 3' and 5' terminal sequences are inversely complementary for 15 bases. the viral complementary-sense rna contains a single open reading frame from an aug codon at nucleotide position 37-39 to a uaa stop codon at nucleotide position 6490-6492. this orf could encode a polypeptide of 2151 amino acids (246,662 kda) which ...19911840713
experimental labyrinthitis in guinea pigs caused by a hantavirus.hantavirus-induced acute viral infection of the inner ear of guinea pigs is reported. guinea pigs were inoculated directly into the inner ear through the round window, or intraperitoneally with hantavirus (b-1 strain), and the cochlea was examined using an immunohistochemical technique for the localization of viral antigens. virus-specific antigens were detected prominently in the stria vascularis and to a lesser degree in other inner ear structures of the infected guinea pigs. the morphological ...19911901140
identification of hantavirus serotypes by testing of post-infection sera in immunofluorescence and enzyme-linked immunosorbent assays.serum samples were collected from 27 individuals who had been infected with a member of the genus hantavirus in the netherlands or belgium during the last 15 years. these samples were tested in an immunofluorescence assay (ifa) and two enzyme-linked immunosorbent assay (elisa) systems, using different virus strains that represented each of the four recently proposed serotypes of this genus. the serum samples from 11 individuals who had been infected through contacts with laboratory rats showed t ...19911901907
identification and characterization of a hantavirus strain of unknown origin by nucleotide sequence analysis of the cdna derived from the viral s rna segment.the genetic characterization of a serologically hantaan-like virus but of unknown origin (termed dx) was carried out by molecular cloning and nucleotide sequencing of the corresponding cdna of the viral s rna segment. the s rna was found to be 1765 nucleotides long with 3' and 5' termini being complementary for 24 bases. the virus messenger-sense rna contains one major open reading frame (orf) encoding 428 amino acids or a 50 kd polypeptide. a comparison of the dx s rna segment to those of sappo ...19911905433
stimulation of adherent cells by addition of purified proteins of viral hemorrhagic septicemia virus to trout kidney cell cultures.purified proteins of the virus causing viral hemorrhagic septicemia in the trout were added to cultures on semisolid medium of leukocytes obtained from either healthy or immunized rainbow trout. adherent cells were specifically stimulated by the glycoprotein of the viral spikes and, to a lesser extent, by the nucleoproteins. in contrast, a specific memory response was associated more with the nucleoproteins than with the glycoprotein when leukocytes from trout immunized with the virus were emplo ...19911905934
monoclonal antibodies to three strains of hantaviruses: hantaan, r22, and puumala.thirty hybrid cell lines that produce monoclonal antibodies to three strains of hantaviruses have been generated and characterized. one clone specific to hantaan 76-118 strain, four clones specific to rattus strains and one clone specific to puumala virus have been identified. most of the monoclones produced antibodies specific to nucleoproteins. only two monoclones were found to produce glycoprotein specific, neutralizing antibodies. the immunofluorescent (ifa) staining patterns of the monoclon ...19911907448
bunyaviridae: pathogenesis. 19911935228
bunyaviridae--natural history.obviously, the family bunyaviridae is comprised of a large number of epidemiologically diverse viruses. they vary dramatically in their vector and vertebrate host relationships, geographic distributions, and epidemic potential in humans and animals. public health practitioners, veterinarians, virologists, entomologists, biologists, ecologists, molecular biologists, and other scientists will all benefit from increased study and knowledge of this fascinating group of viruses.19911935229
hantaviruses in small wild living mammals in czechoslovakia. results of a 1983-1989 study.during a 7- year period were hunted small wild living mammals and examined by elisa and ria techniques for the presence of hantavirus antigen and/or antibodies by mfa. in total 3,050 animals of 16 species caught in 9 out of 10 regions of czechoslovakia, were examined. the proportion of positive animals was 4.4%. to the positive ones with the serotype 2 (western type) belonged the following: m. arvalis, c. glareolus, p. subterraneus, a. sylvaticus, a. flavicollis. to the eastern serotype: a. agra ...19911960391
molecular characterization and expression of glycoprotein gene of hantavirus r22 strain isolated from rattus norvegicus in china.a cdna containing the complete open reading frame of the m genome segment of hantavirus r22 strain isolated from rattus norvegicus in china, was amplified by polymerase chain reaction (pcr), and then cloned. the m segment is 3656 nucleotides in length with a predicted region of 3402 bases encoding a precursor glycoprotein of 1134 amino acids subsequently processed into viral glycoproteins 1 and 2 (g1 and g2). a strain comparison between r22 and sr11 (isolated from a rat in japan), and hantaan 76 ...19911962503
[nephropathia epidemica. hantavirus and acute renal insufficiency].puumala virus belongs to the hantavirus group and as other members of the group it can cause acute renal insufficiency. other hantaviruses are responsible for korean hemorrhagic fever and other hemorrhagic fevers with renal involvement. in scandinavia, hantavirus nephropathy is better known as nephropathia epidemica. hantavirus cause asymptomatic infections in mice and rats and the virus is thought to be transmitted to man via inhalation of desicated saliva, urine or feces from infected animals. ...19901967859
[hemorrhagic fever with renal syndrome]. 19901969359
[viral antigen localization and morphologic observation of the blood vessels in the skin of patients with hemorrhagic fever with early stage renal syndrome]. 19901969765
abnormalities of t cell immunoregulation in hemorrhagic fever with renal syndrome.the functions of spontaneous suppressor t cells and t lymphocyte subsets in patients with hemorrhagic fever with renal syndrome were compared. in the early stages of disease, decreased activity of spontaneous suppressor t cells was concurrent with increased numbers of cd8+ cells and a reversed cd4:cd8 ratio. these changes were related to abnormalities in serum c3 level and circulating immune complexes. in the recovery stages of the illness, spontaneous suppressor t cell activity and t cell subse ...19901969885
coding properties of the s and the m genome segments of sapporo rat virus: comparison to other causative agents of hemorrhagic fever with renal syndrome.three serologically distinct groups of hantaviruses have been associated with severe, moderate, and mild forms of hemorrhagic fever with renal syndrome (hfrs). to gain a better understanding of the genetic variation among these viruses, we cloned and sequenced the m and the s genome segments of sapporo rat virus, an etiologic agent of moderate hfrs, and compared the predicted gene products to those of hantaan virus, and the hällnäs strain of puumala virus, which are etiologic agents of severe an ...19901970443
infection with the puumala virus in pregnancy. case report. 19901970741
[electrolyte excretion in patients with a history of hemorrhagic fever with renal syndrome].renal transportation of sodium and potassium was studied in 85 patients who sustained hemorrhagic fever complicated with renal syndrome (hfrs). the examinees were given routine diet no. 7 recommended by pevzner. the conclusions were made on the analysis of circadian excretion and clearance of electrolytes studied and the levels of their water-load-induced tubular transportation: distal and proximal reabsorption and distal excretion of sodium, the correlation between potassium secretion and reabs ...19901970913
[the effect of hemodialysis on the vasopressin level and on the renin-aldosterone system in patients with hemorrhagic fever with renal syndrome].a total of 29 patients with severe hemorrhagic fever associated with the renal syndrome were studied for impact of extracorporeal hemodialysis on the activity of the renin-aldosterone system (ras), plasma vasopressin and osmolality and the levels of the major osmotically active agents, as well as the circadian urine output and blood pressures. in patients with oliguria there was a significant activation of ras, an increase in plasma vasopressin ad osmolality due to the increment of the urea in p ...19901970914
[hantaan virus infection--also in germany a cause of acute renal failure]. 19901971417
hemorrhagic fever with renal syndrome: mr imaging of the kidney.magnetic resonance (mr) imaging of the kidneys was performed in 14 patients with a severe form of hemorrhagic fever with renal syndrome (hfrs), a major public health problem in far eastern asia. to the authors' knowledge, this is the first report of renal assessment with mr imaging in hfrs. three mr imaging studies were done while patients were in the oliguric phase, 19 were done during the diuretic phase, and three were done during the convalescent phase. the authors differentiated the cortex a ...19901971450
hemorrhagic fever with renal syndrome. separation of human peripheral blood t and b cells and detection of viral this study, total blood lymphocytes were prepared from patients with hemorrhagic fever with renal syndrome (hfrs) by a density gradient on ficoll-hypaque. t and b cells were then purified by passing the total lymphocytes over a nylon wool column. the purities of total lymphocytes, t cells and b cells were 97.8 +/- 2.3%, 91.6 +/- 4.5% and 74.2 +/- 12.1%, respectively. also, after modification of cell fixation and smear drying, the number of cells were increased and the time needed for slide pr ...19901972048
antigenic subunits of hantaan virus expressed by baculovirus and vaccinia virus recombinants.baculovirus and vaccinia virus vectors were used to express the small (s) and medium (m) genome segments of hantaan virus. expression of the complete s or m segments yielded proteins electrophoretically indistinguishable from hantaan virus nucleocapsid protein or envelope glycoproteins (g1 and g2), and expression of portions of the m segment, encoding either g1 or g2 alone, similarly yielded proteins which closely resembled authentic hantaan virus proteins. the expressed envelope proteins retain ...19901972201
hemorrhagic fever with renal syndrome in a canadian serviceman. 19901972650
hantavirus infection in the united states: epizootiology and epidemiology.multiple species of murid and arvicolid (microtine) rodents serve as the reservoir hosts of hantaviruses, the etiologic agents of hemorrhagic fever with renal syndrome. three antigenically distinct hantaviruses have been isolated from rattus norvegicus, mus musculus, and microtus pennsylvanicus captured in the united states, and serologic evidence of a hantavirus enzootic has been found in several other indigenous rodent species. in residential districts of port cities such as baltimore, nearly ...19901972804
[characteristics of endemic foci of hemorrhagic fever with renal syndrome in various regions of the ussr].clinical, epizootological, and laboratory data for 9 endemic foci of hemorrhagic fever with renal syndrome (hfrs) were compared. the analysis covered 5120 cases of hfrs. it was established that in the foci, where the leading role is played by field mouse and japanese field mouse, hfrs is caused by the 1st serotype of hfrs virus, and the clinical course of the disease is quite severe. in the natural foci with the predominance of redbacked vole, the 2nd serotype of the virus circulates and the dis ...19901973011
[hemorrhagic fever with renal syndrome in lithuania]. 19901973012
[clinico-immunological characteristics of hemorrhagic fever with renal syndrome in khabarovsk territory]. 19901973013
[cases of hemorrhagic fever with renal syndrome in amur province associated with the rat serotype of the virus].to carry out serodiagnosis and to determine the serotype of the virus causing hemorrhagic fever with the renal syndrome (hfrs), paired sera obtained from 28 hfrs patients, 42 persons with a history of hfrs (1-17 years after convalescence), and 268 serum samples from healthy persons residing at the areas with the natural foci of this infection have been studied by indirect immunofluorescence techniques at the territory of the amur province. this study has demonstrated for the first time that, alo ...19901973327
outbreak of hemorrhagic fever with renal syndrome among u.s. marines in korea.fourteen of 3,754 u.s. marines who participated in a joint united states-republic of korea training exercise during the autumn of 1986 developed hemorrhagic fever with renal syndrome (hfrs). clinical and laboratory findings among cases included fever, headache, fatigue, gastrointestinal dysfunction, thrombocytopenia, and proteinuria. ten individuals were hospitalized; 2 died. no subclinical infections were identified through a post-deployment screen of sera obtained from 2,053 exercise participa ...19901973603
hemorrhagic fever with renal syndrome imported to hawaii from west germany. 19901974384
the protean manifestations of hemorrhagic fever with renal syndrome. a retrospective review of 26 cases from korea.twenty-six cases of hemorrhagic fever with renal syndrome from 1981 to 1986 were retrospectively reviewed to determine the scope of clinical presentation and the unique complications of the illness. the diagnosis was confirmed by detection of hantaan virus antibody in 25 cases and by characteristic autopsy findings in 1 case. the illness could be classified into three distinct clinical subgroups. fever was universally present. two patients presented with intractable shock and diffuse hemorrhage ...19901974404
hantavirus disease. 19901974945
[preliminary establishment of hybridoma secreting monoclonal anti-idiotypic antibodies to hemorrhagic fever with renal syndrome virus]. 19901975220
isolation of a strain of hantaan virus from peritoneal exudate cells of a patient with hemorrhagic fever with renal syndrome.a new strain of hantaan virus (htnv), gh716, was isolated from the peritoneal exudate cells (pec) of a patient with severe hemorrhagic fever with renal syndrome (hfrs). the isolate was propagated in vero e6 cells. at each passage the virus-infected cells were examined for htnv with immunofluorescence technique using monoclonal antibodies (mcab) 25-1 and 84-1 against htnv. from passage 5 on, fluorescent intensity tended to stabilize at (+++) and infectivity titer reached 10(5) tcid50/ml. using 14 ...19901976488
[the role of biologically active substances in permeability changes in the blood vessels of patients with hemorrhagic fever with renal syndrome].to judge the possible causes of the impairment of blood vascular permeability in hemorrhagic fever associated with the nephrotic syndrome (hfns), 86 patients were examined over time for the blood content of histamine, serotonin and kallikrein. the patients were found to have hyperhistaminemia, hyperserotoninemia and activation of blood serum kallikrein, which attests to undoubted participation of these biologically active substances in the pathogenesis of hfns.19901977202
[the thrombocyte link in the hemostasis of patients with hemorrhagic fever with renal syndrome].altogether 38 patients (33 men and 5 women) suffering from grave and moderate hemorrhagic fever with nephrotic syndrome (hfns) were examined. the hemolysate aggregation test and adp were employed to explore the aggregation ability of platelets in those patients. besides, measurements were made of the activity of the 3rd platelet factor in the plasma and serotonin content in each platelet. a considerable rise of the aggregation activity of platelets was established to take place in the oligoanuri ...19901977203
[vascular permeability, microcirculation and biologically active substances in patients with hemorrhagic fever with renal syndrome].as many as 56 patients with associated hemorrhagic fever and the renal syndrome were examined for vascular permeability, microcirculation and blood plasma histamine, serotonin, kallikrein and prekallikrein. in all the disease periods, the majority of the patients (n-52) demonstrated a rise of vascular permeability for protein and liquid part of the plasma, with this being marked in particular in the oligoanuric period. besides, significant perivascular and intravascular changes in microcirculati ...19901977204
[the cohort study on hfrsv antibody variation in normal population of an epidemic area].we randomly selected 2,796 persons in an epidemic area of hfrs in anhui province for serological study from april to december in 1988. the results showed that the antibody prevalence rates were 7.3%, 10% and 14% respectively in april, september and december. their gmt were 1.30, 1.42 and 1.73 respectively. indoor rodent density and hfrsv carrying rate were respectively 7.8%, 6.4% in april, 9.4%, 8.8% in september, and 7.5%, 8.2% in december. outdoor rodent density and hfrsv carrying rate were re ...19901977521
[the serological diagnosis of hantaan virus infections]. 19901977569
[the clinical picture and therapy of hantaan virus infection]. 19901977570
a retrospective analysis of sera collected by the hemorrhagic fever commission during the korean conflict.more than 600 sera from 245 patients with a clinical diagnosis of hemorrhagic fever were preserved by the hemorrhagic fever commission during the korean conflict, 1951-1954. these sera were tested for igm- and igg-specific antibodies to hantaan virus by enzyme immunoassay and for hantaviral antigen by immunoassay; one serum from each patient was tested by plaque reduction neutralization using both hantaan and seoul viruses. only 15 patients failed to develop antihantaviral antibodies; most sera ...19901977805
[epidemiology of hemorrhagic fever with renal syndrome in the ardennes region]. 19901978307
[hemorrhagic fever with renal syndrome in yugoslavia 1951-1988].data on hemorrhagic fever with renal syndrome (hfrs) incidence in sfry 1950-1988 were analysed. information sources were published papers and official reports of federal and republic institutions for public health. indirect immunofluorescence technique was used in testing 1.842 organs of small wild mammals to the presence of hfrs viral antigen. this antigen was found in the lungs of 11 species. average incidence of the carrier state was 10.4% registered were 613 cases within the period 1951-1988 ...19901978438
hantavirus infection presenting as acute renal failure. 19901978888
[hantaan virus serotypes circulating in foci of the far eastern region of the ussr].studies on serodifferentiation by indirect immunofluorescence antibody method and neutralization test of hantaan virus strains isolated from human patients and rodents revealed circulation on the maritime territory of 5 virus serotypes and demonstrated that rodents of one species could be virus carriers of different virus serotypes. on the basis of the antigenic similarity of strains isolated from human patients, field mice, and clethrionomys rufocanus it is concluded that the virus serotypes ci ...19901980045
different pathohistological presentations of acute renal involvement in hantaan virus infection: report of two cases.we present two patients with hantaan virus infection, admitted to the department of nephrology, skopje, at the same time, with the same clinical presentation (chills, fever, abdominal pain, hemorrhages, nausea, headache, proteinuria, hematuria, oliguria, acute renal failure) but with different pathohistological findings and different disease courses. in the first case diffuse proliferative glomerulonephritis was found, with a complete recovery of renal function after a month, with a mild protein ...19901980098
[the participation of histamine and serotonin in the genesis of acute kidney failure in patients with hemorrhagic fever with renal syndrome].in order to explore possible involvement of histamine and serotonin in acute renal failure related to hemorrhagic fever with renal syndrome (hfrs), selected markers or renal lesions have been compared with biochemical findings in highly severe cases. literature reports and the study results lead the authors to conclude that histamine and serotonin are obviously pathogenetic determinants of hfrs since their concentrations in plasma of these patients are increased 3-fold. it is suggested that the ...19901980387
[experiment research on moxibustion treating infected ehfv rat].in this experiment, we established a rat model of ehfv infected by abdominal cavity, and observed the effect of moxibustion on the changes of antigens, antibodies, neural transmitters in blood and tissue on 60 weanling wistar rats with ehf. the rats were divided into three groups: normal control group (n. group), ehf control group (c. group) and ehf with moxibustion group (m. group) at random. moxibustion was given at "shenshu" for 30 minutes daily, 7 days. the 14th day after infection of ehfv, ...19901980432
[epidemiologic characteristics of hemorrhagic fever with renal syndrome in a disease reservoir near foca]. 19901981115
[diagnosis of hemorrhagic fever with renal syndrome].the authors observed 22 patients with hemorrhagic fever with the renal syndrome (hfrs). the diagnosis turned out to be difficult in outpatient clinics as all the patients were referred to hospital with erroneous diagnoses. epidemiological causes of infection of hfrs were indicated. the authors singled out 3 periods in a course of this disease with characteristic symptoms. the diagnosis was confirmed serologically in the reaction of indirect immunofluorescence, a 4-fold increase and over in antib ...19901981246
hantavirus infection in laboratory and wild rodents in argentina.serum samples from urban and laboratory rats, laboratory mice and wild and laboratory cricetids in argentina were tested by immunofluorescence and plaque reduction neutralization tests to investigate prevalence of anti-hantavirus antibodies. a total of 102 sera were obtained from laboratory rodents in 4 different animal-rooms, 31 from harbor rats and 30 from wild cricetids in 1985-1987. anti-hantavirus antibodies were detected in 22.5% of rattus norvegicus in 3 of the animal-rooms but harbor rat ...19901981381
detection of antibodies to the western and the eastern type of haemorrhagic fever with renal syndrome in patient's sera from slovakia.we present serological evidence of infection with western and eastern type of haemorrhagic fever with renal syndrome (hfrs) virus in patient's sera from slovakia by indirect immunofluorescence. treatment of sera with 2-mercaptoethanol decreased levels of haemagglutination-inhibition (hi) antibodies to the eastern type of hfrs suggesting primary infection of the patients by this virus.19901981450
[the role of hantaan virus serotypes in the etiology of hemorrhagic fever with renal syndrome in the far east of the ussr].the employment of two serological tests (indirect fluorescence antibody technique and neutralization test) demonstrated the leading role of hantaan virus serotype 1 strains isolated from field mice in the pattern of the incidence of hemorrhagic fever with renal syndrome (hfrs). proofs of the etiological importance of strains of serotypes 3 and 5 occurring in brown rats and cl. rufocanus were obtained. no data on any association of human hfrs cases with strains of serotype 4 isolated from reed vo ...19901982032
haemorrhagic fever with renal syndrome: evaluation of elisa for detection of puumala-virus-specific igg and igm.igm and igg elisa to puumala virus were evaluated using sera from patients with haemorrhagic fever with renal syndrome (hfrs) from different geographical regions: sweden, denmark, norway, belgium and the european ussr. igm elisa proved useful in the diagnosis of hfrs in patients from all the regions mentioned above. specific igm could be detected as early as day 1 post onset of disease, and patients remained igm-positive for several months. specific igg elisa antibodies were also frequently dete ...19901982371
[spontaneous rupture of the kidneys in hemorrhagic fever with renal syndrome].during the recent 5 years we had 29 patients with hemorrhagic fever and renal syndrome complicated by a spontaneous kidney rupture. this complication occurred during an anuric period in 18 patients and within the first 24 hours of restored diuresis in 11 patients. major clinical presentations of the kidney rupture were deterioration of lumbar and abdominal pains, meteorism and symptoms of an internal bleeding. palpation revealed hematoma at the site of the ruptured kidney, tension of the anterio ...19901982474
[epidemiological survey of epidemic haemorrhagic fever in xianning county].the incidence, distribution pattern and trend of disease spreading of ehf in xianning country, hubei province during 1971-1985 are described. serologic study showed that the serum antigen positivity rate was 12.90% for apodemus agragrius and 6.67% for rattus norvegicus and that the serum antibody positivity rate was 61.54% in fhf patients and 30.85% in the exposed contacts of the patients' families in the epidemic area. in 1985, the hfrs virus a24 was isolated from apodemus agragrius and identif ...19901983075
[a ten-year retrospective investigation of serum against ehf antibodies in nanchong district, sichuan].this paper reports a retrospective inquiry of blood serum against epidemic hemorrhagic fever (ehf) antibodies in some of our hospital treated ehf patients during 1974-1984. it was found that the total positive rate of sera against ehf antibodies obtained from different periods of ehf patients was 86.8% (170/196). the titers were highest in patients who had been attacked within one year and the titers markedly decreased from the second year onwards. however certain level of serum titre could be d ...19901983076
primary structure of the large (l) rna segment of nephropathia epidemica virus strain hällnäs b1 coding for the viral rna polymerase.the l rna segment of the nephropathia epidemica virus (nev) strain hällnäs b1 was characterized by molecular cloning of the corresponding cdna and subsequent determination of the dna nucleotide sequence. the l rna segment is 6550 nucleotides long with complementarity of 20 bases at the 3' and 5' termini. the viral messenger sense rna contains one major open reading frame (orf) with a coding capacity of 2156 amino acid residues encoding a protein with a calculated molecular weight of 246 kda and ...19912053288
molecular characterization of the rna s segment of nephropathia epidemica virus strain hällnäs b1.the s segment rna of nephropathia epidemica virus (nev) strain hällnäs b1 was isolated by molecular cloning of the corresponding cdna. the rna is 1785 nucleotides long with the 3' and 5' termini being complementary for 23 bases. the viral messenger-sense rna contains one major open reading frame (orf) with a coding capacity of 433 amino acids encoding a 49-kda polypeptide. compared to the hantaan s segment cdna sequence there is a nucleotide homology of 60 and 61% at the amino acid level. many o ...19902104684
experimental infection of human vascular endothelial cells by pathogenic and nonpathogenic investigate whether the hantaviruses replicate in endothelial cells, we inoculated human umbilical vein endothelial cells with several pathogenic and nonpathogenic strains of hantavirus. intracytoplasmic, virus-specific granular fluorescence was detected initially at three days postinoculation and nearly 100% of cells contained viral antigen at 10 days. cytopathic effect or inclusion bodies were not observed. the in vitro demonstration of the susceptibility of endothelial cells to hantaviruse ...19902112908
[virus-specific proteins and rna of the virus of hemorrhagic fever with renal syndrome].virus-specific proteins g1, g2, and n with molecular weights of 70, 55-57, and 50 kilodaltons, respectively, were detected by radioimmunodiffusion tests in vero e-6 cells infected with strains of virus of hemorrhagic fever with renal syndrome (hfrs) isolated in the european ussr from a patient with hfrs, a fatal human case of hfrs (the strains k-27 and p-360) and from a bank vole (strain cg-1820). the sera from human convalescents after hfrs in the european ussr and rat sera prepared with the cg ...19902114054
nucleotide sequence of the m genome segment of hemorrhagic fever with renal syndrome virus strain b-1. 19902118626
hantavirus strains isolated from rodentia and insectivora in rural china differentiated by polymerase chain reaction assay.polymerase chain reaction (pcr) assay and other techniques were applied to differentiate hantavirus strains isolated from different animal hosts and geographic regions in china. two groups of related strains, hantaan and seoul, have been classified by cross-neutralization, radioimmunoprecipitation (rip), and sodium dodecyl sulfate polyacrylamide gel electrophoresis (sds-page) assays. the molecular weights of glycoprotein 1 (g1) of hantaan and seoul viruses were 72k and 80k, whereas those of the ...19902123383
zoonotic infections in northern ireland farmers.evidence of past zoonotic infection was investigated serologically in randomly selected northern ireland farmers. the percentage of farmers with antibody was: brucella abortus (0.7), leptospira interrogans serovars (8.1), borrelia burgdorferi (14.3), toxoplasma gondii (73.5), coxiella burnetii (28.0), chlamydia psittaci (11.1) and hantavirus (1.2). the results show that northern ireland farmers have been exposed in the past to zoonotic infections. it is not known if these infections contributed ...19902123457
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