PMID(sorted ascending)
isothermal amplification coupled with rapid flow-through hybridisation for sensitive diagnosis of plum pox virus.a nucleic acid sequence-based amplification method coupled with rapid flow-through hybridisation (nasba-fh) was developed for diagnosis of plum pox virus (ppv). the sensitivity level achieved by nasba-fh was 10 times higher than that obtained by co-pcr and 1000 times higher than the sensitivity afforded by rt-pcr. in addition, samples from 262 stone-fruit trees collected during winter and spring seasons were analysed. these samples were tested using methods recommended by the european and medite ...200717092572
up-regulation of proliferating cell nuclear antigen (pcna) is closely associated with high-risk human papillomavirus (hpv) and progression of cervical intraepithelial neoplasia (cin), but does not predict disease outcome in cervical cancer.proliferating cell nuclear antigen (pcna) is essential for dna replication of mammalian cells and their small dna tumour viruses. the e7 oncoprotein of high-risk human papillomavirus (hpv) is known to activate pcna, shown to be up-regulated in cin and cervical cancer (cc), but still incompletely studied as an intermediate endpoint marker in this disease.200717098349
sensitivity and specificity of current diagnostic tests for gill-associated virus in penaeus monodon.this study reports the sensitivity, specificity, positive predictive value (ppv), negative predictive value (npv) and accuracy between a reverse transcriptase-nested polymerase chain reaction (rt-npcr) and an enzyme-linked immunosorbent assay (elisa) for the detection of gill-associated virus (gav) from a sample of 120 penaeus monodon. subsequently, the same comparisons were applied to the elisa and haemagglutination (ha) assays for detection of gav from a second 120 prawns. the optical density ...200617169111
atypical squamous cells, cannot exclude high grade intraepithelial lesion (asc-h): does hpv matter?the role of human papillomavirus (hpv) in cases diagnosed as atypical squamous cells, cannot exclude high squamous grade intraepithelial lesion (asc-h) in cervical specimens is not well established. the objective of this study is to evaluate the role of hpv status in cases of asc-h in a major cancer center. one hundred thirty-two patients with a diagnosis of asc-h were identified over a 4-yr period in our institution. forty-four of 132 cases were evaluated for high-risk hpv and had biopsy follow ...200717173297
molecular beacon real-time pcr detection of swine viruses.rapid and reliable detection of viral pathogens is critical for the management of the diseases threatening the economic competitiveness of the swine farming industry worldwide. molecular beacon assays are one type of real-time polymerase chain reaction (pcr) technology capable of fast, specific, sensitive, and reliable viral detection. in this paper, the development of molecular beacon assays as novel tools for the rapid detection of aujeszky's disease virus, african swine fever virus, porcine c ...200717196673
study of the virulence and cross-neutralization capability of recent porcine parvovirus field isolates and vaccine viruses in experimentally infected pregnant gilts.the pathogenicity of two recent german field isolates of porcine parvovirus (ppv-27a and ppv-143a) and two vaccine viruses [ppv-nadl-2 and ppv-idt (msv)], which are used for the production of inactivated vaccines, was investigated by inoculation of pregnant sows at day 40 of gestation. post-infection sera of these sows as well as antisera prepared in rabbits by immunization with the four above-mentioned ppv isolates and with the virulent strain ppv-challenge (engl.) were tested for their homolog ...200717251558
variability of parvovirus b19 to inactivation by liquid heating in plasma products.previously, we reported that although human parvovirus b19 in albumin and intravenous immunoglobulin preparations was rapidly inactivated during liquid heating, in contrast to other parvoviruses such as canine parvovirus, sensitivity to heat was highly dependent on the composition of the solution. in this study, we aimed to further elucidate the sensitivity to heat of b19 in haptoglobin and antithrombin (previously named antithrombin iii) preparations during liquid heating.200717298573
immunohistochemical detection of p16ink4a in liquid-based cytology specimens on cell block sections.colposcopy biopsy procedure is a standard recommendation for atypical squamous cell cannot exclude high-grade lesion (asc-h) in abnormal papanicolaou smears. p16 (p16ink4a), a cell cycle regulator, has been shown to be overexpressed in squamous dysplasia. to further improve the diagnostic accuracy of the asc-h papanicolaou smear and to reduce unnecessary procedures, the authors evaluated the utility of immunodetection of p16 in liquid-based cytology specimens on cell blocks.200717330305
vaccination of porcine circovirus type 2 (pcv2)-infected sows against porcine parvovirus (ppv) and erysipelas: effect on post-weaning multisystemic wasting syndrome (pmws) and on pcv2 genome load in the offspring.the effect of different parvovirus+erysipelas vaccination schemes in pcv2-infected sows on pmws outcome in the offspring was investigated under experimental conditions. six pcv2-free sows were first infected oro-nasally with pcv2 two months before insemination (d0; "day 0") and then by the intra-uterine route at insemination (d62). on d21 and d42, vaccinated sows received either the two commercial monovalent vaccines, a1(ppv) and a2(erysipelas), or the bivalent vaccine b (ppv+erysipelas). in add ...200717374380
a delphi exercise used to identify potential causes of variation in litter size of ontario swine.forty-eight people, considered to the swine experts, were asked to collaborate in a delphi exercise to identify the factors which they believed affect litter size in ontario swine. the panel included 16 animal scientists, 16 pork producers, and 16 veterinarians in swine practice. the ten factors with the highest ratings were parity of the sow, mycotoxins in the feed, infections with porcine parvovirus or leptospira spp., breeding gilts on their second versus first observed estrus, the timing of ...199217423928
comparison of the "prodect bcs rv chip" assay with the combination of shell vial cell culture and immunofluorescence antibody test for the detection of respiratory the present study, a multiplex reverse transcriptase polymerase chain reaction combined with a chip hybridization assay (prodect bcs rv chip) was evaluated as an alternative to the combination of immunofluorescent antibody test and shell vial cell culture considered as gold standard for the detection of respiratory viruses. among 100 specimens, 40 were positive using the combination of immunofluorescent antibody test and shell vial cell culture assay in which 9 of them were infected by two di ...200717433455
[prokaryotice expression of the ns1 gene of ppv and renaturation of the recombinant protein].the antigen of ns1 gene of ppv was amplified by pcr, and the amplified fragments were cloned into the prokaryotic expression vector pgex-4t-1. the insert position, the size and the frame were identified by pcr, restriction enzyme digestion and the sequence analysis of the recombinant plasmids. the sequence analysis results of pgex-ns1-hn1 showed that the prokaryotic expression vector was successfully constructed. the target gene was successfully expressed in the host cell bl21 when induced with ...200717436638
[the surface display of porcine parvovirus vp2 protein in lactobacillus casei].lactobacillus casei 393 was selected as a bacterial carrier for the expression of porcine parvovirus (ppv) protective antigen vp2 protein. the gene encoding ppv vp2 protein was cloned into the lactobacillus casei surface expression vector ppg, and then the constructed recombinant vector ppg-vp2 was electrotransformed into lactobacillus casei 393 generating the recombinant system ppg-vp2/l. casei393 expressing ppv vp2 protein. the recombinant strain was induced by 2% lactose in mrs and about 74kd ...200717460908
seroprevalence of varicella antibodies among pregnant women in lyon-france.the purpose of the study was to calculate the seroprevalence of immunity to the varicella-zoster virus (vzv) infection and to evaluate the positive predictive value (ppv) and the negative predictive value (npv) of the self-reported history of vzv infection in pregnant women. a cross sectional study was conducted in 18 private medical analysis laboratories. information on socio-demographic characteristics and past history of varicella or zoster were collected using a questionnaire. blood samples ...200717534728
spatiotemporal patterns and risks of herd breakdowns in pigs with postweaning multisystemic wasting syndrome.a retrospective cohort study of 116 british pig farms was undertaken to investigate the epidemiological risk factors associated with herd breakdowns with postweaning multisystemic wasting syndrome (pmws). farmers reported the pmws status of their herd (case definition 1) and, where applicable, when the disease was first suspected and what they observed; they described a prolonged increase in mortality in six to 16-week-old pigs that was not attributable to any disease known to be on their farm. ...200717545645
simultaneous detection of three porcine viruses by multiplex pcr.specific oligonucleotide primers were selected and combined in a multiplex arrangement, in order to detect simultaneously three economically important porcine viruses by polymerase chain reaction (pcr). the pathogen panel was comprised of viruses that cause reproductive failure in infected herds: aujeszky's disease virus (adv), porcine parvovirus (ppv) and porcine respiratory and reproductive syndrome virus (prrsv). in order to reduce the time required for the detection of the pathogens, the ass ...200717555292
withdrawn: genomic evidence of porcine parvovirus (ppv) in native indian swine.this article has been withdrawn consistent with elsevier policy on article withdrawal ( the publisher apologizes for any inconvenience this may cause.200717560651
efficient priming against classical swine fever with a safe glycoprotein e2 expressing orf virus recombinant (orfv vrv-e2).an increasing demand in livestock animal husbandry for intervention or emergency vaccination strategies requires a rapid onset of protection linked to prevention of infectious agent spread. using the new recombinant parapoxvirus (ppv) orf virus (orfv) as a vaccine expressing the csfv e2 glycoprotein we demonstrate that a single intra-muscular application confers solid protection. in the prime only concept, multi-site application of the vector vaccine turned out to be superior to single-site appl ...200717600594
[monitoring of infectious diseases among wild boars].sixty-seven serum samples and 43 pathological material samples from wild boars, taken in 5 regions of russia and in the kharkov region of the ukraine in 2002 to 2005 were studied. wild boars in some regions of russia were shown to be carriers of aujeszky's disease virus, porcine parvovirus, porcine circovirus type 2, lymphotropic herpesvirus-1, porcine cytomegalovirus, mycoplasma hyopneumoniae and pasteurella multocida. the classical swine fever (csf) virus genome was detected by polymerase chai ...200717601049
assessment of the diversity and dynamics of plum pox virus and aphid populations in transgenic european plums under mediterranean conditions.the molecular variability of plum pox virus (ppv) populations was compared in transgenic european plums (prunus domestica l.) carrying the coat protein (cp) gene of ppv and non-transgenic plums in an experimental orchard in valencia, spain. a major objective of this study was to detect recombination between ppv cp transgene transcripts and infecting ppv rna. additionally, we assessed the number and species of ppv aphid vectors that visited transgenic and non-transgenic plum trees. test trees con ...200817605085
association of myocarditis with high viral load of porcine circovirus type 2 in several tissues in cases of fetal death and high mortality in piglets. a case study.during a period of 1.5 months, a newly established pig herd experienced a high number of mummifications and stillbirths, a high neonatal mortality rate, and many piglets with congenital tremors or hind leg ataxia. after clinical and histological investigations, the submitted animals were divided into 4 groups: mummified or stillborn (n = 6), live born with myocarditis (n = 5) (average age 22.8 days), live born without myocarditis (n = 14) (average age 20.0 days), and control animals from a diffe ...200717609345
validation of a viral and bacterial inactivation step during the extraction and purification process of porcine the last few years, regulations for biomolecule production, and especially for extraction and purification of animal molecules such as collagen, have been reinforced to ensure the sanitary safety of the materials. to be authorized to market biomaterials based on collagen, manufacturers now have to prove that at least one step of their process is described in guidelines to inactivate prion, viruses, and bacteria. the present study focuses on the inactivation step performed during the extractio ...200717611295
evaluation of hepatic steatosis by ultrasound in patients with chronic hepatitis c virus compare two alternative ultrasound parameters, hepatic vein flow (hvf) pattern and presence of focal hypoechoic areas (fha) within the liver hilus, as non-invasive predictors of liver steatosis in patients with chronic hepatitis c virus (hcv) infection.200717617117
clinical utility of afp-l3% measurement in north american patients with hcv-related cirrhosis.lens culinaris agglutinin-reactive fraction of alpha-fetoprotein (afp-l3%) has been reported to be useful in the early detection of hepatocellular carcinoma (hcc) in japan. the aim of this prospective study was to compare the clinical utility of afp-l3% with that of total afp in north american patients.200717617202
a recombinant pseudorabies virus co-expressing capsid proteins precursor p1-2a of fmdv and vp2 protein of porcine parvovirus: a trivalent vaccine candidate.pseudorabies (pr), foot-and-mouth disease (fmd), and porcine parvovirus disease are three important infectious diseases in swine worldwide. the gene-deleted pseudorabies virus (prv) has been used as a live-viral vector to develop multivalent genetic engineering vaccine. in this study, a recombinant prv, which could co-express protein precursor p1-2a of fmdv and vp2 protein of ppv, was constructed using prv tk(-)/ge(-)/lacz(+) mutant as the vector. after homologous recombination and plaque purifi ...200717628752
simple blood tests as noninvasive markers of liver fibrosis in hemodialysis patients with chronic hepatitis c virus infection.hcv infection is common among patients with end-stage renal disease (esrd) on hemodialysis, and it has been considered an independent risk factor for mortality in this setting. although liver biopsy in esrd patients with hcv infection is useful before kidney transplantation, it carries a high risk of complications. we sought to assess the diagnostic value of noninvasive markers to stage liver fibrosis in 203 esrd hcv-infected patients. univariate and multivariate analysis were used to identify v ...200717634962
no influence of collagenous proteins of achilles tendon, skin and cartilage on the virus-inactivating efficacy of peracetic acid-ethanol.the risk of transmitting human pathogenic viruses via allogeneic musculoskeletal tissue transplants is a problem requiring effective inactivation procedures. virus safety of bone transplants was achieved using peracetic acid (paa)-ethanol sterilisation. proteins are known to have an adverse effect on the virus-inactivating capacity of paa. therefore we investigated virus inactivation by paa in collagenous tissues. achilles tendon, skin and cartilage were cut into small pieces, lyophilised and co ...200717644408
increased levels of hpv16 e6*i transcripts in high-grade cervical cytology and histology (cin ii+) detected by rapid real-time rt-pcr cervical dysplasia may regress to normal cytology or progress to cervical carcinoma, it would be valuable to have a diagnostic tool to help decide whether therapeutic conization should be performed.200717662070
[efficacy of absorbance ratio of elisa antibodies [corrected] for hepatitis c virus of 3th generation in the prediction of viremia evaluated by pcr].in order to decrease the burden of suffering and the costs derived from confirmatory molecular assays, a better strategy is badly needed to decrease the rate of false positive results of the enzyme-linked immunoassay (elisa) for detection of hepatitis c virus (hcv) antibodies (anti).200717685198
comparative expression profiling of nicotiana benthamiana leaves systemically infected with three fruit tree viruses.plant viruses cause a wide array of disease symptoms and cytopathic effects. although some of these changes are virus specific, many appear to be common even among diverse viruses. currently, little is known about the underlying molecular determinants. to identify gene expression changes that are concomitant with virus symptoms, we performed comparative expression profiling experiments on nicotiana benthamiana leaves infected with one of three different fruit tree viruses that produce distinct s ...200717722703
effectiveness of polysaccharide pneumococcal vaccine in hiv-infected patients: a case-control study.polysaccharide pneumococcal vaccine (ppv) is recommended among human immunodeficiency virus (hiv)-infected patients, although its effect in reducing the incidence of pneumonia or invasive pneumococcal disease is not well established. our objective was to determine the effectiveness of 23-valent ppv in hiv-infected adults and the risk factors for pneumococcal pneumonia or invasive pneumococcal disease.200717806042
the emergence of porcine circovirus 2b genotype (pcv-2b) in swine in canada.since late 2004, the swine industry in the province of quebec has experienced a significant increase in death rate related to postweaning multisystemic wasting syndrome (pmws). to explain this phenomenon, 2 hypotheses were formulated: 1) the presence of a 2nd pathogen could be exacerbating the porcine circovirus 2 (pcv-2) infection, or 2) a new and more virulent pcv-2 strain could be infecting swine. in 2005, 13 pmws cases were submitted to the quebec provincial diagnostic laboratory and pcv-2 w ...200717824323
induction of immune responses in mice after intragastric administration of lactobacillus casei producing porcine parvovirus vp2 protein.lactobacillus casei atcc 393 was selected as an antigen delivery vehicle for mucosal immunization against porcine parvovirus (ppv) infection. a 64-kda fragment of ppv major protective antigen vp2 protein was used as the parvovirus antigen model. a recombinant lactobacillus expressing vp2 protein was constructed with plasmid ppg611.1, where expression and localization of the vp2 protein from recombinant lc393-rppv-vp2 was detected via sodium dodecyl sulfate-polyacrylamide gel electrophoresis, wes ...200717827311
role of myzus persicae (hemiptera: aphididae) and its secondary hosts in plum pox virus propagation.plum pox virus (family potyviridae, genus potyvirus, ppv) is one of the most important viral pathogens of plants in the genus prunus, particularly prunus persica l. the role of the myzus persicae (sulzer) (hemiptera: aphididae) as a vector of ppv-m, and its role in spreading ppv-m, was investigated. ppv-m-infected peach trees were used as inoculum sources, and transmission to 15 herbaceous species commonly present in and around peach orchards was evaluated. the presence of ppv-m in secondary hos ...200717849850
adaptation of plum pox virus to a herbaceous host (pisum sativum) following serial passages.plum pox virus (ppv) populations from peaches are able to adapt consistently to herbaceous hosts, characterized by a reduction in time to symptom development, increases in inoculation efficiency and increased titres. ppv adaptation was studied by using pea (pisum sativum) as an alternative host. two isolates of ppv from peaches were inoculated and passaged in peas ten times using either aphid or mechanical inoculation, generating four independent passage lines. mechanical-transmission efficiency ...200717872538
a chimeric plum pox virus shows reduced spread and cannot compete with its parental wild-type viruses in a mixed infection.the effect of a recombination event in the genomic 3' end on the biological properties and competitiveness of plum pox virus (ppv) was investigated. therefore, a fragment spanning the coat protein (cp) coding region and a part of the 3' non-translated region of a non-aphid-transmissible strain of ppv (ppv-nat) was replaced by the corresponding region of a ppv sour cherry isolate (ppv-soc). the resulting chimera (ppv-nat/soc) caused severe symptoms in nicotiana benthamiana, resembling those of pp ...200717872539
analysis of the complete genome of peach chlorotic mottle virus: identification of non-aug start codons, in vitro coat protein expression, and elucidation of serological cross-reactions.the entire genome of peach chlorotic mottle virus (pcmv), originally identified as prunus persica cv. agua virus (4n6), was sequenced and analysed. pcmv cross-reacts with antisera to diverse viruses, such as plum pox virus (ppv), genus potyvirus, family potyviridae; and apple stem pitting virus (aspv), genus foveavirus, family flexiviridae. the pcmv genome consists of 9005 nucleotides (nts), excluding a poly(a) tail at the 3' end of the genome. five open reading frames (orfs) were identified wit ...200717891331
electrophoretic mobility of the capsid protein of the plum pox virus strain ppv-rec indicates its partial phosphorylation.a double-band sds-page profile was found reproducible for capsid protein (cp) of plum pox virus (ppv) isolates belonging to the strain ppv-rec. the double-band was also present in the virus population multiplied in various plants. a single-lesion passage in a hypersensitive host chenopodium foetidum showed that its presence was not a result of a mixed infection. we found that the two electrophoretic forms of cp shared identical n-terminus. therefore, they did not originate from an alternative pr ...200717900221
gamma-glutamyltransferase and rapid virological response as predictors of successful treatment with experimental or standard peginterferon-alpha-2b in chronic hepatitis c non-responders.high-dose peginterferon-alpha (pegifn-alpha) induction and prolongation of therapy may be an option to improve sustained virological response (svr) rates among hepatitis c virus (hcv) non-responders, although a higher and a longer dosing of pegifn-alpha may intensify side effects.200717919233
oligonucleotide microarray-based detection and genotyping of plum pox virus.plum pox virus (ppv) is the most damaging viral pathogen of stone fruits. the detection and identification of its strains are therefore of critical importance to plant quarantine and certification programs. existing methods to screen strains of ppv suffer from significant limitations such as the simultaneous detection and genotyping of several strains of ppv in samples infected with different isolates of the virus. a genomic strategy for ppv screening based on the viral nucleotide sequence was d ...200817920703
demonstration of an association between detection of igg antibody reactivity towards the c-terminal region of the pres1 protein of hepatitis b virus and the capacity to respond to interferon therapy in chronic hepatitis b.the treatment of hepatitis b virus (hbv) remains complex, with somewhat unpredictable responses. the aim of this study was to determine the predictive value of the pretreatment presence of circulatory antibodies towards a synthetic peptide mimicking the amino acids 94-117 of the pres1 protein of hbv and the capacity to respond to alpha-inteferon (ifn-alpha) treatment.200817931371
[co-expression of csfv t cell epitope e290 peptide and ppv vp2 protein in lactobacillus casei and determination of specific antibodies in immunized mice].lactobacillus casei strain 393 was selected as an antigen delivery vehicle for the development of oral vaccine to express recombinant classical swine fever virus (csfv) t cell epitope e290 peptide and porcine parvovirus (ppv) vp2 protein. the recombinant genes encoding csfv t cell epitope e290 peptide and ppv vp2 protein, respectively, were cloned into the secretion expression vector ppg, and then the ppg-vp2-e290 was electrotransformed into l. casei 393 giving rise to recombinant strain ppg-vp2 ...200717944369
development of a novel hot-start multiplex pcr for simultaneous detection of classical swine fever virus, african swine fever virus, porcine circovirus type 2, porcine reproductive and respiratory syndrome virus and porcine parvovirus.a novel hot-start multiplex pcr (mpcr) assay was developed and subsequently evaluated for its effectiveness in simultaneously detecting multiple viral infections of swine. specific primers for each of five virus genomes, namely classical swine fever virus (csfv), african swine fever virus (asfv), porcine circovirus type 2 (pcv2), porcine reproductive and respiratory syndrome virus (prrsv) and porcine parvovirus (ppv) were used. combined nucleic acid purification was carried out using a commercia ...200817975735
no recombination detected in artificial potyvirus mixed infections and between potyvirus derived transgenes and heterologous challenging potyviruses.risk-assessment studies of virus-resistant transgenic plants (vrtps) focussing on recombination of a plant virus with a transgenic sequence of a different virus should include a comparison of recombination frequencies between viruses in double-infected non-transgenic plants with those observed in singly infected transgenic plants to estimate recombination incidence in vrtps. in this study, the occurrence of recombination events was investigated in non-transgenic plants double-infected with two d ...200718001687
specific and efficient cleavage of fusion proteins by recombinant plum pox virus nia proteases are the most popular kind of enzymes for removing the fusion tags from fused target proteins. nuclear inclusion protein a (nia) proteases obtained from the family potyviridae have become promising due to their high activities and stringencies of sequences recognition. nia proteases from tobacco etch virus (tev) and tomato vein mottling virus (tvmv) have been shown to process recombinant proteins successfully in vitro. in this report, recombinant ppv (plum pox virus) nia p ...200818024078
construction of recombinant lactobacillus casei efficiently surface displayed and secreted porcine parvovirus vp2 protein and comparison of the immune responses induced by oral immunization.lactobacillus casei atcc 393 was selected as a bacterial carrier for the development of mucosal vaccine against porcine parvovirus (ppv) infection. the ppv major structural polypeptide vp2 was used as the model parvovirus antigen. two inducible expression systems, namely ppg611.1 of the cell-surface expression system and ppg612.1 of the secretion expression system based on the xylose operon promoter were used to express the vp2 protein. the immunogenicity of recombinant strains producing vp2 pro ...200818034821
identification of plum pox virus pathogenicity determinants in herbaceous and woody hosts.plum pox virus (ppv) is a member of the genus potyvirus that is able to infect a large variety of plant species, including trees of the genus prunus, its natural host. when some ppv isolates are propagated for an extended time in herbaceous plants, their ability to infect trees is reduced. the molecular basis of this change in host infectivity is poorly understood. we report the construction of hybrid viruses from cdna clones of two d-strain isolates of ppv, ppv-d and ppv-r, which differ in thei ...200818052879
identification of liver fibrosis in hiv/hcv-coinfected patients using a simple predictive model based on routine laboratory data.we constructed noninvasive models to predict significant fibrosis (f > or = 2) and advanced fibrosis (f > or = 3) among human immunodeficiency virus (hiv)/hepatitis c virus (hcv)-coinfected patients, naïve for anti-hcv treatment. a total of 296 patients with liver biopsy were randomly assigned to an estimation group (eg = 226; 70%) and a validation group (vg = 70; 30%). we developed the hospital gregorio marañón (hgm)-1 index, based on platelet count, aspartate aminotransferase (ast) and glucose ...200718070289
role of the 25-26 nt sirna in the resistance of transgenic prunus domestica graft inoculated with plum pox virus.the reaction of a genetically engineered plum clone (c5) resistant to plum pox virus (ppv) by graft inoculation with the virus was evaluated. the resistance in this clone has been demonstrated to be mediated through post-transcriptional gene silencing (ptgs). a single c5 plant out of 30 plants inoculated with ppv m strain by double chip-budding showed mild diffuse mosaic 'sharka' symptom at the bottom section of the scion. the upper leaves of this ppv-infected c5 plant remained symptomless and t ...200818071892
undetectable hepatitis c virus rna at week 4 as predictor of sustained virological response in hiv patients with chronic hepatitis c.low baseline serum hepatitis c virus (hcv)-rna and clearance of viraemia at week 4 with pegylated interferon (pegifn) plus ribavirin therapy predict sustained virological response (svr) and enable a shorter duration of therapy in patients with chronic hepatitis c. it is unclear whether this applies to hiv/hcv-co-infected patients.200818090387
development of latex turbidimetric immunoassay for rapid and sensitive detection of influenza virus.for the rapid and sensitive detection of influenza a and b viruses, a latex turbidimetric immunoassay (ltia) was developed using latex reagents prepared by the sensitization of anti-influenza a or b monoclonal antibodies on latex particles. we measured the immunoreactivity of these latex reagents to influenza a and b viral antigens. the sensitivity and specificity of ltia and reverse transcription-polymerase chain reaction (rt-pcr) for the detection of these viruses in clinical specimens (96 nas ...200718154440
recurrent hepatitis c after liver transplantation: on-treatment prediction of response to peginterferon/ribavirin therapy.sustained virologic response (svr) in the treatment of recurrent hepatitis c virus (hcv) infection after liver transplantation (lt) remains suboptimal. we evaluated efficacy of pegylated interferon alfa (peg) and ribavirin (rbv) (peg/rbv) combination therapy in lt recipients with recurrent hcv and predictive values of rapid virological response (rvr) and early virologic response (evr). between january 2001 and october 2005, lt recipients with recurrent hcv were intended to be treated for 48 week ...200818161839
performance of noninvasive markers for liver fibrosis is reduced in chronic hepatitis c with normal chronic hepatitis c, biopsy is the gold standard for assessment of liver fibrosis. few studies investigated noninvasive markers of liver fibrosis in hepatitis c virus (hcv) patients with normal alanine aminotrasferase (nalt). eighty hcv patients with nalt and 164 hcv patients with elevated alanine aminotrasferase (ealt) who underwent a diagnostic liver biopsy were evaluated for ast-to-platelet ratio, forns' index, ast-to-alt ratio (aar), fibrotest and the recently proposed fibroindex, using l ...200818179453
identification of quantitative trait loci controlling symptom development during viral infection in arabidopsis compatible interactions between plants and viruses that result in systemic infection, symptom development is a major phenotypic trait. however, host determinants governing this trait are mostly unknown, and the mechanisms underlying it are still poorly understood. in a previous study on the arabidopsis thaliana-plum pox virus (ppv) pathosystem, we showed a large degree of variation in symptom development among susceptible accessions. in particular, cvi-1 (cape verde islands) accumulates viral ...200818184064
role of rapid virological response in prediction of sustained virological response to peg-ifn plus ribavirin in hcv / hiv co-infected individuals.the objective of the study was to evaluate the role of rapid virological response (rvr) in predicting sustained virological response (svr) rates to hepatitis c virus (hcv) therapy. 65 hiv / hcv co-infected patients commenced hcv treatment per protocol. hiv / hcv patients with a mean cd4 count of 502 were treated for 24-48 weeks depending on genotype. virological response was assessed at weeks 4 (rvr), 12 [early virological response (evr)], 24, at end of treatment (eotr) and 24 weeks post-complet ...200818221297
tuberculin skin test results in hiv-infected patients in india: implications for latent tuberculosis evaluate the utility of the tuberculin skin test (tst) in detecting latent and active tuberculosis (tb) among human immunodeficiency virus (hiv) infected patients in south india.200818230249
indirect estimation of porcine parvovirus maternal immunity decay in free-living wild boar (sus scrofa) piglets by capture-recapture data.linear mixed regression and logspline density estimation were performed to estimate the survival curve and half life of passively acquired antibodies against porcine parvovirus (ppv) in 44 wild boar piglets captured in the northern apennines, italy. one piglet had no detectable maternal antibodies at 2.5 months post partum and no antibodies were detected in any of the remaining piglets by 4 months of age. fitted survival curves indicated that maternal antibodies were undetectable from 2.5 to 6 m ...200918295517
ebv pcr in the diagnosis and monitoring of posttransplant lymphoproliferative disorder: results of a two-arm prospective trial.while ebv pcr is used in the management of ptld, the optimal primer set, relative importance of intracellular versus free plasma ebv, and the baseline profile in an organ transplant population remains unclear. we performed a prospective 2-arm trial utilizing an ebv pcr panel measuring lmp-1, eber-1 and ebna-1 in both free plasma as well as intracellular whole blood. control arm a consisted of 31 lung transplant patients and arm b consisted of 35 transplant patients being evaluated for possible p ...200818312608
optimized cutoffs improve performance of the aspartate aminotransferase to platelet ratio index for predicting significant liver fibrosis in human immunodeficiency virus/hepatitis c virus assess the diagnostic value of modified cutoffs for aspartate aminotransferase to platelet ratio index (apri) to predict significant liver fibrosis in human immunodeficiency virus (hiv)/hepatitis c virus (hcv) patients.200818339075
phylogenetic analysis of porcine circovirus type 2 (pcv2) pre- and post-epizootic postweaning multisystemic wasting syndrome (pmws).the porcine circovirus type 2 (pcv2) genome encodes three major open reading frames (orfs) encoding the replicase proteins (orf1), the viral capsid protein (orf2), and a protein with suggested apoptotic activity (orf3). previous phylogenetic analyses of complete genome sequences of pcv2 from genbank have demonstrated 95-100% intra-group nucleotide sequence identity. however, although these isolates were readily grouped into clusters and clades, there was no correlation between the occurrence of ...200818343985
optimizing screening for acute human immunodeficiency virus infection with pooled nucleic acid amplification tests.recent studies have shown the public health importance of identifying individuals with acute human immunodeficiency virus infection (ahi); however, the cost of nucleic acid amplification testing (naat) makes individual testing of at-risk individuals prohibitively expensive in many settings. pooled naat (or group testing) can improve efficiency and test performance of testing for ahi, but optimizing the pooling algorithm can be difficult. we developed simple, flexible biostatistical models of spe ...200818353930
rapid detection of porcine circovirus type 2 by loop-mediated isothermal amplification.a method of loop-mediated isothermal amplification (lamp) was employed to develop a rapid and simple detection system for porcine circovirus type 2 (pcv2). the amplification could be finished in 60 min under isothermal condition at 64 degrees c by employing a set of four primers targeting the cap gene of pcv2. the lamp assay showed higher sensitivity than the conventional pcr, with a detection limit of five copies per tube of purified pcv2 genomic dna. no cross-reactivity was observed from the s ...200818355932
phylogenetic analysis of members of the phycodnaviridae virus family, using amplified fragments of the major capsid protein gene.algal viruses are considered ecologically important by affecting host population dynamics and nutrient flow in aquatic food webs. members of the family phycodnaviridae are also interesting due to their extraordinary genome size. few algal viruses in the phycodnaviridae family have been sequenced, and those that have been have few genes in common and low gene homology. it has hence been difficult to design general pcr primers that allow further studies of their ecology and diversity. in this stud ...200818359826
results at recruitment from a randomized controlled trial comparing human papillomavirus testing alone with conventional cytology as the primary cervical cancer screening the first recruitment phase of a randomized trial of cervical cancer screening methods (new technologies for cervical cancer screening [ntcc] study), we compared screening with conventional cytology with screening by human papillomavirus (hpv) testing in combination with liquid-based cytology. hpv-positive women were directly referred to colposcopy if aged 35 or older; if younger, they were retested after 1 year.200818364502
kinetics of inactivating human parvovirus b19 and porcine parvovirus by dry-heat treatment. 200818366462
development of recombinant porcine parvovirus-like particles as an antigen carrier formed by the hybrid vp2 protein carrying immunoreactive epitope of porcine circovirus type 2.virus-like particles (vlps) are non-replicative vectors for delivery of heterologous epitopes and induction of immune responses. in this study, a self-assembled porcine parvovirus (ppv) vp2 capsid protein [ppv:vlp-(pcv2)] carrying immunoreactive epitopes, residues 165-200 from the porcine circovirus 2 (pcv2) virus nucleoprotein was constructed. immunogenicity study was carried out with hybrid vlps derived from hek-293 cells infected with recombinant adenovirus vectors. to our knowledge, this stu ...200818378364
des-gamma-carboxyprothrombin, alpha-fetoprotein and afp-l3 in patients with chronic hepatitis, cirrhosis and hepatocellular carcinoma.hepatocellular carcinoma (hcc) is a common complication in patients with chronic viral hepatitis. detection of hcc at an early stage is critical for a favorable clinical outcome. the study aim was to: (i) compare the levels of des-gamma-carboxyprothrombin (dcp), alpha-fetoprotein (afp) and afp-l3 in hcc patients and in chronic viral hepatitis patients without hcc; (ii) define the level of each tumor marker with the best sensitivity and specificity for hcc diagnosis; and (iii) to correlate the le ...200818422961
epstein-barr virus (ebv)-encoded rna: automated in-situ hybridization (ish) compared with manual ish and immunohistochemistry for detection of ebv in pediatric lymphoproliferative disorders.detection of epstein-barr virus (ebv) may be achieved by various methods, including ebv-encoded rna (eber) in-situ hybridization (ish) and immunohistochemistry (ihc) for latent membrane protein (lmp-1). we compared novel automated ish and ihc techniques in pediatric lymphoproliferative disorders with results obtained by manual ish. thirty-seven pediatric cases previously studied by manual eber ish (including 18 eber-positive, 15 eber-negative, and 4 eber-equivocal cases) were used for the study. ...200918442302
[short communication: the sensitivity of measles diagnosis by physicians and families during an intraepidemic period in edirne: implications for measles surveillance].measles is still a leading cause of death among young children, despite the availability of a safe and effective vaccine for the past 40 years. euro region of world health organisation including turkey has targeted elimination of measles by the year 2010. it is concluded that there must be a sensitive surveillance system to investigate all suspicious measles cases, and diagnosis should be based on both standardized case definition and laboratory confirmation. standardized case definition based n ...200818444573
impact of pneumococcal vaccination on the incidence of pneumonia by hiv infection status among patients enrolled in the veterans aging cohort 5-site study.human immunodeficiency virus (hiv)-infected persons have a high incidence of pneumonia and pneumococcal disease. benefits of vaccination with the 23-valent pneumococcal polysaccharide vaccine (ppv) among these patients continue to be debated.200818444830
the predictive value of cerebrospinal fluid epstein-barr viral load as a marker of primary central nervous system lymphoma in hiv-infected persons.the presence of epstein-barr virus (ebv) dna in cerebrospinal fluid (csf) is used as a marker of hiv-associated primary central nervous system lymphoma (pcnsl). in our setting, ebv dna is frequently detected in the csf of hiv-infected patients with miscellaneous neurological diseases and thus its presence is a poor predictor of pcnsl.200818455472
evaluation of commercial ebv recombline assay for diagnosis of nasopharyngeal recent years a number of epstein-barr virus (ebv) proteins were defined as being immunodominant for either igm, igg or iga immune responses, yielding promising markers for diagnostic serology. specific reactivity patterns to these proteins have been described for infectious mononucleosis (im), nasopharyngeal carcinoma (npc), various types of lymphoma, and healthy ebv carriers.200818455473
[meta-analysis for the pooled sensitivity and specificity of hepatitis b surface antigen rapid tests].although hepatitis b surface antigen (hbsag) rapid test based on immunochromatographic assay (ica) is now widely used, the test has not been evaluated sufficiently enough to validate its performance. thus, it is important to summarize the clinical performance of the test kits. in this study, we performed meta-analysis for the performance of the hbsag rapid tests.200818458514
evaluation of the performance of four rapid tests for detection of hepatitis b surface antigen in antananarivo, madagascar.four rapid immunochromatographic assays--determine hbsag, virucheck hbsag, cypress hbsag dipstick and hexagon hbsag--for human hepatitis b surface antigen (hbsag) detection in human serum were evaluated. a collection of reference serum samples (91 hbsag positive and 109 hbsag negative) stored at -80 degrees c was used. sensitivity and positive predictive value (ppv) exceeded 95%, and specificity and negative predictive value (npv) exceeded 96% for all tests. the determine hbsag test performed be ...200818462816
identification of trimeric peptides that bind porcine parvovirus from mixtures containing human blood plasma.virus contamination in human therapeutics is of growing concern as more therapeutic products from animal or human sources come into the market. all biopharmaceutical processes are required to have at least two distinct viral clearance steps to remove viruses. most of these steps work well for enveloped viruses and large viruses, whether enveloped or not. that leaves a class of small non-enveloped viruses, like parvoviruses and hepatitis a, which are not easily removed by these typical steps. in ...200818470993
rapid immunoassay for the detection of genital herpes infection.we evaluated a new affinity membrane strip test for the diagnosis of herpetic genital infections. test strip results, which are available by immunoassay in 30 min without the need for special equipment, were compared with the results of viral culture.199418475363
performance characteristics of a new rapid immunochromatographic test for the detection of antibodies to human immunodeficiency virus (hiv) types 1 and 2.diagnostic kits for the detection of human immunodeficiency virus (hiv) antibodies have reached an unprecedented number. but choice of an ideal, cost-effective, and rapid test for hiv infection is of immense value for use in developing countries like india, where resources are limited. in this study we have evaluated the performance characteristics of the rapid immunochromatographic hiv test kit first response hiv 1-2.o. first, the laboratory archived 450 characterized plasma/serum specimens, wh ...200818484656
monitoring of bk virus replication in the first year following renal transplantation.bk virus-associated nephropathy (bkvan) is one of the most common viral diseases affecting renal allografts. screening for viral replication may allow for earlier intervention with reduced allograft loss. a plasma viral load >10(4) copies/ml of bkv dna is recommended for a presumed diagnosis of bkvan.200818503098
a laboratory study of survival of selected microorganisms after heat treatment of biowaste used in biogas plants.the aim of the study was to assess the effect of pasteurisation, as set by the european regulation ec 1774/2002, on selected pathogens and indicator organisms. unpasteurised substrate (biowaste), including animal by-products from a full-scale biogas plant was heat treated under laboratory conditions at 70 degrees c and 55 degrees c for 30 min and 60 min. heat treatment at 55 degrees c for 60 min was not sufficient to achieve a hygienically acceptable product. heat treatment at 70 degrees c for 3 ...200818513960
alteration in the chloroplastic metabolism leads to ros accumulation in pea plants in response to plum pox this work, a recombinant plum pox virus (ppv, sharka) encoding green fluorescent protein is used to study its effect on antioxidant enzymes and protein expression at the subcellular level in pea plants (cv. alaska). ppv had produced chlorotic spots as well as necrotic spots in the oldest leaves at 13-15 d post-inoculation. at 15 d post-inoculation, ppv was present in the chlorotic and necrotic areas, as shown by the fluorescence signal produced by the presence of the green fluorescent protein ...200818535298
molecular basis for porcine parvovirus detection in dead fetuses.reproductive failures are still common grounds for complaint by commercial swine producers. porcine parvovirus (ppv) is associated with different clinical reproductive signs. the aim of the present study was to investigate ppv fetal infection at swine farms having ongoing reproductive performance problems. the presence of virus in fetal tissues was determined by nested-polymerase chain reaction assay directed to the conserved ns1 gene of ppv in aborted fetuses, mummies and stillborns. fetuses sh ...200818561384
plum pox virus variability detected by the advanced analytical methods.plum pox virus (ppv) infects stone-fruit trees with important economical impact mainly in europe and mediterranean region. the data about ppv intra-species variability accumulated markedly in the last two decades. six ppv strains have been recognized using different approaches including serology, protein analysis, specific amplification, and genome sequencing. reliable and sensitive diagnostics is the most important requirement for application of early control and safety measures. therefore, man ...200818564894
altered gene expression changes in arabidopsis leaf tissues and protoplasts in response to plum pox virus infection.virus infection induces the activation and suppression of global gene expression in the host. profiling gene expression changes in the host may provide insights into the molecular mechanisms that underlie host physiological and phenotypic responses to virus infection. in this study, the arabidopsis affymetrix ath1 array was used to assess global gene expression changes in arabidopsis thaliana plants infected with plum pox virus (ppv). to identify early genes in response to ppv infection, an arab ...200818613973
dry-heat treatment process for enhancing viral safety of an antihemophilic factor viii concentrate prepared from human plasma.viral safety is a prerequisite for manufacturing clinical antihemophilic factor viii concentrates from human plasma. with particular regard to the hepatitis a virus (hav), a terminal dry-heat treatment (100 degrees for 30 min) process, following lyophilization, was developed to improve the virus safety of a solvent/detergent-treated antihemophilic factor viii concentrate. the loss of factor viii activity during dry-heat treatment was of about 5%. no substantial changes were observed in the physi ...200818633304
improvement of virus safety of an antihemophilc factor ix by virus filtration process.viral safety is an important prerequisite for clinical preparations of plasma-derived pharmaceuticals. one potential way to increase the safety of therapeutic biological products is the use of a virus-retentive filter. in order to increase the viral safety of human antihemophilic factor ix, particularly in regard to non-enveloped viruses, virus removal process using a polyvinylidene fluoride membrane filter (viresolve nfp) has been optimized. the most critical factor affecting the filtration eff ...200818667862
identification of restriction endonuclease with potential ability to cleave the hsv-2 genome: inherent potential for biosynthetic versus live recombinant microbicides.herpes simplex virus types 1 and 2 are enveloped viruses with a linear dsdna genome of approximately 120-200 kb. genital infection with hsv-2 has been denoted as a major risk factor for acquisition and transmission of hiv-1. developing biomedical strategies for hsv-2 prevention is thus a central strategy in reducing global hiv-1 prevalence. this paper details the protocol for the isolation of restriction endunucleases (reases) with potent activity against the hsv-2 genome and models two biomedic ...200818687114
hepatitis vaccination in patients with hepatitis c: practice and validation of codes at a large veterans administration medical centre.hepatitis vaccination is recommended in patients with chronic liver disease.200818691350
host-specific effect of p1 exchange between two potyviruses.the potyviruses plum pox virus (ppv) and tobacco vein mottling virus (tvmv) have distinct host ranges and induce different symptoms in their common herbaceous hosts. to test the relevance of the p1 protein in host compatibility and pathogenicity, hybrid viruses were constructed in which the p1 coding sequence of ppv was completely or partially replaced by the corresponding sequences from tvmv. infections induced by these chimeric viruses revealed that the tvmv p1 and a ppv/tvmv hybrid p1 protein ...200818705848
use of luminex xmap-derived bio-plex bead-based suspension array for specific detection of ppv w and characterization of epitopes on the coat protein of the virus.a panel of monoclonal antibodies (mabs) directed against the n-terminus region of the coat protein (cp) of strain ppv w (isolate 3174) was generated by immunizing mice with recombinant peptides. the best performing mabs were identified as 2c3 and 10g7. mab 2c3 was selected for comparison of a standard tas-elisa protocol with a luminex xmap technology-derived bead-based suspension array system described as a triple antibody sandwich-microsphere immunoassay (tas-mia). tas-mia was as sensitive as t ...200818722476
consensus recommendations on immunization, 2008.justification: there is a need to formulate recommendations regarding use of new vaccines which have become recently available/will soon be available and to review/revise recommendations about existing vaccines in light of recent information.200818723905
performance of indirect immunofluorescence assay, immunochromatography assay and reverse transcription-polymerase chain reaction for detecting human respiratory syncytial virus in nasopharyngeal aspirate samples.comparison of the use of indirect immunofluorescence assay (ifa), immunochromatography assay (ica-bd) and reverse transcription-polymerase chain reaction (rt-pcr) for detecting human respiratory syncytial virus (hrsv) in 306 nasopharyngeal aspirates samples (npa) was performed in order to assess their analytical performance. by comparing the results obtained using ica-bd with those using ifa, we found relative indices of 85.0% for sensitivity and 91.2% for specificity, and the positive (ppv) and ...200818797759
plum pox in north america: identification of aphid vectors and a potential role for fruit in virus spread.abstract thirteen aphid species were tested for their ability to transmit pennsylvania isolates of plum pox virus (ppv) collected in columbia (penn-3), franklin (penn-4), and york (penn-7) counties, pa. four species, aphis fabae, a. spiraecola, brachycaudus persicae, and myzus persicae, consistently transmitted ppv in preliminary transmission tests. two species, metopolophium dirhodum and rhopalosiphum padi, were occasional inefficient vectors. toxoptera citricida, from florida, also was an effe ...200418943108
spatial pattern analysis of sharka disease (plum pox virus strain m) in peach orchards of southern france.abstract the spatial pattern of sharka disease, caused by plum pox virus (ppv) strain m, was investigated in 18 peach plots located in two areas of southern france. ppv infections were monitored visually for each individual tree during one to three consecutive years. point pattern and correlation-type approaches were undertaken using the binary data directly or after parsing them in contiguous quadrats of 4, 9, and 16 trees. ordinary runs generally revealed a low but variable proportion of rows ...200318943618
factors affecting the spread of plum pox virus strain m in peach orchards subjected to roguing in france.abstract we evaluated the impact of roguing on the spread and persistence of the aggressive plum pox virus strain m (ppv-m) in 19 peach orchard blocks in southern france. during a 7- to 10-year period, orchards were visually inspected for ppv symptoms, and symptomatic trees were removed every year. disease incidence was low in all orchards at disease discovery and was <1% in 16 of the 19 orchard blocks. the spread of sharka disease was limited in all 19 blocks, with an annual disease incidence b ...200418943711
identification of plum pox virus determinants implicated in specific interactions with different prunus spp.abstract the characterization of pathogenic properties of two infectious clones of plum pox virus (ppv) isolates, pgppv (d group) and pgppvps (m group), was investigated in their woody hosts (seedlings of prunus spp.). the two clones differed in their ability to infect plum and peach cultivars, from no infection to local and systemic infection. the phenotype determinants were located with a set of chimeric viruses from the two clones. in plum, determinants of systemic infection were located in a ...200118944389
a single amino acid mutation in the plum pox virus helper component-proteinase gene abolishes both synergistic and rna silencing suppression activities.abstract the effects on symptom expression of single amino acid mutations in the central region of the plum pox virus (ppv) helper component-proteinase (hc-pro) gene were analyzed in nicotiana benthamiana using potato virus x (pvx) recombinant viruses. pvx recombinant virus expressing the wild-type variant of ppv hc-pro induced the expected enhancement of pvx pathogenicity, manifested as necrosis and plant death. recombinant virus expressing a variant of ppv hc-pro containing a single point muta ...200518944411
interference between d and m types of plum pox virus in japanese plum assessed by specific monoclonal antibodies and quantitative real-time reverse transcription-polymerase chain reaction.abstract the dynamics of virus interference between two isolates of plum pox virus (ppv) belonging to the main ppv types, d and m, were analyzed in japanese plum (prunus salicina) by challenge inoculations. to assess the consequences of a ppv-m infection on plum already infected with ppv-d, and vice versa (predominance of one of the strains, recombination, synergism, symptoms aggravation, and so on), 30 japanese plum trees were graft inoculated with ppv-d or ppv-m isolates in quarantine conditio ...200618944448
comparison of monoclonal antibodies and polymerase chain reaction assays for the typing of isolates belonging to the d and m serotypes of plum pox potyvirus.abstract plum pox potyvirus (ppv) isolates may be divided into four groups separated by serological, molecular, and epidemiological differences. monoclonal antibodies specific for the two major groups of isolates, represented by the d and m serotypes of the virus, have been obtained. polymerase chain reaction (pcr)-based assays allowing the direct detection and differentiation of ppv isolates have also been developed. we now report on a large-scale comparison of these two typing approaches. the ...199818944965
an evaluation of saliva as an alternative to plasma for the detection of hepatitis c virus antibodies.seroepidemiological studies on the prevalence of hepatitis c virus (hcv) in india have been hampered by reluctance of subjects to provide blood samples for testing. we evaluated the use of saliva as an alternate specimen to blood for the detection of antibodies to hcv.200818974484
reproduction of postweaning multisystemic wasting syndrome in pigs by prenatal porcine circovirus 2 infection and postnatal porcine parvovirus infection or immunostimulation.postweaning multisystemic wasting syndrome (pmws) was reproduced in prenatally porcine circovirus 2 (pcv2)-infected pigs by either postnatal infection with porcine parvovirus (ppv) or by immunostimulation. twenty-four randomly selected piglets from 3 sows, which had been experimentally infected during gestation with pcv2, were randomly divided into 3 groups; group 1 (prenatal pcv2 infection, with postnatal ppv infection), group 2 (prenatal pcv2 infection, with postnatal keyhole limpet hemocyanin ...200818984787
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