| production of human vitronectin in nicotiana benthamiana using the inpact hyperexpression platform. | human vitronectin (hvn) is a glycoprotein that functions as a cell adhesion molecule and a regulator of coagulation in blood plasma and the extracellular matrix. in vitro, hvn is added to serum-free media in order to promote the adhesion of animal cells to tissue culture surfaces and the proliferation of undifferentiated stem cells. here, we report the production of hvn in nicotiana benthamiana using the inducible in plant activation (inpact) hyperexpression platform. n. benthamiana plants were ... | 2017 | 28640945 |
| two novel mastreviruses from chickpea (cicer arietinum) in australia. | two novel mastreviruses (genus mastrevirus; family geminiviridae), with proposed names chickpea chlorosis virus (cpcv) and chickpea redleaf virus, are described from chickpea (cicer arietinum) from eastern australia. the viruses have genomes of 2,582 and 2,605 nucleotides, respectively, and share similar features and organisation with typical dicot-infecting mastreviruses. two distinct strains of cpcv were suggested by phylogenetic analysis. additionally, a partial mastrevirus rep sequence from ... | 2010 | 20734091 |
| two dicot-infecting mastreviruses (family geminiviridae) occur in pakistan. | most mastreviruses (family geminiviridae) infect monocotyledonous hosts and are transmitted by leafhopper vectors. only two mastrevirus species, tobacco yellow dwarf virus from australia and bean yellow dwarf virus (beydv) from south africa, have been identified whose members infect dicotyledonous plants. we have identified two distinct mastreviruses in chickpea stunt disease (csd)-affected chickpea originating from pakistan. the first is an isolate of beydv, previously only known to occur in so ... | 2008 | 18566736 |
| molecular characterization of a subgroup i geminivirus from a legume in south africa. | a south african geminivirus for which we propose the name bean yellow dwarf virus (beydv) has been isolated from french bean (phaseolus vulgaris cv. bonus) showing stunting, chlorosis and leaf curl symptoms. a full-length cloned copy of the viral genome produced characteristic symptoms of the disease when reintroduced into french bean by agroinoculation, and was systemically infectious in nicotiana benthamiana, n. tabacum, lycopersicon esculentum, datura stramonium and arabidopsis thaliana. beyd ... | 1997 | 9267015 |
| classification and identification of geminiviruses using sequence comparisons. | the genomes and orfs of 36 geminiviruses were compared to obtain phylogenetic trees and frequency distributions of all possible pairwise comparisons with an objective to classify geminiviruses. such comparisons show that geminiviruses form two distinct clusters of leafhopper-transmitted viruses that infect monocots (subgroup i) and whitefly-transmitted viruses that infect dicots (subgroup iii), irrespective of the part of the genome considered. of the two leafhopper-transmitted viruses that infe ... | 1995 | 7844548 |
| the nucleotide sequence of the infectious cloned dna component of tobacco yellow dwarf virus reveals features of geminiviruses infecting monocotyledonous plants. | an infectious clone of the australian geminivirus tobacco yellow dwarf virus (tobydv) was constructed from virus-specific double-stranded dna isolated from infected tobacco and used to demonstrate a single-component genome. the nucleotide sequence of tobydv dna comprises 2580 nucleotides. tobydv dna has three coding regions, two in the virion sense and one in the complementary sense, homologous to those identified for other geminiviruses, particularly those infecting monocotyledonous (monocot) p ... | 1992 | 1546458 |