Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| aetiology of phlebotomus fever in ashkhabad studied in retrospect. | 1974 | 4156159 | |
| serological survey for antibodies to arboviruses in the human population of portugal. | 1974 | 4371206 | |
| antibodies against philebotomus fever viruses in human population of kuwait. | 1974 | 4376560 | |
| prevalence of antibodies to arboviruses in egypt. results of a serologic survey among 1,113 university students. | 1975 | 173201 | |
| multiplication of phlebotomus fever group arboviruses in mosquitoes after intrathoracic inoculation. | 1975 | 240026 | |
| transovarial transmission of viruses by phlebotomine sandflies. | 1975 | 197865 | |
| clinical and serologic responses of volunteers infected with phlebotomus fever virus (sicilian type). | twenty volunteers were inoculated with various doses of human serum containing phlebotomus fever virus (sicilian type) to determine their clinical and serologic responses as well as the human infectious dose50 of the virus. all infected subjects developed fever which varied in duration from 6 to 74 hours. the most common symptoms during sandfly fever were headache, anorexia, myalgia, photophobia, low back and retro-orbital pain. infected individuals developed a marked leukopenia characterized by ... | 1976 | 180844 |
| isolation of phlebotomus (sandfly) fever virus from sandflies and humans during the same season in aurangabad district, maharashtra state, india. | 1976 | 178593 | |
| incidence of arbovirus antibodies in bovine, ovine and human sera collected in eastern sicily. | the incidence of antibodies against 9 arboviruses in ovine, bovine and human sera collected in eastern sicily was studied. a high incidence of antibodies was found especially against bhanja virus in all sera and against sandfly fever sicilian in ovine sera only. the incidence was higher than that reported in 1971 for the western part of sicily. these findings suggest an increase in the circulation of arboviruses in this area; the causes are briefly discussed. | 1976 | 7946 |
| studies on the epidemiology of sandfly fever in iran. ii. the prevalence of human and animal infection with five phlebotomus fever virus serotypes in isfahan province. | human and animal sera from an endemic area of sandfly fever in iran were tested by plaque reduction neutralization method against five different phlebotomus fever virus serotypes (naples, sicilian, karimabad, salehabad, and i-47). the overall prevalence of naples, sicilian, and karimabad virus antibodies among the human population was 17%, 25%, and 66%, respectively. all sera were negative against salehabad and i-47 viruses. age-specific antibody rates suggested that sicilian and karimabad virus ... | 1977 | 192093 |
| studies on the epidemiology of sandfly fever in iran. i. virus isolates obtained from phlebotomus. | a total of 62 virus isolates was obtained from 12,485 male and female sandflies (predominately phlebotomus papatasi) collected in a village in central iran during the summer of 1975. the overall virus isolation rate from female sandflies was 1 per 177 insects processed. of the 62 virus strains obtained, 49 were identified as sicilian virus, 11 as karimabad, and 2 as a new member of the vesicular stomatitis serogroup. one isolate each of sicilian and karimabad virus was made from pools of male sa ... | 1977 | 192092 |
| effect of insecticide spraying for malaria control on the incidence of sandfly fever in athens, greece. | sera from 637 athens residents of various age groups were examined by plaque reduction neutralization test for antibodies against naples and sicilian phlebotomus fever viruses. a marked change in the prevalence of antibodies to both agents was observed in persons born after 1946, when residual insecticide spraying for malaria control was initiated in greece. the prevalence of naples and sicilian neutralizing antibodies among residents greater than or equal to 30 years of age was 36% and 13%, res ... | 1977 | 190909 |
| rio grande--a new phlebotomus fever group virus from south texas. | three strains of a new phlebotomus fever group virus were isolated from pack rats (neotoma micropus) collected in south texas during 1973--1974; the name rio grande was proposed for this virus. the virus is ph 3.0 labile, sensitive to the action of sodium deoxycholate and heat (56 degrees c) labile. the results of a serosurvey indicated that pack rats are probably the principal vertebrate host for rio grande virus and that year-round transmission of the virus may occur. because no isolations of ... | 1977 | 20785 |
| biochemical studies on the phlebotomus fever group viruses (bunyaviridae family). | analyses of the virion polypeptides and genomes of several phlebotomus fever group viruses, karimabad, punta toro, chagres, and the sandfly fever sicilian serotype viruses, have established that they are biochemically similar to the accepted members of the bunyaviridae family. like snowshoe hare virus (a member of the california serogroup of the bunyavirus genus of the bunyaviridae family), karimabad, punta toro, chagres, and the sandfly fever sicilian serotype viruses all have three viral rna s ... | 1979 | 225523 |
| dimethyl sulfoxide enhancement of phlebotomus fever virus plaque formation. | dimethyl sulfoxide (dmso)incorporated into an agar overlay containing deae-dextran enhanced plaque formation in vero cells by naples sandfly fever virus passaged in mouse brain or vero cell cultures. no plaques were visible when dmso was used without the deae-dextran, some plaques were rarely visible (less than 0.5mm) when deae-dextran was used without the dmso, and up to 10-fold more plaques were clearly visible (0.5-1.5 mm) when both chemicals were used. the combined enhancing effect of dmso a ... | 1979 | 223461 |
| phlebotomine sand flies (diptera: psychodidae) from a primary hill forest in west malaysia. | 1979 | 220422 | |
| serological relation between rift valley fever virus and viruses of phlebotomus fever serogroup. | 1980 | 6103252 | |
| bunyaviridae. | the family bunyaviridae comprises over 200 viruses (serotypes, subtypes, and varieties) that infect vertebrates and/or invertebrates. four genera of viruses have been defined (bunyavirus, nairovirus, phlebovirus, and uukuvirus). the main characteristics of the member viruses are: (i) the virus particles are for the most part uniformly spherical, 80-110 nm in diameter, and possess a unit membrane envelope from which protrude polypeptide spikes 5-10nm long; (ii) the viruses have three helical nucl ... | 1980 | 6165702 |
| diagnosis of viral infections by multicomponent mass spectrometric analysis. | metabolic profiles of urine extracts of humans with viral infections, as well as of media of virus-infected human tissue cultures, have been analyzed by non-fragmenting mass spectrometry and compared with corresponding controls. the spectra were then subjected to several alternative computerized statistical procedures to detect diagnostic biochemical profiles. controlled longitudinal studies on fully informed, consenting volunteers who received sandfly fever virus demonstrate the onset of a char ... | 1980 | 6250741 |
| the susceptibility of lutzomyia longipalpis (lutz and neiva), diptera, psychodidae, to artificial infection with three viruses of the phlebotomus fever group. | 1980 | 6257191 | |
| structural characteristics of nairoviruses (genus nairovirus, bunyaviridae). | viruses from six antigenic groups of arthropod-borne viruses [crimean--congo haemorrhagic fever (cchf), nairobi sheep disease (nsd), qalyub (qyb), sakhalin (sak), dera ghazi khan (dgk) and hughes (hug) serogroups], some previously categorized as bunyavirus-like viruses and others previously ungrouped, have recently been assembled by serological analyses into a new genus of viruses (nairovirus genus) in the bunyaviridae. molecular studies of the virion rna and viral polypeptides have been underta ... | 1981 | 7299367 |
| radioimmune assays and molecular studies that place anopheles b and turlock serogroup viruses in the bunyavirus genus (bunyaviridae). | molecular analyses indicate that turlock virus (tur, turlock serogroup) and boraceia virus (bor, anopheles b serogroup) have virion rna species and polypeptides comparable in size to those of members of the bunyavirus genus and unlike those of members of the newly defined phlebovirus, nairovirus, or uukuvirus genera (bunyaviridae). the 11 terminal 3' end nucleotides of the three virion rna species of both bor and tur viruses (houcaucacaug...) are identical in sequence to the 3' end sequences of ... | 1981 | 7258499 |
| studies on the antigenic relationship among phleboviruses. | 1982 | 6174048 | |
| experimental studies of rio grande virus in rodent hosts. | rio grande (rg) virus, a new member of the phlebotomus fever serogroup, was inoculated into wild wood rats (neotoma micropus) and laboratory-reared cotton rats (sigmodon hispidus) to determine if these potential hosts could be experimentally infected. nine of 14 (64%) wood rats became viremic, with titers of circulating virus ranging from 10(2.3) to 10(5.3) plaque-forming units (pfu)/ml and a geometric mean titer of 10(3.7) pfu/ml. virus was not detected in urine specimens from inoculated wood r ... | 1982 | 6282148 |
| antiviral efficacy of pyrazofurin against selected rna viruses. | the antibiotic pyrazofurin, 3-(beta-d-ribofuranosyl)-4-hydroxypyrazole-5-carboxamide, markedly inhibited the in vitro replication of a number of rna viruses including rift valley fever (rvf), venezuelan equine encephalomyelitis (vee), sandfly, pichinde, lassa and lcm virus. plaque formation was reduced by 80% or more with 2-10 micrograms/ml of pyrazofurin while 2 micrograms/ml reduced by 1000-fold the yield of lassa and lcm virus in a yield reduction assay. in vivo, pyrazofurin failed to protect ... | 1982 | 6299188 |
| morphogenesis of sandfly viruses (bunyaviridae family). | 1982 | 6287722 | |
| laboratory infection of the sandfly phlebotomus papatasi scopoli (diptera, psychodidae) with three phleboviruses. | membrane feeding and intrathoracic inoculation techniques were used to infect phlebotomus papatasi sandflies with phlebotomus fever group viruses. sandflies were orally infected with pacui virus, and were inoculated with pacui, sicilian sandfly fever and naples sandfly fever viruses. pacui virus did not multiply in these insects after oral infection, but an 8 x 10(4)-fold increase in virus concentration occurred within four days of intrathoracic inoculation of this virus. the infection rate was ... | 1983 | 6304951 |
| characterization of eight new phlebotomus fever serogroup arboviruses (bunyaviridae: phlebovirus) from the amazon region of brazil. | eight new members of the phlebotomus fever arbovirus serogroup (family bunyaviridae; genus phlebovirus) from the amazon region of brazil are described. one serotype was recovered from a febrile patient, three from small wild animals and four from sand flies. a small serum survey carried out with the human isolate, alenquer virus, suggests that it rarely infects man. complement-fixation and plaque reduction neutralization tests were done, comparing the eight new viruses with other members of the ... | 1983 | 6312820 |
| [use of the indirect hemagglutination inhibition reaction and of the radial hemolysis reaction in serological studies with the viruses of sandfly fevers]. | 1983 | 6314666 | |
| viruses isolated from panamanian sloths. | seven virus strains were isolated in vero cells from whole blood samples from 80 wild-caught sloths, bradypus variegatus and choloepus hoffmanni, from central panamá. four strains of at least two different serotypes are related to changuinola virus; two of these were associated with prolonged or recrudescent viremias. one strain is an antigenic subtype of punta toro virus, and another, described here as bradypus-4 virus, is a new, antigenically ungrouped virus. a second new virus from sloths, ut ... | 1983 | 6316795 |
| [arbovirus infections in tunisia: new serological survey of small wild mammals]. | sera of 103 small wild mammals (rodents, insectivora, cheiroptera) trapped at the beginning of 1980 in different areas of tunisia were studied for antibody against 15 arboviruses. in inhibition haemagglutination tests, positive reactions were found against dengue type 2 (2.2%), tahyna (18.4%), arumowot (42.7%) and sicilian sandfly fever (31%) viruses. no antibody was found for 8 tick-borne viruses. in comparison with previous sero-surveys, our results indicated a fall of west nile virus activity ... | 1983 | 6839405 |
| a sero-epidemiological survey for bunyaviridae and certain other arboviruses in pakistan. | complement fixation test reactions to the following viruses were studied in 372 samples (157 rodents, 172 domestic animals, 43 persons) from pakistan: bunyaviridae, phlebovirus: phlebotomus fever sicilian (pfs), phlebotomus fever naples (pfn), karimabad (kar), salehabad (sal); nairovirus: crimean-congo haemorrhagic fever (cchf), hazara (haz), dera ghazi khan (dgk), dhori (dho); uukuvirus: manawa (mwa); "possible members": bakau (bak), bhanja (bha). reoviridae, orbivirus: wad medani (wm). unclass ... | 1983 | 6415873 |
| transovarial transmission of rio grande virus (bunyaviridae: phlebovirus) by the sand fly, lutzomyia anthophora. | the growth of rio grande (rg) virus, the only phlebovirus known to occur in the united states, was studied in lutzomyia anthophora, its suspected sand fly vector. rg viral titers in infected flies increased more than 10,000-fold within 7 days after intrathoracic inoculation. experimentally infected female l. anthophora transmitted virus transovarially (vertically) to 54.8% of their f1 progeny. this is the first virologically confirmed demonstration of transovarial transmission of a phlebovirus b ... | 1983 | 6683943 |
| establishment and maintenance of persistent infection by the phlebovirus toscana in vero cells. | persistent infections were established by serial undiluted passages of the toscana virus of the genus phlebovirus in vero cells. persistence was maintained through more than 70 passages over a period of 2 years. the persistently infected cells were morphologically similar to the parental vero cells and released variable amounts of infectious virus. a small percentage of the persistently infected cells produced infectious virus while a larger percentage was shown to possess viral antigens by fluo ... | 1984 | 6693858 |
| [radial hemolysis reaction with the viruses of sandfly fever]. | 1984 | 6093388 | |
| serological studies on sandfly fevers in the republic of bangladesh. | blood samples from 160 inhabitants of the republic of bangladesh were studied by haemagglutination inhibition (hi), indirect hi, and radial haemolysis in gel tests. the sera were found to contain antibodies to the arborviruses of sicilian (6.25%) and naples (1.25%) sandfly fevers and to the karimabad virus (11.25%) which are transmitted by phlebotomus papatasi. antibodies to the karimabad virus were found among the bangladesh population for the first time. | 1984 | 6148858 |
| novel coding strategy (ambisense genomic rna) revealed by sequence analyses of punta toro phlebovirus s rna. | sequence analyses of punta toro viral s rna species indicate the existence of a novel coding strategy for rna viruses that involves both viral complementary and viral sense mrna species. the punta toro nucleocapsid protein (n, 26.9 x 10(3) da) is coded by a discrete viral complementary mrna species corresponding to the 3' half of the viral s rna. a second, presumably nonstructural, gene product (ns, 29.1 x 10(3) da) is coded by a viral sense mrna species that corresponds to the 5' half of the vi ... | 1984 | 6087547 |
| studies on the biology of phleboviruses in sand flies (diptera: psychodidae). i. experimental infection of the vector. | this paper describes a series of experiments which were done to determine the behavior of 14 different phleboviruses in laboratory-reared sand flies (phlebotomus papatasi, p. perniciosus and lutzomyia longipalpis) after oral and parenteral infection. most of the viruses replicated in the sand flies after intrathoracic inoculation; however, the insects were quite refractory to oral infection. six of 11 phleboviruses tested were transovarially transmitted in one or more sand fly species. the perce ... | 1984 | 6091466 |
| growth of the phlebovirus toscana in a mosquito (aedes pseudoscutellaris) cell line (ap-61): establishment of a persistent infection. | toscana virus, a sandfly-associated phlebovirus, was adapted to grow in cultured aedes pseudoscutellaris (ap-61) mosquito cell line. no evidence of virus growth was seen after primary infection of cell monolayers under maintenance conditions. on the contrary, persistent infections were established by subculturing infected cultures. cytopathic effect was never observed. significant titres of virus (10(3)-10(5) pfu/ml), as assayed in vero cells at 37 degrees c, were released from persistent infect ... | 1985 | 4015410 |
| growth of two phleboviruses after experimental infection of their suspected sand fly vector, phlebotomus perniciosus (diptera: psychodidae). | phlebotomus perniciosus were infected by intrathoracic inoculation and membrane feeding techniques with two phleboviruses (toscana and arbia) isolated in italy from this sand fly species. low levels of multiplication of both viruses were detected after intrathoracic inoculation of the sand flies. only some insects were found infected after oral ingestion of the two viruses. the percentage of flies infected orally was related to the amount of virus ingested. toscana virus was transovarially trans ... | 1985 | 3918472 |
| complete nucleotide sequence of the m rna segment of rift valley fever virus. | the entire m rna segment of the phlebovirus rift valley fever virus (rvfv) has been molecularly cloned and the complete nucleotide sequence determined. the rna is 3884 nucleotides in length, corresponding to a molecular weight of 1.38 x 10(6), having a base composition of 27.3% a, 25.4% g, 27.2% u, and 20.1% c. sequences present at the 3' and 5' termini of the molecule are largely complementary for some 51 residues and can form a stable duplex structure when the potential secondary structure of ... | 1985 | 2998042 |
| complete sequences of the glycoproteins and m rna of punta toro phlebovirus compared to those of rift valley fever virus. | the complete sequence of punta toro virus (phlebovirus, bunyaviridae) middle size (m), rna has been determined. the rna is 4330 nucleotides long (mol wt 1.46 x 10(6), base composition: 26.7% a, 33.6% u, 18.5% g, 21.2% c) and has 3'- and 5'-terminal sequences that, depending on the arrangement, are complementary for some 15 residues. the viral rna codes in its viral-complementary sequence for a single primary gene product (the viral glycoprotein precursor) that is comprised of 1313 amino acids (1 ... | 1985 | 2998043 |
| neurovirulent toscana virus (a sandfly fever virus) in swedish man after visit to portugal. | 1985 | 2860406 | |
| rift valley fever virus (family bunyaviridae, genus phlebovirus). isolations from diptera collected during an inter-epizootic period in kenya. | a total of 134 876 diptera collected in kenya during a 3-year period were tested in 3383 pools for rift valley fever (rvf) virus. nineteen pools of unengorged mosquitoes were found positive for rvf. all isolations were made from specimens collected at or near the naturally or artificially flooded grassland depressions that serve as the developmental sites for the immature stages of many mosquito species. the isolation of virus from adult male and female a. lineatopennis which had been reared fro ... | 1985 | 2862206 |
| analyses of the mrna transcription processes of punta toro phlebovirus (bunyaviridae). | the time course of the syntheses of punta toro (pt) phlebovirus (bunyaviridae) small (s)-size viral rna (s vrna), viral complementary rna (s vcrna), and messenger rna (s mrna) species has been analyzed using single-stranded dna probes representing the two s-coded gene products. the data obtained support the conclusion that pt s rna has an ambisense coding strategy (t. ihara, h. akashi, and d. h. l. bishop, virology 136, 293-306, 1984) with the viral nucleocapsid protein, n, encoded in a viral-co ... | 1985 | 2416115 |
| use of bacterial expression cloning to define the amino acid sequences of antigenic determinants on the g2 glycoprotein of rift valley fever virus. | four distinct antigenic determinants along the g2 glycoprotein encoded by the m segment rna of the phlebovirus rift valley fever virus were localized. these epitopes were defined by four monoclonal antibodies, three of which were capable of neutralizing virus infectivity; one was nonneutralizing. immunoprecipitation by these monoclonal antibodies of either denatured or native antigen characterized the epitopes as having linear or higher order structure. molecular cloning of g2 glycoprotein-codin ... | 1986 | 2422392 |
| ambisense rna viruses: positive and negative polarities combined in rna virus genomes. | the coding strategies of arenaviruses (family arenaviridae) and members of the phlebovirus genus of the bunyaviridae differ from those of other negative-sense rna viruses in that some proteins are coded in viral-complementary rna sequences and others are coded in the viral rna sequence. the term ambisense rna has been proposed to denote these unique coding arrangements. the implications of the ambisense rna coding strategy for the evolution and infection processes of these viruses are discussed. | 1986 | 3153160 |
| investigation of sandflies (diptera, phlebotomidae) in an endemic focus of visceral leishmaniasis in yugoslavia. | the paper presents the results of faunistic, ecological and viral investigations concerning phlebotomine sandflies in an endemic focus of visceral leishmaniasis in yugoslavia. these investigations were carried out in the period from 1969 to 1981. | 1986 | 3009286 |
| ambisense rna genomes of arenaviruses and phleboviruses. | 1986 | 3019106 | |
| [ecology and epidemiology of transmissible viruses of sandflies in italy]. | 1986 | 3019208 | |
| biology of arboledas virus, a new phlebotomus fever serogroup virus (bunyaviridae: phlebovirus) isolated from sand flies in colombia. | six isolates of a new phlebotomus fever serogroup virus, designated arboledas virus, were obtained from sand flies (lutzomyia spp.) collected in northeastern colombia. one of the isolates was made from a pool of male sand flies. by immunofluorescence, arboledas virus is related to caimito and pacui viruses; by neutralization test, it is distinct. arboledas virus neutralizing antibodies were found in the sera of opossums (didelphis marsupialis) and humans living in the study area. d. marsupialis ... | 1986 | 3024516 |
| messenger rna of the m segment rna of rift valley fever virus. | the putative messenger rna (mrna) of the m segment rna of the phlebovirus rift valley fever virus (rvfv) has been characterized using s1 nuclease mapping and oligonucleotide primer extension procedures. these experiments revealed that the 3' end of the mrna lacks approximately 112 nucleotides of the m genomic rna sequences, and that the 5' end of the mrna possesses all of the sequences present at the 3' end of the m rna but is further extended beyond the end of the genome by some 12-14 nucleotid ... | 1986 | 3962188 |
| comparative pathogenicity and antigenic cross-reactivity of rift valley fever and other african phleboviruses in sheep. | homologous and heterologous haemagglutination-inhibition (hai), complement-fixation (cf), immunodiffusion (id) and mouse neutralization tests were performed with the lunyo (lun) and a zimbabwean strain of rift valley fever (rvf) virus, the prototype and a south african strain of arumowot (amt) virus and prototype strains of gordil (gor), saint-floris (saf) and gabek forest (gf) viruses, using immune mouse ascitic fluids prepared against these viruses. reactions of identity occurred in all tests ... | 1986 | 3537119 |
| complete nucleotide sequence of the m rna segment of uukuniemi virus encoding the membrane glycoproteins g1 and g2. | we have determined the complete nucleotide sequence of the virion m rna segment of uukuniemi virus (uukuvirus genus, bunyaviridae) from cloned cdna. the rna that encodes the two membrane glycoproteins g1 and g2 is 3231 residues long (mol wt 1.1 x 10(6)). the 5' and 3' ends of the rna are partially complementary to each other for some 30 bp, enabling the formation of a stable panhandle structure (delta g = -40 kcal/mol) and the circularization of the molecule. the extreme 5' and 3' terminal nucle ... | 1987 | 3629974 |
| identification of the n and nss proteins coded by the ambisense s rna of punta toro phlebovirus using monospecific antisera raised to baculovirus expressed n and nss proteins. | an essentially complete dna copy of the ambisense s rna species of punta toro (pt) phlebovirus (t. ihara, h. akashi, and d.h.l. bishop, 1984, virology 136, 293-306) has been inserted in either orientation into autographa californica nuclear polyhedrosis baculovirus (acnpv) in lieu of the 5' coding region of the acnpv polyhedrin gene (g.e. smith, m.d. summers, and m.j. fraser, 1983, mol. cell. biol. 3, 2156-2165). the two types of recombinant viruses were used to infect spodoptera frugiperda cell ... | 1987 | 2950650 |
| maintenance of toscana virus in phlebotomus perniciosus by vertical transmission. | toscana virus was maintained in a laboratory colony of phlebotomus perniciosus by vertical (transovarial) transmission for 13 consecutive generations over a 23-month period. no significant biological changes were noted in the virus after prolonged vertical passage in the sand flies, and transovarially infected females were able to transmit the agent by bite to susceptible animals. chronic infection of ph. perniciosus with toscana virus had no apparent effect on the insects' rate of eclosion. in ... | 1987 | 3028194 |
| transmission of toscana virus by sandflies in italy. | 1987 | 2882214 | |
| viremia and immune response with sequential phlebovirus infections. | four groups of hamsters were infected sequentially with various combinations of arumowot, chagres, and gabek forest viruses. following each infection, the survival, level of viremia, and immune response of the animals were monitored. all of the agents produced viremia in the hamsters, regardless of the order of their administration. the antibody response, as measured by plaque reduction neutralization test, was monotypic even after two consecutive phlebovirus infections. arumowot and chagres vir ... | 1987 | 3034088 |
| sand fly fever-naples infection in egypt. | two egyptian male patients with sand fly fever-naples virus infection are presented. the virus was isolated from one patient while both patients had diagnostic rises in indirect fluorescent antibody titers to the virus. the viral isolate, sfn 85055, grows to much higher titers and plaques more efficiently than the prototype sand fly fever-naples virus and should facilitate work with this virus. | 1987 | 3037932 |
| sandfly fever epidemiology in croatia. | 1987 | 3039802 | |
| immunoelectron microscopy of rift valley fever viral morphogenesis in primary rat hepatocytes. | the morphogenesis of the hepatotropic phlebovirus rift valley fever virus (rvfv) has been examined by immuno-electron microscopy in primary hepatocyte cultures derived from genetically susceptible and resistant rat strains. rvfv replicates in both cell types with growth kinetics comparable with those seen in other permissive cells. however, in contrast to that has been observed in other cell types, rvfv replication in hepatocytes is associated with maturation at cellular surface membranes in add ... | 1987 | 3499704 |
| punta toro virus infection of c57bl/6j mice: a model for phlebovirus-induced disease. | punta toro virus infections of inbred strains of mice have been characterized and evaluated as a model in which to study various aspects of the host response to phlebovirus infections and the requirements for protective immunity. the adames strain of punta toro virus was found to be strongly hepatotropic and lymphotropic and the outcome of infection was largely a function of age. c57bl/6j mice of less than 5 weeks of age uniformly developed fulminant hepatocellular necrosis with mean survival ti ... | 1987 | 2462155 |
| characterization of punta toro s mrna species and identification of an inverted complementary sequence in the intergenic region of punta toro phlebovirus ambisense s rna that is involved in mrna transcription termination. | the transcription termination sites for the subgenomic n and ns(s) mrna species coded by the 1904 nucleotide, ambisense s rna species of punta toro (pt) phlebovirus (bunyaviridae, ihara et al., 1984) have been identified by northern analyses using a series of synthetic oligodeoxynucleotides. for the viral-complementary n mrna species the oligonucleotides that were used represented viral rna sequences; for the viral-sense ns(s) mrna species they represented viral-complementary sequences. the resu ... | 1987 | 18644551 |
| antigenic analysis of punta toro virus and identification of protective determinants with monoclonal antibodies. | hybridomas producing monoclonal antibodies to the three major structural proteins of punta toro virus (ptv) were established by fusion of spleen cells with sp2/0-ag-14 mouse plasmacytoma cells. thirty-six independently derived monoclonal antibodies were evaluated in neutralization, hemagglutination inhibition, and elisa assays and the isotype, antigen specificities, and cross-reactivities were determined. these antibodies were also assessed for their ability to provide protection in a murine mod ... | 1988 | 2462308 |
| [rift valley fever and phleboviroses in the central african republic]. | during 1984 and 1985, six rift valley fever virus strains (rvf) were isolated in central african republic, among them five from human samples. three strains were isolated in 1985 at the end of the rainy season, from sera of patients dead with severe jaundice with haemorrhagic syndrome, what could evoke a little outbreak. at this occasion, these rvf strains and the other strains of phleboviruses previously isolated in car, were antigenically compared by immuno-fluorescent assay (ifa) and compleme ... | 1988 | 3042177 |
| rift valley fever virus m segment: cellular localization of m segment-encoded proteins. | the phlebovirus rift valley fever virus (rvfv), like other members of the bunyaviridae family, matures intracellularly at the smooth-surfaced vesicles in the golgi region of infected cells. here we show that in cultured cells the rvfv glycoproteins g2 and g1 accumulate and are retained at this site. to investigate the parameters governing this subcellular localization, we have engineered portions of the cloned rvfv m segment (which encodes a 14- and a 78-kda protein, in addition to glycoproteins ... | 1988 | 3046119 |
| ecology of viruses isolated from sand flies in italy and characterized of a new phlebovirus (arabia virus). | a total of 84 virus strains was obtained from 16,374 male and female sand flies (phlebotomus perniciosus and p. perfiliewi) collected in two localities of tuscany region in italy between 1980 and 1985. thirty-seven (44%) were identified as toscana virus (family bunyaviridae, genus phlebovirus) and 47 (56%) as a new member of the phlebotomus fever serogroup, arbia virus. the characteristics of this new serotype are described. the overall virus isolation rate from sand flies was 0.5 per 100 insect ... | 1988 | 3128131 |
| in vitro and in vivo phlebovirus inhibition by ribavirin. | ribavirin (1-beta-d-ribofuranosyl-1,2,4-triazole-3-carboxamide) was markedly inhibitory in vitro to adames and balliet strains of punta toro virus (ptv), a phlebovirus related to rift valley fever and sandfly fever viruses. by using inhibition of viral cytopathic effect in llc-mk2 cells with both virus strains, the 50% effective dose was 4 to 10 micrograms/ml and the virus rating was 1.3. the adames strain of ptv infection in mice was established for evaluation of the in vivo antiviral efficacy ... | 1988 | 3129991 |
| [virus transmission to man from phlebotomus flies: role of the tuscany virus (bunyaviridae, phlebovirus) in the etiology of infections of the central nervous system]. | during field studies on the ecology of arboviruses in italy, strains of a new virus were isolated from sand flies, mostly females, of a pure colony of phlebotomus perniciosus collected in a central italian region (tuscany). the prototype was named toscana (tos) virus, assigned to the phlebotomus fever serogroup of arboviruses (family bunyaviridae, genus phlebovirus) and registered in 1980 in the international catalogue of arboviruses. neutralizing antibodies to tos virus were detected in human s ... | 1988 | 3152279 |
| molecular and biochemical studies of the evolution, infection and transmission of insect bunyaviruses. | members of the bunyaviridae family of rna viruses (bunyaviruses, hantaviruses, nairoviruses, phleboviruses and uukuviruses) have been studied at the molecular and genetic level to understand the basis of their evolution and infection in vertebrate and invertebrate (arthropod) hosts. with the exception of the hantaviruses, these viruses infect and are transmitted by a variety of blood-sucking arthropods (mosquitoes, phlebotomines, gnats, ticks, etc.). the viruses are responsible for infection of ... | 1988 | 2907153 |
| rift valley fever virus m segment: cell-free transcription and translation of virus-complementary rna. | a cell-free system has been used to study gene expression of the m segment rna of the phlebovirus rift valley fever virus (rvfv). rvfv sequence-containing plasmids were used to synthesize m segment mrna-like transcripts. these transcripts were then translated in vitro in the absence or presence of microsomal membranes. cell-free translation of a transcript which closely resembled authentic m segment mrna (rna-7) yielded a primary translation product of 133 kilodaltons (kda), the size expected of ... | 1988 | 3285574 |
| the genus phlebovirus and its vectors. | 1988 | 2829707 | |
| experimental infection of phlebotomus papatasi with sand fly fever sicilian virus. | experimental studies were conducted to evaluate humans as hosts infecting the sand fly phlebotomus papatasi with sand fly fever sicilian (sfs) virus. viral antigen and infectious virus circulated in the blood of infected volunteers on days 4 and 5 after intravenous inoculation with sfs virus. viremia levels during the latter period were high enough to infect feeding sand flies, but only 13% (9/69) of the flies became infected. one out of every 3 infected sand flies that survived to feed a second ... | 1988 | 2849886 |
| rift valley fever virus m segment: use of recombinant vaccinia viruses to study phlebovirus gene expression. | recombinant vaccinia viruses were constructed and used in conjunction with site-specific antisera to study the coding capacity and detailed expression strategy of the m segment of the phlebovirus rift valley fever virus (rvfv). the m segment could be completely and faithfully expressed in recombinant rvfv-vaccinia virus-infected cells, the gene products apparently being correctly processed and modified in the absence of the rvfv l and s genomic segments. the proteins encoded by the rvfv m segmen ... | 1988 | 3339714 |
| effects of ribamidine, a 3-carboxamidine derivative of ribavirin, on experimentally induced phlebovirus infections. | ribamidine (1-beta-d-ribofuranosyl-1,2,4-triazole-3-carboxamidine) was inhibitory in rhesus monkey kidney (llc-mk2 derivative) cells to adames and balliet strains of punta toro virus (ptv), a phlebovirus related to rift valley fever and sandfly fever viruses. the 50% effective dose was 8 and 12 micrograms/ml against each respective virus strain; the 50% cytotoxic dose was 320 micrograms/ml, giving selectivity indices of 40 and 27 against each virus strain. the virus ratings were 1.2 and 1.0, res ... | 1988 | 2976263 |
| experimental studies on the maintenance of toscana and arbia viruses (bunyaviridae: phlebovirus). | transovarial transmission (tot) of toscana (tos) and arbia (arb) viruses in a laboratory colony of phlebotomus perniciosus is reported. toscana and arb viruses were maintained in p. perniciosus females, initially infected by intrathoracic inoculation, for 2 and 3 consecutive generations respectively. tot was demonstrated in f1 (75%) and f2 (67%) generation adults for tos and f1 (47%), f2 (37%), and f3 (34%) generation adults for arb virus. the progressive decline of virus infection rates in each ... | 1989 | 2545100 |
| development of an enzyme linked-immunosorbent assay (elisa) for the sand fly fever viruses detection. | an enzyme linked-immunosorbent assay (elisa) was recently established for the detection of sandfly naples and sicilian viruses (sfn and sfs) from laboratory infected as well as wild caught sandflies in egypt. optimal dilutions of the reactants including the coating antibodies (rabbit antiserum), detecting antibodies (mouse antiserum), conjugate and the time used for incubation of the substrate (abts) were determined for both sfn and sfs viruses. the elisa test showed to be highly specific and se ... | 1989 | 2562441 |
| pathogenesis of viral hemorrhagic fevers: rift valley fever and lassa fever contrasted. | although many viral infections have on occasion been associated with hemorrhagic complications, infection with any of several rna viruses regularly results in vascular involvement and the syndrome called viral hemorrhagic fever (vhf). in spite of clinically useful similarities among various vhfs, there are significant differences in their pathogenesis and clinical evolution; these are often related to characteristics of their viral taxon. infection with rift valley fever (rvf) virus, a phlebovir ... | 1989 | 2665011 |
| the s rna segment of sandfly fever sicilian virus: evidence for an ambisense genome. | the complete nucleotide sequence of the s rna segment of sandfly fever sicilian (sfs) virus (phlebovirus, bunyaviridae) was determined from overlapping cdna clones and by primer extension. the rna is 1746 nucleotides in length and has two large open reading frames (orf), one of which (24.8 kda) is viral-complementary in sense, and the other (30.4 kda) is in the viral sense. this ambisense genome arrangement has been seen in another member of the phlebovirus genus, punta toro (pt) virus (t. ihara ... | 1989 | 2705301 |
| rift valley fever virus m segment: phlebovirus expression strategy and protein glycosylation. | the m segment rna of rift valley fever virus (rvfv) encodes four gene products: the two viral envelop glycoproteins g2 and g1, a glycosylated 78-kda protein, and a nonglycosylated 14-kda protein. these proteins are generated from a single open reading frame (orf) by a strategy involving independent translational initiations at both the first and second in-phase atg codons and co-translational processing of primary polyprotein products. the orf encodes six sites for n-linked glycosylation: one pr ... | 1989 | 2728348 |
| [epidemiology of rift valley fever in western africa. i. serologic survey in domestic ruminants of burkina faso]. | a serosurvey of rift valley fever virus infection has been conducted among 2,410 domestic ruminants (sheep, goats and cattle) from burkina faso in 1985, 1986 and 1987. an active circulation of virus was demonstrated and 26.7% of the total animal tested had rift valley fever virus reacting antibodies. an epizootic was demonstrated retrospectively for 1987 and appears to correspond to the rvf outbreak observed in the same type of sahelian environment during the same year in mauritania. analogous e ... | 1989 | 2766443 |
| characterization of five new phleboviruses recently isolated from sand flies in tropical america. | five new phlebotomus fever virus serotypes (bunyaviridae: phlebovirus) are described. these viruses, designated ambe, ixcanal, mariquita, armero, and durania, were isolated from sand flies (diptera: psychodidae) collected in brazil, colombia, and guatemala. two of the agents were recovered from pools of male sand flies. the new viruses are antigenically related to other members of the phlebotomus fever serogroup by immunofluorescence, but are distinct from the other 39 members of this serogroup ... | 1989 | 2543227 |
| infection and coding strategies of arenaviruses, phleboviruses, and nairoviruses. | the infection and coding strategies of three groups of negative-stranded rna viruses (arena viruses, phleboviruses, and nairoviruses) that include the etiologic agents of hemorrhagic disease in humans have been studied. arenaviruses have two viral rna species. the smaller rna species (s) codes for the viral nucleoprotein (n protein) and for the viral glycoprotein species (g1 and g2, which are derived from a precursor glycoprotein, gpc). the s rna has an ambisense arrangement. the proteins are tr ... | 1989 | 2546246 |
| [development and use of immunoenzyme analysis for diagnosing sandfly fever]. | 1989 | 2555967 | |
| arbovirus infections and viral haemorrhagic fevers in uganda: a serological survey in karamoja district, 1984. | sera collected in may 1984 from 132 adult residents of karamoja district, uganda, were examined by haemagglutination inhibition tests for antibodies against selected arboviruses, namely chikungunya and semliki forest alphaviruses (togaviridae); dengue type 2, wesselsbron, west nile, yellow fever and zika flaviviruses (flaviviridae); bunyamwera, ilesha and tahyna bunyaviruses (bunyaviridae); and sicilian sandfly fever phlebovirus (bunyaviridae); and by immunofluorescence tests against certain hae ... | 1989 | 2559514 |
| viruslike particles in liver in sporadic non-a, non-b fulminant hepatitis. | in a patient who followed the typical clinical course of fulminant hepatitis attributable to "sporadic" non-a,non-b (nanb) hepatitis and who finally received treatment by orthotopic liver grafting, three, apparently separate, virus-like agents (26, 45, and 80 nm) and cytoplasmic, reticular tubular structures (cts) were identified in collapsed and regenerating areas of liver using electron microscopy. the 80-nm particles present within vacuoles, together with the finding of intranuclear rods in a ... | 1989 | 2493513 |
| [the etiology of sandfly fever in soviet servicemen in afghanistan]. | 1989 | 2541564 | |
| incidence of sand fly fever among swedish united nations soldiers on cyprus during 1985. | members of a swedish united nations (un) battalion of 362 soldiers were bled just before and immediately after their 6 month tour of duty in cyprus during 1985. sera were tested for presence of specific antibodies to sand fly fever (sf) sicilian, sf naples, and toscana viruses by both indirect immunofluorescence antibody test (ift) and plaque reduction neutralization test (prnt). of 298 serum pairs available, seroconversion was noted in 11 soldiers: 7 cases for sf sicilian, 3 for sf naples, and ... | 1990 | 2167616 |
| expression strategy of a phlebovirus: biogenesis of proteins from the rift valley fever virus m segment. | the middle (m) rna segment of rift valley fever virus (rvfv) encodes four proteins: the major viral glycoproteins g2 and g1, a 14-kilodalton (kda) protein, and a 78-kda protein. these proteins are derived from a single large open reading frame (orf) present in the virus-complementary m-segment mrna. we used recombinant vaccinia viruses in which sequences representing the m-segment orf were engineered as a surrogate system to study phlebovirus protein expression. to investigate the translational ... | 1990 | 2319645 |
| coding strategy of the s rna segment of dugbe virus (nairovirus; bunyaviridae). | the s rna segment of dugbe (dug) virus (nairovirus; bunyaviridae) was sequenced from three overlapping cdna clones and by primer extension. the s rna is 1712 nucleotides in length and contains one large open reading frame (orf) of 1326 nucleotides coding for a 49.4-kda protein on viral complementary (vc) rna. this protein in size corresponds to the dug nucleocapsid (n) protein (p. cash, 1985, j. gen. virol. 66, 141-148). the 49.4-kda product was expressed as a fusion protein with beta-galactosid ... | 1990 | 2327076 |
| the occurrence of ephemeral fever in kenya, 1968-1988. | ephemeral fever occurs in kenya in epidemics which have been associated with periods of unusually heavy and prolonged rainfall. the epidemics persist for 2-3 years and involve most of the country. they occur in the same years as epizootics of rift valley fever, which is a mosquito-borne phlebovirus infection. sporadic outbreaks of the disease have been associated with local heavy rains; though one outbreak occurred during a dry period. culicoides spp. were generally present in large numbers at t ... | 1990 | 2353443 |
| ambisense segment 4 of rice stripe virus: possible evolutionary relationship with phleboviruses and uukuviruses (bunyaviridae). | the complete nucleotide sequence of segment 4 of the rice stripe virus (rsv) genome was determined from overlapping cdna clones and by direct rna sequencing. the segment has two long open reading frames (orfs). one of the orfs (534 bases) is in the 5' region of the viral sequence, and the other (858 bases) is in the viral complementary sequence of the viral 3' region. such ambisense genome organizations have been seen previously in rnas of phleboviruses, uukuviruses and arenaviruses. the orf in ... | 1990 | 2374003 |
| pathogenesis of a phleboviral infection (punta toro virus) in golden syrian hamsters. | the hamster, mesocricetus auratus, was examined as a possible model for investigating the poorly defined pathogenesis of the family bunyaviridae, genus phlebovirus. punta toro virus (ptv) isolates from eastern panama were highly virulent for two outbred and five inbred hamster strains, while isolates from western panama were of low virulence. the adames strain (eastern panama) of ptv (ld50 approximately 1 pfu, sc) caused an acute fatal disease (average survival time, 3.8 days) in 10-week-old lak ... | 1990 | 2122875 |
| uukuniemi virus s rna segment: ambisense coding strategy, packaging of complementary strands into virions, and homology to members of the genus phlebovirus. | we determined the complete nucleotide sequence of the small (s) rna segment of uukuniemi virus, the prototype of the uukuvirus genus within the bunyaviridae family. the rna, which is 1,720 nucleotides long, contains two nonoverlapping open reading frames. the 5' end of one strand (complementary to the viral strand) encodes the nonstructural protein nss (273 residues; molecular weight, 32,019), whereas the 5' end of the viral-sense strand encodes the nucleocapsid protein n (254 residues; molecula ... | 1990 | 2136709 |
| neutralizing antibodies for sandfly fever naples virus in human sera on the island of mljet. | sera from apparently healthy residents of various age from different localities of the adriatic island of mljet were tested by the plaque reduction neutralization test (prnt) for antibodies to sandfly fever naples virus (sfn). an average of 51.4% of the sera examined was found to have neutralizing antibodies. reactors to sfn virus were found in all the age groups examined and the antibody prevalence appeared to increase with age. the presence of sfn neutralizing antibodies in all age groups indi ... | 1990 | 2159683 |
| [virological study of cases of sandfly fever in afghanistan]. | three strains (af-1008, af-1038, and af-130) of neapolitan sandfly fever virus (nsff) and 2 strains (af-1028 and af-83) of sicilian sandfly fever virus (ssff) were isolated from febrile patients among soldiers of the limited military contingent of soviet troops in afghanistan in may-august, 1986-1987. the viruses were isolated in neonate mice and identified by indirect immunofluorescence, complement-fixation tests and plaque reduction neutralization test in vero e6 and spev cells. the new nsff s ... | 1990 | 2163568 |
| the s rna segment of tomato spotted wilt virus has an ambisense character. | the complete nucleotide sequence of the s rna of tomato spotted wilt virus (tswv) was determined. the rna is 2916 nucleotides long and has an ambisense coding strategy. the sequence contains two open reading frames (orfs), one in the viral sense which encodes a protein with a predicted mr of 52.4k and one in the viral complementary sense which encodes the viral nucleocapsid protein of mr 28.8k. both proteins are expressed by translation of two subgenomic rna species that possibly terminate at a ... | 1990 | 1693160 |
| prophylactic and therapeutic activities of 7-thia-8-oxoguanosine against punta toro virus infections in mice. | the biological response modifier 7-thia-8-oxoguanosine was evaluated in mice against the hepatotropic adames strain of punta toro virus. when administered intraperitoneally in divided doses, significant protection from death was conferred at doses of 50 and 100 mg/kg/day given 24 and 17 h pre-virus inoculation, 25-100 mg/kg/day administered 4 h pre- and 3 h post-virus challenge, and 12.5 to 100 mg/kg/day administered 24 and 31 h after virus inoculation. these doses preventing death reduced liver ... | 1991 | 1716090 |
| antigenic variance. | 1991 | 1718665 | |
| [epidemiology of rift valley fever in west africa. 1. serological investigation of small ruminants in niger]. | a serosurvey of rift valley fever virus infection conducted among 557 sheep and 643 goats from niger in 1986 points out that 2.8% of the 1,200 animals tested had rvf virus reacting antibodies. the circulation of the virus is demonstrated, as well for another phlebovirus related to rvf virus, the strain arumowot. | 1991 | 1764749 |