TitleAbstractYear(sorted ascending)
epizootiology of bovine rotavirus infection.published information on rotaviruses as pathogens, the source of virus infection and the method of transmission of infection under normal conditions are reviewed. the antigenic differences between rotavirus isolates from children, calves, pigs, foals and mice are discussed. bovine rotaviruses isolated in the usa and the uk were shown to be closely related antigenically and the us vaccine strain protected calves from challenge with the uk rotavirus. nineteen normally reared calves, with 20 or mor ...1978211696
antibody titres to lamb rotavirus in colostrum and milk of vaccinated ewes.ewes were vaccinated two to three weeks prior to mating with a formalin-treated preparation of lamb rotavirus. the colostrum and milk produced by vaccinated ewes after the subsequent pregnancy were shown to contain significantly higher titres of antibody to the virus than did mammary secretions from non-vaccinates. the virus neutralising antibody activity was associated with igg in both colostrum and milk. however, igg concentrations in the mammary secretions of vaccinates and non-vaccinates did ...1978211697
rotavirus in goats. 1978211701
immune response to rotaviral infection--measurement by enzyme immunoassay. 1978212390
panel report on the colloquium on selected diarrheal diseases of the young. 1978212391
naturally occurring and experimentally induced rotaviral infections of domestic and laboratory animals. 1978212392
rotaviral infections in human neonates.between may 1, 1976 through may 14, 1977, feces from 1,056 five-day-old babies in newborn nurseries were examined by electron microscopy, of which 343 (32.5%) contained rotavirus. rotaviruses were prevalent throughout the study period, but infection reached its peak during the winter months of december 1976 and january 1977, at which time 46% and 49% of babies, respectively, were excreting rotaviruses. infection occurred substantially less frequently among breast-fed babies. further, infected br ...1978212393
detection of differences among human and animal rotaviruses, using analysis of viral rna. 1978212394
electron microscopy in the diagnosis of infectious diarrhea. 1978212395
pathogenesis of rotaviral infection. 1978212396
comments on the pathogenesis of rotaviral infections. 1978212397
techniques for rotaviral propagation. 1978212398
studies on rotaviral antibody in bovine serum and lacteal secretions, using radioimmunoassay. 1978212399
passive immunity in rotaviral infections. 1978212400
comments on passive immunity in rotaviral infections. 1978212401
immunization of infants and young children against rotaviral gastroenteritis--prospects and problems. 1978212402
economic impact of rotavirus and other neonatal disease agents of animals.methods for estimating the economic impact of disease agents were developed and utilized to assess the relative economic importance of rotavirus and other disease agents in calves. based on incidence data from 2 sources, escherichia coli was responsible for the most devastating economic losses (50.9% and 74.6%). coronaviral (17.5% and 29.7% loss) and rotaviral (3.2% and 9.1% loss) infections ranked 2nd and 3rd, respectively. in one study, cryptosporidial infections (6.5% loss) were estimated to ...1978212403
pathogenic relationships of rotavirus, escherichia coli, and other agents in mixed infections in calves.infection with agents interpreted as causing or contributing to diarrhea (rotavirus, coronavirus, enterotoxigenic escherichia coli, and cryptosporidia) were demonstrated in 24 of 32 newborn calves that had naturally occurring diarrheal disease. the calves were from 12 herds in iowa. infections as well as enteric lesions and hypoglobulinemia occurred more frequently among diarrheal calves than among nondiarrheal calves from these same herds. in most calves, infections were mixed; ie, both viruses ...1978212404
clinical management and control of neonatal enteric infections of calves. 1978212411
comparison of an enzyme-linked immunosorbent assay for quantitation of rotavirus antibodies with complement fixation in an epidemiological survey.the development of a micro-scale enzyme-linked immunosorbent assay (elisa) with horseradish peroxidase as the marker enzyme for the detection and measurement of human rotavirus antibodies is described. a semipurified preparation of the serologically related simian agent, sa-11 virus, was used as the antigen. test sera were reacted with antigen-sensitized wells in disposable poly-vinyl microplates. any attached antibody was detected by the addition of peroxidase-labeled anti-species immunoglobuli ...1978212451
measurement of rotavirus antibody by an enzyme-linked immunosorbent assay blocking assay.a new method for the measurement of rotavirus antibody is described, utilizing the system of enzyme-linked immunosorbent assay (elisa). in this method, serum is incubated with a fixed amount of rotavirus antigen, and the amount of antibody is determined by measuring the amount of unneutralized antigen. such an assay system proved to be as efficient as the other available rotaviral antibody systems. the elisa blocking assay also has the advantages of not requiring purified or gnotobiotic antigen ...1978212453
a search for faecal viruses in new-born and other infants.faecal specimens were collected at weekly intervals over the winter months from 141 new-born infants without diarrhoea. contrary to the findings in other studies, no viruses were detected by electron micriscopy or culture in any of these specimens. over the same period faecal specimens were collected from 84 infants up to four years of age admitted to hospital. rotaviruses or adenoviruses were found in 48% of infants with gastroenteritis. enteroviruses and other small round virus-like particles ...1978212477
stool viruses in babies in glasgow 2. investigation of normal newborns in hospital.the stools of 37 newborn babies born in hospital were examined for the presence of virus. an extract of every stool passed by each baby was examined in the electron microscopy and inoculated into cell cultures. the babies were delivered in four separate maternity units (a-d). all the babies from unit a and c(9 babies) were found to be excreting rotavirus though none showed any evidence of diarrhoea. two of the babies also excreted astrovirus. subsequently unit a was closed for cleaning and, on r ...1978212478
summary of a workshop on new and useful methods in viral diagnosis. 1978212491
human rotavirus infection in infants and young children with intussusception.human rotavirus was detected by electron microscopy in 11 of 30 infants and young children with intussusception (37% of subjects under study). serologic complement fixation tests revealed evidence of infection with the rotavirus in 70% of the patients examined who eliminated the rotavirus in their stools. these results indicate that human rotavirus, in addition to adenovirus, may be an infectious agent causing intussusception in infants and young children.1978212529
[virus diarrhea in calves in south africa].the importance of diarrhoea in calves is briefly discussed. the role of viruses as primary invaders of the intestinal epithelium is stressed. the pathogenesis and problems encountered in diagnosing virus associated diarrhoea as well as the morphology of rota and coranavirus are discussed. possible methods of prevention and treatment are briefly mentioned.1978212561
epidemiology of human rotavirus types 1 and 2 as studied by enzyme-linked immunosorbent determine the relative importance of two known serotypes of human rotavirus, we developed an enzyme-linked immunosorbent assay to differentiate serotype-specific rotavirus antigen and antibody. using this technic, we studied the epidemiology of the two serotypes in acute gastroenteritis. seventy-seven per cent of 414 rotavirus isolates were type 2, and the remainder were type 1. the serotype distribution was similar in specimens from children in washington, d.c., and other parts of the world. ...1978212678
rotavirus diarrhoea of foals. 1978213048
concurrent rotavirus and salmonella infections in foals. 1978213413
a long-term survey of rotavirus infection in japanese children with acute gastroenteritis.human rotavirus was detected by electron microscopic examination of the stools of 320 (63%) of 506 infants and young children hospitalized with acute gastroenteritis between december 1974 and march 1977. serologic responses to infection with the rotavirus were revealed by the complement-fixation test in 130 (70%) of 185 patients examined. during the study period three epidemics of human rotavirus infection occurred during the winter months. the peak incidences occurred in january 1975 (88% of pa ...1978213501
an investigation into the possible role of the family unit in the transmission of rotavirus infections of children. 1978213802
rotavirus infection in avian species. 1978213873
[coronavirus and "astrovirus" observed in stools of children with gastroenteritis (author's transl)].in a year's survey (december 1976-november 1977) of 190 children admitted to hospital with acute gastroenteritis, an "aetiological" agent was found in stools by electron microscopy in 41% of cases. rotavirus were found in 34% of patients. we also found coronavirus-like particles and astrovirus-like particles in 25 and 5 stools, respectively. improvements are awaited to demonstrate the responsibility of these virus in gastroenteritis.1978214002
acute infantile gastroenteritis caused by rotavirus in japan.we had an outbreak of acute infantile gastroenteritis accompanied by milky-white stool (called hakuri in japanese) during the winters of 1976 and 1977. stool specimens collected from 72 cases of hakuri were studied by negative-staining electron microscopy. rotavirus was detected with a very high frequency (89%). rotavirus obtained from one of the patients was isolated and passaged in cultures of primary human embryonic kidney cells. viral antigens could be detected in the cytoplasm of the cells ...1978214305
role of rotavirus (reo-like) in weanling diarrhea of pigs.piglets weaned abruptly and precociously at 3 weeks of age and placed in a crowded nursery commenced diarrhea 3 to 5 days later. death losses were low (approximately 6%), but weight gain ceased for 2 weeks. large numbers of rotavirus (reo-like) particles were seen by electron microscopy in diarrhetic fluids. sections of intestines showed a loss of adsorptive surface in that villi were shortened and fused with adjacent villi. immunofluorescence revealed rotaviral antigens within damaged enterocyt ...1978214458
secretory antibody directed against rotavirus in human milk--measurement by means of enzyme-linked immunosorbent assay.human milk contains antibodies to a variety of enteropathic agents. we utilized the method of enzyme-linked immunosorbent assay to investigate anti-rotavirus secretory iga in 113 human milk and colostral specimens from a rural area in guatemala, 32 colostral specimens from an urban area of costa rica, and 12 from an urban area of the united states. anti-rotavirus sciga was found in all colostral samples and in 94% of the milk specimans. both the absolute concentration of anti-rotavirus sciga and ...1978214534
nosocomial infantile gastroenteritis associated with minirotavirus and calicivirus. 1978214535
detection of antibody to rotavirus by counterimmunoelectrophoresis in human serum, colostrum, and milk. 1978214536
jejunal microbial flora of southern indian infants in health and with acute gastroenteritis.the microbial flora of the jejunal lumen of 28 infants with acute gastroenteritis was compared with that of a group of 10 normal infants. the jejunum of control subjects harboured an "oral" type of flora and in a few instances enterobacteria in small numbers. the concentrations of all but one of the groups of organism were higher in the patients than in controls, and the differences were of statistical significance for enterobacteria and lactobacilli. in eight subjects, the same pathogen was ide ...1978214565
isolation of a reovirus-like agent (rotavirus) from neonatal calf diarrhea in japan. 1978214670
comparison of five diagnostic methods for the detection of rotavirus antigens in calf faeces.immunoelectroosmophoresis, complement fixation, immunofluorescence on inoculated cell cultures, electron microscopy, and an enzyme-linked immunosorbent assay were compared for the detection of rotavirus in faecal samples from calves. rotavirus particles could be detected in 39 out of 98 faecal samples by electron microscopy. immunofluorescence, complement fixation, and immunoelectroosmophoresis detected rotavirus antigens in 39, 42, and 30 samples, respectively. the enzyme-linked immunosorbent a ...1978214679
rotavirus infections in families. a clinical and virological study.among 25 family members of 8 children with acute rotavirus gastroenteritis (8 siblings and 17 adults) gastroenteritis was recorded in 9 (5 siblings and 4 adults), and minor symptoms in 8 (2 siblings and 6 adults). a rotavirus infection was diagnosed in 7 of the family members with gastroenteritis and was probably the cause of the disease in the remaining 2 in this group. four of the 8 family members with minor symptoms were infected with rotavirus, whereas no infection was detected among the 8 f ...1978214850
a study on the ultrastructure of human rotavirus. 1978214956
viral diarrhea. etiology and control. 1978215028
synthesis of coreless, probably defective virus particles in cell cultures infected with cells infected with pig and lamb rotavirus strains which were not adapted to serial growth in cell cultures were examined by electron microscopy. a major difference between virus morphogenesis in the initial passage in pk-15 cells and in intestinal epithelial cells was the generation of large numbers of coreless virus particles in pk-15 cells. the numbers of coreless particles increased with increasing multiplicity of infection. infectious virus was synthesized in pk-15 cells, but a variab ...1978215104
isolation of rotavirus from foals with diarrhoea.a rotavirus, morphologically similar to other known rotaviruses, was demonstrated in the faeces of 5 foals with diarrhoea on two properties. four of these 5 samples produced specific intracytoplasmic fluorescence in cell culture when reacted with calf rotavirus antiserum conjugate. sixteen affected foals from both properties were depressed, did not suckle and became recumbent. most had a watery diarrhoea which lasted for 3 days and resulted in some dehydration and loss of body condition. sick fo ...1978215112
physicochemical properties of nebraska calf diarrhea virus hemagglutinin.highly purified nebraska calf diarrhea virus (ncdv) was prepared by cesium chloride density gradient centrifugation. the effect of temperature, ph, different concentrations of formaldehyde, chloroform, ether, ethyl alcohol, and methyl alcohol on ncdv hemagglutinin and virus morphology was studied. ncdv hemagglutinin was inactivated by temperature, ph 2.0, chloroform, ethyl alcohol, and methyl alcohol.197833758
neonatal calf diarrhea caused by a virus that induces villous epithelial cell syncytia.intestinal lesions caused by a virus serologically unrelated to the calf diarrheal rotavirus or coronavirus were studied in gnotobiotic calves. the virion purified from feces from infected calves was a fringed particle with a diameter of about 100 nm. the incubation period from time of inoculation per orum to onset of diarrhea in calves was as short as 8 hours. the viral infection in bacteria-free calves or calves not contaminated with pathogenic bacteria caused severe illness for only 24 hours. ...197898080
pattern of shedding of two noncultivable viruses in stools of newborn babies.noncultivable viruses have been associated with diarrhea affecting newborn babies in obstetric hospital nurseries. persisting infection in a special care nursery in melbourne, australia, permitted a study of the pattern of excretion of these viruses. ten babies admitted to the nursery within 2 hr of birth were randomly selected for prospective study. feces were collected daily for 14 days and were examined by electron microscopy. all ten babies excreted detectable amounts of duovirus (rotavirus, ...197898612
different serotypes of human rotaviruses. 197874513
serotypes of human rotavirus. 197874514
anti-rotavirus antibody in human colostrum. 197874515
rotavirus infection in high-incidence lactase-deficiency population. 197875362
anti-rotavirus antibody in human colostrum. 197876105
rotavirus infections of neonates. 197876780
recurrent rotavirus gastroenteritis. 197876904
the rotaviruses. 197877663
asymptomatic endemic rotavirus infections in the newborn.between may 1, 1976, and may 14, 1977, 343 (32.5%) of 1056 5-day-old babies in newborn nurseries excreted rotaviruses. the infection-rate was highest during winter (49%). 76% of infected babies at this time were bottle-fed. 41% of neonates excreted low amounts of virus (less than or equal to 10(8) particles/g faeces); older children tended to excrete greater than 10(10) particles/g faeces. infected breast-fed babies excreted less virus than those who were bottle-fed. stools of breast-fed babies ...197877944
sequential enteric illnesses associated with different rotavirus serotypes. 197878225
comparison of sucrose with glucose in oral therapy of infant a randomised double-blind trial, 51 5--10% dehydrated infants were rehydrated with oral electrolyte solutions containing sucrose or glucose. most infants in both groups were successfully rehydrated, but the sucrose solution produced a slower correction of electrolyte abnormalities and a higher percentage of patients who needed more than 24 h of therapy. where there is adequate knowledge of the oral therapy method sucrose can substitute for glucose in many cases; where there is a choice glucos ...197879080
sugar intolerance in rotavirus gastroenteritis. 197879824
rotavirus infections of apes. 197879844
rotaviruses. 197880441
secretory iga antibody to rotavirus in human milk 6--9 months postpartum. 197880554
more serotypes of human rotavirus. 197880556
search by immunofluorescence for antigens of rotavirus, pseudomonas maltophilia, and mycobacterium kansasii in crohn's disease.crohn's-disease tissue was tested by indirect immunofluorescence for antigens of rotavirus, pseudomonas maltophilia, and mycobacterium kansasii. no reactions were obtained with anti-serum to p. maltophilia and m. kansasii, and a granular fluorescence seen with rotavirus antibody was probably non-specific.197880631
classification of rotaviruses: report from the world health organization/food and agriculture organization comparative virology program.the reoviridae working team was established under the world health organization/food and agriculture organization comparative virology program in 1975. the generic name rotavirus has been adopted for the reovirus-like agents associated with diarrhea in man and animals, and the nebraska calf diarrheal virus strain of bovine rotavirus has been selected as a candidate reference virus. stocks of this virus and of gnotobiotic calf antiserum have been prepared. antigenic differences among rotaviruses ...197881198
location of type-specific antigens in calf rotaviruses.rotavirus antisera with and without neutralizing activity to calf rotavirus were compared for their ability to agglutinate calf rotavirus particles with and without an outer capsid layer. particles without the outer capsid layer were agglutinated by antisera with immunofluorescent antibody activity and by antisera with immunofluorescent antibody and neutralizing activity. particles with the outer capsid layer were agglutinated only by antisera with neutralizing activity. the neutralization test ...197884819
hemagglutination and hemagglutination-inhibition studies with a strain of nebraska calf diarrhea virus (bovine rotavirus).a hemagglutinin has been prepared from nebraska calf diarrhea virus (ncdv) propagated in bs-c-1 cell line. after cesium chloride centrifugation, the hemagglutinin of the bovine rotavirus was found to be associated with intact virions (density 1.355 g/ml) but not with virions lacking an outer capsid layer (density 1.375 g/ml). in hemagglutination-inhibition (hai) tests, the hemagglutinin reacted specifically with ncdv serum, and hai seroconversions were detected in some sera tested. cross-reactio ...1978201585
[epidemiological aspects of gastroenteritis dut to rotavirus].during december 1976 to january-march 1977, 13/53 children less than 5 years of age were admitted with acute enteritis caused by rotaviruses to the hospital del niño, dif. there is already convincing evidence that this new virus will prove to be the most important etiological agent of sporadic acute enteritis in young children.1978202294
a simple method for concentration of enteroviruses and rotaviruses from cell culture harvests using membrane compounds in cell culture harvests known as membrane-coating components (mcc) prevent virus adsorption to membrane filters. blending cell culture harvests with fluorocarbon removed the mcc and permitted adsorption of virus in acidified harvests to epoxy-fiberglass filters. subsequent elution with high ph buffer resulted in recovery of greater than 90% of the virus with concentrations of up to 100-fold.1978202572
the role of breast-feeding in the prevention of rotavirus infection.breast-fed infants are less susceptible to gastroenteritis than bottle-fed infants. antibodies against rotavirus, the major pathogen of infantile gastroenteritis, were sought in human sera, colostrum and milk specimens by immunofluorescence. an experimental murine-rotavirus model was established by infecting the second litters of dams 4 weeks after infecting their first litters. antibodies were absent from human and murine colostrum and milk specimens despite being present in virtually all sera, ...1978202710
rearing regimen producing piglet diarrhea (rotavirus) and its relevance to acute infantile diarrhea.piglets were weaned when 1 day old and thus were denied further access to the antibodies supplied by their dam's milk. they were placed in a nursery in which contamination by the ubiquitous rotavirus steadily increased with continuous use causing a progressive increase in the incidence of vomiting, diarrhea, and death among the piglets. a similar syndrome involving an antigenically related rotavirus and analogous management practices occurs in human infants.1978203032
[existence of 2 types of rotavirus associated with acute gastroenteritis in children].rotaviruses found in the stools of different infants, hospitalized in the hospital del niño del dif, may be grouped into at least two types according to their rna composition. the viral rna composition was analyzed by electrophoresis in agarose gels. the different types can be distinguished by the mobility of the second larger of the 11 segments that make up the viral rna.1978203294
[diagnosis of rotavirus using viral rna electrophoresis].rotavirus has emerged as a major agent of acute enteritis of infants. a rapid and simple technique has been developed for its detection, based on the visualization of the viral rna electrophoretic pattern. this technique takes advantage of the large amount of rotavirus present in the stools and the high sensitivity for detection of rna obtained with ethidium bromide.1978203295
mechanisms in the pathogenesis of diarrhea: a review. 1978203563
rotavirus as a cause of diarrhea in pigs.a rotavirus (reovirus-like agent) was associated with diarrheal diseases occurring in 1- to 4-week-old suckling pigs in 8 herds and in weaned pigs in 2 herds. transmissible gastroenteritis virus was also detected in 2 of these herds, as was enteropathogenic escherichia coli in 5 herds. morbidity was generally greater than 80% in pigs of the affected age group within these herds, and mortality from diarrhea ranged from 7 to 20%. the disease due to rotavirus in suckling pigs appeared similar to th ...1978203565
comparison between adsorption of poliovirus and rotavirus by aluminum hydroxide and activated sludge flocs.adsorption of poliovirus and rotavirus by aluminum hydroxide and activated sludge flocs was studied. both aluminum hydroxide and activated sludge flocs adsorbed greater amounts of poliovirus than rotavirus. aluminum hydroxide flocs reduced the titer of poliovirus in tap water by 3 log10, but they only reduced the titer of a simian rotovirus (sa-11) in tap water by 1 log10 or less and did not noticeably reduce the number of human rotavirus particles present in a dilute stool suspension. activated ...1978205173
comparison of rotavirus strains by hemagglutination inhibition.rotaviruses have been shown to be of importance as aetiologic agents of gastroenteritis in infants and in domestic animals of several species. hemagglutinins were prepared from two canadian isolates of bovine rotavirus and from one isolate of a simian rotavirus. a united kingdon isolate of bovine rotavirus was shown not to possess hemagglutinating activity, indicating a strain difference between a canadian and united kingdom bovine rotavirus. in hemagglutination-inhibition (hai) tests a rabbit h ...1978205329
a study of etiologic agents in infantile gastroenteritis. 1978205524
a simplified method for detecting fluorescent antibody to rotavirus: its application to sero-epidemiology. 1978205558
the immunoprophylaxis of of rotavirus infections in lambs.the effect of colostrum or serum feeding on subsequent rotavirus infections was investigated in neonatal gnotobiotic lambs. immunity after feeding colostrum did not depend on absorption of passively acquired antibody into the circulation. protection against clinical disease depended on the volume of colostrum ingested. the protection afforded by feeding serum was specific, since serum free of rotavirus antibody failed to confer protection. immune serum fed at a rate of 2.5 ml per kg twice daily ...1978205988
multiple viruses present in the faeces of a scouring calf. 1978205989
fatal rotavirus gastroenteritis: an analysis of 21 cases.during the period of may 1972 to march 1977, twenty-one fatal cases of rotavirus acute gastroenteritis were recorded in the city of toronto. the mean age of these subjects was approximately 1 year. boys outnumbered girls by 12 to 9. death occurred within three days of onset of symptoms in all cases. sixteen of the subjects were profoundly dehydrated and had sodium levels (serum or vitreous humor) in excess of 150 meq/liter. in 11 subjects, sodium values were greater than 160 meq/liter. although ...1978206132
lapine rotavirus: preliminary studies on epizoology and transmission.antibody to rotavirus was found in sera of domestic rabbits, wild cottontail rabbits and snowshoe hares. eight out of 187 stools from a rabbitry contained rotavirus, though no clinical symptoms were apparent. the virus extracted from one such stool infected a five week old seronegative rabbit and led to distention and congestion of the colon, excretion of virus in the stool and a serological response to virus. human and simian rotaviruses did not replicate in the rabbits.1978206325
characteristics of neonatal calf diarrhea virus ribonucleic acid.the nucleic acids of neonatal calf diarrhea virus were characterized by isopycnic centrifugation in cs2so4, electron microscopy, ultraviolet absorbance temperature profiles and polyacrylamide gel electrophoresis. these studies indicated that the neonatal calf diarrhea virus genome consists of 11 segments of double stranded rna with a total molecular weight of 10.75 million daltons.1978206326
diarrhea in newborn cynomologus monkeys infected with human rotavirus.of six newborn cynomolgus monkeys (macaca fascicularis) naturally delivered and normally nursed five developed diarrhea after oral administration of human rotavirus. virus excretion was observed in the stool of four animals. this virus was transmitted to four out of six other monkeys causing diarrhea in only one animal.1978206515
hemagglutination by simian rotavirus.a simian rotavirus (sa-11) was shown to hemagglutinate human group "o" and guinea pig erythrocytes. the hemagglutinin appeared to be associated with the outer capsid of the sa-11 virus and was inhibited by specific hyperimmune anti-sa-11 guinea pig serum.1978206575
morphogenesis of porcine rotavirus in porcine kidney cell cultures and intestinal epithelial cells.the morphogenesis of porcine rotavirus was similar in vitro in porcine kidney (pk) cell cultures and in vivo in porcine epithelial cells as examined by electron microscopy. infected cells contained cytoplasmic, non-membrane-bound viroplasm and accumulations of virus particles within cisternae of the rough endoplasmic reticulum (rer). three types of virus particles were noted: double-shelled or complete particles which averaged 77 nm in diam.; single-shelled or naked particles which ranged from 5 ...1978206653
central nervous system involvement in patients with rotavirus gastroenteritis.two children with rotavirus gastroenteritis are presented. the first case developed a fatal reye's syndrome and the other one encephalitis with slow recovery. the rotavirus diagnosis was made in both cases by electron microscopy and a significant rise in antibody titres to nebraska calf diarrhea virus was seen in one of the two patients.1978204984
[rotaviruses as pathogens for calf diarrhea: prevalence of complement fixing antibodies in fattening calves]. 1978204995
trypsin and bovine rotavirus replication. 1978205033
pathogenesis of porcine rotaviral infection in experimentally inoculated gnotobiotic pigs.porcine rotavirus was shown to infect gnotobiotic pigs and induce an acute enteric disease clinically characterized by diarrhea, anorexia, depression, and occasional vomition. onset of clinical signs correlated closely with the appearance of lesions within the small intestinal mucosa, and recovery from infection was associated with the regeneration of normal, functional villous epithelium. villous atrophy, especially in the caudal two-thirds of the small intestine, was the consistent lesion obse ...1978204232
calf rotavirus: detection of outer capsid glycoproteins by lectins. 1978204517
gradual reintroduction of full-strength milk after acute gastroenteritis in children.46 children (26 boys and 20 girls) admitted with mild acute gastroenteritis were randomly allocated to a regimen of continuing on full-strength milk, or to one of taking clear fluids until the diarrhoea settled before full-strength milk was reintroduced either immediately, or gradually in quarter-strength steps. there was no difference in length of hospital stay between the three groups.197986003
simian rotavirus (sa 11) in serodiagnosis of human rotavirus infections.tests were made on 169 sera from children up to 10 years of age for rotavirus antibodies by indirect immunofluorescence and complement fixation tests. the simian strain sa 11 served as a substitute antigen for the human rotavirus. furthermore, the sa 11 complement-fixing antigen was compared with a commercial antigen of the bovine rotavirus strain ncdv. it was demonstrated that sa 11 which can be more easily propagated than most bovine strains, may be used as a substitute antigen in complement f ...197986940
cell free transcription and translation of rotavirus rna. 197988940
the rotavirus syndrome. 197989290
isolation and cell culture propagation of rotaviruses from turkeys and chickens.rotaviruses were detected by electron microscopy in the faeces of turkey poults and broiler chickens with diarrhoea. apparently symptomless infection was also observed in broilers. the avian rotaviruses could not be isolated in cell cultures by conventional techniques, but were adapted to serial growth in chick cell cultures following trypsin treatment of the virus and the cells. immunofluorescence studies showed that the avian and mammalian rotaviruses are antigenically related. antibodies to r ...197992979
use of antisera against bovine (ncdv) and simian (sa11) rotaviruses in elisa to detect different types of human rotavirus.two elisa systems for the detection of human rotaviruses were developed. in the first system antibodies to nebraska calf diarrhea virus (ncdv) were used for coating the solid matrix and for the preparation of the enzyme conjugate. in the second system antibodies to human rotavirus and antibodies to simian rotavirus (sa11) were used for coating the solid matrix and for the preparation of the enzyme conjugate respectively. the second elisa system proved to have a broader spectrum for the detection ...197994274
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