| alignment of the genome of monkey b-lymphotropic papovavirus to the genomes of simian virus 40 and bk virus. | we located the origin of dna replication of african green monkey b-lymphotropic papovavirus dna by analyzing pulse-labeled form i dna. with the replication origin used as a reference point, the b-lymphotropic papovavirus genome was aligned with the genomes of simian virus 40 and bk virus from dna homology between specific fragments hybridized under low-stringency conditions. from the results of these experiments, it was possible to deduce the correlation between the physical and functional maps ... | 1983 | 6298468 |
| dna encapsidation by viruslike particles assembled in insect cells from the major capsid protein vp1 of b-lymphotropic papovavirus. | capsids of polyomaviruses--small, nonenveloped dna viruses--consist of the major structural protein vp1 and the minor structural proteins vp2 and vp3. the contributions of the individual capsid proteins to functions of the viral particle, such as dna encapsidation, cell receptor attachment, entry, and uncoating, are still not clear. here we show that viruslike particles assembled in nuclei of insect cells from vp1 of the monkey b-lymphotropic papovavirus (lpv) are sufficient to unspecifically en ... | 1996 | 8892870 |
| biochemical studies on structural and nonstructural proteins of the african green monkey b-lymphotropic papovavirus (lpv). | african green monkey (agm) b-lymphotropic papovavirus (lpv) particles were separated from infected b-lymphoma bja-b cells by neuraminidase and deoxycholate treatment and were further purified twice by cscl density centrifugation. the sds-page analysis of virus particles banding at a density of 1.3510 g/ml and radioactively labeled by 125i revealed a major polypeptide of 40,000 and two minor polypeptides of 42,000 and 29,000. in addition, in infected bja-b cells, all three viral structural polype ... | 1983 | 6603052 |
| monkey b-lymphotropic papovavirus dna: nucleotide sequence of the region around the origin of replication. | we have determined the nucleotide sequence of the hindiii-b dna segment of african green monkey b-lymphotropic papovavirus (lpv), which shows a highly restricted host range and whose genome is a 5.1-kilobase-long circular dna. the segment, consisting of 1,123 base pairs, contained the origin of dna replication, the putative control region for early transcription, and the region probably coding for the amino-terminal portion of t antigens. the symmetrical region at the center of replication origi ... | 1984 | 6323745 |
| monkey b-lymphotropic papovavirus mutant capable of replicating in t-lymphoblastoid cells. | monkey b-lymphotropic papovavirus (lpv) dna present as free copies in lpv-transformed hamster embryo cells was molecularly cloned in escherichia coli. twenty-two of 24 cloned dnas were 4.9 kilobases long and shorter than the wild-type lpv dna (5.1 kilobases). the shorter dna was nondefective and generated infectious virus (designated lpv-76) upon transfection of human b-lymphoblastoid bja-b cells. lpv-76 dna had a small deletion in the early region and a deletion and an insertion in the control ... | 1985 | 3925162 |
| monkey b-lymphotropic papovavirus genome: the entire dna sequence and variable regions. | dna from monkey b-lymphotropic papovavirus (lpv) adapted to growth in human b-lymphoblastoid cell line bja-b consisted of three classes of molecules of 5.1, 5.0, and 4.9 kilobases (kb), and the entire dna sequence of 5.1-kb molecule was determined. the three types of lpv dna yielded infectious virus upon transfection to bja-b cells. comparison of the structures among these clones and clone k38 sequenced by pawlita et al. (1985) suggests that the transcriptional control region, the small-t antige ... | 1986 | 3495678 |
| mutation in the vp-1 gene is responsible for the extended host range of a monkey b-lymphotropic papovavirus mutant capable of growing in t-lymphoblastoid cells. | monkey b-lymphotropic papovavirus (lpv) replicates only in b-lymphoblastoid cells, whereas the lpv mutant 76 (lpv-76) can grow in either b- or t-lymphoblastoid cells. the nucleotide sequence of the wild-type lpv psti b segment was compared with that of lpv-76 psti-b, within which the mutation responsible for the extended host range had been located. the vp-1 coding region of lpv-76 was found to have mutations, single-base substitutions causing three amino acid substitutions. | 1986 | 3488416 |