PMID(sorted ascending)
molecular and antigenic characterization of porcine rotavirus ym, a possible new rotavirus 1983, we isolated a porcine rotavirus (strain ym) that was prevalent in several regions of mexico, as judged by the frequency of its characteristic electropherotype. by a focus reduction neutralization test, rotavirus ym was clearly distinguished from prototype rotavirus strains belonging to serotypes 1 (wa), 2 (s2), 3 (sa11), 4 (st3), 5 (osu), and 6 (ncdv). minor, one-way cross-neutralization (1 to 5%) was observed when antisera to the various rotavirus strains were incubated with rotavirus ...19882845146
phenotypic mixing during coinfection of cells with two strains of human rotavirus.coinfection of ma-104 cells with serotype 1 (wa strain) and serotype 3 (p strain) human rotaviruses resulted in progeny viruses that were phenotypically mixed in their major outer-shell structural-protein vp7 and in the genome segment that encodes this protein (segment 9). segments from the wa virus predominated in these progeny whether they were of parental or reassortant genotype. neutralization with monoclonal antibodies specific for vp7 proteins of the coinfecting viruses caused an alteratio ...19882845148
duration and pattern of asymptomatic rotavirus shedding by hospitalized children.fecal shedding of rotaviruses by infants and children without gastrointestinal symptoms has recently been recognized, but little is known about the duration or frequency of virus shedding in such patients. we therefore evaluated rotavirus shedding by 16 infants and children during the course of their prolonged hospitalizations. repeated fecal specimens were obtained from these patients and examined for the presence of rotavirus by means of immunoassay, electron microscopy and polyacrylamide gel ...19882845347
field trial of rhesus rotavirus vaccine in infants.orally administered rhesus rotavirus vaccine (rrv) was evaluated in a placebo-controlled study in 176 infants (ages 2 to 4 months). eighty-eight infants received a dose of 10(4) plaque-forming units of the vaccine, and 88 received the placebo. rrv was well-tolerated but mildly reactogenic in the 10 days after vaccination. there were mild febrile reactions (greater than or equal to 38 degrees c rectally) in 40% of the vaccinees and in 16% of the placebo recipients (p = 0.001). more of the vaccine ...19882845349
[enzyme immunoassay for rotavirus using nylon as the solid phase].an enzyme-linked immunoassay (eia) to detect rotavirus in stools is described. antibodies prepared in rabbits were immobilized on small nylon cubes as capture phase and enzyme conjugated antibodies were used to reveal the reaction. the conjugate was prepared with horseradish peroxidase by the nakane periodate oxidation method. the solid phase consisted of 3 mm nylon cubes (66 cnl du-cilo) previously submitted to partial acid hydrolysis to liberate amino-reactive groups. glutaraldehyde was employ ...19872845474
laboratory investigations on rotavirus in infantile gastroenteritis in jamaica.between march 1982 and march 1983 rotaviruses were detected in faecal specimens from 193 (19%) of 1020 jamaican children under 5 years old with acute gastroenteritis. the excretion of rotaviruses in a control group of non-diarrhoeal children was only 1.4%. rotaviruses were detected more frequently in the cooler months of the year; the prevalence ranged from 1.7% in august to 46% in january. in a comparison of the elisa technique and electron microscopy for investigating rotavirus infections, the ...19882845610
rotavirus diagnostic kits. 19882845628
nucleotide sequence of uk bovine rotavirus segment 4: possible host restriction of vp3 genes.the bovine uk and simian sa11 rotaviruses are commonly used vp7-type reference strains. since the surface protein vp3 is a significant neutralization antigen, it is important to fully characterize the vp3 types associated with current reference strains. here we present the complete nucleotide and predicted amino acid sequence of vp3 from uk rotavirus (vp7 type 6) and compare it to the published sequences of sa114fm and rv-5. we also compare the deduced amino acid sequence covering the trypsin cl ...19882845645
structure and protein composition of the rotavirus replicase understand the role of viral proteins in the replication of rotavirus rna, we have characterized the structure of subviral particles (svps) that synthesize double-stranded rna (ds rna). pulse-labeling of newly made rna in infected cells showed that rotavirus ds rna was synthesized either in single-shell (ss)-like particles or in precursor particles that rapidly mature into ss particles. experiments using a cell-free system demonstrated that most replicase particles containing newly made ds rn ...19882845649
[discovery and quantitative determination of intestinal viruses by the detection of viral antigens in infected cells using an immunoperoxidase method].the use of immunoperoxidase method for detection of virus antigens in a fixed infected cell monolayer on microplates (cellular immunoperoxidase method, cipm) for poliomyelitis and hepatitis a virus as well as for human and simian sa ii rotaviruses is described, as well as the method of quantitation of rotaviruses by counting of virus multiplication foci. the cipm combines the advantages of the methods based on the infectivity determinations and of immunochemical methods, and may be used both for ...19882845670
polyethylene glycol precipitation for recovery of pathogenic viruses, including hepatitis a virus and human rotavirus, from oyster, water, and sediment samples.polyethylene glycol 6000 precipitation was found to be an effective concentration method that enhanced the chances for detecting human virus pathogens in environmental samples. percent recoveries from eluates of fresh and estuarine waters with 8% polyethylene glycol 6000 averaged 86 for hepatitis a virus, 77 for human rotavirus wa, 87 for simian rotavirus sa11, and 68 for poliovirus. percent recoveries of 97, 40, 97 and 105, respectively, for the same viruses were obtained from oyster eluates by ...19882845860
neonatal calf diarrhea induced by rotavirus.this presentation summarizes the results of a comprehensive study on rotaviruses isolated in italy from calves and rabbits affected by neonatal diarrhea. the results clearly indicated that rotavirus infection is widespread and supported the evidence for an etiologic role of these viruses in neonatal diarrhea. the evidence of differences in virulence among bovine rotaviruses appeared also to be confirmed. conventionally reared calves were fully susceptible to the experimental infection induced by ...19882846230
faecal excretion of rotavirus and other enteropathogens in newborns of the high and low socio-economic stratum in santiago, chile.faecal excretion of enteropathogens was studied in newborns in their first week of life. rotavirus was investigated in 225 neonates, of whom 107 belonged to the low socio-economic stratum (ses) and 118 to the high ses. half of each group were delivered by caesarean section. rotavirus was detected in 10 infants (4.4%). eight of them had been in the same ward and excreted the same viral electrophoretype. enteropathogenic bacteria were isolated from 8 out of 57 (14.0%) newborns. positive cultures w ...19882846331
immunological aspects of interaction between rotavirus and the intestine in infancy.rotaviruses are important pathogens causing severe diarrhoea in human infants and young animals. available information on the pathogenic mechanisms of and the immune response to rotaviruses is reviewed here. studies in our laboratory using the suckling mouse model have focused on elucidating the nature of interaction between the virus and the gut, and on the importance of t and b cell mediated immunity in protection and recovery from the disease. our data suggest that the age dependence of mouse ...19882846434
primary sequence domains required for the retention of rotavirus vp7 in the endoplasmic reticulum.rotavirus vp7 is a membrane-associated protein of the endoplasmic reticulum (er). it is the product of rotavirus gene 9 which potentially encodes a protein of 326 amino acids that contains two amino terminal hydrophobic domains, h1 and h2, each preceded by an initiation codon. comparison of the size of products derived from altered genes containing coding sequences for both h1 and h2 with those lacking the h1 sequence ('dhl' mutants), indicates that initiation takes place at m30 immediately prec ...19882846584
relative frequencies of rotavirus serotypes 1, 2, 3, and 4 in venezuelan infants with gastroenteritis.we have used a recently developed monoclonal antibody enzyme-linked immunosorbent assay (k. taniguchi, t. urasawa, y. morita, h. b. greenberg, and s. urasawa, j. infect. dis. 155:1159-1166, 1987) for serotyping rotaviruses recovered from 134 venezuelan infants over a period of 15 months. one hundred and nine of the specimens were typed with the following distribution: serotype 1, 48%; serotype 2, 16%; serotype 3, 22%; and serotype 4, 14%. three specimens reacted with two different monoclonal ant ...19882846637
isolation of a bovine rotavirus with a "super-short" rna electrophoretic pattern from a calf with diarrhea.a rotavirus with a "super-short" rna electropherotype was isolated from a calf with diarrhea and was designated vmri strain. segments 10 and 11 of this rotavirus migrated more slowly than did those of bovine rotavirus strains ncdv, b641, and b223. the electrophoretic pattern of the vmri strain was similar to that reported for rotaviruses with super-short rna electropherotypes from humans and rabbits. northern (rna) blot hybridization indicated that gene 11 of the vmri strain was altered and migr ...19882846639
comparison of nine commercial immunoassays for the detection of rotavirus in fecal hundred fecal specimens obtained from patients with acute gastroenteritis were tested for rotavirus with nine commercial immunoassays to evaluate the sensitivity, specificity, predictive value, and diagnostic accuracy of these assays. kits evaluated included two monoclonal antibody-based enzyme immunoassays (eias) (rotaclone and pathfinder rotavirus), three polyclonal antibody-based eias (rotavirus immunoassay, rotazyme ii, and wellcozyme rotavirus), and four latex agglutination assays (rota ...19882846645
incidence of enteric adenoviruses among children in thailand and the significance of these viruses in countries with temperate climates, enteric adenoviruses have been shown to be a substantial cause of pediatric gastroenteritis. to determine the incidence of adenovirus infection in a tropical climate, stools were collected from children under age 7 during a 1-year period at an outpatient clinic in bangkok, thailand. stools from 1,114 children with gastroenteritis and from 947 children without gastroenteritis were tested. each stool was tested for adenovirus group antigen and for specific ent ...19882846648
serum and salivary antibodies as indicators of rotavirus infection in neonates. 19882846706
lack of genomic reassortment following infection of infant rats with group a and group b rotaviruses. 19882846707
factors affecting antibody response of newborns to repeated administration of rotavirus rit 4237. 19882846813
genetic stability of rotaviruses recovered from asymptomatic neonatal infections.the sequence of the vp7 gene from 19 rotavirus strains recovered from asymptomatically infected newborn infants was determined by direct analysis of transcript rnas synthesized from virus present in the stool. for five viruses the entire vp7 gene was sequenced, whereas in the remaining instances only a portion of the gene could be sequenced. in 19 specimens collected over a 4-year period, only five nucleotide substitutions were detected. none of them resulted in an amino acid substitution. exami ...19882846889
routine testing for rotavirus and cryptosporidium in children with gastro-enteritis. 19882847337
[resistance and the local immune response to rotavirus infection after intrauterine immunization of calves and in non-immunized calves]. 19882847390
influence of immunomodulatory agents on bovine humoral and cellular immune responses to parenteral inoculation with bovine rotavirus vaccines.sodium diethyldithiocarbamate (dtc), mycobacterium cell wall extract (mcwe, regressin), killed corynebacterium parvum (c. parvum, immunoregulin) and muramyldipeptide (mdp) were each combined with purified, live bovine rotavirus and inoculated into 3 month-old holstein-friesian calves in order to examine their ability to potentiate specific humoral and cellular immune responses. the vaccinated calves were boosted twice at 3 and 6 weeks after initial vaccine inoculation. the rotavirus was administ ...19882847397
in vitro assembly of the outer capsid of bovine rotavirus is calcium-dependent.the nucleocapsid protein (vp6) and outer capsid glycoprotein (vp7) of bovine rotavirus (brv) assemble in vitro, in 0.01 m tris-hcl, ph 8.0, 50 mm cacl2, into smooth particles resembling double-shelled brv. that the two proteins interact is demonstrated by the immunoprecipitation of both by antibody directed against either vp6 or vp7. the calcium-dependence, particle morphology, and immunoreactivity in elisa suggest that vp7 is presented authentically on the outer capsid. the implications for rot ...19882847415
the structure of tubes of bovine rotavirus nucleocapsid protein (vp6) assembled in vitro.the structure of tubes of reassembled nucleocapsid protein (vp6) from bovine rotavirus (brv) was determined using optical diffraction of electron micrographs. the tubes consist of a five-start helix of hexagons, with 38 hexagons per helix in a true repeat of three turns. the morphological subunits comprising the hexagons are probably elongated trimers. the structure of naturally occurring tubes (d. chasey and j. labram, 1983, j. gen. virol. 64, 863-872) was also examined and shown to be similar ...19882847425
isolation of bovine colostral lymphocytes: in vitro blastogenic responsiveness to concanavalin a and bovine order to study the in vitro proliferative activity of bovine colostral lymphocytes, the lacteal secretions from four holstein heifer cows were collected at days 1 and 2 after calving for lymphocyte isolation. a density gradient centrifugation using a ficoll-diatrizoate solution (sp gr 1.084 g/ml) allowed to isolate a cell population which consisted mainly of lymphocytes. the cell cultures incubated with con a showed a significant in vitro lymphoblastic transformation, although the mitogenic r ...19882847626
[morphologic studies of virus infection of the intestinal tract--virus replication and cytopathology in cell cultures and enterocytes in piglets. 1. ultrastructure of intestinal epithelium without viral infiltration and in rotavirus infection]. 19882847679
[morphologic studies of virus infection of the intestinal tract--virus replication and cytopathology in cell cultures and enterocytes. 3. replication of porcine adenoviruses in cell culture compared to that of rota- and coronaviruses]. 19882847681
follow-up on thai infants for rotavirus antigen and antibody in feces. 19882848057
the effects of host age, virus dose, and virus strain on heterologous rotavirus infection of suckling suckling cd-1 mice, born to seronegative dams, were orally inoculated with a number of animal and human rotaviruses. simian (sa11), rhesus (rrv), and bovine (b223) rotaviruses were found to replicate and cause severe disease. canine (k9), bovine (b641), and human (wa) rotaviruses either replicated minimally and caused minimal disease (k9, b641) or failed to replicate or cause disease (wa). the features of sa11 infection of mice were examined in greater detail. suckling mice were su ...19882848173
validity of an enzyme-linked immunosorbent assay with serotype-specific monoclonal antibodies for serotyping human rotavirus in stool specimens.we recently developed a method for serotyping human rotavirus (hrv) by an enzyme-linked immunosorbent assay with hrv serotype-specific neutralizing monoclonal antibodies (elisa serotyping). in the present study this method was compared with the fluorescent focus neutralization test with serotype-specific rabbit antisera (nt serotyping) in the sensitivity and specificity of the test. direct serotyping of hrvs which were contained in stool specimens indicated that while only 37% of the samples wer ...19882848180
[presence of the rotavirus antigen in newborn infants at maternity hospitals in moldavia].studies conducted on 417 feces samples collected from newborn infants from seven maternity homes revealed the presence of rotavirus in 1.2 to 9.5% of the subjects. the infants get infection during the first 24 to 48 hours of life (1.8%) and the positivity rate reaches a peak the 7th day (9.7%). enteroviruses were found in 3.4% and enterobacteria in 11.8% of the samples.19882848357
[detection of the rotavirus antigen in clinical material and environmental objects using variants of immunoenzyme analysis]. 19882848372
[oral vaccination with live attenuated rotavirus].a randomized, double-blind trial was performed to assess serological response and clinical protection for acute gastroenteritis due to rotavirus in 103 children aged 6 to 18 months, after a single dose of rit 4237 live attenuated bovine rotavirus vaccine or placebo. seroconversion, determined by enzyme-linked immunoabsorbent assay (elisa), was significantly greater in initially seronegative vaccines compared with control group (p less than 0.0001); clinical protection rate was low in this study ...19882848432
atypical rotaviruses in australian pigs.atypical rotaviruses of two different electropherotypes were identified by page in 16 out of 237 (5.3%) diarrhoeic faecal samples from piglets. a cdna probe derived from a group b rotavirus hybridized strongly to two samples (of 3 tested) with electropherotypes suggestive of groups b or e, identifying them as group b. the electropherotype(s) of seven samples were typical of group c. by immunofluorescence, antibodies to group b, c, and e rotaviruses have been detected in sera from australian sows ...19882848477
protease inhibitors suppress the in vitro and in vivo replication of rotavirus.rotaviruses are major causes of infectious gastroenteritis in humans and other animals. we found that a variety of protease inhibitors suppressed the replication of the sa-11 strain of rotavirus in ma-104 cell cultures. three of these compounds, leupeptin, pentamidine, and bis (5-amidino-2-benzimidazolyl) methane (babim) also restricted the intestinal replication of the murine strain of rotavirus when protease inhibitor and virus were administered simultaneously to suckling mice. repeated admini ...19882848866
viral hepatitis and gastroenteritis transmitted by shellfish and water.the epidemiology and clinical presentation of water-borne viral diseases, including shellfish-associated viral illnesses, are discussed in this article. hepatitis a virus, non a-non b hepatitis and the agents of viral gastroenteritis, norwalk agent, snow mountain agent, rotavirus, the small round viruses, caliciviruses, and astroviruses are included. the technical problems associated with evaluating the viral contamination of water or shellfish are noted.19872848882
human-rhesus reassortant rotavirus vaccines: safety and immunogenicity in adults, infants, and children.human-rhesus reassortant rotavirus vaccines derived from rhesus rotavirus and human rotavirus serotypes 1, 2, or 4 were administered to adults, children, and infants after they had been given sodium bicarbonate buffer. over 70% of infants and children developed antibody responses to or shed the candidate vaccine viruses. individuals with prevaccination serum neutralization antibody titers less than or equal to 1:160 had significantly (p less than .0001) higher response rates than did individuals ...19882848902
reinfections and rotavirus serotypes in belém, brazil (preliminary report). 19882849197
molecular epidemiology of human rotavirus infections based on genome segment variations in viral strains.molecular epidemiology of rotavirus infections in 621 hospitalized children was investigated by analysis of migration patterns of viral genomic ribonucleic acid (rna) segments by electrophoresis in polyacrylamide gels. based on migration patterns of rna segments of 184 rotavirus strains, seven different electropherotypes were identified: 146 (79.3%) strains were "long," and 38 (20.7%) were "short" electropherotypes; 61% belonged to a single dominant "long" electropherotype, which persisted throu ...19882849633
investigation of hospital-acquired rotavirus gastroenteritis using rna electrophoresis.rotavirus is a common cause of diarrhoea both in the community and in the hospital. because of this, it may be difficult to determine whether crossinfection has occurred in the hospital, an important finding as review of hygienic techniques and ward closure may be indicated. we therefore investigated the use of polyacrylamide gel electrophoresis (page) of the rotavirus rna genome as a means of distinguishing between rotavirus strains in order to assess its role in the evaluation of apparent hosp ...19882849635
[the immune response response in the bovine fetus to intrauterine immunization with rotaviruses]. 19882849832
epidemiology of rotavirus subgroups and serotypes in belem, brazil: a three-year study.rotavirus subgroups and serotypes were determined in 61 rotavirus-positive faecal samples obtained from children living in belém, brazil, followed up from birth to 3 years of age. fifty-five (90%) of the specimens were subgrouped and the serotypes of 30 (49%) of them were determined. subgroup ii was detected in 49 (89%) of the 55 subgrouped strains. serotype 1 was present in 15 (50%) of the 30 serotyped samples; serotypes 2, 3 and 4 were found in 30%, 3.3% and 16.7% respectively, of these specim ...19882849961
epidemiological and clinical study and electrophoretyping survey of rotavirus acute diarrhoea in a children's infectious disease unit in casablanca, morocco. 19882849963
relative frequency of human rotavirus subgroups i and ii in relation to "short" and "long" electrophoretypes of viral rna. 19882849966
a highly efficient second-step concentration technique for bacteriophages and enteric viruses using ammonium sulfate and tween 80.addition of tween 80 to a 1.5% solution of beef extract was found to enhance the elution of bacteriophages adsorbed to electronegative filters. when reconcentration of the eluate was attempted by ammonium sulfate precipitation, a floating layer containing most of the viruses was formed. this floating layer can be obtained with several nonionic detergents including tween 80 and under a salt saturation of 55% with ammonium sulfate, potassium tartrate, and sodium phosphate. virus recovery ranged fr ...19882850100
detection of animal and human enteric viruses in water from the assomption river and its tributaries.animal enteroviruses, reoviruses, and human enteric viruses were detected in water samples (20 l) from a major river system, the assomption river in the province of quebec. animal enteroviruses, probably of porcine origin (this region is a major producer of pork), were isolated on porcine cell cultures and were found in 29 to 60% of water samples from the different sites on the river and in 19 to 48% of the water samples from the tributaries. the average concentration of these animal enterovirus ...19882850104
one-year prospective cross-sectional study to assess the importance of group f adenovirus infections in children under 2 years admitted to hospital.a 1-year prospective cross-sectional study of 363 children under 2 years of age admitted to hospital was undertaken to assess the importance of group f adenovirus infections. faeces obtained within 48 hours of admission from 97 patients with and 266 patients without diarrhoea were screened by electron microscopy. viruses were identified by morphological criteria, and all adenoviruses seen were retested by immune electron microscopy to identify group f serotypes. group f adenoviruses (4 infection ...19882850345
[the significance of rotavirus and escherichia associations in diarrheal diseases in children]. 19882850529
the nucleotide sequence of the vp3 gene of porcine rotavirus osu. 19882850544
natural infection of porcine ileal dome m cells with rotavirus and enteric adenovirus. 19882850651
determination of the virus load of running waters. 19882850664
rotaviruses and their detection in environmental samples. 19882850666
human rotavirus infection enhances invasiveness of enterobacteria in ma-104 study the interaction between common pathogens causing infectious diarrhea in humans, ma 104 cell cultures were infected with human rotavirus and salmonella typhimurium or shigella flexneri or enteroinvasive escherichia coli (eiec) concomitantly. when ma-104 cells were preinfected with human rotavirus, invasiveness of s. typhimurium was significantly enhanced. the enhancement was evident after 48 h of virus preincubation. at this time virus specific antigens were demonstrated in the cell cult ...19882850823
the protection of newborn calves against experimental rotavirus infection by feeding mammary secretions from vaccinated cows.pregnant cows were given the first injection of an inactivated bovine rotavirus vaccine approximately 4 weeks before calving and a second injection just before calving. this led to the enhancement of rotavirus antibody titers in their colostrum as well as in the milk for at least 5 days after parturition. thus, when newborn calves were fed with the mammary secretions obtained from the vaccinated cows daily for 5 consecutive days they were fully refractory to experimental infection with 81/36f bo ...19882851089
the present status of rotavirus vaccine development.rotavirus infection is one of the most important causes of morbidity in young children throughout the world. the high associated mortality in southeast asia (and elsewhere) warrants the development of a vaccine. it is probable that most of the life-threatening watery diarrhoea due to rotavirus infection occurs as a result of primary infection in children aged 6-18 months after protection due to maternal antibody has diminished. thus rotavirus vaccines are targeted at young infants from birth to ...19882851184
clinical and immunological studies of rotavirus vaccines.the rit 4237 bovine rotavirus vaccine has served as a useful model for rotavirus vaccination, but the vaccine will not be further developed or tested. the main problem encountered with this vaccine was its poor "take" rate in developing countries. the reasons for this are unclear, and it is not known whether other bovine rotavirus vaccines are more efficacious in this respect. wc-3 bovine rotavirus vaccine will shortly be tested at several sites in developing countries. the rhesus rotavirus vacc ...19882851185
[a solid-phase coagglutination reaction for detecting igm antibodies in rotavirus infection]. 19882851224
[molecular epidemiology of rotavirus infections at units of children's hospitals].from the 16.6. 1983 until the 5.9. 1984 337 stool specimens were collected from 258 patients in the pediatric clinics of the university hospital of mannheim and of heidelberg. they were tested for rotavirus antigen and rotavirus genome-dsrna. of the 192 rotavirus antigen-positive children, 91.1% experienced the infection in their first year of life with a distinct peak during the winter and a noticeably high percentage of newborn babies being affected. forty-six electropherotypes and three of th ...19882851237
[data on the study of the epidemiology of rotavirus infection in leningrad].the results obtained in the study of rotavirus infection in leningrad in 1984-1987 are presented. enzyme immunoassay techniques were used for the examination of 4,715 children aged 0-14 years and 1,162 adults with diagnosed acute enteral infection of unknown etiology, as well as the control group of 556 of healthy children aged 0-6 years and 77 healthy adults. the rotavirus antigen was detected in 1.210 sick children (25.7%) and 133 sick adults (11.4%), as well as in 6 healthy children (1.1%), b ...19882851247
[carrier state of enteric viruses in ivory coast pregnant women in good health].the present work emphasize the great diversity of the viral species encountered in pregnant women (rotavirus, coronavirus, adenovirus, poliovirus, coxsackievirus). in each of the different regions studied, one or two viral types are usually predominant. in man (west ivory coast) region for instance, rotaviruses and coronaviruses were mainly encountered; in bouaké, polioviruses and adenoviruses were predominant whereas in bondoukou and odienné respectively coxsackieviruses and adenoviruses were p ...19882851394
distribution of rotavirus antigen in intestinal lymphoid tissues: potential role in development of the mucosal immune response to rotavirus.groups of suckling balb/c mice were inoculated with murine rotavirus (mrv) at 5 days of age and sequentially tested for the presence of mrv antigen (ag) in intestinal villus epithelium (ve), peyer's patch (pp), mesenteric lymph node (mln), spleen, liver and lung, using indirect immunofluorescence techniques (if). mrv ag was first detected in ve 24 h after oral administration of the virus and remained in ve for as long as 10 days post-inoculation (pi). mrv ag was observed in the epithelium overly ...19882851396
synthesis of the major inner capsid protein vp6 of the human rotavirus wa in escherichia coli.the gene for the major inner capsid protein vp6 of human rotavirus strain wa has been cloned and placed into a bacterial expression vector under the control of the inducible hybrid trp-lac (tac) promoter. recombinant vp6 was produced at low levels in a cell-free escherichia coli transcription-translation system programmed with this expression plasmid. the yield of vp6 synthesized in the extract could be increased several-fold by introduction of point mutations upstream and downstream from the st ...19882851499
rotavirus-like infection associated with diarrhea in okapi. 19862851576
development of an enzyme immunoassay for the hawaii agent of viral gastroenteritis.the hawaii agent is a norwalk-like virus of acute gastroenteritis in humans which is antigenically distinct from the prototype norwalk agent. we established a solid phase sandwich type microtiter enzyme immunoassay (eia) for hawaii antigen employing sera and stools from experimentally challenged volunteers as reagents. this assay detected the hawaii agent in stools from 3 of 8 volunteers who were ill after oral challenge with the hawaii agent, including one specimen which was positive to a dilut ...19882851600
a rapid and sensitive solution hybridisation assay for the quantitative determination of specific viral rna sequences.a solution hybridisation assay has been developed which allows the quantitation of specific viral (rna) sequences in infected cells. the assay makes use of single-stranded (ss) rna probes of known polarity synthesised at high specific activity in vitro from cdna clones of the relevant viral gene by the sp6 or t7 rna polymerase. these probes are used together with samples containing the rna to be detected at a known concentration to construct a calibration curve to relate rnase resistant radioact ...19882851603
human serogroup c rotavirus in finland.among 600 rotavirus specimens collected in finland during 1985-1987, 6 isolates were found to be related to serogroup c rotavirus by rna profile analysis and solid phase immune electron microscopy (spiem). the group c isolates originated from 4 children (4.5, 4.5, 6.5 and 7 years old) with diarrhoea.19882851876
rotaviruses in infants with diarrhea studied by viral rna electrophoresis in ankara, turkey.incidence of rotavirus in children under 2 years of age, admitted to 2 children's hospitals i n ankara, turkey, from july 1984-june 1985 was measured using viral rna electrophoresis in agarose gels. 375 children were selected at random from over 14,000 diarrhea admissions, and their stool specimens taken on admission were compared to those of 333 children without diarrhea. 61 (16.3%) of the diarrhea cases had detectable rotavirus, compared to 0% in controls. bacteriologic tests for salmonella ...19872851886
[an outbreak of acute rotavirus gastroenteritis in a day care center]. 19882852154
[etiological investigation of an epidemic adult acute diarrhea outbreak in yangi]. 19882852542
occurrence of changes in human rotavirus serotypes with concurrent changes in genomic rna investigate the serotypic and genetic diversity of human rotavirus strains, we have tested 513 and 519 fecal rotavirus specimens, respectively, by an enzyme-linked immunosorbent assay with serotype-specific monoclonal antibodies and by polyacrylamide gel electrophoresis of the segmented rna genome. of the 513 specimens, 375 were typed as serotype 1 (47.3%), serotype 2 (2.9%), serotype 3 (2.9%), or serotype 4 (17.7%). in addition, a presumptive new human serotype, tentatively referred to as se ...19882852673
clinical features of acute infantile gastroenteritis associated with human rotavirus subgroups i and ii.during a prospective 3-year study, clinical and epidemiological features of rotavirus gastroenteritis in black infants were investigated. fever and temperatures exceeding 39 degrees c were more frequent in children shedding subgroup ii rotaviruses, whereas vomiting was more pronounced in children with subgroup i infection. diarrhea, dehydration, duration of illness, and the need for admission to a hospital ward were similar in both groups.19882852676
reactogenicity and antigenicity of rhesus rotavirus vaccine (mmu-18006) in newborn infants in venezuela.the reactogenicity and antigenicity of the rhesus rotavirus vaccine, strain mmu18006, developed at the laboratory of infectious diseases (national institute of allergy and infectious diseases, national institutes of health) were examined in a double blind, placebo-controlled study of 40 newborn infants in caracas, venezuela. the children were observed for the first few days after birth in the hospital nursery and by home visits for 10 days after vaccination to detect any adverse reactions. no re ...19882852792
nosocomial infection by rotavirus and adenovirus within one month. 19882852886
rotavirus and adult diarrhea. 19882852892
rotavirus epidemiology in vellore, south india: group, subgroup, serotype, and electrophoretype.rotaviruses were detected in 163 of 916 (17.8%) specimens collected from children under 3 years of age with gastroenteritis in vellore, south india, between august 1983 and july 1985. rotaviruses were detected throughout the study period, with a peak prevalence in december to february (winter) and june to august (southwest monsoon season). a total of 117 rotavirus strains were tested for subgroup, serotype, and rotavirus double-stranded rna electrophoretic migration pattern; 24.8% of the strains ...19882853177
evaluation of abbott testpack rotavirus with clinical specimens.testpack rotavirus was compared with the pathfinder rotavirus assay for the detection of rotaviral antigens in feces from 137 patients. the symptomatology and ages of the patients varied from no symptoms to watery diarrhea and from less than 3 months to greater than 60 years of age. the sensitivity and specificity, before resolution, were 89.2 and 90.0%, respectively. after resolution with a blocking reagent, the results were 100% sensitivity and 98.9% specificity. the performance was similar fo ...19882853178
rotavirus infection of thai infants: antigen detection, rna electrophoresis and virus cultivation. 19872853186
rapid detection of rotavirus in faeces by a slide latex agglutination test as compared with an enzyme-linked immunosorbent assay. 19872853187
[diagnosis of viral pathogens in intestinal infections--results from hospitalized children]. 19882853320
[mechanisms of virus-induced membrane and cell fusion reactions]. 19882853482
comparison of rotavirus infection in newborn infants and hospitalized older children by electrophoresis of genome rna. 19882853505
evaluation of a new enzyme immunoassay (testpack rotavirus) for the detection of rotavirus in fecal hundred and forty fecal specimens were tested for rotavirus using two immunoassays, testpack rotavirus and pathfinder rotavirus. five discordant specimens were evaluated by a blocking assay. the sensitivity, specificity, positive predictive value, and negative predictive value were 98, 100, 100, and 99% for testpack and 92, 100, 100, and 96% for pathfinder, respectively.19882853661
comparison of a protein-stabilized rotazyme ii test, with standard rotazyme ii, and electron microscopy for detection of rotavirus.we compared the performance of rotazyme ii with protein-stabilized diluent to standard rotazyme ii and to direct electron microscopy, for the detection of rotavirus. sensitivity and specificity were: standard rotazyme ii, 79 and 100%, respectively; rotazyme ii with protein stabilizers, both 100%, and electron microscopy, 75 and 100%, respectively. commercial availability of this product should improve rotavirus detection for laboratories utilizing rotazyme ii methodology.19882853662
human rotavirus detection in stool specimens using enzyme-linked immunosorbent assays and latex agglutination test.a total of 194 stool samples from sick and healthy children were tested by three commercial tests: two rotavirus enzyme-linked immunosorbent assays (elisa) and one latex agglutination test (ltx). the concordance, sensibility and specificity of these tests were calculated. the results suggest to consider as positive the samples reactive to two enzyme immunoassays at least. the latex test, although less sensitive than enzygnost-rotavirus and rotazyme ii, was found to be especially useful for the r ...19882853953
[immunobiologic properties of swine rotavirus].immunobiological properties of swine rotaviruses were studied. a field isolate of this virus was markedly virulent for newborn colostrum-less piglets. an attenuated strain of swine rotavirus was obtained by serial passages in continuous cell cultures (ma-104 and spev). the virus of the 60th passage was nonvirulent for colostrum-less piglets although produced marked seroconversion. naso-oral immunization of piglets with the attenuated strain prevented the manifestation of clinical disease upon ch ...19882854679
[isolation of immunoglobulins from the egg yolk of hens immunized with intestinal viruses].antibodies from the yolk of eggs of chicken immunized with enteric viruses (simian rotavirus sa 11, poliomyelitis virus type 2, and coxsackie b2) were obtained. the time course of the emergence and amplification of antibody in the yolk was followed for several months by neutralization tests and enzyme immunoassay. the long-term persistence of a high antibody level in the yolk, the simplicity of generation of large amounts of chromatographically pure preparations, as well as specificity of chicke ...19882854680
[rotavirus study by electron microscopy and polyacrylamide gel electrophoresis of rna]. 19882854681
[comparative evaluation of methods for diagnosing rotavirus gastroenteritis]. 19882854682
long-term follow-up of infants from birth for rotavirus antigen and antibody in the feces. 19882854700
comparison of two methods for detection of transmissible gastroenteritis virus in feces of pigs with experimentally induced indirect, double-antibody sandwich-type elisa for detection of transmissible gastroenteritis virus (tgev) was developed, using a solid phase of rabbit hyperimmune serum and a pool of 3 antipeplomer monoclonal antibodies to trap and to detect the virus, respectively. the technique was used to detect viral antigen in feces of pigs that had been infected with the virulent miller strain, the attenuated purdue strain, or the erica strain (a dutch field isolate) of tgev. the results were compared w ...19882854707
[molecular epidemiology of rotavirus infection as revealed by rna polyacrylamide gel electrophoresis: analysis of epidemics during 1981-1983]. 19882854861
[bacterial, rotavirus and mixed etiologies of diarrhea in children]. 19882854908
immunofluorescent antibody to rotavirus and antigen excretion in infantile acute diarrhoea. 19882854909
[the inactivation of viruses in sewage sludge treatment]. 19882854912
rapid laboratory detection of rotavirus in faeces from children with acute enteritis. 19882855228
prevalence of bacterial pathogens and rotavirus in hospitalised children with acute diarrhoea in delhi, india. 19882855229
Displaying items 1901 - 2000 of 10398