| prevalence of jc virus in chinese patients with colorectal cancer. | jcv is a dna polyomavirus very well adapted to humans. although jcv dna has been detected in colorectal cancers (crc), the association between jcv and crc remains controversial. in china, the presence of jcv infection in crc patients has not been reported. here, we investigated jcv infection and viral dna load in chinese crc patients and to determine whether the jcv dna in peripheral blood (pb) can be used as a diagnostic marker for jcv-related crc. | 2012 | 22606241 |
| a peptide talk between jc virus and the human host: from silent infection to autoimmunity. | analysis of jc virus (jcv) polyprotein for peptide sharing with the human proteome reveals that the virus has hundreds of pentapeptide sequences in common with the human proteins. the datum is interesting in light of the fundamental role exerted by short amino acid sequences in protein-protein interactions and, consequently, in biochemical reactions and immune recognition. searching for new approaches to understand the jcv infection scenarios, from the immunoevasion phenomenon underlying the vir ... | 2012 | 22594935 |
| anti-jc virus antibodies in a large german natalizumab-treated multiple sclerosis cohort. | to investigate the rate of seropositivity of anti-jc virus (jcv) antibodies in a german multiple sclerosis (ms) cohort treated with natalizumab in the postmarketing setting and to assess anti-jcv serostatus in samples obtained before diagnosis of progressive multifocal leukoencephalopathy (pml). | 2012 | 22592369 |
| risk of natalizumab-associated progressive multifocal leukoencephalopathy. | progressive multifocal leukoencephalopathy (pml) is associated with natalizumab treatment. we quantified the risk of pml in patients with multiple sclerosis, according to the presence or absence of three risk factors: positive status with respect to anti-jc virus antibodies, prior use of immunosuppressants, and increasing duration of natalizumab treatment. | 2012 | 22591293 |
| urinary jcv-dna testing during natalizumab treatment may increase accuracy of pml risk stratification. | the risk of progressive multifocal leukoencephalopathy (pml) in patients treated with natalizumab for multiple sclerosis (ms) is a serious concern. the presence of anti-jc virus antibodies is a risk factor for pml development, but 2.5 % of the patients result falsely-negative, while the prognostic relevance of testing jcv-dna in biological fluids of treated patients is debated. aim of this work was to evaluate the utility of testing jcv-dna, together with anti-jcv antibodies, in biological sampl ... | 2012 | 22585413 |
| disease-modifying drugs for multiple sclerosis and jc virus expression. | natalizumab-associated progressive multifocal leukoencephalopathy in multiple sclerosis (ms) occurred in two individuals also treated with interferon β1a, raising concerns about the interaction of these disease-modifying agents and leading to the recommendation to avoid their concomitant administration. however, type i interferons are antiviral. using a real-time quantitative polymerase chain reaction for the detection and quantification of the john cunningham virus (jcv), dna in peripheral bloo ... | 2012 | 22585288 |
| neurologic complications in patients with inflammatory bowel disease: increasing relevance in the era of biologics. | extraintestinal manifestations of inflammatory bowel disease (ibd) can involve almost every organ system. neurologic complications are not infrequent in patients with ibd, but are poorly recognized and underreported. pathophysiologically, these may represent immune-mediated phenomenon, nonimmunologic complications due to micronutrient deficiencies, thromboembolism, or be medication-induced. peripheral neuropathy is the most common neurologic complication of ibd and may be immune-mediated, or cau ... | 2013 | 22552994 |
| jc virus: more than 40 years later with new implications. | | 2017 | 22552450 |
| increased program cell death-1 expression on t lymphocytes of patients with progressive multifocal leukoencephalopathy. | the cellullar immune response is important in the containment of progressive multifocal leukoencephalopathy (pml). we examined program cell death-1 (pd-1) expression, a marker of cellular immune exhaustion, on t lymphocytes in pml. pd-1 expression was elevated on total cd4(+) and cd8(+) t cells (medians 36% and 24%) in pml patients compared with healthy control subjects (medians 14% and 18%; p = 0.0015 and p = 0.033). in pml patients, jc virus (jcv)-specific cd8(+) cytotoxic t lymphocytes expres ... | 2012 | 22549384 |
| simultaneous pml-iris after discontinuation of natalizumab in a patient with ms. | progressive multifocal leukoencephalopathy (pml) is a severe complication of natalizumab therapy in patients with multiple sclerosis (ms), which is often accompanied by an immune reconstitution inflammatory syndrome (iris) after removal of the drug. we describe a patient with ms who presented with simultaneous pml-iris 2 months after stopping natalizumab for other reasons. | 2012 | 22517104 |
| the vp1 subunit of jc polyomavirus recapitulates early events in viral trafficking and is a novel tool to study polyomavirus entry. | jc polyomavirus (jcv) is an important human pathogen that causes the fatal demyelinating disease progressive multifocal leukoencephalopathy (pml). in this study we further delineate the early events of jcv entry in human glial cells and demonstrate that a pentameric subunit of the viral capsid is able to recapitulate early events in viral trafficking. we show that jcv traffics to the endoplasmic reticulum (er) by 6h post infection, and that vp1 pentamers arrive at the er with similar kinetics. f ... | 2012 | 22516137 |
| jc virus t-antigen regulates glucose metabolic pathways in brain tumor cells. | recent studies have reported the detection of the human neurotropic virus, jcv, in a significant population of brain tumors, including medulloblastomas. accordingly, expression of the jcv early protein, t-antigen, which has transforming activity in cell culture and in transgenic mice, results in the development of a broad range of tumors of neural crest and glial origin. evidently, the association of t-antigen with a range of tumor-suppressor proteins, including p53 and prb, and signaling molecu ... | 2012 | 22496891 |
| molecular prevalence of jc virus in tehran, iran. | since data about prevalence of jc virus in iranian population is scarce, this study was designed to evaluate the prevalence of jc virus in healthy individuals who had attended fajr hospital and farjam clinical laboratory in tehran, iran. | 2012 | 24834205 |
| nuclear irs-1 and cancer. | the family of insulin receptor substrates (irs) consists of four proteins (irs-1-irs-4), which were initially characterized as typical cytosolic adaptor proteins involved in insulin receptor (ir) and insulin-like growth factor i receptor (igf-ir) signaling. the first cloned and characterized member of the irs family, irs-1, has a predicted molecular weight of 132 kda, however, as a result of its extensive serine phosphorylation it separates on a sds gel as a band of approximately 160-185 kda. in ... | 2012 | 22454254 |
| from injection therapies to natalizumab: views on the treatment of multiple sclerosis. | discoveries of the mechanisms that underlie the pathogenesis of multiple sclerosis have been acquired at an impressive rate over the last few decades and, as a consequence, a growing number of treatments are becoming available for this disease. this review first analyzes the experience from the early stages of the disease-modifying therapies, then, expanding on the concept of early treatment for improved outcomes, it focuses on natalizumab and its major complication, progressive multifocal leuko ... | 2012 | 22435074 |
| emerging disease-modifying therapies in multiple sclerosis. | the past two decades have seen tremendous expansion in therapeutic options for multiple sclerosis (ms). while the growing armamentarium of therapies provides physicians and patients an array of available options, it also brings in its wake the challenging responsibility of choosing the optimal therapy for an individual patient. in a newly diagnosed patient with relapsing disease, the current practice is to start one of the interferons (interferon-beta) or glatiramer acetate. with increasing expe ... | 2012 | 22426573 |
| reactivation of human polyomavirus jc in patients affected by psoriasis vulgaris and psoriatic arthritis and treated with biological drugs: preliminary results. | psoriasis vulgaris (psv) and psoriatic arthritis (psa) are inter-related heritable inflammatory skin diseases. psoriatic lesions develop as a result of abnormal immune responses, hyperproliferation and altered differentiation of keratinocytes, and a notable subset of psoriatic patients develops psa, characterized by joints inflammation. recently, biological drugs were introduced to treat these diseases. however, this therapy has already been associated with the development of serious life-threat ... | 2012 | 22422468 |
| intravesicular cidofovir for the treatment of polyomavirus-associated hemorrhagic cystitis. | to review the literature regarding the use of intravesicular cidofovir in the treatment of polyomavirus-associated hemorrhagic cystitis. | 2012 | 22395246 |
| natalizumab discontinuation after pml risk stratification: outcome from a shared and informed decision. | multifocal progressive leukoencephalopathy (pml) is associated with jc virus (jcv) seropositivity, past immunosuppression, and natalizumab treatment for two years or more. the aim of our study was to investigate the rate of treatment discontinuation after stratifying for the three risk factors in a group of 104 natalizumab-treated patients with relapsing-remitting multiple sclerosis. we investigated jcv serological status in our population. we then divided patients into groups according to their ... | 2012 | 22383232 |
| progressive multifocal leukoencephalopathy in a patient treated with etanercept. | recently, increased attention has been paid to the association of progressive multifocal leukoencephalopathy (pml) with the use of immunomodulatory medications for autoimmune diseases. | 2012 | 22367837 |
| [other possible clinical applications of drugs with 5ht2a effect in liaison psychiatry: cases report]. | in liaison psychiatry it is possible to get an integral view of patient's treatment and needs, paying special attention to pharmacological interactions and contraindications. some particular cases motivated the description, report and review about other possible applications of 5ht2a and 5ht3 antagonist, particularly mirtazapine and olanzapine, in hyperalgesia syndrome, tinnitus and progressive multifocal leukoencephalopathy by jc virus. | 2012 | 26573480 |
| risk stratification for progressive multifocal leukoencephalopathy in patients treated with natalizumab. | natalizumab is a highly effective immunomodulator in the treatment of multiple sclerosis (ms). treatment with natalizumab has been associated with progressive multifocal leukoencephalopathy (pml), an infection of the central nervous system (cns) caused by a pathogenic form of the normally benign jc virus (jcv). we searched pubmed and used current data from the natalizumab global safety database to assess risk factors and quantify the risk of pml. natalizumab treatment duration and prior use of i ... | 2012 | 22312009 |
| assessment of the risk of polyomavirus jc reactivation in patients with immune-mediated diseases during long-term treatment with infliximab. | polyomavirus jc (jcv) reactivation causing progressive multifocal leukoencephalopathy is a main concern during biological therapies. here, jcv reactivation in patients suffering from immune-mediated diseases after a long-term treatment with anti-tumor necrosis factor alpha (tnf-α) inhibitor infliximab was investigated. peripheral mononuclear blood cells (pbmc), plasma and urine samples were obtained from 61 immune-mediated diseases patients treated or not with infliximab in combination with ster ... | 2012 | 22281875 |
| jc virus intranuclear inclusions associated with pml-nbs: analysis by electron microscopy and structured illumination microscopy. | progressive multifocal leukoencephalopathy is a fatal demyelinating disorder caused by jc virus infection. jc virus was recently found to target promyelocytic leukemia nuclear bodies (pml-nbs), punctuate domains in the nuclei. thus, the virus progenies cluster in dots as intranuclear inclusions (ie, as dot-shaped inclusions). in the present study, both the viral major and minor capsid proteins were expressed from polycistronic expression vectors with a powerful promoter, and formation into virus ... | 2012 | 22266251 |
| firategrast for relapsing remitting multiple sclerosis: a phase 2, randomised, double-blind, placebo-controlled trial. | monoclonal antibody therapy against α4β-integrin is efficacious in patients with multiple sclerosis (ms) with some safety concerns. we assessed the safety and efficacy of firategrast, a small oral anti-α4β-integrin molecule, in patients with relapsing remitting ms. | 2012 | 22226929 |
| incidence of bk virus nephropathy (bkvn) in renal transplant recipients. | bk virus nephropathy (bkvn) is one of the complications of renal transplantation that causes graft loss in renal transplant recipients. | 2012 | 25013634 |
| immunogenicity and other problems associated with the use of biopharmaceuticals. | biopharmaceuticals are used widely for the treatment of cancer, chronic viral hepatitis, inflammatory, and autoimmune diseases. biopharmaceuticals such as interferons are well tolerated for the most part with the most common adverse events observed being 'flu-like' symptoms that resolve rapidly after initial treatment. prolonged treatment is associated, however, with more serious adverse events including leucopenia, thrombocytopenia, and neuropsychiatric effects, which may necessitate dose reduc ... | 2011 | 25083207 |
| binding and internalization of iron oxide nanoparticles targeted to nuclear oncoprotein. | a targeted nanoconjugate is being developed for non-invasive detection of gene expression in cells expressing the jc virus oncoprotein, t-antigen, which has been associated with medulloblastoma and other cancers. jc virus t-antigen localizes predominantly to the nucleus via a classical monopartite nuclear localization signal (nls). an antibody fragment which recognizes jc virus t-antigen was attached to cross-linked dextran coated iron oxide nanoparticles. radiolabeled conjugates were added to m ... | 2010 | 23487404 |
| progressive multifocal leucoencephalopathy: report of two diverse cases. | progressive multifocal leucoencephalopathy (pml) is a rare neurological disorder. the clinical suspicion about this disease in the setting of an immunosuppressive patient even in presence of vague neurological symptoms has to be very high. we describe here two cases of pml a post renal transplant patient and second elderly patient with no underlying immunosuppression. | 2009 | 21475542 |
| progressive multifocal leukoencephalopathy developing in a fludarabine naïve patient with chronic lymphocytic leukemia. | we report a case of progressive multifocal leukoencephalopathy developing in a fludarabine-naïve patient with chronic lymphocytic leukemia. we suggest that progressive multifocal leukoencephalopathy should be considered in any chronic lymphocytic leukemia patient with unexplained neurological symptoms. treatment history does not necessarily require fludarabine. intrathecal cytosine arabinoside although unsuccessful in this patient, is a therapeutic option to consider in patients experiencing sid ... | 2009 | 20360880 |
| metagenomics for the study of viruses in urban sewage as a tool for public health surveillance. | the application of next-generation sequencing (ngs) techniques for the identification of viruses present in urban sewage has not been fully explored. this is partially due to a lack of reliable and sensitive protocols for studying viral diversity and to the highly complex analysis required for ngs data processing. one important step towards this goal is finding methods that can efficiently concentrate viruses from sewage samples. here the application of a virus concentration method based on skim ... | 2018 | 29108696 |
| prevalence, levels and seasonal variations of human enteric viruses in six major rivers in alberta, canada. | the prevalence and seasonal variation of 7 viruses in 6 major rivers in alberta were assessed using a combination of qpcr, cell culture and integrated cell culture with qpcr (icc-qpcr). water samples were collected monthly from rivers at different sites upstream and downstream of major urban centers. seven viruses including rotavirus, adenovirus, astrovirus, norovirus, sapovirus, jc virus and enterovirus, were detected in at least one of the water samples at each site using qpcr. rotavirus was m ... | 2019 | 30743085 |