| [construction of transgenic tobacco expressing tomato ggps2 gene and analysis of its low light tolerance]. | to explore the influence of low light on the synthesis of carotenoids, chlorophyll and the adaptability of transgenic plants with tomato solanum lycopersicon l. ggps2 gene, we constructed a vector containing a ggps2 gene with green fluorescent protein (gfp) as report gene under the control of a cauliflower mosaic virus 35s promoter and introduced it into tobacco nicotiana tabacum l. cv. wisconsin 38 by agrobacterium tumefaciens-mediated transformation. pcr analysis of the dna from kanamycin resi ... | 2015 | 26571690 |
| a novel reference plasmid for the qualitative detection of genetically modified rice in food and feed. | rice is one of the most important food crops in the world. genetically modified (gm) technology has been used in rice to confer herbicide tolerance and pathogen or insect resistance. china invests heavily in research on gm rice. by the end of 2014, at least 250 transgenic rice lines had been developed in china. to monitor the presence of gm rice in food and feed, we collected information on foreign elements from 250 transgenic rice lines and found 5 elements, including the agrobacterium tumefaci ... | 2015 | 26495318 |
| an improved cucumber mosaic virus-based vector for efficient decoying of plant micrornas. | we previously devised a cucumber mosaic virus (cmv)-based vector system carrying microrna target mimic sequences for analysis of microrna function in arabidopsis thaliana. we describe an improved version in which target mimic cloning is achieved by annealing two partly-overlapping complementary dna oligonucleotides for insertion into an infectious clone of cmv rna3 (ls strain) fused to the cauliflower mosaic virus-derived 35s promoter. ls-cmv variants carrying mimic sequences were generated by c ... | 2015 | 26278008 |
| new multiplex pcr methods for rapid screening of genetically modified organisms in foods. | we present novel multiplex pcr methods for rapid and reliable screening of genetically modified organisms (gmos). new designed pcr primers targeting four frequently used gmo specific sequences permitted identification of new dna markers, in particular 141 bp fragment of cauliflower mosaic virus (camv) 35s promoter, 224 bp fragment of agrobacterium tumefaciens nopaline synthase (nos) terminator, 256 bp fragment of 5-enolppyruvylshikimate-phosphate synthase (epsps) gene and 258 bp fragment of cry1 ... | 2015 | 26257724 |
| transgene expression in tick cells using agrobacterium tumefaciens. | ticks transmit infectious agents to humans and other animals. genetic manipulation of vectors like ticks could enhance the development of alternative disease control strategies. transgene expression using the phytopathogen agrobacterium tumefaciens has been shown to promote the genetic modification of non-plant cells. in the present work we developed t-dna constructs for a. tumefaciens to mediate transgene expression in hela cells as well as rhipicephalus microplus tick cells. translational fusi ... | 2015 | 26188857 |
| a simple and efficient genetic transformation method of ganoderma weberianum. | in this study, the agrobacterium tumefaciens-mediated transformation method for ganderma weberianum has been established. driven by the cauliflower mosaic virus (camv) 35s promoter, the hygromycin phosphotransferase (hpt), β-glucuronidase (uida), and enhanced green fluorescent protein (egfp) genes have been efficiently expressed in transgenic mycelia and spores. the transformation system was composed of the growing mycelia, a. tumefaciens strain gv3101, and the expression vector pbi-h1, harborin ... | 2015 | 25655754 |
| simple screening strategy with only water bath needed for the identification of insect-resistant genetically modified rice. | an informative, with simple instrument needed, rapid and easily updated strategy for the identification of insect-resistant genetically modified (gm) rice has been described. such strategy is based on a parallel series of loop-mediated isothermal amplification (lamp) reactions targeting the rice endogenous gene sucrose phosphate synthase (sps), the top two most frequently used genetic elements (agrobacterium tumefaciens nopaline synthase terminator (nos) and cauliflower mosaic virus 35s promoter ... | 2015 | 25582220 |
| cloning and expression of tnf related apoptosis inducing ligand in nicotiana tabacum. | molecular farming has been considered as a secure and economical approach for production of biopharmaceuticals. human tnf related apoptosis inducing ligand (trail) as a promising biopharmaceutical candidate has been produced in different expression hosts. however, little attention has been paid to molecular farming of the trail in spite of numerous advantages of plant expression systems. therefore, in this study the cytoplasmic production of the trail was tackled in nicotiana tabacum using agrob ... | 2015 | 25561925 |
| an efficient plant regeneration and agrobacterium-mediated genetic transformation of tagetes erecta. | tagetes erecta, l. an asteraceous plant of industrial and medicinal value, contains important compounds like pyrethrins, thiophenes and lutein, possessing immense potential for insecticidal, nematicidal and nutraceutical activities. considering the importance and demand for these natural compounds, genetic manipulation of this crop for better productivity of secondary metabolites holds great significance. a rapid and reproducible direct regeneration and genetic transformation system is the prere ... | 2015 | 25504508 |
| development and validation of a p-35s, t-nos, t-35s and p-fmv tetraplex real-time pcr screening method to detect regulatory genes of genetically modified organisms in food. | in routine analysis screening methods based on real-time pcr (polymerase chain reaction) are most commonly used for the detection of genetically modified (gm) plant material in food and feed. screening tests are based on sequences frequently used for gm development, allowing the detection of a large number of gmos (genetically modified organisms). here, we describe the development and validation of a tetraplex real-time pcr screening assay comprising detection systems for the regulatory genes ca ... | 2014 | 25437161 |
| characterization of the promoter of grapevine vein clearing virus. | grapevine vein clearing virus (gvcv) is a recently discovered dna virus in grapevine that is closely associated with the grapevine vein clearing syndrome observed in vineyards in missouri and surrounding states. the genome sequence of gvcv indicates that it belongs to the genus badnavirus in the family caulimoviridae. to identify the gvcv promoter, we cloned portions of the gvcv large intergenic region in front of a gfp gene present in an agrobacterium tumefaciens binary vector. gfp expression w ... | 2015 | 25281563 |
| sri lankan cassava mosaic virus replication associated protein (rep) triggers transposition of is426 in agrobacterium. | we report a high rate of is426 transposition in agrobacterium tumefaciens in the presence of the sri lankan cassava mosaic virus (slcmv) replication associated protein gene (rep). upon conjugal transfer of the binary plasmid pcam-slcmv-rep with the slcmv rep gene in the sense orientation under the transcriptional control of the cauliflower mosaic virus (camv) 35s promoter into the a. tumefaciens vir helper strain eha105, the binary plasmid size increased in all 15 transconjugants studied. southe ... | 2014 | 25135797 |
| detection of genetically modified tomato using pcr coupled with muparaflo microfluidics microarrays. | genetically modified (gm) tomatoes have been approved for commercialization in many countries since the first gm tomato flavr savr was permitted for planting in 1994. to meet the requirement of the gm tomatoes labeling policy, in this study we firstly set up the conventional pcr and multiplex pcr detection system for screening the universal elements transformed into tomato, such as cauliflower mosaic virus 35s (camv 35s) promoter, nopaline synthase (nos) terminator of agrobacterium tumefaciens, ... | 2013 | 24266223 |
| [analytical methods for control of foodstuffs made from bioengineered plants]. | foodstuffs made by modern biotechnology are requiring for special control. analytical methods used for these purposes are being constantly perfected. when choosing a strategy for the analysis, several factors have to be assessed: specificity, sensitivity, practically of the method and time efficiency. to date, the gmo testing methods are mainly based on the inserted dna sequences and newly produced proteins in gmos. protein detection methods are based mainly on elisa. the specific detection of a ... | 2013 | 24006752 |
| stable transformation of ferns using spores as targets: pteris vittata and ceratopteris thalictroides. | ferns (pteridophyta) are very important members of the plant kingdom that lag behind other taxa with regards to our understanding of their genetics, genomics, and molecular biology. we report here, to our knowledge, the first instance of stable transformation of fern with recovery of transgenic sporophytes. spores of the arsenic hyperaccumulating fern pteris vittata and tetraploid 'c-fern express' (ceratopteris thalictroides) were stably transformed by agrobacterium tumefaciens with constructs c ... | 2013 | 23933990 |
| regeneration and agrobacterium-mediated transformation of the apomictic species eulaliopsis binata. | protocols for regeneration and agrobacterium-mediated transformation of the apomictic species eulaliopsis binata were developed. initially, seeds of four genotypes of e. binata were incubated on a callus induction murashige and skoog (ms) basal medium supplemented with three concentrations of 2,4-dichlorophenoxyacetic acid (2,4-d). it was found that 36.2 % of explants developed highly friable callus on medium containing 3.0 mg l(-1) 2,4-d. based on frequency of callus induction, the genotype nei ... | 2013 | 23873641 |
| agroinoculation of a full-length cdna clone of cotton leafroll dwarf virus (clrdv) results in systemic infection in cotton and the model plant nicotiana benthamiana. | cotton blue disease is the most important viral disease of cotton in the southern part of south america. its etiological agent, cotton leafroll dwarf virus (clrdv), is specifically transmitted to host plants by the aphid vector (aphis gossypii) and any attempt to perform mechanical inoculations of this virus into its host has failed. this limitation has held back the study of this virus and the disease it causes. in this study, a full-length cdna of clrdv was constructed and expressed in vivo un ... | 2013 | 23623981 |
| agroinoculation of beet necrotic yellow vein virus cdna clones results in plant systemic infection and efficient polymyxa betae transmission. | agroinoculation is a quick and easy method for the infection of plants with viruses. this method involves the infiltration of tissue with a suspension of agrobacterium tumefaciens carrying binary plasmids harbouring full-length cdna copies of viral genome components. when transferred into host cells, transcription of the cdna produces rna copies of the viral genome that initiate infection. we produced full-length cdna corresponding to beet necrotic yellow vein virus (bnyvv) rnas and derived repl ... | 2013 | 23384276 |
| predictive models for the accumulation of a fluorescent marker protein in tobacco leaves according to the promoter/5'utr combination. | the promoter and 5'-untranslated region (5'utr) play a key role in determining the efficiency of recombinant protein expression in plants. comparative experiments are used to identify suitable elements but these are usually tested in transgenic plants or in transformed protoplasts/suspension cells, so their relevance in whole-plant transient expression systems is unclear given the greater heterogeneity in expression levels among different leaves. furthermore, little is known about the impact of ... | 2013 | 22948957 |
| agroinoculation of the cloned infectious cdnas of lettuce chlorosis virus results in systemic plant infection and production of whitefly transmissible virions. | lettuce chlorosis virus (lcv) is a single stranded, positive strand rna virus that is solely transmitted by specific whitefly vectors (bemisia tabaci biotypes a and b) but not by mechanical leaf-rub inoculation. the roles of viral encoded proteins involved in the infection cycle of lcv have not yet been characterized due to the lack of reverse genetic tools. we present here a report of the successful development of an agrobacterium-mediated inoculation system for the cloned cdna constructs of lc ... | 2012 | 22926259 |
| brachypodium distachyon promoters as efficient building blocks for transgenic research in maize. | the biotechnological approach to improve performance or yield of crops or for engineering metabolic pathways requires the expression of a number of transgenes, each with a specific promoter to avoid induction of silencing mechanisms. in maize (zea mays), used as a model for cereals, an efficient agrobacterium tumefaciens-mediated transformation system has been established that is applied for translational research. in the current transformation vectors, the promoters of the 35s gene of the cauli ... | 2012 | 22523343 |
| multiplex, construct-specific, and real-time pcr-based analytical methods for bt rice with cry1ac gene. | qualitative and quantitative analytical methods based on pcr for bacillus thuringiensis (bt) rice hybrid, namely, mrp 5401 bt expressing a modified version of the bt cry1ac gene, are reported here. multiplex pcr assays were developed to target the cry1ac transgene, cauliflower mosaic virus (camv) 35s promoter, agrobacterium tumefaciens nopaline synthase (nos) terminator, the neomycin phosphotransferase ii (nptll) marker gene, and an endogenous a-tubulin (tuba) gene in bt rice. the 3.178 kb regio ... | 2015 | 22468358 |
| agrobacterium-mediated transformation and regeneration of fertile transgenic plants of chinese cabbage (brassica campestris ssp. pekinensis cv. 'spring flavor'). | a procedure for the regeneration of fertile transgenic chinese cabbage (brassica campestris ssp. pekinensis cv. 'spring flavor') is presented in this report. the protocol is based on infection of cotyledon explants of 5-d-old seedlings with an agrobacterium tumefaciens strain lba4404 carrying a disarmed binary vector ptok/bks-1. the t-dna region of this binary vector contains the nopaline synthase/neomycin phosphotransferase ii (nptii) chimeric gene for kanamycin resistance and the cauliflower m ... | 1995 | 24194308 |
| agrobacterium-mediated transformation of commercial melon (cucumis melo l., cv. amarillo oro). | cotyledon explants of muskmelon (cucumis melo l., cv. amarillo oro) seedlings were co-cultivated with disarmed agrobacterium tumefaciens strain lba4404 that contained the binary vector plasmid pbi121.1. the t-dna region of this binary vector contains the nopaline synthase/neomycin phosphotransferase ii (nptii) chimeric gene for kanamycin resistance and the cauliflower mosaic virus 35s/β-glucuronidase (gus) chimeric gene. after infection, the cotyledon pieces were placed in induction medium conta ... | 1994 | 24193640 |
| expression of soybean-embryo lipoxygenase 2 in transgenic tobacco tissue. | to assess the role of lipoxygenase (lox; ec 1.13.11.12) in plants, we increased the expression of lox in the tissues of nicotiana tabacum l. cv. 'ky 14' by over-expression of the lox2 gene from the soybean (glycine max (l.) merrill) embryo. the lox2 cdna was manipulated by replacing its 5'-untranslated sequence with the translational enhancer of the alfalfa mosaic virus (amv), and subcloned into a plant expression vector, 3' to a duplicated cauliflower mosaic virus 35s promoter. the amv-lox2 con ... | 1992 | 24178044 |
| coat protein mediated resistance to plum pox virus in nicotiana clevelandii and n. benthamiana. | transgenic nicotiana benthamiana and n. clevelandii plants expressing the coat protein of plum pox virus under the control of the 35s promoter from cauliflower mosaic virus were engineered by agrobacterium tumefaciens mediated transformation. the phenomenon of virus resistance was observed at different levels when transgenic plants, expressing the coat protein and control plants were compared after challenge infection with plum pox virus. n. clevelandii coat protein transgenic plants circumvent ... | 1992 | 24213033 |
| introduction and differential use of various promoters in pollen grains of nicotiana glutinosa and lilium longiflorum. | as part of our research to develop an alternative system for the transformation of recalcitrant plant species we investigated the use of the male gametophyte as a transformation vector. therefore the activity of four different promoters (camv 35s, lat52, chia pa2 and tr2') was analyzed in pollen of a dicot (nicotiana glutinosa) and a monocot (lilium longiflorum) plant species. gene constructs in which the ß-glucuronidase (gus) gene was placed under the control of these promoters were introduced ... | 1992 | 24213031 |
| biosynthesis, processing and targeting of the g-protein of vesicular stomatitis virus in tobacco protoplasts. | leaf protoplasts of tobacco (nicotlana tabacum l.) were employed for transfection of chimeric transcriptional gene fusions comprising the 35s promoter from cauliflower mosaic virus, the coding sequence of the g-protein from vesicular stomatitis virus (vsvg) and the transcriptional terminator from the agrobacterium tumefaciens nopaline-synthetase gene. transient expression of the chimeric gene was monitored through northern analysis of total protoplast rna using a labeled vsv cdna probe, and thro ... | 1992 | 24186728 |
| genetic transformation of strawberry by agrobacterium tumefaciens using a leaf disk regeneration system. | an efficient genetic transformation protocol has been developed for strawberry cv. redcoat using agrobacterium tumefadens. the protocol relies on a high frequency (84%) shoot regeneration system from leaf disks. the leaf disks were inoculated with a non-oncogenic agrobacterium tumefadens strain mp90 carrying a binary vector plasmid pbi121 which contains a chimeric nopaline synthase (nos) promoter driven neomycin phosphotransferase (npt ii) gene and a cauliflower mosaic virus 35s (camv35s) promot ... | 1990 | 24226936 |
| detection of bacterial chitinase activity in transformed plant tumour cells using a specific exochitinase substrate. | methods for the detection of bacterial chitinase activity were compared. the soluble substrate p-nitrophenyl-ß-d-n,n diacetyl chitobiose (ndc) was more sensitive in detecting purified chitinase of serratia marcescens than assays measuring degradation of a solid chitin substrate by either radiochemical or colorimetric means. a chimaeric gene containing a s. marcescens chitinase gene under control of a cauliflower mosaic virus 35s promoter and nopaline synthase terminator sequences was constructed ... | 1990 | 24232928 |
| analysis of a chimeric class-i patatin-gus gene in transgenic potato plants: high-level expression in tubers and sucrose-inducible expression in cultured leaf and stem explants. | patatin is a family of lipid acyl hydrolases that accounts for 30 to 40% of the total soluble protein in potato (solanum tuberosum l.) tubers. to examine the regulation of the patatin genes, we constructed a chimeric gene containing 2.5 kb of 5' flanking sequence from the class i patatin genomic clone ps20 transcriptionally fused to β-glucuronidase (gus) and introduced it into potato plants using an agrobacterium tumefaciens tiplasmid vector. while the chimeric gene was expressed at high levels ... | 1989 | 24272716 |
| the 5' flanking region of a barley b hordein gene controls tissue and developmental specific cat expression in tobacco plants. | the 549 base pairs of the 5' flanking region of a barley seed storage protein (b1 hordein) gene were linked to the reporter gene encoding chloramphenicol acetyl transferase (cat). the chimaeric gene was transferred into tobacco plants using agrobacterium tumefaciens. cat enzyme activity was detected in the seeds, but not in the leaves, of the transgenic plants. furthermore, enzyme activity was found only in the endosperm, and only from fifteen days after pollination. in contrast, the constitutiv ... | 1988 | 24277567 |
| introduction of foreign genes into potato cultivars bintje and désirée using an agrobacterium tumefaciens binary vector. | tuber discs of solanum tuberosum cv bintje and désirée were cocultivated with an agrobacterium tumefaciens binary vector, carrying both the neomycine phosphotransferase and the e. coli β-glucuronidase gene fused to resp. the nopaline synthase and cauliflower mosaic virus 35s promotor.inoculated tuber discs produce transgenic shoots in selective media containing kanamycin. the transgenic plants are phenotypically normal and contain the euploid number of chromosomes. both the neomycin phosphotrans ... | 1988 | 24241414 |
| expression of a soybean β-conclycinin gene under the control of the cauliflower mosaic virus 35s and 19s promoters in transformed petunia tissues. | a gene encoding the α'-subunit of β-conglycinin was ligated to the 19s and 35s promoters of cauliflower mosaic virus and introduced into petunia plants on a disarmed ti-plasmid using agrobacterium tumefaciens. transformed cells were regenerated into whole plants and ummunoreactive polypeptides and hybridizable, polyadenylated mrna were detected in transformed tissues. expression from the 35s promoter was 10 to 50 times greater than expression from the 19s promoter. the level of immunodetectable ... | 1987 | 24277084 |
| transgenic plantlets of 'chancellor' grapevine (vitis sp.) from biolistic transformation of embryogenic cell suspensions. | transgenic plantlets of 'chancellor' grapevine (vitis l. complex interspecific hybrid) were produced via biolistic transformation. embryogenic cell suspensions were bombarded with 1 μm tungsten particles coated with pbi426 which encodes a fusion peptide between β-glucuronidase (gus) and neomycin phosphotransferase ii (nptii). the fusion peptide is under the control of a double 35s cauliflower mosaic virus promoter and a leader sequence from alfalfa mosaic virus. the cells were placed on kanamyci ... | 1996 | 24178348 |
| genotype- and promoter-induced variability in transient β-glucuronidase expression in pea protoplasts. | leaf mesophyll protoplasts isolated from pea (pisum sativum l.) genotypes century and pi244253 showed transient expression of β-glucuronidase (gus) when electroporated with plasmid dna containing various promoter-leader sequence constructs driving the gus gene. the optimum conditions for transient expression were: using protoplasts isolated from leaf material that had been kept in the dark for 90 h; electroporating at 250 v and 960 μf; and using 125 μg of calf thymus carrier dna and 75 μ of plas ... | 1990 | 24226370 |
| inducible and constitutive expression of an elicitor gene hrip1 from alternaria tenuissima enhances stress tolerance in arabidopsis. | hrip1 is a novel hypersensitive response-inducing protein secreted by alternaria tenuissima that activates defense responses and systemic acquired resistance in tobacco. this study investigates the role that hrip1 plays in responses to abiotic and biotic stress using transgenic arabidopsis thaliana expressing the hrip1 gene under the control of the stress-inducible rd29a promoter or constitutive cauliflower mosaic virus 35s promoter. bioassays showed that inducible hrip1 expression in rd29a∷hrip ... | 2015 | 25120219 |
| expression, purification, and biophysical characterization of a secreted anthrax decoy fusion protein in nicotiana benthamiana. | anthrax toxin receptor-mediated drug development for blocking anthrax toxin action has received much attention in recent decades. in this study, we produced a secreted anthrax decoy fusion protein comprised of a portion of the human capillary morphogenesis gene-2 (cmg2) protein fused via a linker to the fragment crystallizable (fc) domain of human immunoglobulin g1 in nicotiana benthamiana plants using a transient expression system. using the cauliflower mosaic virus (camv) 35s promoter and co-e ... | 2017 | 28054967 |
| generation of resistance to the diphenyl ether herbicide, oxyfluorfen, via expression of the bacillus subtilis protoporphyrinogen oxidase gene in transgenic tobacco plants. | in an effort to develop transgenic plants resistant to diphenyl ether herbicides, we introduced the protoporphyrinogen oxidase (ec 1.3.3.4) gene of bacillus subtilis into tobacco plants. the results from a northern analysis and leaf disc assay indicate that the expression of the b. subtilis protoporphyrinogen oxidase gene under the cauliflower mosaic virus 35s promoter generated resistance to the diphenyl ether herbicide, oxyfluorfen, in transgenic tobacco plants. | 1998 | 27315932 |
| transgenic bt cotton driven by the green tissue-specific promoter shows strong toxicity to lepidopteran pests and lower bt toxin accumulation in seeds. | a promoter of the pnzip (pharbitis nil leucine zipper) gene (1.459 kb) was cloned from pharbitis nil and fused to the gus (β-glucuronidase) and bacillus thuringiensis endotoxin (cry9c) genes. several transgenic pnzip::gus and pnzip::cry9c cotton lines were developed by agrobacterium-mediated transformation. strong gus staining was detected in the green tissues of the transgenic pnzip::gus cotton plants. in contrast, gus staining in the reproductive structures such as petals, anther, and immature ... | 2016 | 26728504 |
| divergence of host range and biological properties between natural isolate and full-length infectious cdna clone of the beet mild yellowing virus 2itb. | plant infection by poleroviruses is restricted to phloem tissues, preventing any classical leaf rub inoculation with viral rna or virions. efficient virus inoculation to plants is achieved by viruliferous aphids that acquire the virus by feeding on infected plants. the use of promoter-driven infectious cdna is an alternative means to infect plants and allows reverse genetic studies to be performed. using beet mild yellowing virus isolate 2itb (bmyv-2itb), we produced a full-length infectious cdn ... | 2014 | 23855287 |
| overexpression of populus trichocarpa cyp85a3 promotes growth and biomass production in transgenic trees. | brassinosteroids (brs) are essential hormones that play crucial roles in plant growth, reproduction and response to abiotic and biotic stress. in arabidopsis, atcyp85a2 works as a bifunctional cytochrome p450 monooxygenase to catalyze the conversion of castasterone (cs) to brassinolide (bl), a final rate-limiting step in the br biosynthetic pathway. here, we report the functional characterizations of ptcyp85a3, one of the three atcyp85a2 homologous genes from populus trichocarpa. ptcyp85a3 share ... | 2017 | 28258966 |
| a determinant of disease symptom severity is located in rna2 of broad bean wilt virus 2. | broad bean wilt virus 2 (bbwv2), which belongs to the genus fabavirus, is a destructive pathogen of many economically important horticultural and ornamental crops. in this study, we constructed infectious full-length cdna clones of two distinct isolates of bbwv2 under control of the cauliflower mosaic virus 35s promoter. bbwv2-pap1 isolated from paprika (capsicum annuum var. gulosum) induces severe disease symptoms in various pepper varieties, whereas bbwv2-rp1 isolated from red pepper (capsicum ... | 2016 | 26428303 |
| comparative study of non-enveloped icosahedral viruses size. | now, as before, transmission electron microscopy (tem) is a widely used technique for the determination of virions size. in some studies, dynamic light scattering (dls) has also been applied for this purpose. data obtained by different authors and using different methods could vary significantly. the process of tem sample preparation involves drying on the substrate, which can cause virions to undergo morphology changes. therefore, other techniques should be used for measurements of virions size ... | 2015 | 26545232 |
| the arabidopsis synaptotagmin syta regulates the cell-to-cell movement of diverse plant viruses. | synaptotagmins are a large gene family in animals that have been extensively characterized due to their role as calcium sensors to regulate synaptic vesicle exocytosis and endocytosis in neurons, and dense core vesicle exocytosis for hormone secretion from neuroendocrine cells. thought to be exclusive to animals, synaptotagmins have recently been characterized in arabidopsis thaliana, in which they comprise a five gene family. using infectivity and leaf-based functional assays, we have shown tha ... | 2014 | 25414709 |
| expression of a bifunctional green fluorescent protein (gfp) fusion marker under the control of three constitutive promoters and enhanced derivatives in transgenic grape (vitis vinifera). | activity of three constitutive promoters and enhanced derivatives in transgenic grape (vitis vinifera l. cv. thompson seedless) was characterized using a bifunctional fusion marker containing the enhanced green fluorescent protein (egfp) and neomycin phosphotransferase (nptii) genes. relative differences in transient gfp expression and stable transformation efficiencies were used to compare promoter activity. expression patterns in transformed somatic embryos revealed that the act2 promoter from ... | 2001 | 11297784 |
| a temperature-tolerant multiplex elements and genes screening system for genetically modified organisms based on dual priming oligonucleotide primers and capillary electrophoresis. | high throughput screening systems are the preferred solution to meet the urgent requirement of increasing number of genetically modified organisms (gmos). in this study, we have successfully developed a multiplex gmo element screening system with dual priming oligonucleotide (dpo) primers. this system can detect the cauliflower mosaic virus 35s (camv 35s), terminator of nopaline synthase gene (nos), figwort mosaic virus 35s (fmv 35s) promoter, neomycin phosphotransferaseii (nptii), bt cry 1ab, p ... | 2017 | 28372191 |
| three cis-regulatory motifs, auxre, mycrs1 and mycrs2, are required for modulating the auxin- and mycorrhiza-responsive expression of a tomato gh3 gene. | auxin is well known to be a key regulator that acts in almost all physiological processes during plant growth, and in interactions between plants and microbes. however, to date, the regulatory mechanisms underlying auxin-mediated plant-arbuscular mycorrhizal (am) fungi symbiosis have not been well deciphered. previously we identified a gh3 gene, slgh3.4, strongly responsive to both auxin induction and mycorrhizal symbiosis. here, we reported a refined dissection of the slgh3.4 promoter activity ... | 2017 | 28339724 |
| genome sequence of cauliflower mosaic virus identified in earwigs (doru luteipes) through a metagenomic approach. | here we report the first complete genome sequence of a cauliflower mosaic virus from brazil, obtained from the gut content of the predator earwig (doru luteipes). this virus has a genome of 8,030 nucleotides (nt) and shares 97% genome-wide identity with an isolate from argentina. | 2017 | 28302781 |
| selective autophagy limits cauliflower mosaic virus infection by nbr1-mediated targeting of viral capsid protein and particles. | autophagy plays a paramount role in mammalian antiviral immunity including direct targeting of viruses and their individual components, and many viruses have evolved measures to antagonize or even exploit autophagy mechanisms for the benefit of infection. in plants, however, the functions of autophagy in host immunity and viral pathogenesis are poorly understood. in this study, we have identified both anti- and proviral roles of autophagy in the compatible interaction of cauliflower mosaic virus ... | 2017 | 28223514 |
| fluorescent "on-off-on" switching sensor based on cdte quantum dots coupled with multiwalled carbon nanotubes@graphene oxide nanoribbons for simultaneous monitoring of dual foreign dnas in transgenic soybean. | with the increasing concern of potential health and environmental risk, it is essential to develop reliable methods for transgenic soybean detection. herein, a simple, sensitive and selective assay was constructed based on homogeneous fluorescence resonance energy transfer (fret) between cdte quantum dots (qds) and multiwalled carbon nanotubes@graphene oxide nanoribbons (mwcnts@gonrs) to form the fluorescent "on-off-on" switching for simultaneous monitoring dual target dnas of promoter cauliflow ... | 2017 | 28182975 |
| comparison of the impact of viral and plant-derived promoters regulating selectable marker gene on maize transformation and transgene expression. | the choice of promoter regulating the selectable marker gene impacts transformation efficiency, copy number and the expression of selectable marker and flanking genes in maize. viral or plant-derived constitutive promoters are often used to regulate selectable marker genes. we compared two viral promoters, cauliflower mosaic virus (camv 35t) and sugarcane bacilliform virus (scbv) with two plant promoters, rice actin1 (osact1) and maize ubiquitin 1 (zmubi1) to drive aryloxyalkanoate dioxygenase ( ... | 2017 | 28160062 |
| a dna probe based on phosphorescent resonance energy transfer for detection of transgenic 35s promoter dna. | a qds-dna nano-probe was made by combining mn-doped zns room-temperature phosphorescence (rtp) quantum dots (qds) and dna. then an rtp sensor for quantitative detection of genetically-modified mark sequence cauliflower mosaic virus 35s promoter (ca mv 35s) dna was built on basis of phosphorescent resonance energy transfer (pret). the underlying principles were that a qds-dna water-soluble nano-probe was built by connecting single-strand dna to the surfaces of qds via a ligand exchange method. th ... | 2017 | 28088748 |
| ectopic expression of an apple cytochrome p450 gene mdcypm1 negatively regulates plant photomorphogenesis and stress response in arabidopsis. | cytochrome p450s play an important role in plant growth and are involved in multiple stresses response. however, little is known about the functions of cytochrome p450s in apple. here, a malus × domestica cytochrome p450 monooxygenase 1 gene, mdcypm1, was identified and subsequently cloned from apple 'gala' (malus × domestica). to verify the functions of mdcypm1, we generated transgenic arabidopsis plants expressing the apple mdcypm1 gene under the control of the cauliflower mosaic virus 35s pro ... | 2017 | 28073698 |
| synthetic introns help identify sequences in the 5' utr intron of the glycine max polyubiquitin (gmubi) promoter that give increased promoter activity. | specific sequences within the leader intron of a soybean polyubiquitin gene stimulated gene expression when placed either within a synthetic intron or upstream of a core promoter. the intron in the 5' untranslated region of the soybean polyubiquitin promoter, gmubi, seems to contribute to the high activity of this promoter. to identify the stimulatory sequences within the intron, ten different sequential intronic sequences of 40 nt were isolated, cloned as tetrameric repeats and placed upstream ... | 2017 | 28070655 |
| generation of gene-edited chrysanthemum morifolium using multi-copy transgenes as targets and markers. | the most widely used gene editing technology-the crispr/cas9 system-employs a bacterial monomeric dna endonuclease known as clustered regularly interspaced short palindromic repeats (crispr)-associated protein 9 (cas9) and single-guide rna (sgrna) that directs cas9 to a complementary target dna. however, introducing mutations into higher polyploid plant species, especially for species without genome information, has been difficult. chrysanthemum morifolium (chrysanthemum) is one of the most impo ... | 2017 | 28049122 |
| use of a novel metal indicator to judge loop-mediated isothermal amplification for detecting the 35s promoter. | loop-mediated isothermal amplification (lamp) is a widely used isothermal nucleic acid amplification method. here we developed a new closed-tube colorimetric method for judging lamp with a novel metal indicator. first, the metal indicator, acid chrome blue k (acbk), was evaluated in the lamp reaction with various combinations of reaction reagents, such as reaction buffer, dntp mixtures, primer mixtures, or mg(2+). we found that the solution color of the lamp reaction with acbk changed from red t ... | 2017 | 27873004 |
| electroactive crown ester-cu(2+) complex with in-situ modification at molecular beacon probe serving as a facile electrochemical dna biosensor for the detection of camv 35s. | a novel electrochemical dna biosensor has been facilely constructed by in-situ assembly of electroactive 4'-aminobenzo-18-crown-6-copper(ii) complex (abc-cu(2+)) on the free terminal of the hairpin-structured molecule beacon. the 3'-sh modified molecule beacon probe was first immobilized on the gold electrode (aue) surface through self-assembly chemistry of au-s bond. then the crow ester of abc was covalently coupled with 5'-cooh on the molecule beacon, and served as a platform to attach the cu( ... | 2017 | 27829553 |
| barley hvpaphy_a as transgene provides high and stable phytase activities in mature barley straw and in grains. | the phytase purple acid phosphatase (hvpaphy_a) expressed during barley seed development was evaluated as transgene for overexpression in barley. the phytase was expressed constitutively driven by the cauliflower mosaic virus 35s-promoter, and the phytase activity was measured in the mature grains, the green leaves and in the dry mature vegetative plant parts left after harvest of the grains. the t2 -generation of hvpaphy_a transformed barley showed phytase activity increases up to 19-fold (29 0 ... | 2017 | 27633382 |
| photoelectrochemical camv35s biosensor for discriminating transgenic from non-transgenic soybean based on sio2@cdte quantum dots core-shell nanoparticles as signal indicators. | a methodology for detection of the cauliflower mosaic virus 35s(camv35s) promoter was developed to distinguish transgenic from non-transgenic soybean samples by using photoelectrochemical (pec) biosensor. in this pec biosensing system, the as-prepared gold nanoparticles-reduced graphene oxide acted as a nanocarrier to immobilize the thiol-functional probe (probe1), and the sio2@cdte quantum dots (qds) core-shell nanoparticles tagged with the amino-functional probe (probe2) acted as signal indica ... | 2016 | 27769398 |
| a robotic platform for high-throughput protoplast isolation and transformation. | over the last decade there has been a resurgence in the use of plant protoplasts that range from model species to crop species, for analysis of signal transduction pathways, transcriptional regulatory networks, gene expression, genome-editing, and gene-silencing. furthermore, significant progress has been made in the regeneration of plants from protoplasts, which has generated even more interest in the use of these systems for plant genomics. in this work, a protocol has been developed for autom ... | 2016 | 27768035 |
| construction of a full-length infectious cdna clone of cowpea mild mottle virus. | infectious cdna clones are an important tool to study the molecular and cellular process of rna virus infection. in vitro and in vivo transcription systems are the two main strategies used in the generation of infectious cdna clones for rna viruses. this study describes the first generation of a full-length infectious cdna clone of cowpea mild mottle virus (cpmmv), a carlavirus. the full-length genome was synthesized by overlap extension pcr of two overlapping fragments and cloned in a puc-based ... | 2017 | 27730428 |
| 35s promoter methylation in kanamycin-resistant kalanchoe (kalanchoe pinnata l.) plants expressing the antimicrobial peptide cecropin p1 transgene. | transgenic kalanchoe plants (kalanchoe pinnata l.) expressing the antimicrobial peptide cecropin p1 gene (cecp1) under the control of the 35s cauliflower mosaic virus 35s rna promoter and the selective neomycin phosphotransferase ii (nptii) gene under the control of the nopaline synthase gene promoter were studied. the 35s promoter methylation and the cecropin p1 biosynthesis levels were compared in plants growing on media with and without kanamycin. the low level of active 35s promoter methylat ... | 2016 | 27682168 |
| a viral satellite dna vector-induced transcriptional gene silencing via dna methylation of gene promoter in nicotiana benthamiana. | virus-induced gene silencing (vigs) has been widely used for plant functional genomics study at the post-transcriptional level using various dna or rna viral vectors. however, while virus-induced transcriptional gene silencing (vitgs) via dna methylation of gene promoter was achieved using several plant rna viral vectors, it has not yet been done using a satellite dna viral vector. in this study, a viral satellite dna associated with tomato yellow leaf curl china virus (tylccnv), which has been ... | 2016 | 27422476 |
| expression of cadr enhances its specific activity for cd detoxification and accumulation in arabidopsis. | cadmium (cd) is a transition metal that is highly toxic in biological systems. anthropogenic emissions of cd have increased biogeochemical cycling and the amount of cd in the biosphere. here we studied the utility of a bacterial cd-binding protein, cadr, for the remediation of cd contamination. cadr was successfully targeted to chloroplasts using a constitutive cauliflower mosaic virus (camv) 35s promoter or a shoot-specific chl a/b-binding protein 2 gene (cab2) promoter and an rbcs (small subun ... | 2016 | 27382127 |
| the role of plasmodesma-located proteins in tubule-guided virus transport is limited to the plasmodesmata. | intercellular spread of plant viruses involves passage of the viral genome or virion through a plasmodesma (pd). some viruses severely modify the pd structure, as they assemble a virion carrying tubule composed of the viral movement protein (mp) inside the pd channel. successful modulation of the host plant to allow infection requires an intimate interaction between viral proteins and both structural and regulatory host proteins. to date, however, very few host proteins are known to promote viru ... | 2016 | 27339685 |
| gene expression and promoter analysis of a novel tomato aldo-keto reductase in response to environmental stresses. | the functional role of an uncharacterized tomato (solanum lycopersicum) aldo-keto reductase 4b, denoted as slakr4b, was investigated. the gene expression of tomato slakr4b was detected at a high level in the senescent leaves and the ripening fruits of tomato. although d-galacturonic acid reductase activities tended to be higher in tomato slakr4b-overexpressing transgenic tobacco by-2 cell lines than those in control cell lines, slakr4b gene expression was not well correlated with l-ascorbic acid ... | 2016 | 27337067 |
| detection of herbaceous-plant pararetrovirus in lichen herbarium samples. | cauliflower mosaic virus (camv) - a plant pararetrovirus that naturally causes diseases in brassicaceae and solanaceae plant hosts worldwide - has been detected by pcr for the first time in herbarium samples of usnea sp. lichens. the virus's presence in these lichens did not result in any micro- or macromorphological changes, and the herbarium records were classified as representative for the distinct species. sequence analyses classified all the detected viruses into one lineage of camv isolate ... | 2016 | 27265470 |
| ectopic expression of jatropha curcas apetala1 (jcap1) caused early flowering in arabidopsis, but not in jatropha. | jatropha curcas is a promising feedstock for biofuel production because jatropha oil is highly suitable for the production of biodiesel and bio-jet fuels. however, jatropha exhibits a low seed yield as a result of unreliable and poor flowering. apetala1 (ap1) is a floral meristem and organ identity gene in higher plants. the flower meristem identity genes of jatropha have not yet been identified or characterized. to better understand the genetic control of flowering in jatropha, an ap1 homolog ( ... | 2016 | 27168978 |
| viral protein suppresses oxidative burst and salicylic acid-dependent autophagy and facilitates bacterial growth on virus-infected plants. | virus interactions with plant silencing and innate immunity pathways can potentially alter the susceptibility of virus-infected plants to secondary infections with nonviral pathogens. we found that arabidopsis plants infected with cauliflower mosaic virus (camv) or transgenic for camv silencing suppressor p6 exhibit increased susceptibility to pseudomonas syringae pv. tomato (pst) and allow robust growth of the pst mutant hrcc-, which cannot deploy effectors to suppress innate immunity. the impa ... | 2016 | 27120694 |
| generation of novel floral traits using a combination of floral organ-specific promoters and a chimeric repressor in torenia fournieri lind. | in this study, we attempted to develop a new biotechnological method for the efficient modification of floral traits. because transcription factors play an important role in determining floral traits, chimeric repressors, which are generated by attaching a short transcriptional repressor domain to transcription factors, have been widely used as effective tools for modifying floral traits in many plant species. however, the overexpression of these chimeric repressors by the cauliflower mosaic vir ... | 2016 | 27107289 |
| a chimeric repressor of petunia ph4 r2r3-myb family transcription factor generates margined flowers in torenia. | the development of new phenotypes is key to the commercial development of the main floricultural species and cultivars. important new phenotypes include features such as multiple-flowers, color variations, increased flower size, new petal shapes, variegation and distinctive petal margin colourations. although their commercial use is not yet common, the transgenic technologies provide a potentially rapid means of generating interesting new phenotypes. in this report, we construct 5 vectors which ... | 2016 | 27089475 |
| identification and functional characterization of the nac gene promoter from populus euphratica. | the penac1 promoter is a non-tissue-specific and stress-inducible promoter containing a ga-responsive element and a myb recognition sequence that are responsible for induced expression patterns. nac transcription factors play vital roles in complex signaling networks during plant stress responses. promoters as crucial molecular switches are involved in the transcriptional regulation of gene activities dynamic network controlling a variety of biological processes, such as developmental processes, ... | 2016 | 27084679 |
| comment on "large bottleneck size in cauliflower mosaic virus populations during host plant colonization" by monsion et al. (2008). | | 2016 | 27078639 |
| [evaluation of salt tolerance of transgenic tobacco plants bearing with p5cs1 gene of arabidopsis thaliana]. | arabidopsis thaliana delta1-pyrroline-5-carhoxylate synthase 1 gene (p5cs1) cdna was cloned under the control of the potent constitutive 35s rna promoter of the cauliflower mosaic virus and transferred into genome of tobacco cv. petit havana sr-1 (nicotiana tabacum l.) plants. it is shown that the constitutive level of proline in the transgenic plants t0 exceeds that of the sr1 reference line by 1.5 to 4 times. under conditions of salt stress (200, 300 mm nacl) t1-generation transgenic plants in ... | 2015 | 27055296 |
| infectious clones of the crinivirus cucurbit chlorotic yellows virus are competent for plant systemic infection and vector transmission. | cucurbit chlorotic yellows virus (ccyv), a recently identified bipartite crinivirus, causes economic losses in cucurbit plants. ccyv is naturally transmitted only by whitefly bemisia tabaci. here we constructed full-length cdna clones of ccyv (rna1 and rna2) fused to the t7 rna polymerase promoter and the cauliflower mosaic virus 35s promoter. ccyv replicated and accumulated efficiently in cucumis sativus protoplasts transfected with in vitro transcripts. without rna2, rna1 replicated efficientl ... | 2016 | 26982585 |
| overexpression of nakr3 enhances salt tolerance in arabidopsis. | salinity is a major abiotic stress in agriculture. here, we report that sodium potassium root defective3 (nakr3), which encodes a heavy metal-associated domain protein, is involved in salt tolerance in arabidopsis. the results of quantitative reverse transcription-polymerase chain reaction analysis revealed that nakr3 was induced by high salinity and osmotic stresses, but not by cu(2+) stress. transient expression of nakr3-gfp in arabidopsis protoplasts showed that the nakr3 protein was localize ... | 2016 | 26909945 |
| noncoding rnas of plant viruses and viroids: sponges of host translation and rna interference machinery. | noncoding sequences in plant viral genomes are well-known to control viral replication and gene expression in cis. however, plant viral and viroid noncoding (nc)rna sequences can also regulate gene expression acting in trans, often acting like 'sponges' that bind and sequester host cellular machinery to favor viral infection. noncoding sequences of small subgenomic (sg)rnas of barley yellow dwarf virus (bydv) and red clover necrotic mosaic virus (rcnmv) contain a cap-independent translation elem ... | 2016 | 26900786 |
| tolerance to excess-boron conditions acquired by stabilization of a bor1 variant with weak polarity in arabidopsis. | boron (b) is a metalloid that is essential for plant growth but is toxic when present in excess. arabidopsis bor1 is a borate exporter, facilitating b translocation from root to shoot under limited-b conditions. bor1 shows stele side polar localization in the plasma membrane of various root cells, presumably to support b translocation toward the stele. bor1 is degraded under high-b supply through vacuolar sorting via ubiquitination at the k590 residue to prevent the accumulation of b to a toxic ... | 2016 | 26870730 |
| overexpression of an apocynum venetum dead-box helicase gene (avdh1) in cotton confers salinity tolerance and increases yield in a saline field. | soil salinity is a major environmental stress limiting plant growth and productivity. we have reported previously the isolation of an apocynum venetum dead-box helicase 1 (avdh1) that is expressed in response to salt exposure. here, we report that the overexpression of avdh1 driven by a constitutive cauliflower mosaic virus-35s promoter in cotton plants confers salinity tolerance. southern and northern blotting analyses showed that the avdh1 gene was integrated into the cotton genome and express ... | 2015 | 26779246 |
| salicylic acid treatment and expression of an rna-dependent rna polymerase 1 transgene inhibit lethal symptoms and meristem invasion during tobacco mosaic virus infection in nicotiana benthamiana. | host rna-dependent rna polymerases (rdrs) 1 and 6 contribute to antiviral rna silencing in plants. rdr6 is constitutively expressed and was previously shown to limit invasion of nicotiana benthamiana meristem tissue by potato virus x and thereby inhibit disease development. rdr1 is inducible by salicylic acid (sa) and several other phytohormones. but although it contributes to basal resistance to tobacco mosaic virus (tmv) it is dispensable for sa-induced resistance in inoculated leaves. the lab ... | 2016 | 26757721 |
| myb transcription factor isolated from raphanus sativus enhances anthocyanin accumulation in chrysanthemum cultivars. | a myb transcription factor gene, rsmyb1, from radish was introduced into the chrysanthemum cultivars 'peach nd', 'peach red', and 'vivid scarlet' under the control of the cauliflower mosaic virus 35s promoter. presence of rsmyb1 in transgenic lines was confirmed using polymerase chain reaction (pcr). results of reverse-transcription-pcr analysis revealed that the expression of rsmyb1 was stable in all transgenic lines and could enhance the expression levels of three key biosynthetic genes (f3h, ... | 2016 | 28330149 |
| isolation and characterization of "gmscream" promoters that regulate highly expressing soybean (glycine max merr.) genes. | to increase our understanding of the regulatory components that control gene expression, it is important to identify, isolate and characterize new promoters. in this study, a group of highly expressed soybean (glycine max merr.) genes, which we have named "gmscream", were first identified from rna-seq data. the promoter regions were then identified, cloned and fused with the coding region of the green fluorescent protein (gfp) gene, for introduction and analysis in different tissues using 3 tool ... | 2015 | 26706070 |
| a model for intracellular movement of cauliflower mosaic virus: the concept of the mobile virion factory. | the genomes of many plant viruses have a coding capacity limited to <10 proteins, yet it is becoming increasingly clear that individual plant virus proteins may interact with several targets in the host for establishment of infection. as new functions are uncovered for individual viral proteins, virologists have realized that the apparent simplicity of the virus genome is an illusion that belies the true impact that plant viruses have on host physiology. in this review, we discuss our evolving u ... | 2016 | 26687180 |
| establishment of an agrobacterium-mediated inoculation system for cucumber green mottle mosaic virus. | the infectious full-length cdna clones of cucumber green mottle mosaic virus (cgmmv) isolates kw and kom, which were isolated from watermelon and oriental melon, respectively, were constructed under the control of the cauliflower mosaic virus 35s promoter. we successfully inoculated nicotiana benthamiana with the cloned cgmmv isolates kw and kom by agrobacterium-mediated infiltration. virulence and symptomatic characteristics of the cloned cgmmv isolates kw and kom were tested on several indicat ... | 2015 | 26674677 |
| development of a new vector using soybean yellow common mosaic virus for gene function study or heterologous protein expression in soybeans. | a new vector using soybean yellow common mosaic virus (sycmv) was constructed for gene function study or heterologous protein expression in soybeans. the in vitro transcript with a 5' cap analog m7gpppg from an sycmv full-length infectious vector driven by a t7 promoter infected soybeans (psycmvt7-full). the symptoms observed in the soybeans infected with either the sap from sycmv-infected leaves or psycmvt7-full were indistinguishable, suggesting that the vector exhibits equivalent biological a ... | 2016 | 26569351 |
| overexpression of a phytophthora cytoplasmic crn effector confers resistance to disease, salinity and drought in nicotiana benthamiana. | the crinkler (crn) effector family is produced by oomycete pathogens and may manipulate host physiological and biochemical events inside host cells. here, pscrn161 was identified from phytophthora sojae based on its broad and strong cell death suppression activities. the effector protein contains two predicted nuclear localization signals and localized to nuclei of plant cells, indicating that it may target plant nuclei to modify host cell physiology and function. the chimeric gene gfp:pscrn161 ... | 2015 | 26546319 |
| functional analysis of cotton leaf curl kokhran virus/cotton leaf curl multan betasatellite rna silencing suppressors. | in south asia, cotton leaf curl disease (clcud) is caused by a complex of phylogenetically-related begomovirus species and a specific betasatellite, cotton leaf curl multan betasatellite (clcumub). the post-transcriptional gene silencing (ptgs) suppression activities of the transcriptional activator protein (trap), c4, v2 and βc1 proteins encoded by cotton leaf curl kokhran virus (clcukov)/clcumub were assessed in nicotiana benthamiana. a variable degree of local silencing suppression was observ ... | 2015 | 26512705 |
| complementary dna cloning of the pear 1-aminocyclopropane-1-carboxylic acid oxidase gene and agrobacterium-mediated anti-sense genetic transformation. | the aim of the present study was to genetically modify plantlets of the chinese yali pear to reduce their expression of ripening-associated 1-aminocyclopropane-1-carboxylic acid oxidase (aco) and therefore increase the shelf-life of the fruit. primers were designed with selectivity for the conserved regions of published aco gene sequences, and yali complementary dna (cdna) cloning was performed by reverse transcription quantitative polymerase chain reaction (pcr). the obtained cdna fragment cont ... | 2015 | 26460204 |
| cloning and expressing trypsin modulating oostatic factor in chlorella desiccata to control mosquito larvae. | the insect peptide hormone trypsin modulating oostatic factor (tmof), a decapeptide that is synthesized by the mosquito ovary and controls the translation of the gut's trypsin mrna was cloned and expressed in the marine alga chlorella desiccata. to express aedes aegypti tmof gene (tmfa) in c. desiccata cells, two plasmids (pyes2/tmof and pydb4-tmfa) were engineered with pkylx71 dna (5 kb) carrying the cauliflower mosaic virus (camv) promoter 35s(2) and the kanamycin resistant gene (neo), as well ... | 2016 | 26440910 |
| development of gateway binary vector series with four different selection markers for the liverwort marchantia polymorpha. | we previously reported agrobacterium-mediated transformation methods for the liverwort marchantia polymorpha using the hygromycin phosphotransferase gene as a marker for selection with hygromycin. in this study, we developed three additional markers for m. polymorpha transformation: the gentamicin 3'-acetyltransferase gene for selection with gentamicin; a mutated acetolactate synthase gene for selection with chlorsulfuron; and the neomycin phosphotransferase ii gene for selection with g418. base ... | 2015 | 26406247 |
| camv-35s promoter sequence-specific dna methylation in lettuce. | we found 35s promoter sequence-specific dna methylation in lettuce. additionally, transgenic lettuce plants having a modified 35s promoter lost methylation, suggesting the modified sequence is subjected to the methylation machinery. we previously reported that cauliflower mosaic virus 35s promoter-specific dna methylation in transgenic gentian (gentiana triflora × g. scabra) plants occurs irrespective of the copy number and the genomic location of t-dna, and causes strong gene silencing. to conf ... | 2016 | 26373653 |
| water-soluble chlorophyll-binding proteins from arabidopsis thaliana and raphanus sativus target the endoplasmic reticulum body. | non-photosynthetic chlorophyll (chl) proteins called water-soluble chl-binding proteins are distributed in brassicaceae plants. brassica oleracea wscp (bowscp) and lepidium virginicum wscp (lvwscp) are highly expressed in leaves and stems, while arabidopsis thaliana wscp (atwscp) and raphanus sativus wscp (rshwscp) are highly transcribed in floral organs. bowscp and lvwscp exist in the endoplasmic reticulum (er) body. however, the subcellular localization of atwscp and rshwscp is still unclear. ... | 2015 | 26289422 |
| dna extraction techniques compared for accurate detection of genetically modified organisms (gmos) in maize food and feed products. | in this paper, dna extraction methods have been evaluated to detect the presence of genetically modified organisms (gmos) in maize food and feed products commercialised in turkey. all the extraction methods tested performed well for the majority of maize foods and feed products analysed. however, the highest dna content was achieved by the wizard, genespin or the ctab method, all of which produced optimal dna yield and purity for different maize food and feed products. the samples were then scre ... | 2015 | 26243938 |
| retraction for moissiard and voinnet, rna silencing of host transcripts by cauliflower mosaic virus requires coordinated action of the four arabidopsis dicer-like proteins. | | 2015 | 26216957 |
| targeting helicase-dependent amplification products with an electrochemical genosensor for reliable and sensitive screening of genetically modified organisms. | cultivation of genetically modified organisms (gmos) and their use in food and feed is constantly expanding; thus, the question of informing consumers about their presence in food has proven of significant interest. the development of sensitive, rapid, robust, and reliable methods for the detection of gmos is crucial for proper food labeling. in response, we have experimentally characterized the helicase-dependent isothermal amplification (hda) and sequence-specific detection of a transgene from ... | 2015 | 26198403 |
| cauliflower mosaic virus transcriptome reveals a complex alternative splicing pattern. | the plant pararetrovirus cauliflower mosaic virus (camv) uses alternative splicing to generate several isoforms from its polycistronic pregenomic 35s rna. this pro-cess has been shown to be essential for infectivity. previous works have identified four splice donor sites and a single splice acceptor site in the 35s rna 5' region and suggested that the main role of camv splicing is to downregulate expression of open reading frames (orfs) i and ii. in this study, we show that alternative splicing ... | 2015 | 26162084 |
| complete genome sequence and construction of infectious full-length cdna clones of tobacco ringspot nepovirus, a viral pathogen causing bud blight in soybean. | tobacco ringspot virus (trsv, genus nepovirus), causes severe diseases in soybean and tobacco plants. trsv-induced bud blight disease significantly reduced both the yield and quality of soybeans. the function of the encoded viral gene product involved in trsv infection was unclear due to the limitation of reverse genetics studies on the viral genome. here, we represent the successful construction of infectious full-length cdna clones of trsv genome (rna1 and rna2). the cdnas of trsv rna1 and rna ... | 2015 | 26159876 |
| ft overexpression induces precocious flowering and normal reproductive development in eucalyptus. | eucalyptus trees are among the most important species for industrial forestry worldwide. however, as with most forest trees, flowering does not begin for one to several years after planting which can limit the rate of conventional and molecular breeding. to speed flowering, we transformed a eucalyptus grandis × urophylla hybrid (sp7) with a variety of constructs that enable overexpression of flowering locus t (ft). we found that ft expression led to very early flowering, with events showing flor ... | 2016 | 26132805 |
| rna based viral silencing suppression in plant pararetroviruses. | the 35s promoter of cauliflower mosaic virus and that of other plant pararetroviruses gives rise to an rna, which is both a pre-genome and a polycistronic mrna. the 600 nucleotide long very structured leader of this rna is also transcribed separately. the resulting 8s rna is then converted to a double strand giving rise to a huge set of sirnas, which suppress silencing. in this mini-review i discuss how this versatile stretch of 600 nts constitutes a masterpiece of evolution. | 2015 | 26113850 |