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properties of a virus causing mosaic and leaf curl disease of celosia argentea l. in nigeria.a sap transmissible virus, causing mosaic and leaf curl disease of celosia argentea, was isolated at vegetable farms in amuwo odofin, tejuoso, and abule ado, lagos, nigeria. the virus had a restricted host range confined to a few species of the amaranthaceae, chenopodiaceae and solanaceae families. it failed to infect several other species of the aizoaceae, brassicaceae, cucurbitaceae, fabaceae, lamiaceae, malvaceae, poaceae and tiliaceae families. the virus was transmitted in a non-persistent m ...19989842442
development of a pcr-based method coupled with a microplate colorimetric assay for the detection of porcine parvovirus and application to diagnosis in piglet tissues and human plasma.a new method for porcine parvovirus (ppv) diagnosis was developed. the method is based on polymerase chain reaction (pcr) amplification followed by hybridization and colorimetric detection of pcr products in microwell plates. a highly specific and sensitive amplification step was ensured by primers carefully selected in the vp2 structural gene and optimized pcr conditions. uracyl-dna-glycosylase (udg) in combination with dutp was used to avoid false-positive results, and 100 copies of internal c ...19989843658
preliminary report on the apparent breaking of resistance of a transgenic plum by chip bud inoculation of plum pox virus ppv-s.five transgenic clones of prunus domestica l. containing plum pox virus (ppv) coat protein (cp) gene and one non-transformed control clone were challenged with ppv-s in the field. symptoms developed on c2, c3, c4, c6 and b70146 but not c5 trees inoculated by chip budding (cbi) (2/2, 2/2, 1/1, 2/2 and 2/2, positive/inoculated) in the first summer after inoculation. however, in the second year, symptoms appeared on cbi c5 trees. the presence of the virus in the plants was confirmed by enzyme-linke ...199810073228
new results concerning the plum pox virus epidemiology and resistance of plum cultivars, hybrids and rootstocks.our recent results on the plum pox virus (ppv) epidemiology show that ppv spreads very rapidly in plum tree plantations in the contaminated areas. a clearing of the ppv-infected trees reduces significantly the spread of the virus but does not eliminate the disease. some plum tree cultivars, hybrids and rootstocks (scoldus, alina, cristi, bn 1/8fl, bn 2gr. etc) showing field resistance could not be infected with ppv by natural way. however, they could be infected with ppv by artificial inoculatio ...199810073229
nucleotide sequence of the putative replicase gene of the sour cherry strain of plum pox potyvirus.the complete nucleotide sequence of the nib coding region of the sour cherry strain of plum pox potyvirus (ppv-soc) has been determined. it consists of 1554 nucleotides and encodes a putative replicase protein of 518 amino acids. sequence identity scores between nib of ppv-soc and other isolates of ppv are significantly low (c. 78%). many of the nucleotide substitutions, however, are silent. ppv-soc differs from isolates of ppv-d, ppv-m and ppv-e1 amar at multiple amino acid positions that are c ...19989856106
use of modified plum pox virus coat protein genes developed to limit heteroencapsidation-associated risks in transgenic plants.aphid transmission of a non-aphid-transmissible strain of zucchini yellow mosaic virus (zymv-nat) occurs in transgenic plants expressing the plum pox potyvirus (ppv) coat protein (cp) gene. heteroencapsidation has been shown to be responsible for this modification in the epidemiological characteristics of the infecting virus. in order to prevent this biological risk, several modified ppv cp constructs were produced that were designed to interfere with heteroencapsidation itself or to block aphid ...19989634095
production of a monoclonal antibody specific to the el amar strain of plum pox virus.plum pox virus (ppv) isolates are grouped into three clusters differentiated by biological, serological, molecular and epidemiological characteristics: marcus (m), dideron (d) and cherry (c). the el amar (ea) isolate that does not fit any of the above groups is also known. monoclonal antibodies (mabs) that specifically recognize m, d, and c strains of ppv are already available. to complete the set of ppv strain-specific serological reagents, mabs against the ea isolate were raised by immunizing ...199810073230
some results of 50 years of research on the resistance to plum pox virus.over 300 references on the resistance of stone fruit species to plum pox virus (ppv) have been utilized for the summarization of relevant information in this review. methods of testing ppv resistance, procedures of evaluation and characterization of ppv resistance as well as breeding of ppv-resistant cultivars are briefly discussed. altogether 370 cultivars, hybrids and clones of plum, peach, apricot, nectarine and wild prunus species are tabulated together with the authors who have reported on ...199810073219
relationship between concentration of plum pox virus and content of pigments in virus-infected symptomatic apricot leaves.besides other factors, occurrence of plum pox virus (ppv)-caused spots and mosaic symptoms on leaves of stone fruits are known to influence important physiological functions including production of assimilates. apricot (prunus armeniaca l.) seedlings were used as a test material for typical manifestation of symptoms of the disease on the foliage. the relations of the abovementioned visual symptoms to the virus and pigments concentrations in leaves have so far not been known sufficiently. we dete ...199810073220
detection and serotyping of mediterranean plum pox virus isolates by means of strain-specific monoclonal antibodies.plum pox virus (ppv) is a major threat to the expanding mediterranean stone fruit industry. in order to control the plum pox disease it is of utmost importance to detect early ppv foci and to identify the ppv isolates involved. a survey was therefore carried out in albania, cyprus, egypt, greece, italy and turkey by a double-antibody sandwich enzyme-linked immunosorbent assay (das-elisa) with the following monoclonal antibodies (mabs): 5b (universal), 4dg5 (ppv-d-specific), al (ppv-m-specific), ...199810073231
detection of plum pox virus in apricot seeds.twelve different apricot selection trees from a germplasm collection naturally infected with plum pox virus (ppv) were chosen to investigate the role of seeds in the epidemiology of this dangerous pathogen. all the considered plants showed typical symptoms on leaves and fruits and were positive in enzyme-linked immunosorbent assay (elisa). the virus was characterized by immunocapture reverse transcription-polymerase chain reaction (ic-rt-pcr) followed by restriction fragment length polymorphism ...199810073234
relative concentration of plum pox virus in leaves and flowers of some prunus species and cultivars.the relative concentration of plum pox virus (ppv) in leaves and flowers of plum, damson, myrobalan, blackthorn, apricot and peach trees was determined by enzyme-linked immunosorbent assay (elisa) and expressed as the lowest dilution with positive reaction. significant differences in relative ppv concentration were found in leaves among individual prunus species naturally or artificially infected with the virus. the highest relative ppv concentration was found in blackthorns (7.81 x 10(-4)), com ...199810073235
restriction fragment length polymorphism differentiation of plum pox virus isolates.reverse transcription-polymerase chain reaction (rt-pcr) technique and restriction fragment length polymorphism (rflp) analysis were used to analyse six isolates of plum pox virus (ppv). whole coat protein (cp) gene was amplified in four isolates using the unipoty-polyt primer pari. ppv-d was identified by rflp analysis using sfui and drai enzymes in two of the isolates. two isolates of ppv-m strain yielded rt-pcr products which could not be digested by the two enzymes. other isolates were subje ...199810073236
characterization of phenotype resistance to plum pox of transgenic plums expressing plum pox virus capsid gene.resistance to plum pox virus (ppv) infection can be obtained in transgenic plants that express the virus capsid gene. an agrobacterium-mediated transformation was used to introduce the ppv capsid gene into prunus domestica plants. over 11 regenerated plants (clones) were observed for the development of the disease symptoms and analysed for the presence of ppv by enzyme-linked immunosorbent assay (elisa), western blot analysis, and reverse transcription-polymerase chain reaction (rt-pcr) through ...199810073237
agroclimatical analysis of plum pox virus spread in slovakia.in this report, the plum pox virus (ppv) spread from the point of view of agroclimatical conditions on the territory of slovakia is analysed. the worst condition for the ppv spread was found in a warm macroregion. the air temperature sum in this macroregion during vegetative period ranged from 2400 to 3100 degrees c. the worst condition for the ppv spread was found in dry subregions of the macroregion. these were defined by differences between potential evapotranspiration and rainfall sums which ...199810073238
detection of plum pox virus by enzyme-linked immunosorbent assay in some apricot and peach varieties and hybrids in romania.plum pox virus (ppv) is a potyvirus widely spread in many species of the prunus genus such as plum, apricot, peach, sweet cherry and others. this potyvirus causes great damage to stone fruit trees in romania and other european countries as hungary, italy, czech republic, france, spain, greece, turkey, and slovak republic. the research station for fruit tree growing baneasa in bucharest has realized many studies on the epidemiology and spread of ppv and also on the disease symptomatology and dete ...199810073239
new methods for inactivation of lipid-enveloped and non-enveloped viruses.two new methods are described for inactivating lipid-enveloped and non-enveloped viruses in plasma-derived products such as coagulation factors and intravenous immunoglobulin (igiv). iodine/sephadex delivers iodine to igiv solutions in a slow, controlled way and allows for inactivation of > or = 4 logs of porcine parvovirus (ppv), a hardy non-enveloped virus, under conditions which do not measurably damage the structural or functional properties of the igiv, and with essentially no iodination of ...19989873761
specific oligonucleotide primers for the direct detection of plum pox potyvirus-cherry subgroup.a specific polymerase chain reaction assay was developed for direct identification of the distinct subgroup of plum pox potyvirus (ppv) isolates from cherry trees (ppv-cherry, ppv-c) and its differentiation from other known subgroups of ppv. the specificity of the assay is based on using a pair of primers whose nucleotide sequences are located on the coat protein gene of ppv-sour cherry (soc) at regions of high nucleotide divergence between ppv-soc and other isolates of ppv. the technique will b ...19989562418
changing disease patterns in focal brain lesion-causing disorders in aids.to assess temporal trends of the different disorders causing focal brain lesions (fbl) in hiv-infected patients and to examine the reliability of the u.s. centers for disease control and prevention (cdc) criteria for presumptive diagnosis of toxoplasmic encephalitis (te) for the years 1991 to 1996.19989704942
detection of challenge virus in fetal tissues by nested pcr as a test of the potency of a porcine parvovirus vaccine.to estimate the potency of a porcine parvovirus (ppv) vaccine, three vaccinated and three non-vaccinated pregnant gilts were infected with ppv and the distribution of the virus was studied in the tissues of their 51 fetuses. virus detection was attempted using haemagglutination (ha) and immunofluorescence (if) assays, as well as by standard (single) and nested polymerase chain reactions (pcr). none of the detection methods yielded positive results when used to test for the presence of virus in s ...19989563172
a recombinant virus-like particle system derived from parvovirus as an efficient antigen carrier to elicit a polarized th1 immune response without adjuvant.hybrid virus-like particles (vlp) were prepared by self-assembly of the modified porcine parvovirus (ppv) vp2 capsid protein carrying a cd8+ or cd4+ t cell epitope. immunization of mice with a single dose of these hybrid pseudo-particles, without adjuvant, induced strong cytotoxic t lymphocyte and t helper (th) responses against the reporter epitope. the th response was characterized by a th1 phenotype. we also analyzed in vitro the uptake mechanism of these parvovirus-like particles and the pro ...19989565380
analysis of genomic rearrangement and subsequent gene deletion of the attenuated orf virus strain d1701.the orf virus (ov) strain d1701 belongs to the genetically heterogenous parapoxvirus (ppv) genus of the family poxviridae. the attenuated ov d1701 has been licensed as a live vaccine against contagious ecthyma in sheep. detailed knowledge on the genetic structure and organization of this ppv vaccine strain is an important prerequisite to reveal possible genetic mechanisms of ppv attenuation. the present study demonstrates a genomic map of the approximately 158 kbp dna of ov d1701 established by ...19989784065
determination of adequate moisture content for efficient dry-heat viral inactivation in lyophilized factor viii by loss on drying and by near infrared spectroscopy.a requirement for a minimal threshold level of moisture in order for efficient virus inactivation to occur during dry heat treatment of freeze-dried coagulation factor concentrates is described. techniques used to determine moisture content during heating were loss on drying and karl fischer. the loss on drying was suspected to have occasional errors as a result of sample preparation being influenced by interference from atmospheric moisture. therefore, a non-invasive, non-destructive method for ...19989811517
inactivation of viruses by beta-propiolactone in human cryo poor plasma and igg concentrates.virus inactivation by cold treatment with beta-propiolactone (bpl) was investigated in human cryo poor plasma and purified igg concentrates spiked with relevant human viruses or appropriate animal model viruses. the samples were treated with 0.1 or 0.25% bpl for 300 or 480 min, respectively. residual infectivity was determined by standard microtitration assays on tissue culture cells. the inactivation of all viruses tested was more effective in igg than in plasma. igg: r1=4-5.5 log10 for vesicul ...19989811521
prenatal diagnosis of congenital cytomegalovirus infection.we report here the results of a study on the prenatal diagnosis of congenital cytomegalovirus (cmv) infection. the study was carried out by both pcr and virus isolation from amniotic fluid (af) for 82 pregnant women at risk of transmitting cmv for the detection of (i) seroconversion to cmv immunoglobulin g (igg) positivity during the first trimester of pregnancy, (ii) symptomatic cmv infection in the mother during the first trimester of pregnancy or intrauterine growth retardation detected by ul ...19989817869
mapping the antigenic structure of porcine parvovirus at the level of peptides.the antigenic structure of the capsid proteins of porcine parvovirus (ppv) was investigated. a total of nine linear epitopes were identified by pepscan using porcine or rabbit anti-ppv antisera. no sites were identified with a panel of neutralising monoclonal antibodies (mabs). all epitopes were located in the region corresponding to the major capsid protein vp2. based on this information, and on analogy to other autonomous parvoviruses, 24 different peptides were synthesised, coupled to keyhole ...19989620208
plum pox potyvirus resistance associated to transgene silencing that can be stabilized after different number of plant generations.nicotiana benthamiana plants were transformed with a fragment of the plum pox potyvirus (ppv) genome that encodes the nuclear inclusion a (nia) and b (nib) proteins and the n-terminus of the capsid protein (nia-nib-cp). lines transformed with this ppv genomic fragment harboring mutations in the gdd replicase-motif were also obtained. plants of niadeltav lines that carry a gdd to vdd mutation in the ppv transgene, were immune to ppv infection. the resistance was highly specific, since it was only ...19989469941
ultrastructural localization of nonstructural and coat proteins of 19 potyviruses using antisera to bacterially expressed proteins of plum pox potyvirus.antisera to the bacterially expressed nonstructural proteins (nsp) hc-pro, ci, nia, and nib and the coat protein (cp) of plum pox potyvirus (ppv) were used for analysing the composition of virus-induced cytoplasmic and nuclear inclusions by electron microscopy. the antisera reacted with nsp and cp of ppv on immunogold-labelled ultrathin sections. antiserum to cp reacted with virions of seven out of 18 other potyviruses. cp was distributed throughout the cytoplasm of infected cells. antisera to p ...19989856098
nicotiana benthamiana plants transformed with the plum pox virus helicase gene are resistant to virus infection.nicotiana benthamiana domin. plants were transformed with the cytoplasmic inclusion protein (ci) gene of plum pox potyvirus (ppv) to investigate, whether this non-structural protein would be able to confer resistance. the ci protein is an rna helicase, which contains a conserved nucleotide binding motif (ntbm) and plays an important role in viral replication. two gene constructions were developed for plant transformation. the first contains the original coding sequence of the ci gene under the c ...19989617773
development of an antigen presentation system based on plum pox potyvirus.the development of an antigen presentation system based on the plum pox potyvirus (ppv) is here described. the amino-terminal part of ppv capsid protein was chosen as the site for expression of foreign antigenic peptides. modifications in this site were engineered to avoid the capability of natural transmission by aphids of this ppv vector. as a first practical attempt, different forms of an antigenic peptide (single and tandem repetition) from the vp2 capsid protein of canine parvovirus (cpv) w ...19989607317
routine diagnosis of herpes simplex virus (hsv) encephalitis by an internal dna controlled hsv pcr and an igg-capture assay for intrathecal synthesis of hsv antibodies.the development of antiviral therapy increases the need for rapid, sensitive and reliable methods or combination of methods for diagnosis and monitoring herpes simplex encephalitis, hse.19989562858
comparison of monoclonal antibodies and polymerase chain reaction assays for the typing of isolates belonging to the d and m serotypes of plum pox potyvirus.abstract plum pox potyvirus (ppv) isolates may be divided into four groups separated by serological, molecular, and epidemiological differences. monoclonal antibodies specific for the two major groups of isolates, represented by the d and m serotypes of the virus, have been obtained. polymerase chain reaction (pcr)-based assays allowing the direct detection and differentiation of ppv isolates have also been developed. we now report on a large-scale comparison of these two typing approaches. the ...199818944965
enumeration of marine viruses in culture and natural samples by flow cytometryflow cytometry (fcm) was successfully used to enumerate viruses in seawater after staining with the nucleic acid-specific dye sybr green-i. the technique was first optimized by using the phaeocystis lytic virus ppv-01. then it was used to analyze natural samples from different oceanic locations. virus samples were fixed with 0.5% glutaraldehyde and deep frozen for delayed analysis. the samples were then diluted in tris-edta buffer and analyzed in the presence of sybr green-i. a duplicate sample ...19999872758
engineering parvovirus-like particles for the induction of b-cell, cd4(+) and ctl responses.an antigen delivery system based on hybrid recombinant parvovirus-like particles (vlps) formed by the self-assembly of the capsid vp2 protein of porcine (ppv) or canine parvovirus (cpv) expressed in insect cells with the baculovirus system has been developed. ppv:vlps containing a cd8(+) epitope from the lcmv nucleoprotein evoked a potent ctl response and were able to protect mice against a lethal infection with the virus. also, ppv:vlps containing the c3:t epitope from poliovirus elicited a cd4 ...199910506659
use of parvovirus-like particles for vaccination and induction of multiple immune responses.expression of the vp2 gene of autonomous parvoviruses in insect cells with the use of the baculovirus system has led to the production of virus-like particles (vlps) formed by the self-assembly of vp2. these vlps are expressed at high levels and can easily be purified by salt fractionation. they are highly immunogenic in the corresponding host, being fully protective at doses as low as 1-2 microg of purified material per animal. no special adjuvants are required. an interesting property of these ...199910075910
[comparative investigations of a combined vaccine against parvovirus and erysipelas and corresponding monovaccines in different vaccination schedules. 1: field trial].in a field trial, the development of antibodies of a combined vaccine against the porcine parvovirus (ppv) as well as against swine erysipelas was compared with corresponding mono vaccines. furthermore, these vaccines were used in different vaccination schedules. the tests were carried out on 109 gilts in three closed farms. in all gilts, a basic immunization repeated twice was carried out at the age of six months and at intervals of three weeks. the revaccination was carried out four months aft ...199910384706
reproduction of lesions of postweaning multisystemic wasting syndrome in gnotobiotic piglets.neonatal gnotobiotic piglets were inoculated with tissue homogenates and low- and high-passage cell culture material to determine if the lesions of the newly described porcine postweaning multisystemic wasting syndrome (pmws) could be reproduced. for this, 17 3-day-old gnotobiotic piglets were inoculated intranasally with pelleted chloroform-treated, filtered extracts from cell cultures, filter-sterilized homogenates of lymphoid tissue from pmws-affected piglets, or control materials. piglets we ...19999925205
mitotic stability of infection-induced resistance to plum pox potyvirus associated with transgene silencing and dna methylation.plum pox potyvirus (ppv) infection of transgenic nicotiana benthamiana plants that expressed the ppv nib rna replicase carrying a gly to val mutation at the gdd motif (nibv lines) induced a phenotype of virus resistance and transgene silencing, which was not transmissible to the progeny after self-fertilization (h. s. guo and j. a. garcía, mol. plant-microbe interact. 10:160-170, 1997). here, we demonstrate that the induced resistance of nibv plants is mitotically stable after plant propagation ...19999926412
molecular and structural basis of the evolution of parvovirus tropism.parvoviruses have small genomes and, consequently, are highly dependent on their host for various functions in their reproduction. since these viruses generally use ubiquitous receptors, restrictions are usually intracellularly regulated. a lack of mitosis, and hence absence of enzymes required for dna replication, is a powerful block of virus infection. allotropic determinants have been identified for several parvoviruses: porcine parvovirus, canine parvovirus (cpv), feline parvovirus (feline p ...199910497831
virus safety of avital bone tissue transplants: evaluation of sterilization steps of spongiosa cuboids using a peracetic acid-methanol mixture.the aim of this study was to validate the virus-inactivating/eliminating capacity of the manufacturing process of spongiosa cuboids. both the sterilization step with peracetic acid (paa)/ethanol and the defatting step of bones with chloroform/methanol (2:1, v/v) were investigated. relevant enveloped, non-enveloped, and model viruses belonging to different virus families were included in the investigation: human immunodeficiency virus type 2 (hiv-2), hepatitis a virus (hav), poliovirus (pv-1), ps ...199910652175
preparation of diagnostic monoclonal antibodies against two potyviruses.diagnostic monoclonal and polyclonal antibodies against bean yellow mosaic virus (bymv) and plum pox virus (ppv) were prepared, characterized and used for detection of these viruses in infected plants by enzyme-linked immunosorbent assay (elisa), immunoblot analysis and tissue print immunoblot assay (tpiba).199910749372
plum pox virus capsid protein mobility in sds-polyacrylamide gel electrophoresis.slovak plum pox virus (ppv) isolates bor-3 and kr-4 behaved atypically in polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate (sds-page) of their capsid proteins (cps). whereas other tested ppv isolates could be sorted by sds-page into groups corresponding to the m or d strains, as controlled by restriction fragment length polymorphism (rflp) analysis, the two above mentioned isolates behaved as the m strain according to rflp analysis but not sds-page. slight mobility di ...199910749373
monoclonal antibodies to the gp5 of porcine reproductive and respiratory syndrome virus are more effective in virus neutralization than monoclonal antibodies to the gp4.the arterivirus porcine reproductive and respiratory syndrome virus (prrsv) contains six structural proteins the roles of which are not completely understood. in a preceding study, immunization with the dutch isolate i10 of prrsv had led to the development of mabs against four structural proteins [wieczorek-krohmer, m., 1994. herstellung und charakterisierung von monoklonalen antikörpern gegen das virus des porzinen reproduktiven und respiratorischen syndroms (prrsv). inaugural-dissertation, lud ...199910227120
intranasal delivery of recombinant parvovirus-like particles elicits cytotoxic t-cell and neutralizing antibody responses.we previously demonstrated that chimeric porcine parvovirus-like particles (ppv:vlp) carrying heterologous epitopes, when injected intraperitoneally into mice without adjuvant, activate strong cd4(+) and cd8(+) t-cell responses specific for the foreign epitopes. in the present study, we investigated the immunogenicity of ppv:vlp carrying a cd8(+) t-cell epitope from the lymphocytic choriomeningitis virus (lcmv) administered by mucosal routes. mice immunized intranasally with recombinant ppv:vlp, ...199910074120
parapoxviruses: potential alternative vectors for directing the immune response in permissive and non-permissive hosts.parapoxvirus (ppv) represents a genus of the poxviridae, and particularly ppv ovis (orf virus, ov) seems to offer several potential advantages for the use of vector vaccine. therefore, we started to investigate the genome of the highly attenuated ov strain d1701, which was only poorly characterised until now. due to recombination of non-homologous sequences, part of the right hand end of the d1701 genome was duplicated and translocated to the opposite end of the genome. as a consequence gene del ...199910486932
detection of porcine parvovirus dna by the polymerase chain reaction assay using primers to the highly conserved nonstructural protein gene, ns-1.porcine parvovirus (ppv) infection is associated with reproductive losses in swine and its causative agent, the ppv, has been isolated worldwide. serological surveys and virus isolation studies throughout brazil confirm the occurrence of ppv infection in this country. the most common methods to detect ppv infection are fluorescent antibody staining of fetal tissues, hemagglutination assay of tissue extracts and virus isolation from fetal tissues. non-specificity and low sensitivity are the major ...199910204709
experimental reproduction of severe wasting disease by co-infection of pigs with porcine circovirus and porcine parvovirus.colostrum-deprived pigs were infected intranasally with a recent isolate of porcine circovirus (pcv2) and a porcine parvovirus (ppv), both from canadian pigs with post-weaning multisystemic wasting syndrome (pmws). four pigs were inoculated with pcv2 alone, three with ppv alone, five with a combined pcv2/ppv inoculum, and two with a chloroform-treated combined pcv2/ppv inoculum. pigs were killed 21-26 days after infection and tissue samples examined for gross and microscopical lesions and for th ...199910373289
myocarditis and abortion associated with intrauterine infection of sows with porcine circovirus 2.porcine circovirus 2 (pcv2) is a recently identified agent that has been associated with postweaning multisystemic wasting syndrome (pmws) in swine populations. in this report, the potential spectrum of disease associated with pcv2 is expanded by evidence of vertical transmission and associated reproductive failure. pcv2 was isolated from a litter of aborted piglets from a farm experiencing late-term abortions and stillbirths. severe, diffuse myocarditis was present in 1 piglet associated with e ...199912968736
localization of plum pox virus in stem and petiole tissues of apricot cultivars by immuno-tissue printing.localization of plum pox virus (ppv) in stem and petiole tissues of four susceptible and four resistant apricot cultivars has been studied. consecutive 1-mm spaced transverse sections were taken from the tissues and were printed onto nitrocellulose membrane in duplicate. for virus-specific detection, one series of prints was probed with an antibody to ppv coat and the density of stains was evaluated by light microscopy. another series of prints was treated with a substrate but not with the antib ...200011332274
efficiency of inoculation of peach gf305 seedlings with plum pox virus by different methods.peach gf305 is frequently used as rootstock in experiments to evaluate the resistance of different species of prunus to plum pox virus (ppv) because of its extreme susceptibility. however, transmission of ppv in prunus species is sometimes problematic due to its low concentration or uneven distribution in these species. to determine the most effective way of transmitting the virus, different infection methods (by aphids, grafting, mechanical infection and injection) were tested using dideron ppv ...200011332275
experimental infection of colostrum deprived piglets with porcine circovirus 2 (pcv2) and porcine reproductive and respiratory syndrome virus (prrsv) potentiates pcv2 replication.experimental infection of colostrum-deprived (cd) pigs with a combined inoculum of porcine circovirus 2 (pcv2) and porcine reproductive and respiratory syndrome virus (prrsv) potentiated the replication and distribution of pcv2 virus, when compared with pigs inoculated with pcv2 alone. the replication and distribution of prrsv in dually infected pigs was not enhanced, when compared to pigs inoculated with prrsv alone. the mechanisms involved in the potentiation of pcv2 replication in pcv2/prrsv ...200011205128
evaluation and validation of virus removal by ultrafiltration during the production of diaspirin crosslinked haemoglobin (dclhb).virus retention during ultrafiltration through a/g technology filter cartridges was investigated to characterize the removal process and validate the degree of virus titre reduction during the filtration of red blood cell haemolysates performed as part of the production of diaspirin crosslinked haemoglobin (dclhb). when viruses were suspended in phosphate buffered saline solution, retention was greater with larger sized viruses and smaller filter pore size. virus titre was maintained at starting ...200010885615
episomal expression of a hammerhead ribozyme directed against plum pox virus.two related antisense rnas directed against plum pox virus (ppv) were expressed episomally in nicotiana clevelandii by infection with recombinant potato virus x (pvx). one recombinant pvx expressed an ordinary ppv antisense rna of about 400 nucleotides, while the other expressed a related antisense rna that carried the catalytic domain of a hammerhead ribozyme. inoculation with the latter recombinant pvx resulted in the accumulation of ribozyme rna that was catalytically active when tested in vi ...200010930659
sensitivity, specificity, and positive predictive value of oral opportunistic infections in adults with hiv/aids as markers of immune suppression and viral burden.the purpose of this study was to assess the use of human immunodeficiency virus (hiv)-related oral opportunistic infections as markers of immune suppression and viral burden in adults with hiv/acquired immunodeficiency syndrome (aids).200010936837
effect of different baculovirus inactivation procedures on the integrity and immunogenicity of porcine parvovirus-like particles.we have demonstrated earlier the usefulness of recombinant porcine parvovirus (ppv) virus-like particles (vlps) as an efficient recombinant vaccine for ppv. here, we have demonstrated that preparations of ppv vlps could be contaminated by recombinant baculoviruses. since these baculoviruses can be a problem for the registration and safety requirements of the recombinant vaccine, we have tested different baculovirus inactivation strategies, studying simultaneously the integrity and immunogenicity ...200011115693
preservation of 5'-end integrity of a potyvirus genomic rna is not dependent on template specificity.full-length in vitro transcripts of plum pox potyvirus (ppv) genomic rna with mutations altering the number of 5'-terminal adenosine residues were able to infect nicotiana clevelandii plants, whereas a mutant with a substitution of adenosine in position 2 by guanosine failed to infect. the genomic 5' end was template-independently repaired during in vivo rna synthesis producing wild-type viral progeny. putative models of replication initiation are discussed.200010753716
detection of human cytomegalovirus pp67 late gene transcripts in cerebrospinal fluid of human immunodeficiency virus type 1-infected patients by nucleic acid sequence-based amplification.this study examined the clinical correlation between the presence of human cytomegalovirus (hcmv) pp67 mrna in cerebrospinal fluid (csf) and active hcmv central nervous system (cns) disease in patients with human immunodeficiency virus type 1 (hiv-1). in total, 76 csf specimens collected from 65 hiv-1-positive patients diagnosed with hcmv cns disease, other non-hcmv-related cns diseases, or no cns disease were tested for the presence of hcmv pp67 mrna using the nuclisens cytomegalovirus (cmv) pp ...200010790122
viral wasting syndrome of swine: experimental reproduction of postweaning multisystemic wasting syndrome in gnotobiotic swine by coinfection with porcine circovirus 2 and porcine parvovirus.one-day-old gnotobiotic piglets were inoculated intranasally with in vitro passaged porcine circovirus 1 (pcv-1), pcv-2, and porcine parvovirus (ppv) alone or in combination (pcv-1/pcv-2, pcv-1/ppv, and pcv-2/ppv). piglets were evaluated for 1) the development of porcine postweaning multisystemic wasting syndrome (pmws), 2) distribution of viral antigens by immunochemistry, and 3) viremia and the presence of viral dna in nasal and ocular secretions and feces. all single agent-infected piglets an ...200010810990
detection of porcine enteroviruses by nrt-pcr: differentiation of cpe groups i-iii with specific primer sets.porcine enteroviruses (pev) comprising at least 13 serotypes grouped into three species are described as causative agents of neurological disorders, fertility disorders, and dermal lesions of swine. despite their well-documented acid stability, enteric infection route, and similarity of clinical symptoms, most of the porcine enterovirus (pev) serotypes are set apart from the genus enterovirus of the picornaviridae. hence, pcr procedures used commonly to detect enteroviruses are not applicable to ...200010960708
generation of recombinant parapoxviruses: non-essential genes suitable for insertion and expression of foreign genes.orf virus (ov) is an epitheliotropic poxvirus and belongs to the genus parapoxvirus (ppv). ppv, especially ov, is regarded as a promising candidate for an expression vector. among available live vaccines only strain d1701 represents a highly attenuated ov strain with clearly reduced pathogenicity. therefore, we started to identify potentially non-essential genes or regions of d1701, which might be suitable for insertion and expression of foreign genes. the present contribution reviews some of th ...200011000469
experimental transmission of porcine circovirus type 2 (pcv2) in weaned pigs: a sequential study.weaned specific pathogen-free pigs were inoculated intranasally with porcine circovirus type 2 (pcv2) and killed in groups of two or three animals at 6, 13, 20, 27 and 34 days post-inoculation (dpi), together with appropriate uninfected controls, for examination by histopathological, immunohistochemical (immunogold silver staining; igss), polymerase chain reaction (pcr) and viral isolation techniques. serum samples were also collected for detection of antibodies. no major clinical signs were obs ...200011041995
distribution of porcine parvovirus in porcine circovirus 2-infected pigs with postweaning multisystemic wasting syndrome as shown by in-situ hybridization.in-situ hybridization with a nonradioactive digoxigenin-labelled probe was used to study the distribution of porcine parvovirus (ppv) in formalin-fixed paraffin wax-embedded tissues from 10 porcine circovirus 2 (pcv2)-infected weaned pigs with naturally occurring postweaning multisystemic wasting syndrome (pmws). a 226 base pair dna fragment from a vp2 structural gene was generated by polymerase chain reaction (pcr) and used as a probe. lymph node, spleen, thymus and tonsil were positive by pcr, ...200011042001
combined pap smear, cervicography and hpv dna testing in the detection of cervical intraepithelial neoplasia and cancer.the sensitivity of the pap smear (pap) continues to be the subject of debate. during the past several years, cervicography (cer) and hpv dna testing have been suggested as optional tools in the screening of cervical cancer precursors.200010833984
in vivo induction of a high-avidity, high-frequency cytotoxic t-lymphocyte response is associated with antiviral protective immunity.many approaches are currently being developed to deliver exogenous antigen into the major histocompatibility complex class i-restricted antigen pathway, leading to in vivo priming of cd8(+) cytotoxic t cells. one attractive possibility consists of targeting the antigen to phagocytic or macropinocytic antigen-presenting cells. in this study, we demonstrate that strong cd8(+) class i-restricted cytotoxic responses are induced upon intraperitoneal immunization of mice with different peptides, chara ...200010846055
the use of transgenic fruit trees as a resistance strategy for virus epidemics: the plum pox (sharka) model.sharka or plum pox, caused by plum pox virus (ppv: genus potyvirus; family potyviridae), is the most serious disease of prunus. most cultivated prunus species are highly susceptible and conventional breeding has not produced highly resistant and commercially acceptable varieties. success in developing virus-resistant herbaceous crops through genetic engineering led us to investigate this approach for resistance to ppv. our programme aims to develop a biotechnological approach to ppv control that ...200011137162
porcine circoviruses: a review.porcine circoviruses (pcv) are small nonenveloped dna viruses containing a unique single-stranded circular genome. previously, no recognized link was found between pcv infection of pigs and disease, and pcv was considered a nonpathogenic agent. over the last 5 years, a "novel" pcv, designated pcv2, has been associated with various disease syndromes in pigs, primarily postweaning multisystemic wasting syndrome (pmws). pigs with pmws have a variety of clinical signs, including debility, dyspnea, p ...200010690769
coinfection by porcine circoviruses and porcine parvovirus in pigs with naturally acquired postweaning multisystemic wasting syndrome.postweaning multisystemic wasting syndrome (pmws) is an emerging disease in swine. recently, the disease has been reproduced with inocula containing a newly described porcine circovirus (pcv), designated pcv 2, and porcine parvovirus (ppv). in order to determine if these viruses interact in naturally acquired pmws, affected tissues from field cases were examined by immunohistochemistry (ihc) and polymerase chain reaction (pcr) for pcv 2 and ppv, as well as by pcr for the other recognized porcine ...200010690771
comparative analysis of dna flow cytometry and cytology of bladder washings: review of discordant cases.dna analysis is becoming an important diagnostic and prognostic adjunct test in urinary cytology. the aim of this study was to compare the results of dna flow cytometry (fcm) with the cytologic diagnosis of bladder washings (bw). dna ploidy was evaluated in 251 bw. in 65 cases, follow-up surgical biopsies were available. cytology results were classified as positive and negative, and fcm results were categorized as diploid and aneuploid. both tests were evaluated independently. cases were defined ...200010649514
investigation of porcine parvovirus among persons with hemophilia receiving hyate:c porcine factor viii concentrate.porcine clotting factor has been used for more than 15 years to treat severe bleeding episodes in persons with hemophilia who have antibodies to human clotting factor. in 1996, qc procedures revealed for the first time the presence of porcine parvovirus (ppv) in the product. this report describes an investigation to determine the extent of product contamination and to evaluate past recipients of porcine clotting factor (hyate:c, speywood biopharm) for evidence of ppv infection.200010864993
transgenic or plant expression vector-mediated recombination of plum pox virus.different mutants of an infectious full-length clone (p35ppv-nat) of plum pox virus (ppv) were constructed: three mutants with mutations of the assembly motifs rq and df in the coat protein gene (cp) and two cp chimeras with exchanges in the cp core region of zucchini yellow mosaic virus and potato virus y. the assembly mutants were restricted to single infected cells, whereas the ppv chimeras were able to produce systemic infections in nicotiana benthamiana plants. after passages in different t ...200010906199
occupational risk of infection by varicella zoster virus in belgian healthcare workers: a seroprevalence study.to assess the prevalence of varicella zoster virus (vzv) antibodies in flemish (belgian) healthcare workers, to investigate the association between seronegativity and selected variables, and to assess the reliability of recall about disease as a predictor of immunity.200010935943
native electrophoresis and western blot analysis (neweb): a method for characterization of different forms of potyvirus particles and similar nucleoprotein complexes in extracts of infected plant tissues.a combination of native electrophoresis and immunodetection (western blot) was used for the characterization of nucleoprotein particles of the potyvirus plum pox virus (ppv). virus particles were electrophoresed directly from plant extracts in agarose or mixed acrylamide-agarose gels under native conditions, blotted on nitrocellulose membranes, and characterized with the aid of a coat protein-specific antibody. using this combined methodology, called neweb (native electrophoresis and western blo ...200010950988
a sequential study of experimental infection of pigs with porcine circovirus and porcine parvovirus: immunostaining of cryostat sections and virus isolation.the sequential tissue distribution of virus was investigated using virus isolation and immunofluorescence tests in 1-day-old piglets inoculated with porcine circovirus 2 (pcv2) and/or porcine parvovirus (ppv). enlarged mesenteric lymph nodes were seen in the pig inoculated with pcv2 alone and killed at 26 days post-inoculation (pi). one of the pigs inoculated with pcv2 and ppv and killed at 21 days pi had an enlarged liver. the pig killed at 26 days pi in this group had enlarged liver, kidneys a ...200010763376
biotechnological aspects of plum pox virus.plum pox potyvirus (ppv), the causal agent of a devastating disease that affects stone fruit trees, is becoming a target of intense studies intended both to fight against viral infection and to develop practical applications based on the current knowledge of potyvirus molecular biology. this review focuses on biotechnological aspects related to ppv, such as novel diagnostic techniques that facilitate detection and typing of virus isolates, strategies to implement pathogen-derived resistance thro ...200010656327
identification of a pathogenicity determinant of plum pox virus in the sequence encoding the c-terminal region of protein p3+6k(1).a full-length genomic cdna clone of a plum pox potyvirus (ppv) isolate belonging to the m strain (ppv-ps) has been cloned downstream from a bacteriophage t7 polymerase promoter and sequenced. transcripts from the resulting plasmid, pgppvps, were infectious and, in herbaceous hosts, produced symptoms that differed from those of virus progeny of pgppv, a full-length genomic cdna clone of the d strain ppv-r. viable ppv-r/-ps chimeric viruses were constructed by recombination of the cdna clones in v ...200010675393
the effect of porcine parvovirus and porcine reproductive and respiratory syndrome virus on porcine reproductive performance.from a worldwide perspective, porcine parvovirus (ppv) and porcine reproductive and respiratory syndrome virus (prrsv) are the most common viral causes of porcine reproductive failure. a typical epidemic of ppv-induced reproductive failure is presented as an increased number of mummified fetuses and sometimes, entire litters are mummified. if infection with ppv is very early in gestation, the number of liveborn pigs may be further reduced as a result of embryonic death and resorption. during the ...200010844195
trial-of-antibiotic algorithm for the diagnosis of tuberculosis in a district hospital in a developing country with high hiv prevalence.to evaluate a diagnostic algorithm for pulmonary tuberculosis based on smear microscopy and objective response to trial of antibiotics.200010864181
mutations in the coat protein gene of plum pox virus suppress particle assembly, heterologous encapsidation and complementation in transgenic plants of nicotiana benthamiana.two different motifs in the coat protein (cp) of plum pox virus (ppv) (r(3015)q(3016), d(3059)) were mutated by replacing the respective amino acids with others possessing different chemical properties. the mutated cp genes were introduced into an infectious full-length clone of ppv (p35ppv-nat) to investigate their influence on systemic infection of transgenic wild-type ppv cp-expressing and non-transgenic plants of nicotiana benthamiana. all mutants failed to establish systemic infections in n ...200010675394
reproduction of lesions of postweaning multisystemic wasting syndrome by infection of conventional pigs with porcine circovirus type 2 alone or in combination with porcine parvovirus.post-weaning multisystemic wasting syndrome (pmws) has recently emerged as an important disease of pigs in north america, europe and asia. porcine circovirus type 2 (pcv2) and porcine parvovirus (ppv) have been isolated from affected pigs. to investigate the pathogenicity of these isolates, groups of colostrum-deprived conventional pigs were inoculated with pcv2 alone (n=4), ppv alone (n=3) or dually with pcv2 and ppv (n=7) and examined post mortem between 21 and 26 days post-infection (dpi). tw ...200010627387
protection of rabbits against rabbit hemorrhagic disease virus by immunization with the vp60 protein expressed in plants with a potyvirus-based vector.a new plum pox potyvirus (ppv)-based vector has been constructed for the expression of full-length individual foreign proteins. the foreign sequences are cloned between the nib replicase and capsid protein (cp) cistrons. the heterologous protein is split from the rest of the potyviral polyprotein by cleavage at the site that originally separated the nib and cp proteins and at an additional nia protease recognition site engineered at its amino-terminal end. this vector (ppv-nk) has been used to c ...200111162842
a viral phospholipase a2 is required for parvovirus infectivity.sequence analysis revealed phospholipase a2 (pla2) motifs in capsid proteins of parvoviruses. although pla2 activity is not known to exist in viruses, putative pla2s from divergent parvoviruses, human b19, porcine parvovirus, and insect gmdnv (densovirus from galleria mellonella), can emulate catalytic properties of secreted pla2. mutations of critical amino acids strongly reduce both pla2 activity and, proportionally, viral infectivity, but cell surface attachment, entry, and endocytosis by pla ...200111702787
clinical diagnosis of influenza virus infection: evaluation of diagnostic tools in general practice.with the development of new antiviral agents for influenza, the urge for rapid and reliable diagnosis of influenza becomes increasingly important. respiratory virus infections are difficult to distinguish on clinical grounds. general practitioners (gps) however, still depend on their clinical judgement.200111510391
experimental reproduction of severe disease in cd/cd pigs concurrently infected with type 2 porcine circovirus and porcine reproductive and respiratory syndrome virus.three-week-old cesarean-derived colostrum-deprived (cd/cd) pigs were inoculated with porcine circovirus type 2 (pcv2, n = 19), porcine reproductive and respiratory syndrome virus (prrsv, n = 13), concurrent pcv2 and prrsv (pcv2/prrsv, n = 17), or a sham inoculum (n = 12) to compare the independent and combined effects of these agents. necropsies were performed at 7, 10, 14, 21, 35, and 49 days postinoculation (dpi) or when pigs became moribund. by 10 dpi, pcv2/prrsv-inoculated pigs had severe dy ...200111572560
clinical and pathological observations on pigs with postweaning multisystemic wasting syndrome.the aim of this work was to characterise the lesions and agents present in clinically normal and clinically affected pigs on a farm during an outbreak of postweaning multisystemic wasting syndrome (pmws), and to evaluate the diagnostic techniques for detecting porcine circovirus type 2 (pcv-2) and other microorganisms. four pigs in the early stage and 11 pigs in the late stage of the disease, and eight clinically normal pigs were necropsied. samples of lymphoid tissue and serum were also obtaine ...200111594382
identification of an n-terminal domain of the plum pox potyvirus ci rna helicase involved in self-interaction in a yeast two-hybrid system.potyvirus ci rna helicase is a protein involved in rna genome replication and virus movement. the protein aggregates in the cytoplasm of infected cells to form typical cylindrical inclusions. a yeast two-hybrid system was used to analyse interactions of the ci rna helicase from plum pox potyvirus (ppv) with itself and with other viral proteins. no interactions could be detected of full-length ci protein with itself or with ppv p3/6k1, nia, nib or cp proteins. however, positive self-interactions ...200111172111
integrated rt-pcr/nested pcr diagnosis for differentiating between subgroups of plum pox virus.an rt-pcr/nested pcr technique was developed for the simultaneous detection and typing of plum pox virus (ppv) and its major types--dideron (d), marcus (m), el-amar (ea) and cherry (c). degenerated oligonucleotides were synthesized for the general detection of ppv, flanking the coding sequence for the n-terminal portion of the coat protein (cp), within which strain-specific differences were identified. on the basis of these characteristic differences, degenerated primer pairs were designed to di ...200111226563
pathogenicity determinants in the complex virus population of a plum pox virus isolate.several subisolates were separated from a single plum pox virus (ppv) isolate, ppv-ps. in spite of an extremely high sequence conservation (more than 99.9% similarity), different subisolates differed largely in pathogenicity in herbaceous hosts and infectivity in woody plants. the severity of symptomatology did not seem to correlate with virus accumulation. sequence analysis and site-directed mutagenesis demonstrated that single amino acid changes in the helper component (hc) protein caused a dr ...200111277425
incidence of pulmonary tuberculosis and hiv coinfection in the province of seville, spain, 1998.using the national notifiable disease surveillance system (nndss) and minimum basic data set (mbds) for hospital discharges we evaluated pulmonary tuberculosis (ptb) surveillance and estimated real ptb incidence and human immunodeficiency virus (hiv) coinfection in seville in 1998. in addition, we assessed the sensitivity and the positive predictive value (ppv) of the surveillance system. real incidence of pulmonary tuberculosis was estimated by the capture-recapture method. in 1998, the provinc ...200112086091
response of antioxidative enzymes to plum pox virus in two apricot cultivars.recent evidence has indicated that activated oxygen species (aos) may function as molecular signals in the induction of defence genes. in the present work, the response of antioxidative enzymes to the plum pox virus (ppv) was examined in two apricot (prunus armeniaca l.) cultivars, which behaved differently against ppv infection. in the inoculated resistant cultivar (goldrich), a decrease in catalase (cat) as well as an increase in total superoxide dismutase (sod) and dehydroascorbate reductase ...200111240915
post-transcriptional gene silencing in plum pox virus resistant transgenic european plum containing the plum pox potyvirus coat protein gene.transgenic plums containing the plum pox potyvirus coat protein (ppv-cp) gene were inoculated with ppv. infection was monitored by evaluating symptoms, elisa, and ic-rt-pcr. transgenic clone c5 was highly resistant to ppv during four years of testing and displayed characteristics typical of post-transcriptional gene silencing (ptgs), including a high level of transgene transcription in the nucleus, low levels of transgene mrna in the cytoplasm, a complex multicopy transgene insertion with aberra ...200111437277
virological response to interferon therapy of chronic hepatitis b as measured by a highly sensitive assay.in the interferon (ifn) treatment of chronic hepatitis b, there is no accepted definition of virological response as measured by highly sensitive hbv dna assays. in the present study of 98 patients given ifn (10 mu/day for 1 week, then 10 mu tiw for 11 weeks) with or without prednisolone priming, a virological response was identified as log hbv dna/ml below 6.0 (by amplicor monitor, roche) 6 months post-treatment. at this time, 92% (33/36) of the sustained responders (sr) still had detectable vi ...200111555192
simultaneous detection of porcine circovirus type 2 and porcine parvovirus in pigs with pmws by multiplex pcr. 200111570792
budding of fowlpox and pigeonpox viruses at the surface of infected cells.chick embryo fibroblasts and chorioallantoic membranes infected with fowlpox virus (fwpv) or pigeonpox virus (ppv) were examined by transmission and scanning electron microscopy. immature virus particles were observed in finely granular areas, i.e. virus factories, of the cytoplasm. these particles had various forms depending on their stages of development. many tubular structures were also seen in these regions. mature virus particles with ellipsoidal or brick-shaped forms enclosing electron-de ...200111347712
prevalence of porcine reproductive and respiratory syndrome virus, porcine circovirus type 2 and porcine parvovirus from aborted fetuses and pigs with respiratory problems in korea.porcine reproductive and respiratory syndrome virus(prrsv)0, porcine circovirus type 2(pcv-2) and porcine parvovirus (ppv)0 infections were investigated as possible causes of the postweaning multisystemic wasting syndrome(pmws). specific primers for rt-pcr and pcr were designed for the differential detection of prrsv, pcv-2 and ppv. using pcr, these viruses were detected in homogenized tissue samples from pigs that had respiratory of reproductive problems in the time period between 1998 and 2000 ...200112441689
pilot study of expedited hiv-1 testing of women in labor at an inner-city hospital in new york city.to evaluate the feasibility of offering voluntary counseling and expedited human immunodeficiency virus (hiv) testing to women in labor, and to assess the characteristics of two rapid hiv assays compared with results from an expedited standard enzyme immunoassay (eia), with western blot confirmation, as indicated, we undertook a pilot study immediately prior to enactment of new york state regulations (august 1999) requiring expedited testing of laboring women (or newborns) with undocumented hiv ...200111321245
comparison of the efficacy of virus inactivation methods in allogeneic avital bone tissue transplants.several procedures for inactivating viruses are used presently in the context of bone tissue transplants. common methods used are gamma irradiation (25 kgy), treatment with moist heat (82.5 degrees c/15 min., lobator-sd2-system) as well as chemical sterilisation using peracetic acid-ethanol treatment (pes, 2% peracetic acid, 96% ethanol, aqua [2:1:1], 200 mbar, agitation, 4 hours). based on national and international guidelines, we tested the antivirucidal effectiveness of these methods in human ...200115256903
identification of plum pox virus determinants implicated in specific interactions with different prunus spp.abstract the characterization of pathogenic properties of two infectious clones of plum pox virus (ppv) isolates, pgppv (d group) and pgppvps (m group), was investigated in their woody hosts (seedlings of prunus spp.). the two clones differed in their ability to infect plum and peach cultivars, from no infection to local and systemic infection. the phenotype determinants were located with a set of chimeric viruses from the two clones. in plum, determinants of systemic infection were located in a ...200118944389
the production of a genus-specific recombinant antibody (scfv) using a recombinant potyvirus protease.a single chain variable fragment antibody (scfv; anti-nia scfv102) was selected from a synthetic human antibody library by using a nia protease of plum pox virus (ppv) as an antigen, which was expressed in bacteria. the nia protease forms the nuclear inclusion body a and acts as the major protease in the cleavage of the viral polyprotein into functional proteins. the nia protein was detected with anti-nia scfv102 after expression in escherichia coli cells as well as from ppv-infected nicotiana b ...200212393153
broad-spectrum virus reduction in red cell concentrates using inactine pen110 chemistry.the risk of transmission of blood-borne pathogens by transfusion is a persistent problem in medicine. to address this safety issue, inactine pen110 chemistry is being utilized to develop a process for preparing pathogen-reduced red blood cell concentrates (rbcc). the purpose of this study was to characterize the virucidal effectiveness of the inactine pen110 chemistry in full units of rbcc by using a panel of viruses with diverse properties in composition, size and shape.200212437518
genetic linkage maps of two apricot cultivars ( prunus armeniaca l.), and mapping of ppv (sharka) resistance.genetic linkage maps for two apricot cultivars have been constructed using aflp, rapd, rflp and ssr markers in 81 f1 individuals from the cross 'goldrich' x 'valenciano'. this family segregated for resistance to 'plum pox virus' (ppv), the most-important virus affecting prunus species. of the 160 rapd arbitrary primers screened a total of 44 were selected. sixty one polymorphic rapd markers were scored on the mapping population: 30 heterozygous in 'goldrich', 19 heterozygous in 'valenciano', seg ...200212582518
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