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detection of the antigen and antibodies to the eastern subtype of haemorrhagic fever with renal syndrome virus in small rodents in slovakia.direct enzyme-linked immunosorbent assay (elisa) was used for the demonstration of haemorrhagic fever with renal syndrome (hfrs) virus antigen in lung tissue of small rodents trapped in eastern and western slovakia. the eastern subtype of hfrs virus antigen was demonstrated in the lungs of apodemus agrarius and of the western subtype in the lungs of microtus arvalis. antibodies to hfrs virus antigen have been detected in apodemus species (a. agrarius and a. flavicollis) in higher titres to the e ...19882899965
antigenic relationship between hantaviruses analysed by immunoprecipitation.antigenic relationships between seven hantaviruses isolated in sweden, belgium, korea, european u.s.s.r. and asian u.s.s.r. were studied by radioimmunoprecipitation assays (ripa) and indirect immunofluorescence tests (ift). animal immune sera and haemorrhagic fever with renal syndrome (hfrs) patient sera from the above countries were used. the strains fell into three antigenic groups by both ripa and ift: nephropathia epidemica (ne)-type, korean haemorrhagic fever (khf)-type and urban rat-type. ...19882902191
detection of nephropathia epidemica (puumala virus)-specific immunoglobulin m by enzyme-linked immunosorbent assay.nephropathia epidemica (ne), a less severe form of hemorrhagic fever with renal syndrome, is caused by puumala virus (puu). this communication reports the development of a mu-capture enzyme-linked immunosorbent assay (elisa) for detection of specific immunoglobulin m (igm) antibodies to puu virus in human sera. acute- and early-convalescent-phase sera (collected 1 to 41 days after disease onset) from 29 swedish patients with clinical ne were tested for puu virus-specific igg and igm antibodies b ...19882902106
seroepidemiology of haemorrhagic fever with renal syndrome in bulgaria.during the period of 1954-1986, 399 cases of haemorrhagic fever with renal syndrome (hfrs) were registered in bulgaria with 63 (15.7%) deaths. three hundred serum samples from 214 patients who had contracted the disease from 1957 to 1986 were investigated by indirect fluorescent antibody test (if-at). as antigen vero-e6 cells infected with the asian hantaan virus were used, as well as lung sections from bank voles (clethrionomys glareolus) infected with strains udmurt and kazan 6-cg from the eur ...19882902774
a simple method of preparing a complement-fixing antigen from the virus of haemorrhagic fever with renal syndrome (western type).different methods of the preparation of haemorrhagic fever with renal syndrome (hfrs) antigen for complement-fixation (cf) test are described. the antigens were prepared from the organs of suckling white rats inoculated with the western type of hrfs by precipitation with polyethylene glycol, by fluorocarbon treatment and or by sucrose-acetone extraction. the highest cf titre was obtained by acetone precipitation of 20% brain suspension in isotonic sucrose.19882902776
hantavirus disease in sri lanka. 19882904003
virus-specific proteins and rnas from cells infected with hemorrhagic fever with renal syndrome (hfrs) viruses derived from endemic areas of the u.s.s.r.cell-associated proteins of rodent (cg-1820 strain) and human (k-27 and 360 strains) hantaviruses isolated in the european endemic areas of the u.s.s.r. are antigenically similar as revealed by immunoprecipitation and immunoblotting assays. nucleocapsid-associated rnas of representative hantaviruses of four antigenic groups [sugiyama et al. (1987) j gen virol 68: 979-987] have unique page patterns. "electropherotyping" of the rnas isolated from infected cells might be used for identifying and di ...19882904252
hantaviruses are here in scotland. 19882904932
status of hantavirus in the central african republic. 19882905142
[beta2-microglobulin in the blood serum in hemorrhagic fever with the renal syndrome]. 19882905527
a negative human serosurvey of haemorrhagic fever viruses in djibouti. 19882905609
nephropathia epidemica encephalitis.a case of serologically proven nephropathia epidemica (ne) with encephalitis is described. follow up of serum and csf antibody titres demonstrated ne virus antibody production in the cns concomitantly with clinical encephalitis. decrease of initially high csf antibody titres was followed by a simultaneous increase of serum antibody titres corresponding to the clinical picture shifting from encephalitis to nephritis. this suggests that the first replication of ne virus may undertake in the cns.19882906497
serological evidence of hantavirus infection in laboratory rats and personnel.laboratory-acquired haemorrhagic fever with renal syndrome (hfrs) has been reported in many countries. a serological survey of laboratory white rats and of laboratory personnel for antibodies to hantaviruses was conducted in singapore. forty-four per cent (143/329) of rats were seropositive by the indirect immunofluorescent antibody test but none had hantaviral antigens in lung tissues. two of 74 laboratory personnel were seropositive but neither had a history of clinical illness. the high serop ...19882906333
[study on the surveillance of house-rat type epidemic haemorrhagic fever]. 19882905938
study of hamsters infected with epidemic hemorrhagic fever virus. 19882906853
[detection of antigens in blood smears of patients with epidemic hemorrhagic fever using the rosette cell formation spa method]. 19882907414
[first report of an endemic area of epidemic hemorrhagic fever in boarding prefecture]. 19882905939
[detection of igg antibody in serum of hemorrhage fever with renal syndrome (hfrs) by solid-phase chemiluminescence immunoassay]. 19882906571
aerosol transmission of hantaan and related viruses to laboratory rats.hantaan, seoul, and puumala viruses were transmitted to 12-16-week-old female wistar rattus norvegicus by inhalation. the rodent infectious dose for each virus by intramuscular inoculation and by inhalation was determined, as was the infectious dose for vero e-6 cells by direct plaque assay.19882908582
[study of dog as the animal reservoir of hemorrhagic fever with renal syndrome]. 19882906570
[serological demonstration of the presence of hantaan virus in rattus norvegicus rats in a rural environment in france]. 19872951694
[persistent infection caused by hemorrhagic fever with renal syndrome virus in bank voles (clethrionomys glareolus)--the natural host of the virus]. 19872907924
[serological demonstration of the presence of hantaan virus in rattus norvegicus rats in an urban environment in france]. 19872951696
[determination of specific immune complexes and dynamics of their circulation in patients with hemorrhagic fever with renal syndrome]. 19872907925
[detection of antigens and analysis of antigenicity of hemorrhagic fever with renal syndrome viruses by immune adherence hemagglutination assay (iaha)]. 19862954670
parasites, diseases and health status of sympatric populations of sambar deer and white-tailed deer in florida.from december 1983 to december 1984 a study on parasites, diseases and health status was conducted on sympatric populations of sambar deer (cervus unicolor) and white-tailed deer (odocoileus virginianus) from st. vincent island, franklin county, florida. ten sambar and six white-tailed deer were examined. white-tailed deer had antibodies to epizootic hemorrhagic disease virus and bluetongue virus. serologic tests for antibodies to the etiologic agents of bovine virus diarrhea, infectious bovine ...19873035242
[immunocolloidal gold labelling electron microscopy and its application in studying the morphology of viruses]. 19873036385
[isolation of strains of the hemorrhagic fever with renal syndrome virus in a cell culture]. 19872977813
hantaan virus replication: effects of monensin, tunicamycin and endoglycosidases on the structural glycoproteins.the monovalent ionophore monensin, which interferes with cellular transport pathways, and the antibiotic tunicamycin, which prevents glycosylation of newly synthesized proteins, were used to examine hantaan virus particle formation and polypeptide synthesis. viral replication in the presence of either drug resulted in reduced antigen production as well as reduced yields of both intracellular and extracellular infectious virus. analysis of viral polypeptides synthesized in the presence of the dru ...19863083039
hemorrhagic fever with renal syndrome in czechoslovakia: detection of antigen in small terrestrial mammals and specific serum antibodies in man.a direct elisa technique was used for demonstration of hfrs virus antigen in suspensions of the lungs obtained from 774 wild small mammals belonging to 13 zoological species trapped in 21 localities in czechoslovakia between 1983 and 1984. western serotype of hfrs virus was demonstrated in the lungs of 8 microtus arvalis voles caught in the areas of central bohemia, southern moravia and western slovakia, and in one clethrionomys glareolus vole from the area of eastern slovakia. the eastern serot ...19863084630
[hemorrhagic fever viruses. principal epidemiologic aspects]. 19873038354
[detection of antibodies to viruses causing hemorrhagic fever associated with a renal syndrome]. 19863089543
a preliminary report of a comparison of epizootic haemorrhagic disease viruses from australia with others from north america, japan and nigeria. 19863022701
[evaluation of the results of a direct solid-phase immunoenzyme method for determining antigens].aspects of an objective analysis of the results of solid-phase enzyme-immunoassay (speia) for identification of antigens (a direct version) were considered on the model of the virus of hemorrhagic fever with renal syndrome. using a statistical method, certain parameters of 11 types of polystyrene and polyvinylchloride microplates (solid carrier) of different manufacturers were studied from the point of view of their suitability for obtaining reproducible speia results. statistical methods for th ...19863099475
propagation of epidemic hemorrhagic fever virus in meriones unguiculatus. 19863100239
[strains of epidemic hemorrhagic fever virus isolated from the lungs of c. russula and a. squamipes]. 19863100244
hantaan virus m rna: coding strategy, nucleotide sequence, and gene order.the m genome segment of hantaan virus was molecularly cloned and the nucleotide sequence of cdna was determined. the virion rna is 3616 bases long with 3'- and 5'-terminal nucleotide sequences complementary for 18 bases. a single long open reading frame in the viral complementary-sense rna had the potential to encode 1135 amino acids or a polypeptide of 126,000 da. amino-terminal sequences of isolated g1 and g2 envelope glycoproteins were determined, revealing a gene order with respect to messag ...19873103329
four serotypes of haemorrhagic fever with renal syndrome viruses identified by polyclonal and monoclonal antibodies.antigenic relationships among 20 strains of haemorrhagic fever with renal syndrome (hfrs) viruses isolated in korea, china, u.s.s.r., finland, japan and u.s.a. were examined with rat immune sera, patient sera, eight monoclonal antibodies against the sr-11 strain and 10 monoclonal antibodies against the 76-118 strain. antigen analyses by indirect immunofluorescent antibody and immune adherence haemagglutination tests using polyclonal and monoclonal antibodies demonstrated that hrfs viruses may be ...19873106565
epizootiology of hantavirus infections in baltimore: isolation of a virus from norway rats, and characteristics of infected rat populations.rats trapped from 14 locations in baltimore, maryland, were shown to have antibody to hantavirus. antibody prevalence rates were higher in residential locations than in parks. infected rats were obtained continuously over a six-year period from 1980-1985 at five locations, indicating the enzootic nature of this infection in urban rats in the united states. prevalence of antibody and geometric mean titers increased with rat mass and sexual maturity, suggesting that infections in rats are acquired ...19873109236
antibody to japanese strain of haemorrhagic fever with renal syndrome (hfrs) virus in nigerian sera. 19863111024
nucleotide sequence of the m segment of the genomic rna of hantaan virus 76-118. 19873114716
coding strategy of the s genome segment of hantaan virus.hantaan virus is the type species of the recently recognized hantavirus genus of bunyaviridae. the small (s) rna segment of the negative-sense, tripartite genome was molecularly cloned and the nucleotide sequence was determined. the rna sequence derived from the cdna copy was found to contain 1696 nucleotides. a single open reading frame of sufficient size to encode the virus nucleocapsid protein was detected in the cdna corresponding to viral complementary-sense rna. rna transcripts of the cdna ...19863024404
serological evidence of hantaan-related viruses in hong kong.sera from rats (rattus norvegicus), domestic animals and man in hong kong were studied by immunofluorescent antibody assay and plaque reduction neutralization tests for evidence of infection by hantaan-related viruses. antibody probably arising from infection by seoul or a seoul-like virus was detected in rat and human sera (and one pig serum), highlighting the potential public health importance of this group of viruses in hong kong, and in the region.19873120366
seroepidemiology of hantaan-related virus in gabon. 19873120374
expression of the hantaan virus m genome segment by using a vaccinia virus recombinant.a cdna containing the complete open reading frame of the hantaan virus (htn) m genome segment has been cloned into vaccinia virus. this recombinant virus expresses two glycoproteins which are similar to the htn structural glycoproteins, g1 and g2, in molecular weight, cleavage pattern, and cellular distribution. both htn and recombinant vaccinia virus glycoproteins are exclusively associated with the golgi apparatus of the cell. despite this intracellular restriction, mice inoculated with the re ...19883123711
[detection of antigens and antibodies of epidemic hemorrhagic fever virus in experimental albino rats in guangzhou city]. 19863091256
[carriers of epidemic hemorrhagic fever virus in rodents and mites in ehf endemic area]. 19863087624
evidence of human infection with a rat-associated hantavirus in baltimore, maryland.human sera obtained from two sources in baltimore were tested for antibodies against a hantavirus by a variety of techniques. four persons out of 1,788 had hantaviral antibody as demonstrated by immunofluorescent assay, enzyme-linked immunosorbent assay, and neutralizing assay. neutralizing antibody titrations against three test viruses indicated that infections were caused by a rat-associated virus. medical histories of two subjects indicated life-long residence in baltimore and an absence of f ...19883128102
viruses of hemorrhagic fever with renal syndrome (hfrs) grouped by immunoprecipitation and hemagglutination inhibition.nine viruses antigenically related to hantaan virus that were isolated from various rodents in several countries and from a patient were examined by immunoprecipitation and by hemagglutination inhibition (hi). the immunoprecipitation tests were done with a monoclonal antibody that reacted with glycoprotein g2 and an antibody against a nucleocapsid protein. the viruses were classified into four subtypes on the basis of the molecular weights of their precipitated polypeptides and the pattern of re ...19873117723
cell-mediated immunity to virus causing haemorrhagic fever with renal syndrome: generation of cytotoxic t lymphocytes.cytotoxic t lymphocytes (ctls) were generated when spleen cells from mice infected with viruses causing haemorrhagic fever with renal syndrome were stimulated in vitro with syngeneic cells infected with viruses. these cytotoxic effector cells, with lyt2+ l3t4- markers on their surface, demonstrated h-2 restriction. ctls induced by hantaan virus (76-118 strain) or seoul virus (b-1 strain) showed cross-reactivity with infected target cells. hantaan virus infection induced a higher ctls response th ...19883137309
[identification of epidemic hemorrhagic fever virus in human leukocytes]. 19873127020
protein coding assignment for the genome of epizootic haemorrhagic disease virus.viral genomic rna was purified from bhk-21 cells infected with epizootic haemorrhagic disease virus and the 10 dsrna genome segments were isolated by polyacrylamide gel electrophoresis. these genome segments were translated in vitro using the rabbit reticulocyte lysate system and the synthesized proteins were detected by immune precipitation and gel electrophoresis. this allowed the assignment of protein coding to the genome segments and the identification of two additional virus-specified prote ...19883133450
association of intraspecific wounding with hantaviral infection in wild rats (rattus norvegicus).the potential for hantaviral transmission among wild norway rats by wounding associated with aggressive interactions was evaluated using a prospective sero-epidemiological study coupled with a mark-release-recapture survey. there was a significant association between an animal's serological status and the presence of wounds. longitudinal studies of marked and released animals showed seroconversion between captures was associated with wounding between captures more often (33%) than expected by ch ...19883141203
leakey virus: a new hantavirus isolated from mus musculus in the united states.a hantavirus, designated leakey virus, was isolated from a mus musculus captured in real county, texas, u.s.a. in august 1986. virus-specific fluorescence was first detected 13 days after inoculation of vero-e6 cells with spleen tissue from the seropositive m. musculus. ultrastructurally, the new isolate resembled other hantaviruses. leakey virus induced a fatal meningoencephalitis in infant fischer rats, with viral antigen detectable in brain, lung, liver, kidney and spleen. serum dilution, pla ...19883143810
use of heat inactivated viral haemorrhagic fever antigens in serological assays.heating for 1 h at 60 degrees c completely destroyed the infectivity of sucrose-acetone-extracted antigen of rift valley (rvf) and congo crimean haemorrhagic fever (cchf), as well as of rvf- and cchf-infected mouse brain. these antigens could be successfully used, however, for complement fixation and igm-capturing enzyme immunoassay. vero e6 cell suspensions infected with hantaviruses such as hantaan 76-118, tchoupitoulas, sr 11, gb-b, cg 18-20, hällnäs, cg 13891, seoul and prospect hill, as wel ...19883146583
[propagation and replication of hantaan virus in human endothelial cells in vitro]. 19883147808
coagulopathy in patients with hemorrhagic fever with renal syndrome.hemorrhagic fever with renal syndrome in korea (korean hemorrhagic fever) is an acute viral disease characterized by fever, hemorrhage and renal failure. in korean patients, the disease manifests more distinctive bleeding tendencies than those of hemorrhagic fever with renal syndrome found in western countries. to investigate the nature and role of the coagulation, fibrinolysis, kinin and immune system in the pathogenesis of such a hemorrhagic manifestation, alterations of these systems were ass ...19873151765
[use of monoclonal antibodies for differentiating strains of the virus causing the hemorrhagic fever with renal syndrome].using monoclonal antibody and animal immune sera the experiment confirmed the existence of antigenic relationships between the strains of virus of hemorrhagic fever with renal syndrome (hfrs) isolated in the ussr from clethrionomys glareolus and members of all known serotypes of hfrs virus. clear-cut differentiation was made from serotypes apodemus and rattus, and cross-relationships were shown between the strains isolated in the ussr and prospect hill virus (phv), a member of the serotype micro ...19873124351
reservoir animals for nephropathia epidemica in norway: indications of a major role for the bank vole (c. glareolus) in comparison with the woodmouse (a. sylvaticus).small rodents were collected live in two different locations within a nephropathia epidemica (ne) endemic area, and tested for both antiviral serum antibodies and viral antigens in lung sections. in one location, only apodemus sylvaticus (woodmice) were found in the traps, in the other, both a. sylvaticus and clethrionomys glareolus (bank voles) were collected. among the woodmice from the former location the prevalence of ne virus markers was significantly lower than for either woodmice or bank ...19853919085
isolation of haemorrhagic fever with renal syndrome virus from leukocytes of rats and virus replication in cultures of rat and human macrophages.newborn rats were inoculated intraperitoneally with haemorrhagic fever with renal syndrome (hfrs)-related virus (b-1 strain), and virus isolation from their various organs was attempted between 1 and 25 weeks after inoculation. virus could be isolated repeatedly from lung, brain, spleen and kidney and also from peripheral blood. when virus isolation was carried out on fractionated peripheral blood cells, virus was associated mainly with the macrophage fraction and to a lesser extent with granulo ...19853925075
development of inactivated vaccine against virus causing haemorrhagic fever with renal syndrome.b-1 virus belonging to the hantavirus group was serially passaged in the brains of newborn mice. inactivated vaccine was prepared from the brains after inactivation with formalin and then purification by ultracentrifugation. the antigenic potency of this vaccine in vitro was determined by antibody-bound enzyme-linked immunosorbent assay (elisa) and serial diluted vaccine bound to an aluminium hydroxide gel was inoculated into balb/c mice to test immunogenicity. after two injections of this vacci ...19883138829
partial characterization of prospect hill virus isolated from meadow voles in the united states. 19853930622
certified cell lines of rat origin appear free of infection with hantavirus. 19853930626
serologic evidence of hantaan-like virus in rodents and man in thailand.a serologic survey was conducted to determine the presence of antibody to hantavirus in rodents in thailand. sera from over 300 rodents were tested by an immunofluorescent antibody method. bandicota indica, a field rodent, was found to have a high incidence of infection (20-24%) in 2 locations. a hantavirus was isolated from lung samples of b. indica. when sera were tested from humans living in kanchanaburi and several locations in bangkok, those people living in close proximity to the infected ...19853938073
experimental hantavirus infection in nonhuman primates.mild, transient proteinuria and azotemia were produced in three cynomolgus monkeys (macaca fascicularis) and a chimpanzee (pan troglodytes) following intravenous inoculation with prospect hill virus, a hantavirus isolated from meadow voles in the united states. this is the first demonstration of an acute nephropathy in nonhuman primates with the viruses causing hemorrhagic fever with renal syndrome.19883137914
[indirect hemagglutination reaction with chf-congo group viruses]. 19744217050
definition of three serotypes of hantaviruses by a double sandwich elisa with biotin-avidin amplification system.a total of seven different isolates of hantaviruses from five mammalian species and six geographical areas were examined by a double sandwich elisa with biotin-avidin amplification system. three serotypes were recognized by comparing antigen titres to homologous and heterologous antibody and blocking antibody titres to homologous and heterologous antigens. serotype 1 included four strains: hantaan virus isolated from apodemus agrarius in south korea, hupei-i isolated from acute-phase serum from ...19853926952
immunosuppression by junin virus. ultrastructural study. 19744213176
aetiological relation between korean haemorrhagic fever with renal syndrome in people's republic of china. 19806102694
identification of epidemic haemorrhagic fever with renal syndrome in china with korean haemorrhagic fever. 19806103345
scandinavian epidemic nephropathy and korean haemorrhagic fever. 19806105239
antigenic differentiation of the viruses causing korean haemorrhagic fever and epidemic (endemic) nephropathy of scandinavia. 19806105459
propagation of korean hemorrhagic fever virus in laboratory rats.korean hemorrhagic fever virus (khfv) has been adapted to the wistar and fisher strains of rats. infection was detected by the appearance of specific antigen in the lung tissue of the infected rats at 14 to 64 days after inoculation and by the appearance of circulating antibodies in ther serum which reacted specifically with khfv antigen in the lungs of infected apodemus agrarius subsp. coreae 3 weeks after inoculation. distribution of antigen in rat tissues as determined by immunofluorescent st ...19816111538
serological evidence for korean haemorrhagic fever in greece. 19816111686
korean hemorrhagic fever: propagation of the etiologic agent in a cell line of human origin.the etiologic agent of korean hemorrhagic fever has been propagated in a human cultured cell line derived from a carcinoma of the lung. the cells, described as type ii, alveolar epithelial, support replication of the agent and successive passages. antigen of the korean hemorrhagic fever agent is readily detected in infected cells by means of direct or indirect fluorescent antibody techniques. previous attempts to propagate this agent in vitro had been unsuccessful.19816110243
are there two serotypes of the agent of haemorrhagic fever with renal syndrome? 19806106800
electron microscope appearance of hantaan virus, the causative agent of korean haemorrhagic fever.the morphology and morphogenesis of three strains of hantaan virus, which causes korean haemorrhagic fever (khf), were examined by thin-section and negative-contrast electron microscopy of infected a549 cell-culture specimens. in thin sections, virus was detected within cytoplasmic granular matrices (viroplasms) of the infected cells. virus particles were spherical (diameter 73 +/- 5 nm), and had an extremely electron-dense core (diameter 47 +/- 6.5 nm). replication and maturation of the virus s ...19816112446
detection by enzyme-linked immunosorbent assay of haemorrhagic fever with renal syndrome virus in lung tissue of rodents from european u.s.s.r. 19816112426
[detection of the etiologic agent of hemorrhagic fever with a renal syndrome in the lungs of wild rodents].the results of the studies on wild rodents trapped in the areas of the leningrad region visits to which were associated with human cases of hemorrhagic fever with renal syndrome (hfrs) are presented. in one of these areas 30% of bank voles were found to be infected. specific fluorescence of high intensity was found in ctyostat sections of lungs from bank voles treated by the indirect fluorescent antibody procedure. fluorescent granules, their clumps and large conglomerates were localized in the ...19816115516
morphology and morphogenesis of viruses of hemorrhagic fever with renal syndrome. ii. inclusion bodies--ultrastructural markers of hantavirus-infected cells.three distinctive types of viral inclusion bodies were identified in hantavirus-infected cells by thin-section electron microscopy. the inclusion bodies, designated granular, granulofilamentous, and filamentous, were intracytoplasmic and closely associated with the rough endoplasmic reticulum and golgi cisternae. virus specificity of the inclusions was verified by immune colloidal gold and immunoperoxidase labeling. the inclusion bodies were a common morphological marker for 13 strains of hantav ...19873610571
intraspecific transmission of hantaan virus, etiologic agent of korean hemorrhagic fever, in the rodent apodemus agrarius.experimental parameters of infection and intraspecific transmission of hantaan virus, the etiologic agent of korean hemorrhagic fever, in apodemus agrarius rodents were determined. mice inoculated by the intramuscular route experienced viremia for about 1 week beginning on day 7. after 3 weeks, immunofluorescent and neutralizing antibodies were present and no mouse ever developed signs of acute illness. virus was recovered from lung, kidney, salivary gland, and liver, and virus excretion in urin ...19816116436
detection of hemorrhagic fever with renal syndrome (hfrs) virus in the lungs of bank voles clethrionomys glareolus and redbacked voles clethrionomys rutilus trapped in hfrs foci in the european part of u.s.s.r., and serodiagnosis of this infection in man.the antigen of hfrs virus was demonstrated by means of the indirect fluorescent antibody procedure in the lung tissue of bank and redbacked voles (clethrionomys glareolus, cl. rutilus) trapped in hfrs foci in the european part of ussr. this antigen has been used satisfactorily for serodiagnosis of hfrs in several european and asian regions of the ussr where hfrs had been found to be endemic.19816117265
korean hemorrhagic fever among rural residents in japan.naturally occurring infection with korean hemorrhagic fever virus among four families living in rural areas of northern and central japan was proved by the demonstration of antibody to the virus. furthermore, korean hemorrhagic fever virus antigen was detected in the lung tissues of wild rodents captured in the endemic area. these findings indicate the endemic presence of korean hemorrhagic fever virus on honshu island, japan.19816118386
[sbl test in the serologic diagnosis of epidemic nephropathy]. 19816119409
morphological identification of the agent of korean haemorrhagic fever (hantaan virus)as a member of the bunyaviridae.korean haemorrhagic fever (khf) (hantaan virus), a rodent-borne viral illness, is an important cause of human disease throughout much of asia and eastern europe. the agent responsible for khf has not yet been conclusively identified. plaque-purified khf virus was concentrated and then banded in a potassium tartrate gradient. material from the 1.17-1.19 g/ml band was examined by electron microscopy and particles with a morphology identical to that of the family bunyaviridae were found. the partic ...19826121225
taxonomic features of causative agent of haemorrhagic fever with renal syndrome. 19826121241
[laboratory diagnosis of hemorrhagic fever with renal syndrome (a review)]. 19806121396
[probable routes of the cycle in the nature of the causative agent of hemorrhagic fever with renal syndrome]. 19806121397
[demonstration of the virus of hemorrhagic fever with renal syndrome in the organs of rodents from natural foci of the infection in the ussr and the serodiagnosis of the diseases].an indirect fluorescent antibody test (ifat) and an enzyme-immunological test (elisa) were used for the detection of hfrs virus in organs of rodents from hfrs foci in the ussr. the virus was found in 115 out of 1120 bank voles, 9 out of 92 redbacked voles, and 2 field voles examined. spontaneous infection-rate of bank voles in population varied from 1.3 to 100% correlating with the epidemiological situation in foci. ifat and elisa were successfully used for serodiagnosis of hfrs. examinations of ...19816121425
serotypes of hfrs (haemorrhagic fever with renal syndrome) virus in east european and far eastern u.s.s.r. 19826122096
reovirus type 2 in strains of korean haemorrhagic fever virus. 19826122957
korean hemorrhagic fever. 19826124025
[the research work concerning the prevention and treatment of epidemic hemorrhagic fever in our entire nation (author's transl)]. 19826124371
[a case report which we considered to be the first case of field type korean hemorrhagic fever (khf) in japan and khf antigen in the field mice (author's transl)]. 19826125548
intracerebral inoculation of suckling mice with hantaan virus. 19826125672
hantaan virus is related to hemorrhagic fever with renal syndrome in norway. 19826125745
preliminary evidence that hantaan or a closely related virus is enzootic in domestic rodents. 19826125884
indirect immunofluorescence tests in korean hemorrhagic fever and epidemic (endemic) nephropathia: treatment at low ph for removal of 'nonspecific' fluorescence in tissues from immunocompetent hosts.a 'nonspecific' fluorescence probably caused by immunocomplexes appears in the lung tissue of immunocompetent hosts infected with korean hemorrhagic fever (khf) or epidemic nephropathy (en) viruses. indirect immunofluorescence tests with such khf- or en-infected tissue as antigen are unsatisfactory for the detection of low-titer antibody or the reading of titer endpoints. treatment of acetone-fixed lung sections at ph 3.0 followed by washing with 0.01 m phosphate-buffered saline and distilled wa ...19826126464
isolation of hantaan virus, the etiologic agent of korean hemorrhagic fever, from wild urban rats.urban rats captured in seoul and four nearby korean cities were found to have immunofluorescent antibodies reactive with hantaan virus, the etiologic agent of korean hemorrhagic fever (khf). serum antibodies were detected in 13% from 477 rattus norvegicus and 11% of 47 rattus rattus. hantaan viral antigen was found in pulmonary tissues of 42 animals, and hantaan virus was recovered from 23 rats, all but two of which were r. norvegicus. wistar rats were qualitatively much more sensitive than apod ...19826127366
laboratory-acquired infections with hantaan virus, the etiologic agent of korean hemorrhagic fever.clinically apparent infections with hantaan virus, the etiologic agent of korean hemorrhagic fever, occurred in nine persons exposed to the virus at the korea university virus institute (seoul) between 1971 and 1979. all were directly related to trapping of wild rodents or work with naturally or experimentally infected wild and laboratory rodents in a designated animal suite. all infections were acquired during the months of november-april, and none was associated with accidental parenteral inoc ...19826127367
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