| a nosocomial outbreak of parvovirus b19 infection. | | 1992 | 1284951 |
| diagnosis of human parvovirus b19 infections by polymerase chain reaction. | the polymerase chain reaction (pcr) was used for detecting parvovirus b19 dna in clinical specimens. a pair of oligonucleotide primers spanning the psti-fragment of the b19 virus genome was used for pcr, and a pcr product of 727 bp was amplified. b19 virus dna was detected in all sera (n = 26) of individuals in the incubation period and acute phase of infection. pcr was useful for detecting viral b19 dna in amniotic fluid and fetal blood of hydropic fetuses, confirming fetal b19 virus infection. | 1992 | 1287802 |
| congenital infections and the nervous system. | despite vaccines, new antimicrobials, and improved hygienic practices, congenital infections remain an important cause of death and long-term neurologic morbidity among infants world-wide. important agents include toxoplasma gondii, cytomegalovirus, treponema pallidum, herpes simplex virus types 1 and 2, and rubella virus. in addition, several other agents, such as the varicella zoster virus, human parvovirus b19, and borrelia burgdorferi, can potentially infect the fetus and cause adverse fetal ... | 1992 | 1321971 |
| [infections caused by human parvovirus b19]. | | 1992 | 1322711 |
| [maternal viral infections as a fetal risk factor]. | after a review of the literature reports from recent years viral infections in pregnant women are presented as a fetal and neonatal risk factor. maternal infections with rubella virus, cytomegalovirus++, hepatitis, coxsackie b, varicella, herpes, poliomyelitis, parvovirus b19 and hiv are discussed stressing their unfavourable effect on the developing embryo, fetus or newborn. | 1992 | 1336911 |
| aplastic crisis due to human parvovirus b19 infection in hereditary hemolytic anaemia. | specific anti-b19 igm was demonstrated in sera from three children showing transient aplastic crisis. a two years-old boy living in rio de janeiro suffering from sickle-cell anaemia showed the crisis during august, 1990. two siblings living in santa maria, rs, developed aplastic crisis during may, 1991, when they were also diagnosed for hereditary spherocytosis. for a third child from this same family, who first developed aplastic crisis no igm anti-b19 was detected in her sera. | 1992 | 1342114 |
| polyarteritis nodosa and parvovirus b19 infection. | | 1992 | 1346840 |
| human parvovirus b19 in clotting factor concentrates: b19 dna detection by the nested polymerase chain reaction. | the presence of b19 parvovirus in plasma from blood donors is seldom demonstrable, but clotting factor concentrates, prepared from large plasma pools, may be able to transmit b19 virus infection, and the effectiveness of different chemical and physical treatment to inactivate this virus is not yet known. in this study we report on the detection of b19 dna in 25 clotting factor concentrates, prepared by a variety of procedures of purification and inactivation; dot blot hybridization and southern ... | 1992 | 1390215 |
| a block in full-length transcript maturation in cells nonpermissive for b19 parvovirus. | vertebrate parvoviruses share a similar genomic organization, with the capsid proteins encoded by genes on the right side and nonstructural proteins encoded by genes on the left side. the temporal and cell-specific appearances of these two types of gene products are regulated by a variety of genetic mechanisms. rodent parvovirus structural proteins, for example, are encoded by a separate promoter which is positively regulated by nonstructural-gene products. in contrast, for the human b19 parvovi ... | 1992 | 1385833 |
| unique region of the minor capsid protein of human parvovirus b19 is exposed on the virion surface. | capsids of the b19 parvovirus are composed of major (vp2; 58 kd) and minor (vp1; 83 kd) structural proteins. these proteins are identical except for a unique 226 amino acid region at the amino terminus of vp1. previous immunization studies with recombinant empty capsids have demonstrated that the presence of vp1 was required to elicit virus-neutralizing antibody activity. however, to date, neutralizing epitopes have been identified only on vp2. crystallographic studies of a related parvovirus (c ... | 1992 | 1376332 |
| a combined pseudoreplica-immunochemical technique for research and diagnostic virology. | pseudoreplica and immunochemical techniques were combined in a single protocol for identification of virus in research and clinical specimens. stock preparations of vesicular stomatitis virus (vsv), cytomegalovirus (cmv), and herpes simplex virus (hsv) were used to develop the technique. traditional pseudoreplicas of viral stock solutions were prepared but not negatively stained. the virus was then immunolabeled in two stages. virus-specific polyclonal antisera were used in the first stage; coll ... | 1992 | 1375519 |
| morphological diagnosis of parvovirus b19 infection. a cytopathic effect easily recognized in air-dried, formalin-fixed bone marrow smears stained with hematoxylin-eosin or wright-giemsa. | readily identifiable intranuclear inclusions characterize parvovirus b19 cytopathic effect in formalin-fixed, paraffin-embedded material. such inclusions are not apparent in air-dried smears of bone marrow aspirates. brief formalin fixation of bone marrow smears, followed by either hematoxylin-eosin or wright-giemsa staining, permitted easy detection of parvovirus b19 inclusions in material from a renal transplant recipient with parvovirus b19 infection documented serologically and by electron m ... | 1992 | 1370757 |
| parvovirus-b19-related pancytopenia in children with hiv infection. | | 1992 | 1351981 |
| viral-associated haemophagocytosis with parvovirus-b19-related pancytopenia. | viral-associated haemophagocyte syndrome in response to infection with human parvovirus b19 was seen in 2 patients with hereditary spherocytosis. depressed reticulocyte response during acute parvovirus infection is a known cause of hypoproliferative crises in patients with reduced erythrocyte lifespan; the observation of parvovirus-associated haemophagocytosis could account for the pancytopenia that may accompany human parvovirus b19 infection. | 1992 | 1349370 |
| parvovirus b19 outbreak in a children's ward. | parvovirus b19 infection can cause severe complications in pregnant women, individuals with haemolytic anaemia, and those who are immunocompromised. in a hospital outbreak of this infection, a balance should be struck between protection of these individuals and the maintenance of medical services. the index case of an outbreak of parvovirus b19 infection among staff and patients of a paediatric ward was not identified. 58 members of staff were screened for b19 markers and 4 of the 6 susceptible ... | 1992 | 1345828 |
| human b19 parvovirus infection: an example of multiple pathogenic effects determined by differences in host susceptibility. | b19 infection offers some general lessons about human viruses and their possible effects on the human host, as follows: (1) ubiquitous apparently benign viruses may have severe effects on a compromised host. the virus may be invariable but the host can have diverse susceptibilities. (2) b19 and some other human viruses (though for none is the evidence so clear as for b19) have narrowly targetted effects. the host cell of b19 is a specialised progenitor of mature red cells: impairment of the func ... | 1992 | 1342709 |
| [toxoplasmosis and important virus infections in pregnancy-- diagnosis and management]. | toxoplasmosis and viral infections such as rubella, cytomegaly and parvovirus b19 are much feared risk during pregnancy. the rate of connatal toxoplasmosis and of subclinically infected infants at birth with the risk of late manifestation is still unclear, whereas such data are fairly well-known for rubella-, cmv- and parvovirus-b19-infections. the respective major diagnostic issues in pregnancy, the laboratory diagnosis, and its rational use in combination with clinical information are presente ... | 1992 | 1337060 |
| [24 cases of human parvovirus b19 infection in children]. | from january 1, 1987 through december 31, 1990, twenty-four pediatric patients with human parvovirus b19 (hpv b19) infection were seen. in every case the diagnosis was established by a positive capture immunoassay for igm antibodies against the hpv b19. four patients had hematologic manifestations, including one case of transient bone marrow aplasia revealing hereditary spherocytosis, one case of autoimmune hemolytic anemia with beta-thalassemia, and two cases of peripheral thrombocytopenia. eig ... | 1992 | 1334393 |
| histopathologic variability of finding erythroid inclusions with intrauterine parvovirus b19 infection. | | 1992 | 1448397 |
| chronic parvovirus infection in a presumably immunologically healthy woman. | infection due to parvovirus b19 is common and usually resolves over several weeks. prolonged infection has been reported primarily in immunodeficient hosts. the present report describes a chronic infection in an apparently immunologically healthy woman. the illness was characterized by recurrent episodes of paresthesia without anemia. laboratory studies demonstrated persistence of parvovirus-specific dna for nearly 4 years. | 1992 | 1330011 |
| parvovirus b19 infection: clinical disease update. | | 1992 | 1328450 |
| cytomegalovirus infection, fetal liver disease, and neonatal hemochromatosis. | neonatal hemochromatosis is an uncommon disorder, clinicopathologically defined by severe and generally fatal liver disease of intrauterine onset associated with extrahepatic siderosis that spares reticuloendothelial elements (hemochromatotic siderosis). the agent or agents of liver disease in neonatal hemochromatosis are not known. it also is not known if intrauterine liver disease of defined infective etiology can lead to hemochromatotic siderosis. we present two patients with fetal liver dise ... | 1992 | 1325409 |
| petechial glove and sock syndrome caused by parvovirus b19. | the petechial glove and sock syndrome is a recently described febrile dermatosis characterized by acral pruritus, edema, pain, petechiae, and an enanthem of petechiae and erosions; these features suggest a viral origin. we report a typical case in a 36-year-old woman. igm antibodies to human parvovirus b19 (pvb19) were present, and acute and convalescent igg antibodies demonstrated seroconversion, which suggested recent infection with pvb19. results of tests for other viral and bacterial agents ... | 1992 | 1469139 |
| impact of child day care on infectious diseases in adults. | the authors review diseases that occur in day care centers and their impact on day care providers and parents. acute infections of the gastrointestinal and respiratory tracts are the most commonly transmitted illnesses from children in day care centers to adults. the most important infections acquired by adults are those with the potential for significant clinical morbidity, such as hepatitis a, or those that generate concern, such as cytomegalovirus and parvovirus b19, because of their potentia ... | 1992 | 1315813 |
| spontaneous resolution of nonimmune hydrops fetalis secondary to human parvovirus b19 infection. | many instances of nonimmune hydrops fetalis ascribed to human parvovirus b19 have been reported. the leading proposed pathophysiologic mechanism of hydrops in affected fetuses is viral invasion of red blood cell progenitors, causing a profound reticulocytopenic fetal anemia. although the natural history of fetal parvovirus infection remains to be elucidated fully, there have been recent reports of funipuncuture and intrauterine blood transfusions to diagnose and manage this problem. we report tw ... | 1992 | 1314362 |
| immunohistological detection of human parvovirus b19 in formalin-fixed, paraffin-embedded tissues. | human parvovirus b19 is a cause of aplastic crises in patients with haemolytic anaemias, prolonged bone marrow failure in the immunosuppressed, and fetal death secondary to non-immune hydrops. the immunohistological detection of parvovirus b19 in formalin-fixed, paraffin-embedded tissues has not previously been reported, and definitive diagnosis of infection in such specimens has relied on the use of specialized dna hybridization and amplification techniques. a new monoclonal antibody to b19 cap ... | 1992 | 1313862 |
| laboratory infection with human parvovirus b19. | | 1992 | 1312560 |
| human parvovirus b19 infection in hemophiliacs first infused with two high-purity, virally attenuated factor viii concentrates. | human parvovirus b19 can be transmitted by coagulation factor concentrates and is highly resistant to virucidal methods. to evaluate whether the additional removal of virus by chromatographic methods during the manufacture of high-purity concentrates reduces the risk of b19 transmission, we have prospectively evaluated the rate of anti-b19 seroconversion in two groups of susceptible (anti-b19 negative) hemophiliacs infused with high-purity, heated (pasteurized) or solvent-detergent-treated facto ... | 1992 | 1312304 |
| red cell aplasia caused by human parvovirus b19 in acute leukaemia. | | 1992 | 1308098 |
| [an adult case of transverse myelitis with erythema infectiosum]. | we reported an adult case of transverse myelitis with erythema infectiosum. a 33-year-old female was admitted to kyoto university hospital because of a weakness in the lower extremities and "cloth-wearing" sensation of the trunk and legs. one month before admission, she became febrile and developed a symmetrical erythema on the extremities. at the same time she noticed a slight weakness of the legs and numbness in her fingers and toes, which disappeared next few days. a week later, she again dev ... | 1992 | 1300262 |
| bone marrow necrosis and human parvovirus associated infection preceding an ph1+ acute lymphoblastic leukemia. | a case of bone marrow necrosis associated with a serologically documented recent parvovirus b 19 infection which preceded the development of ph1+ acute lymphoblastic leukemia is reported. no conclusions can be drawn on the basis of a single case but the question of the role of human parvovirus b19 in the pathogenesis of bone marrow necrosis is discussed. it is suggested that the virus may act as a co-factor for the induction of bone marrow necrosis, in some cases. | 1992 | 1290967 |
| koplik spots and a purpuric eruption associated with parvovirus b19 infection. | | 1992 | 1401292 |
| non-isotopic in situ hybridisation and immunophenotyping of infected cells in the investigation of human fetal parvovirus infection. | to compare the use of biotinylated and digoxigenin labelled probes for diagnosis of human fetal parvovirus b19 infection in formalin fixed, paraffin wax embedded tissues; and to assess the cellular distribution of the virus in positive cases. | 1992 | 1401175 |
| viruses and the blood. | haematological syndromes attributed to viruses demonstrate geographical variations in incidence and great dependence on host factors. severe haematological disease is the exception rather than the rule in dengue virus infection, and probably depends at least in part on the host immune response to the virus. the increased incidence of hepatitis-associated aplasia in east asia may reflect distribution of an infectious agent, an environmental toxin, or genetic predisposition, but probably represent ... | 1992 | 1511177 |
| anaemia of plasmodium falciparum malaria. | the pathophysiology of the anaemia of falciparum malaria is both complex and multifactorial, and results in a condition which is a major cause of mortality and morbidity in patients, especially children and pregnant women, living in malarial endemic areas. the importance of anaemia as a cause of death in malaria may well be underestimated because of difficulty in diagnosis, especially where parasitaemia may be low and the clinical picture may be confused with other causes of anaemia. two clinica ... | 1992 | 1511178 |
| parvovirus b19 infection during pregnancy--an ongoing prognostic challenge. | | 1992 | 1396901 |
| laboratory diagnosis of parvovirus b19 infection. | the sensitivity and application of the polymerase chain reaction (pcr) for the diagnosis of parvovirus b19 (b19) infection was investigated by simultaneously assaying a collection of 279 consecutively received samples for presence of anti-b19 igm and igg antibodies by western blot and for b19 dna by pcr and dot-blot hybridization (dot-blot); samples were sera from patients with suspected b19 infection. pcr and dot-blot detected b19 dna in 9% (16/179) and 1% (2/179), respectively of ab-positive s ... | 1992 | 1403335 |
| [kidney involvement in parvovirus b 19 infection in a child]. | | 1992 | 1405390 |
| [parvovirus b19 infection]. | | 1992 | 1408379 |
| complete nucleotide sequence of the cloned infectious genome of junonia coenia densovirus reveals an organization unique among parvoviruses. | we previously constructed a recombinant plasmid, pbrj, encompassing an infectious junonia coenia densovirus (jcdnv) genome (m. jourdan et al. (1990). virology 179, 403-409). we report here the complete viral sequence of pbrj. the genome, 5908 nucleotides (nt) long, consists of an internal unique sequence flanked by long (517 nt) inverted terminal repeats. the first 96 bases of one extremity can fold into a typical y-shaped hairpin structure. the opposite extremity is incomplete, lacking 88 nt. t ... | 1992 | 1413502 |
| immunophenotyping of fetal haemopoietic cells permissive for human parvovirus b19 replication in vitro. | human parvovirus b19 is known to inhibit erythroid colony formation in vitro, but the precise stage of differentiation at which erythroid precursors become capable of supporting viral replication has not been accurately determined. in order to address this issue, haemopoietic cells derived from first trimester fetal liver were cultured in medium containing b19 antigen-positive serum. infected cells were phenotyped by combining immunohistology for cell-type specific antigens with non-isotopic in ... | 1992 | 1419811 |
| hypoplastic anemia in a hemophiliac first infused with a solvent/detergent treated factor viii concentrate: the role of human b19 parvovirus. | | 1992 | 1550108 |
| two anti-parvovirus b 19 igm capture assays incorporating a mouse monoclonal antibody specific for b 19 viral capsid proteins vp 1 and vp 2. | during an outbreak of parvovirus b 19 in 1989 in northern ireland, 7580 blood donors were screened for b 19 antigen. two units screened positive, one of which was obtained for use as viral antigen. a monoclonal antibody (r92f6) made against this antigen was specific for b 19 capsid proteins vp 1 and vp 2. the monoclonal antibody was used in the development of 2 anti-b 19 igm capture enzyme assays. these used a conventional substrate (o-phenylene diamine) and a chemiluminescent signal reagent. th ... | 1992 | 1550492 |
| recombinant parvovirus b19 capsids as a new substrate for detection of b19-specific igg and igm antibodies by an enzyme-linked immunosorbent assay. | a new enzyme-linked immunosorbent assay for the detection of b19-specific igg and igm antibodies was established using b19 capsids synthesized in a baculovirus expression system. these b19 capsids, consisting of either coat protein vp2 alone or of both vp1 and vp2, have been shown to be similar to native virus in size and appearance. the results obtained for the detection of b19-specific antibodies showed good correlations with a radioimmunoassay which uses native b19 virus and an immunofluoresc ... | 1992 | 1430069 |
| localization of an immunodominant domain on baculovirus-produced parvovirus b19 capsids: correlation to a major surface region on the native virus particle. | an immunodominant region on baculovirus-produced parvovirus b19 vp2 capsids was localized between amino acids 259 and 426 by mapping the binding sites of a panel of monoclonal antibodies which recognize determinants on the particles. the binding sites of three monoclonal antibodies were fine-mapped within this antigenic domain. six vp2-specific monoclonal antibodies recognized determinants common to both the empty capsids and native parvovirus. the defined antigenic region is most probably expos ... | 1992 | 1433504 |
| lupus-like presentation of human parvovirus b19 infection. | the diagnosis of systemic lupus erythematosus (sle) was a leading initial consideration in 2 patients with rash, arthritis and hypocomplementemia. one patient also had leukopenia and thrombocytopenia. spontaneous regression occurred. in both patients antinuclear antibodies were negative. serologic studies indicated recent human parvovirus b19 infection. we propose adding human parvovirus b19 infection to the list of conditions that may masquerade as sle. | 1992 | 1556683 |
| a significant age shift of the human parvovirus b19 antibody prevalence among young adults in japan observed in a decade. | a seroepidemiological study on human parvovirus (b19) in japan was undertaken with serum samples randomly collected from healthy japanese populations (416 in 1973, 675 in 1984 and 508 in 1987/88). all samples were tested for anti-b19 igg antibody by the indirect antigen-capture elisa. the antibody prevalence for ages 0-9 years old in 1984 was significantly higher (16%) than that in 1973 (2%), whereas those for ages 20-29 years and 30-39 years were significantly lower in 1984 (20% and 56%) than i ... | 1992 | 1434067 |
| parvovirus infection of the human fetus and newborn. | human parvovirus b19 is a recently recognized cause of hydrops fetalis. it is a small, single-stranded dna virus, which preferentially infects late erythroid precursors and produces red blood cell (rbc) aplasia, fetal anemia, and cardiac failure. infection is accompanied by characteristic intranuclear inclusions in fixed and circulating rbc precursors. these inclusions have been shown to contain virus particles by electron microscopy and in situ hybridization. infection of the fetus, mother, and ... | 1992 | 1561488 |
| aplastic crisis in haemolytic anaemia due to infection parvovirus b19. | | 1992 | 1437848 |
| persistence of b19 parvovirus in synovial membranes of patients with rheumatoid arthritis. | recent clinical observations support the hypothesis that persistent parvovirus b19 is a triggering factor of rheumatoid arthritis (ra) in certain genetically predisposed individuals. if this hypothesis is correct, a number of ra patients may exhibit parvovirus b19 dna in their synovial membranes. we tested the synovial tissue and peripheral blood leukocytes of 20 patients with ra, 24 patients with other arthritides or osteoarthritis (non-ra), and 34 healthy blood donors for the presence of parvo ... | 1992 | 1439481 |
| prevalence of antibodies to parvovirus b19 in selected groups of patients and healthy individuals. | the prevalence of antibodies to parvovirus b19 in sera (n = 745) of various groups of patients and healthy individuals was determined by the enzyme immunoassay, using viral particles as antigen. among healthy individuals, anti-b19 igg prevalence was highest in nurses (65.4% (17/26)); in medical students it was 34.1% (47/138) and in pregnant females, 24.4% (48/197). 37.0% (44/119) of hiv-negative haemophiliac patients and 91.7% (33/36) of haemophilic patients with hiv infection were anti-b19 igg- ... | 1992 | 1576413 |
| parvovirus b19 infection during acute plasmodium falciparum malaria. | | 1992 | 1464364 |
| [epidemiologic characteristics of human parvovirus b19. report of an epidemic of erythema infectiosum in the area of belgrade]. | during the period 29.10.1987-10.5.1988 an outbreak of an illness with rash resembling erythema infectiosum occurred among school children in one part of belgrade-mladenovac. this is the first outbreak of erythema infectiosum reported in yugoslavia and confirmed in university college of middlesex school of medicine, london, in november 1989. of 720 school children exposed to infection of human parvovirus b 19 two hundred and eighty four (39.4%) have had clinical symptoms and 166 (58.4%) of them w ... | 1992 | 1465672 |
| different syndromes associated with b19 parvovirus viraemia in paediatric patients: report of four cases. | the broad spectrum of clinical manifestations associated with b19 parvovirus often allows the infection to go unrecognized. we tested for the presence of b19 parvovirus and the specific serological response in serum from hospitalized patients submitted for viral investigations without any specific indications for b19 parvovirus. we diagnosed human parvovirus b19 infection in four paediatric patients showing different clinical manifestations. the patients, aged between 5 and 8 years, were admitte ... | 1992 | 1468455 |
| in vitro culture for the detection of infectious human parvovirus b19 and b19-specific antibodies using foetal haematopoietic precursor cells. | the inability to culture human parvovirus b19 in standard cell lines has rendered investigation of clinical samples for the presence of infectious virus problematic. using haematopoietic precursors derived from first trimester foetal liver as targets for infection, and non-isotopic in situ hybridization to detect intracellular viral dna, we have assessed infectivity in stored serum samples taken from nine volunteers at different stages following intranasal inoculation with parvovirus b19. infect ... | 1992 | 1469369 |
| the fifth cause of splenomegaly?--parvovirus b19. | | 1992 | 1478012 |
| [fetal erythropoiesis and parvovirus b19]. | target cells for the human parvovirus b19 include erythroid progenitors located in the bone marrow in adults and in the liver in fetuses of 12 to 30 weeks gestational age. the main manifestations of fetal parvovirus b19 infection seem to be related to lysis of the erythroid progenitors which causes non immune hydrops fetalis with severe anemia, congestive heart failure, generalized edema and death. the exact incidence of human parvovirus b19 infection during pregnancy remains unclear. | 1992 | 1480406 |
| human parvovirus b19 and rheumatoid arthritis. | human parvovirus b19 infections have been linked with the development of a short-lived symmetrical polyarthritis and, rarely, a more persistent arthritis. we prospectively looked for serological evidence of recent b19 infection in 25 early synovitis patients presenting within 12 weeks of symptom onset and compared them with 21 controls seen over the same time period. none of the control patients had evidence of recent b19 infection while 3 of the early synovitis patients had raised igm anti-b19 ... | 1992 | 1486749 |
| long-term outcome in fetal hydrops from parvovirus b19 infection. | parvovirus b19 infection in the fetus is associated with anemia and hydrops and can result in fetal death. fetal transfusion has been used in an attempt to improve outcome; however, it is associated with its own perinatal morbidity. we report two cases of fetal parvovirus b19 infection that were confirmed by polymerase chain reaction for parvovirus b19 deoxyribonucleic acid in umbilical cord blood. ultrasonographic signs of compromise were observed at 30 and 24 weeks of gestation. both fetuses w ... | 1992 | 1497035 |
| prenatal diagnosis of human parvovirus b19 in nonimmune hydrops fetalis by polymerase chain reaction. | nonimmune hydrops fetalis is a potentially lethal condition reflecting the clinical manifestation of several pathologic processes. recently maternal infection by human parvovirus b19 has been reported to result in nonimmune fetal hydrops. we sought to develop a rapid and sensitive test to detect the presence of this agent in utero. | 1992 | 1497052 |
| [autoimmune neutropenia and parvovirus b19 infection]. | | 1992 | 1497433 |
| [acute vasculitis and arthritis caused by parvovirus b 19 infection]. | a 35-year old woman was admitted with fever, polyarthritis and severe vasculitis. the symptoms resolved spontaneously within a week. two weeks later, her seven year old son developed erythema on arms and legs and the typical "slapped cheek syndrome" characteristic of erythema infectiosum. both cases were due to acute infection with parvovirus b 19 as demonstrated by the presence of igm-antibodies to parvovirus b 19. the cases are described in order to draw attention to the possibility of parvovi ... | 1992 | 1509571 |
| heat stability of parvovirus b19: kinetics of inactivation. | heat inactivation of parvovirus b19 (b19) was studied in a culture of hematopoietic progenitor cells generated in vitro from peripheral human blood. after inoculating cell cultures with identical volumes of plasma (mii) containing b19 (b19-mii) heat-treated (60 degrees c) for various periods of time, a time-dependent inactivation of the input virus was determined by a decrease of viral dna replication. no b19 dna was detected after infection with b19-mii heat-treated for 20 min or more by southe ... | 1992 | 1520981 |
| clinical and histopathological features of parvovirus b19 infection in the human fetus. | to provide a comprehensive description of the clinical and histopathological features associated with parvovirus b19 infection of the human fetus. | 1992 | 1525097 |
| transient erythroblastopenia of childhood with megakaryocytopenia associated with human parvovirus b19 infection. | | 1992 | 1536802 |
| prokaryotic expression of a vp1 polypeptide antigen for diagnosis by a human parvovirus b19 antibody enzyme immunoassay. | to produce parvovirus b19 antigen for diagnostic purposes, partially overlapping segments covering the genes encoding the viral structural proteins vp1 and vp2 were cloned into expression vectors. the constructs were induced in escherichia coli, resulting in the expression of beta-galactosidase fusion proteins. in immunoblotting experiments with sera from patients with erythema infectiosum, immunoglobulin g (igg) and igm antibodies bound to a single polypeptide of 235 amino acids at the n termin ... | 1992 | 1537897 |
| red cell aplasia responsive to immunoglobulin therapy as initial manifestation of human immunodeficiency virus infection. | a patient who presented with pure red cell aplasia as the initial manifestation of human immunodeficiency infection is described. the patient had no signs of other autoimmune processes or immunologic or histologic evidence of parvovirus b19 infection, although we detected parvovirus b19 dna in his serum. this patient had a complete, although temporary, response to a single course of immunoglobulin therapy and is currently responding to a second treatment. | 1992 | 1546732 |
| parvovirus b19 infection of the fetus. histology and in situ hybridization. | fetal tissues from 16 spontaneous abortions, two terminations, and one perinatal death, 18 of which were associated with maternal human parvovirus b19 infection, were examined for b19 infection by histology and in situ hybridization using a digoxigenin-labeled b19-dna probe. in 15 spontaneous abortions and one termination, erythroblasts with intranuclear inclusions (lantern cells) reacted with b19-dna by in situ hybridization. no internal or external fetal malformations were observed. because 13 ... | 1991 | 1648876 |
| parvovirus b19, hydrops fetalis, and fetal wastage. an etiologic sequence. | | 1991 | 1648877 |
| fifth disease and other parvovirus b19 infections. | parvovirus b19 is the etiologic agent in three diseases with clinically different presentations and implications. the first and most common disease is erythema infectiosum or fifth disease. symptoms may vary somewhat between affected children and adults. a second type of presentation occurs in infected people who require a greater than normal replacement of red blood cells, and a third infection may affect a developing fetus. because there is no effective treatment or vaccine, physicians and nur ... | 1991 | 1649150 |
| laboratory infection with human parvovirus b19. | | 1991 | 1649224 |
| parvovirus b19 for the hematologist. | | 1992 | 1550103 |
| the clinical spectrum of human parvovirus b19 infections. | | 1991 | 1651089 |
| human parvovirus b19 and its associated diseases. | from the point of view of obstetrics and pediatrics, the importance of b19 infection lies in the potential for intraueterine infection following maternal b19 infection. ongoing prospective studies of b19 infection in pregnant women indicate a risk of adverse pregnancy outcomes considerably lower than previous estimates from retrospective studies and case reports. the occurrence, however, of severe nonimmune hydrops or stillbirths or both in even a small percentage of progeny of b19-infected preg ... | 1991 | 1652405 |
| evaluation of a synthetic-peptide enzyme-linked immunosorbent assay for immunoglobulin m to human parvovirus b19. | a synthetic peptide corresponding to a part of the virus protein 1-virus protein 2 overlapping region of human parvovirus b19 was used in an indirect enzyme-linked immunosorbent assay. antibodies of the immunoglobulin (ig) m class were measured in serum samples from patients with erythema infectiosum and controls. in comparison with an igm assay using native b19 viral antigen, the peptide antigen assay was 92% sensitive and 87% specific. b19 igm reactivities were seen in a limited number of chil ... | 1991 | 1653264 |
| fetal survival after human parvovirus b19 infection: spectrum of intrauterine response in a twin gestation. | we report the first known symptomatic survivors of congenital parvovirus infection. one fetus was hydropic and the other was growth retarded. there was no evidence of anemia. the antenatal diagnosis of congenital infection requires a multifaceted approach, which includes serology, cultures, and electron microscopy. | 1992 | 1550638 |
| recurrent granulocytic aplasia as clinical presentation of a persistent parvovirus b19 infection. | we report a case of persistent infection with human parvovirus b19 (pvb19), manifesting clinically as recurrent agranulocytosis and in bone marrow biopsy as recurrent pure granulocytic aplasia. persistence of parvovirus infection was documented by the presence of pvb19 dna and anti-pvb19-igm antibodies in the serum for a period of 19 months. granulocytic aplasia occurred only when anti-pvb19-igg antibodies were not detectable in the serum and granulopoiesis showed immediate recovery with high do ... | 1992 | 1550770 |
| parvovirus b19 and human disease. | | 1992 | 1558006 |
| viruses and bone marrow failure. | many agents are associated with bone marrow failure, including toxins, inherited metabolic defects, ionizing radiation, and viral infection. in most cases, the etiologic agent is unknown. many of these unclassified cases have symptomatic, immunologic, or epidemiologic similarities to viral infections. viruses from different taxonomic families have been implicated in bone marrow failure syndromes, and they appear to cause hematosuppression by a variety of mechanisms. some of the viruses involved ... | 1991 | 1655129 |
| prenatal diagnosis of intrauterine infection with parvovirus b19 by the polymerase chain reaction technique. | human parvovirus b19 is a recently recognized cause of fetal hydrops and death. efforts to characterize the natural history of fetal infection with this virus have been hampered by the lack of sensitive and specific tests for diagnosis in utero. using the highly sensitive polymerase chain reaction (pcr) assay, we determined the fetal infection status in 56 pregnancies by testing amniotic fluid, fetal serum, and maternal serum for b19 dna and antibodies. factors associated with a high risk of b19 ... | 1992 | 1571420 |
| [3 cases of kituchi's lymphadenitis in systemic lupus erythematosus. role of the parvovirus b19]. | florid necrotizing lymphadenitis, characterized by segmental infarction and lymphoid hyperplasia, is an uncommon feature of systemic lupus erythematosus (sle). kikuchi's disease is a well-defined clinicopathological entity, with a strong preference for the cervical lymph nodes of young women. the etiology of histiocytic necrotizing lymphadenitis (hnl) remains unknown, although viral agents have been proposed. hnl may reflect a self-limited sle-like autoimmune disease but full-blown sle associate ... | 1991 | 1656832 |
| fetal medicine. | fetal therapy continues to be an exciting yet controversial field. in utero treatment of a variety of fetal conditions is discussed: parvovirus b19 infection, fetal thyroid dysfunction, fetal ovarian cysts, twin-twin transfusion syndrome, and fetal hemolytic disease. fetal surgery continues to be controversial. despite considerable publicity, fetal surgery appears to be rarely, if ever, truly indicated. a new area of fetal therapy, ie, antenatal transplantation of fetal liver stem cells, is disc ... | 1992 | 1571488 |
| replication and cytopathology of human parvovirus b19 in human umbilical cord blood erythroid progenitor cells. | human parvovirus b19 productively infected erythroid progenitor (ep) cells from umbilical cord blood, in vitro as shown by an increase of viral dna in supernatant fluid assayed by dot blot hybridization and liquid scintillation counting. progeny virus was released into the supernatant fluid of cd34+ ep cells which had been purified by immunomagnetic separation. this supernatant fluid was infectious for bone marrow cells. erythroid bursts infected with virus showed characteristic cytopathic effec ... | 1992 | 1583466 |
| non-permissiveness of synovial membrane cells to human parvovirus b19 in vitro. | the ability of cultured human synovial cells derived from synovial membrane and cartilage to support the replication of human parvovirus b19 was assessed. no viral dna synthesis nor viral antigens were detected suggesting that b19 virus is not capable of replicating in synovial cells. the significance of this finding in relationship to the pathogenesis of parvovirus arthritis is discussed. | 1992 | 1607873 |
| pure red-cell aplasia due to parvovirus b19 infection in a patient with aids-related complex. | | 1992 | 1610541 |
| possible induction of systemic lupus erythematosus by human parvovirus. | a 59 year old woman presented with an influenza-like illness preceding signs and symptoms strongly suggestive of systemic lupus erythematosus (sle), which progressed over several months. owing to these influenza-like symptoms, a viral cause of her illness was sought. human parvovirus b19 serology was positive and antibodies to dna were detected by two different methods. this patient is believed to be the first report of human parvovirus b19 infection coinciding with the onset of sle. the evidenc ... | 1992 | 1616368 |
| nosocomial human parvovirus b19 infection: lack of transmission from a chronically infected patient to hospital staff. | to assess the potential for nosocomial spread of parvovirus b19 from a chronically infected patient. | 1992 | 1619271 |
| [sero-diagnosis for human parvovirus b19 infection by igm and igg antibody capture method of enzyme-linked immunosorbent assay--study on an epidemic case of erythema infectiosum]. | the igm and igg antibody capture methods of the enzyme-linked immunosorbent assay (elisa) for human parvovirus b19 were performed using horseradish peroxidase (hrpo)-labeled anti b19 monoclonal antibody. serially obtained serum samples from one erythema infectiosum (e.i.) patient were examined at once by this methods. the dod values of the igm and igg antibodies decreased on the typical curves according to the course of recovery. in the epidemic case of e.i. among students of one nurse school, 1 ... | 1992 | 1624835 |
| infectious etiology of rheumatoid arthritis. | the infectious etiology of rheumatoid arthritis has been a long-standing hypothesis and in recent years is being examined with greater sophistication and scientific rigor. synovitis may result indirectly from infection by the deposition of circulating immune complexes, by molecular mimicry, by in situ antigen deposition, or by arthritogenic toxins. of candidate pathogens, recent interest has focused on mycobacterial hsp, ebv, and parvovirus b19. there is circumstantial evidence to support a link ... | 1991 | 1662820 |
| human parvovirus b-19: not just a pediatric problem. | parvoviruses have long been associated with disabling and even fatal illnesses in animals. the discovery of the human parvovirus b-19 in 1975 (1) and subsequent studies of its effects in humans identified this virus as the causative agent of erythema infectiosum ("fifth disease") in children. (2). erythema infectiosum (ei) is a common, self-limited infectious disorder in children, easily recognized by the classic "slapped cheek" facial erythema and fine reticular rash. only in the 1980s have fur ... | 1992 | 1629590 |
| parvovirus infection in children with aids: high prevalence of b19-specific immunoglobulin m and g antibodies. | investigation of the prevalence and pathogenic role of parvovirus b19 infection in italian and rumanian children with aids, compared with age-matched hiv-negative children (controls) with various recurrent infections of unknown aetiology. | 1992 | 1503687 |
| polymerase chain reaction with double primer pairs for detection of human parvovirus b19 induced aplastic crises in family outbreaks. | parvovirus b19 dna can be detected by polymerase chain reaction with double primer pairs (nested pcr). recent infection was documented by a retrospective serological study using parvoscan-b19 enzyme linked immunosorbent assay (eia) for detection of b19 human parvovirus igm and igg antibodies in serum or plasma specimens. in 3 families b19 outbreaks caused aplastic crises necessitating blood transfusion in 5 children and 1 adult with hereditary sphaerocytosis. four members from 2 of the families ... | 1992 | 1509232 |
| parvovirus b19 replication in human umbilical cord blood cells. | the human parvovirus b19 is now known to be one of the causative agents of nonimmune hydrops fetalis and spontaneous abortions in pregnant women. the presence of the viral proteins and antibodies in fetuses of b19-infected women suggests that the virus can cross the placental barrier. in order to gain an insight into the mechanism of intrauterine fetal infection and the virus-induced hydrops fetalis, we examined whether human umbilical cord blood cells were permissive for b19 replication. cord b ... | 1992 | 1641977 |
| haemagglutination by parvovirus b19. | human parvovirus b19 is a member of the autonomous parvoviridae but in contrast to other members of the genus has not been shown to agglutinate red blood cells. we now report that the virus agglutinates red cells of primate origin, though with plasma-derived virus this activity is masked by the presence of an igm-like inhibitor. this observation is consistent with the presence on the erythroid precursor target cell of a specific receptor for parvovirus b19. | 1992 | 1645153 |
| manifestations and treatment of human parvovirus b19 infection in immunocompromised patients. | we diagnosed infections from human parvovirus b19 in three patients by using dot-blot hybridization and a polymerase chain reaction to detect b19 dna and using an enzyme immunoassay to detect igg and igm to b19. for 5 months a 5-year-old boy with acute lymphoblastic leukemia in remission had anemia without reticulocytes or bone marrow erythrocyte precursors. his serum lacked igg and igm to b19 but contained b19 dna. he received gamma globulin intravenously (0.4 gm/kg/day for 5 days); his viremia ... | 1990 | 1689774 |
| new oligopeptide immunoglobulin g test for human parvovirus b19 antibodies. | a new, highly sensitive and specific enzyme immunoassay using oligopeptides as antigen (enzyme-linked immunosorbent assay [elisa] b19-op) for detecting parvovirus b19-specific immunoglobulin g (igg) was established. as antigens, b19-specific oligopeptides of 24 and 30 kda derived from a 196-kda fusion protein of beta-galactosidase and viral capsid protein (vpi) of b19 after cnbr cleavage and separation by high-pressure liquid chromatography were used. of 139 serum specimens tested in parallel fo ... | 1991 | 1645365 |
| chronic human parvovirus b19-induced erythroid hypoplasia as the initial manifestation of human immunodeficiency virus infection. | | 1991 | 1645771 |
| detection of b19 parvovirus in human fetal tissues by in situ hybridisation. | evidence of b19 parvovirus infection was sought by in situ hybridisation with biotinylated probes in 65 tissue samples from 32 pregnancies (fetuses, products of conception and/or placentas). twenty-seven samples were reactive and the results were confirmed by other methods for b19 virus detection in 22 cases. the other methods used were in situ hybridisation with 3h and 35s labelled probes; dot-blot hybridisation with biotin and 32p labelled probes; polymerase chain reaction assay; negative stai ... | 1991 | 1646854 |
| chemiluminescent assay for the detection of viral and plasmid dna using digoxigenin-labeled probes. | a dot-blot hybridization immunoenzymatic assay with a chemiluminescent endpoint was developed for the rapid and sensitive detection of viral and plasmid dnas. digoxigenin-labeled probes were used to detect cytomegalovirus, parvovirus b19, and plasmid pbr328 dnas. hybridized probes were immunoenzymatically visualized by anti-digoxigenin fab fragments labeled with alkaline phosphatase, and adamantyl 1,2-dioxetane phenyl phosphate was used as chemiluminescent substrate. results were recorded by ins ... | 1991 | 1650541 |
| [molecular biological demonstration of parvovirus infection in fetal tissue]. | we report on the morphological findings in 16 fetuses with serologically confirmed maternal parvovirus b19 infection. typical routine morphological findings of the hydropic fetuses were the presence of abnormal erythroblasts with typical nuclear inclusions. these infected cells positively stained immunohistochemically with antibodies against a recombinant virus protein, as well as they ultrastructurally contained virus particles. using in-situ hybridization techniques, we were able to demonstrat ... | 1990 | 1708624 |