| tryptic peptide analysis of the structural proteins of vesicular stomatitis virus. | the individual structural polypeptides of vesicular stomatitis virus have been examined by tryptic peptide analysis of 35s-methionine preparations labelled in vivo and 125i-preparations labelled in vitro. isolates of the two classical serotypes of the virus (indiana and new jersey) and of a sub-type of the indiana serotype, brazil virus, were compared. the study showed that the major internal proteins of all three viruses gave similar maps, whereas the surface glycoproteins gave distinct maps th ... | 1978 | 203655 |
| interferon action iii. the rate of primary transcription of vesicular stomatitis virus is inhibited by interferon action. | transcription of vesicular stomatitis virus (vsv) in vero cells was confined to the synthesis of parentally-derived mrna (primary transcription) by the use of cycloheximide and/or a ts mutant, g41(iv), at a non-permissive temperature (40 degrees c). more transcripts accumulated in the presence of cycloheximide than in its absence. this so-called "cycloheximide effect" results from higher rates of virus transcription sustained for longer periods of time. the rate of vsv transcription initially in ... | 1978 | 204729 |
| in vitro synthesis of vesicular stomatitis virus membrane glycoprotein and insertion into membranes. | translation in vitro of the mrna coding for the vesicular stomatitis virus membrane glycoprotein g in a membrane-free ribosomal extract from hela cells allowed the synthesis of only the unglycosylated protein g1 (molecular weight, 63,000). addition of stripped crude microsomal membranes from hela cells resulted in the conversion of g1 to the glycosylated protein g2 (molecular weight, 67,000). the g2 protein synthesized by the reconstructed microsomal membrane/ribosome system was found to be segr ... | 1978 | 204929 |
| complete sequences of the ribosome recognition sites in vesicular stomatitis virus mrnas: recognition by the 40s and 80s complexes. | nucleotide sequences of the ribosome-protected translation initiation sites from the vesicular stomatitis virus (vsv) m and l protein mrnas have been determined, completing the sequences of the sites from all the vsv mrnas. a low level of protection at two internal aug-containing sites in the n mrna is also described. small homologies are evident among some of the sites, but there are no obvious features common to all the sites other than a single aug codon. in contrast, a large homology between ... | 1978 | 208778 |
| carbohydrate structure of vesicular stomatitis virus glycoprotein. | | 1978 | 209045 |
| size distributions of vesicular stomatitis virus-specific polysomes. | | 1978 | 205627 |
| detection of vesicular stomatitis virus (murine leukemia virus) pseudotypes by immunoelectron microscopy. | | 1978 | 209619 |
| comparative studies on cytopathic effects induced by vesicular stomatitis virus in two cell types. | infection of mouse l cells with vesicular stomatitis virus (vsv) leads to an extensive cell fusion, while porcine kidney stable (ps) cells infected with vsv show only cell rounding. therefore, comparative morphological studies on the infection of the two cell lines were carried out using a transmission or scanning electron microscope and an immunofluorescence microscope. ps cells infected with vsv contrasted to l cells infected with the same virus in the following two points; (1) the principal s ... | 1977 | 205763 |
| (s)-9-(2,3-dihydroxypropyl)adenine: an aliphatic nucleoside analog with broad-spectrum antiviral activity. | (s)-9-(2,3-dihydroxypropyl)adenine, a novel nucleoside analog, the sugar moiety of which is replaced by an aliphatic chain, inhibits the replication in vitro of several dna and rna viruses, including vaccinia, herpes simplex (types 1 and 2), measles, and vesicular stomatitis. it is also effective in vivo in reducing the mortality rate of mice inoculated intranasally with vesicular stomatitis virus. | 1978 | 205946 |
| prolonged infection of l cells with vesicular stomatitis virus. defective interfering forms and temperature-sensitive mutants as factors in the infection. | | 1978 | 206006 |
| proteins of vesicular stomatitis virus. iv. a comparison of tryptic peptides of the vesicular stomatitis group of rhabdoviruses. | | 1978 | 210582 |
| isolation of possible replicative intermediate structures from vesicular stomatitis virus-infected cells. | | 1978 | 206007 |
| shope fibroma virus-induced facilitation of vesicular stomatitis virus adsorption and replication in nonpermissive cells. | | 1978 | 206013 |
| interaction of vesicular stomatitis virus with lipid vesicles: depletion of cholesterol and effect on virion membrane fluidity and infectivity. | interaction with excess unilamellar phosphatidylcholine (pc) vesicles resulted in depletion of as much as 90% of the cholesterol from the membrane of intact vesicular stomatitis (vs) virus. the cholesterol depletion was not significantly influenced by the proteolytic removal of virion glycoprotein spikes, but it was temperature dependent. cholesterol depletion caused substantial reduction in anisotropy of the vs virion membrane as measured by fluorescence depolarization of the lipophilic probe 1 ... | 1978 | 211263 |
| shedding of the glycoprotein from vesicular stomatitis virus-infected cells. | in a culture of chinese hamster ovary cells infected with vesicular stomatitis virus, there is specific shedding of viral antigens into the medium. this shedding appears to be unrelated to progeny formation or to cell lysis. although all five of the virus-specific proteins are detected in the extracellular soluble fraction, the major antigen is the gs protein. this protein has a molecular weight of 54,000. indirect analysis of the content of sialic acid as well as peptide analysis of the gs and ... | 1978 | 211264 |
| lipid organization of the membrane of vesicular stomatitis virus. | | 1978 | 207703 |
| in situ cross-linking of vesicular stomatitis virus proteins with reversible agents. | | 1978 | 211712 |
| inhibition of translation by poliovirus: inactivation of a specific initiation factor. | translation of vesicular stomatitis virus (vsv) mrna, like host mrna translation, is inhibited in cells infected with poliovirus. to study the mechanism of poliovirus-induced inhibition of protein synthesis, we prepared extracts from poliovirus-infected and uninfected hela cells. poliovirus mrna was translated in lysates from both infected and uninfected cells, while vsv mrna was translated only in the lysate from uninfected cells. addition of purified translation initiation factors to the extra ... | 1978 | 208073 |
| in vitro synthesis of a possible precursor rna by purified vesicular stomatitis virus. | | 1978 | 208572 |
| asymmetric distribution of phosphatidylethanolamine fatty acyl chains in the membrane of vesicular stomatitis virus. | the membrane of vesicular stomatitis virus (vsv) contains two distinct pools of phosphatidylethanolamine molecules which reside in the inner and outer phospholipid monolayers, respectively. 36% of the total membrane phosphatidylethanolamine is found in the outer monolayer while 64% is found in the inner. the two pools of vsv phosphatidylethanolamine can be distinguished operationally by the fact that only outer phosphatidylethanolamine is reactive in intact virions with the membrane-impermeable ... | 1978 | 208607 |
| isolation of the envelope of vesicular stomatitis virus. | vesicular stomatitis virus was disrupted by a combination of freezing and thawing, osmotic shock, and sonic treatment. subviral components were separated by isopycnic centrifugation. the low-density, lipid-rich fractions were pooled and shown to contain primarily viral glycoprotein. further purification of this material resulted in the isolation of a preparation of vesicles which contained only the g protein and the same phospholipids as in the intact virions and exhibited spikelike structures s ... | 1978 | 209217 |
| antibody-independent neutralization of vesicular stomatitis virus by human complement. i. complement requirements. | | 1978 | 212482 |
| assignment of the receptor for ecotropic murine leukemia virus to mouse chromosome 5. | the gene for the receptor for ecotropic murine leukemia virus (rev) has been assigned to mouse chromosome 5. this determination was made possible by an analysis of somatic cell hybrids between mouse and chinese hamster cells. the parents of these hybrids were a/hej or mus poschiavinus peritoneal exudate cells or balb/c primary embryo fibroblasts and e36, a chinese hamster lung fibroblast deficient in hypoxanthine guanine phosphoribosyltransferase. segregation of mouse chromosomes in these hybrid ... | 1978 | 212501 |
| [formation of pseudotypes of vsv after culture in a human melanoma]. | electron microscope studies showed a high production of melanosomes and viral particles budding into the cisternae of the endoplasmic reticulum in cells derived from a subcutaneous metastasis. the history of this subline (hm6b-a) has been summarized elsewhere. an amelanotic subline (provided by the same patient) did not show similar virus particles. when infected with a vesicular stomatitis virus (vsv) thermolabile mutant (tl), these cells produced a vsv pseudotype in which a thermosensitive ant ... | 1978 | 209712 |
| synthesis of as-triazines as potential antiviral agents. | four acenaphtho[1,2-e]-as-triazines and 11 5,6-diaryl-as-triazines, all substituted with an aliphatic or aromatic amino function in the 3-position, were synthesized. two acenaphthotriazines were active against vesicular stomatitis virus in tissue culture. | 1978 | 205646 |
| inhibition of rna synthesis in mouse myeloma cells infected with vesicular stomatitis virus. | infection of mouse myeloma cells (mpc-11) with vesicular stomatitis (vs) virus resulted in rapid and marked reduction in cellular rna synthesis considerably before cell viability was compromised. mouse myeloma cells responded maximally to viral infection at a multiplicity of 1 and were considerably more se;sitive to shut-off of rna synthesis than were mouse l cells or bhk-21 cells. this inhibition of cellular rna synthesis was shown not to be caused by differential membrane permeability of infec ... | 1978 | 205671 |
| target antigens for h-2-restricted vesicular stomatitis virus-specific cytotoxic t cells. | cytotoxic thymus-derived lymphocytes from mice infected with vesicular stomatitis virus (vsv) are h-2 restricted and virus specific for the indiana and new jersey strains of vsv. vsv-indiana-immune t cells can lyse target cells infected with the temperature sensitive (ts) mutant ts 045 about 30 times better when target cell infection occurs at the permissive rather than the non-permissive temperature. since this mutant fails to express the glycoprotein at the cell surface when grown at the nonpe ... | 1978 | 210232 |
| assembly of viral membranes: maturation of the vesicular stomatitis virus glycoprotein in the presence of tunicamycin. | the role of glycosylation in the maturation of the vesicular stomatitis virus (vsv) glycoprotein was studied by use of the antibiotic tunicamycin. tunicamycin-treated vsv-infected cells synthesize an unglycosylated form of the vsv glycoprotein (r. leavitt, s. schlesinger, and s. kornfeld, j. virol. 21:375--385, 1977). we have found that tunicamycin has no effect on the attachment of the glycoprotein to intracellular membranes or on the transport of protein to the lumen of the endoplasmic reticul ... | 1978 | 212608 |
| nucleotide sequence homology at the 3' termini of rna from vesicular stomatitis virus and its defective interfering particles. | vesicular stomatitis virus (vsv) and defective interfering (di) particle rnas were labeled at their 3' ends by using rna ligase and cytidine 3',5'-bis[32p]phosphate. the rnas were subjected to partial digestion with alkali and analyzed by oligonucleotide fingerprinting in two dimensions. vsv and di particle rnas have complete sequence homology for the first eight bases from the 3' end. the following four positions contain three mismatched nucleotides in which guanosine residues in one strand are ... | 1978 | 210454 |
| persistent infection. i interferon-inducing defective-interfering particles as mediators of cell sparing: possible role in persistent infection by vesicular stomatitis virus. | | 1978 | 206002 |
| antiviral activity in interferon-treated bovine tracheal organ cultures. | the effect of bovine interferon on the replication of infectious bovine rhinotracheitis virus and vesicular stomatitis virus in bovine tracheal organ cultures was studied. after treatment of tracheal organ cultures with interferon, inhibition of infectious bovine rhinotracheitis virus and vesicular stomatitis virus replication was observed. this tracheal organ system may be useful in determining the in vivo response to interferon for viral infections of the bovine respiratory tract. | 1978 | 211089 |
| replication of herpesviruses in human cells transformed by cytomegalovirus. | the replication of human cytomegalovirus (cmv) and herpes simplex virus (hsv) was studied in three human embryo cell lines (cmv-mj-hel-i, cmv-mj-hel-2, and cmv-mj-hel-2,t-i) transformed in vitro by human cmv. growth studies revealed that these cells were completely resistant to infection by cmv strains adi69 and mj and partially resistant to hsv types i and 2. neither virus dna nor virus proteins were synthesized in the transformed cells infected with cmv ad169. the hsv production in cmv-transfo ... | 1978 | 211187 |
| membrane assembly: synthesis and intracellular processing of the vesicular stomatitis viral glycoprotein. | the glycoprotein (g) of vesicular stomatitis virus (vsv) is synthesized on membrane-bound polyribosomes. approximately 30 min after its synthesis, it reaches the surface plasma membrane where it is incorporated into budding virus. the first part of this paper focuses on the 2 intracellular, membrane-bound, glycosylated forms of the glycoprotein which are intermediates in its biogenesis. all glycosylation and processing is completed in the smooth microsome fraction before the protein reaches the ... | 1977 | 211348 |
| inhibition of vesicular stomatitis virus replication by frog virus 3. selective action on secondary transcription. | | 1978 | 213882 |
| length of glycoprotein spikes of vesicular stomatitis virus and sindbis virus, measured in situ using quasi elastic light scattering and a resistive-pulse technique. | | 1978 | 213892 |
| antiviral effects of amphotericin b methyl ester. | the methyl ester of amphotericin b (ame) is water soluble, retains antifungal activity, and is significantly less toxic in mammals than amphotericin b. in contrast to amphotericin b, which is not water soluble, ame exhibits antiviral effects against vesicular stomatitis virus, herpes simplex virus types 1 and 2, sindbis virus, and vaccinia virus in a plaque reduction assay. no antiviral effects could be demonstrated against the unenveloped adenovirus type 4 or echovirus type 11. the extent of vi ... | 1978 | 206201 |
| effect of amphotericin b methyl ester on vesicular stomatitis virus morphology. | the water-soluble methyl ester of amphotericin b inactivates vesicular stomatitis virus in association with morphological alterations of the envelope. | 1978 | 206202 |
| assembly of viral membranes: nature of the association of vesicular stomatitis virus proteins to membranes. | to explore the interaction of vesicular stomatitis virus (vsv) proteins with cellular membranes, we have isolated membranes from infected cells that have been radioactively pulse-labeled. we have found conditions of isolation that result in membrane preparation which contain primarily the vsv membrane protein (m) and glycoprotein (g). both of these proteins are very firmly attached to membranes: conditions known to release peripherally associated membrane proteins from membranes (s. razin, bioch ... | 1978 | 206719 |
| in vitro rna transcription by the new jersey serotype of vesicular stomatitis virus. i. characterization of the mrna species. | the in vitro rna synthesis by the virion-associated rna polymerase of vesicular stomatitis virus (vsv), new jersey serotype, was compared with that of the serologically distinct indiana serotype of vsv. the new jersey serotype of vsv synthesized five distinct mrna species in vitro, three of which were smaller than the corresponding species synthesized by the indiana serotype of vsv. these included the mrna's coding for the g, m, and ns proteins. by hybridization experiments, virtually no sequenc ... | 1978 | 206724 |
| in vitro rna transcription by the new jersey serotype of vesicular stomatitis virus. ii. characterization of the leader rna. | the new jersey serotype of vesicular stomatitis virus (vsv) was able to synthesize a small rna (leader rna) approximately 70 bases in length similar to the leader rna synthesized in vitro by the genetically distinct indiana serotype of vsv. also, the new jersey leader rna contained the same 5'-terminal sequence, ppa-c-g, as the indiana leader rna and had a very similar base composition, with 42% amp, 16% cmp, 18.6% gmp, and 23.4% ump. the 3'-terminal sequence of the vsv new jersey genome rna was ... | 1978 | 206725 |
| efficient transfer of interferon-induced virus resistance between human cells. | the rate of development of interferon-induced virus resistance in a mixture of two human cell types (u and wish) is determined by the cell type (wish) in the mixture which responds first. this phenomenon has been shown with two types of interferon assay procedure, and with both vesicular stomatitis virus and sindbis virus. the transfer of virus resistance from one human cell (wish) to another (u) (homospecific transfer) is much more efficient than the transfer from mouse l cells to wish cells (h ... | 1978 | 214520 |
| effect of interferon on transcription and translation of vesicular stomatitis virus in human and simian cell cultures. | the effect of interferons on vesicular stomatitis virus (vsv) primary transcription, amplified rna synthesis [i.e. the sum of primary transcription rna replication (leading to [ - ] rna) and secondary transcription (leading to [ + ] rna) and virus protein synthesis were studied. in a human cell line, both human and simian interferons inhibited the initiation of primary transcription and amplified rna synthesis. in contrast, in a simian cell line tested similarly, the initiation of these activiti ... | 1978 | 214523 |
| synthesis and infectivity of vesicular stomatitis virus containing nonglycosylated g protein. | the replication of vesicular stomatitis virus (vsv) is inhibited by tunicamycin (tm), an antibiotic that blocks the formation of n-acetylglucosaminelipid intermediates. we had shown previously that the viral glycoprotein (g) synthesized in cells treated with tm is not glycosylated and is not found on the outer surface of the cell plasma membrane. in this report, we shown that cells exposed to tm produce a low yield of infectious particles. the yield is increased when the temperature during infec ... | 1978 | 207437 |
| in vitro functional analysis of a temperature-sensitive mutant of vesicular stomatitis virus, new jersey serotype, defective in transcription. | | 1978 | 214958 |
| the entry into host cells of sindbis virus, vesicular stomatitis virus and sendai virus. | we have compared the mechanisms of entry into host cells of three enveloped viruses: sendai virus, vesicular stomatitis virus (vsv) and sindbis virus. virus entry by membrane fusion should antigenically modify the surface of a newly infected cell in such a way that it will be killed by anti-viral antibody and complement. on the other hand, virus entry by a mechanism involving uptake by the cell of the whole virion should not make cells sensitive to antibody and complement. as expected, cells new ... | 1978 | 215317 |
| evidence against the role of k+ in the shut-off of protein synthesis by vesicular stomatitis virus. | intracellular k+ ion concentration and transport were measured in l cells infected with wild type vsv, which rapidly inhibits total protein synthesis in infected cells, and with a mutant, ri, defective in this function. no alterations in intracellular k+ ion concentration or transport were observed until late in infection and the late changes seen were similar for both viruses. thus the inhibition of total protein synthesis by vsv cannot be ascribed to virus induced changes in host k+ ion concen ... | 1978 | 207821 |
| host range restriction of vesicular stomatitis virus on duck embryo cells. | | 1978 | 208241 |
| aggregation and thermolability of some group v (g protein) and group iii (m protein) mutants of vesicular stomatitis virus. | | 1978 | 208269 |
| rescue at nonpermissive temperature of complementation group ii temperature-sensitive mutants of vesicular stomatitis virus by uv-irradiated vsv. | | 1978 | 208272 |
| factors which influence inactivation of vesicular stomatitis virus by fresh human serum. | | 1978 | 208281 |
| intracellular events in the replication of defective interfering particles of vesicular stomatitis virus. | | 1979 | 217161 |
| complete exchange of viral cholesterol. | the exchange of the cholesterol in the membranes of two enveloped viruses, sindbis virus and vesicular stomatitis virus, with cholesterol present in lipid vesicles and in serum was measured. biosynthetically labeled viral cholesterol underwent spontaneous and complete transfer to both lipid vesicles and to serum. the rate with which and the extent to which this process occurred were very similar for these two viruses. during incubation with lipid vesicles in excess, half of the viral cholesterol ... | 1979 | 217416 |
| compositional asymmetry and transmembrane movement of phosphatidylcholine in vesicular stomatitis virus membranes. | | 1979 | 217417 |
| cellular interactions determine the rate and degree of interferon action. | the rate and degree of interferon action on mouse embryo (me), mouse l, and human amnion (wish) cells were found to be dependent on the cell density. the most precipitous drop in interferon action occurred just below cell confluency. this effect was shown with both vesicular stomatitis virus and sindbis virus and at both constant and variable input multiplicities of infection. at both "high" and "low" cell densities, cells attached to a surface develop viral resistance faster than suspended cell ... | 1979 | 217833 |
| the synthesis of complex-type oligosaccharides. i. structure of the lipid-linked oligosaccharide precursor of the complex-type oligosaccharides of the vesicular stomatitis virus g protein. | the synthesis of the complex-type oligosaccharide unit of the vesicular stomatitis virus g protein is initiated by the en bloc transfer of a high molecular weight oligosaccharide from a lipid carrier to the nascent polypeptide. following transfer the oligosaccharide is "processed" by removal of glucose and mannose residues and the sugars that constitute the outer branches of the complex-type oligosaccharide are added. the structure of the oligosaccharide moiety of the lipid-linked precursor has ... | 1978 | 212434 |
| the synthesis of complex-type oligosaccharides. ii. characterization of the processing intermediates in the synthesis of the complex oligosaccharide units of the vesicular stomatitis virus g protein. | | 1978 | 212435 |
| virus-replicating t cells in the immune response of mice. ii. characterization of t cells capable of replicating vesicular stomatitis virus. | immunocytological properties of the splenic t cell (tv) which develop into virus plaque-forming cells in response to the antigenic challenge in vitro were investigated in relation to the properties of helper t cells and suppressor t cells in antibody response. tv was observed in spleen around 1 wk after the intravenous injection of mice with 10(7) sheep erythrocytes. this contrasted with the finding that both helper t cells and suppressor t cells developed as early as 3 days after the immunizati ... | 1978 | 212507 |
| absence of interferon production in a newly established human cell line. | a cell line established from human embryonic lung, hel-r66, was demonstrated to be highly susceptible to herpes simplex virus types 1 and 2, vaccinia virus, newcastle disease virus (ndv), japanese encephalitis virus (jev), western equine encephalitis (wee) virus, sindbis virus, vesicular stomatitis virus (vsv), and rabies virus. the maximal yields of ndv, jev, wee virus, and rabies virus in this cell line exceeded by 2--4 logs those in control human embryonic lung cells. inability of this cell l ... | 1979 | 218901 |
| biosynthesis of lipid-linked oligosaccharides. isolation and structure of a second lipid-linked oligosaccharide in chinese hamster ovary cells. | previous work has shown that vesicular stomatitis virus-infected chinese hamster ovary cells contain a major high molecular weight lipid-linked oligosaccharide which is transferred en bloc to protein during the formation of the asparagine-linked complex-type oligosaccharides of the vesicular stomatitis virus g protein (tabas, i., schlesinger, s., and kornfeld, s. (1978) j. biol. chem. 253, 716-722). we now report the characterization of a second, lower molecular weight lipid-linked oligosacchari ... | 1979 | 218953 |
| virus-replicating t cells in the immune response of mice. iii. role of vesicular stomatitis virus-replicating t cells in the antibody response. | the functional role of the t cell (tv) which can replicate vesicular stomatitis virus (vsv) on activation by the antigen was investigated in antibody response in vitro. by the inoculation of vsv into the culture, marked augmentation of antibody response to sheep erythrocytes (srbc) was observed in the culture of spleen cells taken more than 3 days after the immunization with srbc, suggesting that the vsv-susceptible suppressor cells were included in these spleen cells and the activity was elimin ... | 1978 | 212508 |
| rebinding of transcriptase components (l and ns proteins) to the nucleocapsid template of vesicular stomatitis virus. | the l and ns proteins of vesicular stomatitis virions (new jersey serotype) were solubilized with triton x-100 and high-salt buffer and recombined with purified nucleocapsids under conditions similar to those used to reconstitute transcriptase activity in vitro. the nucleocapsid-bound l and ns proteins were separated from unbound proteins on a glycerol gradient. the rebinding of l and ns proteins mimics the in vivo binding in that at saturation the ratio of l and ns molecules to n molecules is a ... | 1978 | 212581 |
| specificity of interferon action in protein synthesis. | inhibitors of elongation steps in protein synthesis such as cycloheximide and anisomycin mimic interferon treatment in that they specifically inhibit the synthesis of certain viral proteins. these specific effects are seen only at very low concentrations of the antibiotics, under conditions where host cellular protein synthesis, as well as cell viability, are not severely reduced. a qualitatively as well as quantitatively close correlation between the effects of the two types of agents has been ... | 1978 | 212587 |
| persistent infection of l cells with vesicular stomatitis virus: evolution of virus populations. | a previous report (youngner et al., j. virol. 19:90-101, 1976) documented that noncytocidal persistent infection can be established with wild-type vesicular stomatitis virus (vsv) in mouse l cells at 37 degrees c and that a rapid selection of rna(-), group i temperature-sensitive (ts) mutants consistently occurs in this system. to assess the selective advantage of the rna(-)ts phenotype, evolution of the virus population was studied in persistent infections initiated in l cells by use of vsv ts ... | 1978 | 212614 |
| transmembrane movement and distribution of cholesterol in the membrane of vesicular stomatitis virus. | the transmembrane movement and distribution of cholesterol in the vesicular stomatitis virus membrane were studied by following the depletion of cholesterol from virions to interacting phospholipid vesicles and by exchange of radiolabeled cholesterol between virions and phospholipid-cholesterol vesicles. the kinetics of the cholesterol exchange or depletion reactions revealed the presence of two exponential rates: a rapid rate, dependent on the vesicle to virus ratio, and a slower rate, independ ... | 1978 | 213106 |
| lipids with photosensitive groups as chemical probes for the structural analysis of biological membranes. on the localization of the g- and m-protein of vesicular stomatitis virus. | | 1978 | 213365 |
| resistance of visna virus to interferon. | visna is a slow infection of sheep caused by a retrovirus. the persistence of virus despite the immune response of the host is best explained by restricted genetic expression of the virus and consequently prolonged periods of residence inside cells. the purpose of this investigation was to determine whether the restriction in genetic expression of visna virus is mediated by interferon. sheep interferon induced by polyriboinosinic-polyribocytidylic acid in fetal lambs inhibited the growth of herp ... | 1978 | 213502 |
| temperature-sensitive mutant ts o82 of vesicular stomatitis virus. i. rescue at nonpermissive temperature by uv-irradiated virus. | | 1979 | 219603 |
| a comparative study of the surface projections of different strains of vesicular stomatitis virus. | | 1975 | 213529 |
| coinfection with a rhabdovirus: vesicular stomatitis virus of indiana and new-jersey serotypes. | coinfection of cells with vesicular stomatitis virus (vsv) of indiana and new-jersey serotypes were performed. thermosensitive mutants (ts) of vsv indiana and the wild type strain (+) of new-jersey were used. harvests and titrations were made at permissive(pt) and nonpermissive (npt) temperatures. it was shown that the harvest was mainly composed of one parental-like infectious particles. the dominance of one serotype over the other was shown to be a function of the relative multiplicity of the ... | 1978 | 214003 |
| antiviral activity of intranasally applied human leukocyte interferon. | previous studies in our laboratory have demonstrated that the development of antiviral activity of human leukocyte interferon (if) in nasal epithelial cells is time and concentration dependent and that the loss of intranasally applied human leukocyte if is rapid. the present studies compared the activity of if applied intranasally either by nasal drops or by a saturated cotton pledget. adult volunteers had if applied to an area of nasal mucosa (2 by 2 cm(2)) either by repeated nose drops or by a ... | 1978 | 214028 |
| intervening polyadenylate sequences in rna transcripts of vesicular stomatitis virus. | purified and partially resolved vesicular stomatitis virus (vsv) messenger rna has been annealed to the vsv genomic rna and visualized in the electron microscope under conditions in which duplex regions have a wider image width than single-stranded rna. the locations of the intercistronic boundaries between the messages have been mapped on the vsv genome. the contour of the double-stranded regions is occasionally interrupted by looped-out single-stranded rna. the loops are comprised of post tran ... | 1978 | 214245 |
| the tdce and hrce phenotypes: host range mutants of vesicular stomatitis virus in which polymerase function is affected. | | 1978 | 214246 |
| a signal sequence for the insertion of a transmembrane glycoprotein. similarities to the signals of secretory proteins in primary structure and function. | the biosynthesis of a secretory protein and a transmembrane viral glycoprotein are compared by two different experimental approaches. (a) nh2-terminal sequence analysis has been performed on various forms of the transmembrane glycoprotein of vesicular stomatitis virus synthesized in cell-free systems. the sequence data presented demonstrate that the nascent precursor of the glycoprotein contains a "signal sequence" of 16 amino acids at the nh2 terminus, whose sequence is met-lys-cys-leu-leu-tyr- ... | 1978 | 214427 |
| susceptibility and serological response of cattle, pigs, horses and goats to indiana2 and indiana3 strains of vesicular stomatitis virus. | | 1977 | 220637 |
| nucleotide sequence of the leader rna of the new jersey serotype of vesicular stomatitis virus. | sequence for the leader rna synthesized by the new jersey serotype of vesicular stomatitis virus is presented and its complementary sequence representing the 3'-terminal sequence of the genome rna is deduced. comparison with the leader rna sequence of the serologically distinct indiana strain reveals that the 3'-terminal region of the genomes of two viruses is highly conserved. | 1978 | 214766 |
| the matrix (m) protein of vesicular stomatitis virus regulates transcription. | temperature-sensitive mutants of vesicular stomatitis virus (vsv) belonging to complementation group iii contain a lesion in the matrix (m) protein. this results in a 2--5 fold increase in transcription at the nonpermissive temperature. co-infection of cells with one of these mutants and wild-type virus reverses this mutant phenotype. separation of the transcriptional and translational products from mutant-infected cells reveals an overall increase in each of the viral mrna species concomitant w ... | 1978 | 215330 |
| the complete sequence of a unique rna species synthesized by a di particle of vsv. | the 2s rna synthesized in vitro by the rna polymerase of a defective interfering (di) particle of vesicular stomatitis virus was labeled at its 3' terminus with 32p-cytidine 3', 5' bisphosphate and rna ligase. analysis of the labeled rna showed that it was a family of rnas of different length but all sharing the same 5' terminal sequence. the largest labeled rna was purified by gel electrophoresis, and the sequence of 41 of its 46 nucleotides was determined by rapid rna sequencing methods. the a ... | 1978 | 212197 |
| pseudotypes of vesicular stomatitis virus and pichinde virus. | super-infection of pichinde virus-infected cells with vesicular stomatitis virus (vsv) resulted in the production of pseudotype virus which was not neutralized by antiserum to vsv but which was neutralized by antiserum to pichinde virus. analysis of pseudotype virus production in relation to the kinetics of replication of pichinde virus demonstrated that pseudotype virus production occurred when super-infection with vsv was initiated 8 h or more after infecting the cells with pichinde virus. the ... | 1979 | 215705 |
| complete nucleotide sequence of the leader rna synthesized in vitro by vesicular stomatitis virus. | the complete nucleotide sequence of the leader rna synthesized in vitro by the indiana serotype of vesicular stomatitis virus is presented. the sequence was determined by the technique described by donis-keller, maxam and gilbert (1977) in combination with the standard two-dimensional fingerprint techniques described by barrell (1971). the leader rna contains 48 nucleotides variably terminating at the 3' terminus with cytosine (68%) and adenosine at position 47 (32%). since the leader rna is com ... | 1978 | 212201 |
| gycoprotein and protein precursors to plasma membranes in vesicular stomatitis virus infected hela cells. | vesicular stomatitis virus is known to mature at hela cell plasma membranes. to study the process, cells, infected with vesicular stomatitis virus, were fractionated after short term labeling studies (1 min pulse, 1 min chase) to determine the assembly kinetics of g protein and m protein into plasma membranes. newly synthesized m protein was found released in the supernatant from which free polysomes were sedimented during sucrose gradient analysis of these polysomes. if this m protein is partic ... | 1978 | 215836 |
| translation of capped and uncapped vesicular stomatitis virus and reovirus mrna's. sensitivity to m7gpppam and ionic conditions. | in an attempt to elucidate the role of the 5'-terminal 7-methylguanosine residue in translation of mammalian mrnas, vesicular stomatitis virus (vs virus), and reovirus mrnas containing and lacking this residue, and also qbeta rna, were translated in cell-free extracts from reticulocytes and wheat germ under a variety of ionic conditions. optimal translation of mrnas lacking a 5' m7g occurred at concentrations of koac or kcl which were lower than those optimal for normal "capped" mrnas. however, ... | 1979 | 216675 |
| sequence of a rna templated by the 3'-oh rna terminus of defective interfering particles of vesicular stomatitis virus. | we have sequenced the endogenous rna polymerase product produced by disrupted purified virions of vesicular stomatitis virus defective interfering particles by using the newer one-dimensional rapid gel sequencing techniques and confirming this with a modified two-dimensional gel vectoring technique. the sequence of this 46-nucleotide rna is: 5'(pp)pacgaagaccacaaaacca-gauaaaaaauaaaaaccacaagaggg(u)coh3'. we infer that this sequence is identical to the sequence at the 5' end of infectious vesicular ... | 1978 | 216996 |
| neutralization of mason-pfizer virus by sera from patients treated for renal disease. | sera from 67 patients treated for renal diseases were assayed by as many as three different tests for activities against mason-pfizer virus (m-p v) antigens. firstly, in patients studied before kidney transplantation, neutralizing activity against syncytium-forming units of m-p v was found in 50% of 24 cases of chronic glomerulonephritis but in only 10% of 20 cases with other diseases (p less than 0.01). these proportions were higher after treatments accompanying transplantation since, of the 19 ... | 1978 | 217951 |
| synthesis and assembly of membrane glycoproteins: presence of leader peptide in nonglycosylated precursor of membrane glycoprotein of vesicular stomatitis virus. | translation of mrna encoding vesicular stomatitis virus envelope glycoprotein g by as membrane-free ribosomal extract obtained from hela cells yielded a nonglycosylated protein (g1 (mr 63,000). in the presence of added microsomal membranes, g1 was converted to the glycosylated protein (g2 (mr 67,000) which is inserted in the membrane vesicles as a transmembrane protein. labeling with methionine donated by wheat germ initiator trna1met showed that g1 but not g2 contains methionine in the nh2-term ... | 1979 | 218209 |
| the nonglycosylated glycoprotein of vesicular stomatitis virus is temperature-sensitive and undergoes intracellular aggregation at elevated temperatures. | | 1979 | 218976 |
| envelope proteins and replication of vesicular stomatitis virus: in vivo effects of rna+ temperature-sensitive mutations on viral rna synthesis. | temperature-sensitive (ts) mutants of vesicular stomatitis virus belonging to complementation groups iii and v were investigated for their in vivo rna synthesis. the sucrose gradient patterns of the rna species which they produced at nonpermissive temperature (39.2 degrees c) were systematically compared under different experimental conditions: variation of input multiplicity and of time of infection, superinfection with t particles, and temperature shifts. finally, a more precise analysis of th ... | 1979 | 219212 |
| decay of vesicular stomatitis virus mrnas in vivo. | | 1979 | 222066 |
| initiation of rna synthesis in vitro by vesicular stomatitis virus. role of atp. | the atp requirement during rna transcription by the rna-dependent rna polymerase associated with purified vesicular stomatitis virus was studied during chain initiation and chain elongation steps. initiation of transcription in vitro has an apparent km for atp of approximately 500 micron, whereas the apparent km for atp during chain elongation is almost identical with those for the other three ribonucleoside triphosphates, i.e. 20 to 30 micron. further analysis of the transcription process demon ... | 1979 | 217877 |
| role of the membrane (m) protein in endogenous inhibition of in vitro transcription by vesicular stomatitis virus. | an endogenous transcriptase inhibitor active at high concentrations of vesicular stomatitis (vs) virus was present in trypsinized whole virions but was absent from ribonucleoprotein cores containing only the l, n, and ns proteins. poly(l-glutamic acid) effectively reversed the transcriptase inhibition. transcription under noninhibited, inhibited, and poly(l-glutamic acid)-reversed conditions did not appear to greatly affect the nature of the rna transcription product. the vs virion matrix (m) pr ... | 1979 | 219213 |
| morphological and biochemical characterization of viral particles produced by the tso45 mutant of vesicular stomatitis virus at restrictive temperature. | the growth at restrictive temperature of tso45, a group v (glycoprotein) conditional lethal mutant of vesicular stomatitis virus (vsv), was demonstrated to result in the production of large numbers of noninfectious viral particles. the infectivity of these tso45 particles could be enhanced by procedures known to promote membrane fusion. morphologically and biochemically these particles differed from wild-type vsv by their lack of viral glycoprotein. the other structural proteins of vsv were pres ... | 1979 | 219217 |
| specific protein phosphorylation in interferon-treated uninfected and virus-infected mouse l929 cells: enhancement by double-stranded rna. | the enhanced phosphorylation of specific protein(s) observed in extracts from interferon-treated cells (in the presence of atp and double-stranded [ds] rna) was also seen in intact mouse l929 cells upon treatment with dsrna, polyriboinosinic.polyribocytidylic acid [poly(ri.rc)] or reovirus dsrna, using 32pi as radiolabel. labeling of a 65,000-dalton protein(s) with 32p was greatly increased in interferon-treated cells in the presence of added dsrna, suggesting that the expression in vivo of the ... | 1979 | 219224 |
| growth and maturation of a vesicular stomatitis virus temperature-sensitive mutant and its central nervous system isolate. | a temperature-sensitive (ts) mutant of vesicular stomatitis virus (vsv), tsg31, produces a prolonged central nervous system disease in mice with pathological features similar to those of slow viral diseases. tsg31 and the subsequent virus recovered from the central nervous system (tsg31bp) of mice infected with tsg31 were compared with the parental wild-type (wt) vsv for plaque morphology, growth kinetics, thermal sensitivity of the virions, and viral protein synthesis and maturation. several pr ... | 1979 | 219225 |
| noninfectious vesicular stomatitis virus particles deficient in the viral nucleocapsid. | several temperature-sensitive mutants of vesicular stomatitis virus in complementation group iii produce, at nonpermissive temperature, noninfectious particles which contain the viral m (matrix) and g (glycoprotein) proteins but less than 10% of the normal proportion of n protein or rna. since group iii mutants are thought to be defective in the structural gene for the virus m protein, these findings demonstrate that an interaction between m and the nucleocapsid is of importance in virus budding ... | 1979 | 219239 |
| [effect of host cells on the course of chronic rabies virus infection in cell cultures]. | different patterns of rabies virus infection were observed in bhk-21/13s and hep-2 cell cultures at late stages of persistent infections. the infection of bhk-21/13s cells was characterized by periodical increases and declines in the portion of the antigen-containing cells (from 100% to less than 1%) and low infectivity titers which did not correspond to fluctuations in the antigen production and occasional resistance to challenge with vesicular stomatitis virus. in contrast, persistently infect ... | 1978 | 219626 |
| heterospecific activity of rat interferon. | in the experiments performed in vitro and in vivo it has been found that the rat and rat embryo fibroblasts cultured in vitro after the induction with virus produce interferon which displays the antiviral activity not only in the homologous cells but also in the heterologous ones. when analysed by chromatography on sephadex g-100 it was shown that the rat serum contains two interferon populations differing in the molecular weight and both active in the homologous and heterologous cells. the inte ... | 1978 | 219806 |
| neutralization of pseudotypes of vesicular stomatitis virus by sera from avian retrovirus-infected hosts. | we investigated the capacity of lymphocytes and sera from chickens bearing tumors induced by avian sarcoma viruses (asv) to interact with phenotypically mixed particles of vesicular stomatitis virus (vsv) and asv. immune chicken sera were able to specifically neutralize such vsv pseudotypes. this ability could be absorbed out, however, on purified preparations of avian retroviruses, suggesting that reactivity was primarily against avian retrovirus enveloped components. supernatant fluids contain ... | 1979 | 220198 |
| reconstitution into liposomes of the glycoprotein of vesicular stomatitis virus by detergent dialysis. | the glycoprotein of vesicular stomatitis (vs) virus was selectively liberated from the virion membrane by the dialyzable nonionic detergent, beta-d-octylglucoside. the isolated viral glycoprotein could be rendered virtually free of phospholipid and detergent, under which conditions it formed tail-to-tail glycoprotein micelles in the form of rosettes. when mixtures of viral glycoprotein and egg lecithin were dialyzed free of octylglucoside, glycoprotein vesicles formed spontaneously with spikes p ... | 1979 | 220238 |
| subacute infection with temperature-sensitive vesicular stomatitis virus mutant g41 in the central nervous system of mice. i. clinical and virologic studies. | inoculation of three- to four-week-old balb/c mice with temperature-sensitive (ts) vesicular stomatitis virus mutant g41 produced a subacute neurological disease, initially characterized by development of lethargy, hunched posture, and ruffled fur within five to seven days after infection. more than 90% of infected mice developed these clinical signs. in approximately 60% of infected mice, the initial neurological signs proceeded to striking hind-limb paralysis and weight loss. these signs usual ... | 1979 | 220328 |
| subacute infection with temperature-sensitive vesicular stomatitis virus mutant g41 in the central nervous system of mice. ii. immunofluorescent, morphologic, and immunologic studies. | inoculation of three- to four-week-old balb/c mice with temperature-sensitive (ts) vesicular stomatitis virus (vsv) mutant g41 produced a subacute neurological disease mainly localized in the spinal cord. meningitis and diffuse microglial infiltration of the anterior horns of the spinal cord were seen starting six days after infection when neuronal degenerative changes could be seen. infection of neurons was demonstrated by immunofluorescence microscopy five days after infection. two to three we ... | 1979 | 220329 |
| the inhibition of vesicular stomatitis virus protein synthesis by frog virus 3. | | 1979 | 220794 |