Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| an outbreak of abortion caused by the equine arteritis virus. | 1957 | 13397180 | |
| a virus-induced epizootic hemorrhagic disease of the virginia white-tailed deer (odocoileus virginianus). | a circumscribed natural outbreak of a highly fatal disease of deer, which we have designated epizootic hemorrhagic disease (ehd), has been studied. the disease has proven readily transmissible in deer but not in other experimental or domestic animals tested, nor in embryonating eggs or deer kidney cell cultures. the causative agent is a virus which is readily filterable and is capable of storage, either frozen or in glycerol, for relatively long periods of time. it produces a solid immunity in t ... | 1960 | 19867168 |
| composition of rna and dna of citric acid-isolated liver nuclei from hamsters infected with equine abortion virus (eav). | 1960 | 13827336 | |
| propagation of equine arteritis virus previously adapted to cell cultures of equine kidney in monolayer cultures of hamster kidney. | 1962 | 14007366 | |
| degradation of deoxyribonucleic acid and alteration nucleic acid metabolism in suspension cultures of l-m cells infected with equine abortion virus. | randall, charles c. (university of mississippi school of medicine, jackson) and barbara m. walker. degradation of deoxyribonucleic acid and alteration of nucleic acid metabolism in suspension cultures of l-m cells infected with equine abortion virus. j. bacteriol. 86:138-146. 1963.-metabolic alterations in log-phase suspension cultures infected with equine abortion virus (eav) were determined in l-m cells simultaneously labeled or prelabeled with h(3)- or c(14)-thymidine. although infection prod ... | 1963 | 14051805 |
| replication of a deoxyribonucleic acid virus in thymine-deficient mammalian cells. | gentry, glenn a. (university of mississippi school of medicine, jackson), lucy a. lawson, and charles c. randall. replication of a deoxyribonucleic acid virus in thymine-deficient mammalian cells. j. bacteriol. 88:1324-1328. 1964.-equine abortion virus (eav), a deoxyribonucleic acid (dna) virus, causes the degradation of host cell dna to acid-soluble components in the l-m cell. it was hypothesized that inhibitors of dna synthesis such as 5-fluorodeoxyuridine (fudr) and amethopterin, which act by ... | 1964 | 14234788 |
| [properties of the equine arteritis virus]. | 1965 | 4956350 | |
| biological and morphological aspects of the growth of equine abortion virus. | darlington, r. w. (st. jude children's research hospital, memphis, tenn.), and c. james. biological and morphological aspects of the growth of equine abortion virus. j. bacteriol. 92:250-257. 1966.-the growth of equine abortion virus (eav) was studied by bioassay and electron microscopy in l-cell monolayer and suspension cultures, and in hela and bhk 21/13 cell monolayers. results of virus assay (plaque-forming units) indicated that production of cell-associated virus (cav) began at 6 to 9 hr af ... | 1966 | 5941279 |
| further properties of equine arteritis virus. | 1966 | 4293706 | |
| kinetics of viral deoxyribonucleic acid, protein, and infectious particle production and alterations in host macromolecular syntheses in equine abortion (herpes) virus-infected cells. | infection of exponential-phase suspension cultures of mouse fibroblast cells (l-m) with equine abortion virus (eav) resulted in inhibition of cell growth and marked alterations in host metabolic processes. the synthesis of deoxyribonucleic acid (dna) and ribonucleic acid was inhibited within 4 hr after infection and was suppressed by more than 90% by the time of maximal virus replication (14 to 18 hr). the overall rate of protein synthesis, however, was similar in uninfected and virus-producing ... | 1968 | 4302745 |
| mycoplasmal deoxyribonuclease activity in virus-infected l-cell cultures. | cell-free extracts of mycoplasma hominis and medium from 72-hr broth cultures had deoxyribonuclease activity like that of deoxyribonuclease i. mg(++) stimulated activity, and the ph optimum was between 8.0 and 9.0. double-stranded or heatdenatured deoxyribonucleic acid (dna) served as a substrate, and oligonucleotides were produced. cell-free extracts of l cells infected with m. hominis or m. hominis plus equine abortion virus (equine herpes virus, eav) had greatly increased activity over that o ... | 1969 | 5782042 |
| a plaque assay of equine arteritis virus in bhk-21 cells. | 1969 | 4988871 | |
| buoyant density studies on equine arteritis virus. | 1970 | 4989678 | |
| neutralization of equine arteritis virus: enhancing effect of guinea pig serum. | 1970 | 4993579 | |
| [equine arteritis virus: multiplication in bhk 21-cells buoyant density and electron microscopical demonstration]. | 1970 | 4194811 | |
| morphological studies on equine arteritis virus. | 1970 | 4195609 | |
| equine arteritis virus: ferritin-tagging and determination of ribonucleic acid core. | 1971 | 4109496 | |
| [complement dependent neutralization of equine arteritis virus. brief report]. | 1971 | 4996685 | |
| temporal distribution of equine arteritis virus in respiratory mucosa, tissues and body fluids of horses infected by inhalation. | 1971 | 4999643 | |
| evidence for a relationship between equine abortion (herpes) virus deoxyribonucleic acid synthesis and the s phase of the kb cell mitotic cycle. | autoradiographic analyses of deoxyribonucleic acid (dna) synthesis in randomly growing kb cell cultures infected with equine abortion virus (eav) suggested that viral dna synthesis was initiated only at times that coincided with the entry of noninfected control cells into the s phase of the cell cycle. synchronized cultures of kb cells were infected at different stages of the cell cycle, and rates of synthesis of cellular and viral dna were measured. when cells were infected at different times w ... | 1971 | 4254680 |
| failure to propagate equine arteritis virus in an aedine and an anopheline mosquito species. | 1972 | 4402402 | |
| equine abortion (herpes) virus: strain differences in susceptibility to inactivation by dithiothreitol. | the infectivity of equine abortion (herpes) virus (eav) was inactivated by treatment with reduced dithiothreitol (dtt). according to their susceptibility to dtt, the eav strains could be divided into three groups. the vaccine strain rac-h (419) proved to be more resistant to dtt than all of the other 14 strains tested. the hemagglutinin of eav was also inactivated by dtt; no strain differences were observed in this respect. | 1972 | 4339509 |
| [route of inoculation and aluminium hydroxide influences in the immunological response of horses vaccinated against equine influenza (author's transl)]. | 1973 | 4377407 | |
| the role of sensitizing antibody in the neutralization of equine arteritis virus by complement or anti-igg serum. | 1973 | 4197221 | |
| the fate of sensitized equine arteritis virus following neutralization by complement of anti-igg serum. | 1973 | 4197222 | |
| structural proteins of equine arteritis virus. | 1973 | 4633581 | |
| equine abortion (herpes) virus: evaluation of markers in a field vaccination trial. | twelve mares were vaccinated with attenuated equine abortion virus (eav) strain rac-h. two nonvaccinated mares served as controls. in at least three mares the vaccination appeared to coincide with a natural infection. this was indicated by characterization of the eav isolated from nasal secretions of six vaccinated mares, a nonvaccinated control, and also from the lung, spleen, and liver of a fetus aborted by a vaccinated mare. the relative sensitivity of the isolated eav to dithiothreitol was u ... | 1973 | 4796167 |
| the complement-requiring neutralization of equine arteritis virus by late antisera. | 1973 | 4630828 | |
| the mechanisms of neutralization of sensitized equine arteritis virus by complement components. | 1974 | 4474357 | |
| antigenic relationship between the surface antigens of avian and equine influenze viruses. | influenza virus equine 1 (a/equine/prague/56) has a hemagglutinin which is antigenically related to the hemagglutinin of fowl plague virus strain rostock (fpv) and a neuraminidase which cross-reacts with the enzyme of virus n (a/chick/germany/49). after a single injection of chickens with equine 1 virus no hemagglutination inhibiting (hi) and neutralizing antibodies against fpv can be demonstrated, although the birds are fully protected against a lethal dose of fpv. hi and neutralizing antibodie ... | 1975 | 53781 |
| [new immunization schedule against equine influenza]. | 1975 | 166531 | |
| efficacy of 9-beta-d-arabinofuranosylhypoxanthine 5'-monophosphate in therapy of equine abortion virus-induced hepatitis in hamsters. | equine abortion virus (eav)-induced hepatitis in hamsters presents an interesting animal model for the evaluation of drugs possessing anti-deoxyribonucleic acid virus activity. these experiments demonstrate that 9-beta-d-arabinofuranosylhypoxanthine 5'-monophosphate (ara-hxmp), a new synthetic, water-soluble, antiviral agent, effectively controls this disease in hamsters with a therapeutic index of approximately 60. ara-hxmp prevented hepatitis-associated deaths in hamsters, reduced the titer of ... | 1975 | 172009 |
| studies on the multiplication of the equine arteritis virus using the fluorescent antibody technique. | 1975 | 172683 | |
| studies on equine viral arteritis. i. characterization of the virus and trial survey on antibody with vero cell cultures. | 1975 | 172689 | |
| the genome of equine arteritis virus. | 1975 | 173077 | |
| activation of influenza a viruses by trypsin treatment. | 1975 | 173078 | |
| immunofluorescent studies on the multiplication of equine arteritis virus in vero and e. derm (nbl-6) cells. | 1975 | 176497 | |
| studies on two viral strains isolated from the outbreak of equine influenza in japan. | 1975 | 183035 | |
| studies on a test vaccine for equine influenza virus. i. production of a test vaccine. | 1975 | 183036 | |
| studies on a test vaccine for equine influenza virus. ii. field trials. | 1975 | 183037 | |
| the structural proteins of equine arteritis virus. | 1976 | 183352 | |
| [influenza a/equine 2: example for an atypical course in a group of 21 horses]. | 1976 | 188127 | |
| viral respiratory infections. | 1976 | 177853 | |
| growth of equine arteritis virus in cells derived from infectious canine hepatitis virus-induced hamster tumor and transformed cells. | 1976 | 181624 | |
| studies on equine viral arteritis. ii. a serological survey of equine viral arteritis in horses imported in 1973/74. | 1976 | 181627 | |
| isolation of c1q-binding immune complexes by affinity chromatography and desorption with a diaminoalkyl compound. | the applicability of affinity chromatography to the isolation of c1q-binding immune complexes (ic) in sera was explored. purified human c1q was covalently coupled to agarose or adsorbed to igg-agarose resins. sera containing preformed virus-antibody complexes or rheumatoid arthritis (ra) sera were passed through the columns and c1q-bound ic, eluted off with 1,4-diaminobutan at mild basic conditions, were analysed by immunodiffusion, crossed immunoelectrophoresis, gel filtration and electron micr ... | 1976 | 773099 |
| hemagglutination by equine infectious anemia virus. | equine infectious anemia (eia) virus which was propagated on an equine dermal cell line agglutinated guinea pig erythrocytes. viral fluids containing about 10(7.5) mean tissue culture infective doses/ml showed hemagglutinating (ha) titers ranging from 16 to 32 units/0.05 ml. results of cesium chloride equilibrium density gradient centrifugation revealed that the hemagglutinin was inseparable from the virus particles. the hemagglutination reaction persisted over a wide range of temperature and ph ... | 1976 | 9361 |
| complement-fixation reactions in equine viral arteritis. | 1977 | 202179 | |
| [purification of equine influenza virus a/equi-2/w/9/69 by the sucrose step density gradient ultracentrifugation]. | 1978 | 213662 | |
| results of an epidemiological investigation on viral arteritis in france and some other european and african countries. | an original microplate seroneutralization technique was developed in order to study the existence of antibodies against equine viral arteritis. the technique involves a high amount of complement, and has allowed to demonstrate antibodies in 18.5% out of 4,037 horses examined. titers varied not or little during periods as long as 6 years. among 3,324 sera samples from french horses, a 15.2% frequency of antibodies was shown. the infection level was not very different between breeds, which is diff ... | 1978 | 213008 |
| a serologic survey on equine influenza for the past ten years. | 1978 | 212632 | |
| epidemiology of equine upper respiratory tract disease on standardbred racetracks. | the outbreaks of upper respiratory tract infections in horses at standardbred racetracks were investigated over a three year period. the most serious epidemics of respiratory disease occurred in the winter and spring seasons. both influenza viruses and equine herpesvirus 1 were shown to be present in the horse population. the herpesvirus was associated with respiratory disease particularly in the winter but the equine influenza viruses apparently were responsible for the major epidemics of respi ... | 1979 | 218705 |
| prevalence of antibodies to equine viruses in the netherlands. | the prevalence of antibodies to various viruses was investigated in a series of serum samples collected from horses in the netherlands between 1963 and 1966 and from 1972 onwards. neutralizing antibodies to equine rhinopneumonitis virus, equine arteritis virus and to equine rhinovirus types 1 and 2 were detected in respectively 76%, 14%, 66% and 59% of the equine serum samples tested. the observed incidence of serum samples positive to equine adenovirus in the complement fixation test was 39%. p ... | 1979 | 219560 |
| viral respiratory disease. | 1979 | 228463 | |
| prevalence of antibodies to equine viruses in the netherlands. | summary the prevalence of antibodies to various viruses was investigated in a series of serum samples collected from horses in the netherlands between 1963 and 1966 and from 1972 onwards. neutralizing antibodies to equine rhinopneumonitis virus, equine arteritis virus and to equine rhinovirus types 1 and 2 were detected in respectively 76%, 14%, 66% and 59% of the equine serum samples tested. the observed incidence of serum samples positive to equine adenovirus in the complement fixation tes ... | 1979 | 22039753 |
| temperature-sensitive mutants of equine arteritis virus. | seventeen temperature-sensitive mutants of equine arteritis virus, a nonarthropod-borne togavirus, have been isolated. 5-fluorouracil, o-methylhydroxylamine and ethyl methanesulphonate were used as mutagens. the mutants were characterized by their ability to synthesize virus rna and virus proteins at the permissive (35 degrees c) and restrictive temperature (40 degrees c) using autoradiography of cells labelled with 3h-uridine in the presence of actinomycin d and immunofluorescence respectively. ... | 1980 | 6252293 |
| pathologic features of horses given avirulent equine arteritis virus intramuscularly. | twenty horses that were seronegative for equine arteritis virus antibodies were inoculated im with live equine arteritis virus vaccine. the inoculation did not cause clinical signs of disease. a mild, transient febrile reaction developed in 6 horses, 3 of which were in poor condition before inoculation. six horses, 2 of which were in poor condition before inoculation, experienced mild lymphopenia. necropsy revealed mild lesions in the lymph nodes of 6 horses (3 of which were in poor condition be ... | 1981 | 6267970 |
| natural equine viral arteritis in foals. | 1981 | 6274007 | |
| stability of viability and immunizing potency of lyophilized, modified equine arteritis live-virus vaccine. | the bucyrus strain of equine arteritis virus, previously modified to avirulence and vaccinal virus by 131 serial passages in primary cell cultures of horse kidney followed by 111 passages in primary cell cultures of rabbit kidney, was further passaged in cultures of the e. derm (nbl-6) cell line, a continuous diploid cell line. pools of the 16th and 25th passages of the virus in this last equine dermal cell line were lyophilized and stored in lots at 37 c, 23 to 28 c, 4c, and -20 c. the viabilit ... | 1981 | 6275755 |
| equine arteritis virus-infected cells contain six polyadenylated virus-specific rnas. | 1982 | 6283728 | |
| effect of mycoplasma orale on growth of equine arteritis virus in vero cells. | 1982 | 6304392 | |
| antigenic comparison of equine arteritis virus (eav) and lactic dehydrogenase virus (ldv); binding of staphylococcal protein a to the nucleocapsid protein of eav. | 1983 | 6191473 | |
| pathology of maternal genital tract, placenta, and fetus in equine viral arteritis. | six pregnant mares were given equine viral arteritis virus intravenously. tissues from genital tracts, placentae, and fetuses were examined by light and electron microscopy to study the mechanism of abortion. four mares which died with acute disease had diffuse vacuolation of endometrial epithelium and systemic necrotizing vasculitis. two of these mares had dead fetuses and two had live fetuses; virus was isolated from tissues of one live fetus. placentae of mares dying from acute disease did no ... | 1984 | 6328724 |
| [equine viral arteritis: detection of antibodies of horses in argentina]. | 1984 | 6091374 | |
| [current virus diseases in horses. diseases in foals and respiratory tract infections]. | at the moment, horse praxis is confronted by two disease complexes which are difficult to fight against as well in prophylaxis as in therapy, but which get an increasing importance. first they concern virus infections of the foals and second primary virus-caused respiratory diseases. foals get infected during the embryonal/fetal development, in the perinatal or postnatal period. normally the infection is caused by latent infected, clinical healthy mares, or in the postnatal period by ubiquitous, ... | 1984 | 6098971 |
| [serologic studies on the occurrence of the arteritis virus in the horse in west germany]. | 1985 | 3002746 | |
| preparing for equine arteritis. | 1985 | 2983980 | |
| intracellular equine arteritis virus (eav)-specific rnas contain common sequences. | equine arteritis virus (eav) is a nonarthropod-borne togavirus. six virus-specific rna species have been found in eav-infected cells having the following molecular weights: 4.3 x 10(6) (rna1), 1.3 x 10(6) (rna2), 0.9 x 10(6) (rna3), 0.7 x 10(6) (rna4), 0.3 x 10(6) (rna5), and 0.2 x 10(6) (rna6). rna1 comigrates with the viral genome (m. f. van berlo, m. c. horzinek, and b. a. m. van der zeijst, 1982, virology 118, 345-352). all rnas hybridized with a radio-labeled cdna probe representing rna6, i ... | 1986 | 3014727 |
| responses of horses vaccinated with avirulent modified-live equine arteritis virus propagated in the e. derm (nbl-6) cell line to nasal inoculation with virulent virus. | nineteen horses with no prior experience with equine arteritis virus (eav) were inoculated im with an avirulent live-virus vaccine against equine viral arteritis; the vaccinal virus had been passaged serially 131 times in primary cell cultures of equine kidney, 111 times in primary cell cultures of rabbit kidney, and 16 times in an equine dermis cell line (eav hk-131/rk-111/ed-16). three or 4 of the vaccinated horses each, along with appropriate nonvaccinated controls, were inoculated nasally wi ... | 1986 | 3021027 |
| equine arteritis virus-induced polypeptide synthesis. | intracellular virus-specific proteins induced by equine arteritis virus (eav) have been compared with in vitro translation products of virion and intracellular eav rnas. in infected bhk-21 cells, the two major virion proteins (c and e1) and polypeptides with mol. wt. of 60,000 (p60), 42,000 (p42) and 30,000 (p30) were found. there were no indications that the viral proteins were processed from a larger precursor as shown by pulse-chase, amino acid analogue and protease inhibitor experiments. the ... | 1986 | 2426393 |
| identification and antigenic comparison of equine arteritis virus isolated from an outbreak of epidemic abortion of mares. | 1986 | 2431561 | |
| equine viral arteritis: a disease of emerging significance? | 1986 | 3015585 | |
| transmissibility and abortogenic effect of equine viral arteritis in mares. | a group of 14 pregnant mares was exposed via contact to 4 mares bred to stallions infected with equine viral arteritis virus. there was a demonstrable febrile response in each donor mare and in 12 of the pregnant mares. all 18 mares became seropositive after exposure. equine viral arteritis virus was isolated from the nasopharynx of 5 pregnant mares, but not from the donor mares. ten of the pregnant mares aborted, and virus was isolated from fetal specimens or placenta of 8. | 1986 | 3021696 |
| demonstration of the carrier state in naturally acquired equine arteritis virus infection in the stallion. | the chronic carrier state was virologically confirmed in 15 thoroughbred stallions naturally infected with equine arteritis virus based on the results of test matings and, or, isolations of the virus from semen. carrier stallions were shown to shed equine arteritis virus in the semen for at least one to two years. existence of a short-term or convalescent carrier state was also demonstrated in five additional stallions. the frequency of the long-term carrier state in stallions naturally infected ... | 1986 | 3022363 |
| translation of equine arteritis virus rna in rabbit reticulocyte lysates. | 1987 | 3035263 | |
| status of equine viral arteritis in kentucky, 1985. | clinical cases of equine arteritis virus infection have not been diagnosed in kentucky since 1984, and there has been no indication that any of the horses involved in the 1984 epizootic have since been responsible for spread of the disease to horses in other states or other countries. cases of abortion caused by naturally acquired infection with this virus have not been confirmed in 1984 or 1985. neither field nor vaccine strains of equine arteritis virus have been shown to induce teratologic ab ... | 1987 | 3038806 |
| [serologic follow-up studies of viral arteritis in horses at a stud farm]. | 1987 | 2823506 | |
| the carrier state in equine arteritis virus infection in the stallion with specific emphasis on the venereal mode of virus transmission. | the carrier state has been confirmed virologically in thoroughbred and non-thoroughbred stallions naturally infected with equine arteritis virus (eav). short-term or convalescent and long-term carriers occur. the frequency rate of the long-term carrier state in thoroughbreds was high, averaging 33.9% among the three groups of stallions under study. while the convalescent carrier state only lasted a few weeks after clinical recovery, the long-term carrier state could persist for years. there was ... | 1987 | 2824772 |
| structural polypeptides of equine arteritis virus. | 1987 | 2824911 | |
| status of equine viral arteritis in kentucky for 1986. | 1987 | 3035776 | |
| [respiratory infectious diseases in horses]. | among all infectious diseases affecting horses, respiratory disease pose the greatest threat to horses kept in stables, horses used for breeding and race horses. here a distinction should be made between the so-called monocausal infectious diseases (so-called henle-koch postulates) and multicausal infectious diseases which are the result of the synergistic interaction of different processes, that alone do not lead to disease. there is no clearcut distinction between the two groups. the most impo ... | 1987 | 3296310 |
| equine viral arteritis. | equine viral arteritis is reviewed with specific reference to clinical features, etiology, transmission, diagnosis, epidemiology, and current methods for the control of this disease. there is evidence of variation in pathogenicity among strains of equine arteritis virus. virus transmission occurs primarily by the respiratory and venereal routes during the acute phase of the infection. the long-term carrier stallion appears to play a major epidemiological role in dissemination and perpetuation of ... | 1987 | 17422919 |
| ontario. equine arteritis virus isolated from a standardbred foal with pneumonia. | 1988 | 17423174 | |
| serological studies concerning equine arteritis virus infection in the german democratic republic. | 1988 | 2840046 | |
| equine viral arteritis may have been introduced. | 1988 | 2851969 | |
| aetiology of kawasaki disease. | thirteen serum samples from nine children with kawasaki disease and 23 control samples gave negative results on screening for antibodies to hog cholera virus, border disease of sheep, bovine diarrhoea virus, and equine arteritis virus. the sera from two children with kawasaki disease were cytotoxic; a possible link with cytotoxin from propionibacterium acnes is considered. | 1989 | 2539788 |
| the effects of vaccination with tissue culture-derived viral vaccines on detection of antibodies to equine arteritis virus by enzyme-linked immunosorbent assay (elisa). | an enzyme-linked immunosorbent assay (elisa) was developed for the detection of serum antibodies to equine arteritis virus (eav). results from this assay produced a good correlation with results from virus neutralisation tests in horses which had not been regularly vaccinated with commercially available mammalian tissue culture-derived viral vaccines. vaccination of some horses with tissue culture-derived vaccines induced the formation of antibodies to bovine serum. these antibodies reacted with ... | 1989 | 2773278 |
| phylogenetic distribution of the novel avian endogenous provirus family eav-0. | a new family of related endogenous proviruses, existing at 50 to 100 copies per haploid genome and distinguishable by remarkably short long terminal repeats, has been described for domestic chickens (gallus gallus subsp domesticus). in this communication, by using southern blot analysis and probes derived from both internal viral sequences and locus-specific, cellular flanking sequences, we studied the genetic distribution of this family of moderately repetitive avian endogenous retroviruses wit ... | 1990 | 2398526 |
| [equine viral arteritis]. | equine viral arteritis (eva) caused by the equine arteritis virus, member of the genus arterivirus within the family of togaviridae was recently isolated from the seminal plasma of two stallions indicating that the virus infection is also prevalent in the federal republic of germany despite the apparent lack of acute clinical symptoms in the horse population. these findings are further supported by data from serological screenings. out of 739 horse sera, 28 (3.8%) were found to have eva virus-sp ... | 1990 | 2165641 |
| equine viral arteritis. | 1990 | 1963771 | |
| the occurrence of equine arteritis virus in australia. | this paper reports the first isolation of equine arteritis virus (eav) in australia and serological evidence of exposure to eav in australian horses. twelve standardbred stallions imported from north america were found to shed eav in semen. one hundred and seven stallions were tested for serum antibodies to eav and 73% of standardbred stallions tested were seropositive as compared to 8% of thoroughbred stallions. serum antibody was detected in 71% of standardbred mares, 6% of standardbred raceho ... | 1990 | 1963772 |
| reverse transcription and cdna amplification by the polymerase chain reaction of equine arteritis virus (eav). | a technique is described for the amplification and specific identification of equine arteritis virus (eav) nucleotide sequences. the polymerase chain reaction (pcr) was evaluated initially by amplification of cloned virus specific cdna sequences prior to amplification of single-stranded (ss) cdna produced by reverse transcription (rt) of viral genomic rna. three separate primer pairs were used for rt/pcr of eav genomic rna, each pair producing only one band in agarose gels of the predicted size ... | 1990 | 1702094 |
| induction of immune response and protection from equine viral arteritis (eva) by formalin inactivated-virus vaccine for eva in horses. | thirty-nine horses included 3 pregnant mares were examined by inoculating with formalin inactivated-virus vaccine for eva. antibody response of horses after one dose vaccination was somewhat poor and 50% effective inoculum dose of the vaccine should be included 10(8.4) pfu of virus before inactivation. after 2 doses given at an interval of 4 weeks, the horses developed such high titer of sn antibody as up to 1:5,120. the sn titer declined rather rapidly, but supplemental administration of the va ... | 1990 | 2163579 |
| all subgenomic mrnas of equine arteritis virus contain a common leader sequence. | during the replication of equine arteritis virus (eav) six subgenomic mrnas are synthesized. we present evidence that the viral mrnas form a 3'-coterminal nested set and contain a common leader sequence of 208 nucleotides which is encoded by the 5'-end of the genome. the leader is joined to the bodies of mrna 5 and 6 at positions defined by the sequence 5' ucaac 3'. the part of the leader sequence flanking the ucaac motif is very similar to the 5'-splice site of the tetrahymena pre-rrna. a possi ... | 1990 | 2162519 |
| viral respiratory disease of the horse. | the diagnosis of any viral respiratory disease relies on laboratory procedures to isolate the virus and demonstrate a significant rise in serum antibody titers. to isolate viruses from the upper respiratory tract, it is imperative that nasopharyngeal swabs are obtained from animals in the early acute stage of illness, i.e., during the pyrexic phase when the virus is replicating. nasopharyngeal swabs must be placed in a virus transport medium and forwarded immediately to the laboratory at refrige ... | 1991 | 1647261 |
| detection of cattle infected with bovine viral diarrhea virus using nucleic acid hybridization. | a ribonucleic acid (rna) hybridization assay to identify cattle infected by bovine viral diarrhea virus (bvdv) is described. the rna probe was derived from the coding region at the 3' end of the genome of the nadl strain of bvdv. total rna from infected cell cultures or peripheral blood leukocytes from suspect animals was extracted and applied to nylon membranes with a slot blot apparatus. peripheral blood leukocytes were tested concurrently for bvdv by virus isolation. the results of hybridizat ... | 1991 | 1645592 |
| arteritis in equine fetuses aborted due to equine viral arteritis. | 1991 | 1650052 | |
| a nested set of eight rnas is formed in macrophages infected with lactate dehydrogenase-elevating virus. | total rna was extracted from primary cultures of mouse macrophages isolated from 10-day-old mice 6 to 12 h postinfection with lactate dehydrogenase-elevating virus (ldv). poly(a)+ rna was extracted from spleens of 18-h ldv-infected mice. the rnas were analyzed by northern (rna) blot hybridization with a number of ldv-specific cdnas as probes. a cdna representing the nucleocapsid protein (vp-1) gene located at the 3' terminus of the viral genome (e. k. godeny, d. w. speicher, and m. a. brinton, v ... | 1991 | 1870216 |
| equine arteritis virus is not a togavirus but belongs to the coronaviruslike superfamily. | the nucleotide sequence of the genome of equine arteritis virus (eav) was determined from a set of overlapping cdna clones and was found to contain eight open reading frames (orfs). orfs 2 through 7 are expressed from six 3'-coterminal subgenomic mrnas, which are transcribed from the 3'-terminal quarter of the viral genome. a number of these orfs are predicted to encode structural eav proteins. the organization and expression of the 3' part of the eav genome are remarkably similar to those of co ... | 1991 | 1851863 |
| [serological studies of the recent infections of austrian horses with the equine arteritis virus]. | 944 serum samples of horses, collected in 1988 and 1989, were examined for the occurrence of antibodies against equine arteritis virus by a microneutralizations test. in 10.9% of all sera reactors could be found. the distribution of seropositive horses varied from 4.6% (salzburg) to 15.7% (lower austria). from tyrol and vorarlberg no samples could be obtained. it was not possible, to correlate clinical symptoms (infertility, respiratory symptoms, fever and edema) with the infection. it is assume ... | 1991 | 1851082 |