Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
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| Detection of plum pox potyviral protein-protein interactions in planta using an optimized mRFP-based bimolecular fluorescence complementation system. | In previous studies, protein interaction maps of different potyviruses have been generated using yeast two-hybrid (YTH) systems, and these maps have demonstrated a high diversity of interactions of potyviral proteins. Using an optimized bimolecular fluorescence complementation (BiFC) system, a complete interaction matrix for proteins of a potyvirus was developed for the first time under in planta conditions with ten proteins from plum pox virus (PPV). In total, 52 of 100 possible interactions we ... | 2011 | 21880839 |
| Seroprevalence of porcine reproductive and respiratory syndrome, Aujeszky's disease, and porcine parvovirus in replacement gilts in Thailand. | The present study investigated the seroprevalence of porcine reproductive and respiratory syndrome virus, Aujeszky's disease virus (ADV), and porcine parvovirus (PPV) in replacement gilts from selected five swine herds in Thailand. The study consisted of three parts. First, a retrospective data analysis on the seroprevalence of porcine reproductive and respiratory syndrome virus (PRRSV) and ADV glycoprotein I (gI) in gilts, sows, boars, nursery, and fattening pigs in five herds (n?=?7,030). Seco ... | 2011 | 22068636 |
| isolated anti-hbc-igm antibody among blood donors in the semi-arid region of nigeria. | laboratory screening for the diagnosis of hepatitis b virus (hbv) infection in blood donors currently consists of testing for hepatitis b surface (hbsag) antigen alone. the prevalence of isolated anti-hbc-igm is not yet known in the semi arid region of nigeria. the major objective of this study was to determine the sero-prevalence of antibody to hepatitis b core antigen (anti-hbc-igm) and other infectious agent markers; hbsag, hcv, hiv and syphilis among blood donors in the north eastern region ... | 2011 | 22129677 |
| detection of porcine circovirus type 1 in commercial porcine vaccines by loop-mediated isothermal amplification. | a loop-mediated isothermal amplification (lamp) method with a real-time monitoring system was developed for the detection of porcine circovirus type 1 (pcv1) in commercial swine vaccines. this method was highly specific for pcv1. no cross-reaction to porcine circovirus type 2, porcine parvovirus, pseudorabies virus, classical swine fever virus, and porcine reproductive and respiratory syndrome virus was observed. the analytical sensitivity of the lamp for pcv1 dna was 10 copies/μl in the case of ... | 2011 | 21948286 |
| using polymer conformation to control architecture in semiconducting polymer/viral capsid assemblies. | cowpea chlorotic mottle virus is a single-stranded rna plant virus with a diameter of 28 nm. the proteins comprising the capsid of this virus can be purified and reassembled either by themselves to form hollow structures or with polyanions such as double-stranded dna or single-stranded rna. depending on ph and ionic strength, a diverse range of structures and shapes can form. the work presented here focuses on using these proteins to encapsulate a fluorescent polyanionic semiconducting polymer, ... | 2011 | 21942298 |
| [treatment of chronic hepatitis c virus infection. a study of best predictors for response.] | objective: the aim of this study was evaluate the rate of sustained viral response (svr) and the influence of different factors on the svr in patients with chronic hepatitis c virus (hcv) infection treated with pegylated interferon alfa 2a and ribavirin. methods: we retrospectively analysed 272 naïve patients with chronic hepatitis c who had been treated for 24 weeks or 48 weeks and had been followed for an additional 6 months thereafter. results: out of 272 patients, 243 completed the entire tr ... | 2011 | 22173189 |
| comparison of conventional cervical cytology versus visual inspection with acetic acid among human immunodeficiency virus-infected women in western kenya. | objective: this study aimed to determine the accuracy of visual inspection with acetic acid (via) versus conventional pap smear as a screening tool for cervical intraepithelial neoplasia/cancer among human immunodeficiency virus (hiv)-infected women. materials and methods: a total of 150 hiv-infected women attending the moi teaching and referral hospital hiv clinic in eldoret underwent conventional pap smear, via, colposcopy, and biopsy. both via and pap smears were done by nurses, whereas colpo ... | 2011 | 22126834 |
| development of a loop-mediated isothermal amplification method for rapid detection of porcine boca-like virus. | the porcine boca-like virus (pbo-likev) was recently discovered in swedish pigs with post-weaning multisystemic wasting syndrome (pmws). in this study, a loop-mediated isothermal amplification (lamp) assay was developed for rapid, specific and sensitive detection of pbo-likev. a set of four primers specific for six regions of pbo-likev vp1/2 genes was designed with the online software. the reaction temperature and time were optimized to 65°c and 60min, respectively. lamp products were detected b ... | 2011 | 22172971 |
| evaluation of the human host range of bovine and porcine viruses that may contaminate bovine serum and porcine trypsin used in the manufacture of biological products. | current u.s. requirements for testing cell substrates used in production of human biological products for contamination with bovine and porcine viruses are u.s. department of agriculture (usda) 9cfr tests for bovine serum or porcine trypsin. 9cfr requires testing of bovine serum for seven specific viruses in six families (immunofluorescence) and at least 2 additional families non-specifically (cytopathicity and hemadsorption). 9cfr testing of porcine trypsin is for porcine parvovirus. recent con ... | 2011 | 22000165 |
| Relative size selection of a conjugated polyelectrolyte in virus-like protein structures. | A conjugated polyelectrolyte poly[(2-methoxy-5-propyloxy sulfonate)-phenyl-ene vinylene] (MPS-PPV) drives the assembly of virus capsid proteins to form single virus-like particles (VLPs) and aggregates with more than two VLPs, with a relative selection of high molecular weight polymer in the latter. | 2011 | 22121498 |
| molecular detection of torque teno sus virus in lymphoid tissues in concomitant infections with other porcine viral pathogens. | in this study, 40 pigs with respiratory and wasting disorders from cuban swine herds were screened by pcr for the presence of ttsuv1, ttsuv2, pcv-2, ppv and csfv in spleen samples. the variability of the porcine ttsuv sequences obtained was investigated by phylogenetic analysis. this study showed for the first time that ttsuv1 and ttsuv2 were present in cuban swine herds. the investigation revealed the following infection rates: ttsuv1 40%, ttsuv2 37.5%, pcv-2 70%, ppv 37.5% and csfv in 52.5%. t ... | 2011 | 21482428 |
| vitamin e and selenium levels are within normal range in pigs diagnosed with mulberry heart disease and evidence for viral involvement in the syndrome is lacking. | mulberry heart disease (mhd) in pigs is characterized by lesions of acute haemorrhagic myocarditis and myocardial necrosis. the objectives of this study were to determine the levels of vitamin e and selenium and 13 other trace minerals in heart and liver tissues and to determine the prevalence of certain viral infections in heart tissues from mhd-affected and mhd-unaffected pigs and the vitamin e and selenium concentration in feed samples from selected farms with mhd. based on the pathological e ... | 2011 | 21518323 |
| age-stratified performance of the cervista hpv 16/18 genotyping test in women with asc-us cytology. | background: the objective of this study was to evaluate the clinical performance of the cervista hpv 16/18 genotyping test for detection of human papilloma virus (hpv) 16 and 18 in cervical cytology specimens in women stratified by age. methods: in a multicenter, prospective clinical study, thinprep specimens were tested for the presence of hpv 16 and 18 using the hpv 16/18 genotyping test. genotyping results from women with atypical squamous cells of undetermined significance or greater cytolog ... | 2011 | 21527581 |
| intrahepatic response markers in chronic hepatitis b patients treated with peginterferon alfa-2a and adefovir. | background: we investigated whether intrahepatic markers could predict response in chronic hepatitis b patients treated with peg-ifn and adefovir for 48 weeks. methods: intrahepatic cccdna, total intrahepatic hbv dna and the proportion of hbsag and hbcag positive hepatocytes in 16 hbeag positive and 24 hbeag negative patients were measured at baseline and at the end of treatment. results: baseline intrahepatic markers were not associated with sustained virological response (svr) defined as hbv d ... | 2011 | 21557773 |
| inactivation of enveloped and non-enveloped viruses on seeded human tissues by gamma irradiation. | human tissue allografts are widely used in a variety of clinical applications with over 1.5 million implants annually in the us alone. since the 1990s, most clinically available allografts have been disinfected to minimize risk of disease transmission. additional safety assurance can be provided by terminal sterilization using low dose gamma irradiation. the impact of such irradiation processing at low temperatures on viruses was the subject of this study. in particular, both human tendon and co ... | 2011 | 21809182 |
| high rate of viral evolution in the capsid protein of porcine parvovirus. | in the last years, it has been shown that some parvoviruses exhibit high substitution rates, close to those of rna viruses. in order to monitor and determine new mutations of the porcine parvovirus (ppv), recent ppv field isolates from austria, brazil, germany and switzerland were sequenced and analyzed. these samples, together with sequences retrieved from genbank, were included in three datasets (consisting of vp2 complete gene, vp2 partial gene and ns1 complete gene). for each dataset, nucleo ... | 2011 | 21795474 |
| diagnostic significance of plasma osteopontin in hepatitis c virus-related hepatocellular carcinoma. | outcome of hepatocellular carcinoma (hcc) depends mainly on its early diagnosis. the performance of traditional biomarkers is not satisfactory. osteopontin (opn) is of potential importance. this study aim to assess the diagnostic value of plasma opn compared with alpha-fetoprotein (afp) for the diagnosis of hcv- related hcc. | 2011 | 21677331 |
| Postweaning multisystemic wasting syndrome produced in gnotobiotic pigs following exposure to various amounts of porcine circovirus type 2a or type 2b. | In late 2005, a postweaning, high mortality syndrome spread rapidly through finishing barns in swine dense areas of the United States. Diagnostic investigations consistently detected porcine circovirus type 2 (PCV2) from diseased tissues. Subsequent genetic analysis revealed that the infectious agent was a PCV2 type termed "PCV2b". Prior to late 2004, only the PCV2a type, but not PCV2b, had been reported in North America. In this communication, we produce severe postweaning multisystemic wasting ... | 2011 | 21684696 |
| analysis of the epitope structure of plum pox virus coat protein. | typing of the particular plum pox virus (ppv) strain responsible in an outbreak has important practical implications and is frequently performed using strain-specific monoclonal antibodies (mabs). analysis in western blots of the reactivity of 24 mabs to a 112-amino-acid n-terminal fragment of the ppv coat protein (cp) expressed in escherichia coli showed that 21 of the 24 mabs recognized linear or denaturation-insensitive epitopes. a series of eight c-truncated cp fragments allowed the mapping ... | 2011 | 21171886 |
| performance of parallel screening of brazilian blood donors with two human immunodeficiency virus immunoassays: implications for sequential immunoassay testing algorithms in other countries. | in brazil it is mandatory to screen donors for human immunodeficiency virus (hiv) antibodies using two immunoassays (ias) in parallel. confirmatory testing is performed only on reactive donors who return for counseling. the goal of this analysis was to determine if concordant ia reactivity accurately predicts infection and can be used for hiv incidence and/or prevalence analyses. | 2011 | 20633245 |
| genetic elements in the vp region of porcine parvovirus are critical to replication efficiency in cell culture. | factors controlling porcine parvovirus (ppv) replication efficiency are poorly characterized. two prototype strains of ppv, nadl-2 and kresse, differ greatly in pathogenic capacity both in vivo and in vitro, yet their genomic sequence is nearly identical (13 single-nucleotide substitutions and a 127-nucleotide noncoding repeated sequence). we have created a series of chimeras of these strains to identify the genetic elements involved in replication efficiency in the host porcine cell line. while ... | 2011 | 21209104 |
| [seroprevalence of rubella virus, varicella zoster virus, cytomegalovirus and parvovirus b19 among pregnant women in the sousse region, tunisia]. | the aim of the study is to evaluate seroprevalence of rubella virus (rv), cytomegalovirus (cmv), varicella zoster virus (vzv), and parvovirus b19 (pb19) in 404 tunisian pregnant women, and to determine reliability of maternal past history of eruption. sociodemographic characteristics, risk factors, and past history of eruption were collected through a questionnaire. serologic tests were performed using enzyme immunoassays. risk factors were analyzed using univariate and multivariate logistic reg ... | 2011 | 21243459 |
| two new subfamilies of dna mismatch repair proteins (muts) specifically abundant in the marine environment. | muts proteins are ubiquitous in cellular organisms and have important roles in dna mismatch repair or recombination. in the virus world, the amoeba-infecting mimivirus, as well as the recently sequenced cafeteria roenbergensis virus are known to encode a muts related to the homologs found in octocorals and ɛ-proteobacteria. to explore the presence of muts proteins in other viral genomes, we performed a genomic survey of four giant viruses ('giruses') (pyramimonas orientalis virus (pov), phaeocys ... | 2011 | 21248859 |
| effects of varying virus-spiking conditions on a virus-removal filter planovaôäó 20n in a virus validation study of antibody solutions. | we aimed to investigate the effect of virus-spiking conditions on the filter performance (flux, flux decay, and parvovirus reduction) of the small virus filter planovaôäó 20n. we used three kinds of porcine parvovirus (ppv) stocks: serum, serum-free, and purified. the flux profile with ppv spiking was similar to that without spiking for normal load filtration of about 250-300 l/m(2) . high volume (3 vol %) of serum-free ppv and 1 vol % serum ppv reduced the flux to some extent for high-load filt ... | 2011 | 21312364 |
| molecular epidemiology of plum pox virus in japan. | for a molecular epidemiological study based on complete genome sequences, 37 plum pox virus (ppv) isolates were collected from the kanto region in japan. pair-wise analyses revealed that all 37 japanese isolates belong to the ppv-d strain, with low genetic diversity (less than 0.8%). in phylogenetic analysis of the ppv-d strain based on complete nucleotide sequences, the relationships of the ppv-d strain were reconstructed with high resolution: at the global level, the american, canadian, and ja ... | 2011 | 21198358 |
| thermal image scanning for influenza border screening: results of an airport screening study. | infrared thermal image scanners (itis) appear an attractive option for the mass screening of travellers for influenza, but there are no published data on their performance in airports. | 2011 | 21245928 |
| false-positive human immunodeficiency virus enzyme immunoassay results in pregnant women. | examine whether false-positive hiv enzyme immunoassay (eia) test results occur more frequently among pregnant women than among women who are not pregnant and men (others). | 2011 | 21304592 |
| performance of the aspartate aminotransferase-to-platelet ratio index for the staging of hepatitis c-related fibrosis: an updated meta-analysis. | the aspartate aminotransferase-to-platelet ratio index (apri), a tool with limited expense and widespread availability, is a promising noninvasive alternative to liver biopsy for detecting hepatic fibrosis. the objective of this study was to update the 2007 meta-analysis to systematically assess the accuracy of apri in predicting significant fibrosis, severe fibrosis, and cirrhosis stage in hepatitis c virus (hcv) monoinfected and hcv / human immunodeficiency virus (hiv) coinfected individuals. ... | 2011 | 21319189 |
| validity of two rapid point of care influenza tests and direct fluorecence assay in comparison of real time pcr for swine of origin influenza virus. | a novel swine origin influenza virus (s-oiv) is continue to spread worldwide and a global declaration of 2009 influenza pandemic was made by world health organization (who) june 2009, this along with approaching the winter season at the northern hemisphere, increase the interest to provide a quick, easy, affordable and available point of care testing for s-oiv. | 2011 | 21338954 |
| twelve week post-treatment follow-up predicts sustained virological response to pegylated interferon and ribavirin therapy in hiv/hepatitis c virus co-infected patients. | the aim of this study was to evaluate whether the assessment of hepatitis c virus (hcv) rna serum at 12 weeks after the end of treatment (w12) was as informative as after 24 weeks (w24) for determining sustained virological response (svr) in hiv/hcv co-infected patients who received a combination of pegylated interferon (peg-inf) plus ribavirin (peg-inf/rbv) and had a virological response at the end of treatment. | 2011 | 21415037 |
| pneumococcal nasopharyngeal carriage among infants born to human immunodeficiency virus-infected mothers immunized with pneumococcal polysaccharide vaccine during gestation. | we have previously shown that 23-valent pneumococcal polysaccharide vaccine (ppv) is immunogenic in human immunodeficiency virus (hiv)-infected mothers and provides vaccine-induced antibodies to the infant. we compared the nasopharyngeal pneumococcal colonization (npc) rates in <6-month-old infants born to hiv-infected mothers, according to immunization with ppv during pregnancy. | 2011 | 21200361 |
| molecular, ultrastructural, and biological characterization of pennsylvania isolates of plum pox virus. | plum pox virus (ppv) was identified in pennsylvania in 1999. the outbreak was limited to a four-county region in southern pennsylvania. initial serological and molecular characterization indicated that the isolates in pennsylvania belong to the d strain of ppv. the pennsylvania isolates were characterized by sequence analysis, electron microscopy, host range, and vector transmission to determine how these isolates related to their previously studied european counterparts. genetically, pennsylvan ... | 2011 | 21261466 |
| mediterranean and central-eastern european countries host viruses of two different clades of plum pox virus strain m. | the genetic diversity of plum pox virus strain m (ppv-m) was assessed by analyzing 28 isolates collected in 8 european countries. two genomic fragments spanning the (cter)p3-6k1-(nter)ci coding region as well as the full coat protein coding region were sequenced directly from pcr products. phylogenetic analysis showed that the geographical origin of the collected isolates was clearly associated with two different ppv-m clades. moreover, the pattern of substitutions in the cp gene shed light on t ... | 2011 | 21293968 |
| sequence variability, recombination analysis, and specific detection of the w strain of plum pox virus. | plum pox virus (ppv), a member of the genus potyvirus, is the causal agent of sharka, the most detrimental disease of stone-fruit trees worldwide. ppv isolates are grouped into seven distinct strains. the minor ppv-w strain was established recently for the divergent w3174 isolate found in canada. here, the partial or complete genomic sequences of four ppv-w isolates from latvia have been determined. the completely sequenced isolates lv-141pl and lv-145bt share 93.1 and 92.1% nucleotide identity, ... | 2011 | 21425932 |
| [coinfection effects of porcine circovirus type 2 and porcine parvovirus in vivo on phagocytosis and interferon mrna expression of porcine alveolar macrophages]. | our study is to analyse the coinfection effects of porcine circovirus type 2 (pcv2) and porcine parvovirus (ppv) in vivo on phagocytosis and interferon mrna expression level of porcine alveolar macrophages (pam). | 2011 | 21465796 |
| the immune enhancement of propolis adjuvant on inactivated porcine parvovirus vaccine in guinea pig. | two experiments were carried out. in immune response test, the immune enhancement of propolis, oilemulsion and aluminium salt were compared in guinea pig vaccinated with inactivated porcine parvovirus (ppv) vaccine. the result showed that three adjuvants could enhance antibody titer, t lymphocyte proliferation, il-2 and il-4 secretion of splenic lymphocyte. the action of propolis was similar to that of oilemulsion and superior to that of aluminium salt, especially in early period of vaccination ... | 2011 | 21482422 |
| field effectiveness of pandemic and 2009-2010 seasonal vaccines against 2009-2010 a(h1n1) influenza: estimations from surveillance data in france. | in this study, we assess how effective pandemic and trivalent 2009-2010 seasonal vaccines were in preventing influenza-like illness (ili) during the 2009 a(h1n1) pandemic in france. we also compare vaccine effectiveness against ili versus laboratory-confirmed pandemic a(h1n1) influenza, and assess the possible bias caused by using non-specific endpoints and observational data. | 2011 | 21573005 |
| intestinal gene expression in pigs experimentally co-infected with pcv2 and ppv. | the objective of the present study was to characterize the local immune reaction in the intestine of pigs experimentally infected with pcv2 and ppv. archived intestinal material from an experimental study in which pigs were co-infected with a swedish isolate of pcv2 (s-pcv2) and ppv, or a reference isolate of pcv2 (pcv2-1010) and ppv, were used. the intestinal samples were analysed by qpcr for expression of a number of selected cytokines and the overall gene expression in the intestine was scree ... | 2011 | 21605916 |
| the rna profile of porcine parvovirus 4, a boca-like virus, is unique among the parvoviruses. | ppv4 transcribes its genome from a single promoter, and the rnas are generated via alternate splicing coupled with alternate polyadenylation, a strategy similar to that of the bocaviruses; however, several differences were detected. the ppv4 orf1 codes for four ns proteins, while the bocavirus orf1 codes for 1-3 ns proteins. whereas the vp1/vp2 capsid proteins of bocavirus are encoded by a single rna, vp1 and vp2 of ppv4 are encoded by two separate rnas. while orf3 of ppv4 encodes two np protein ... | 2011 | 21785854 |
| magnetic resonance elastography and biomarkers to assess fibrosis from recurrent hepatitis c in liver transplant recipients. | background.: imaging techniques evaluating liver stiffness (magnetic resonance elastography [mre]) and biomarkers may be useful indicators of fibrosis stage in hepatitis c virus (hcv)+patients. our aim was to compare the accuracy of mre and biomarkers in staging fibrosis because of recurrent hcv in liver transplant (lt) recipients with hepatocellular carcinoma. methods.: liver magnetic resonance imaging and mre, fibrospectii, aspartate aminotransferase-to-platelet ratio index (aspartate aminotra ... | 2011 | 21822174 |
| optimal follow-up time to determine the sustained virological response in patients with chronic hepatitis c receiving peg-ifn and ribavirin. | background: this study evaluated whether the assessment of hcv-rna at 12 weeks (fw+12) post treatment follow-up was as applicable as fw+24 to evaluate sustained virological response (svr) using the highly sensitive real-time pcr hcv assay. methods and results: two hundred twenty-two patients with chronic hepatitis c were included in this study. pegylated interferon (peg-ifn) and ribavirin were administered for 24-72 weeks based on the genotype and viral load. serum hcv-rna was measured using rea ... | 2011 | 21649727 |
| a live-attenuated chimeric porcine circovirus type 2 (pcv2) vaccine is transmitted to contact pigs but is not upregulated by concurrent infection with porcine parvovirus (ppv) and porcine reproductive and respiratory syndrome virus (prrsv) and is efficacious in a pcv2b-prrsv-ppv challenge model. | the live chimeric porcine circovirus type 2 (pcv2) vaccine with the capsid gene of the emerging subtype 2b cloned in the genomic backbone of the nonpathogenic pcv1 is attenuated in vivo and induces protective immunity against pcv2. to further determine the safety and efficacy of this experimental vaccine, we tested for evidence of pig-to-pig transmission by commingling nonvaccinated and vaccinated pigs, determined potential upregulation by simultaneous vaccination and infection with porcine parv ... | 2011 | 21653745 |
| visualization of plant viral suppressor silencing activity in intact leaf lamina by quantitative fluorescent imaging. | abstract: background: transient expression of proteins in plants has become a favoured method over the production of stably transformed plants because, in addition to enabling high protein yields, it is both fast and easy to apply. an enhancement of transient protein expression can be achieved by plant virus-encoded rna silencing suppressor proteins. since viral suppressor proteins differ in their efficiency to enhance transient protein expression in plants, we developed a whole-leaf green fluor ... | 2011 | 21812965 |
| porcine parvovirus removal using trimer and biased hexamer peptides. | assuring the microbiological safety of biological therapeutics remains an important concern. our group has recently reported small trimeric peptides that have the ability to bind and remove a model non-enveloped virus, porcine parvovirus (ppv), from complex solutions containing human blood plasma. in an effort to improve the removal efficiency of these small peptides, we created a biased library of hexamer peptides that contain two previously reported trimeric peptides designated wrw and kyy. th ... | 2011 | 21751387 |
| phylogenetic analysis of porcine parvoviruses from swine samples in china. | abstract: | 2011 | 21703005 |
| A novel recombinant pseudorabies virus expressing parvovirus VP2 gene: Immunogenicity and protective efficacy in swine. | Porcine parvovirus (PPV) VP2 gene has been successfully expressed in many expression systems resulting in self-assembly of virus-like particles (VLPs) with similar morphology to the native capsid. Here, a pseudorabies virus (PRV) system was adopted to express the PPV VP2 gene. | 2011 | 21679423 |
| noninvasive diagnostic criteria for hepatocellular carcinoma in hepatic masses >2 cm in a hepatitis b virus-endemic area. | background: noninvasive criteria for diagnosing hepatocellular carcinoma (hcc) suggested by the american association for the study of liver diseases (aasld) in 2005 consisted of serum a-fetoprotein (afp) level >200 ng/ml or a typical enhancement pattern (arterial enhancement and portal/delayed washed out) on dynamic imaging of hepatic mass(es) >2 cm in a cirrhotic liver. aims: to validate these criteria in a korean population and to evaluate whether these criteria are applicable to patients with ... | 2011 | 21745284 |
| genetically engineered resistance to plum pox virus infection in herbaceous and stone fruit hosts. | plum pox virus (ppv), a potyvirus, is the causal agent of sharka, the most detrimental viral disease affecting stone fruittrees. this review focuses on research carried out to obtain ppv- resistant transgenic plants and on how biotechnological strategies evolved in light of the scientific advances made during the last several years. successful rna silencing strategies that confer high level of resistance to strains of ppv have been developed and tested under laboratory and greenhouse conditions. ... | 2011 | 21844696 |
| narrowing down the apricot plum pox virus resistance locus and comparative analysis with the peach genome syntenic region. | sharka disease, caused by the plum pox virus (ppv), is one of the main limiting factors for stone fruit crops worldwide. only a few resistance sources have been found in apricot (prunus armeniaca l.), and most studies have located a major ppv resistance locus (ppvres) on linkage group 1 (lg1). however, the mapping accuracy was not sufficiently reliable and ppvres was predicted within a low confidence interval. in this study, we have constructed two high-density simple sequence repeat (ssr) impro ... | 2011 | 21722293 |
| porcine bocaviruses: genetic analysis and prevalence in chinese swine population. | summaryin members of the bocavirus genus, that contain three open reading frames (orfs) of the parvovirinae subfamily, porcine bocaviruses (pobovs) exhibit the most genetic diversity. based on the orf2-encoded viral protein (vp1) classification, the six reported porcine bocaviruses were grouped into four species: pobov1 (porcine boca-like virus or pbolv), pobov2 (porcine parvovirus 4 or ppv4), pobov3 (pbov1/pbov2) and pobov4 (6v/7v), with pobov3 and pobov4 each having two genotype viruses. all f ... | 2011 | 21676363 |
| in vitro evaluation of antiviral and virucidal activity of a high molecular weight hyaluronic acid. | hyaluronic acid (ha), a non-sulphated glycosaminoglycan, is present in synovial fluid, vitreous humour serum and many connective tissues. pharmaceutical preparations of ha are used in clinical practice for wound healing, joint pain, kerato-conjunctivitis, asthma, mouth care, oesophageal-reflux, and gastritis. moreover, it is used as a filler to counteract ageing and facial lipoatrophy. our study aims at investigating the in vitro antiviral activity of a high molecular weight ha. | 2011 | 21439070 |
| Prediction of sustained response to low-dose pegylated interferon alpha-2b plus ribavirin in patients with genotype 1b and high hepatitis C virus level using viral reduction within 2 weeks after therapy initiation. | Aim: Continuation of pegylated interferon (PEG-IFN) plus ribavirin at the recommended dose is difficult in elderly patients and/or patients with cytopenia or complications. Whether the therapeutic efficacy of low-dose PEG-IFN plus ribavirin therapy could be predicted based on virological response within 2 weeks of therapy initiation was evaluated. Methods: A total of 106 patients with a high viral load of genotype-1b hepatitis C virus (HCV) underwent low-dose PEG-IFN plus ribavirin therapy. PE ... | 2011 | 21951330 |
| comparison of the immune responses induced by oral immunization of mice with lactobacillus casei-expressing porcine parvovirus vp2 and vp2 fused to escherichia coli heat-labile enterotoxin b subunit protein. | the major structural protein vp2 of porcine parvovirus (ppv) was used as the model parvovirus antigen, which has been expressed in lactobacillus casei fusing with escherichia coli heat-labile enterotoxin b subunit (ltb) as mucosal adjuvant. the vp2-ltb dna fragment was cloned into vector ppg611 or ppg612 to generated inducible surface-displayed and secretion expression systems based on xylose promoter, designated as rlc:ppg611-vp2-ltb (recombinant l. casei) and rlc:ppg612-vp2-ltb, respectively. ... | 2011 | 20226529 |
| combined use of aspartate aminotransferase, platelet count and matrix metalloproteinase 2 measurements to predict liver fibrosis in hiv/hepatitis c virus-coinfected patients. | noninvasive tests that can be used in place of liver biopsy to diagnose fibrosis have major limitations. they either leave a significant proportion of patients without a definitive diagnosis or produce inaccurate results. moreover, the performance of these tests is lower in hiv/hepatitis c virus (hcv) coinfection. against this background, we examined the utility of serum matrix metalloproteinase 2 (mmp-2) and tissue inhibitor of metalloproteinase 1 (timp-1) measurements in combination with routi ... | 2011 | 20497249 |
| o-glcnacylation of the plum pox virus capsid protein catalyzed by secret agent: characterization of o-glcnac sites by electron transfer dissociation mass spectrometry. | the capsid protein of plum pox virus (ppv-cp) is modified with o-linked β-n-acetylglucosamine (o-glcnac). in arabidopsis thaliana this modification is made by an o-glcnac transferase named secret agent (sec). modification of ppv-cp by sec is hypothesized to have a direct role in the infection process, because virus titer and rate of spread are reduced in sec mutants. previous studies used deletion mapping and site-directed mutagenesis to identify four o-glcnac sites on the capsid protein that ar ... | 2011 | 20676902 |
| loop-mediated isothermal amplification for rapid detection and differentiation of wild-type pseudorabies and gene-deleted virus vaccines. | a loop-mediated isothermal amplification (lamp) assay was developed specifically for detection and differentiation of pseudorabies virus (prv). one group of primers was designed to detect wild-type strains (i.e., strains with the ge gene) and the other group of primers was designed to detect both prv ge-vaccine and wild-type strains (i.e., strains with the gg gene and with or without the ge gene). after amplification by bst enzyme at a constant temperature of 65 degrees c, a laddering of bright ... | 2010 | 20691214 |
| the chemokine cxcl13 is a prognostic marker in clinically isolated syndrome (cis). | there is increasing recognition of the importance of b lymphocytes in the immunopathogenesis of multiple sclerosis (ms), encouraging the evaluation of b cell-associated biomarkers in the cerebrospinal fluid (csf). we aimed to evaluate the relevance of the b cell chemoattractant cxcl13 as a prognostic marker in patients with clinically isolated syndrome (cis) regarding conversion to ms, and to compare it to barkhof criteria in magnetic resonance imaging (mri), oligoclonal bands (ocb) and the poly ... | 2010 | 20700489 |
| audit of paired anal cytology and histopathology outcomes in patients referred to a public sexual health clinic. | the level of agreement between anal cytology and histopathology is not clear with only a few studies evaluating the reliability of anal specimen reporting. australian data in relation to this are limited. | 2010 | 20719226 |
| a randomized clinical trial comparing revaccination with pneumococcal conjugate vaccine to polysaccharide vaccine among hiv-infected adults. | the risk of pneumococcal disease persists, and antibody responses to revaccination with the 23-valent polysaccharide vaccine (ppv) are low among human immunodeficiency virus (hiv)-infected adults. we determined whether revaccination with the 7-valent pneumococcal conjugate vaccine (pcv) would enhance these responses. | 2010 | 20795819 |
| quantitative serum hbsag and hbeag are strong predictors of sustained hbeag seroconversion to pegylated interferon alfa-2b in hbeag-positive patients. | to evaluate the usefulness of quantitative hepatitis b surface antigen (hbsag) and hepatitis b e antigen (hbeag) for predicting hbeag seroconversion in chronic hepatitis b patients treated with conventional interferon (ifn) alfa-2b or pegifn alfa-2b. | 2010 | 20796146 |
| content of phenolic compounds and antioxidant capacity in fruits of apricot genotypes. | research on natural compounds is increasingly focused on their effects on human health. in this study, we were interested in the evaluation of nutritional value expressed as content of total phenolic compounds and antioxidant capacity of new apricot (prunus armeniaca l.) genotypes resistant against plum pox virus (ppv) cultivated on department of fruit growing of mendel university in brno. fruits of twenty one apricot genotypes were collected at the onset of consumption ripeness. antioxidant cap ... | 2010 | 20877223 |
| silencing of plum pox virus 5'utr/p1 sequence confers resistance to a wide range of ppv strains. | an effective disease-control strategy should protect the host from the major economically important and geographically widespread variants of a pathogen. plum pox virus (ppv) is the causal agent of sharka, the most devastating viral disease of prunus species. we have shown previously that the hairpin rna expression driven by h-utr/p1, h-p1/hcpro, h-hcpro and h-hcpro/p3 constructs, derived from the ppv-m ispave44 isolate, confers resistance to the homologous virus in nicotiana benthamiana plants. ... | 2010 | 20963442 |
| [construction and immunogenicity of recombinant porcine parvovirus-like particles with somatostatin]. | in order to obtain a virus-like particle vaccine both for porcine parvovirus (ppv) prevention and growth-promotion, vp2 gene of ppv nj-a strain was amplified with pcr, and four copies of synthetic somatostatin gene were fused to the n-terminal of vp2 gene. the fused gene was cloned into pfast-ht a to construct the recombinant plasmid pfast-ss4-vp2, then the pfast-ss4-vp2 was transformed into dh10bac competent cells and recombined with shuttle vector bacmid, followed by identification with blue-w ... | 2010 | 21090109 |
| detection of a novel porcine parvovirus, ppv4, in chinese swine herds. | to determine whether the novel porcine parvovirus type 4 (ppv4) recently reported in america is prevalent in china, a set of specific primers was designed and used for molecular survey of ppv4 among the clinical samples collected from various provinces of china between 2006 and 2010. the results showed that ppv4 is present in chinese swine herds at a rate of 2.09% (12/573) among the clinical samples examined and 0.76% (1/132) among the samples taken from healthy animals. we also noted that ppv4 ... | 2010 | 21092136 |
| detection of porcine parvovirus using a taqman-based real-time pcr with primers and probe designed for the ns1 gene. | a taqman-based real-time polymerase chain reaction (pcr) assay was devised for the detection of porcine parvovirus (ppv). two primers and a taqman probe for the non-structural protein ns1 gene were designed. the detection limit was 1 x 10² dna copies/μl, and the assay was linear in the range of 1 x 10² to 1 x 10⁹ copies/μl. there was no cross-reaction with porcine circovirus 2 (pcv2), porcine reproductive and respiratory syndrome virus (prrsv), pseudorabies virus (prv), classical swine fever vir ... | 2010 | 21126330 |
| immunopathological characterization of porcine circovirus type 2 infection-associated follicular changes in inguinal lymph nodes using high-throughput tissue microarray. | the immunopathogenesis of porcine circovirus type 2 (pcv2) infection in conventional pigs is complicated by various environmental factors and individual variation and is difficult to be completely reproduced experimentally. in the present field-based study, a tissue microarray (tma) consisting of a series of lymphoid follicles having different pcv2-loads was constructed using formalin-fixed and paraffin-embedded superficial inguinal lymph nodes (lns) from 102 pigs. using the tma, a wide range of ... | 2010 | 21126833 |
| role of simple biomarkers in predicting fibrosis progression in hcv infection. | to examine the accuracy of the aspartate aminotransferase (ast)/platelet ratio index (apri) and fib-4, in predicting longitudinal changes in liver histology in hepatitis c virus (hcv) patients. | 2010 | 21128320 |
| production and purification of vp2 protein of porcine parvovirus expressed in an insect-baculovirus cell system. | the porcine parvovirus (ppv) vp2 protein was expressed in an insect-baculovirus cell system and was purified using ni-nta affinity column chromatography. the recombinant 6-his-tagged vp2 protein with molecular mass (mr) of about 64 kda was detected by anti-his antibody and anti-ppv serum. electron microscopy showed that the purified vp2 protein assembled into spherical particles with diameters ranging from 20 to 22 nm. the expressed vp2 was antigenically similar to the native capsid protein acco ... | 2010 | 21143963 |
| the diagnostic and prognostic significance of soluble urokinase plasminogen activator receptor in crimean-congo hemorrhagic fever. | crimean-congo hemorrhagic fever (cchf) is a potentially fatal disease caused by a tick-borne virus from the bunyaviridae family. it has recently been reported that soluble urokinase-type plasminogen activator receptor (supar), secreted from endothelial cells and the mononuclear phagocyte system, one of the main targets of the cchf virus, is a potential biomarker for several bacterial and viral infection diseases. | 2010 | 21167774 |
| [combined detection of epstein-barr virus antibodies for serodiagnosis of nasopharyngeal carcinoma]. | to investigate the value of combined detection of epstein-barr virus (ebv) vca/iga, ea/iga, rta/igg and ebna1/iga in serodiagnosis of nasopharyngeal carcinoma (npc). | 2010 | 21177196 |
| improving the immunogenicity of pneumococcal conjugate vaccine in hiv-infected adults with a toll-like receptor 9 agonist adjuvant: a randomized, controlled trial. | persons infected with human immunodeficiency virus (hiv) are often hyporesponsive to immunization, including pneumococcal vaccines. we hypothesized that adding cpg 7909, a toll-like receptor 9 (tlr9) agonist and vaccine adjuvant, to 7-valent pneumococcal conjugate vaccine (7vpnc) would increase its immunogenicity in hiv-infected adults. | 2010 | 20504165 |
| studies of porcine circovirus type 2, porcine boca-like virus and torque teno virus indicate the presence of multiple viral infections in postweaning multisystemic wasting syndrome pigs. | in a previous study, using random amplification and large-scale sequencing technology, we identified a novel porcine parvovirus belonging to the genus bocavirus in the background of porcine circovirus type 2 (pcv-2) in swedish pigs with postweaning multisystemic wasting syndrome (pmws). in addition to bocavirus we demonstrated the presence of torque teno virus (ttv) genogroups 1 and 2 in these cases of pmws, indicating the simultaneous presence of several viruses in this disease complex. in the ... | 2010 | 20542066 |
| selected swine viral pathogens in indoor pigs in spain. seroprevalence and farm-level characteristics. | a serosurvey on porcine reproductive and respiratory syndrome virus (prrsv), swine influenza virus (siv), aujeszky's disease virus ge protein (adv ge), porcine parvovirus (ppv) and porcine circovirus type 2 (pcv2) was carried out in spanish pig herds. the serosurvey consisted of two studies. first, a retrospective study assessed the proportion of seropositive boar, sow and fattening pig herds and their seroprevalences to prrsv, siv, adv ge and ppv from 2003 to 2005 and to pcv2 from 2000 to 2005. ... | 2010 | 20569418 |
| [rapid influenza diagnostic tests for detection of novel influenza a (h1n1) virus in children]. | accuracy of rapid bd directigen(®) ez flu a+b diagnostic test against influenza a (h1n1) virus in children. | 2010 | 20579777 |
| prevalence of antibodies to selected viral and bacterial pathogens in wild boar (sus scrofa) in campania region, italy. | serum samples were collected from wild boars (sus scrofa) harvested during the 2005-2006 hunting season in campania, southern italy. samples were tested for antibodies to leptospira interrogan, brucella spp., salmonella spp., aujeszky disease virus (adv), porcine reproductive and respiratory stress syndrome virus (prrsv), porcine parvovirus (ppv), classical swine fever virus (csfv), and swine vesicular disease virus (svdv). of the 342 serum samples tested, 15 (4.4%) were seropositive to brucella ... | 2010 | 20090052 |
| the genome of pseudocowpoxvirus: comparison of a reindeer isolate and a reference strain. | parapoxviruses (ppv), of the family poxviridae, cause a pustular cutaneous disease in sheep and goats (orf virus, orfv) and cattle (pseudocowpoxvirus, pcpv and bovine papular stomatitis virus, bpsv). here, we present the first genomic sequence of a reference strain of pcpv (vr634) along with the genomic sequence of a ppv (f00.120r) isolated in finland from reindeer (rangifer tarandus tarandus). the f00.120r and vr634 genomes are 135 and 145 kb in length and contain 131 and 134 putative genes, re ... | 2010 | 20107016 |
| [association between igg antibody against the c-terminal region of the pres1 protein of hepatitis b virus and the early response to interferon therapy in chronic hepatitis b]. | to determine the relationship between igg antibody against the c-terminal region of the pres1 protein of hepatitis b virus and the early response to interferon therapy in chronic hepatitis b. | 2010 | 20128959 |
| colostral transmission of porcine circovirus 2 (pcv-2): reproduction of post-weaning multisystemic wasting syndrome in pigs fed milk from pcv-2-infected sows with post-natal porcine parvovirus infection or immunostimulation. | post-weaning multisystemic wasting syndrome (pmws) was reproduced in pigs fed colostrum and milk from porcine circovirus 2 (pcv-2)-infected sows and infected post-natally with porcine parvovirus (ppv) or immunostimulated. pregnant sows were inoculated intranasally with either pcv-2 (n=5) or pcv-2-free pk-15 cell lysates (control, n=10) 3 weeks before the expected farrowing date. newborn piglets from five of the control sows were introduced to pcv-2-infected sows (n=6 for each sow) and allowed to ... | 2010 | 20147521 |
| ykl-40 and hyaluronic acid (ha) as noninvasive markers of liver fibrosis in kidney transplant patients with hcv chronic infection. | hepatitis c is highly prevalent among kidney transplant (kt) recipients. in this population, the natural history of hepatitis c virus (hcv) infection and its proper management remains controversial. the invasiveness of the procedure and the interpretation variability of liver biopsy limit its use in these patients. we sought to evaluate the performance of ykl-40 and ha as markers of liver fibrosis in kt patients with hcv infection. | 2010 | 20163287 |
| use of simple noninvasive biomarkers to predict liver fibrosis in hiv/hcv coinfection in routine clinical practice. | simple noninvasive tests to predict fibrosis, as an alternative to liver biopsy (lb), are needed. of these, the aspartate aminotransferase (ast) to platelet ratio index (apri) and the forns index (fi) have been validated in hiv/hepatitis c virus (hcv) coinfection. however, these indexes may have lower diagnostic value in situations other than the circumscribed conditions of validation studies. we therefore examined the value of the apri and fi in hiv/hcv-coinfected patients for the detection of ... | 2010 | 20180868 |
| [evaluation of the virus-elimination efficacy of nanofiltration (viresolve nfp) for the parvovirus b19 and hepatitis a virus]. | the safety of plasma derivatives has been reinforced since 1980s by variable pathogen inactivation or elimination techniques. nucleic acid amplification test (nat) for the source plasma has also been implemented worldwide. recently nanofiltration has been used in some country for ensuring safety of plasma derivatives to eliminate non-enveloped viruses such as parvovirus b19 (b19v) and hepatitis a virus (hav). we evaluated the efficacy of nanofiltration for the elimination of b19v and hav. | 2010 | 20197722 |
| on-treatment serum hbsag level is predictive of sustained off-treatment virologic response to telbivudine in hbeag-positive chronic hepatitis b patients. | effective management of chronic hepatitis b infection is still very challenging, despite decades of clinical research. telbivudine is one of the most frequently used antiviral drug at the current stage, but its long-term effectiveness, particularly at off-treatment, is still unclear. | 2010 | 20233672 |
| identification and molecular cloning of a novel porcine parvovirus. | a novel porcine parvovirus, ppv4, was identified in the lung lavage of a diseased pig coinfected with porcine circovirus type 2. this virus exhibits limited similarity to its closest relative, bovine parvovirus 2, but resembles viruses of the genus bocavirus (bovine parvovirus, canine minute virus and human bocavirus) that encode an additional orf3. the orf3 of ppv4 is predicted to encode a protein of 204 amino acid residues, which is similar in size to the orf3-encoded proteins of the bocavirus ... | 2010 | 20339886 |
| fat mass ratio: an objective tool to define lipodystrophy in hiv-infected patients under antiretroviral therapy. | human immunodeficiency virus (hiv) infection and its treatment with antiretroviral therapy (art) have been associated with lipodystrophy. different clinical methodologies have been used to define the syndrome. the aim of this study was to propose gender-specific reference values using objective measurements for defining lipodystrophy in hiv-infected patients. using dual-energy x-ray absorptiometry (dxa), total body composition was analyzed in 221 hiv-infected patients under art (146 men). we use ... | 2010 | 20347364 |
| correlation between the composition of multivalent antibody conjugates with colloidal gold nanoparticles and their affinity. | interactions between multivalent preparations of antibodies (conjugated with colloidal gold nanoparticles (gnp) as a carrier system) and a multivalent ligand were investigated. the aim of the present study was to reveal the relationship between the affinity of the conjugate and its composition (i.e., the valency). surface plasmon resonance was applied to study the affinity and the kinetics of the interaction of multivalent conjugates and multivalent virus (on the example of the plum pox virus (p ... | 2010 | 20347832 |
| assessment of alpha-fetoprotein in the diagnosis of hepatocellular carcinoma in middle eastern patients. | alpha-fetoprotein (afp) levels for the diagnosis of hepatocellular carcinoma (hcc) may vary by geographical region and racial background. no data exists for this test in the middle eastern population. in addition, there is limited data on the impact of virological status on afp levels. | 2010 | 20397051 |
| diagnosis and phylogenetic analysis of orf virus from goats in china: a case report. | orf virus (orfv) is the etiological agent of contagious pustular dermatitis and is the prototype of the genus parapoxvirus (ppv). it causes a severe exanthematous dermatitis that afflicts domestic and wild small ruminants. | 2010 | 20416112 |
| comparison of immunohistochemistry and polymerase chain reaction for detection of west nile virus in naturally infected dead birds. | credible vector-borne disease surveillance programs, especially in developing countries with limited resources, must include diagnostic tests that are efficient, inexpensive and simple and safe to administer while maintaining high levels of sensitivity and specificity. since immunohistochemistry (ihc) includes most of these features, its sensitivity, specificity, predictive positive value (ppv) and predictive negative value (pnv) for west nile virus (wnv) screening were compared to those of the ... | 2010 | 21045374 |
| a single amino acid mutation alters the capsid protein electrophoretic double-band phenotype of the plum pox virus strain ppv-rec. | plum pox virus (ppv) isolates differ by their capsid protein (cp) mobility in sds-page. these electrophoretic phenotypes are likely to result from post-translational modifications of the cp. we demonstrated that the cp mobility was solely determined by the cp n-terminal region. sequence comparison pinpointed a possible role of mutations at position 66 in determining the cp phenotype of ppv-rec isolates. site-directed mutagenesis of a chimeric clone demonstrated that gly(66) in the cp resulted in ... | 2010 | 20422234 |
| the need for culture collections to support plant pathogen diagnostic networks. | plant-pathogenic microorganisms, by virtue of their size, similarity in disease symptoms and closely related morphologies, are notoriously difficult to diagnose and detect. diagnosis gives proof as to the causal agent of disease and is important for developing appropriate control measures. detection shows the presence of a microorganism and is of importance for safeguarding national and international trade. live reference collections are required to characterize the taxonomy and function of micr ... | 2010 | 20457251 |
| multiple pathways involved in porcine parvovirus cellular entry and trafficking toward the nucleus. | porcine parvovirus (ppv) is a major cause of reproductive failure in swine. the mechanisms implicated in the first steps of infection that lead to the delivery of the ppv genome to the nucleus are poorly understood. in the present work, a panel of chemical inhibitors was used to dissect the cellular mechanisms involved in establishing a ppv infection. the results demonstrated that following binding to sialic acids on cell surface glycoproteins, the virus used both clathrin-mediated endocytosis a ... | 2010 | 20484503 |
| simultaneous detection of porcine circovirus type 2, classical swine fever virus, porcine parvovirus and porcine reproductive and respiratory syndrome virus in pigs by multiplex polymerase chain reaction. | a multiplex polymerase chain reaction (pcr) was designed for the simultaneous detection of four viruses involved in reproductive and respiratory failure in pigs: porcine circovirus type 2 (pcv-2), porcine parvovirus (ppv), classical swine fever virus (csfv) and porcine reproductive and respiratory syndrome virus (prrsv). each of the four pairs of oligonucleotide primers exclusively amplified the targeted fragment of the specific viruses. the sensitivity of the multiplex pcr using purified plasmi ... | 2010 | 19131259 |
| development and validation of reverse transcription loop-mediated isothermal amplification for detection of prrsv. | to establish a reverse-transcription loop-mediated isothermal amplification (rt-lamp) method for rapid detection of porcine reproductive and respiratory syndrome virus (prrsv), four primers specific to six regions of the n gene were designed. after amplification in an isothermal water bath for 1 h, samples containing prrsv generated the expected ladder-like products while porcine parvovirus, porcine circovirus, classic swine fever virus, pseudorabies virus, and swine testis cells generated no pr ... | 2010 | 19911264 |
| chances and limitations of non-invasive tests in the assessment of liver fibrosis in liver transplant patients. | because fibrosis progression resulting in liver cirrhosis represents the main reason for graft lost in patients after liver transplantation, an early detection of liver fibrosis is crucial. in recent years, several non-invasive tests for the assessment of liver fibrosis have been developed. we prospectively assessed the stage of liver fibrosis of 135 liver transplant patients (94 hepatitis c virus [hcv], 41 alcoholic cirrhosis) using liver biopsy, transient elastography, and serum markers. in th ... | 2010 | 19925459 |
| development and evaluation of rapid detection of classical swine fever virus by reverse transcription loop-mediated isothermal amplification (rt-lamp). | a one-step reverse transcription loop-mediated isothermal amplification (rt-lamp) was developed for the detection of classical swine fever virus (csfv) and a set of primers designed based on the e2 gene reference sequences of the csfv. the assay was optimized to amplify csfv rna by incubation at 63 degrees c for 50 min. the rt-lamp amplification products had a ladder-like appearance when electrophoresed on an agarose gel. the rt-lamp assay showed higher sensitivity than the conventional rt-pcr, ... | 2010 | 19931578 |
| identification and phylogenetic analysis of an orf virus isolated from an outbreak in sheep in the jilin province of china. | this study investigated an acute outbreak of contagious ecthyma dermatitis that occurred in november 2008 in a herd of 180 small-tailed han sheep in the jilin province of china. the pathological findings of this case revealed severe vascular proliferation, viral cytopathic changes in keratinocytes by vacuolar degeneration, ballooning degeneration, and eosinophilic cytoplasmic inclusions, suggesting that the disease could have been caused by an orf virus (orfv) infection. immunohistochemistry, in ... | 2010 | 19948384 |
| enhancing the scope of conventional cervical cytology for detecting hpv infection. | cervical neoplasia is attributed to a persistent human papilloma virus infection. the pap smear being the mainstay of cervical cancer screening in low-resource settings, we studied the nonclassical features which might indicate hpv infection. these included abortive koilocytes, mild dyskeratosis, parakeratosis, mild nuclear hyperchromasia, bi/multinucleation, measles cells, and keratohyaline-like granules. two hundred and eight women with a satisfactory pap smear and a hybrid capture ii test wer ... | 2010 | 19950400 |
| prediction of significant liver fibrosis in kidney transplant patients with chronic hepatitis c virus infection: the tx-3 index. | hcv infection is highly prevalent among kidney transplant (kt) recipients. the natural history and management of these patients are controversial. we sought to assess the diagnostic value of noninvasive markers of liver fibrosis in kt hcv-infected patients. this cross-sectional study included 102 kt individuals with positive hcv-rna. bivariate and multivariate analyses were used to identify variables associated with significant fibrosis (metavir > or = f2). significant fibrosis was observed in 2 ... | 2010 | 19758273 |
| evaluation of anti-varicella antibody in young women before their marriage: a sero-epidemiologic study in iran. | chickenpox during pregnancy can cause severe complications in both the mother and her baby. however, no complications will occur in a mother with proper immunity. therefore, physicians and health systems can make better decisions when they know the immunologic status of the women in a community. | 2010 | 20538515 |
| atypical glandular cells (agc): thinprep imaging system (tis), manual screening (ms), and correlation with hybrid capture 2 (hc2) hpv dna testing. | the aim of the study was to determine if the thinprep imaging system (t1s) improves the positive predictive value (ppv) of atypical glandular cell (agc) diagnosis for identifying hpv-related squamous and/or glandular lesions over manual screening (ms), and if human papilloma virus (hpv)-dna testing improves the diagnostic yield. | 2010 | 20014311 |