Publications

[intestinal rotavirus infections (hrv - human rotavirus)].		1982	6302630
[infectious diarrhea of viral and bacterial etiology].		1982	6302816
comparison of an enzyme immunoassay with electron microscopic procedures for detecting rotavirus.	the sensitivity and specificity of an enzyme immunoassay (rotazyme), an ongrid immunoelectron microscopy procedure, and conventional negative stain electron microscopic techniques were compared. by using partially purified human rotavirus and simian rotavirus (sa-11) of known particle concentration, the enzyme immunoassay was essentially equivalent to the immunoelectron microscopic procedure and significantly more sensitive than conventional electron microscopic techniques. the level of sensitiv ...	1982	6284793
detection of human rotavirus by reversed passive hemagglutination (rpha) using antibody against a cultivable human rotavirus as compared with electron microscopy (em) and enzyme-linked immunosorbent assay (elisa).		1982	6294488
new agents in diarrhea.		1982	6294627
new opportunities for development of safe, effective live virus vaccines.	effective vaccines are not available for most viral diseases. this situation may soon change when the full force of contemporary molecular biology is applied to immunoprophylaxis. in certain viral diseases, particularly those affecting the respiratory and gastrointestinal tracts, live attenuated vaccines are needed to confer effective protection. until now the major obstacle to success has been genetic instability. it may be possible to construct stable, satisfactorily attenuated mutants by clon ...	1982	6295011
electrophoretic analysis of rna segments of human rotaviruses cultivated in cell culture.	human rotaviruses (hrvs) derived from stools were cultivated to high titres in an established cell line (ma-104) using the rotary culture system. analysis of the 11 double-stranded rna segments of the culture-adapted hrvs was carried out by polyacrylamide gel electrophoresis. tissue culture-adapted hrvs, uncloned or cloned, had the same rna gel patterns as those of the original hrvs from the individual stool specimens. the migrations of the rna segments from the culture-adapted hrvs were markedl ...	1982	6284865
antibodies to gastroenteritis viruses in cystic fibrosis patients.	infectivity of certain enteric viruses including rotavirus is profoundly affected by proteolytic enzymes. to test whether cystic fibrosis patients, possessing chronically decreased levels of pancreatic enzymes, show altered susceptibility to gastroenteritis viruses, we examined sera from patients and controls for antibodies to two major pathogens. in cystic fibrosis patients, normal rotavirus antibody levels were found and norwalk virus antibody prevalence was unchanged.	1982	6284870
rotavirus: a cause of nosocomial infection in the nursery.		1982	6284903
rotavirus and hemolytic enteropathogenic escherichia coli in weanling diarrhea of pigs.	since the turn of the century, escherichia coli has been implicated in the etiology of weanling diarrhea (colibacillosis). however, rotavirus--a virus that destroys enterocytes--has been shown recently to be causally associated with weanling diarrhea of pigs. the role of both rotavirus and hemolytic enteropathogenic e. coli in weanling diarrhea was assessed in this study. pigs from a closed herd were farrowed and weaned by two markedly different systems: an "intensive care sanitary" system and a ...	1982	6296193
detection of a rotavirus-like agent associated with diarrhea in an infant.	a rotavirus-like agent was detected in the feces of a child with diarrhea. although morphologically indistinguishable from rotavirus, the agent was serologically distinct, and electrophoresis of its nucleic acid also showed that it was dissimilar.	1982	6296194
isolation of human rotavirus subgroups 1 and 2 in cell culture.	one strain of human rotavirus subgroup 1 (kun) and one strain of subgroup 2 (mo) were isolated with the ma104 cell line, a fetal rhesus monkey kidney cell line. their subgroup specificities and rna patterns were identical to those of rotaviruses present in stools before cultivation. distinct cytopathic effects consisting of obscure cell boundaries, cell fusion, cell rounding, cell detachment, and lytic foci were recognized at passage 3 of mo and passage 6 of kun. no differences in cytopathic cha ...	1982	6296195
rotavirus infection in nigerian infants and young children with gastroenteritis.	in a study conducted over a 5-month period (september 1979 to january 1980), rotaviruses were detected by counterimmunoelectrophoresis in the feces of 16 (13.8%) of 116 infants and young children between 1 and 48 months old with acute, sporadic gastroenteritis, at ife, nigeria. the highest frequency of rotavirus infection was found in th 7- to 12-month age-group (18.9%). males constituted 61% of the sample and excreted rotavirus at a significantly higher rate than did females (p less than 0.01). ...	1982	6280506
electron microscopy of the intestine of gnotobiotic piglets infected with porcine rotavirus.		1982	6296207
enteric viruses of nonhuman primates.	the phylogenetic relationship of nonhuman primates to man implies that many of these animals could serve as surrogates for studies of diseases of man. many nonhuman primate species are susceptible not only to viruses of human origin but also to nonhuman primate viruses that are counterparts of viruses of man. all monkeys and great apes do not respond similarly to an antigenic stimulus. some agents are highly pathogenic for one species and completely innocuous for another. for example, poliovirus ...	1982	6293149
serotyping and subgrouping of rotavirus strains by the elisa test.	a method is described for the serotyping and subgrouping of rotaviruses by enzyme-linked immuno-sorbent assay (elisa). for the isolates tested "blind" under code in parallel the serotyping results obtained by elisa and serum neutralization of fluorescent focus formation were the same. after absorption of the typing antisera with purified heterotypic rotavirus, up to 128-fold differences in titres between isolates were observed in the elisa test. the results of serotyping and subgrouping, by elis ...	1982	6293413
[detection of rotavirus in hospitalized and ambulatory infants with acute diarrhea in santiago de chile].		1982	6284181
rotaviral antibodies in cow's milk.	in the past, it has been reported that neonatal diets made from unheated cow's milk were superior to those made from heated cow's milk. it was observed that piglets were equally protected from rotaviral diarrhea when they were fed diets made from either unheated milk that came from a cow immunized against porcine rotavirus or from a cow that was not immunized. because of this observation, we examined four pools of "normal" cows' colostrum and 58 samples of "normal" cow's milk for the presence of ...	1982	6293690
molecular biology of rotaviruses. iv. molecular cloning of the bovine rotavirus genome.	a new cloning strategy has been developed for cloning the genomes of double-stranded rna viruses by using bovine rotavirus as a test system. the major modification adopted was the use of denatured polyadenylated double-stranded rna as the template for reverse transcriptase. this allowed the two complementary strands of cdna to be synthesized in a single reaction and removed the need for s1 nuclease digestion to remove the 5' hairpin structure normally generated in cdna synthesis.	1982	6294322
new prospects for control of virus diarrhoea in children.		1982	6283077
isolation of lapine rotavirus in cell cultures. brief report.	two cytopathic rotavirus strains were isolated in ma 104 cells from diarrheal rabbits. the isolates showed marked cross reactions with strain lincoln of bovine rotavirus in immunofluorescence, but no cross reaction in neutralization.	1982	6285861
[efficacy testing of inactivated rotavirus vaccine in a dairy cattle herd].		1982	6291485
isolation and identification of swine rotavirus in taiwan.	large numbers of viral particles resembling rotavirus were detected with negative stained electron microscopy in bacteria free fecal filtrate obtained from 10-day old diarrheal suckling piglets of a conventional pig farm in taiwan. the clinical signs of vomiting and diarrhea were reproduced in colostrum deprived piglets artificially infected with the fecal filtrates. rotavirus particles persisted in the fecal samples after two in vivo serial passages, and was not seen in the uninfected control a ...	1982	6291876
gene protein products of sa11 simian rotavirus genome.	when ma104 cells were infected with sa11 rotavirus, 12 protein classes, absent in mock-infected cells, could be distinguished by polyacrylamide gel electrophoresis. at least two of these proteins were glycosylated, and their synthesis could be blocked with tunicamycin. the oligosaccharides of both glycoproteins were cleaved by endo-beta-n-acetylglucosaminidase h, suggesting that they were residues of the "high-mannose" type. of the 12 viral polypeptides observed in infected cells, 1 was probably ...	1982	6283128
molecular biology of rotaviruses. iii. isolation and characterization of temperature-sensitive mutants of bovine rotavirus.	twenty-six temperature-sensitive (ts) mutants of united kingdom tissue culture-adapted bovine rotavirus were isolated and characterized. fourteen of these mutants were determined to be ts both by efficiency of plating and by virus yield at the nonpermissive temperature of 39.5 degrees c as compared with that at the permissive temperature of 32 degrees c. the remaining mutants were only ts by the criterion of efficiency of plating. high-frequency recombination (gene reassortment) was observed whe ...	1982	6283176
age-dependent rotavirus-enterocyte interactions.		1982	6283556
identification of a neutralization-specific antigen of a calf rotavirus.	monospecific polyclonal antisera were raised in guinea-pigs against the calf rotavirus polypeptides vp1, vp2, vp3 + 4, vp4.2, vp6, vp7.1, vp7.2 and vp10. all of the antisera gave a similar pattern of cytoplasmic immunofluorescence in rotavirus-infected cells, but spots of fluorescence of varying intensity with different sera were seen over the nucleus. immune precipitation, using staphylococcus aureus to collect immune complexes, showed that vp2 was precipitated by antiserum to vp2 (alpha-vp2) a ...	1982	6292353
in vitro transcription of two human rotaviruses.	the rna polymerase activities of a cultivatable (wa) and a noncultivatable (ds-1) strain of human rotavirus were studied. under optimal conditions, transcription of all of their rna segments occurred, as evidenced by the hybridization of labeled transcripts to genomic rna. cross-hybridization between the two viruses showed that none of their 11 genes were completely homologous. the transcription products could be translated in vitro, yielding proteins with an electrophoretic pattern resembling t ...	1982	6292446
effect of tunicamycin on rotavirus assembly and infectivity.	bovine rotavirus grown in the presence or absence of tunicamycin was analyzed with respect to yield of infectious virus, the ratio of complete to incomplete particles, and polypeptide composition. tunicamycin at a concentration of 1 microgram/ml reduced virus yields by 4 logs and completely prevented the incorporation of [3h]uridine into complete rotavirus particles, as determined by cesium chloride gradient analysis. concomitant with a reduction in complete particles, three rotavirus polypeptid ...	1982	6292451
[acute gastroenteritis epidemic in adults caused by rotaviruses].		1982	6287728
human rotavirus rna prepared from stool samples by a simple procedure suitable for the determination of electropherotypes.		1982	6281287
the differentiation of calf rotaviruses by plaque morphology and serum neutralization.		1982	6281378
isolation and genetic characterization of temperature-sensitive mutants of simian rotavirus sa11.		1982	6285605
demonstration of rotavirus antigen in trypsin-digested paraffin tissue sections by immunofluorescence.	trypsin-digested paraffin tissue sections were used to demonstrate rotavirus particles in small intestinal epithelial cells of dirrheic neonatal calves, using direct fluorescent-antibody assay. the results were compared with other techniques to demonstrate rotavirus particles in tissue sections by immunofluorescence. enzyme treatment of deparaffinized tissue sections gave excellent results with distinct specific fluorescence and minimal background. prior staining of the tissue section with mayer ...	1982	6285772
[acute gastroenteritis caused by rotavirus (author's transl)].	a prospective study of 87 children hospital admitted for acute gastroenteritis [age] with ages between 24 days and three years and a control group of 32 children with ages between six months and three years, is presented. in all cases a virological study of stools is made by electron microscopy [e/m]. in 63 percent of the 87 patients with age, viruses were isolated, being rotavirus the most common, 45.9 percent, a similar incidence as that found in developed countries. the clinical course and an ...	1982	6285787
development of a method for detection of human rotavirus in water and sewage.	the simian rotavirus sa11 was used to develop a simple, reliable, and efficient method to concentrate rotavirus from tap water, treated sewage, and raw sewage by absorption to and elution from filterite fiberglass-epoxy filters. sa11 adsorbed optimally to filterite filters from water containing 0.5 mm alcl3 at ph 3.5. filter-bound virus was eluted with 0.05 m glycine-naoh supplemented with 10% tryptose phosphate broth at ph 10. sa11 was quantitated by plaque assay, whereas human rotavirus was de ...	1982	6285825
detection of rotavirus antibody by inhibition of reverse passive hemagglutination.	a reverse passive hemagglutination inhibition (rphi) test was developed for detecting rotavirus antibody in patients' sera. sheep erythrocytes coated with guinea pig antibody against nebraska calf diarrhea virus (ncdv) were readily hemagglutinated by ncdv in a reaction called reverse passive hemagglutination (rpha). inhibition of this rpha reaction was used to detect the presence of rotavirus antibody in patients' sera which cross-reacted with ncdv. the sensitivity of rphi was consistently at le ...	1982	6313739
assay of human rotavirus antibody by immune adherence hemagglutination with a cultivable human rotavirus as antigen.	a cultivable human rotavirus, wa, was propagated in ma-104 cells and used as an antigen in immune adherence hemagglutination (iaha) and complement fixation (cf) tests. iaha antibody titers of sera from normal humans were found to be eightfold higher than cf antibody titers. the iaha antibody determination with the wa antigen is considered to be especially useful for the seroepidemiological studies of human rotavirus infections.	1982	6313741
[infectious gastroenteritis in childhood (author's transl)].	stool specimens from 126 children with acute gastroenteritis and from 42 controls were screened bacteriologically and virologically including electron-microscopy. rota viruses were found in 36.5% of the children with diarrhoea, enteropathogenic e. coli in 13.5% and candida in 7.1%. these pathogens were not found in the controls. enteroviruses were found in almost the same frequency in both groups (13.5% in children with gastroenteritis, 16.6% in the controls). the etiologic role of adenoviruses, ...	1982	6287245
the effects of relative humidity and temperature on the survival of human rotavirus in faeces.	the effects of relative humidity and temperature on the survival of human rotavirus in a thin layer of faeces on an impervious surface and on absorbent material was investigated using the indirect immunofluorescence technique in llc-mk2 cells to titrate infectivity. rotavirus was found to be very stable at low and high relative humidities but not in the medium range of relative humidity. rotavirus infectivity was lost more rapidly under all humidities at 37 degrees c than at 4 degrees or 20 degr ...	1982	6287970
a general strategy for cloning double-stranded rna: nucleotide sequence of the simian-11 rotavirus gene 8.	using simian-11 rotavirus rna, a strategy has been developed for the production of full length cloned copies of the genes of double-stranded (dsrna) viruses. genomic rna segments were polyadenylated and reverse transcribed to yield a mixture of full length cdna copies of both possible polarities. the cdnas were annealed, filled in to complete any partial copies, tailed and inserted into the psti site of pbr322 using dg/dc tailing. cloned rotavirus cdna gene copies were assigned to genomic rna se ...	1982	6296764
propagation of bovine rotavirus by cats and dogs.	spf cats were experimentally infected orally with bovine rotavirus. all of them excreted virus over a period of at least two weeks after inoculation. seroconversion was observed after one week for all the animals, but it did not stop viral excretion or prevent further excretion of the same or another rotavirus strain given later. cats or dogs kept in the same cage as inoculated animals became infected and excreted virus, but seroconversion was not observed in these contact animals. none of the a ...	1982	6309066
[epidemic of acute gastroenteritis due to rotavirus in lianyungang city].		1982	6309401
[studies on the etiology of automnal acute gastroenteritis in kunming. i. a preliminary report on the detection of rotavirus].		1982	6303613
identification of the rna segments encoding some structural polypeptides of nebraska calf diarrhea virus.		1982	6312525
[detection of rotavirus in healthy malagasian children].		1982	6312908
[epidemiological study of rotavirus infections in premature infants].		1982	6320707
intestinal changes in gnotobiotic piglets experimentally inoculated with porcine rotavirus.	intestinal lesions were studied in 8 gnotobiotic piglets orally given porcine rotavirus by light microscopy, scanning electron microscopy and transmission electron microscopy. five of these piglets were killed 6, 12, 18, 24 and 48 hours after inoculation, respectively. of the other 3 piglets, one died 60 hours and two became moribund 96 and 114 hours after inoculation, respectively. all the infected piglets showed diarrhea between 18 and 24 hours after inoculation. examination of the small intes ...	1982	7133149
[the aerobic bacterial intestinal flora of various wintering geese species].	the aerobic fecal flora of wintering brent goos (branta bernicla), barnacle goose (branta leucopsis), greylag goose (anser anser), white-fronted goose (anser albifrons), pink-footed goose (anser brachyrhynchus), and bean goose (anser fabalis) was studied. there were no specific differences between the various geese. bacterial counts were in the range of 10(5)-10(7) cpu per gram of feces. neither pathogenic bacteria nor rotavirus could be detected in the fecal samples of the wintering geese, so t ...	1982	7136353
diarrhea in breast-fed and non-breast-fed infants.	during the first year of life a group of babies was prospectively observed for diarrhea and for fecal carriage of heat-labile toxigenic bacteria, with or without colonization factor, and rotavirus. approximately half of the babies were breast-fed for the first six months of life. there was no difference between groups (breast-fed vs non-breast-fed) in number of babies who had diarrhea during any two-month period. nor was there any difference between groups in the number of babies who had diarrhe ...	1982	7145548
diarrhoea in foals associated with rotavirus.		1982	7147670
epidemiology of rotavirus diarrhea in yogyakarta, indonesia, as revealed by electrophoresis of genome rna.	nine different electropherotypes of rotaviruses occurred among 85 children with rotavirus diarrhea in yogyakarta, indonesia. eighty percent of the electropherotypes had a "long" rna pattern characteristic of human subgroup 2.	1982	7153319
clinical features of rotavirus gastroenteritis.		1982	7185988
[sero-epidemiologic study of the occurrence of rotavirus infection in the roman population].		1982	7187035
[rotavirus seroepidemiology: predominance of precipitating antibodies in serum of children between the ages 0 to 12].		1982	7187042
serological survey on rotaviral antibody in the hungarian swine population by optimized counter-current immuno-electrophoresis (cciep).		1982	7187805
[serological signs of nosocomial infections by rotavirus in a pediatric ward for infectious diseases].		1982	7188245
diarrhoeal problems in southeast asia.	diarrhoea up till now is still a major problem in southeast asia with high morbidity and mortality, particularly among children under 5 years of age, with the peak in children between 6 - 24 months. in indonesia, in 1981, it was estimated that there are 60 million episodes with 300,000 - 500,000 deaths. in the philippines, diarrhoea ranks as a second cause of morbidity (600 per 100,000 in 1974) and second cause of infant mortality (5 per 1,000 in 1974). in thailand, in 1980, the morbidity rate w ...	1982	7163834
a long term study of rotavirus infection in thai infants and children with diarrhoea.	during 1977-1980, human rotavirus was detected by electron microscopic technique in the stool of 55 (30.7%) of 179 patients, hospitalized in bangkok metropolis. characteristic particles 65-70 nm in diameter, round shape, resembling a little wheel with radius capsomeres from the dense core. other some adenovirus and small virus particles were seen, 2.8% and 2.2% respectively. seasonal distribution among thai patients with rotavirus infection mostly occurred in january (58.8%). the youngest patien ...	1982	7163842
morbidity and mortality study on diarrhoeal diseases in north jakarta - an urban area.	diarrhoea including its interaction with undernutrition is one of the most important health problems and a major cause of death in young children in developing countries including indonesia. to quantify the problems and to have comparison with results of other diarrhoeal surveys in indonesia as well as in other developing countries, a survey was done in urban community comprising a population of 5,115 living in a relatively good area which was not a slum, nor wealthy area. the incidence of diarr ...	1982	7163847
[role of viruses in acute gastroenteritis].	in a two years screening carried out on florentine children we confirmed that rotaviruses are the most important ethiological agents of acute gastroenteritis in infants less than two years old, irrespective of sex. we had evidence that rotavirus diarrhea does not occur with the same incidence every year. rotavirus gastroenteritis is characterized by profuse diarrhea, vomiting, fever and often by respiratory symptoms. severe complications have not been observed.	1982	7170197
diarrhea in day care centers.		1982	7177896
[useful and superfluous measures in the treatment of infant diarrhea].	in acute diarrhea of infancy we distinguish between infectious and noninfectious causes. in the latter we know some autosomal recessive disorders, e.g. the glucose-galactose-malabsorption, the lactase deficiency as well as the sucrase-isomaltase deficiency. in addition the most frequent acquired disorders like the cow's milk protein intolerance and celiac disease contribute also to the group of noninfectious causes of diarrhea. here the most effective therapy consists of the elimination of the t ...	1982	7178037
etiology of hospital-acquired infectious diarrhea.		1982	7181595
enterotoxigenic escherichia coli and other enteropathogens in paediatric diarrhoea in addis ababa.	this study was performed during two weeks among 86 paediatric outpatients of poor socio-economic background. a control group comprised 60 healthy children. enterotoxigenic escherichia coli (etec) was the most common diarrhoeal agent isolated (26%). strains of etec producing heat-labile (lt) only or lt and heat-stable (st) enterotoxin were isolated from 11% each and etec producing st only from 4% of the patients. etec was also found not infrequently among controls (10%). etec with o-antigens 78, ...	1982	6753473
colonization of the upper jejunum by enteropathogenic and enterotoxigenic escherichia coli in paediatric diarrhoea.	aspirated fluid from the upper jejunum was obtained by intubation of 27 children with diarrhoea and 7 control children without diarrhoea. the aspirated jejunal fluid was analysed for total counts of viable aerobic and anaerobic microorganisms. gram-negative aerobic bacteria were typed biochemically and analysed for the production of heat-labile and heat-stable enterotoxin. enteropathogenic escherichia coli (epec) strains of serogroups 0111, 055 and 0127 were detected in the stools of nine patien ...	1982	6753475
infectious gastroenteritis in bone-marrow-transplant recipients.	we prospectively evaluated infections with several gastrointestinal pathogens in patients undergoing bone-marrow transplantation, in an attempt to correlate infection with morbidity and mortality. thirty-one of 78 patients (40 per cent) were infected with one or more of the following enteric pathogens during the study: adenovirus (12 infections), rotavirus (nine), coxsackievirus (four), or clostridium difficile (12). several patients were infected with more than one pathogen. infection correlate ...	1982	7038501
studies with an unclassified virus isolated from diarrheic calves.	a transmissible agent (breda agent) was isolated from a calf with diarrhea and shown to be infectious by inoculation orally into gnotobiotic and conventionally reared calves. the "breda" agent had the morphology of a virus and possessed a hemagglutinin. antigenic studies showed the virus to be antigenically different from bovine coronavirus, parainfluenza 3 virus, bovine rotavirus, bovine parvovirus and bovine pestivirus (bvd). attempts to culture the virus in cell or organ cultures or in embryo ...	1982	7051518
theory and practice of immunoprophylaxis in swine.		1982	6757217
diarrhoea in swedish infants. aetiology and clinical appearance.	ninety-five children with gastroenteritis admitted at the department of paediatrics, malmö general hospital, malmö, sweden, starting november 1978 through october 1979 were studied. patients were examined clinically and the course of their disease was followed. stool specimens from all patients were analysed by routine bacteriology and rotavirus identification was done by immune-electroos-mophoresis. fifty-eight per cent of patients had rotavirus. campylobacter was found in 6%, enteropathogenic ...	1982	6760665
rotavirus infections in a special-care baby unit.		1982	6764499
mechanisms and mediators of intestinal secretion in the small intestine.		1982	6764511
enzyme immunoassays for the detection of infectious antigens in body fluids: current limitations and future prospects.	enzyme immunoassays are attaining increased usage for the direct detection of microbial antigens in body fluids. advantages of enzyme immunoassays include a high degree of sensitivity resulting from the inherent magnification of the enzyme-substrate reaction and the use of objective end points without the need for radioactivity. enzyme immunoassays have been developed for the reliable detection of several important microbial antigens in body fluids, including antigens of rotavirus, hepatitis b v ...	1982	6803327
surveillance of patients attending a diarrhoeal disease hospital in bangladesh.	in october 1979 a surveillance system was set up at the international centre for diarrhoeal disease research, bangladesh, hospital at dacca to study a 4% systematic sample of the 100 000 patients with diarrhoea who come to the hospital for care each year. from december 1979 to november 1980 inclusive, 3550 patients were studied. a recognised pathogenic organism was identified for 66% of patients screened for all pathogens, one-third of whom had a mixed infection with two or more agents. enteroto ...	1982	6812801
[fecal detection of rotavirus and other enteropathogens in infants less than a year old, with acute diarrhea].		1982	6817391
[cryptosporidium: a "new" factor in the aetiology of neonatal diarrhoea in calves].	in recent years, cryptosporidium, a coccidian parasite of the intestine, has been associated with neonatal diarrhoea in calves. current knowledge of the organism with regard to classification, host-specificity, life cycle, pathology, incidence, clinical treatment, prevention and diagnosis is reviewed in the present paper. in addition, cryptosporidium infection in calves in the netherlands is reported for the first time. the typical 4 micrometer oocysts were observed on microscopic examination of ...	1982	7123530
rotavirus gastroenteritis and weather.	during 5.5 years of a study in washington, d.c., hospitalizations of children for rotavirus gastroenteritis tended to be more common after a month of cold or dry weather than after a corresponding calendar month of warm or wet weather. overall, there were 84% more (178 versus 97) inpatients with rotavirus gastroenteritis after a set of relatively colder individual months taken as a group than after an equal number of warmer corresponding calendar months taken as a group. comparable differences w ...	1982	7130360
study of rotavirus diarrhea in a north indian community.		1982	7160902
morphology of intestinal changes in pigs experimentally infected with porcine rota-virus and two porcine corona viruses.		1982	6959242
stool electrolyte composition in relation to etiology in acute gastroenteritis.		1982	6754604
[rotavirus infections in a pediatric section of an infectious disease hospital department supervised in march-may 1981].		1982	7144711
carbohydrate malabsorption in infants with rotavirus diarrhea.	we studied a group of patients with rotavirus diarrhea to determine the association of carbohydrate malabsorption during diarrhea with the degree of acidosis and severity of purging. unlike enterotoxigenic diarrhea in which the metabolic acidosis is due to loss of bicarbonate in an alkaline stool, patients with rotavirus develop a metabolic acidosis while passing an acid stool with little detectable bicarbonate. also unlike enterotoxigenic diarrhea, rotavirus stool contains large quantities of r ...	1982	7148733
human rotavirus infection in malaysia. iii. a one year survey on the prevalence of rotavirus enteritis in children.	the prevalence of human rotavirus enteritis in children admitted to the gastroenteritis ward of the kuala lumpur general hospital was studied in 1982. human rotavirus in the stool of the patients was detected by enzyme-linked immunosorbent assay. the survey showed that rotavirus enteritis in children were observed throughout the year, with two broad peaks of rotavirus infection occurring around march and september. the lowest incidence was recorded in july, however, no prolonged period of low pr ...	1983	6673122
[rotavirus infection in children--clinical aspects].		1983	6847327
influence of malnutrition and alterations in dietary protein on murine rotaviral disease.	the possible correlation between malnutrition and degree of severity of rotavirus-associated infantile diarrhea which appears to occur in human populations was studied using a mouse model. to determine the effects of general malnutrition or altered levels of dietary protein, female mice were fed throughout pregnancy and infection periods with diets diluted with 0, 300, or 600 g glucose/kg, designated as normal nutrient to calorie ratio (n/c) diet, 70% n/c diet, or 40% n/c diet or with diets cont ...	1983	6867015
[susceptibility of the dromedary (camelus dromedarius) to rotavirus infection].		1983	6672882
necrotizing enterocolitis--a 5-year experience.	with the increased survival of very-low-birth-weight neonates, necrotizing enterocolitis (nec) represents one of the most frequent life-threatening conditions for this population. nec was diagnosed on 24 occasions during a 5-year period (1977-81) at the neonatal intensive care unit, kaplan hospital, rehovot. these 24 nec cases, confirmed by radiological, surgical or pathological findings, were analyzed in a retrospective study. the mean weight was 1,450 g and the mean gestational age 32 weeks. o ...	1983	6662689
[evidence of the viral (rotavirus) etiology of acute gastroenteritis in children].		1983	6664730
microtiter solid-phase radioimmunoassay for detection of human calicivirus in stools.	a microtiter solid-phase radioimmunoassay (ria) was developed for detection of human calicivirus in stool specimens. seventy-eight stool specimens were tested by ria. all 17 specimens positive for human calicivirus by electron microscopy (em) were also positive by ria. in addition, of 21 specimens obtained from an outbreak of caliciviral gastroenteritis, 11 were positive by ria but negative by em. of 20 specimens positive for rotavirus by em and 20 nondiarrheic specimens with no virus, 2 and 1, ...	1983	6833476
rotavirus infections in a pediatric clinic.		1983	6840056
[rotavirus infections--epidemiological aspects].		1983	6847329
[value of the detection of rotaviruses by an immunoenzymatic technic in the etiologic diagnosis of pediatric gastroenteritis].		1983	6670819
influence of temperature and relative humidity on human rotavirus infection in japan.	a climatologic analysis of human rotavirus infection in inpatients with acute diarrhea was conducted over a seven-year period. the infection frequency appeared to be related to temperature, but not to relative humidity. human rotavirus infection was found to appear abruptly when the mean temperature of any 10-day period became less than 5 c (november or december), reached a peak when it was less than 0 c (january and february), and waned when it became greater than 20 c (june and july) in the ci ...	1983	6822748
differential diagnosis of scouring in lambs.		1983	6832843
rotavirus outbreak in a newborn nursery: report of cases.		1983	6618920
rotavirus infection in brazilian children with acute enteritis: a seasonal variation study.	enzyme immunoassay (eia) and electron microscopy (em) were utilized to investigate the presence of rotavirus in feces of 388 children with acute enteritis hospitalized at the hospital santa casa de misericórdia in porto alegre, brazil. the survey covered 12 months, beginning in july 1981. there were 162 rotavirus-positive cases (41.8%). during the period of the study rotavirus was detected throughout the year, but there was a striking seasonal variation (78.1% of cases) during january 1982.	1983	6625073
total serum ige levels in venezuelan schoolchildren.	seventy-eight disease-free children were evaluated by prist for total serum ige in order to establish the normal range for this immunoglobulin and assess its utility, in tropical climates, such as venezuela, as a diagnostic tool for clinical allergy. seventy-eight normals were selected from a group of 1053 children, aged 7-12 years from fourteen schools in caracas. exclusion from the normal pool was based on nationality, and on historical, clinical, and/or laboratory evidence of atopic and/or in ...	1983	6640887
rotavirus-induced electrolyte losses in a patient with ileostomy.		1983	6637923
rotavirus infection in black and white newborns.	rotavirus infection in asymptomatic black and white newborns in maternity hospitals was studied by electron microscopy of stools. none of the 37 white and 30 (49%) of the 61 black babies excreted rotavirus (p less than 0.0005). the high incidence of neonatal infection in blacks, who have a low incidence of rotavirus diarrhea in infancy, suggests that neonatal infection might result in immunity.	1983	6662672
[frequency of acute infectious gastroenteritis caused by rotavirus in children of various populations of the mexican republic].		1983	6871004