TitleAbstractYear(sorted ascending)
release of progeny virus from cells infected with simian rotavirus sa11.analysis of cells infected with simian rotavirus sa11 at late times of infection indicated that the particles were associated with membranes and the cytoskeleton. although a large amount of cellular and non-structural viral proteins were released at these times, probably by cellular lysis, only virus with an outer layer was found outside the cells, while virus without an outer layer remained associated with the cells, probably with membranes and the cytoskeleton. inhibition of glycosylation by t ...19852999314
detection of rotaviruses by nucleic acid hybridization with cloned dna of simian rotavirus sa11 genes.we developed a dot-blot hybridization assay to detect rotaviral rna sequences in tissue culture or in clinical samples. 32p-labeled cloned cdna probes of the simian rotavirus sa11 specifically detected rotaviral rna sequences and were more sensitive for detecting sa11 than was the commercial enzyme-linked immunosorbent assay rotazyme test. a full-length probe of sa11 gene 6 detected 2.5 x 10(5) sa11 particles or approximately 0.27 ng of purified sa11 dsrna. combined probes from genes 6 and 9 det ...19852993434
anamnestic response in fecal iga antibody production after rotaviral infection of infants. 19852993437
[epidemiology, etiology and laboratory diagnosis of infectious diarrhea diseases in the tropics].diarrhoeal diseases belong to the leading causes of morbidity and mortality in tropical countries, especially in infants and small children. about one billion episodes are estimated for this group of age with 4.6 million fatalities. many causes are discussed to explain the high incidence: bottle feeding of infants, protein malnutrition, unsafe drinking water and unsafe disposal of excrements and sewage, unsufficient consciousness of personal and domestic hygiene, lack of knowledge on the origin ...19853000920
etiologic studies of the 1983 and 1984 outbreaks of epidemic diarrhea in guangxi.studies are reported on two outbreaks of epidemic diarrhea in china involving 19,007 patients. the first outbreak occurred in the northern part of the guangxi autonomous region and extended south from april through september 1983. in june 1984 a second outbreak of 6,570 cases occurred in guanyang, a county in the northern part of guangxi. 125 fecal samples from the two outbreaks were cultured for bacteria; all of the samples except one were negative. rotavirus particles were detected in 44.1% (1 ...19852999028
effect of hypertonic conditions on protein synthesis in ma104 cells infected with human rotavirus.when a high nacl concentration was used to decrease selectively the synthesis of cell proteins, the synthesis of most cellular polypeptides was greatly diminished relative to human rotavirus proteins. thus, in the presence of 150 mm excess nacl, 11 viral polypeptides were clearly identified. however, hypertonic conditions also reduced viral protein synthesis to a different extent with individual proteins. no significant changes in viral protein synthesis occurred during incubation under the hype ...19852999574
characterization of serotypes of human rotavirus strains by solid-phase immune electron microscopy.serotyping of human rotaviruses (hrvs) by neutralization requires the previous adaptation of strains to growth in cell cultures, which is often unsuccessful. by using the solid-phase immune electron microscopy (spiem) technique with protein a and type-specific, cross-adsorbed, polyclonal immune sera, we divided 40 previously typed culture-adapted strains into the same four serotypes distinguished by neutralization, but hrv strains could also be typed directly on stool extracts. of 171 hrv strain ...19852999253
maternal antibody-mediated protection against gastroenteritis due to rotavirus in newborn mice is dependent on both serotype and titer of order to evaluate the role of passively acquired, rotavirus-specific antibodies in protection against diarrhea, we inoculated mouse dams with rotaviruses of various serotypes, and their newborns were orally challenged with a primate rotavirus (simian sa-11). dams were immunized by using a regimen that included repeated inoculations administered either orally or intraperitoneally with adjuvant. the serum antibody response detected in dams by radioimmunoassay and plaque-reduction neutralization ...19852999254
rotazyme assay in neonates without diarrhea. results of screening survey and preliminary analysis of false positive hundred forty-seven stool specimens from 93 infants younger than four months of age in a neonatal intensive care unit were tested for rotavirus by the rotazyme elisa method (abbott laboratories, north chicago, il). none of the infants had diarrhea at the time of the testing. ten of 147 (6.8%) specimens were either low or suspect positive. none had rotavirus by electron microscopy. excluding the suspect positives, which were negative on retesting, the false positive rate was only 6 of 147 (4. ...19853000163
enteric viruses in diarrheic turkey poults.thirty-three intestinal samples from 10-to-21-day-old diarrheic turkey poults were examined for the presence of enteric viruses by electron microscopy. samples originated from 32 flocks in six commercial operations located in six states. mortality in these flocks ranged from 3 to 15%, and birds from recovered flocks varied greatly in size. rotavirus-like agents (rvla) were the most common viruses associated with diarrhea outbreaks in the flocks examined, occurring in five out of six operations. ...19853000337
rotaviruses and immunobiologic failures. 19852999266
pathogens in fecal samples from apparently healthy children. 19853001380
significance of e. coli and rotavirus in infantile diarrhoea. 19853001381
[pathogenesis and immunology of virus-induced neonatal diarrhea]. 19853000346
three-year survey of the epidemiology of rotavirus, enteric adenovirus, and some small spherical viruses including "osaka-agent" associated with infantile diarrhea.studies were made by electron microscopy (em) on the viruses associated with diarrhea of outpatients at a pediatric clinic in osaka prefecture during the three year period from 1980 through 1982. the viruses detected by em by negative staining with phosphotungstic acid (pta) were classified morphologically into 6 groups: rotavirus, adenovirus and four kinds of small spherical viruses, calicivirus, astrovirus, picornavirus/parvovirus (p/p)-like agent and osaka-agent. osaka-agent seems to be a new ...19853000350
a human atypical rotavirus found in an ecuador infant with strain of atypical rotavirus, morphologically indistinguishable from known rotaviruses but lacking the common group antigen of typical rotavirus detectable by an enzyme-linked immunosorbent assay (elisa), was isolated from one of 152 ecuadorian infants with acute rotavirus gastroenteritis. the genome analysis by polyacrylamide gel electrophoresis revealed a unique rna pattern clearly distinct from that of typical rotaviruses.19853000025
rotaviral and coronaviral diarrhea.a number of different viruses can be primary pathogens in the neonatal calf diarrhea complex. by far the most common viruses causing calfhood diarrhea found throughout the world are rotaviruses and coronaviruses. primary infection of newborn calves with either one of these viruses can cause severe intestinal alterations and diarrhea. rotaviruses can produce high-morbidity outbreaks of diarrhea in calves under 10 days of age. morality is variable mainly owing to secondary bacterial infections and ...19853000544
independent segregation of two antigenic specificities (vp3 and vp7) involved in neutralization of rotavirus infectivity.antiserum prepared against the m37 strain of rotavirus, recovered from an asymptomatic newborn infant in venezuela, neutralized two prototype human rotaviruses that define two separate serotypes: serotype 1 (wa) and serotype 4 (st3). thus, the m37 strain is a naturally occurring intertypic rotavirus. analysis of reassortant viruses produced during coinfection in vitro indicated that the observed dual serotype specificity of m37 resulted from sharing a related outer capsid protein, vp3, with the ...19853001716
vaccination against enteric rota and coronaviruses in cattle and pigs: enhancement of lactogenic immunity.passive immunity against enteric viral infections is dependent upon the continual presence in the gut lumen of a protective level of specific antibodies. this article examines methods currently used to enhance the titre and duration of specific antibody in the mammary secretions of cows and pigs, with particular reference to rotavirus and coronavirus infections. in addition, some of the potential problems to be found in attempting to produce vaccines against these viral infections are outlined.19852998111
effect of relative humidity, atmospheric temperature, and suspending medium on the airborne survival of human rotavirus.the wa strain of human rotavirus, grown in ma-104 cells, was suspended either in tryptose phosphate broth or feces from a case of rotaviral diarrhea. it was then aerosolized into a rotating drum using a collison nebulizer. the drum air was sampled using an all-glass impinger containing tryptose phosphate broth as collecting fluid. at 20 +/- 1 degree c, the virus aerosolized from tryptose phosphate broth was found to survive best at 50 +/- 5% relative humidity, where its half-life was 44.2 +/- 6. ...19853000566
rotavirus infections and their prevention by vaccination. 19853002263
development of a sensitive protein a-gold immunoelectron microscopy method for detecting viral antigens in fluid specimens.protein a-colloidal gold immunoelectron microscopy (pag iem) has been employed to specifically detect rotavirus and enterovirus antigen in negatively stained fluid specimens. unlike other iem methods, pag iem can detect not only viral antigen associated with morphologically recognizable particles but also viral antigens of unrecognizable ultrastructure. this rapid and sensitive immunoassay was found to be applicable to virus-infected stool specimens as well as partially purified virus preparatio ...19853001115
use of immunosorbent electron microscopy for detection of rota- and hepatitis a virus in sucrose solutions.immunosorbent electron microscopy was used to demonstrate rotavirus in solutions of varying sucrose concentrations after 18, 42 and 66 h of incubation. about 50% of adsorption of virus particles to the grid was achieved after 18 h incubation and nearly 100% after 42 h when compared to trapping of virus from sucrose free solutions. hepatitis a virus was purified in a 10-30% sucrose gradient and each fraction was examined by immunosorbent electron microscopy, direct electron microscopy, immune ele ...19853001117
detection of rotavirus by latex agglutination. 19853001154
rotavirus & bacterial enteropathogens in acute diarrhoeas of young children in bangalore. 19852997035
rna genome electrophoretic analysis of rotavirus from feces of epidemic adult diarrhoea occurring in shandong province, china. 19852999216
clinical and biochemical aspects of bovine rotavirus infection in iraq. 19852999218
sugar intolerance complicating acute gastroenteritis.sugar intolerance occurred in 31 of 200 children admitted to hospital with acute gastroenteritis. in 28 this was transient and settled rapidly, but in the remaining three it indicated a more serious and persistent problem. the most important predisposing factor was viral infection, in particular with rotavirus. the current regimen for the management of sugar intolerance complicating acute gastroenteritis at this hospital is outlined.19854062353
rotavirus infections among the staff of a general paediatric department. 19854024935
bovine cryptosporidiosis in denmark. 2. cryptosporidia associated with neonatal calf recent years cryptosporidia have often been identified in diarrheic calves of under one month old, either as the sole pathogenic agent or in combination with other diarrhea-causing infectious agents. in 203 cases of bovine cryptosporidiosis, recorded over a period of two years, cryptosporidia were present in such great numbers that they would seem to be of causal significance. the calves were from three days to five weeks old. in 111 cases (55%) a monoinfection with cryptosporidia occurred, w ...19853991354
acute diarrhoeal disease in children under 7 years of age in a peri-urban slum of santiago, chile.a group of 168 families who lived in a peri-urban slum in santiago were surveyed for 9 months. all of them had a child under 7 years of age. medical activities and data collection were carried out at a field station and by means of twice-weekly visits to each home, at which time cases of diarrhoea were recorded and investigated. faecal samples for bacteriological, parasitological and rotavirus studies were obtained during each episode. the characteristics of clinical course, hygienic practices i ...19854067299
prevention of rotavirus infection by oral administration of cow colostrum containing antihumanrotavirus antibody.after immunizing 8-month pregnant holstein cows with human rotavirus, wa strain, cow colostrum containing neutralizing antibody to human rotavirus, designated as rota colostrum, was obtained. after randomly grouping 13 infants from a single orphanage, 6 infants received 20 ml of rota colostrum every morning and 7 control infants received 20 ml of market milk. one month later, rotavirus associated diarrhea was observed in 6 of the 7 infants given milk and 1 out of the 6 infants given rota colostr ...19854069083
[respiratory and enteric rotavirus infections in a pediatric unit]. 19854069693
[prospective study of rotavirus infection in a maternity unit. demonstration of a nosocomial infection].eighty-eight children born at the maternity hospital in saint-germain-en-laye between may 24 and june 7, 1983 were followed clinically, with a special supervision concerning stools, weight curves and the way of feeding. stool samplings looking for rotavirus were performed in all the children and their mothers, at the 3rd and 6th days of life. no mother was found with rotavirus infection. in neonates, rotavirus excretion was significantly related to a slow down in weight curves and the occurrence ...19854074098
rapid coagglutination test for detection of rotaviruses in turkeys.avian rotaviruses present in fecal samples were readily detected using a staphylococcal protein-a coagglutination test on a white porcelain plate. staphylococci, which produced large amounts of protein-a, were coated with rabbit anti-avian rotavirus serum. the antibody-coated staphylococci were agglutinated specifically by rotavirus present in the fecal sample. the macroscopic agglutination reaction occurred within a few minutes. a total of 40 fecal samples were tested by the coagglutination tes ...19854074236
experimental rotavirus diarrhoea in colostrum-deprived newborn calves: assay of treatment by administration of bacterially produced human interferon (hu-ifn alpha 2).seven colostrum-deprived newborn calves were orally inoculated within 24 hours after birth with bovine rotavirus. three of them were intramuscularly injected with bacterially produced human interferon (hu-ifn alpha 2). the four control animals presented a severe diarrhoea for at least 48 hours, while only one of the treated calves suffered from a transient diarrhoea for a few hours. hu-ifn alpha 2 seems therefore able to control rotavirus diarrhoea in newborn calves, although it did not inhibit ...19854062197
aetiological least 25 etiologic agents have been implicated in acute diarrhea. in children, the most common are the rotaviruses, enterotoxigenic escherichia coli, campylobacter jejuni, salmonallae, and shigellae. for example, an attempt was made to isolate enteropathogenic micro-organisms in children under 2 years of age hospitalized in ethiopia with severe or moderate dehydration. a causal agent was identified in 70% of the 175 cases analyzed. 49% of these cases involved rotavirus infections, 19% inc ...198512314104
the gambia and bangladesh: the seasons and diarrhoea.climactic factors in the gambia and bangladesh have an important impact on the incidence of diarrheal disease. both countries share some common characteristics in climate, including a cool dry winter of 3 months followed by a hot dry spring and hot wet summers of 5-7 months in length. the main difference is in the amount of rainfall. the gambia may have 20-30 inches of rain each year; bangladesh usually has up to 4-5 times this amount. in the gambia, drought is a recurring problem; floods is ...198612315285
pathogenesis of rotavirus infection in turkey poults.the pathogenesis of rotavirus infection was examined after experimental infection of conventional and specific-pathogen-free (spf) turkey poults. in six experiments birds were exposed to turkey rotavirus isolates tu-1 or tu-2 or the chicken isolate ch-1 at 7, 10 or 42 days of age. poults were examined between 1 and 24 days post-infection (dpi) for diarrhoea, gross and histopathologic lesions, virus excretion in the intestinal tract, viral antigen in intestinal epithelial cells, and the developme ...198618766543
electrophoretic variation of avian rotavirus rna in polyacrylamide gels.five different sorts of rna profiles (electropherogroups 1 to 5) were recognised when avian rotavirus rnas were electrophoresed in polyacrylamide gels. these could be distinguished from the rnas of mammalian group a, b and c rotaviruses. viruses belonging to electropherogroups 1, 2/3 and 4 were detected in chickens and viruses belonging to electropherogroups 1, 2, 3 and 5 were detected in turkeys. a minimum of three electropherogroups was recognised in each of four longitudinal surveys of broile ...198618766513
cytopathic avian rotavirus isolated from duck faeces in chicken kidney cell cultures.five cytopathic viruses morphologically resembling rotaviruses were isolated from duck faeces in chicken kidney cell cultures using conventional methods. one of the isolates, designated f-29 strain, was identified as an avian rotavirus from the following: ribonucleic acid in the viral core, virus growth in the cytoplasm, resistance to chloroform, acid and heating, partial stabilisation to molar magnesium chloride and rotavirus-like morphology by electron microscopy. by immunofluorescence, f-29 s ...198618766573
molecular epidemiology of human rotavirus.the epidemiology of human rotavirus (hrv) in north-east scotland was investigated between 1982 and 1984. during this period 708 hrv infections were recorded. the majority (83%) of the infections were in children less than 5 years of age although some were recorded in adults. the peak incidence occurred in the winter months although a high number of hrv infections was reported during the summer of 1983. a total of 840 faecal specimens containing rotavirus were screened for hrv genome rna by polya ...19863701041
analysis of reassortment of genome segments in mice mixedly infected with rotaviruses sa11 and cd-1 mice born to seronegative dams were orally inoculated with a mixture of wild-type simian rotavirus sa11 and wild-type rhesus rotavirus rrv. at various times postinfection, progeny clones were randomly isolated from intestinal homogenates by limiting dilution. analysis of genome rnas by polyacrylamide gel electrophoresis was used to identify and genotype reassortant progeny. reassortment of genome segments was observed in 252 of 662 (38%) clones analyzed from in vivo mixed infe ...19863001336
protective effect of naturally acquired homotypic and heterotypic rotavirus assess serotype specificity of immune resistance to rotavirus gastroenteritis, the relation between pre-existing neutralising antibodies to homotypic and heterotypic rotaviruses and protection against infection or clinical illness was investigated. the subjects were 44 orphans exposed once or twice to consecutive outbreaks of gastroenteritis due to type 3 rotavirus in an orphanage in sapporo. sera were collected throughout these outbreaks and the serum levels of neutralising antibodies agains ...19862874413
failure of live, attenuated oral rotavirus vaccine. 19862873370
rotavirus-associated diarrhea in a commercial epizootic of diarrheal disease occurred in a commercial specific-pathogen-free rabbitry, and was characterized by sudden onset, rapid spread, and high morbidity and mortality among sucklings. affected rabbits rapidly became dehydrated and most died within two days of the onset of diarrhea. eight of these rabbits were necropsied. five had blunted and fused small intestinal villi with attenuated villous enterocytes. a rotavirus was isolated from four rabbits, and five survivors of affected litt ...19862939288
the detection of enteropathogens in acute diarrhea in a family cohort population in rural 8 villages of rural northeastern egypt, a 2-year study of the etiologic agents associated with episodes of diarrhea was carried out. stool specimens (3,243) from 3,513 episodes of diarrhea were processed for enteropathogens. the most commonly identified agents in the group with diarrhea were giardia lamblia (44%), heat stable enterotoxin (st)-producing enterotoxigenic escherichia coli (etec) (15%), heat labile toxin (lt)-producing etec (12%), enteropathogenic e. coli (epec) (4%), rotavirus (3 ...19862876656
prevalence of various enteropathogens in the feces of diarrheic and healthy calves.the presence of various enteropathogens was examined in the feces of homebred dairy calves reared in a restricted geographical area of france (north west of county of indre-et-loire) during winter 1983-1984. two distinct surveys were carried on: a case-control study including 32 diarrheic calves and 21 healthy calves bred in 53 different farms; and a separate study on nine diarrheic calves in another farm. the following infectious agents were looked for, by specific methods of detection: escheri ...19862877609
self-contained enzymic membrane immunoassay for detection of rotavirus antigen in clinical samples.a self-contained enzymic membrane immunoassay (scemia) system has been developed for the detection of viral antigens in clinical samples. the assay system makes use of antiviral antibodies bound to a nylon membrane, a flow-through washing procedure, and a clearly visible endpoint of the enzymic reaction. a scemia system with antibodies against rotavirus detected rotavirus antigen, within 15 min, in all faecal samples from children with gastroenteritis that were positive for antigen in a standard ...19862878176
prevalence of k88, k99, and 987p pili of escherichia coli in neonatal pigs with enteric hundred nineteen live neonatal pigs with diarrhea less than or equal to 2 weeks old were euthanatized, and frozen sections of their ilea were submitted to an indirect fluorescent antibody technique to identify k88, k99, and 987p pili (also referred to as f-4, f-5, and f-6 pili, respectively) in escherichia coli. ten-centimeter ileal sections were used to determine numbers of lactose-fermenting bacteria. of 52 pigs in which e coli pili were found, 14 had k88 (27%), 23 had k99 (44%), 13 had 98 ...19862878634
rotavirus-like infection associated with diarrhea in okapi. 19862851576
[incidence of rotavirus in the menino jesus pediatric hospital (sp): elisa--a practical and efficient method for its detection].seventy infants and children with age from 2 months to 4 years old were admitted to "hospital infantil menino jesus", in são paulo, brazil, in the period from march 1983 to june 1984 with acute diarrhea. feces from these patients were analysed in the search of rotavirus and enteropathogenic bacteria (shigella, salmonella and e. coli), for the purpose of defining the role of rotavirus as an agent of acute diarrhea in infants and young children in this country. elisa (enzyme-linked immuno sorbent ...19862831862
derivation of a monoclonal antibody against the group specific antigen of rotaviruses and its use in a diagnostic enzymatic immunoassay. 19862827408
seroepidemiology of rotavirus in singapore. 19862822792
[ultrastructure of rotavirus].the human rotavirus is an icosadeltahedral structure, with a characteristic pattern of a triangulation number (t) of 13 with a skew symmetry. the inner layer of the rotavirus has 132 morphological units, with 260 structural units of trimetric structure. it is possible that the outer layer is a smooth geodesic shell.19862823323
synthesis of simian rotavirus sa11 double-stranded rna in a cell-free system.a cell-free system was developed to study the replication of simian rotavirus sa11. the components of the system included (i) subviral particles prepared from infected cells to template the synthesis of viral rna and (ii) an mrna-dependent rabbit reticulocyte lysate to support protein synthesis. based upon nuclease-sensitivity, approximately 20% of the rna made in vitro was double-stranded (dsrna) and 80% single-stranded (ssrna). electrophoretic analysis of the rna products on polyacrylamide and ...19862437720
genetic reassortment between two human rotaviruses having different serotype and subgroup specificities.two reassortant viruses were selected from a mixed infection of ma104 cells with human rotavirus strains wa (serotype 1-subgroup ii) and hn126 (serotype 2-subgroup i). antigenic characterization and genotype analysis by polyacrylamide gel electrophoresis revealed that they were reassortants with novel antigenic compositions, i.e. serotype 1-subgroup i (c116) and serotype 2-subgroup ii (c15). furthermore one of them, c15, was considered to have a mosaic antigenicity defined by two serotype-specif ...19862426394
rna-binding proteins of bovine rotavirus.two major bovine rotavirus proteins have rna-binding activity as shown by an rna overlay-protein blot assay. of the six proteins in purified virions, only one showed rna-binding activity. this 92,000-molecular-weight (92k) protein was present in both single- and double-shelled particles. its rna-binding activity was blocked by preincubation with monospecific antibody to vp2. thus, the 92k rna-binding protein in rotavirus virions is vp2, the second most abundant protein in single-shelled particle ...19862422396
location of the major antigenic sites involved in rotavirus serotype-specific neutralization.antigenic sites on the rotavirus major outershell glycoprotein were identified by using mutant viruses selected for resistance to neutralizing (serotype-specific) monoclonal antibodies. the glycoprotein genes from these mutants were sequenced to determine the position and nature of the resultant amino acid substitutions in the protein. three regions (a, b, and c) were identified (amino acids 87-96, 145-150, and 211-223, respectively), of which region c appears to be the most important. a mutatio ...19862422651
protective effect of orally administered immunoglobulins against experimental calf diarrhea. 19862423734
passive protection against rotavirus-induced diarrhea by monoclonal antibodies to surface proteins vp3 and vp7.monoclonal antibodies directed against two rotavirus surface proteins (vp3 and vp7) as well as a rotavirus inner capsid protein (vp6) were tested for their ability to protect suckling mice against virulent rotavirus challenge. monoclonal antibodies to two distinct epitopes of vp7 of simian rotavirus strain rrv neutralized rrv in vitro and passively protected suckling mice against rrv challenge. a monoclonal antibody directed against vp3 of porcine rotavirus strain osu neutralized three distinct ...19862422398
[immunotherapy in virus diseases].the number of effective anti-viral agents is still limited. therefore immunotherapy remains of importance in the treatment of viral infections. immunoglobulins and interferons as well as a variety of immune stimulators or immune modulators are available. the efficacy of such therapy strongly depends on the kind of the virus causing the infection as well as the time of initiation of the treatment. the prophylactic and therapeutic effects of immunoglobulin preparations are well documented, whereas ...19862422548
derivation of neutralizing monoclonal antibodies to human rotaviruses and evidence that an immunodominant neutralization site is shared between serotypes 1 and 3.neutralizing monoclonal antibodies were derived to human rotaviruses rv-4 (serotype 1), rv-5 (serotype 2), and st-3 (serotype 4). by enzyme immunoassay and fluorescent focus neutralization, eight of the antibodies appeared to be specific for the immunizing serotype, and so have potential as reagents for rotavirus serotyping by enzyme immunoassay. seven of these were shown by western blotting, enzyme immunoassay for antibody additivity, and reaction with rotavirus reassortants, to be directed aga ...19862429439
prevention of calf diarrhea with an immunoglobulin diet in beef herds. 19862431184
antigenic mapping of the surface proteins of rhesus rotavirus.monoclonal antibodies have been produced and used to map the functional topography of the surface proteins of rhesus rotavirus (rrv) that mediate viral neutralization. ten monoclonal antibodies directed to vp7 were studied in neutralization assays and competitive binding studies. a large neutralization domain with several interrelated epitopes on vp7 was apparent. twelve monoclonal antibodies directed to vp3 were used in similar studies and delineated at least 2 distinct neutralization domains o ...19862431540
use of microplate cell culture and enzyme immunoassay in titration of serum neutralizing antibody against hochi strain of serotype 4 human rotavirus.the tube neutralization test read by enzyme immunoassay developed by wyatt et al. (1983) for serotype determination of human rotavirus was modified so as to use stationary cultures of ma104 cells in a microtiter plate instead of roller tube cultures. sera obtained from different age groups were titrated for neutralizing antibody against serotype 4 human rotavirus hochi strain by this test and the results were compared with those obtained by the plaque neutralization test. there was a good correl ...19863035256
[etiological significance of rotaviruses in acute intestinal diseases in children].the paper presents the results of virological, serological, and epidemiological examinations of patients with acute enteric infections of obscure etiology carried out for detection of rotavirus gastroenteritides. rotaviruses have been found to play a certain role in generating acute gastroenteritides in infants under 3 years, mostly among infants, of 1-2 years, the least among those of 0-6 months of age. adult family members were the source of infection for the children. the season of infection ...19863030001
[immunoenzyme analysis in the diagnosis of human rotavirus gastroenteritis (methodological aspects)]. 19863030005
[action of various disinfectants on 7 envelope-free viruses].the activity of 15 disinfectants on seven nude viruses belonging to groups significant in human and veterinary medicine and in the bio-industry was investigated by a dilution technique. these trials, performed organic matters being either present or not revealed that current products such as 0.8% sodium hydroxyde and 1 degree chlorine water were the most active. according to their compositions, the chemicals varied greatly in their efficacy.19863030183
[isolation of human rotaviruses in cultures of rhesus monkey kidney cells]. 19863028583
diagnostic significance of specific iga coproconversion in rotavirus infection. 19863028811
comparison of reverse passive haemagglutination assay & solid phase agglutination of coated erythrocytes with elisa for rotavirus antigen detection. 19863028947
[technics used in viral genetics as an alternative means of diagnosing viral diseases]. 19863031336
response to rit 4237 oral rotavirus vaccine in breast-fed and formula-fed infants.twenty-six full-term newborns (15 males and 11 females) were followed-up from birth to 5 months of age. during the first month of life, all of them were breast-fed. thereafter those infants whose mothers produced enough milk continued breast-feeding (n = 16) while the remaining (n = 10) changed to an adapted milk formula supplying approximately 2 g/kg/day of protein and 100 kcal/kg/day. at 4 months of life, all infants were vaccinated with one oral dose of rit 4237 rotavirus vaccine of bovine or ...19863019954
chemical disinfection of human rotavirus-contaminated inanimate surfaces.fomites may play a role in the transmission of rotavirus infections, and in view of this, 27 disinfectants were evaluated for their ability to inactivate human rotavirus (hrv) on contaminated non-porous inanimate surfaces. disks of stainless steel, glass and two types of plastics were contaminated with about 10(7) plaque-forming units of hrv suspended in faecal matter. the inoculum was allowed to dry and an equal volume of the product under test was applied to the contaminated surface. after con ...19863016082
further study of the epidemiology of rotavirus infection in tokyo. 19863015626
conservation of the fourth gene among rotaviruses recovered from asymptomatic newborn infants and its possible role in attenuation.rna-rna hybridization was performed to assess the extent of genetic relatedness among human rotaviruses isolated from children with gastroenteritis and from asymptomatic newborn infants. 32p-labeled single-stranded rnas produced by in vitro transcription from viral cores of the different strains tested were used as probes in two different hybridization assays: undenatured genomic rnas were resolved by polyacrylamide gel electrophoresis, denatured in situ, electrophoretically transferred to diazo ...19863023685
primary isolation and identification of avian rotaviruses from turkeys exhibiting signs of clinical enteritis in a continuous ma 104 cell line.avian rotaviruses were isolated from turkeys with enteritis using ma 104 cell line. ma 104 cells were suitable for primary isolation and propagation of avian rotaviruses. trypsin appeared essential for the enhancement of infectivity and the occurrence of cytopathic effect (cpe). serum neutralization (sn), electron microscopy (em), and analysis of genomic rna were done to identify and confirm the identity of rotaviruses. electrophoretic migration patterns of genomic rna from avian rotaviruses wer ...19863021099
[a study on the rotavirus antibody in the normal human sera using elisa]. 19863024842
comparison of four commercial assays for detection of rotavirus in childhood gastroenteritis. 19863023075
antibody against adult diarrhoea rotavirus among healthy adult population in china.this paper deals with a method of using unknown serum as the first antibody to coat wells of the microtiter plate directly in application of the sandwich elisa method with double antibodies for the assay of the antibody against adult diarrhoea rotavirus. this method was used for assay of the antibody against adult rotavirus in 1,380 sera of healthy adults obtained from some provinces and cities of china, of which 141 showed a positive result with a total positive rate of 10.2%. the infection rat ...19863021802
single gene substitution rotavirus reassortants containing the major neutralization protein (vp7) of human rotavirus serotype 4.a series of reassortants was isolated from coinfection of cell cultures with wild-type bovine rotavirus (uk strain [serotype 6]) or rhesus rotavirus (strain mmu18006 [serotype 3]) and a tissue culture-adapted human rotavirus strain, st3 (serotype 4). monospecific antiserum or a set of monoclonal antibodies to the major outer capsid neutralization glycoprotein, vp7, of the animal rotavirus parent was used to select for reassortants with human rotavirus serotype 4 neutralization specificity. the m ...19863021814
detection of enteropathogens in fatal and potentially fatal diarrhea in cairo, egypt.a 1-year study of the etiology of acute diarrhea complicated by severe (10%) dehydration, active bleeding, shock and cardiovascular collapse, pneumonia, acute renal failure, or seizures in infants under 18 months of age was performed in cairo, egypt. of 145 infants, 19 (13%) died or left the hospital moribund; the remaining 126 patients were classified as having potentially fatal illness. a variety of enteropathogens were identified with approximately equal frequency in the fatal and nonfatal co ...19863023441
safety and immunogenicity of live attenuated rhesus monkey rotavirus vaccine. 19863023497
human rotavirus studies in volunteers: determination of infectious dose and serological response to unpassaged, safety-tested strain (cjn) of human rotavirus from a stool specimen of a hospitalized child was administered orally to 62 adult volunteers for determination of the dose required to produce infection with or without illness. subjects ingested doses ranging from 9 x 10(-3) to 9 x 10(4) focus-forming units in buffered salt solution after consumption of 50 ml of 4% nahco3. the amount of virus in the inoculum required to cause infection (shedding of virus, seroconversion, or both) in s ...19863021869
conservation of amino acid sequence of vp8 and cleavage region of 84-kda outer capsid protein among rotaviruses recovered from asymptomatic neonatal infection.within the past few years, rotavirus strains were recovered from four discrete prolonged outbreaks of infection in newborn nurseries in which affected infants failed to develop significant symptoms. the virus strains recovered from each outbreak belonged to a different human rotavirus serotype and thus each of the four human rotavirus serotypes was associated with asymptomatic infection of neonates. marked conservation of sequence was observed among the fourth genes of the nursery rotavirus stra ...19863018754
epidemiology of rotavirus gastroenteritis. 19863021890
reassortant rotaviruses containing structural proteins vp3 and vp7 from different parents induce antibodies protective against each parental serotype.genetic studies of reassortant rotaviruses have demonstrated that gene segments 4 and 9 each segregate with the serotype-specific neutralization phenotype in vitro. reassortant rotaviruses derived by coinfection of ma-104 cells with the simian strain sa11 and the antigenically distinct bovine strain ncdv were used to determine which viral genes coded for proteins which induced a protective immune response in vivo. in addition, reassortant rotaviruses containing only the gene segment 4 or 9 prote ...19863021983
a scanning and transmission electron microscopic study of rotavirus-induced intestinal lesions in neonatal gnotobiotic dogs.neonatal gnotobiotic dogs orally inoculated with canine rotavirus had ultrastructural changes limited to the jejunal and ileal regions of the small intestine. early scanning electron microscopic findings consisted of swollen villus epithelial cells, denuded foci on intestinal villi, and slight to moderate villus atrophy. later changes were slight villus atrophy with no denuded intestinal villi. transmission electron microscopic changes in villus epithelial cells from 12 to 48 hours post-inoculat ...19863018981
experimental infection of specific-pathogen-free chickens with avian specific-pathogen-free chickens were infected with rotaviruses isolated from chickens, turkeys, and pheasants. chicks infected at 1 or 7 days of age remained largely free of infection: virus could be isolated from a few chicks of these ages, but virus antigen could not be detected in the epithelial cells of the intestinal villi. treating the virus with trypsin before inoculation did not markedly enhance virus infectivity. the same batches of virus successfully infected chickens ...19863021101
detection of transmissible gastroenteritis coronavirus antigens by a sandwich enzyme-linked immunosorbent assay technique.a new sandwich enzyme-linked immunosorbent assay, using monoclonal and polyclonal antibodies, was developed to detect transmissible gastroenteritis virus antigens from cell culture and from intestinal wash or feces obtained from experimentally infected pigs. this technique was shown to be suitable for the detection of virulent field strain unadapted to cell culture. cross reactions had not been observed with other enteric pathogens, rotavirus, porcine epizootic diarrhea virus, and escherichia co ...19863024536
childhood gastroenteritis: a population study.a prospective study of gastroenteritis based on a population was carried out for 12 months on over 7000 children in general practice. the incidence of gastroenteritis was highest in the first year (127.7 children affected per 1000) and second year (90.8) of life, and gastroenteritis was rare after six years of age. children from urban areas had gastroenteritis more commonly than children from semirural areas. a potential pathogen was isolated from half of the specimens: 78% were viruses, and rot ...19863019460
epidemiologic and genomic study of rotavirus strains infecting young children and calves in the same rural environment.the epidemiological profile of rotavirus infection in young calves and young children form the same rural environment in france was studied during autumn-winter 1983-1984. viruses were isolated from calves on each farm and were identified by their electropherotype for 8 out of the 9 farms under study. among 13 children regularly checked on 9 farms for 25 weeks, 7 were found to be rotavirus positive. nevertheless, no transfer of virus between child and bovine was documented by the electropherotyp ...19863021523
[frequency of the rotavirus antigen, detected by the elisa method, in the feces of patients with infantile enteritis]. 19863020668
detection of group rotavirus antigen with romanian elisa kits in the acute gastroenteritis of the infant and young child. 19863020670
electropherotyping of human rotaviruses: an epidemiological survey of rotavirus infections in electrophoretic analysis of rotavirus rna segments was carried out on 522 faecal specimens, obtained from children hospitalized in sicily in the period 1981/85. one hundred and one viral isolates could be characterized with respect to the electrophoretic pattern of their genomic rnas. this analysis revealed that in 1981/82 different electropherotypes cocirculated in the infant population. in 1983 one of the patterns became prevalent; in 1984/85 only one electropherotype was detected, both in ...19863021522
the effect of trypsin on the growth and infectivity of human rotavirus.the effect of trypsin on the infectivity of human rotavirus (hrv) was examined using hrv strains that are cultivable only when treated with trypsin. their infectivity in cell culture systems was enhanced in relation to enzyme concentration. the primary effect of trypsin appeared to be on the virus. trypsin untreated hrv virions were capable of being adsorbed on the cell (ma104) but not of passing into it without trypsin. for multiple cycles of replication the presence of trypsin in the maintenan ...19863022422
detection of transmissible gastroenteritis virus in feces from pigs by reversed passive hemagglutination.a reversed passive hemagglutination (rpha) method was developed for the detection of transmissible gastroenteritis (tge) virus in the fecal specimens from pigs. ovine erythrocytes fixed with glutaraldehyde and treated with tannic acid were coated with anti-tge virus swine antibodies, which were purified by affinity chromatographic technique linked with purified tge virus. the rpha test was done by the microtiter method. erythrocytes coated with purified specific antibodies were agglutinated by t ...19863022621
homotypic and heterotypic antibodies for prevention of experimental rotavirus gastroenteritis.we investigated the abilities of homologous and heterologous antibody preparations to neutralize murine rotavirus in a mouse model of rotavirus infection. we found that incubation of virus with murine sera obtained from animals experimentally infected with the homologous epizootic diarrhea of infant mice strain of rotavirus resulted in inability of the virus to cause symptomatic disease in infant mice. sera from nonimmune mice and mice infected or immunized with a heterotypic strain of rotavirus ...19863023437
heterologous protection against rotavirus-induced disease in gnotobiotic piglets.administration per os of 2 x 10(6) fluorescent cell-forming units of a human serotype 3 rotavirus (rv-3) protected all of nine gnotobiotic piglets against severe diarrheal disease when they were challenged 10 to 14 days later with 8 x 10(3) fluorescent cell-forming units of virulent wild-type porcine rotavirus (at/76). the porcine virus was similar antigenically to porcine prototype strain osu, previously described as antigenically distinct from all four recognized human serotypes. administratio ...19863023436
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