TitleAbstractYear(sorted ascending)
the s rna segment of sandfly fever sicilian virus: evidence for an ambisense genome.the complete nucleotide sequence of the s rna segment of sandfly fever sicilian (sfs) virus (phlebovirus, bunyaviridae) was determined from overlapping cdna clones and by primer extension. the rna is 1746 nucleotides in length and has two large open reading frames (orf), one of which (24.8 kda) is viral-complementary in sense, and the other (30.4 kda) is in the viral sense. this ambisense genome arrangement has been seen in another member of the phlebovirus genus, punta toro (pt) virus (t. ihara ...19892705301
molecular characterization of the rna s segment of nephropathia epidemica virus strain hällnäs b1.the s segment rna of nephropathia epidemica virus (nev) strain hällnäs b1 was isolated by molecular cloning of the corresponding cdna. the rna is 1785 nucleotides long with the 3' and 5' termini being complementary for 23 bases. the viral messenger-sense rna contains one major open reading frame (orf) with a coding capacity of 433 amino acids encoding a 49-kda polypeptide. compared to the hantaan s segment cdna sequence there is a nucleotide homology of 60 and 61% at the amino acid level. many o ...19902104684
experimental infection of human vascular endothelial cells by pathogenic and nonpathogenic investigate whether the hantaviruses replicate in endothelial cells, we inoculated human umbilical vein endothelial cells with several pathogenic and nonpathogenic strains of hantavirus. intracytoplasmic, virus-specific granular fluorescence was detected initially at three days postinoculation and nearly 100% of cells contained viral antigen at 10 days. cytopathic effect or inclusion bodies were not observed. the in vitro demonstration of the susceptibility of endothelial cells to hantaviruse ...19902112908
[virus-specific proteins and rna of the virus of hemorrhagic fever with renal syndrome].virus-specific proteins g1, g2, and n with molecular weights of 70, 55-57, and 50 kilodaltons, respectively, were detected by radioimmunodiffusion tests in vero e-6 cells infected with strains of virus of hemorrhagic fever with renal syndrome (hfrs) isolated in the european ussr from a patient with hfrs, a fatal human case of hfrs (the strains k-27 and p-360) and from a bank vole (strain cg-1820). the sera from human convalescents after hfrs in the european ussr and rat sera prepared with the cg ...19902114054
nucleotide sequence of the m genome segment of hemorrhagic fever with renal syndrome virus strain b-1. 19902118626
hantavirus strains isolated from rodentia and insectivora in rural china differentiated by polymerase chain reaction assay.polymerase chain reaction (pcr) assay and other techniques were applied to differentiate hantavirus strains isolated from different animal hosts and geographic regions in china. two groups of related strains, hantaan and seoul, have been classified by cross-neutralization, radioimmunoprecipitation (rip), and sodium dodecyl sulfate polyacrylamide gel electrophoresis (sds-page) assays. the molecular weights of glycoprotein 1 (g1) of hantaan and seoul viruses were 72k and 80k, whereas those of the ...19902123383
zoonotic infections in northern ireland farmers.evidence of past zoonotic infection was investigated serologically in randomly selected northern ireland farmers. the percentage of farmers with antibody was: brucella abortus (0.7), leptospira interrogans serovars (8.1), borrelia burgdorferi (14.3), toxoplasma gondii (73.5), coxiella burnetii (28.0), chlamydia psittaci (11.1) and hantavirus (1.2). the results show that northern ireland farmers have been exposed in the past to zoonotic infections. it is not known if these infections contributed ...19902123457
nucleotide sequence of the l genome segment of hantaan virus. 19902123544
[study on natural infection of epidemic haemorrhagic fever virus in leptotrombidium scutellare].leptotrombidium (l.) scutellare was considered to be the suspected vector of epidemic haemorrhagic fever (ehf). in order to clarify the relationship between this mite and ehf, from oct to nov 1988 and oct of 1989, the larvae of this mite were collected from rats in areas with constantly high incidence of ehf in shanxi province and larvae with only small quantity of meal were selected and for more than 15 days before isolation of ehfv. six strains of virus were isolated. the viruses isolated were ...19902124535
incidence of antibodies against human immunodeficiency virus, human t-cell lymphotropic virus type 1, hepatitis b virus, hemorrhagic fever with renal syndrome virus and chlamydia in tonga and western samoa.among the populations of tonga and western samoa, serum antibodies against human immunodeficiency virus or hemorrhagic fever with renal syndrome virus were not detected (0/904 and 0/192). no serum samples were considered to be positive for antibody against human t-cell lymphotropic virus type 1 (0/527). hepatitis b antigen and antibody were found in 4% (8/192) and 47% (90/192), respectively. chlamydia trachomatis igg and c. psittaci igg antibodies were detected in 39% (75/192) and 47% (91/192), ...19902126048
diagnostic complementary dna probes for genome segments 2 and 3 of epizootic hemorrhagic disease virus serotype 1.potential diagnostic complementary dna (cdna) clones of gene segments 2 and 3 from epizootic hemorrhagic disease virus serotype 1 (ehdv-1) have been produced. individual segments of ehdv-1 were isolated, denatured with methylmercury hydroxide, and polyadenylated. the polyadenylated rna was reverse-transcribed and self-hybridized into duplex structures, and the incomplete ends were repaired. the resulting product was then cloned into the plasmid vector pbr322, using the complementary tailing meth ...19902164331
prevalence of antibody to hantavirus in some cat populations in britain. 19902177930
nucleotide sequence analysis of the s genomic segment of prospect hill virus: comparison with the prototype hantavirus.the genomic s rna segment of prospect hill virus (phv), a member of the hantavirus genus, was molecularly cloned and the nucleotide sequence of the cdna determined. the phv s rna segment is 1675 nucleotides long. a long open reading frame was identified in the viral complementary-sense rna that could encode a 433 amino acid (49k) nucleocapsid (n) protein. comparison with the sequence of the related hantavirus (hantaan 76-118) s rna segment indicated that there was 57% nucleotide sequence homolog ...19902309440
coding strategy of the s rna segment of dugbe virus (nairovirus; bunyaviridae).the s rna segment of dugbe (dug) virus (nairovirus; bunyaviridae) was sequenced from three overlapping cdna clones and by primer extension. the s rna is 1712 nucleotides in length and contains one large open reading frame (orf) of 1326 nucleotides coding for a 49.4-kda protein on viral complementary (vc) rna. this protein in size corresponds to the dug nucleocapsid (n) protein (p. cash, 1985, j. gen. virol. 66, 141-148). the 49.4-kda product was expressed as a fusion protein with beta-galactosid ...19902327076
rapid detection of genomic variations in different strains of hantaviruses by polymerase chain reaction techniques and nucleotide sequence analysis.the polymerase chain reaction (pcr) with subsequent nucleotide sequence analysis was employed to rapidly detect genomic variations among different hantavirus strains. using synthetic oligonucleotide primers derived from the m and s segment rnas of nephropathia epidemica virus strain hällnäs b1 (nev) we succeeded in amplifying the corresponding sequences of hantaan and puumala viruses. the nucleotide sequences of the cdnas derived from the puumala m and s rna segments were analyzed. it was found ...19902385957
[the use of the indirect fluorescent antibody technic for determining viral antigens in the urine of patients with hemorrhagic fever with renal syndrome].the authors suggest a new technique for the detection of viral antigen in the urine of hfrs patients, based on the method for the differential cytologic rapid diagnosis of acute respiratory viral diseases. hfrs virus antigen was detectable in the urine sediment epithelium in 7 of the 8 patients from the seventh to the thirty-second day of the follow-up. fluorescent bright-emerald dots could be seen in the urine sediment cells, situated on the cell surface, in the cytoplasm round the nucleus and ...19901695982
[the nature of the persistence of the causative agent of hemorrhagic fever with renal syndrome in the body of its natural host and its relationship to the epizootiologic and epidemic processes]. 19901699361
[nephropathia epidemica. hantavirus and acute renal insufficiency].puumala virus belongs to the hantavirus group and as other members of the group it can cause acute renal insufficiency. other hantaviruses are responsible for korean hemorrhagic fever and other hemorrhagic fevers with renal involvement. in scandinavia, hantavirus nephropathy is better known as nephropathia epidemica. hantavirus cause asymptomatic infections in mice and rats and the virus is thought to be transmitted to man via inhalation of desicated saliva, urine or feces from infected animals. ...19901967859
[hemorrhagic fever with renal syndrome]. 19901969359
[viral antigen localization and morphologic observation of the blood vessels in the skin of patients with hemorrhagic fever with early stage renal syndrome]. 19901969765
abnormalities of t cell immunoregulation in hemorrhagic fever with renal syndrome.the functions of spontaneous suppressor t cells and t lymphocyte subsets in patients with hemorrhagic fever with renal syndrome were compared. in the early stages of disease, decreased activity of spontaneous suppressor t cells was concurrent with increased numbers of cd8+ cells and a reversed cd4:cd8 ratio. these changes were related to abnormalities in serum c3 level and circulating immune complexes. in the recovery stages of the illness, spontaneous suppressor t cell activity and t cell subse ...19901969885
coding properties of the s and the m genome segments of sapporo rat virus: comparison to other causative agents of hemorrhagic fever with renal syndrome.three serologically distinct groups of hantaviruses have been associated with severe, moderate, and mild forms of hemorrhagic fever with renal syndrome (hfrs). to gain a better understanding of the genetic variation among these viruses, we cloned and sequenced the m and the s genome segments of sapporo rat virus, an etiologic agent of moderate hfrs, and compared the predicted gene products to those of hantaan virus, and the hällnäs strain of puumala virus, which are etiologic agents of severe an ...19901970443
infection with the puumala virus in pregnancy. case report. 19901970741
[electrolyte excretion in patients with a history of hemorrhagic fever with renal syndrome].renal transportation of sodium and potassium was studied in 85 patients who sustained hemorrhagic fever complicated with renal syndrome (hfrs). the examinees were given routine diet no. 7 recommended by pevzner. the conclusions were made on the analysis of circadian excretion and clearance of electrolytes studied and the levels of their water-load-induced tubular transportation: distal and proximal reabsorption and distal excretion of sodium, the correlation between potassium secretion and reabs ...19901970913
[the effect of hemodialysis on the vasopressin level and on the renin-aldosterone system in patients with hemorrhagic fever with renal syndrome].a total of 29 patients with severe hemorrhagic fever associated with the renal syndrome were studied for impact of extracorporeal hemodialysis on the activity of the renin-aldosterone system (ras), plasma vasopressin and osmolality and the levels of the major osmotically active agents, as well as the circadian urine output and blood pressures. in patients with oliguria there was a significant activation of ras, an increase in plasma vasopressin ad osmolality due to the increment of the urea in p ...19901970914
[hantaan virus infection--also in germany a cause of acute renal failure]. 19901971417
hemorrhagic fever with renal syndrome: mr imaging of the kidney.magnetic resonance (mr) imaging of the kidneys was performed in 14 patients with a severe form of hemorrhagic fever with renal syndrome (hfrs), a major public health problem in far eastern asia. to the authors' knowledge, this is the first report of renal assessment with mr imaging in hfrs. three mr imaging studies were done while patients were in the oliguric phase, 19 were done during the diuretic phase, and three were done during the convalescent phase. the authors differentiated the cortex a ...19901971450
hemorrhagic fever with renal syndrome. separation of human peripheral blood t and b cells and detection of viral this study, total blood lymphocytes were prepared from patients with hemorrhagic fever with renal syndrome (hfrs) by a density gradient on ficoll-hypaque. t and b cells were then purified by passing the total lymphocytes over a nylon wool column. the purities of total lymphocytes, t cells and b cells were 97.8 +/- 2.3%, 91.6 +/- 4.5% and 74.2 +/- 12.1%, respectively. also, after modification of cell fixation and smear drying, the number of cells were increased and the time needed for slide pr ...19901972048
antigenic subunits of hantaan virus expressed by baculovirus and vaccinia virus recombinants.baculovirus and vaccinia virus vectors were used to express the small (s) and medium (m) genome segments of hantaan virus. expression of the complete s or m segments yielded proteins electrophoretically indistinguishable from hantaan virus nucleocapsid protein or envelope glycoproteins (g1 and g2), and expression of portions of the m segment, encoding either g1 or g2 alone, similarly yielded proteins which closely resembled authentic hantaan virus proteins. the expressed envelope proteins retain ...19901972201
hemorrhagic fever with renal syndrome in a canadian serviceman. 19901972650
hantavirus infection in the united states: epizootiology and epidemiology.multiple species of murid and arvicolid (microtine) rodents serve as the reservoir hosts of hantaviruses, the etiologic agents of hemorrhagic fever with renal syndrome. three antigenically distinct hantaviruses have been isolated from rattus norvegicus, mus musculus, and microtus pennsylvanicus captured in the united states, and serologic evidence of a hantavirus enzootic has been found in several other indigenous rodent species. in residential districts of port cities such as baltimore, nearly ...19901972804
[characteristics of endemic foci of hemorrhagic fever with renal syndrome in various regions of the ussr].clinical, epizootological, and laboratory data for 9 endemic foci of hemorrhagic fever with renal syndrome (hfrs) were compared. the analysis covered 5120 cases of hfrs. it was established that in the foci, where the leading role is played by field mouse and japanese field mouse, hfrs is caused by the 1st serotype of hfrs virus, and the clinical course of the disease is quite severe. in the natural foci with the predominance of redbacked vole, the 2nd serotype of the virus circulates and the dis ...19901973011
[hemorrhagic fever with renal syndrome in lithuania]. 19901973012
[clinico-immunological characteristics of hemorrhagic fever with renal syndrome in khabarovsk territory]. 19901973013
[cases of hemorrhagic fever with renal syndrome in amur province associated with the rat serotype of the virus].to carry out serodiagnosis and to determine the serotype of the virus causing hemorrhagic fever with the renal syndrome (hfrs), paired sera obtained from 28 hfrs patients, 42 persons with a history of hfrs (1-17 years after convalescence), and 268 serum samples from healthy persons residing at the areas with the natural foci of this infection have been studied by indirect immunofluorescence techniques at the territory of the amur province. this study has demonstrated for the first time that, alo ...19901973327
outbreak of hemorrhagic fever with renal syndrome among u.s. marines in korea.fourteen of 3,754 u.s. marines who participated in a joint united states-republic of korea training exercise during the autumn of 1986 developed hemorrhagic fever with renal syndrome (hfrs). clinical and laboratory findings among cases included fever, headache, fatigue, gastrointestinal dysfunction, thrombocytopenia, and proteinuria. ten individuals were hospitalized; 2 died. no subclinical infections were identified through a post-deployment screen of sera obtained from 2,053 exercise participa ...19901973603
hemorrhagic fever with renal syndrome imported to hawaii from west germany. 19901974384
the protean manifestations of hemorrhagic fever with renal syndrome. a retrospective review of 26 cases from korea.twenty-six cases of hemorrhagic fever with renal syndrome from 1981 to 1986 were retrospectively reviewed to determine the scope of clinical presentation and the unique complications of the illness. the diagnosis was confirmed by detection of hantaan virus antibody in 25 cases and by characteristic autopsy findings in 1 case. the illness could be classified into three distinct clinical subgroups. fever was universally present. two patients presented with intractable shock and diffuse hemorrhage ...19901974404
hantavirus disease. 19901974945
[preliminary establishment of hybridoma secreting monoclonal anti-idiotypic antibodies to hemorrhagic fever with renal syndrome virus]. 19901975220
isolation of a strain of hantaan virus from peritoneal exudate cells of a patient with hemorrhagic fever with renal syndrome.a new strain of hantaan virus (htnv), gh716, was isolated from the peritoneal exudate cells (pec) of a patient with severe hemorrhagic fever with renal syndrome (hfrs). the isolate was propagated in vero e6 cells. at each passage the virus-infected cells were examined for htnv with immunofluorescence technique using monoclonal antibodies (mcab) 25-1 and 84-1 against htnv. from passage 5 on, fluorescent intensity tended to stabilize at (+++) and infectivity titer reached 10(5) tcid50/ml. using 14 ...19901976488
[the role of biologically active substances in permeability changes in the blood vessels of patients with hemorrhagic fever with renal syndrome].to judge the possible causes of the impairment of blood vascular permeability in hemorrhagic fever associated with the nephrotic syndrome (hfns), 86 patients were examined over time for the blood content of histamine, serotonin and kallikrein. the patients were found to have hyperhistaminemia, hyperserotoninemia and activation of blood serum kallikrein, which attests to undoubted participation of these biologically active substances in the pathogenesis of hfns.19901977202
[the thrombocyte link in the hemostasis of patients with hemorrhagic fever with renal syndrome].altogether 38 patients (33 men and 5 women) suffering from grave and moderate hemorrhagic fever with nephrotic syndrome (hfns) were examined. the hemolysate aggregation test and adp were employed to explore the aggregation ability of platelets in those patients. besides, measurements were made of the activity of the 3rd platelet factor in the plasma and serotonin content in each platelet. a considerable rise of the aggregation activity of platelets was established to take place in the oligoanuri ...19901977203
[vascular permeability, microcirculation and biologically active substances in patients with hemorrhagic fever with renal syndrome].as many as 56 patients with associated hemorrhagic fever and the renal syndrome were examined for vascular permeability, microcirculation and blood plasma histamine, serotonin, kallikrein and prekallikrein. in all the disease periods, the majority of the patients (n-52) demonstrated a rise of vascular permeability for protein and liquid part of the plasma, with this being marked in particular in the oligoanuric period. besides, significant perivascular and intravascular changes in microcirculati ...19901977204
[the cohort study on hfrsv antibody variation in normal population of an epidemic area].we randomly selected 2,796 persons in an epidemic area of hfrs in anhui province for serological study from april to december in 1988. the results showed that the antibody prevalence rates were 7.3%, 10% and 14% respectively in april, september and december. their gmt were 1.30, 1.42 and 1.73 respectively. indoor rodent density and hfrsv carrying rate were respectively 7.8%, 6.4% in april, 9.4%, 8.8% in september, and 7.5%, 8.2% in december. outdoor rodent density and hfrsv carrying rate were re ...19901977521
[the serological diagnosis of hantaan virus infections]. 19901977569
[the clinical picture and therapy of hantaan virus infection]. 19901977570
a retrospective analysis of sera collected by the hemorrhagic fever commission during the korean conflict.more than 600 sera from 245 patients with a clinical diagnosis of hemorrhagic fever were preserved by the hemorrhagic fever commission during the korean conflict, 1951-1954. these sera were tested for igm- and igg-specific antibodies to hantaan virus by enzyme immunoassay and for hantaviral antigen by immunoassay; one serum from each patient was tested by plaque reduction neutralization using both hantaan and seoul viruses. only 15 patients failed to develop antihantaviral antibodies; most sera ...19901977805
[epidemiology of hemorrhagic fever with renal syndrome in the ardennes region]. 19901978307
[hemorrhagic fever with renal syndrome in yugoslavia 1951-1988].data on hemorrhagic fever with renal syndrome (hfrs) incidence in sfry 1950-1988 were analysed. information sources were published papers and official reports of federal and republic institutions for public health. indirect immunofluorescence technique was used in testing 1.842 organs of small wild mammals to the presence of hfrs viral antigen. this antigen was found in the lungs of 11 species. average incidence of the carrier state was 10.4% registered were 613 cases within the period 1951-1988 ...19901978438
hantavirus infection presenting as acute renal failure. 19901978888
[hantaan virus serotypes circulating in foci of the far eastern region of the ussr].studies on serodifferentiation by indirect immunofluorescence antibody method and neutralization test of hantaan virus strains isolated from human patients and rodents revealed circulation on the maritime territory of 5 virus serotypes and demonstrated that rodents of one species could be virus carriers of different virus serotypes. on the basis of the antigenic similarity of strains isolated from human patients, field mice, and clethrionomys rufocanus it is concluded that the virus serotypes ci ...19901980045
different pathohistological presentations of acute renal involvement in hantaan virus infection: report of two cases.we present two patients with hantaan virus infection, admitted to the department of nephrology, skopje, at the same time, with the same clinical presentation (chills, fever, abdominal pain, hemorrhages, nausea, headache, proteinuria, hematuria, oliguria, acute renal failure) but with different pathohistological findings and different disease courses. in the first case diffuse proliferative glomerulonephritis was found, with a complete recovery of renal function after a month, with a mild protein ...19901980098
[the participation of histamine and serotonin in the genesis of acute kidney failure in patients with hemorrhagic fever with renal syndrome].in order to explore possible involvement of histamine and serotonin in acute renal failure related to hemorrhagic fever with renal syndrome (hfrs), selected markers or renal lesions have been compared with biochemical findings in highly severe cases. literature reports and the study results lead the authors to conclude that histamine and serotonin are obviously pathogenetic determinants of hfrs since their concentrations in plasma of these patients are increased 3-fold. it is suggested that the ...19901980387
[experiment research on moxibustion treating infected ehfv rat].in this experiment, we established a rat model of ehfv infected by abdominal cavity, and observed the effect of moxibustion on the changes of antigens, antibodies, neural transmitters in blood and tissue on 60 weanling wistar rats with ehf. the rats were divided into three groups: normal control group (n. group), ehf control group (c. group) and ehf with moxibustion group (m. group) at random. moxibustion was given at "shenshu" for 30 minutes daily, 7 days. the 14th day after infection of ehfv, ...19901980432
[epidemiologic characteristics of hemorrhagic fever with renal syndrome in a disease reservoir near foca]. 19901981115
[diagnosis of hemorrhagic fever with renal syndrome].the authors observed 22 patients with hemorrhagic fever with the renal syndrome (hfrs). the diagnosis turned out to be difficult in outpatient clinics as all the patients were referred to hospital with erroneous diagnoses. epidemiological causes of infection of hfrs were indicated. the authors singled out 3 periods in a course of this disease with characteristic symptoms. the diagnosis was confirmed serologically in the reaction of indirect immunofluorescence, a 4-fold increase and over in antib ...19901981246
hantavirus infection in laboratory and wild rodents in argentina.serum samples from urban and laboratory rats, laboratory mice and wild and laboratory cricetids in argentina were tested by immunofluorescence and plaque reduction neutralization tests to investigate prevalence of anti-hantavirus antibodies. a total of 102 sera were obtained from laboratory rodents in 4 different animal-rooms, 31 from harbor rats and 30 from wild cricetids in 1985-1987. anti-hantavirus antibodies were detected in 22.5% of rattus norvegicus in 3 of the animal-rooms but harbor rat ...19901981381
detection of antibodies to the western and the eastern type of haemorrhagic fever with renal syndrome in patient's sera from slovakia.we present serological evidence of infection with western and eastern type of haemorrhagic fever with renal syndrome (hfrs) virus in patient's sera from slovakia by indirect immunofluorescence. treatment of sera with 2-mercaptoethanol decreased levels of haemagglutination-inhibition (hi) antibodies to the eastern type of hfrs suggesting primary infection of the patients by this virus.19901981450
[the role of hantaan virus serotypes in the etiology of hemorrhagic fever with renal syndrome in the far east of the ussr].the employment of two serological tests (indirect fluorescence antibody technique and neutralization test) demonstrated the leading role of hantaan virus serotype 1 strains isolated from field mice in the pattern of the incidence of hemorrhagic fever with renal syndrome (hfrs). proofs of the etiological importance of strains of serotypes 3 and 5 occurring in brown rats and cl. rufocanus were obtained. no data on any association of human hfrs cases with strains of serotype 4 isolated from reed vo ...19901982032
haemorrhagic fever with renal syndrome: evaluation of elisa for detection of puumala-virus-specific igg and igm.igm and igg elisa to puumala virus were evaluated using sera from patients with haemorrhagic fever with renal syndrome (hfrs) from different geographical regions: sweden, denmark, norway, belgium and the european ussr. igm elisa proved useful in the diagnosis of hfrs in patients from all the regions mentioned above. specific igm could be detected as early as day 1 post onset of disease, and patients remained igm-positive for several months. specific igg elisa antibodies were also frequently dete ...19901982371
[spontaneous rupture of the kidneys in hemorrhagic fever with renal syndrome].during the recent 5 years we had 29 patients with hemorrhagic fever and renal syndrome complicated by a spontaneous kidney rupture. this complication occurred during an anuric period in 18 patients and within the first 24 hours of restored diuresis in 11 patients. major clinical presentations of the kidney rupture were deterioration of lumbar and abdominal pains, meteorism and symptoms of an internal bleeding. palpation revealed hematoma at the site of the ruptured kidney, tension of the anterio ...19901982474
[epidemiological survey of epidemic haemorrhagic fever in xianning county].the incidence, distribution pattern and trend of disease spreading of ehf in xianning country, hubei province during 1971-1985 are described. serologic study showed that the serum antigen positivity rate was 12.90% for apodemus agragrius and 6.67% for rattus norvegicus and that the serum antibody positivity rate was 61.54% in fhf patients and 30.85% in the exposed contacts of the patients' families in the epidemic area. in 1985, the hfrs virus a24 was isolated from apodemus agragrius and identif ...19901983075
[a ten-year retrospective investigation of serum against ehf antibodies in nanchong district, sichuan].this paper reports a retrospective inquiry of blood serum against epidemic hemorrhagic fever (ehf) antibodies in some of our hospital treated ehf patients during 1974-1984. it was found that the total positive rate of sera against ehf antibodies obtained from different periods of ehf patients was 86.8% (170/196). the titers were highest in patients who had been attacked within one year and the titers markedly decreased from the second year onwards. however certain level of serum titre could be d ...19901983076
[the use of nephrography in the differential diagnosis of epidemic nephritis]. 19901364680
primary structure of the large (l) rna segment of nephropathia epidemica virus strain hällnäs b1 coding for the viral rna polymerase.the l rna segment of the nephropathia epidemica virus (nev) strain hällnäs b1 was characterized by molecular cloning of the corresponding cdna and subsequent determination of the dna nucleotide sequence. the l rna segment is 6550 nucleotides long with complementarity of 20 bases at the 3' and 5' termini. the viral messenger sense rna contains one major open reading frame (orf) with a coding capacity of 2156 amino acid residues encoding a protein with a calculated molecular weight of 246 kda and ...19912053288
detection of hantavirus rna in tissues of experimentally infected mice using reverse transcriptase-directed polymerase chain reaction.detection of hantaviruses, the etiological agents of hemorrhagic fever with renal syndrome (hfrs), by virus isolation using experimental animals or cell culture is time-consuming. a more rapid but equally specific method is needed. we used a reverse transcriptase-directed polymerase chain reaction (rt-pcr) to detect hantavirus genomic sequences and compared its sensitivity with conventional virus isolation. rna, extracted by the guanidinium isothiocyanate-cesium chloride method from hantavirus-i ...19911713266
antigenicity of hantavirus nucleocapsid proteins expressed in e. coli.dna clones representing the small genomic segment of nephropathia epidemica virus strain hällnäs b1 (nev) and hantaan virus strain 76-118 (htv) encoding their nucleocapsid proteins were inserted into the e. coli vector pin-iii-ompa for secretion of proteins into the periplasmic space. the complete htv and nev nucleocapsid proteins and two truncated versions of the nev nucleocapsid proteins were expressed as fusion proteins. unexpectedly, all products accumulated as insoluble aggregates. most of ...19911714110
antigenic variation of european haemorrhagic fever with renal syndrome virus strains characterized using bank vole monoclonal antibodies.monoclonal antibodies (mabs) against puumala (puu) virus, the aetiological agent of nephropathia epidemica, were produced by fusing activated spleen cells from a bank vole (clethrionomys glareolus) with the mouse myeloma cell line sp2/0. this novel approach, utilizing the natural vector of puu virus for hybridoma production, proved to be highly efficient, and eight stable puu virus-specific heterohybridomas were isolated and characterized. the bank vole mabs were all specific for the nucleocapsi ...19911716651
antigenic variance. 19911718665
[differential diagnosis in nephropathia epidemica: nephrography or virus serology?]. 19911364468
[acute kidney failure and transient myopia. association suggestive of "hemorrhagic fever with renal syndrome"]. 19911828573
molecular characterization of the prospect hill virus m rna segment: a comparison with the m rna segments of other hantaviruses.complementary dna representing the genomic m rna segment of the prospect hill (ph) hantavirus was cloned and its nucleotide sequence determined. the ph virus m rna segment is 3707 nucleotides in length and has a long open reading frame in the viral complementary-sense rna with a coding capacity of 1142 amino acids. the predicted gene product of the ph virus m segment was compared with the corresponding gene products of hantaan virus strain 76-118 (hantaan), sapporo rat virus strain sr-11 (sr) an ...19911840609
nucleotide sequence and coding capacity of the large (l) genomic rna segment of seoul 80-39 virus, a member of the hantavirus genus.the nucleotide sequence of the large (l) genomic rna segment of seoul 80-39 virus was determined from overlapping cdna clones. the virion l rna segment is 6530 nucleotides long. the 3' and 5' terminal sequences are inversely complementary for 15 bases. the viral complementary-sense rna contains a single open reading frame from an aug codon at nucleotide position 37-39 to a uaa stop codon at nucleotide position 6490-6492. this orf could encode a polypeptide of 2151 amino acids (246,662 kda) which ...19911840713
experimental labyrinthitis in guinea pigs caused by a hantavirus.hantavirus-induced acute viral infection of the inner ear of guinea pigs is reported. guinea pigs were inoculated directly into the inner ear through the round window, or intraperitoneally with hantavirus (b-1 strain), and the cochlea was examined using an immunohistochemical technique for the localization of viral antigens. virus-specific antigens were detected prominently in the stria vascularis and to a lesser degree in other inner ear structures of the infected guinea pigs. the morphological ...19911901140
identification of hantavirus serotypes by testing of post-infection sera in immunofluorescence and enzyme-linked immunosorbent assays.serum samples were collected from 27 individuals who had been infected with a member of the genus hantavirus in the netherlands or belgium during the last 15 years. these samples were tested in an immunofluorescence assay (ifa) and two enzyme-linked immunosorbent assay (elisa) systems, using different virus strains that represented each of the four recently proposed serotypes of this genus. the serum samples from 11 individuals who had been infected through contacts with laboratory rats showed t ...19911901907
identification and characterization of a hantavirus strain of unknown origin by nucleotide sequence analysis of the cdna derived from the viral s rna segment.the genetic characterization of a serologically hantaan-like virus but of unknown origin (termed dx) was carried out by molecular cloning and nucleotide sequencing of the corresponding cdna of the viral s rna segment. the s rna was found to be 1765 nucleotides long with 3' and 5' termini being complementary for 24 bases. the virus messenger-sense rna contains one major open reading frame (orf) encoding 428 amino acids or a 50 kd polypeptide. a comparison of the dx s rna segment to those of sappo ...19911905433
stimulation of adherent cells by addition of purified proteins of viral hemorrhagic septicemia virus to trout kidney cell cultures.purified proteins of the virus causing viral hemorrhagic septicemia in the trout were added to cultures on semisolid medium of leukocytes obtained from either healthy or immunized rainbow trout. adherent cells were specifically stimulated by the glycoprotein of the viral spikes and, to a lesser extent, by the nucleoproteins. in contrast, a specific memory response was associated more with the nucleoproteins than with the glycoprotein when leukocytes from trout immunized with the virus were emplo ...19911905934
monoclonal antibodies to three strains of hantaviruses: hantaan, r22, and puumala.thirty hybrid cell lines that produce monoclonal antibodies to three strains of hantaviruses have been generated and characterized. one clone specific to hantaan 76-118 strain, four clones specific to rattus strains and one clone specific to puumala virus have been identified. most of the monoclones produced antibodies specific to nucleoproteins. only two monoclones were found to produce glycoprotein specific, neutralizing antibodies. the immunofluorescent (ifa) staining patterns of the monoclon ...19911907448
bunyaviridae: pathogenesis. 19911935228
bunyaviridae--natural history.obviously, the family bunyaviridae is comprised of a large number of epidemiologically diverse viruses. they vary dramatically in their vector and vertebrate host relationships, geographic distributions, and epidemic potential in humans and animals. public health practitioners, veterinarians, virologists, entomologists, biologists, ecologists, molecular biologists, and other scientists will all benefit from increased study and knowledge of this fascinating group of viruses.19911935229
hantaviruses in small wild living mammals in czechoslovakia. results of a 1983-1989 study.during a 7- year period were hunted small wild living mammals and examined by elisa and ria techniques for the presence of hantavirus antigen and/or antibodies by mfa. in total 3,050 animals of 16 species caught in 9 out of 10 regions of czechoslovakia, were examined. the proportion of positive animals was 4.4%. to the positive ones with the serotype 2 (western type) belonged the following: m. arvalis, c. glareolus, p. subterraneus, a. sylvaticus, a. flavicollis. to the eastern serotype: a. agra ...19911960391
molecular characterization and expression of glycoprotein gene of hantavirus r22 strain isolated from rattus norvegicus in china.a cdna containing the complete open reading frame of the m genome segment of hantavirus r22 strain isolated from rattus norvegicus in china, was amplified by polymerase chain reaction (pcr), and then cloned. the m segment is 3656 nucleotides in length with a predicted region of 3402 bases encoding a precursor glycoprotein of 1134 amino acids subsequently processed into viral glycoproteins 1 and 2 (g1 and g2). a strain comparison between r22 and sr11 (isolated from a rat in japan), and hantaan 76 ...19911962503
[hemorrhagic fever with renal syndrome--etiology].hfrs was confirmed serologically in two patients, who were infected in the natural focus near zupanja. using the bigger number of virus antigens in indirect immunofluorescent antibody test, afterwards was proved the agent could be classified into the serotype 3 of hantaviruses, and antigenically is identical with virus ne (strain hällnäs), respectively yugoslav isolate "vranica". serotype differentiation of the agent in patient who were infected in the area of igman, near sarajevo, has not been ...19911366321
a comparison of different genomic probes in the detection of virus-specified rna in orbivirus-infected cells.different 32p-labelled genomic probes of bluetongue virus (btv), epizootic haemorrhagic disease virus (ehdv) and equine encephalosis virus (eev) were compared with respect to the detection of virus-specified rna in infected cells. the probe derived from the genome segment that encodes nonstructural protein ns1 was found to be the most sensitive, detecting virus-specified rna in glutaraldehyde-fixed cells as early as 2-3 h p.i. this comparison was based on the observation that the ns1 gene probe ...19911651948
complete nucleotide sequence of segment 5 of epizootic haemorrhagic disease virus; the outer capsid protein vp5 is homologous to the vp5 protein of bluetongue virus.the complete nucleotide sequence of a cdna clone representing the segment 5 rna of epizootic haemorrhagic disease virus (ehdv) united states serotype 1 was determined. the 5' and 3' termini of the rna are complementary and are capable of forming secondary structures. the comparison of the predicted amino acid sequence of the encoded outer capsid protein (vp5) with the sequences of vp5 from four serotypes of bluetongue virus, the prototype orbivirus, revealed that the protein shares 59% to 62% ho ...19911662845
haemorrhagic fever with renal syndrome in germany. 19911670692
hantavirus infection and renal failure. 19911671905
different hantavirus serotypes in western europe. 19911671980
human-rodent contact and infection with lymphocytic choriomeningitis and seoul viruses in an inner-city population.we collected 1180 sera and 1363 questionnaires with information on demography, rodent exposure, and history of travel from persons visiting a sexually transmitted disease (std) clinic in baltimore between 1986-1988. serological tests for two rodent-borne viruses detected antibodies to lymphocytic choriomeningitis virus (lcmv) in 54 individuals (4.7%; n = 1149) and antibodies to seoul virus (seov) in three persons (0.25%; n = 1180). antibody prevalence to lcmv increased with age, but there were n ...19911672798
[hantaan virus infection]. 19911672850
[severe form of hemorrhagic fever with renal syndrome. an autochtonous case observed in parisian suburbs]. 19911673238
mechanisms of disease in hantavirus infection: pathophysiology of hemorrhagic fever with renal syndrome.hemorrhagic fever with renal syndrome (hfrs) is an acute viral disease that occurs over wide areas of europe and asia. hantaviruses are the cause of this syndrome. the hallmark of hfrs is the triad of fever, hemorrhage, and renal failure. in its severe form it is associated with significant mortality. the syndrome evolves through five phases: febrile, hypotensive, oliguric, diuretic, and convalescent. the central physiologic derangement in hfrs is vascular dysfunction, manifested by impaired vas ...19911673261
[hantaan virus infection in twente].to find out if hantavirus infection, as a cause of acute renal failure, has been overlooked in the past. to determine if hantavirus can be found in wild rodents, particularly in red bank voles (clethrionomys glareolus), living in the area.19911675769
specific puumala igg virus half a century after haemorrhagic fever with renal syndrome. 19911676130
precipitating antibodies to epizootic hemorrhagic disease and bluetongue viruses in white-tailed deer in the southeastern united states.from 1981 to 1989, sera were collected from 3,077 white-tailed deer (odocoileus virginianus) in georgia and from 1,749 deer from 12 additional states in the southeastern united states. in georgia, prevalence of precipitating antibodies to epizootic hemorrhagic disease virus (ehdv) and bluetongue virus (btv), as determined by agar gel immunodiffusion tests, was dependent on physiographic region, age, and year. overall prevalence of antibodies to ehdv and/or btv was 11, 33, 48, and 14% for the mou ...19911676762
rapid diagnosis of haemorrhagic fever with renal syndrome due to hantavirus. 19911677079
nephropathia epidemica in a patient with renal transplant. 19911677319
[a dynamic change of serotypes of hfrs patients in mixed epidemic area].in this paper, we have used hi assay in an observation of the dynamic changes of serotype of ehf patients in a mixed on demic area in shaoxing, zhejiang province. the results showed that serotypes of ehf patients were strongly correlated with different endemic areas and had obvious seasonal fluctuation in the incidence of the disease. this further demonstrates that the difference between the two serotypes (apodemus type and rattus type) in different geographic areas.19911677831
rapid diagnosis of epidemic nephritis caused by hantavirus. 19911678074
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