TitleAbstractYear(sorted descending)
seroepidemiological study in a puumala virus outbreak area in south-east germany.puumala virus (puuv) is the cause of the majority of haemorrhagic fever with renal syndrome cases in germany. in 2004, a nephropathia epidemica outbreak was recorded in lower bavaria, south-east germany. for a seroepidemiological study in this region including the resident population at four locations (n = 178) and soldiers from one location (n = 208) indirect immunoglobulin m (igm) and immunoglobulin g (igg) enzyme-linked immunosorbent assays (elisas) and immunoblot tests based on a yeast-expre ...200919148676
relating increasing hantavirus incidences to the changing climate: the mast connection.nephropathia epidemica (ne), an emerging rodent-borne viral disease, has become the most important cause of infectious acute renal failure in belgium, with sharp increases in incidence occurring for more than a decade. bank voles are the rodent reservoir of the responsible hantavirus and are known to display cyclic population peaks. we tried to relate these peaks to the cyclic ne outbreaks observed since 1993. our hypothesis was that the ecological causal connection was the staple food source fo ...200919149870
plagues in the icu: a brief history of community-acquired epidemic and endemic transmissible infections leading to intensive care admission.the ability to diagnose and treat infectious diseases and handle infectious disease outbreaks continues to improve. for the most part, the major plagues of antiquity remain historical footnotes, yet, despite many advances, there is clear evidence that major pandemic illness is always just one outbreak away. in addition to the hiv pandemic, the smaller epidemic outbreaks of legionnaire's disease, hantavirus pulmonary syndrome, and severe acute respiratory syndrome, among many others, points out t ...200919268795
diagnostic aspects for epidemic hemorrhagic fever in legal medical autopsy: report of 2 cases and review.epidemic hemorrhagic fever (ehf) is known also as hemorrhagic fever with renal syndrome (hfrs). hantaviruses, a notorious cause of ehf is spreaded by rodents or biting insects, can cause epidemic hemorrhagic fever. this report describes two rare medico-legal autopsy cases died from epidemic hemorrhagic fever. the victims were two male individuals aged 34 years and 29 years. the medico-legal autopsies were requested for alleged medical negligence. in both cases, autopsy reveals lesions to kidneys ...200919269214
association between habitat and prevalence of hantavirus infections in bank voles (myodes glareolus) and wood mice (apodemus sylvaticus).in order to determine the habitat preferred by myodes (before clethrionomys) glareolus and the corresponding puumala hantavirus seroprevalence in those habitats, we captured rodents simultaneously in three significantly different habitats. we compared trapping success and presence of virus per habitat during an ongoing epidemic in order to test the hypothesis of a density-dependent seroprevalence. our study showed that bank vole population density, as well as puumala virus seroprevalence, were h ...200919271997
drug targets in infections with other emerging viruses: influenza viruses, metapneumovirus and hantaviruses.among emergent and re-emergent viral infections, influenza, hemorrhagic fevers, including hantaviruses, constitute one of the major threats to human beings. advances in immunopathogenesis of these viral infections have improved initial supportive treatments and led to recognition and adoption of several useful antiviral agents. this review focuses on therapeutic and preventive aspects of these viral infections, and evaluates old and new promising agents are in the pipeline of pharmaceutical comp ...200919275703
andes virus antigens are shed in urine of patients with acute hantavirus cardiopulmonary syndrome.hantavirus cardiopulmonary syndrome (hcps) is a highly pathogenic emerging disease (40% case fatality rate) caused by new world hantaviruses. hantavirus infections are transmitted to humans mainly by inhalation of virus-contaminated aerosol particles of rodent excreta and secretions. at present, there are no antiviral drugs or immunotherapeutic agents available for the treatment of hantaviral infection, and the survival rates for infected patients hinge largely on early virus recognition and hos ...200919279096
[anterior pituitary lobe atrophy as late complication of hemorrhagic fever with renal syndrome].hemorrhagic fever with renal syndrome (hfrs) is acute infective multisystemic disease followed by febrility, hemorrhages and acute renal insufficiency. bleeding in the anterior pituitary lobe leading to tissue necrosis occurs in acute stage of severe clinical forms of hfrs, while atrophy of the anterior pituitary lobe with diminution of the gland function occurs after recovery stage.200919281130
platelet glycoprotein iib/iiia (hpa-1 and hpa-3) polymorphisms in patients with hemorrhagic fever with renal syndrome.this study investigated the relationship between human platelet alloantigen (hpa) polymorphisms of glycoprotein iib/iiia and hemorrhagic fever with renal syndrome (hfrs). hpa-1 and hpa-3 genotyping was performed with allele-specific primer polymerase chain reaction in 104 patients with hfrs and 100 normal individuals as controls. the relationships between gene polymorphisms of hpas and hfrs and the disease severity were analyzed. the results indicated no significant difference in hpa-1 genotype ...200919286442
a novel cardiotropic murine adenovirus representing a distinct species of mastadenoviruses.during cell culture isolation experiments to recover dobrava hantavirus from a suspension of liver from a striped field mouse (apodemus agrarius), an unknown virus was coisolated. atypically for hantaviruses, it had extensive cytopathic effects. using a random pcr approach, it was identified as a novel murine adenovirus, madv-3 (for madv type 3). a plaque-purified virus clone was prepared and further characterized. the complete genome sequence of madv-3 was determined to be 30,570 bp in length. ...200919297486
hantavirus pulmonary syndrome, central plateau, southeastern, and southern brazil.hantavirus pulmonary syndrome (hps) is an increasing health problem in brazil because of encroachment of sprawling urban, agricultural, and cattle-raising areas into habitats of subfamily sigmodontinae rodents, which serve as hantavirus reservoirs. from 1993 through june 2007, a total of 884 cases of hps were reported in brazil (case-fatality rate 39%). to better understand this emerging disease, we collected 89 human serum samples and 68 rodent lung samples containing antibodies to hantavirus f ...200919331732
bunyavirus n: eif4f surrogate and cap-guardian.hantaviruses comprise a genus of the bunyavirus family of viruses. viruses of this family, along with the arenaviruses, and the orthomyxoviruses, including influenza, contain a negative sense, segmented rna genome. viral nucleocapsid proteins play a well-established role in the formation of intracellular and virion-associated nucleocapsids that harbor and shield viral genomic rna. however, recent observations indicate that hantavirus nucleocapsid protein (n) has additional unexpected biological ...200919342890
predicting the risk of hantavirus infection in beijing, people's republic of understand the spatial distribution of hantavirus infections across landscapes and its influencing environment factors in beijing, people's republic of china, rodents captured in this region were examined for hantavirus by reverse transcription-polymerase chain reaction. a total of 1,639 rodents were trapped at 86 randomly selected sites. the overall infection rate for hantavirus was 7.14% in the rodents. multivariate logistic regression analysis indicated that the natural infection rates for ...200919346399
[purpura fulminans, cerebral infarcts and multiorganic dysfunction due to hantavirus infection. review and case report].multiorganic failure is a rare manifestation of hantavirus infection but it should be included among differential diagnoses of multiorganic failure in the pediatric age. currently, there is no effective therapy for this infection. a high suspicion index and early referral to a pediatric intensive care unit with extracorporeal membrane oxygenation may lead to a favourable impact in the outcome. we report a fatal case of hantavirus infection in a 9-year-old girl with a severe disease refractory to ...200919350147
[diagnosis and treatment of cardiopulmonary hantavirus syndrome: chile-2007]. 200919350164
characterization of imjin virus, a newly isolated hantavirus from the ussuri white-toothed shrew (crocidura lasiura).until recently, the single known exception to the rodent-hantavirus association was thottapalayam virus (tpmv), a long-unclassified virus isolated from the asian house shrew (suncus murinus). robust gene amplification techniques have now uncovered several genetically distinct hantaviruses from shrews in widely separated geographic regions. here, we report the characterization of a newly identified hantavirus, designated imjin virus (mjnv), isolated from the lung tissues of ussuri white-toothed s ...200919357167
analysis of puumala hantavirus in a bank vole population in northern finland: evidence for co-circulation of two genetic lineages and frequent reassortment between this study, for the first time, two distinct genetic lineages of puumala virus (puuv) were found within a small sampling area and within a single host genetic lineage (ural mtdna) at pallasjärvi, northern finland. lung tissue samples of 171 bank voles (myodes glareolus) trapped in september 1998 were screened for the presence of puuv nucleocapsid antigen and 25 were found to be positive. partial sequences of the puuv small (s), medium (m) and large (l) genome segments were recovered from thes ...200919386780
hanta virus infection during dengue virus infection outbreak in investigate which recent infection could have caused the present dengue-like symptoms, in adult patients clinically fulfilling the who criteria for dengue, in which serologically were not confirmed for dengue virus infections.200919390126
tubular proteinuria and glomerular filtration 6 years after puumala hantavirus-induced acute interstitial nephritis.we previously found increased urinary protein excretion, glomerular filtration rate (gfr) and blood pressure in a retrospective analysis of patients with previous nephropathia epidemica (ne). here, we evaluated the long-term outcome after ne in a prospectively recruited patient group.200919390211
a proposal for new criteria for the classification of hantaviruses, based on s and m segment protein sequences.hantaviruses, members of the family bunyaviridae, are the causative agents of hemorrhagic fever with renal syndrome and hantavirus cardiopulmonary syndrome. hantaviruses are currently demarcated into species based on the guidelines provided by the international committee on taxonomy of viruses (ictv). these guidelines however, are often ignored by the descriptors of novel hantaviruses. with this study we attempted to refine the second ictv guideline for hantavirus species demarcation by phylogen ...200919393771
host switch during evolution of a genetically distinct hantavirus in the american shrew mole (neurotrichus gibbsii).a genetically distinct hantavirus, designated oxbow virus (oxbv), was detected in tissues of an american shrew mole (neurotrichus gibbsii), captured in gresham, oregon, in september 2003. pairwise analysis of full-length s- and m- and partial l-segment nucleotide and amino acid sequences of oxbv indicated low sequence similarity with rodent-borne hantaviruses. phylogenetic analyses using maximum-likelihood and bayesian methods, and host-parasite evolutionary comparisons, showed that oxbv and asa ...200919394994
evidence of circulation of laguna negra-like hantavirus in the central west of brazil: case report.hantavirus pulmonary syndrome has been reported with increasing frequency in some brazilian regions, but information about viral genetic identification is still limited. recently, the state of mato grosso, in the legal amazon of brazil, experienced a growing number of hantavirus pulmonary syndrome (hps) cases but the genetic characterization of the causative hantavirus is still missing.200919395308
seasonal variation in sin nombre virus infections in deer mice: preliminary results.the proportion of deer mice (peromyscus maniculatus) with recently acquired sin nombre virus (snv) infections is an indicator of epizootic intensity and may be key in predicting outbreaks of hantavirus cardio-pulmonary syndrome in humans. we investigated whether incidence of recent infections was related to season, sex, reproductive status, or habitat disturbance. in may and september, 2006, we sampled 912 deer mice at six sites in utah. we determined snv antibody prevalence and estimated the nu ...200919395752
the hantaviral load in tissues of naturally infected rodents.hantaviruses cause a lifelong and asymptomatic infection in naturally infected hosts as well as in experimentally infected rodents. understanding the ecology and pathogenesis of hantaviruses requires an interdisciplinary research approach, which links laboratory experiments with results gained from field studies. although several studies report hantavirus persistence and tissue infection patterns for experimentally infected rodents, field data is very limited. for this reason, the aim of our stu ...200919397875
adenovirus vectors expressing hantavirus proteins protect hamsters against lethal challenge with andes virus.hantaviruses infect humans following aerosolization from rodent feces and urine, producing hemorrhagic fever with renal syndrome and hantavirus pulmonary syndrome. due to the high rates of mortality and lack of therapies, vaccines are urgently needed. nonreplicating adenovirus (ad) vectors that express andes hantavirus (andv) nucleocapsid protein (adn) or glycoproteins (adg(n) and adg(c)) were constructed. ad vectors were tested for their ability to protect syrian hamsters from a lethal andv inf ...200919403663
replication reduction neutralization test, a quantitative rt-pcr-based technique for the detection of neutralizing hantavirus antibodies.hantaviruses, which are mainly rodent-borne viruses, cause hemorrhagic fever with renal syndrome in the old world, and hantavirus pulmonary syndrome in the new world. a neutralization test based on quantitative rt-pcr, the replication reduction neutralization test (rrnt), was developed for efficient detection of hantavirus-neutralizing antibodies. the effectiveness of the rrnt was evaluated by examining several hantaviruses and hantavirus-specific convalescent human serum samples. all convalesce ...200919406158
first molecular evidence of tula hantavirus in microtus voles in slovenia.different microtus species, present in a worldwide range habitat populating north america, europe, asia, and few other species have been recognized previously as a hantavirus reservoir. tula hantavirus was first reported in microtus arvalis and microtus rossiaemeridionalis from central russia and later discovered in several european countries. using molecular techniques we have demonstrated the presence of tula hantavirus in three different microtus species in slovenia. phylogenetic analyses of ...200919410611
experimental evidence for reduced rodent diversity causing increased hantavirus prevalence.emerging and re-emerging infectious diseases have become a major global environmental problem with important public health, economic, and political consequences. the etiologic agents of most emerging infectious diseases are zoonotic, and anthropogenic environmental changes that affect wildlife communities are increasingly implicated in disease emergence and spread. although increased disease incidence has been correlated with biodiversity loss for several zoonoses, experimental tests in these sy ...200919421313
is hantavirus important to practitioners? 200919434032
hantavirus pulmonary syndrome: a concise clinical 1978, hantaviruses were first described as the etiological agent of hemorrhagic fever with renal syndrome (hfrs) in korea. since then, numerous related, enveloped, negative-stranded rna viruses have been identified, forming the genus hantavirus within the family bunyaviridae. these pathogens are distributed worldwide and thus can be classified, on the basis of phylogenetic origins, into old world viruses or new world viruses (ie north, central, and south america). similarly, these viruses cau ...200919434035
phylogenetic characterization of hantaviruses from wild rodents and hantavirus pulmonary syndrome cases in the state of parana (southern brazil).over 1,100 cases of hantavirus pulmonary syndrome (hps) have occurred in brazil since 1993, but little is known about brazilian hantaviruses, and many of their rodent hosts remain unknown. the araucaria hantavirus (arauv) was described recently from hps patients from paraná, in southern brazil, but its host could not be identified. in this study, rodents were captured from regions with high hps prevalence to address this issue. arauv rna was detected in three distantly related rodent species: ol ...200919439554
new genetic lineage of tula hantavirus in microtus arvalis obscurus in eastern kazakhstan.genomic sequences of tula (tulv) hantavirus were recovered from tissue samples of european common voles microtus arvalis (subspecies obscurus) captured in kazakhstan, central asia. phylogenetic analysis of the s genomic segment of kazakh tulv strains showed that they form distinct, well supported genetic lineage and share a more ancient common ancestor with two russian lineages of tulv. the deduced sequence of the nucleocapsid (n) protein of kazakh tulv strains carried specific amino acid signat ...200819440462
[the molecular epidemiologic investigation of hantavirus carried by rodent hosts in wenzhou, zhejiang province].to study the epidemiological features of hantavirus in rodents in wenzhou, zhejiang province.200819173853
[the dynamics of t lymphocyte subsets in hemorrhagic fever with renal syndrome].to investigate the t cell subsets changes in hemorrhagic fever with renal syndrome (hfrs) patients.200819080298
expression of l protein of hantaan virus 84fli strain and its application for recovery of minigenomes.hantaan virus (htnv) is an old world hantavirus associated with hemorrhagic fever with renal syndrome (hfrs). to visualize the localization of the l protein of htnv strain 84fli within cells, a fusion protein composed of enhanced green fluorescent protein and l protein, egfp-l, was expressed in vero cells. the 273 kda expressed fusion protein of egfp-l localized in the perinuclear region. we also described the development of a reverse genetics system for htnv strain 84fli. the rna polymerase i ( ...200819133012
[investigation of modes of hantavirus infection transmission from rodents to humans].hemorrhagic fever with renal syndrome (hfrs) is a focal zoonosis of viral etiology that appears in many countries of the world. investigation demonstrated that for epidemic of hfrs is necessary to have intensity of epizootic process. the increased number of mice-like rodents in enzootic foci of hfrs is the most important factor. this investigation pointed out to high risk of direct contact with rodents secrets, from visible urine and feces traces of rodents that are present in large number of pa ...200819145808
the effect of habitat fragmentation and species diversity loss on hantavirus prevalence in panama.habitat fragmentation and diversity loss due to increased conversion of natural habitats to agricultural uses influence the distribution and abundance of wildlife species and thus may change the ecology of pathogen transmission. we used hantaviruses in panama as a research model to determine whether anthropogenic environmental change is associated with changes in the dynamics of viral transmission. specifically, we wanted to determine whether hantavirus infection was correlated with spatial attr ...200819120179
recent discoveries of new hantaviruses widen their range and question their origins.hantaviruses belong to the bunyaviridae family. while usually hosted by wild mammals, they are potentially pathogenic for humans, and several serologically distinct groups associated with different syndromes have been identified. yet, investigations have mostly been conducted where human infections by hantaviruses constitute a real and well-identified public health problem, i.e., the holarctic and neotropical areas. some hantaviruses have also been described from a suncus murinus in india and a ...200819120180
[detection of anti-hantavirus antibodies in forest workers in the north-east of poland].to estimate exposition to hantaviruses in a high-risk population (forest workers) in the north-east of poland.200819108515
[molecular epidemiological study on the host and role of the hantavirus and orientia tsutsugamushi in the same epidemic area].to investigate whether hantavirus (hv) and orientia tsutsugamushi ( ot) can naturally infect and coexist in their host and role.200819115620
surveillance of vector-borne diseases in germany: trends and challenges in the view of disease emergence and climate change.the changing epidemiology of vector-borne diseases represents a growing threat to human health. contemporary surveillance systems have to adapt to these changes. we describe temporal trends and geographic origins of vector-borne diseases in germany with regard to strengths of existing disease surveillance and to areas marked for improvement. we focused on hantavirus infection (endemic in germany), chikungunya fever (recently emerging in europe) and dengue fever (imported from tropical regions), ...200819030882
network "rodent-borne pathogens" in germany: longitudinal studies on the geographical distribution and prevalence of hantavirus infections.hantavirus infections are known in germany since the 1980s. while the overall antibody prevalence against hantaviruses in the general human population was estimated to be about 1-2%, an average of 100-200 clinical cases are recorded annually. in the years 2005 and 2007 in particular, a large increase of the number of human hantavirus infections in germany was observed. the most affected regions were located in the federal states of baden-wuerttemberg, bavaria, north rhine westphalia, and lower s ...200819030894
emergence of hantavirus in south germany: rodents, climate and human infections.human hantavirus (serotype puumala) infections are prevalent throughout europe. the bank vole is the main reservoir of the puumala virus (puuv). between 2001 and 2006, the annual incidences in germany ranged from 0.1 to 0.5 per 100,000 inhabitants. about half of the cases were reported from the state of baden-württemberg (bw) in southwest germany. in 2007, 1,089 puuv infections were reported from bw. this reflects an incidence of 10.1:100,000, which is more than 11 times higher than the mean inc ...200819030895
[hantavirus mucosal vaccine through different mucosal with heat-labile enterotoxin b subunit as adjuvants].to evaluation the effect of different mucosal vaccination pathway on hantavirus with the recombinant e. coli heat-labile enterotoxin b subunit (rltb) as adjuvant.200819031694
[the reactivity of sera from hemorrhagic fever in patients with renal syndromes to the recombination nucleotide proteins from european hantaviruses in hubei province].five yeast-expressed recombination nucleotide proteins of european hantaviruses were prepared as coated antigens to detect hantavirus-specific antibodies in sera from hemorrhagic fever with renal syndromes (hfrs) in hubei province, through elisa assay. the relativity among hantaviruses prevailing in different areas was investigated.200819040041
antiviral effect of human saliva against hantavirus.hemorrhagic fever with renal syndrome (hfrs) and hantavirus pulmonary syndrome are zoonotic diseases caused by rodent borne hantaviruses. transmission to humans occurs usually by inhalation of aerozolized virus-contaminated rodent excreta. although human-to-human transmission of andes hantavirus has been observed, the mode of transmission is currently not known. saliva from puumala hantavirus (puuv)-infected patients was shown recently to contain viral rna. to test if human saliva interferes wit ...200819040288
immunological mechanisms mediating hantavirus persistence in rodent reservoirs.hantaviruses, similar to several emerging zoonotic viruses, persistently infect their natural reservoir hosts, without causing overt signs of disease. spillover to incidental human hosts results in morbidity and mortality mediated by excessive proinflammatory and cellular immune responses. the mechanisms mediating the persistence of hantaviruses and the absence of clinical symptoms in rodent reservoirs are only starting to be uncovered. recent studies indicate that during hantavirus infection, p ...200819043585
sin nombre virus infection in deer mice, channel islands, california. 200819046540
storage of cellular 5' mrna caps in p bodies for viral cap-snatching.the minus strand and ambisense segmented rna viruses include multiple important human pathogens and are divided into three families, the orthomyxoviridae, the bunyaviridae, and the arenaviridae. these viruses all initiate viral transcription through the process of "cap-snatching," which involves the acquisition of capped 5' oligonucleotides from cellular mrna. hantaviruses are emerging pathogenic viruses of the bunyaviridae family that replicate in the cytoplasm of infected cells. cellular mrnas ...200819047634
[increased incidence of hantavirus infection in germany and austria]. 200818937155
genetic immunization with hantavirus vaccine combining expression of g2 glycoprotein and fused this research, we developed a novel chimeric htnv-il-2-g2 dna vaccine plasmid by genetically linking il-2 gene to the g2 segment dna and tested whether it could be a candidate vaccine. chimeric gene was first expressed in eukaryotic expression system pcdna3.1 (+). the htnv-il-2-g2 expressed a 72 kda fusion protein in cos-7 cells. meanwhile, the fusion protein kept the activity of its parental proteins. furthermore, balb/c mice were vaccinated by the chimeric gene. elisa, cell microculture neu ...200818940009
roles for the recycling endosome, rab8, and rab11 in hantavirus release from epithelial cells.hantavirus structural proteins are believed to localize to intracellular membranes often identified as golgi membranes, in virus-infected cells. after virus budding into the golgi luminal space, virus-containing vesicles are transported to the plasma membrane via trafficking pathways that are not well defined. using the new world hantavirus, andes virus, we have investigated the role of various rab proteins in the release of hantavirus particles from infected cells. rabs 8 and 11 were found to c ...200818951604
the role of heterogeneity in the persistence and prevalence of sin nombre virus in deer mice.many diseases persist at a relatively low prevalence, seemingly close to extinction. for a chronic disease in a homogeneous population, reducing the transmission rate by a fraction proportional to the prevalence would be sufficient to eradicate the disease. this study examines how higher prevalence of the sin nombre virus in male deer mice (peromyscus maniculatus) might contribute to disease persistence. analyzing data from over 2,000 individual mice captured in 19 sites over 4 years, we found p ...200818959490
a protein that replaces the entire cellular eif4f complex.the eif4f cap-binding complex mediates the initiation of cellular mrna translation. eif4f is composed of eif4e, which binds to the mrna cap, eif4g, which indirectly links the mrna cap with the 43s pre-initiation complex, and eif4a, which is a helicase necessary for initiation. viral nucleocapsid proteins (n) function in both genome replication and rna encapsidation. surprisingly, we find that hantavirus n has multiple intrinsic activities that mimic and substitute for each of the three peptides ...200818971945
genomic organization and phylogenetic utility of deer mouse (peromyscus maniculatus) lymphotoxin-alpha and lymphotoxin-beta.deer mice (peromyscus maniculatus) are among the most common mammals in north america and are important reservoirs of several human pathogens, including sin nombre hantavirus (snv). snv can establish a life-long apathogenic infection in deer mice, which can shed virus in excrement for transmission to humans. patients that die from hantavirus cardiopulmonary syndrome (hcps) have been found to express several proinflammatory cytokines, including lymphotoxin (lt), in the lungs. it is thought that t ...200818976466
first genetic detection of tula hantavirus in wild rodents in the netherlands. 200818977537
hantavirus antibodies in rodents and human cases with pulmonary syndrome, rio negro, río negro province, argentina, human cases of hantavirus pulmonary syndrome (hps) appeared in the region of subantarctic forests. the andes virus (andv) has been identified in the region both in oligoryzomys longicaudatus rodents and in humans, with the main transmission being from rodents to humans but also showing the possibility of human to human transmission. between 1996 and 2004, in 40 campaigns, 29.960 night-traps for capturing live rodents were set up. blood samples were obtained from ...200818977707
hantavirus cardiopulmonary syndrome: implications for transport management and care.a 14-year-old boy with cardiorespiratory failure was referred for air medical transport. the complexity of care during air medical transport and subsequent diagnosis of hantavirus warranted a post hoc review of the literature to establish optimal transport management criteria.200818992690
natural host relationships and genetic diversity of rodent-associated hantaviruses in southeastern brazil.hantaviruses are rodent-borne rna viruses that have caused hantavirus cardiopulmonary syndrome in several brazilian regions. in the present study, geographical distribution, seroprevalence, natural host range, and phylogenetic relations of rodent-associated hantaviruses collected from seven counties of southeastern brazil were evaluated.200819001829
corticosteroids modulate seoul virus infection, regulatory t-cell responses and matrix metalloprotease 9 expression in male, but not female, norway rats.human hantaviral disease is mediated by excessive proinflammatory and cd8+ t-cell responses, which can be alleviated by administration of corticosteroids. in contrast with humans, male rats that are infected with their species-specific hantavirus, seoul virus (seov), have reduced proinflammatory and elevated regulatory t-cell responses in tissues where virus persists. to determine the effects of glucocorticoids on seov persistence and immune responses during infection, male and female norway rat ...200818931068
hantaviruses and tnf-alpha act synergistically to induce erk1/2 inactivation in vero e6 cells.we have previously reported that the apathogenic tula hantavirus induces apoptosis in vero e6 epithelial cells. to assess the molecular mechanisms behind the induced apoptosis we studied the effects of hantavirus infection on cellular signaling pathways which promote cell survival. we previously also observed that the tula virus-induced cell death process is augmented by external tnf-alpha. since tnf-alpha is involved in the pathogenesis of hantavirus-caused hemorrhagic fever with renal syndrome ...200818822184
a hantavirus causing hemorrhagic fever with renal syndrome requires gc1qr/p32 for efficient cell binding and infection.hantaan virus (htnv) is a pathogenic hantavirus that causes hemorrhagic fever with renal syndrome (hfrs). htnv infection is mediated by alpha v beta3 integrin. we used protein blots of vero e6 cell homogenates to demonstrate that radiolabeled htnv virions bind to gc1qr/p32, the acidic 32-kda protein known as the receptor for the globular head domain of complement c1q. rnai-mediated suppression of gc1qr/p32 markedly reduced htnv binding and infection in human lung epithelial a549 cells. conversel ...200818834607
first detection of tula hantaviruses in microtus arvalis voles in hungary.tula hantavirus (tulv) is a member of the genus hantavirus, family bunyaviridae and is mainly carried by the european common vole (microtus arvalis). in order to detect tulv, we tested microtus arvalis (mar) and microtus subterraneus (msu) voles captured in two different locations of the southern transdanubian region of hungary. the viral genome was detectable in 37% of the tested mar voles but, interestingly, was absent in all msu. phylogenetic analysis performed with a partial coding sequence ...200818836679
hantavirus-induced immunity in rodent reservoirs and humans.summary: hantaviruses are predominantly rodent-borne pathogens, although recently novel shrew-associated hantaviruses were found. within natural reservoir hosts, hantairuses do not cause obvious pathogenetic effects; transmission to humans, however, can lead to hemorrhagic fever with renal syndrome or hantavirus cardiopulmonary syndrome, depending on the virus species involved. this review is focussed on the recent knowledge on hantavirus-induced immune responses in rodent reservoirs and humans ...200818837782
[study on the molecular epidemiology of hantavirus carried by rodent hosts in cixi, zhejiang province].to collect more data on the epidemiology of hantavirus in rodents in cixi, zhejiang province.200818843995
[study on the molecular characteristic of natural infection of rodents with hantaviruses in shenzhen city].in order to investigate hantavirus (hv) infection of captured rodents and to understand the genotypes and the molecular characteristic of hantaviruses in shenzhen.200818844081
molecular phylogeny of a newfound hantavirus in the japanese shrew mole (urotrichus talpoides).recent molecular evidence of genetically distinct hantaviruses in shrews, captured in widely separated geographical regions, corroborates decades-old reports of hantavirus antigens in shrew tissues. apart from challenging the conventional view that rodents are the principal reservoir hosts, the recently identified soricid-borne hantaviruses raise the possibility that other soricomorphs, notably talpids, similarly harbor hantaviruses. in analyzing rna extracts from lung tissues of the japanese sh ...200818854415
case report: t-cell responses during clearance of andes virus from blood cells 2 months after severe hantavirus cardiopulmonary syndrome.hantavirus cardiopulmonary syndrome (hcps) due to andes virus (andv) is endemic in chile and argentina and currently demonstrates a case-fatality rate of 37% in humans. by contrast to the chronically infected rodents, it is believed that andv in humans is cleared during the acute phase. moreover, to date, both magnitude and quality of human t-cell responses during andv infection and clearance are unknown. using ifn-gamma and granzyme b elispot assays as well as flow cytometry, we prospectively s ...200818814258
hemorrhagic fever with renal syndrome in montenegro.the objective of the study was to analyze the epidemiological features of hemorrhagic fever with renal syndrome (hfrs) in montenegro. the study included 169 cases of hfrs diagnosed in the period between 1995 and 2005 according to the clinical symptoms and serological confirmation. for the analysis of the demographic characteristics of the cases, as well as of the chronological and topographical features of the disease, a descriptive epidemiological method was employed. the average incidence rate ...200818806348
nmr structure of the n-terminal coiled coil domain of the andes hantavirus nucleocapsid protein.the hantaviruses are emerging infectious viruses that in humans can cause a cardiopulmonary syndrome or a hemorrhagic fever with renal syndrome. the nucleocapsid (n) is the most abundant viral protein, and during viral assembly, the n protein forms trimers and packages the viral rna genome. here, we report the nmr structure of the n-terminal domain (residues 1-74, called n1-74) of the andes hantavirus n protein. n1-74 forms two long helices (alpha1 and alpha2) that intertwine into a coiled coil ...200818687679
role of mixed th1 and th2 serum cytokines on pathogenesis and prognosis of hantavirus pulmonary syndrome.the hantavirus pulmonary syndrome (hps) is an emerging syndrome in the americas. the disease results from intense immune activation and changes in vascular permeability. the aim of this study was to determine the profile of serum cytokines in hps patients looking for correlation with the clinical parameters, severity and outcome of illness. studying 21 hps patients, we found that il-6 may have an important role in the pathogenesis of hps, being associated with fatal outcome. our results also sup ...200818606242
lack of vertical transmission of hantaan virus from persistently infected dam to progeny in laboratory is unclear how the hantaviruses are transferred from infected to uninfected rodents. we studied the status of persistently infected laboratory mice and examined the frequency of viral transmission to their offspring. expression of hantaan virus nucleocapsid protein was detected in the lungs of persistently infected dams. none of the progeny displayed viral antigen, although they were strongly positive for igg antibodies against hantavirus. there was neither hantavirus rna nor virus-specific i ...200818612586
the ny-1 hantavirus gn cytoplasmic tail coprecipitates traf3 and inhibits cellular interferon responses by disrupting tbk1-traf3 complex formation.pathogenic hantaviruses replicate within human endothelial cells and cause two diseases, hemorrhagic fever with renal syndrome and hantavirus pulmonary syndrome. in order to replicate in endothelial cells pathogenic hantaviruses inhibit the early induction of beta interferon (ifn-beta). expression of the cytoplasmic tail of the pathogenic ny-1 hantavirus gn protein is sufficient to inhibit rig-i- and tbk1-directed ifn responses. the formation of tbk1-traf3 complexes directs irf-3 phosphorylation ...200818614628
a serosurvey for hantavirus infection in wild rodents from the states of rio de janeiro and pernambuco, brazil.sera from 269 rodents obtained during the routine surveillance operations in plague areas of rio de janeiro and pernambuco states, brazil were tested by elisa for specific igg antibodies against a recombinant nucleocapsid (n) protein of araraquara hantavirus. elisa-positive sera were submitted to reverse transcriptase-polymerase chain reaction (rt-pcr) for amplification of the virus genome and later sequencing for identification of the viral variant. the samples from the state of pernambuco were ...200818619568
cross-reactive and serospecific epitopes of nucleocapsid proteins of three hantaviruses: prospects for new diagnostic tools.the diagnosis of infectious diseases is sometimes difficult because of extensive immunological cross-reactivity between related viral antigens. on the path of constructing sero-specific antigens, we have identified residues involved in sero-specific and cross-reactive recognition of the nucleocapsid proteins (nps) of puumala virus (puuv), seoul virus (seov), and sin nombre virus (snv) using serum samples from 17 nephropathia epidemica patients. the mapping was performed by enzyme-linked immunoso ...200818620010
genetic analysis of hantaviruses carried by reed voles microtus fortis in gain more insights into the epidemiology of hantaviruses in china, the hantaviral s, m and l segment sequences were recovered from two microtus fortis captured in shenyang and four m. fortis trapped in yuanjiang in china. genetic analysis revealed that shenyang sequences are closely related to the sequences of fusong strains of vladivostok virus (vlav). interestingly, the complete s segment and partial l segment sequences from yuanjiang were quite distinct from those of shenyang and fusong st ...200818644410
hantavirus nephropathy.pathogenic rodent-borne hantaviruses cause in humans generalized infections that involve the peripheral vascular bed and severely affect their permeability. we describe a 30-yr-old male patient with clinical symptoms characterizing five conventional phases of hemorrhagic fever with renal syndrome after an uncommonly severe hantavirus infection with the puumala strain. renal biopsy in this situation typically demonstrates acute hemorrhagic interstitial nephritis, particularly pronounced in the ou ...200818650483
[the hantaviruses causing hemorrhagic fever with renal syndrome and pulmonary syndrome].the goal of this review is to provide basic information on hantaviruses as causative agents of hemorrhagic fever with renal syndrome (hfrs) and hantavirus pulmonary syndrome (hps), two zoonotic diseases widely distributed in asia/europe, and the american continent, respectively. hantaviruses are rodent-borne and transmitted to humans by direct contact with infected rodents or their secretions (urine, feces and saliva). both, hfrs and hps share some clinical aspects, however, hemorrhage and renal ...200818670726
bench-to-bedside review: rare and common viral infections in the intensive care unit--linking pathophysiology to clinical presentation.viral infections are common causes of respiratory tract disease in the outpatient setting but much less common in the intensive care unit. however, a finite number of viral agents cause respiratory tract disease in the intensive care unit. some viruses, such as influenza, respiratory syncytial virus (rsv), cytomegalovirus (cmv), and varicella-zoster virus (vzv), are relatively common. others, such as adenovirus, severe acute respiratory syndrome (sars)-coronavirus, hantavirus, and the viral hemo ...200818671826
[first case of nephropathia epidemica acquired in switzerland].a 43 year healthy old man complains of fever with abdominal pain, vomiting, diarrhoea, followed by the development of thrombocytopenia and acute renal failure. the laboratory tests show the presence of hantavirus specific igm and igg which is confirmed by a specific test revealing puumala serotype as responsible. the patient received a symptomatic treatment with a favourable evolution allowing discharge about ten days after the beginning of symptoms. hantavirus are transmitted by rodents, and th ...200818672548
puumala hantavirus excretion kinetics in bank voles (myodes glareolus).puumala hantavirus is present in bank voles (myodes glareolus) and is believed to be spread mainly by contaminated excretions. in this study, we subcutaneously inoculated 10 bank voles with puumala virus and sampled excretions until day 133 postinfection. levels of shed viral rna peaked within 11-28, 14-21, and 11-28 days postinfection for saliva, urine, and feces, respectively. the latest detection of viral rna was 84, 44, and 44 days postinfection in saliva, urine, and feces, respectively. in ...200818680643
[zoonoses transmitted by mouse and rat maintained as laboratory or pet animals].large numbers of mice (mus spp.) and rats (rattus spp.) are maintained for scientific reasons and as pet animals in germany. while laboratory animals are monitored for pathogenic agents, the hygienic status of pet animals is usually completely unknown. despite great efforts, zoonotic infections were reported even in laboratory settings, e.g. with hantavirus (seoul virus), streptobacillus moniliformis, and trichphyton mentagrophytes. however, in current reports, zoonotic infections were transmitt ...200818712260
pathology of puumala hantavirus infection in macaques.hantaviruses are globally important human pathogens that cause hemorrhagic fever with renal syndrome and hantavirus pulmonary syndrome. capillary leakage is central to hantaviral diseases, but how it develops, has remained unknown. it has been hypothesized that the pathogenesis of hantavirus infection would be a complex interplay between direct viral effects and immunopathological mechanisms. both of these were studied in the so far best model of mild hemorrhagic fever with renal syndrome, i.e. ...200818716663
expression of a hantavirus n protein and its efficacy as antigen in immune assays.hantavirus cardiopulmonary syndrome (hcps) has been recognized as an important public heath problem. five hantaviruses associated with hcps are currently known in brazil: juquitiba, araraquara, laguna negra-like, castelo dos sonhos, and anajatuba viruses. the laboratory diagnosis of hcps is routinely carried out by the detection of anti-hantavirus igm and/or igg antibodies. the present study describes the expression of the n protein of a hantavirus detected in the blood sample of an hcps patient ...200818719741
oligomerization of hantaviral nucleocapsid protein: charged residues in the n-terminal coiled-coil domain contribute to intermolecular interactions.the nucleocapsid (n) protein of hantaviruses (family bunyaviridae) is the most abundant component of the virion; it encapsidates genomic rna segments and participates in viral genome transcription and replication, as well as in virus assembly. during rna encapsidation, the n protein forms intermediate trimers and then oligomers via 'head-to-head, tail-to-tail' interactions. in previous work, using tula hantavirus (tulv) n protein as a model, it was demonstrated that an intact coiled-coil structu ...200818753226
vector-borne diseases threaten europe. 200818754168
[immunogenicity of n-protein of puumala hantavirus for noninbred mice in the intramuscular administration of its gene dna].the outbreaks of hantavirus infections in some regions of the russian federation in some years involve considerable material and social losses. in this connection, the designing of the most effective types of vaccines is an urgent task. the authors have created plasmid constructions containing the gene of hemorrhagic fever with renal syndrome virus, with whose intramuscular injection there is a specific immune response and plasmid dna is detectable in the adjacent tissues within a month after in ...200818756816
juquitiba-like hantavirus from 2 nonrelated rodent species, uruguay.serologic and genetic analyses indicate that a juquitiba-like hantavirus circulates in maldonado, uruguay. this virus is carried by 2 rodent species, oligoryzomys nigripes and oxymycterus nasutus. the same hantavirus in 2 nonrelated species can be explained by a spillover infection or a host-switching event.200818760017
situation of hantavirus infections and haemorrhagic fever with renal syndrome in european countries as of december 2006.hantavirus infections are widely distributed in europe with the exception of the far north and the mediterranean regions. the underlying causes of varying epidemiological patterns differ among regions: in western and central europe epidemics of haemorrhagic fever with renal syndrome (hfrs) caused by hantavirus infections follow mast years with increased seed production by oak and beech trees followed by increased rodent reproduction. in the northern regions, hantavirus infections and hfrs epidem ...200818761927
[nephropathia epidemica as the result of a puumala virus infection in a pregnant patient].a 29-year-old woman in the early stage of pregnancy ( approximately 6 (th) week) presented in our hospital with influenza-like symptoms of fever, headache, aching limbs and tenderness over the costovertebral angle. acute oliguric renal failure occurred within a few days.200818770481
heat shock protein 70 fused to or complexed with hantavirus nucleocapsid protein significantly enhances specific humoral and cellular immune responses in c57bl/6 mice.heat shock proteins (hsps) are known to act as an effective molecular adjuvant to enhance the induction of antigen peptide-specific cellular immunity, when coupled with the antigen or peptide. hantaan virus (htnv) nucleocapsid protein (np) is relatively conserved among hantaviruses and highly immunogenic in both animals and humans. to analyze the influence of hsp70 on np vaccine potency, and evaluate the possibility of developing a novel effective vaccine against hantaviruses, we constructed pro ...200818479786
[3 cases of hemorrhagic fever with renal syndrome morbidity].viral origin of hemorrhagic fever with renal syndrome (hfrs) is known since 40th years of last century. also it is known that contamination of person is performed mainly by air pollution way. we observed a focus with 3 hfrs cases (with laboratory confirmation--antibodies to hfrs hantavirus were found) in one family with one lethal outcome. contamination of patients occurred by air pollution way during stocking vegetables contaminated with rodent's excrements. lethal outcome was stipulated by inc ...200818494292
[hantavirus infections].hantaviruses are enveloped single-stranded ribonucleic acid (rna) viruses that belong to the family bunyaviridae. they are widely distributed and maintained in populations of specific insectivores or rodent host species (mice or rats). in their natural host, hantaviruses develop persistent and asymptomatic infections with lifelong virus shedding in excreta. transmission to humans occurs via direct contact with faeces, urine and saliva of infected rodents, in particular by inhaling virus contamin ...200818497244
[myocardial infarction-like electrocardiogram in acute hantavirus infection. suspected secondary peri-myocarditis].a 19-year-old man was admitted to our hospital because of fever and exhaustion. during his hospital stay he had pain in the back and flanks.200818509800
[fever, nosebleeding and myalgic abdominal pain].a 38 year old patient presented with fever, myalgic abdominal pain, nose bleeding and acute renal failure since five days. a combination of thrombocytopenia, proteinuria, elevated crp and creatinin is common in hemorrhagic fever with renal syndrome (hfrs) due to hantavirus infection. the benigne form is called nephropathia epidemica. dialysis is infrequently required by patients with the puumala virus. other infection (e.g. malaria, leptospirosis, yellow fever) and systemic diseases (e.g. collag ...200818548800
development of a serotyping elisa system for thailand virus distinguish thailand virus infection from infections with other hantaviruses, we established an elisa serotyping system using a truncated nucleocapsid protein of thailand virus lacking 49 amino acids at the n-terminus. in evaluations using patient and rodent sera, thailand virus infection was readily distinguished from hantaan and seoul virus infections. therefore, this elisa system is an effective alternative to neutralization tests.200818551243
accumulation of point mutations and reassortment of genomic rna segments are involved in the microevolution of puumala hantavirus in a bank vole (myodes glareolus) population.the genetic diversity of puumala hantavirus (puuv) was studied in a local population of its natural host, the bank vole (myodes glareolus). the trapping area (2.5 x 2.5 km) at konnevesi, central finland, included 14 trapping sites, at least 500 m apart; altogether, 147 voles were captured during may and october 2005. partial sequences of the s, m and l viral genome segments were recovered from 40 animals. seven, 12 and 17 variants were detected for the s, m and l sequences, respectively; these r ...200818559935
clinical course and laboratory parameters of the first dobrava-belgrade hantavirus infection imported to germany. 200818218336
new and old world hantaviruses differentially utilize host cytoskeletal components during their life cycles.recently we reported that the n protein of the old world hantavirus, hantaan (htnv), traffics on microtubules to the er-golgi intermediate compartment (ergic) prior to its movement to the golgi and requires an intact ergic for viral replication. we have extended these studies to the new world hantaviruses, andes virus (andv) and black creek canal virus (bccv), and an additional old world hantavirus, seoul virus (seov). these studies support microtubule-dependent trafficking of the n protein to e ...200818234268
development of recombinant canine adenovirus type-2 expressing the gn glycoprotein of seoul virus.seoul virus glycoprotein gn is a major structural protein and candidate antigen of hantavirus that induces a highly immunogenic response for hantavirus vaccine. in this study, a replication-competent recombinant canine adenovirus type-2 expressing gn was constructed by the in vitro ligation method. the gn expression cassette, including the human cytomegalovirus (hcmv) promoter/enhancer and the sv40 early mrna polyadenylation signal, was cloned into the sspi site of the e3 region which is not ess ...200818249007
Displaying items 401 - 500 of 2786