cell-mediated immunity to leukemia virus- and tumor-associated antigens in mice.cell-mediated immune reactions appear to play an important role in resistance against growth of leukemia cells in mice. possible mechanisms for in vivo protection in two tumor systems are discussed. these tumor models, which are a friend leukemia virus-induced transplantable tumor, fbl-3, and primary murine sarcoma virus (msv) -induced tumors, are strongly antigenic; under some conditions, tumors regress completely. in mice with regressing fbl-3 tumors, cell-mediated cytotoxicity was measured by ...197656223
feline panleukopaenia virus and mink enteritis virus. a serological study. 197766862
passage of red blood cells through the sinusoidal wall of the spleen.the migration of blood cells across the wall of splenic sinusoids has been studied by means of serial sections in rats inoculated with murine erythroblastosis virus (mev). the sinusoidal walls of the normal spleen have no permanent openings. in the normal spleen, mature red blood cells and blood cells in the latest stages of maturation can be seen in diapedesis. the predominant form of transmural migration is intercellular. in mev-inoculated animals, in which there is a vigorous erythroblastic p ...1975164765
derivation of mouse sarcoma virus (kirsten) by acquisition of genes from heterologous host.the technique of virus rna-cellular dna hybridization in solution with dna excess was used to compared the nucleotide sequences of the 70 s rna genome of the kirsten mouse sarcoma virus (ki-msv) with that of mouse erythroblastosis virus (mev) which gave rise to ki-msv after in vivo propagation in rat. it is suggested that a loss of about 30% of the genomic sequences of mev with a concomitant gain of roughly equal amounts of rat-specific sequences in a genetically stable recombinant state led to ...1975170369
heterogeneity of surface antigens on endogenous type c virus-producing cell sublines derived from a clonal line of balb/3t3 cells.when viral envelope antigens (veas) and virus-associated cell surface antigens (csas) are used as markers of type c viruses, more heterogeneity of virus populations can be demonstrated than by using host range alone. in the present study, four csas (pc1, x.1, gcsa, and mev-sa1) and three veas (xvea, x1-vea, and sub-gsvea) were used as markers of virus populations present in various sublines of the balb/c embryonic fibroblast balb/3t3 clone a31 line. of four spontaneously transformed sublines, tw ...1975171236
an attenuated mink enteritis virus and its use in a trivalent vaccine: studies on safety and antigenicity. 1977200331
characterization of the stage(s) in the virus replication cycle at which the host-cell specificity of the feline parvovirus subgroup is regulated in canine cells.feline panleukopenia virus (fplv), mink enteritis virus (mev), and canine parvovirus (cpv) are classified as a host-range variants. they show different host-range specificity in vivo and host-cell specificity in vitro. for instance, fplv and mev cannot grow or can grow only inefficiently in canine cell lines such as mdck and the canine fibroma cell line a72. here we have studied the mechanism(s) by which the different cell tropism is mediated in vitro. when fplv or mev was inoculated to a72 cell ...19921322591
antigenic and genomic comparisons of some feline parvovirus subspecies strains.fourteen feline parvovirus (fpv) strains isolated from cats, mink and dogs were comparatively examined on their antigenic and genetic diversities by using monoclonal antibodies against feline panleukopenia virus (fplv) and restriction enzyme analysis of viral dna. mink enteritis virus (mev) strains recently isolated in the northeastern area of the people's republic of china were found to possess more similar antigenic and genetic properties to the antigenic variant virus of canine parvovirus (cp ...19911649516
studies on preparation of inactivated vaccines from cell cultures of mink enteritis virus (mev) and their this paper, superhigh reproductive rate strains of mev with titre more than ha8192x or tcid50 log10(9.7) have been achieved both by cultivation in cell lines with different susceptibility to mev and by isolating and identifying in field by the author. the systematic tests proved that s18 and l12 strains of mev are the best strains for vaccine preparation. in this study, the best means for the tissue cultivation of mev and the most advanced technological process for the production and detectio ...19911666290
response of cultured human airway epithelial cells to x-rays and energetic alpha-particles.radon and its progeny, which emit alpha-particles during decay, may play an important role in inducing human lung cancer. to gain a better understanding of the biological effects of alpha-particles in human lung we studied the response of cultured human airway epithelial cells to x-rays and monoenergetic helium ions. our experimental results indicated that the radiation response of primary cultures was similar to that for airway epithelial cells that were transformed with a plasmid containing an ...19901975612
construction and nucleotide sequence analysis of an infectious dna clone of the autonomous parvovirus, mink enteritis virus.we have cloned the replicative form (rf-) dna of mink enteritis virus (mev), constructed an infectious recombinant plasmid containing mev dna and determined the nucleotide sequence of the cloned mev dna. rf-dnas were detected and infectious virus was generated when the recombinant plasmid containing the entire mev genome was introduced into feline kidney cell cultures. the mev genome was 5094 nucleotides (nt) in length; the 3' end of the virion strand contained a 205 nt palindromic sequence and ...19912016597
[the use of genetic engineering in veterinary medicine with examples from epidemiology, diagnosis and drug production].the results of genetic engineering have reached practical veterinary medicine already. nevertheless there is a great lack of knowledge among those veterinarians who usually do not work with these methods. therefore we want to give an introduction into the advantages and dangers of this technology concerning veterinary medicine. some important analytical methods are explained. related viruses such as wee and eee or canine parvovirus, feline parvovirus and mink enteritis virus, or the related coro ...19902112273
analysis of experimental mink enteritis virus infection in mink: in situ hybridization, serology, and histopathology.strand-specific hybridization probes were used in in situ hybridization studies to localize cells containing mink enteritis virus (mev) virion dna or mev replicative-form dna and mrna. following the experimental mev infection of 3-month-old unvaccinated mink, a significant increase in serum antibodies to mev was detected at postinfection day (pid) 6, 2 days after the onset of fecal shedding of virus. prior to the appearance of virus in feces, viral dna could be detected in the mesenteric lymph n ...19902159543
the murine situs inversus viscerum (iv) gene responsible for visceral asymmetry is linked tightly to the igh-c cluster on chromosome 12.the iv gene controls left-right determination during murine organogenesis. to map this gene, we analyzed backcross progeny produced by mating (c57bl/6j x mev/ty)f1-iv/+heterozygotes to c57bl/6j-iv homozygotes. hybridization of a murine ecotropic virus probe and several homeotic box gene probes coupled with analysis of dominant visible markers enabled us to exclude the iv locus from much of the mouse genome. spurred by a recent report that mapped the iv gene to mouse chromosome 12 which was not e ...19902365357
mink parvoviruses and interferons: in vitro studies.although interferons can inhibit the replication of a number of viruses, little is known about their ability to inhibit parvovirus replication. therefore, in vitro experiments were done to determine if aleutian disease virus and mink enteritis virus, two autonomously replicating mink parvoviruses, induced interferon, were sensitive to the effects of interferon, or inhibited the production of interferon. the results indicated that these parvoviruses neither induced nor were sensitive to the effec ...19862431162
comparison of feline parvovirus subspecific strains using monoclonal antibodies against a feline panleukopenia virus.four monoclonal antibodies (mab) against a feline panleukopenia virus (fplv) tu 1 strain, one of the host range variants of feline parvovirus (fpv), were produced and applied for antigenic analysis of fplv, canine parvovirus (cpv) and mink enteritis virus (mev). all mabs were considered to be directed at epitopes on the virus capsid surface because they neutralized the infectivity and inhibited the hemagglutination (ha) of the homologous virus as well as other fpv strains. they were of the mouse ...19892544758
a mouse linkage testing stock possessing multiple copies of the endogenous ecotropic murine leukemia virus genome.a new linkage testing stock of the laboratory mouse has been constructed. the stock, designated mev (multiple ecotropic provirus), was developed by inbreeding and selection beginning with the cross of strains c58/j and akxd-14. eleven different murine leukemia virus (mulv) proviruses have been fixed in the mev/1ty strain. nine of these can be uniquely identified by southern blotting of pvuii-digested dna and probing with a cloned fragment of the ecotropic viral genome. two proviruses had been ma ...19892571572
characterization of replicative form dna of the autonomous parvovirus mink enteritis virus.characterization of replicative form (rf) dna of mink enteritis virus (mev) was carried out. most of the rf dna were bound to terminal protein but some were free from the protein. the protein-free rf dna increased about 7 times from 30 to 50 hr post-infection, while the dna with protein increased less. the molecules of the replicative intermediate which were partially single-stranded dna and bound to terminal protein were present. two terminal conformations, "extended" and "turnaround," were obs ...19892586345
distribution and movement of membrane-associated platelet glycoproteins: use of colloidal gold with correlative video-enhanced light microscopy, low-voltage high-resolution scanning electron microscopy, and high-voltage transmission electron microscopy.scanning electron microscopy (sem), especially low-voltage (1 kev) high-resolution sem, can be used in conjunction with stereo pair high-voltage (1 mev) transmission electron microscopy (hvem) of whole spread cells or thick sections effectively to correlate surface structure with internal structure. surface features such as microvilli, pits, pseudopodia, ruffles, attached virus, and other surface-related morphologic characteristics can be identified using sem, while underlying cytoskeletal struc ...19892773810
comparisons of feline panleukopenia virus, canine parvovirus, raccoon parvovirus, and mink enteritis virus and their pathogenicity for mink and ferrets.parvoviruses from mink (mink enteritis virus [mev]), cats (feline panleukopenia virus [fpv]), raccoons (raccoon parvovirus [rpv]), and dogs (canine parvovirus [cpv]) were compared. restriction enzyme analysis of the viral replicative-form dna revealed no consistent differences between fpv and rpv isolates, but cpv and mev isolates could be distinguished readily from other virus types. feline panleukopenia virus, rpv, and mev, but not cpv, replicated to high titers in mink. however, on the first ...19872823648
influencing of ionizing radiation on herpes simplex virus and its genome.the effects of cobalt-60 gamma-rays, 10 mev electrons and 52 mev deutrons on the survival of plaque-forming ability has been studied in various strains of herpes simplex virus (hsv). the results show that the d0 for the loss of plaque-forming ability in different hsv strains lies in the range 1-3 kgy. irradiation of isolated hsv-1 dna with cobalt-60 gamma-rays resulted in damage, as indicated by electrophoresis of purified viral dna and by restriction endonuclease analysis, at doses of 1 kgy, wi ...19872824393
[the growth of attenuated strains of canine parvovirus, mink enteritis virus, feline panleukopenia virus, and rabies virus on various types of cell cultures].the growth characteristics were studied in the attenuated strains of canine parvovirus cpva-bn 80/82, mink enteritis virus meva-bn 63/82 and feline panleucopenia virus fpva-bn 110/83 on the stable feline kidney cell line fe, and in the attenuated canine distemper virus cdv-f-bn 10/83 on chicken embryo cell cultures (keb) and cultures of the stable cell line vero. when the fe cultures were infected with different parvoviruses in cell suspension at moi 2-4 tkid50 per cell, the first multiplication ...19872827364
a solid-phase fluorescent immunoassay for detecting canine or mink enteritis parvoviruses in faecal samples.mink enteritis virus (mev) and canine parvovirus (cpv) were detected in faecal samples from experimentally or naturally infected minks and dogs, respectively, using antibody-coated polyacrylamide beads (immunobeads, ib) as the solid phase for immunofluorescence (if) tests. the specificity and sensitivity of the immunobead assay (iba) were studied by comparing it with an enzyme-linked immunoassay (elisa), a haemagglutination (ha) test and an if test using tissue cultures. the iba was as sensitive ...19872830702
characterization of biological and antigenic properties of raccoon dog and blue fox parvoviruses: a monoclonal antibody study.parvovirus isolates from blue foxes and raccoon dogs were characterized by studying their haemagglutination properties, host range in vitro and antigenic structure. in all 3 characters, raccoon dog parvovirus resembled canine parvovirus (cpv), while blue fox parvovirus was similar to mink enteritis virus (mev). monoclonal antibodies (mabs) were prepared against both viruses. raccoon dog parvovirus, while resembling cpv, had a unique antigenic site which could be specified by mabs. the pattern of ...19882836994
dna-mediated gene transfer efficiency is enhanced by ionizing and ultraviolet irradiation of rodent cells in vitro. i. kinetics of enhancement.the enhancement effects of ionizing and ultraviolet radiation on the efficiency of dna-mediated gene transfer were studied. the established cell line, rat-2, consists of cells that are density-dependent contact-inhibited and produce flat monolayers in vitro. when these cells are infected with sv40 virus, a small fraction of cells becomes morphologically "transformed" due to the stable expression of the viral a-gene. rat-2 cells are competent for dna-mediated gene transfer, deficient in thymidine ...19853001817
detection of mink enteritis virus in mink feces, using enzyme-linked immunosorbent assay, hemagglutination, and electron microscopy.twenty-five mink were inoculated with mink enteritis virus (mev). fecal specimens were collected daily and were simultaneously evaluated for mev antigen by use of a direct enzyme-linked immunosorbent assay (elisa), hemagglutination (ha), and electron microscopy. results of the evaluations indicated that mev was shed in the feces on postinoculation days 5 and 6. the virus was not detectable by elisa or ha after postinoculation day 6, although viruses were found in reduced numbers by use of electr ...19863021033
biological and physical comparison of mink enteritis virus feline panleukopenia virus and canine parvovirus. 19863023731
rederivation of mhv and mev antibody positive mice by cross-fostering and use of the microisolator caging system.this study established the feasibility of rederiving numerous mouse hepatitis virus (mhv) and mouse encephalomyelitis virus (mev) antibody positive strains of mice using cross fostering techniques and a new caging system, thus permitting introduction of virus antibody free mice into a barrier facility. serologic status of dams within the nucleus breeding colony was determined, and all mice within the breeding colony were housed in individual microisolator cages. specific pathogen free (spf) fost ...19873037189
the propagation of feline panleukopenia virus in micro-carrier cell culture and use of the inactivated virus in the protection of mink against viral enteritis.using microcarrier cell culture for the production of virus antigen, a formalin-inactivated feline panleukopenia virus vaccine was evaluated for protection of mink against specific mink enteritis virus infection. the vaccine showed a good immunogenic effect in mink when used either alone or in combination with clostridium botulinum type c-toxoid and/or pseudomonas aeruginosa vaccine. a single vaccination induced persistent immune responses for periods of at least 1 year, as evaluated by elisa an ...19873037766
evidence for a multiple innervation of cerebellar purkinje cells by climbing fibers in adult ferrets infected at birth by a mink enteritis virus.newborn ferrets were inoculated with mink enteritis virus (parvovirus). they developed a cerebellar hypoplasia and presented severe ataxia. electrophysiological study by intracellular recordings in the cerebellar cortex demonstrates that in these ferrets, like in other mammals, purkinje cells deprived from granule cell input during development remain multiply innervated by climbing fibers in the adult.19873040186
canine parvovirus: strain difference in haemagglutination activity and antigenicity.canine parvovirus (cpv) strains were compared for haemagglutination (ha) activity and antigenicity and were divided into two types by ha activity. strains cp49 and 29-f showed temperature-dependent ha, like feline panleukopenia virus (fplv) and mink enteritis virus (mev), whereas strains sp-80 and y-1 showed temperature-independent ha with erythrocytes from eight species of animals. the results of a cross ha inhibition test using immunized rat sera suggested that of the two types of cpv those sh ...19883339330
apparent lack of effect of vaccination against mink enteritis virus (mev). a challenge study.the mink enteritis virus part of a triple vaccine was tested in mink. no raise in antibody response was measured after vaccination. subsequent challenge of groups of vaccinated or not-vaccinated animals revealed no differences in virus excretion or antibody response in the different animals.19883369943
some clinical and hematological features of virus enteritis of mink.twenty-six, ten-week-old mink were infected by force feeding by pipette 2 ml of a tissue suspension containing a wisconsin strain of mink enteritis virus. four days later, diarrhea and partial or complete loss of appetite developed simultaneously in all of the animals. squinting and occasional vomiting were also observed. by the sixth day after inoculation, all of the mink were anorectic and weak. anorexia persisted for 48 to 96 hours. diarrhea and vomiting continued until the eighth to ninth da ...19694238566
pathological changes in virus enteritis of mink.the lesions which characterize viral enteritis of mink (vem) were studied in twenty-six, ten-week-old mink which had been infected by force feeding a tissue suspension containing a wisconsin strain of mink enteritis virus. the pathogenesis of the lesions was reconstructed from gross and histopathological changes observed in animals which were selected randomly from the group each day for necropsy during the course of the disease. alterations were observed in the tissues of all mink examined from ...19704246838
preparation of poliovirus labeled with phosphorus-33.phosphorus-33 ((33)p), a weak (0.25 mev) beta-emitting isotope of phosphorus with a half-life of 25 days, has been used to label poliovirus in cell culture. hela cell monolayers were depleted of phosphate and then labeled by incubating at 37 c in a medium (lm) containing about 10 muci of (33)p as orthophosphate per ml. labeled cells were infected at a high multiplicity with poliovirus type 1 and incubated for 8 hr in lm medium. virus from infected cells was then concentrated and purified. virus ...19724336662
studies on mink enteritis virus. 19664963726
feline panleucopaenia virus--in vitro comparison og strains with a mink enteritis virus. 19674965340
characterization of antigenic variation among mink enteritis virus isolates.three antigenic forms of natural field isolates of mink enteritis virus were revealed with a panel of monoclonal antibodies generated against the closely studied feline panleukopenia virus and canine parvovirus-2 virus. two types (types 2 and 3) were shown to be closely related by agar-gel precipitin tests and by restriction enzyme mapping. however, types 2 and 3 differed from the type 1 isolates in the same tests. in cross-protection studies, inactivated viral vaccines made from any one of the ...19846084433
antigenic relationships between canine parvovirus type 2, feline panleukopenia virus and mink enteritis virus using conventional antisera and monoclonal antibodies.the antigenic relationships between three similar parvoviruses, canine parvovirus type 2 (cpv), feline panleukopenia virus (fpv) and mink enteritis virus (mev) were investigated. antisera against all 3 viruses and monoclonal antibodies (mab) to cpv were prepared and the viruses compared using several serological methods. when conventional sera were used in the hemagglutination-inhibition and agar gel precipitin (agp) tests there were no differences between the cpv viral isolates studied, but ant ...19826180709
canine parvovirus: relationship to wild-type and vaccine strains of feline panleukopenia virus and mink enteritis virus.canine parvovirus (cpv), feline panleukopenia virus (fplv) and mink enteritis virus (mev) were compared serologically, by determination of their host range in cell cultures, as well as by restriction enzyme analysis. maps of the virus genomes were established using seven different restriction enzymes cutting at a total of 56 sites. mev and fplv gave maps which were identical except for one restriction site. the map of cpv is closely related to those of fplv/mev since their dnas share about 80% o ...19826181186
antigenic structure and variation of canine parvovirus type-2, feline panleukopenia virus, and mink enteritis virus.the antigenic structure and variation of canine parvovirus type-2 (cpv), feline panleukopenia virus (fpv), mink enteritis virus (mev), and a closely related virus of raccoons (rpv) was investigated using a panel of 13 monoclonal antibodies (mab) formed against cpv and 8 mab formed against fpv. each mab both neutralized and inhibited the hemagglutination of the homologous virus. all mab tested immunoprecipitated the two capsid proteins. five mab were specific for the cpv isolates and one reacted ...19836194613
hemagglutination by canine parvovirus: serologic studies and diagnostic applications.conditions for canine parvoviral hemagglutination (ha) and hemagglutination-inhibition (hi) reactions were defined. the ha phenomena were used to differentiate canine parvovirus (cpv) from feline panleukopenia virus (fpv), mink enteritis virus (mev), and minute virus of canines. serologic comparisons of the cpv, fpv, and mev by ha-hi and serum-neutralization tests indicated that cpv, fpv, and mev were antigenically similar but were different from minute virus of canines. diagnostic application o ...19806250432
comparison of canine parvovirus with mink enteritis virus by restriction site mapping.the genomes of canine parvovirus and mink enteritis virus were compared by restriction enzyme analysis of their replicative-form dnas. of 79 mapped sites, 68, or 86%, were found to be common for both types of dna, indicating that canine parvovirus and mink enteritis virus are closely related viruses. whether they evolved from a common precursor or whether canine parvovirus is derived from mink enteritis virus, however, cannot be deduced from our present data.19816264109
successful experimental challenge of dogs with canine parvovirus-2.withholding food from dogs for 24 hours prior to, and for 48 hours following oral challenge with a gut mucosal homogenate of canine parvovirus-2, was a successful means of reproducing gastroenteric signs of canine parvovirus-2 infection. twenty-one of 24 dogs, which had previously received various vaccine preparations of mink enteritis virus or were unvaccinated, and which were starved at challenge, developed soft or liquid feces with large or without large clots of mucus. altered feces were mos ...19826280819
the failure of an inactivated mink enteritis virus vaccine in four preparations to provide protection to dogs against challenge with canine parvovirus-2.four experimental vaccine preparations comprising a strain of mink enteritis virus inactivated by either formalin or beta-propiolactone, and either adjuvanted or nonadjuvanted, failed to stimulate a consistent serum antibody response in 20 vaccinated dogs and failed to protect all but one of these dogs against oral challenge with canine parvovirus-2.19826280820
amphotericin b selection of mutant chinese hamster cells with defects in the receptor-mediated endocytosis of low density lipoprotein and cholesterol biosynthesis.this paper describes a rapid and efficient two-step procedure for the isolation of mutant cells with defects in receptor-mediated endocytosis. the procedure takes advantage of two fungal metabolites, compactin (ml236b), a potent inhibitor of cholesterol biosynthesis, and amphotericin b, a polyene antibiotic that forms toxic complexes with sterols in membranes. mutagen-treated chinese hamster ovary cells were preincubated overnight in a medium containing mevalonate, low density lipoprotein (ldl), ...19836310583
sera from irradiated rats contain antibodies to a ubiquitous tumour-associated antigen.female rats of the inbred strains bn/birij and wag/rij were irradiated with 30 kv x-rays, or 15 mev or 0.5 mev fast neutrons. sera were collected several months after irradiation and found to be negative for antibodies reacting with the murine mammary tumour virus as tested by a solid-phase radioimmunoassay and an immunofluorescence absorption test. we found, however, that in an immunofluorescence assay several sera from irradiated rats reacted with a cytoplasmic antigen in rat mammary, ureter a ...19836311562
comparison of the viral proteins of canine parvovirus-2, mink enteritis virus and feline panleukopenia virus.canine parvovirus-2 (cpv-2), mink enteritis virus (mev) and feline panleukopenia virus (fpv) were produced using identical cell culture and purification techniques. the distributions of the haemagglutinating activity of the three different parvoviruses in a cscl gradient were similar with haemagglutinating peaks identified at 1.48-1.49, 1.42, 1.36 and 1.30-1.31 g cm-3. the number and distribution of the viral proteins and the equivalent protein molecular weights are similar for all three viruses ...19836316627
polymorphonuclear leukocyte-associated antigenemia in measles: demonstration and indirect immunoperoxidase technique was used for the identification of measles virus (mev) antigen in routinely prepared peripheral blood smears. characteristic brown immunoperoxidase staining was present in the polymorphonuclear (pmn) leukocytes in 25 of 31 children during the exanthematous stage of measles. no peroxidase staining was seen on peripheral blood smears in any of 9 children convalescing from measles or in 9 children with upper respiratory tract infections ( urti ) without exanth ...19846374033
a non-haemagglutinating isolate of mink enteritis virus.a virus was isolated from mink showing clinical and pathological signs of mink enteritis. this virus was identified as mink enteritis virus (mev) from results of serological tests, determination of its density in cscl (1.415 g cm-3), and morphology, including size (20 nm in diameter). the isolate was designated mev-s. in contrast to other known mev strains, the mev-s isolate has no haemagglutinating (ha) activity with swine red blood cells (rbcs) at 4 degrees c and ph 6.8. neither was there any ...19846437057
sensitive detection of canine parvovirus dna by the nested polymerase chain reaction.a polymerase chain reaction (pcr) for the detection of canine parvovirus (cpv) was developed. to increase the sensitivity and specificity of the reaction, the nested pcr with a double-nested primer pair (inner primer pair) was designed. the sequences of the pcr primer pairs were selected from the conserved region in the cpv vp1/vp2 gene. the pcr with the outer or inner primer pair alone (single pcr) could detect 10 fg of viral replicative form (rf) dna on agarose gel electrophoresis; whereas as ...19947801517
[bone sterilization by radiation and the hiv virus].an experimental study was performed to investigate the efficacy of irradiating hiv-contaminated allografts. irradiation was achieved using an accelerator delivering 6.3 mev electrons, and the viral strain was hiv-1/lav-1. at an activity equivalent to 600.000 counts of reverse transcriptase activity per minute and per millilitre, irradiation permitted total inactivation of hiv. in the light of present data concerning plasma viremia in hiv-infected patients, this experiment suggested that irradiat ...19938066295
production of mink enteritis parvovirus empty capsids by expression in a baculovirus vector system: a recombinant vaccine for mink enteritis parvovirus in mink.the vp-2 gene of mink enteritis parvovirus (mev) was amplified by the polymerase chain reaction using mev dna isolated from the faeces of a naturally infected mink. subsequently the vp-2 gene was cloned into a baculovirus expression vector. recombinant baculoviruses were isolated and the mev vp-2 gene product was characterized after expression in sf9 insect cells. the mev vp-2 product had the same size as that reported for the wild-type mev vp-2 protein and was recognized by convalescent sera fr ...19948113722
mapping of determinants of the host range for canine cells in the genome of canine parvovirus using canine parvovirus/mink enteritis virus chimeric viruses.feline panleukopenia virus (fplv), mink enteritis virus (mev) and canine parvovirus (cpv) are more than 98% similar in dna and predicted amino acid sequences, but they show different host-cell specificities; cpv is able to replicate in canine cells in culture, whereas fplv and mev cannot or replicate only to a low titre. to map the genomic region responsible for the host range of cpv in vitro, cpv/mev chimeric viruses were generated by transfecting infectious cpv/mev chimeric plasmids into a cul ...19948207398
two dominant neutralizing antigenic determinants of canine parvovirus are found on the threefold spike of the virus capsid.the 25-nm diameter parvovirus capsid is assembled from 60 copies of a sequence common to the overlapping vp1 and vp2 proteins. here we examine the epitope specificity's of 28 monoclonal antibodies (mab) prepared against canine parvovirus (cpv), feline panleukopenia virus (fpv), and raccoon-dog parvovirus or blue (arctic) fox parvovirus. comparing the reactivity of those mab with various mab-selected escape mutants, or with natural variants of cpv or mink enteritis virus (mev) which differ at kno ...19948259653
the mev mouse linkage testing stock: mapping 30 novel proviral insertions and establishment of an improved stock.continuing efforts to improve the mev linkage testing stock are described. the mev stock carries multiple copies of the ecotropic murine leukemia virus genome. these proviruses are individually detectable in southern blots, making it possible to follow the segregation of many loci simultaneously in genetic crosses. about 50 novel proviral insertions have been detected in this stock and propagated to homozygosity in a number of sublines. many of the proviruses were first observed in individuals t ...19938390965
[nucleotide sequence and genome structure of mink enteritis virus].this paper reports the sequence of the cloned mink enteritis virus (mev) rf dna and its 3' end and 5' end structure predicted. mev genome is near 5064 nucleotides (nt) in length. in its 5' end non-coding region, there are three 51 nt repeated sequences. the nucleotide sequence of mev genome has very high homology with those of canine parvovirus (cpv) and feline parvovirus (fpv), reaching 99.1% and 99.9% respectively in the structure gene region. but they differ notably in 5' end non-coding regio ...19938396953
full protection in mink against mink enteritis virus with new generation canine parvovirus vaccines based on synthetic peptide or recombinant protein.two recently developed vaccine--one based on synthetic peptide and one based on recombinant capsid protein--fully protected dogs against heavy experimental canine parvovirus (cpv) infection. the high sequence homology ( > 98%) and antigenic similarity between cpv and mink enteritis virus (mev), feline panleukopenia virus, and raccoon parvovirus, suggest that both vaccines could protect mink, cats and raccoons against these respective host range variants. this was tested in mink and turned out to ...19958525686
[the value of conventionally fractionated radiotherapy in the local treatment of hiv-related kaposi's sarcoma].during the course of aids, 25 to 44% of homosexual patients infected with the human immunodeficiency virus develop kaposi's sarcoma. main manifestation is the skin. response rates of 80 to 100% can be achieved with total dosage up to 50 gy. nevertheless, remissions can also be attained with 20 gy of fractionated radiotherapy. as clinical data on low dose conventional fractionated radiotherapy are insufficient we analysed the response rates of an overall dose of 20 gy in conventional fractionatio ...19958571179
peptide epitope binding specificity and v k and v h gene usage in a monoclonal igm natural autoantibody to t cell receptor cdr1 from a viable motheaten mouse.autoantibodies (aabs) to t cell receptor (tcr) determinants are produced in humans during the course of rheumatoid arthritis and systemic lupus erythematosus as well as in retroviral infections. we have examined the binding specificity of a panel of monoclonal antibodies derived from mutant viable motheaten (mev) mice against several tcr peptide determinants representing the complementary determining region 1 (cdr1) regions of various vbeta families, and have identified one mab, un37-5, that sho ...19968860695
isolation of canine parvovirus from a cat manifesting clinical signs of feline panleukopenia.twenty-seven feline parvovirus (fpv) isolates were recovered from cats clinically diagnosed with feline panleukopenia (fpl) for assessing antigenic and genomic properties of fpl viruses (fplv) recently prevalent among cats in japan. all isolates, with the exception of one novel isolate, fpv-314, possessed homologous properties, and their subgroups in fpvs were identified as fplv. the fpv-314 isolate, which was from a 1.5-year-old cat which manifested clinical signs of fpl and died on the 13th da ...19968862565
detection of canine parvovirus antigens with antibodies to synthetic peptides.antibodies produced in rabbits against an 18-amino acid peptide (peptide 1, nslpqsegatnfgdigvp) of capsid protein vp2/residues 292-309 of canine parvovirus (cpv) or against an 18-amino acid peptide (peptide 2, gkrntvlfhgpastkgks) of nonstructural protein ns1/residues 391-409 of cpv identified, in immunofluorescence analysis, viral antigens in canine a 72 cells infected with cpv. antibodies to peptide 2 also identified viral antigens in bovine cells infected with bovine parvovirus. in western blo ...19968893795
plant-derived vaccine protects target animals against a viral disease.the successful expression of animal or human virus epitopes on the surface of plant viruses has recently been demonstrated. these chimeric virus particles (cvps) could represent a cost-effective and safe alternative to conventional animal cell-based vaccines. we report the insertion of oligonucleotides coding for a short linear epitope from the vp2 capsid protein of mink enteritis virus (mev) into an infectious cdna clone of cowpea mosaic virus and the successful expression of the epitope on the ...19979062924
epitope mapping of a monoclonal antibody specific to feline panleukopenia virus and mink enteritis obtain monoclonal antibodies (mabs) specific to feline panleukopenia virus (fplv) and mink enteritis virus (mev), 15 hybridomas secreting mabs against mev-abashiri were established and the properties of the mabs were analyzed. the cross-reactivity of mabs revealed that one mab, p2-215 was specific for fplv and mev, whereas the remaining fourteen mabs reacted with canine parvovirus (cpv), fplv, and mev. epitope analyses using various cpv/mev chimeric viruses revealed that the mab p2-215 recogn ...19979070987
evaluation of immune response and protection in animals given mink parvovirus orthogonal and pair design tests, mink, guinea-pigs and rabbits were inoculated with inactivated mink enteritis virus (mev) vaccine prepared with mineral oil or al(oh)3 gel adjuvant. the animals were examined for serum haemagglutination-inhibition (hi) antibody during 6-55 days postvaccination (p.v.) and serum-neutralizing (sn) antibody for 11-31 days p.v. differences between mineral oil and al(oh)3 gel adjuvant vaccines in the induction of hi and sn antibodies during 6-55 days p.v. were not ...19979167013
studies on isolation, serum-free cultivation and manufacture of mink enteritis virus optimized for vaccine preparation.optimum conditions have been determined for growth of mink enteritis virus (mev) in the feline kidney cell (fkc) lines. the s18 and s36 strains of mev grow to titres of 8192 to 32,786 haemagglutination (ha) units or 10(-9.7-10.3) tcid50 by alternate cultivation for 22 in fkc with very different susceptibility. mink with typical clinical symptoms of mink viral enteritis (mve) eliminated intestinal mucosal cylinders from which the l12 strain of mev was isolated, which is capable of replication to ...19979167014
two parvoviruses that cause different diseases in mink have different transcription patterns: transcription analysis of mink enteritis virus and aleutian mink disease parvovirus in the same cell line.the two parvoviruses of mink cause very different diseases. mink enteritis virus (mev) is associated with rapid, high-level viral replication and acute disease. in contrast, infection with aleutian mink disease parvovirus (adv) is associated with persistent, low-level viral replication and chronic severe immune dysregulation. in the present report, we have compared viral transcription in synchronized crfk cells infected with either mev or adv using a nonradioactive rnase protection assay. the ov ...19979188563
inactivation of viruses by beta-propiolactone in human cryo poor plasma and igg concentrates.virus inactivation by cold treatment with beta-propiolactone (bpl) was investigated in human cryo poor plasma and purified igg concentrates spiked with relevant human viruses or appropriate animal model viruses. the samples were treated with 0.1 or 0.25% bpl for 300 or 480 min, respectively. residual infectivity was determined by standard microtitration assays on tissue culture cells. the inactivation of all viruses tested was more effective in igg than in plasma. igg: r1=4-5.5 log10 for vesicul ...19989811521
[influence of irradiation on the risk of hiv virus transmission by bone allograft].the purpose of this study was to determine, when assuming the worst case scenario of bone contamination, the sterility assurance level in bone transplantation treated by irradiation and after donor screening.19989846322
the genome nucleotide sequence of a contemporary wild strain of measles virus and its comparison with the classical edmonston strain genome.the only complete genome nucleotide sequences of measles virus (mev) reported to date have been for the edmonston (ed) strain and derivatives, which were isolated decades ago, passaged extensively under laboratory conditions, and appeared to be nonpathogenic. partial sequencing of many other strains has identified >/=15 genotypes. most recent isolates, including those typically pathogenic, belong to genotypes distinct from the edmonston type. therefore, the sequence of ed and related strains may ...199910191199
the influence of prematurity and low birthweight on transplacental antibody transfer in sri lanka.the influence of gestational age, the neonate's birthweight, and maternal age, weight, height and parity on transplacental antibody transfer was assessed in 141 mothers from sri lanka and their neonates. paired blood samples were collected from the mothers and the umbilical cords of the newborns. the sera separated from these samples were categorized as: preterm but adequate birthweight (< 37 weeks' gestation and birthweight > or = 2500 g); term but low birthweight (> or = 37 weeks' gestation an ...199910474642
molecular and structural basis of the evolution of parvovirus tropism.parvoviruses have small genomes and, consequently, are highly dependent on their host for various functions in their reproduction. since these viruses generally use ubiquitous receptors, restrictions are usually intracellularly regulated. a lack of mitosis, and hence absence of enzymes required for dna replication, is a powerful block of virus infection. allotropic determinants have been identified for several parvoviruses: porcine parvovirus, canine parvovirus (cpv), feline parvovirus (feline p ...199910497831
genetic characterization of feline parvovirus sequences from various carnivores.infections with viruses of the feline parvovirus subgroup such as feline panleukopenia virus (fpv), mink enteritis virus (mev) and canine parvovirus (cpv-2) [together with its new antigenic types (cpv-2a, cpv-2b)] have been reported from several wild carnivore species. to examine the susceptibility of different species to the various parvoviruses and their antigenic types, samples from wild carnivores with acute parvovirus infections were collected. viral dna was amplified, and subsequently anal ...200010644832
recovery of pathogenic measles virus from cloned cdna.reverse genetics technology so far established for measles virus (mev) is based on the edmonston strain, which was isolated several decades ago, has been passaged in nonlymphoid cell lines, and is no longer pathogenic in monkey models. on the other hand, mevs isolated and passaged in the epstein-barr virus-transformed marmoset b-lymphoblastoid cell line b95a would retain their original pathogenicity (f. kobune et al., j. virol. 64:700-705, 1990). here we have developed mev reverse genetics syste ...200010864679
the influence of prematurity and low birthweight on transplacental antibody transfer in a rural west african determine the influence of prematurity and low birthweight (lbw) on transplacental antibody transfer.200111469946
caspase activation is required for permissive replication of aleutian mink disease parvovirus in vitro.aleutian mink disease parvovirus (adv) is distinct among the parvoviruses as infection in vivo is persistent, restricted, and noncytopathic. in contrast, infections with other more prototypic parvoviruses, like mink enteritis virus (mev), are acute, cytopathic, and characterized by permissive replication in vivo. although apoptosis results in the death of cells acutely infected by parvoviruses, the role of apoptosis in adv infections is unknown. permissive infection of adv resulted in apoptosis ...200211878925
recognition of the measles virus nucleocapsid as a mechanism of irf-3 activation.the mechanisms of cellular recognition for virus infection remain poorly understood despite the wealth of information regarding the signaling events and transcriptional responses that ensue. host cells respond to viral infection through the activation of multiple signaling cascades, including the activation of nf-kappab, c-jun/atf-2 (ap-1), and the interferon regulatory factors (irfs). although viral products such as double-stranded rna (dsrna) and the processes of viral binding and fusion have ...200211907205
[newly isolated mink parvovirus strain].a new mink enteritis virus (mev) strain, called cherepanovo, was isolated in the novosibirsk region (west siberia). the level of this strain's accumulation in cell culture is lower than that of commercial mev strains rodniki or beregovoi. identification of cherepanovo strain by hemagglutination inhibition test, protein electrophoresis in denaturing polyacrylamide gel, and pcr showed its similarity to the previously described mev strains. comparative analysis of this strain's 5' and 3' nucleotide ...200212046466
measles virus suppresses interferon-alpha signaling pathway: suppression of jak1 phosphorylation and association of viral accessory proteins, c and v, with interferon-alpha receptor establish infections, viruses use various strategies to suppress the host defense mechanism, such as interferon (ifn)-induced antiviral state. we found that cells infected with a wild strain of measles virus (mev) displayed nearly complete suppression of ifn-alpha-induced antiviral state, but not ifn-gamma-induced state. this phenomenon is due to the suppression of ifn-alpha-inducible gene expression at a transcriptional level. in the ifn-alpha signal transduction pathway, jak1 phosphorylatio ...200312620806
epizootiology on mink enteritis. ii. musca domestica l as a possible vector of virus.the significance of the house fly m. domestica l as a vector of me was studied. mev was transmitted from infected to susceptible mink by flies having unlimited contact with both. disease was also induced by feeding infected flies to susceptible mink.196514290947
epizootiology of mink enteritis: i. stability of the virus in feces exposed to natural environmental factors.mev infective feces, half-buried in the ground for 9 months, (from november to july), contained infectious virus when inoculated into susceptible hosts. it is believed that mev in feces, if protected by favorable environmental conditions, is capable of infecting mink for an indefinite period beyond nine months.196514294805
epizootiology of mink enteritis: 3. carrier state in mink.the mev carrier state in mink was studied and evidence that mink may carry the virus and excreted it in feces over a period of a year is presented. the significance of the carrier state is discussed especially in regard to the epizootiology of the disease.196514300859
immunodominant domains of the measles virus hemagglutinin protein eliciting a neutralizing human b cell response.the most important neutralizing and protective antibodies against measles virus (mev) are directed against the hemagglutinin protein (mev-h). to define the mev binding domains recognized by human antibodies a set of 10 non-redundant mev-h-specific monoclonal antibodies (mabs) was used to block their binding in a competition elisa. sera from both naturally infected and vaccinated individuals showed similar competition patterns. two distinct domains were identified as the main target of human anti ...200314579178
growth arrest of epithelial cells during measles virus infection is caused by upregulation of interferon regulatory factor 1.natural infection with measles virus (mev) is initiated when the virus reaches epithelial cells in the respiratory tract, oropharynx, or conjunctivae. human epithelial cells infected with mev frequently show growth suppression. in this study, we investigated the possible mechanisms for this suppression. the bronchiolar epithelial cell a549 showed growth arrest in g(0)/g(1) following mev infection or treatment with gamma interferon (ifn-gamma). ifn regulatory factor-1 (irf-1) was upregulated duri ...200415078941
improved antiviral activity in vitro of ribavirin against measles virus after complexation with cyclodextrins.despite vaccination, measles remains a burden in both developed and developing countries and complications may necessitate an efficient therapy. measles virus (mev) is susceptible to ribavirin (rbv), but the use of this drug is limited by its toxicity. cyclodextrins (cds) can form complexes with numerous molecules, improving their bioavailability and their biological properties. we have evaluated in vitro the antiviral effects of complexes of rbv with alpha-, beta- or gamma-cd against two clade ...200415130537
influence of irradiation on the risk of transmission of hiv in bone grafts obtained from appropriately screened donors and followed by radiation sterilization.we studied the effects of radiation (electrons of 6.2 mev) at different temperatures with respect to the inactivation of the human immunodeficiency virus to determine the radiosensitivity of the virus. using a mathematical model describing the dependence on radiation dose of the proportion of sterile items in a population of bone allografts contaminated by hiv, and subjected to irradiation, we have commented on and explained the calculation of the sterility assurance level in bone transplantatio ...200015256937
efficient rescue of measles virus from cloned cdna using slam-expressing chinese hamster ovary cells.we here report a highly efficient reverse genetics system for measles virus (mev), using chinese hamster ovary cells constitutively expressing a mev receptor human signaling lymphocyte activation molecule (cho/hslam cells). the recombinant vaccinia virus vtf7-3 that encodes the t7 rna polymerase under the control of the early/late promoter was used in the system. replication of vtf7-3 was highly restricted in cho/hslam cells, but the virus could still drive the t7 promoter, allowing us to recove ...200515681066
effects of lyophilization on the infectivity of enveloped and non-enveloped viruses in bone tissue.recently reported qualitative experiments proved that retroviral infectivity is not destroyed by lyophilization performed on systemically infected bone and tendon. the now accomplished quantitative determination of residual infectivity for enveloped and non-enveloped viruses allows a validation of the production process regarding viral safety in freeze-dried bone transplants. the lyophilization effect on the infectivity of two non-enveloped viruses (maus elberfeld virus, mev; porcine parvovirus, ...200515946737
two mink parvoviruses use different cellular receptors for entry into crfk cells.mink enteritis virus (mev) and aleutian mink disease parvovirus (adv) are two mink parvoviruses that replicate permissively in crandell feline kidney (crfk) cells. we have used this cell model to examine if these two mink parvoviruses use the same cellular receptor. whereas the cellular receptor for mev is expected to be the transferrin receptor (tfr), the cellular receptor for adv has not been clearly identified. we used short hairpin rnas (shrnas) produced from plasmids to trigger rna interfer ...200516040076
long untranslated regions of the measles virus m and f genes control virus replication and cytopathogenicity.measles is still a major cause of mortality mainly in developing countries. the causative agent, measles virus (mev), is an enveloped virus having a nonsegmented negative-sense rna genome, and belongs to the genus morbillivirus of the family paramyxoviridae. one feature of the moribillivirus genomes is that the m and f genes have long untranslated regions (utrs). the m and f mrnas of mev have 426-nucleotide-long 3' and 583-nucleotide-long 5' utrs, respectively. though these long utrs occupy as m ...200516254369
inhibition of measles virus and subacute sclerosing panencephalitis virus by rna interference.subacute sclerosing panencephalitis (sspe) is a rare, but fatal outcome of measles virus (mev) infection. sspe develops after prolonged persistence of mutated mev called sspe virus. although a combination therapy using interferon and inosiplex or ribavirin appears to prolong survival time to some extent, there is currently no effective treatment to completely cure sspe and a new treatment strategy is greatly needed. in this study, we adopted rna interference (rnai) strategy and examined whether ...200616530274
cell cycle arrest and apoptosis in caenorhabditis elegans germline cells following heavy-ion microbeam investigate positional effects of radiation with an energetic heavy-ion microbeam on germline cells using an experimental model metazoan caenorhabditis elegans.200616546901
generation of measles virus with a segmented rna genome.viruses classified in the order mononegavirales have a single nonsegmented rna molecule as the genome and employ similar strategies for genome replication and gene expression. infectious particles of measles virus (mev), a member of the family paramyxoviridae in the order mononegavirales, with two or three rna genome segments (2 seg- or 3 seg-mev) were generated using a highly efficient reverse genetics system. all rna segments of the viruses were designed to have authentic 3' and 5' self-comple ...200616611883
physical map of the dna genome of autographa californica nuclear polyhedrosis virus.a physical map of the 88 x 10(6) dalton, circular dna genome of autographa californica nuclear polyhedrosis virus was constructed. the complete order of bamhi and xmai restriction enzyme sites was determined. the ecori and hindiii fragments were partially ordered, and their general locations, relative to the bamhi and xmai maps, were determined. alterations in the restriction endonuclease fragment patterns of natural genotypic variants of a. californica nuclear polyhedrosis virus, including tric ...197916789175
molecular detection of measles virus in primary cell cultures of otosclerotic tissue.primary cell cultures were established from otosclerotic/otospongiotic footplate bone particles. although this procedure is time-consuming, the quality and quantity of rna isolated from these cells were much higher in comparison with the direct isolation of rna from footplate bone samples and the preparation was more suitable for the detection of measles virus (mev) rna.200616846922
full-length sequence analysis of subacute sclerosing panencephalitis (sspe) virus, a mutant of measles virus, isolated from brain tissues of a patient shortly after onset of sspe.subacute sclerosing panencephalitis (sspe) virus, a measles virus (mev) mutant, was isolated from brain tissues of a patient shortly after the clinical onset, and the entire viral genome was sequenced. the virus, named sspe-kobe-1, formed syncytia on b95a and vero/slam cells without producing cell-free infectious virus particles, which is characteristic of sspe virus. phylogenetic analysis classified sspe-kobe-1 into genotype d3. when compared with an mev field isolate of the same genotype (ich- ...200616858143
difference in production of infectious wild-type measles and vaccine viruses in monocyte-derived dendritic cells.macrophages (mø) and dendritic cells (dc) are thought to be targets of measles virus (mev) at the early stage of infection. we compared the growth of edmonston-derived vaccine strains and fresh clinical isolates of mev in monocytes, monocyte-derived granulocyte-macrophage colony-stimulating factor (gm-csf)-induced mø (gm-mø) and in monocyte-derived dc (mo-dc). neither vaccine strains nor fresh isolates thrived in monocytes and gm-mø and no differences were evident among them. on the other hand, ...200716959355
neutralizing antibodies against feline parvoviruses in nondomestic felids inoculated with commercial inactivated polyvalent vaccines.the virus neutralization (vn) antibody titers of serum samples from 18 individuals representing 8 carnivore species vaccinated with commercial polyvalent vaccines optimized for domestic cats containing inactivated feline panleukopenia virus (fplv) were evaluated against canine parvovirus type 2 (cpv2). in addition, the titers among 5 individuals from 4 carnivore were evaluated against antigenic variants of feline parvoviruses; fplv, cpv2, cpv2a, cpv2b, cpv2c, mink enteritis virus type 1 (mev1) a ...200617146178
measles virus and otosclerosis.measles virus (mev) might play an important role as an environmental stimulus in the etiopathogenesis of otosclerosis. chronic inflammation was shown in morphologic investigations of otosclerotic foci and mev n, p, and f proteins were detected within cells of the otosclerotic focus by immunohistochemical investigations. mev rna was extracted from fresh-frozen otosclerotic tissue by the use of in vitro rt-pcr. this result was validated through amplification of mev genome sequences by rt-pcr from ...200717245028
establishment of a mink enteritis vaccine production process in stirred-tank reactor and wave bioreactor microcarrier culture in 1-10 l scale.a scale-up and process optimization scheme for the growth of adherent embryonic feline lung fibroblasts (e-fl) on microcarriers and the propagation of a mink enteritis virus (mev) strain for the production of an inactivated vaccine is shown. stirred-tank cultivations are compared with results obtained from wave bioreactors. transfer from a roller bottle-based production process into large-scale microcarrier culture with starting concentrations of 2g/l cytodex 1 microcarriers and 2.0 x 10(5)cells ...200717391818
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