TitleAbstractYear(sorted descending)
[construction of a recombinant adenovirus carrying the hantaan virus glycoprotein g2 gene and its expression in hela cells].we constructed the recombinant adenovirus expressing the glycoprotein g2 of hantaan virus. firstly we obtained coding gene fragment of g2 by pcr, and subsequently inserted the gene of interest into the adenoviral pshuttle vector pad5-cmv. then we co-transfected the recombinant pshuttle vector and adenovirus skeleton plasmid into hek293 cells by calcium phosphate precipitation method. after the recombinant adenovirus were packaged and amplified in hek293 cells, we observed the expression of repor ...200920112705
simultaneous detection of cdc category "a" dna and rna bioterrorism agents by use of multiplex pcr & rt-pcr enzyme hybridization assays.assays to simultaneously detect multiple potential agents of bioterrorism are limited. two multiplex pcr and rt-pcr enzyme hybridization assays (mpcr-eha, mrt-pcr-eha) were developed to simultaneously detect many of the cdc category "a" bioterrorism agents. the "bio t" dna assay was developed to detect: variola major (vm), bacillus anthracis (ba), yersinia pestis (yp), francisella tularensis (ft) and varicella zoster virus (vzv). the "bio t" rna assay (mrt-pcr-eha) was developed to detect: ebola ...200920224751
the occurrence of hemorrhagic fever with renal syndrome in southern parts of bosnia and herzegovina.bosnia and herzegovina (b&h) has been known as an endemic region for hemorrhagic fever with renal syndrome (hfrs) for over 50 years. multiple epidemics of this disease have been registered so far, especially in endemic parts of central and northeastern bosnia, as well as the sarajevo region. seroepidemiological investigations demonstrate naturalization of hantaviruses and their wide spread in b&h. however, there are no studies from the southern areas of b&h, and endemic foci of this disease are ...200920120399
[expression of partial s gene of hantavirus z10 in pichia pastoris].the purpose of this study is to express partial s gene of hantavirus z10.200920718348
bayou virus detected in non-oryzomyine rodent hosts: an assessment of habitat composition, reservoir community structure, and marsh rice rat social the united states, bayou virus (bayv) ranks second only to sin nombre virus (snv) in terms of hantavirus pulmonary syndrome (hps) incidents, having been confirmed in cases from texas and louisiana since its discovery in 1994. this study on bayv infection among sympatric, non-oryzomyine rodents ("spillover") in freeport, tx, is the first to link patterns of hantavirus interspecific spillover with the spatiotemporal ecology of the primary host (marsh rice rat, oryzomys palustris). mark-recaptur ...200920836801
microhabitat characteristics of akodon montensis, a reservoir for hantavirus, and hantaviral seroprevalence in an atlantic forest site in eastern paraguay.hantaviruses may cause serious disease when transmitted to humans by their rodent hosts. since their emergence in the americas in 1993, there have been extensive efforts to understand the role of environmental factors on the presence of these viruses in their host rodent populations. hps outbreaks have been linked to precipitation, but climatic factors alone have not been sufficient to predict the spatial-temporal dynamics of the environment-reservoir-virus system. using a series of mark-recaptu ...200920836810
molecular epidemiological and serological studies of hantavirus infection in northern vietnam.the distribution of anti-hantavirus antibodies in humans and rodents in northern vietnam was examined. in total, 837 serum samples from healthy humans (617) and patients with fever (220), living in six different areas were screened for igg antibodies against hantaan or seoul virus (seov) by elisa, ifa, and western blot analysis. antibody-positive sera were identified in 7/617 (1.1%) healthy donors, 5/150 port workers in the port of hai phong, and 2/185 residents of ha nam province. in comparison ...200919887743
vaccines for hantaviruses.hantaviruses are assigned to categories a and c on the national institute of allergy and infectious diseases (niaid) priority pathogens list and to category c on the centers for disease control and prevention (cdc) emergency preparedness and response list of boterrorism agents/diseases. these rodent-borne viruses are members of the family bunyaviridae and are transmitted to humans in aerosols of rodent excreta. there are more than 20 recognized hantaviruses, some of which are associated with one ...200919837289
domestically acquired seoul virus causing hemorrhagic fever with renal syndrome-maryland, 2008.hantaviruses are rodent-borne viruses capable of causing human disease. the seoul virus is a hantavirus that causes hemorrhagic fever with renal syndrome in east asia. to our knowledge, we report the first domestically acquired case of hemorrhagic fever with renal syndrome caused by the seoul virus, confirmed by serology testing, reverse-transcriptase polymerase chain reaction, and nucleotide sequence analysis. the patient presented with myalgias and fever, and developed acute renal failure.200919848600
co-circulation of three pathogenic hantaviruses: puumala, dobrava, and saaremaa in hungary.hantaviruses (bunyaviridae) cause hemorrhagic fever with renal syndrome (hfrs) in eurasia and hantavirus (cardio)pulmonary syndrome (hcps) in the americas. hfrs is caused by hantaan virus (htnv), seoul virus (seov), dobrava virus (dobv), saaremaa virus (saav), and puumala virus (puuv). of those, only htnv is not present in europe. in recent years, hantaviruses, described in other parts of europe, were also detected at various locations in hungary. to study the genetic properties of hungarian han ...200919856478
[infectious diseases and climate change].climate changes will likely have an impact on the spectrum of infectious diseases in europe. we may see an increase in vector-borne diseases, diseases spread by rodents such as hantavirus, and food- and water-borne diseases. as the effects of climate changes are likely to occur gradually, a modern industrialised country such as denmark will have the opportunity to adapt to the expected changes.200919857396
molecular diagnostics of hemorrhagic fever with renal syndrome during a dobrava virus infection outbreak in the european part of russia.a large outbreak of hemorrhagic fever with renal syndrome (hfrs) occurred in the winter of 2006-2007 in a region southeast of moscow in central european russia. of the 422 patients with hfrs investigated in this study, 58 patients were found to be infected by puumala virus, whereas as many as 364 were infected by dobrava-belgrade virus (dobv). early serum samples from 10 dobv-infected patients were used for nucleic acid amplification, which was successful for 5 patients. molecular analyses demon ...200919828747
using satellite images of environmental changes to predict infectious disease outbreaks.recent events clearly illustrate a continued vulnerability of large populations to infectious diseases, which is related to our changing human-constructed and natural environments. a single person with multidrug-resistant tuberculosis in 2007 provided a wake-up call to the united states and global public health infrastructure, as the health professionals and the public realized that today's ease of airline travel can potentially expose hundreds of persons to an untreatable disease associated wit ...200919788799
hantavirus infection in the republic of georgia.we describe a laboratory-confirmed case of hantavirus infection in the republic of georgia. limited information is available about hantavirus infections in the caucasus, although the infection has been reported throughout europe and russia. increasing awareness and active disease surveillance contribute to our improved understanding of the geographic range of this pathogen.200919788822
hantavirus pulmonary syndrome in brasilia periphery: a diagnostic challenge.hantavirus pulmonary syndrome (hps) is an emerging rodent-borne disease in the american continent, characterized by acute respiratory distress and a high case-fatality ratio. the present work describes a case of hps, with favorable outcome, whose initial features were mistaken for leptospirosis or other less severe acute infections.200919801809
evaluation of an enzyme-linked immunosorbent assay based on araraquara virus recombinant nucleocapsid protein.laboratory diagnosis of hantavirus cardiopulmonary syndrome (hcps) in brazil has been performed mostly by detection of igm antibodies to recombinant antigen purified from sin nombre virus and andes virus (andv). recently, a recombinant nucleocapsid (rn) protein of araraquara virus (arav), a brazilian hantavirus, was obtained in escherichia coli. to evaluate arav rn as antigen for antibody detection, serum samples from 30 patients from argentina seropositive for hantavirus were tested. all sample ...200919635882
[survey on host animal and molecular epidemiology of hantavirus in chuxiong prefecture, yunnan province].to determine the hosts of hantavirus (hv) and its molecular epidemiological characteristics, to provide evidence for prevention and control on hemorrhagic fever with renal syndrome (hfrs).200919642377
production and characterization of monoclonal antibodies against the recombinant nucleoprotein of araucaria hantavirus.hantaviruses are rodent-borne rna viruses that cause hemorrhagic fever with renal syndrome (hfrs) or hantavirus pulmonary syndrome (hps). from the first detection of infection in brazil in 1993 until 2009, 1161 cases of hps have been reported, with mortality rates of around approximately 40%. currently, due to the absence of a vaccine or specific antiviral therapy, the only way to reduce mortality by hantavirus infection is a fast and precise diagnosis that allows for supportive clinical health ...200919654026
genetic characterization of a juquitiba-like viral lineage in oligoryzomys nigripes in rio de janeiro, brazil.hantaviruses, family bunyaviridae, are rodent-borne rna viruses that have caused cases of hantavirus cardiopulmonary syndrome (hcps) in various regions of the americas. there are five hantaviral lineages associated with hcps in brazil: juquitiba virus (juqv), araraquara virus (arav), laguna negra-like virus (lnv), castelo dos sonhos virus (casv), and anajatuba virus (anajv). three additional hantaviruses have been described in rodents alone: rio mearim virus, jaborá virus, and a hantavirus linea ...200919660427
degradation and aggresome formation of the gn tail of the apathogenic tula hantavirus.the cytoplasmic tails of envelope glycoprotein gn of pathogenic hantaviruses but not of the apathogenic prospect hill virus (phv) were recently reported to be proteasomally degraded in simian cos7 cells. here, we show that the cytoplasmic tails of the glycoproteins of the apathogenic hantaviruses tula virus (tulv) and phv are also degraded through the ubiquitin-proteasome pathway, both in human hek-293 and in simian vero e6 cells. tulv gn tails formed aggresomes in cells with proteasomal inhibit ...200919675185
[pulmonary and cardiovascular syndrome due to hantavirus: clinical aspects of an emerging disease in southeastern brazil].pulmonary and cardiovascular syndrome due to hantavirus is a disease caused by inhalation of aerosols from the excreta of wild rodents contaminated by viruses of the bunyaviridae family. we studied the clinical and laboratory manifestations of 70 cases that occurred in the region of ribeirão preto, sp, brazil, between 1998 and 2007. the frequency of symptoms was as follows: dyspnea (87%), fever (81%), coughing (44%), headache (34%), tachycardia (81%), low arterial blood pressure (56%), metabolic ...200919684976
healthcare-associated atypical pneumonia.atypical pneumonia was first described in 1938, and over time, mycoplasma, legionella, and chlamydophila were the agents commonly linked with community-associated atypical pneumonia. however, as technology has improved, so has our understanding of this clinical entity. it is now known that there are many agents linked with atypical pneumonia in the community, and many of these agents are also major causes of healthcare-associated pneumonia. this article discusses the history, epidemiology, and p ...200919199189
survey for antibody to hantaviruses in tamaulipas, mexico.wild rodents (n=248) were trapped in two ecologically distinct sites at el cielo biosphere reserve in the state of tamaulipas, mexico, during the summer of 2003. samples from 199 individuals were tested for hantavirus antibodies by an indirect enzyme-linked immunosorbent assay (elisa). hantavirus antibodies to recombinant sin nombre virus nucleocapsid protein were found in seven rodents (3.5%) of a single species, peromyscus levipes. antibody-positive rodents were found only in the cloud forest ...200919204351
young man with kidney failure and hemorrhagic interstitial nephritis. 200919556044
hantavirus infection and habitat associations among rodent populations in agroecosystems of panama: implications for human disease risk.hantavirus cardiopulmonary syndrome (hcps), which is caused by infection with choclo virus, is uncommon in panama, yet seropositivity among rural residents is as high as 60%. to clarify the environmental risk factors favoring rodent-to-human transmission, we tested serum from 3,067 rodents captured over a five-year period for antibodies against recombinant n protein of hantavirus by enzyme immunoassay and strip immunoblot. among 220 seropositive rodents, oligoryzomys fulvescens, the reservoir of ...200919556568
hantavirus species in india: a retrospective study.hantaviruses cause hemorrhagic fever with renal syndrome in europe and asia. there are about 20 documented hantavirus species and newer species are being described worldwide, especially in non-rodent reservoirs, i.e shrews. focus reduction neutralization test is the classical serotyping technique for hantavirus. however, this study employs a previously established serotyping elisa, to retrospectively analyze known hantavirus igg reactive samples for infecting serotypes. the result suggests prese ...200919736405
[serological characteristics of hantaviruses from clinical specimens analyzed in 1998-2008 in the department of virology, public health institute, ostrava].in 1998 - 2008, serum samples from 464 patients, predominantly residents of the moravia-silesian region, were examined in the department of virology, public health institute in ostrava, using enzyme-linked immunosorbent assay (elisa) for the detection of specific anti-hantavirus antibodies. seropositivity was ascertained in 41 persons (8.8%). more patients tested seropositive against hantaan virus (24, i.e. 58.5%) than against puumala virus (13, i.e. 31.7%). four persons were reactive against bo ...200919750821
inhibition of tnf-alpha-induced activation of nf-kappab by hantavirus nucleocapsid proteins.hantaviruses cause two human diseases thought to be immune-mediated: hemorrhagic fever with renal syndrome (hfrs) and hantavirus pulmonary syndrome (hps). we recently reported that the nucleocapsid (n) protein of hfrs-causing hantaan virus (htnv) inhibits tumor necrosis factor-alpha (tnf-alpha) activation of nuclear factor-kappab (nf-kappab). here we measured the ability of other hantaviral n proteins to similarly interfere with the inflammatory process of tnf-alpha. we found that like htnv n, t ...200919751407
identification of oligopeptides mimicking the receptor-binding domain of hantaan virus envelope glycoprotein from a phage-displayed peptide library.attachment of enveloped viruses to cells is triggered by the receptor-binding domain (rbd) on envelope glycoproteins (gp) binding to receptors located on the cell surface. to date, recognized receptors and rbd of hantaan virus (htnv) have not been exactly defined. in this study, one monoclonal antibody (mab) 3g1 possessing high neutralizing activity, which is directed against htnv envelope glycoprotein g2, was used to determine the crucial motif of rbd. peptide ligands binding to mab 3g1 were se ...200919767836
complete nucleotide sequence of four rna segments of fig mosaic virus.the complete sequence of four viral rna segments of fig mosaic virus (fmv) was determined. each of the four rnas comprises a single open reading frame (orf) 7,093, 2,252, 1,490 and 1,472 nucleotides in size, respectively. these orfs encode the following proteins in the order: rna-dependent rna polymerase (p1 264 kda), a putative glycoprotein (p2 73 kda), a putative nucleocapsid protein (p3 35 kda) and a protein with unknown function (p4 40.5 kda). all rna segments possess untranslated regions co ...200919777155
[cloning and expression of the nucleoprotein gene of puumala-like virus].in order to detect hokkaido virus (hokv), a recombinant baculovirus containing the nucleoprotein (np) gene of hokv was constructed, and then the np was expressed in insect cell.200919565881
[study on the difference of genes and the type identification of hantavirus from lishui, zhejiang province].to isolate hantavirus from lishui county--one of the epidemic regions for hemorrhagic fever with renal syndrome (hfrs), in zhejiang province, and to identify the serotype and molecular/biological characteristics of a new htn subtype hantavirus (hv) strains, hopefully to provide evidence for hfrs prevention and therapy.200919565882
hantaviral infections of rodents: possible scenarios. 200919568690
the first established focus of hantavirus infection in poland, 2007.the first hantavirus infection outbreak in poland (with different seroetiology) was identified between august-december 2007. thirteen cases were reported in southeast poland: 12 cases in the carpathians bordering with northeast slovakia, mainly in the forested areas of the bieszczady mountains, and one case approximately 100-120 kilometres north from the others, in the adjacent sub-carpathian region. four additional cases of past infection were identified retrospectively, based on the presence o ...200919572481
evolutionary insights from a genetically divergent hantavirus harbored by the european common mole (talpa europaea).the discovery of genetically distinct hantaviruses in shrews (order soricomorpha, family soricidae) from widely separated geographic regions challenges the hypothesis that rodents (order rodentia, family muridae and cricetidae) are the primordial reservoir hosts of hantaviruses and also predicts that other soricomorphs harbor hantaviruses. recently, novel hantavirus genomes have been detected in moles of the family talpidae, including the japanese shrew mole (urotrichus talpoides) and american s ...200919582155
a single immunization with a recombinant canine adenovirus type 2 expressing the seoul virus gn glycoprotein confers protective immunity against seoul virus in mice.seoul virus (seov), a member of hantavirus genus, is one of the causative agents of hemorrhagic fever with renal syndrome (hfrs) and afflicts tens of thousands of people annually. in this paper, we evaluate the immune response induced by a replication-competent recombinant canine adenovirus type 2 expressing the gn protein of seov (rcav-2-gn) in balb/c mice. sera from immunized mice contained neutralizing antibodies that could specifically recognize seov and neutralize its infectivity in vitro. ...200919583957
hantavirus infection: a case report from india.the clinical presentation of hantavirus infections in india is unclear. we report here a case of hantavirus infection in a 46 year old quarry worker presenting with fever, abdominal pain, jaundice, thrombocytopenia and renal dysfunction. seroconversion and rising anti-hantavirus igg titers were taken as evidence of hantavirus infection. clinicians should consider hantavirus infections in the differential diagnosis of acute febrile illness along with scrub typhus, leptospirosis and dengue.200919584514
analysis of the immune response to hantaan virus nucleocapsid protein c-terminal-specific cd8(+) t cells in patients with hemorrhagic fever with renal syndrome.hantaan virus (htnv), the prototype member of the hantavirus genus in the family bunyaviridae, causes hemorrhagic fever with renal syndrome (hfrs), which is characterized by capillary leakage, hemorrhage, and renal injury, and is an important public health problem in china. some kinds of immune cells, particularly cd8(+) t cells, are involved in the pathogenesis of hantavirus infection. the nucleocapsid protein (np) of the hantavirus is the most conserved structural protein and the most abundant ...200919594396
temporal and geographic evidence for evolution of sin nombre virus using molecular analyses of viral rna from colorado, new mexico and montana.all viruses in the family bunyaviridae possess a tripartite genome, consisting of a small, a medium, and a large rna segment. bunyaviruses therefore possess considerable evolutionary potential, attributable to both intramolecular changes and to genome segment reassortment. hantaviruses (family bunyaviridae, genus hantavirus) are known to cause human hemorrhagic fever with renal syndrome or hantavirus pulmonary syndrome. the primary reservoir host of sin nombre virus is the deer mouse (peromyscus ...200919602267
hantaviruses and climate change.most hantaviruses are rodent-borne emerging viruses. they cause two significant human diseases, haemorrhagic fever with renal syndrome in asia and europe, and hantavirus cardiopulmonary syndrome in the americas. very recently, several novel hantaviruses with unknown pathogenic potential have been identified in africa and in a variety of insectivores (shrews and a mole). because there is very limited information available on the possible impact of climate change on all of these highly dangerous p ...200919604276
hantavirus reservoir oligoryzomys longicaudatus spatial distribution sensitivity to climate change scenarios in argentine patagonia.oligoryzomys longicaudatus (colilargo) is the rodent responsible for hantavirus pulmonary syndrome (hps) in argentine patagonia. in past decades (1967-1998), trends of precipitation reduction and surface air temperature increase have been observed in western patagonia. we explore how the potential distribution of the hantavirus reservoir would change under different climate change scenarios based on the observed trends.200919607707
a habitat-based model for the spread of hantavirus between reservoir and spillover habitat-based models for spread of hantavirus are developed which account for interspecies interaction. existing habitat-based models do not consider interspecies pathogen transmission, a primary route for emergence of new infectious diseases and reservoirs in wildlife and man. the modeling of interspecies transmission has the potential to provide more accurate predictions of disease persistence and emergence dynamics. the new models are motivated by our recent work on hantavirus in rodent c ...200919616014
increased host species diversity and decreased prevalence of sin nombre virus.emerging outbreaks of zoonotic diseases are affecting humans at an alarming rate. until the ecological factors associated with zoonoses are better understood, disease emergence will continue. for lyme disease, disease suppression has been demonstrated by a dilution effect, whereby increasing species diversity decreases disease prevalence in host populations. to test the dilution effect in another disease, we examined 17 ecological variables associated with prevalence of the directly transmitted ...200919624913
risk factors for human infection with puumala virus, southwestern germany.puumala virus, which causes nephropathia epidemica (ne), is the most prevalent hantavirus in germany; bank voles serve as the main reservoir. during 2001-2007, most ne cases reported from germany occurred in the southwestern state of baden-württemberg. we investigated the influence of bank vole habitats (beech forest, seed plants), vole food supply (beechnut mast), climate factors (winter and spring temperatures), and human population density on spatial and temporal occurrence of ne cases in bad ...200919624917
sensitivity of andes hantavirus to antiviral effect of human saliva. 200919624946
the hantavirus glycoprotein g1 tail contains dual cchc-type classical zinc fingers.hantaviruses are distributed worldwide and can cause a hemorrhagic fever or a cardiopulmonary syndrome in humans. mature virions consist of rna genome, nucleocapsid protein, rna polymerase, and two transmembrane glycoproteins, g1 and g2. the ectodomain of g1 is surface-exposed; however, it has a 142-residue c-terminal cytoplasmic tail that plays important roles in viral assembly and host-pathogen interaction. here we show by nmr, circular dichroism spectroscopy, and mutagenesis that a highly con ...200919179334
potential renal sequelae in survivors of hantavirus cardiopulmonary syndrome.although other hantaviruses are associated with renal manifestations, hantavirus cardiopulmonary syndrome (hcps) has not been associated with such sequelae. the hcps survivors were prospectively evaluated for renal complications. subjects underwent yearly evaluation, laboratory studies, and 24-hour urine collection. thirty subjects were evaluated after recovery from hcps with the first follow-up at a median of 7.4 months after discharge. subjects were a wide age range (18-51) but had an equal ge ...200919190227
seoul virus and hantavirus disease, shenyang, people's republic of outbreak of hemorrhagic fever with renal syndrome (hfrs) occurred among students in shenyang pharmaceutical university in 2006. we conducted a study to characterize etiologic agents of the outbreaks and clarify the origin of hantaviruses causing infections in humans and laboratory animals. immunoglobulin (ig) m or igg antibodies against seoul virus (seov) were detected in the serum samples of all 8 patients. igg antibodies against hantavirus were also identified in laboratory rats, which were ...200919193263
hantaan virus triggers tlr3-dependent innate immune responses.immediately after viral infection, innate responses including expression of ifn-alpha/beta and ifn-stimulated genes (isgs) are elicited ubiquitously by recruitment of specific pathogen recognition receptors. the velocity to induce ifn-alpha/beta and isgs in response to an infection is often decisive for virulence. interestingly, in primary endothelial cells isgs are induced later by hantaviruses pathogenic to humans than those considered to be nonpathogenic or of low virulence. here we demonstra ...200919234180
acute febrile illness caused by hantavirus: serological and molecular evidence from of hantavirus infections in india is in its early stages. as early symptoms of hantavirus disease can be non-specific and the diagnosis confirmed only by laboratory testing, use of appropriate diagnostic tools is important. to improve the diagnosis of hantavirus infections in india, commercial elisa systems followed by indirect immunofluorescence assays were used to detect anti-hantavirus igm and igg in samples from patients with acute febrile illness. of 347 patients tested, 5.2% showed s ...200919237179
hantavirus infections in europe: from virus carriers to a major public-health europe, hantavirus disease or hemorrhagic fever with renal syndrome is an endemic zoonosis that affects tens of thousands of individuals each year. the causative agents are viruses of the genus hantavirus, family bunyaviridae, rodents and insectivores act as carriers. in all european countries there is a seroprevalence for hantaviruses in the general population but not all countries report cases. here, we give an overview of the hantavirus situation in europe.200919254169
[rodents and rodent associated disease vectors: the network of "rodent carrying pathogens" introduces itself]. 200919263029
an outbreak caused by hantavirus in the black sea region of turkey, january-may 2009.we present a preliminary report of 12 laboratory-confirmed cases of haemorrhagic fever with renal syndrome (hfrs) in turkey, diagnosed between january and may 2009 according to the clinical symptoms and serological confirmation. studies are still ongoing to better understand the dynamics of the reservoir population as well as the epidemiological characteristics and risk factors among humans.200919460288
gold nanoparticle enhanced immuno-pcr for ultrasensitive detection of hantaan virus nucleocapsid protein.a functionalized gold nanoparticle (gnp) enhanced ultrasensitive immuno-pcr assay (gnp-ipcr on elisa plate), which was modified from the recent developed bio-barcode assay (bca) technique, was developed to detect hantaan virus nucleocapsid protein (hnp). during the assay, the target antigen hnp was captured by a polyclonal antibody coated on elisa microplate wells, followed by adding gnp dually modified with oligonucleotides and a hnp specific monoclonal antibody l13 (mab l13) to form a sandwich ...200919467237
serological microarray for detection of hsv-1, hsv-2, vzv, and cmv antibodies.the seroprevalence of human herpesviruses is high and reactivations occur frequently. a microarray was designed and tested for the detection of igg and igm antibodies for puumala hantavirus (puuv) and igg antibodies against four herpesviruses. initially, a microarray platform was set up using an unrelated in-house antigen, puuv recombinant nucleocapsid protein, to optimize the protocol for the detection of antibodies. detection of the four herpesviruses was set up in a microarray using the recom ...200919477202
genetic diversity and geographic distribution of hantaviruses in russia.haemorrhagic fever with renal syndrome (hfrs) is the most prevalent zoonotic disease in russia. it is caused by several hantavirus species hosted by small rodents. we describe spatial and temporal patterns of hfrs incidence in the russian federation, and the geographic distribution of prevalent hantavirus species: puumala (puuv) and dobrava (dobv). partial sequencing of nucleocapsid and glycoprotein genes of 117 puuv strains and 78 dobv strains revealed several distinct genetic subgroups. the rn ...200919486318
testing mechanisms of the dilution effect: deer mice encounter rates, sin nombre virus prevalence and species diversity.species diversity has been shown to decrease prevalence of disease in a variety of host-pathogen systems, in a phenomenon termed the dilution effect. several mechanisms have been proposed by which diversity may decrease prevalence, though few have been tested in natural host-pathogen systems. we investigated the mechanisms by which diversity influenced the prevalence of sin nombre virus (snv), a directly transmitted virus in deer mice (peromyscus maniculatus). we monitored both intra and intersp ...200919495881
[the first french hemorrhagic fever with renal syndrome in pregnant woman].the seroprevalence of hantavirus in france is very low. the hantavirus is the causative agent of hemorrhagic fever with renal syndrome (hfrs). in 2005, we report the first french hfrs case in a pregnant woman presenting with influenza like symptoms in an endemic area associated with suggestive biologic abnormalities. she was delivered by emergency cesarean section. her child had a neonatal respiratory distress, thrombocytopenia and elevated liver enzymes levels, which suggest a transplacental ha ...200919497685
phage display selection of cyclic peptides that inhibit andes virus infection.specific therapy is not available for hantavirus cardiopulmonary syndrome caused by andes virus (andv). peptides capable of blocking andv infection in vitro were identified using antibodies against andv surface glycoproteins gn and gc to competitively elute a cyclic nonapeptide-bearing phage display library from purified andv particles. phage was examined for andv infection inhibition in vitro, and nonapeptides were synthesized based on the most-potent phage sequences. three peptides showed leve ...200919515773
theory of possible effects of the allee phenomenon on the population of an epidemic reservoir.we investigate possible effects of high-order nonlinearities on the shapes of infection refugia of the reservoir of an infectious disease. we replace fisher-type equations that have been recently used to describe, among others, the hantavirus spread in mouse populations by generalizations capable of describing allee effects that are a consequence of the high-order nonlinearities. after analyzing the equations to calculate steady-state solutions, we study the stability of those solutions and comp ...200919518251
knowledge and practices about hantavirus pulmonary syndrome in a cluster of japanese communities in provide information on hantavirus pulmonary syndrome (hps) knowledge and practices among a cluster of the rural japanese communities near la plata in central argentina, an area with confirmed hps cases. particular emphasis was placed on testing the hypothesis that there would be differences between household use of hps prevention measures based on the head-of-household's knowledge of hps.200919531307
[climate changes and emerging diseases. what new infectious diseases and health problem can be expected?].increasing temperatures, but also other climatic factors, will have an impact on human health. apart from the direct consequences of extreme weather conditions (e.g., heat-related fatalities), indirect health consequences in the long-term are also of great importance. in addition to a likely increase in allergic diseases and additional complications in the course of cardiovascular and respiratory diseases, infectious diseases are of particular interest. in germany, endemic pathogens, such as han ...200919536444
genotyping of hantaviruses occurring in linyi, china, by nested rt-pcr combined with single-strand conformation polymorphism analysis.the hemorrhagic fever with renal syndrome (hfrs) incidence rate still holds in high level in some areas of the world, but a reliable and simple typing method that can be used in clinical diagnosis and epidemiologic surveys is not available. in this study, 48 serum samples were collected from patients with hfrs in linyi area, china, that is seriously affected by this disease. the collected samples were analyzed by nested rt-pcr combined with single-strand conformation polymorphism (sscp) for geno ...200919537913
crossing borders in health communication research: toward an ecological understanding of context, complexity, and consequences in community-based health education in the u.s.-mexico the past 15 years, researchers in health information dissemination have made significant strides in addressing context, complexity, and consequences (sharf's "3 cs") in theory, research, and praxis. the goal of integrating the 3 cs in this field, however, remains a challenge. in this article, the authors present 6 principles of a postmodern ecological approach to health information dissemination that attempts such integration. the authors outline proposed principles and use a multiyear, inter ...200920183369
[genotyping and sequence analysis on g2 segment of hantavirus carried by rat in hebei province].to know the genotype and subtype of hantavirus (hv) carried by host animals in hfrs natural epidemic area of hebei province.200920104766
hsp70 induced by hantavirus infection interacts with viral nucleocapsid protein and its overexpression suppresses virus infection in vero e6 cells.hantavirus (htv) infection is known to induce innate cellular response, a more specified cellular response in the host cells. however, whether it stimulates synthesis of stress proteins, particularly associations of viral proteins, is entirely unknown. the primary focus of this research is using vero e6 cells infected with hantaan 76-118 (htnv) as an in vitro infection model to examine the individual contribution of htv infection to heat shock response. this study shows that htnv infection rapid ...200919956449
perspectives on scrub typhus, tick-borne pathogens, and hantavirus in the republic of korea. 200920088033
hantavirus pulmonary syndrome in five pediatric patients - four states, 2009.hantavirus pulmonary syndrome (hps) is a reportable infectious disease with a high case-fatality rate, transmitted to humans by exposure to rodents. each year, 20--40 cases of hps occur in the united states; cases in persons aged <17 years make up fewer than 7% of those cases, and cases in children aged <10 years are exceptionally rare. cdc received reports of five pediatric cases of hps occurring during may 16--november 25, 2009, among children aged 6--14 years from arizona, california, colorad ...200920032925
epidemiological study of hantavirus infection in the samara region of european russia.european russia is a highly endemic area of hemorrhagic fever with renal syndrome (hfrs), a rodent-borne zoonotic disease, caused by hantaviruses. in total, 145 small mammals of four species (myodes glareolus, apodemus flavicollis, a. agrarius, and a. uralensis) were trapped in the samara region of european russia in august 2005 and examined for the presence of hantavirus (hv). anti-hv antibodies were found in six of 68 (8.8%) m. glareolus and in one of 19 (5.3%) a. flavicollis by indirect immun ...200920046023
milder winters in northern scandinavia may contribute to larger outbreaks of haemorrhagic fever virus.the spread of zoonotic infectious diseases may increase due to climate factors such as temperature, humidity and precipitation. this is also true for hantaviruses, which are globally spread haemorrhagic fever viruses carried by rodents. hantaviruses are frequently transmitted to humans all over the world and regarded as emerging viral diseases. climate variations affect the rodent reservoir populations and rodent population peaks coincide with increased number of human cases of hantavirus infect ...200920052429
[geographic expansion of hantavirus pulmonary syndrome in argentina. the southernest case report].since 1995 more than 1000 cases of hantavirus pulmonary syndrome (hps) were reported in argentina, a severe disease and often fatal to humans. most cases were associated with andes virus (and) that caused few events of person-to-person transmission. several lineages of pathogenic and viruses have been described, including and south, hosted by the rodent oligoryzomys longicaudatus which affects the patagonian region of argentina and chile. we studied the clinical and epidemiological characteristi ...200920053606
genetic diversity and phylogeography of seewis virus in the eurasian common shrew in finland and hungary.recent identification of a newfound hantavirus, designated seewis virus (swsv), in the eurasian common shrew (sorex araneus), captured in switzerland, corroborates decades-old reports of hantaviral antigens in this shrew species from russia. to ascertain the spatial or geographic variation of swsv, archival liver tissues from 88 eurasian common shrews, trapped in finland in 1982 and in hungary during 1997, 1999 and 2000, were analyzed for hantavirus rnas by reverse transcription-polymerase chain ...200919930716
seasonal dispersal patterns of sylvan deer mice (peromyscus maniculatus) within montana rangelands.we examined seasonal dispersal patterns and timing of new infections of sin nombre virus (snv), as determined by recent acquisition of antibodies (seroconversion), in deer mice (peromyscus maniculatus) at two montana rangeland study sites over three years, 2004-2007. one study site was located in grassland habitat, and the other was located in shrub-steppe. in montana, both of these habitats are commonly associated with peridomestic environments (in and around buildings). peridomestic environmen ...200919901376
[research on hantanvirus infection of rodents in junan county, shandong province]. 200919957614
sympatry of 2 hantavirus strains, paraguay, explore geographic and host-taxonomic patterns of hantaviruses in paraguay, we established sampling sites in the mbaracayu biosphere reserve. we detected jabora virus and itapua37/juquitiba-related virus in locations approximately 20 m apart in different years, which suggested sympatry of 2 distinct hantaviruses.200919961679
cross-sectional survey of hantavirus infection, brazil.a cross-sectional serosurvey was conducted to assess the proportion of persons exposed to hantaviruses in a virus-endemic area of the state of minas gerais, brazil. findings of this study suggested the presence of > or =1 hantaviruses circulating in this region causing hantavirus pulmonary syndrome, mild disease, or asymptomatic infection.200919961680
dobrava-belgrade virus spillover infections, germany.we present the molecular identification of apodemus agrarius (striped field mouse) as reservoir host of the dobrava-belgrade virus (dobv) lineage dobv-aa in 3 federal states of germany. phylogenetic analyses provided evidence for multiple spillover of dobv-aa to a. flavicollis, a crucial prerequisite for host switch and genetic reassortment.200919961690
hantavirus regulation of endothelial cell functions.hantaviruses cause two vascular permeability-based diseases and primarily infect endothelial cells which form the primary fluid barrier of the vasculature. since hantavirus infections are not lytic, the mechanisms by which hantaviruses cause haemorrhagic fever with renal syndrome (hfrs) or hantavirus pulmonary syndrome (hps) are indeterminate. hps is associated with acute pulmonary oedema and hfrs with moderate haemorrhage and renal sequelae, perhaps reflecting the location of vast microvascular ...200919967132
[thrombocytopenia and acute kidney injury].a 31-year old man is addressed to the emergency department for fever, abdominal pain and vomiting. laboratory tests reveal an inflammatory syndrome, thrombocytopenia, acute kidney injury associated with major proteinuria (more than 3 g/24 h). evaluation will conclude to an acute interstitial nephritis and the serology is positive for hantavirus. this clinical case illustrates differential diagnosis of acute renal failure and reminds the theory of hemorrhagic fever with renal syndrome : usual cli ...200919998799
[from mice to men: the threat of hantavirus infections].hantaviruses are naturally hosted by small mammals. virus transmission to humans can result in the development of hemorrhagic fever with renal syndrome (asia, europe) or hantavirus cardiopulmonary syndrome (the americas). certain hantaviruses can cause life-treatening infections with case fatality indices up to about 50%. different virus types have evolved which use different species of small mammals (mainly but not exclusively rodent species) as natural reservoirs. it is still unknown why these ...200919999378
an experimental test of factors attracting deer mice into buildings.deer mice (peromyscus maniculatus) are the principal reservoir host of sin nombre virus (snv). deer mice use a wide variety of habitats including peridomestic settings in and around human dwellings, their presence in and around homes has been implicated as a risk factor for acquiring hantavirus pulmonary syndrome. deer mice are believed to enter buildings in order to gain access to a variety of resources including food, bedding material, and better thermal microclimates. however, no one has expe ...200920628475
hantavirus disease (nephropathia epidemica) in belgium: effects of tree seed production and climate.recently, human cases of nephropathia epidemica (ne) due to puumala virus infection in europe have increased. following the hypothesis that high reservoir host abundance induces higher transmission rates to humans, explanations for this altered epidemiology must be sought in factors that cause bank vole (myodes glareolus) abundance peaks. in western europe, these abundance peaks are often related to high tree seed production, which is supposedly triggered by specific weather conditions. we evalu ...200918606026
role of the nss protein in the zoonotic capacity of orthobunyaviruses.the family bunyaviridae contains over 350 named isolates, classified into five genera: orthobunyavirus, hantavirus, nairovirus, phlebovirus and tospovirus. the orthobunyavirus genus contains some 170 isolates that are mainly transmitted by mosquitoes and are responsible for a range of disease syndromes in humans including self-limiting febrile illness, encephalitis and haemorrhagic fever. the viruses have a tripartite, negative-sense rna genome. analyses of viruses in four serogroups (bunyamwera ...200918771514
construction and expression of a eukaryotic expression vector containing a fusion gene of the hantaan virus s gene and hsp70 gene.hantavirus (hv) infection leads to a kind of severe systematic syndrome, hemorrhagic fever with renal syndrome (hfrs). heat shock proteins (hsps) can be used as adjuvants assisting soluble antigens to produce specific targets which can be attacked by cytotoxic t lymphocytes. for further research on hfrs vaccine, this study aimed to express hantaan virus nucleocapsid protein (htnv np)-hsp70 fusion protein in cos-7 cells. first, an htnv s gene encoding np was amplified by pcr with a mutated termin ...200918810536
bovine and human-derived passive immunization could help slow a future avian influenza epidemic of human transmitted avian influenza could have casualties on a scale seen in the great spanish influenza pandemic of 1918. this paper proposes that should such occur before effective vaccines and antiviral drugs are available, the outbreak could be significantly slowed by consumption of raw milk produced by herds of pathogen-free lactating cows intranasally inoculated with heat-sterilized sputa pooled from avian influenza patients, supplemented by parenteral serum immune globulin fr ...200918824305
serological and genetic evidence for the presence of seoul hantavirus in rattus norvegicus in flanders, belgium.seoul hantavirus (seov), carried by rattus rattus (black rat) and r. norvegicus (norway, brown rat), was reported to circulate as well as cause hfrs cases in asia. as rattus sp. are present worldwide, seov has the potential to cause human disease worldwide. in europe however, only seov prevalence in rats from france was reported and no confirmed cases of seov infection were published. we here report genetic and serological evidence for the presence of seov virus in brown rat populations in belgi ...200918821445
hantavirus evolution in relation to its rodent and insectivore hosts: no evidence for codivergence.hantaviruses are considered one of the best examples of a long-term association between rna viruses and their hosts. based on the appearance of strong host specificity, it has been suggested that hantaviruses cospeciated with the rodents and insectivores they infect since these mammals last shared a common ancestor, approximately 100 million years ago. we tested this hypothesis of host-virus codivergence in two ways: 1) we used cophylogenetic reconciliation analysis to assess the fit of the viru ...200918922760
more than half of the patients with acute puumala hantavirus infection have abnormal cardiac findings.this study was conducted to determine the frequency, severity and outcome of cardiac findings in patients with acute puumala hantavirus-induced nephropathia epidemica (ne). 70 consecutive, hospital-treated patients with serologically confirmed ne were prospectively examined using serial electrocardiograms (ecg), plasma troponin i, tumour necrosis factor alpha (tnf-alpha), interleukin-6 (il-6), and echocardiography (echo). examinations were repeated after 3 and 12 months. ecg changes were observe ...200918932105
hantaan virus nucleocapsid protein binds to importin alpha proteins and inhibits tumor necrosis factor alpha-induced activation of nuclear factor kappa b.hantaviruses such as hantaan virus (htnv) and andes virus cause two human diseases, hemorrhagic fever with renal syndrome and hantavirus pulmonary syndrome, respectively. for both, disease pathogenesis is thought to be immunologically mediated and there have been numerous reports of patients with elevated levels of proinflammatory and inflammatory cytokines, including tumor necrosis factor alpha (tnf-alpha), in their sera. multiple viruses have developed evasion strategies to circumvent the host ...200919019947
a newly recognized hantavirus in the asian house rat (rattus tanezumi) in indonesia.hantaviral sequences were recovered from the lung tissue of an asian house rat (rattus tanezumi) captured in serang, indonesia. phylogenetic analysis of partial l, m and s segment sequences showed that they belonged to a novel hantavirus provisionally named serang virus (serv). notably, serv is distinct from the hantaviruses associated with rodents of the species rattus: seoul virus associated with rattus norvegicus worldwide and gou virus isolated from rattus rattus in china. instead serv appea ...200919088290
secretory expression and purification of recombinant escherichia coli heat-labile enterotoxin b subunit and its applications on intranasal vaccination of order to further study the b subunit of the escherichia coli heat-labile enterotoxin (ltb), we obtained the ltb gene from pathogenic e. coli, cloned it into the pet22b (+) prokaryotic expression vector, and expressed it as a fusion protein with his tag in e. coli bl21 (de3). the recombinant ltb was expressed and purified by ni2+ affinity chromatography. the biological activity of the purified recombinant ltb was assayed in a series of monosialotetrahexosylganglioside (gm1)-elisa experiments. ...200919089660
central nervous system complications following hanta virus cardiopulmonary syndrome. 200919089788
recent approaches in hantavirus vaccine development.rodent-borne hantaviruses are associated with two main clinical disorders in humans: hemorrhagic fever with renal syndrome and hantavirus cardiopulmonary syndrome. although hantavirus diseases can be life threatening and numerous research efforts are focused on the development of hantavirus prevention, no specific antiviral therapy is yet available and, at this time, no who-approved vaccine has gained widespread acceptance. this review will summarize the current knowledge and recent progress as ...200919093774
characterization of hantaviruses circulating in central 2004, an outbreak of hcps in brazil made hantaviruses a national threat to the rural and urban population. during this outbreak, 164 cases were reported, and 18.3% of them occurred in the federal district. in this study, hantavirus genomic sequences were amplified from seven patients who resided in central brazil and then sequenced and compared to other hantavirus sequences. the complete s segment sequence, which is 1847 bases long and potentially encodes the 428 amino acid nucleocapsid prote ...200919103306
predicting high risk for human hantavirus infections, increased risk for hemorrhagic fever with renal syndrome caused by puumala hantavirus was forecast for sweden in 2007. the forecast was based on a predicted increase in the number of myodes glareolus rodents (reservoir hosts). despite raised awareness and preparedness, the number of human cases during july 2007-june 2008 was 1,483, a new high.200919116065
ecology, genetic diversity, and phylogeographic structure of andes virus in humans and rodents in chile.andes virus (andv) is the predominant etiologic agent of hantavirus cardiopulmonary syndrome (hcps) in southern south america. in chile, serologically confirmed human hantavirus infections have occurred throughout a wide latitudinal distribution extending from the regions of valparaíso (32 to 33 degrees s) to aysén (46 degrees s) in southern patagonia. in this study, we found seropositive rodents further north in the coquimbo region (30 degrees s) in chile. rodent seroprevalence was 1.4%, with o ...200919116256
contact heterogeneity in deer mice: implications for sin nombre virus transmission.heterogeneities within disease hosts suggest that not all individuals have the same probability of transmitting disease or becoming infected. this heterogeneity is thought to be due to dissimilarity in susceptibility and exposure among hosts. as such, it has been proposed that many host-pathogen systems follow the general pattern whereby a small fraction of the population accounts for a large fraction of the pathogen transmission. this disparity in transmission dynamics is often referred to as ' ...200919129136
one for all? a viral protein supplants the mrna cap-binding complex. 200919129761
nephropathia epidemica with a 6-week incubation period after occupational exposure to puumala hantavirus.serological and molecular evidence showed that a german female student became infected by puumala hantavirus during mice trapping efforts in finland. the incubation period before exhibiting clinical signs of nephropathia epidemica was as long as 6 weeks. phylogenetic analysis of puuv nucleic acid sequences amplified from the patient demonstrate that she was infected by the puuv strain circulating at the place of her occupational exposure in finland.200919058998
hantavirus pulmonary syndrome in texas: 1993-2006.hantavirus pulmonary syndrome (hps) is a rare cardiopulmonary disease that was first described after a 1993 epidemic in the southwestern united states. this study reviewed all cases reported in texas to date.200919077779
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