| whitefly population dynamics and evaluation of whitefly-transmitted tomato yellow leaf curl virus (tylcv)-resistant tomato genotypes as whitefly and tylcv reservoirs. | sweetpotato whitefly, bemisia tabaci (gennadius), and whitefly-transmitted tomato yellow leaf curl virus (tylcv) are major threats to tomato production in the southeastern united states. tylcv was introduced to florida from the caribbean islands and has spread to other southern states of the united states. in georgia, in recent years, the incidence of tylcv has been steadily increasing. studies were conducted to monitor population dynamics of whiteflies in the vegetable production belt of georgi ... | 2012 | 22928328 |
| a bead-based suspension array for the multiplexed detection of begomoviruses and their whitefly vectors. | bead-based suspension array systems enable simultaneous fluorescence-based identification of multiple nucleic acid targets in a single reaction. this study describes the development of a novel approach to plant virus and vector diagnostics, a multiplexed 7-plex array that comprises a hierarchical set of assays for the simultaneous detection of begomoviruses and bemisia tabaci, from both plant and whitefly samples. the multiplexed array incorporates genus, species and strain-specific assays, offe ... | 2014 | 24388931 |
| lamium amplexicaule (lamiaceae): a weed reservoir for tomato yellow leaf curl virus (tylcv) in korea. | after the first identification of tomato yellow leaf curl virus (tylcv) in the southern part of korea in 2008, tylcv has rapidly spread to tomato farms in most regions of korea. from 2008 to 2010, a survey of natural weed hosts that could be reservoirs of tylcv was performed in major tomato production areas of korea. about 530 samples were collected and identified as belonging to 25 species from 11 families. pcr and southern hybridization were used to detect tylcv in samples, and replicating for ... | 2014 | 24327090 |
| molecular variability and evolution of a natural population of tomato yellow leaf curl virus in shanghai, china. | tomato yellow leaf curl virus (tylcv), belonging to the genus begomovirus of the family geminiviridae, is emerging as the most destructive pathogen of tomato plants. since the first report of tylcv in shanghai, china in 2006, tylcv has spread rapidly to 13 provinces or autonomous regions of china. in this study, the molecular variability and evolution of tylcv were monitored in shanghai from its first upsurge in 2006 until 2010. full-length genomic sequences of 26 isolates were obtained by rolli ... | 2014 | 24510706 |
| a review of the mechanisms and components that determine the transmission efficiency of tomato yellow leaf curl virus (geminiviridae; begomovirus) by its whitefly vector. | begomoviruses are a group of icosahedral single stranded dna viruses exclusively transmitted by the sweet potato whitefly bemisia tabaci in a persistent, circulative manner. in this mode of transmission, begomoviruses are acquired by their insect vector as intact virions from the plant phloem, move along the food canal, foregut and esophagus and reach the midgut where they are absorbed into the hemolymph via the filter chamber. the filter chamber is the site where most of the ingested food is fi ... | 2014 | 24508344 |
| expression and functional characterisation of a soluble form of tomato yellow leaf curl virus coat protein. | tomato yellow leaf curl virus (tylcv), a member of the genus begomovirus within the family geminiviridae, is an important pathogen of tomato in many tropical, subtropical and temperate regions. tylcv is exclusively transmitted by the whitefly bemisia tabaci in a circulative manner. the viral coat protein (cp) has been assumed to play important roles in the entry of tylcv into the insect midgut cells. | 2014 | 24488592 |
| degradation mechanisms of the tomato yellow leaf curl virus coat protein following inoculation of tomato plants by the whitefly bemisia tabaci. | tomato yellow leaf curl virus (tylcv) is a begomovirus infecting tomato cultures worldwide. tylcv is transmitted to plants by the whitefly bemisia tabaci. once in the plant, the virus is subjected to attack by the host-plant defences, which may include sequestration in aggregates, proteolysis, ubiquitination, 26s proteasome degradation and autophagy. elucidating how the virus avoids destruction will make it possible to understand infection and possibly devise countermeasures. | 2014 | 24464776 |
| no evidence of seed transmissibility of tomato yellow leaf curl virus in nicotiana benthamiana. | recent work reported that tomato yellow leaf curl virus (tylcv) is seed-transmissible in tomato, contrary to previous belief. in this work, we explore whether tylcv is also a seed-borne virus in another member of the solanaceae family, the experimental host nicotiana benthamiana. | 2017 | 28471116 |
| insect symbiont facilitates vector acquisition, retention, and transmission of plant virus. | tomato yellow leaf curl virus (tylcv) was first detected in china in 2006, following the introduction of bemisia tabaci q into china in 2003. since then, the incidence of tylcv in tomato fields in china has greatly increased as has the abundance and distribution of q whiteflies containing the bacterial symbiont hamiltonella with high frequency. this suggested that the symbiont hamiltonella might associate with the transmission efficiency of tylcv by the whitefly vector. here we report the first ... | 2013 | 23455639 |
| involvement of the hexose transporter gene leht1 and of sugars in resistance of tomato to tomato yellow leaf curl virus. | | 2013 | 23430051 |
| multiple forms of vector manipulation by a plant-infecting virus: bemisia tabaci and tomato yellow leaf curl virus. | for many insect-vectored plant viruses, the relationship between feeding behavior and vector competence may prove integral to an understanding of the epidemiology of the resulting plant disease. while plant-infecting viruses are well known to change host plant physiology in a way that makes them more attractive to vectors, viral manipulation of the vectors themselves has only recently been reported. previous research suggested that the rapid spread of tomato yellow leaf curl virus (tylcv) throug ... | 2013 | 23408638 |
| accurate and sensitive diagnosis of geminiviruses through enrichment, high-throughput sequencing and automated sequence identification. | existing diagnostic techniques used to identify plant-infecting dna viruses and their associated molecules are often limited in their specificity and can be challenged by samples containing multiple viruses. we adapted a simple method of amplifying circular viral dna and, in combination with high-throughput sequencing and bioinformatic analysis, used it as a virus diagnostic method. we validated this diagnostic method with a plant sample infected with a tomato yellow leaf curl geminivirus infect ... | 2012 | 22327393 |
| molecular identification of a new begomovirus associated with mosaic disease of jatropha curcas l. in nigeria. | we report the complete nucleotide sequence of dna-a of a begomovirus naturally infecting jatropha curcas l. in nigeria. symptoms observed on infected plants were severe mosaic, mottling and blistering of leaves. the virus, which we provisionally name "jatropha mosaic nigeria virus" (jmnv), has a monopartite genome of 2,779 to 2,789 nucleotides. pairwise comparisons of dna-a sequences showed that jmnv had maximum nucleotide sequence identity (72%) with a strain of tomato yellow leaf curl virus. s ... | 2013 | 23074041 |
| mapping and introgression of a tomato yellow leaf curl virus tolerance gene, ty-1. | the whitefly-transmitted tomato yellow-leaf curl gemini-virus (tylcv) is a major pathogen of tomatoes. the wild tomato species lycopersicon chilense, which is resistant to the virus, was crossed to the cultivated tomato, l. esculentum. the backcross-1 selfed (bc1s1) generation was inoculated and a symptomless plant was selected. this plant was analyzed using 61 molecular markers, which span the tomato genome, to determine which l. chilense chromosome segments were introgressed. a bc2s1 populatio ... | 1994 | 24185918 |
| inhibition of binding of tomato yellow leaf curl virus rep to its replication origin by artificial zinc-finger protein. | previously we demonstrated that inhibition of replication-associated protein (rep) binding to its replication origin by artificial zinc-finger proteins (azps) is a powerful method to prevent plant virus infection in vivo. in the present study, we applied the azp technology to tomato yellow leaf curl virus (tylcv), which is a limiting factor in tomato cultivation worldwide. first, we determined 5'-atcggtgt atcggtgt-3' in the 195-bp intergenic region of the tylcv-israel strain, a strain reported f ... | 2013 | 22576255 |
| vector development and vitellogenin determine the transovarial transmission of begomoviruses. | the majority of plant viruses are transmitted by insect vectors between hosts, and transovarial transmission of viruses from vector parents to offspring has great significance to their epidemiology. begomoviruses are transmitted by the whitefly bemisia tabaci in a circulative manner and are maintained through a plant-insect-plant cycle. other routes of begomovirus transmission are not clearly known. here, we report that transovarial transmission from female whiteflies to offspring often happens ... | 2017 | 28607073 |
| towards area wide management of insect vectored viruses of tomatoes in the bowen district. | the bowen region of northern queensland is an important winter production area for tomatoes in australia. there are three economically important viruses in the region that affect tomato, tomato yellow leaf curl virus (tylcv), tomato spotted wilt virus (tswv) and potato leafroll virus (plrv), which are vectored by whiteflies, thrips and aphids, respectively. an area wide management approach is required to lower the primary inoculum throughout the district. to this end, we undertook investigations ... | 2017 | 28625668 |
| facultative symbiont hamiltonella confers benefits to bemisia tabaci (hemiptera: aleyrodidae), an invasive agricultural pest worldwide. | bacterial symbionts infect most insect species, including important pests such as whitefly, bemisia tabaci (gennadius) (hemiptera: aleyrodidae), and often exert important effects on host ecology. the facultative symbiont hamiltonella is found at high frequencies in the b. tabaci med (type: mediterranean-med) in china. the prevalence of this symbiont in natural populations suggests beneficial effects of infection or manipulation of host reproduction. to date, however, no empirical studies on the ... | 2013 | 24280594 |
| complete genome sequence of a new begomovirus associated with yellow mosaic disease of hemidesmus indicus in india. | the complete dna a genome of a virus isolate associated with yellow mosaic disease of a medicinal plant, hemidesmus indicus, from india was cloned and sequenced. the length of dna a was 2825 nucleotides, 35 nucleotides longer than the unit genome of monopartite begomoviruses. comparison of the nucleotide sequence of dna a of the virus isolate with those of other begomoviruses showed maximum sequence identity of 69 % to dna a of ageratum yellow vein china virus (ayvcnv; aj558120) and 68 % with to ... | 2014 | 24276235 |
| v2 of tomato yellow leaf curl virus can suppress methylation-mediated transcriptional gene silencing in plants. | tomato yellow leaf curl virus (tylcv) is a dna virus belonging to the genus begomovirus. tylcv replicates using double-stranded dna intermediates that can become the target of plant transcriptional gene silencing (tgs). here, we show that the v2 protein of tylcv can suppress tgs of a transcriptionally silenced green fluorescent protein (gfp) transgene in nicotiana benthamiana line 16-tgs. through bisulfite sequencing and chop-pcr, we demonstrated that the tylcv v2 can reverse gfp transgene silen ... | 2014 | 24187017 |
| rapid spread of tomato yellow leaf curl virus in china is aided differentially by two invasive whiteflies. | tomato yellow leaf curl virus (tylcv) was introduced into china in 2006, approximately 10 years after the introduction of an invasive whitefly, bemisia tabaci (genn.) b biotype. even so the distribution and prevalence of tylcv remained limited, and the economic damage was minimal. following the introduction of q biotype into china in 2003, the prevalence and spread of tylcv started to accelerate. this has lead to the hypothesis that the two biotypes might not be equally competent vectors of tylc ... | 2012 | 22514670 |
| a developmentally regulated lipocalin-like gene is overexpressed in tomato yellow leaf curl virus-resistant tomato plants upon virus inoculation, and its silencing abolishes resistance. | to discover genes involved in tomato resistance to tomato yellow leaf curl virus (tylcv), we previously compared cdna libraries from susceptible (s) and resistant (r) tomato lines. among the genes preferentially expressed in r plants and upregulated by tylcv infection was a gene encoding a lipocalin-like protein. this gene was termed solanum lycopersicum virus resistant/susceptible lipocalin (slvrslip). the slvrslip structural gene sequence of r and s plants was identical. slvrslip was expressed ... | 2012 | 22843056 |
| upregulation of temperature susceptibility in bemisia tabaci upon acquisition of tomato yellow leaf curl virus (tylcv). | acquisition of plant viruses has various effects on physiological mechanisms in vector insects. bemisia tabaci is the only known vector of tomato yellow leaf curl virus (tylcv), which is a serious virus affecting tomato cultivars. in this study, the lifespan of q1 biotype was compared between non-viruliferous (nv) and tylcv-viruliferous (v) whiteflies. total lifespan from egg to adult death of nv whiteflies was 62.54 days but 10.64 days shorter in v whiteflies. we investigated the temperature su ... | 2012 | 22841829 |
| detection and molecular characterization of tomato yellow leaf curl virus naturally infecting lycopersicon esculentum in egypt. | tomato yellow leaf curl virus (tylcv) infections of tomato crops in egypt were widely spread in 2014. infected symptomatic tomato plants from different governorates were sampled. tylcv strains israel and mild (tylcv-il, tylcv-mild) were identified by multiplex and real-time pcr. in addition, nucleotide sequence analysis of the v1 and v2 protein genes, revealed ten tylcv egyptian isolates (tylcv from ty1 to 10). phylogenetic analysis showed their high degree of relatedness with tylcv-il jordan is ... | 2017 | 28854789 |
| seed transmission of tomato yellow leaf curl virus in white soybean (glycine max). | tomato yellow leaf curl virus (tylcv) infection of the common bean (phaseolus vulgaris) has been reported, but soybean (glycine max) has not previously been identified as a tylcv host. five cultivars of white soybean were agro-inoculated using an infectious tylcv clone. at 30 days post-inoculation, they showed infection rates of 25% to 100%. typical tylcv symptoms were not observed in any inoculated plants. to examine whether tylcv was transmitted in soybean seeds, dna was isolated from bundles ... | 2017 | 28811759 |
| expression of tomato yellow leaf curl virus coat protein using baculovirus expression system and evaluation of its utility as a viral antigen. | dna encoding the coat protein (cp) of an egyptian isolate of tomato yellow leaf curl virus (tylcv) was inserted into the genome of autographa californica nucleopolyhedrovirus (acnpv) under the control of polyhedrin promoter. the generated recombinant baculovirus construct harboring the coat protein gene was characterized using pcr analysis. the recombinant coat protein expressed in infected insect cells was used as a coating antigen in an indirect enzyme-linked immunosorbent assay (elisa) and do ... | 2017 | 28794924 |
| lower incidence and severity of tomato virus in elevated co(2) is accompanied by modulated plant induced defence in tomato. | elevation in atmospheric co(2) concentration broadly affects plant phenology and physiology, and these effects may alter the performance of plant viruses. the effects of elevated co(2) on the susceptibility of tomato plants to tomato yellow leaf curl virus (tylcv) were examined for two successive years in open top chambers (otc) in the field. we experimentally tested the hypothesis that elevated co(2) would reduce the incidence and severity of tylcv on tomato by altering plant defence strategies ... | 2012 | 22512888 |
| acylsucrose-producing tomato plants forces bemisia tabaci to shift its preferred settling and feeding site. | the whitefly bemisia tabaci (genn.) causes dramatic damage to plants by transmitting yield-limiting virus diseases. previous studies proved that the tomato breeding line abl 14-8 was resistant to b. tabaci, the vector of tomato yellow leaf curl disease (tylcd). this resistance is based on the presence of type iv glandular trichomes and acylsucrose production. these trichomes deter settling and probing of b. tabaci in abl 14-8, which reduces primary and secondary spread of tylcd. | 2012 | 22427950 |
| [molecular detection of tomato yellow leaf curl virus (tylcv)]. | tomato yellow leaf curl virus (tylcv) is currently considered as one of the most devastating viruses in cultivated tomatoes (solanum lycopersicum) worldwide. we reported here the development of a pcr-based method to quickly detect tylcv using the primer pairs (tylcv-f: 5'-acg cat gcc tct aat cca gtg ta-3' and tylcv-r: 5'-cca ata agg cgt aag cgt gta gac-3'), which was designed based on the genome sequence of tylcv. a tylcv-specific band of 543 bp was amplified from infected tomato plants. this pr ... | 2012 | 22425956 |
| genome-wide identification of dicer-like, argonaute and rna-dependent rna polymerase gene families and their expression analyses in response to viral infection and abiotic stresses in solanum lycopersicum. | dicer, argonaute and rna-dependent rna polymerase form the core components to trigger rna silencing. although tomato (solanum lycopersicum) is a dicotyledon model plant, no systematic analysis and expression profiling of these genes in tomato has been undertaken previously. in this study, seven dicer-like (sldcls), 15 argonaute (slagos) and six rna-dependent rna polymerase (slrdrs) genes were identified in tomato. these genes were categorized into four subgroups based on phylogenetic analyses. c ... | 2012 | 22406496 |
| biological invasions of geminiviruses: case study of tylcv and bemisia tabaci in reunion island. | in the last 20 years, molecular ecology approaches have proven to be extremely useful to identify and assess factors associated with viral emerging diseases, particularly in economically and socially important tropical crops such as maize (maize streak disease) and cassava (cassava mosaic disease). molecular ecology approaches were applied in reunion island to analyze the epidemic of tomato yellow leaf curl disease, which has been affecting the island since the end of the 1990s. before the invas ... | 2012 | 23235470 |
| progressive aggregation of tomato yellow leaf curl virus coat protein in systemically infected tomato plants, susceptible and resistant to the virus. | tomato yellow leaf curl virus (tylcv) coat protein (cp) accumulated in tomato leaves during infection. the cp was immuno-detected in the phloem associated cells. at the early stages of infection, punctate signals were detected in the cytoplasm, while in the later stages aggregates of increasing size were localized in cytoplasm and nuclei. sedimentation of protein extracts through sucrose gradients confirmed that progress of infection was accompanied by the formation of cp aggregates of increasin ... | 2013 | 23099086 |