| detection of human papillomavirus dna in skin warts from immunocompromised patients but not in semen from men whose wives have abnormal cervical cytology. | the epidemiology of human papillomaviruses (hpv) was studied in 61 immunocompromised patients (e.g. renal and cardiac transplants; bowen's disease; genital cancer) undergoing therapy at the university hospital of wales at cardiff u.k. warts from various sites of these patients were studied for the presence of hpv types 6, 11, 16 and 18 using the dot-blot dna hybridization technique. four hpv-16 and one hpv-11 was detected. the presence of hpv-16 in our study is quite significant since it suggest ... | 1992 | 1338072 |
| human papilloma virus infection in cervical cytology study. | the prevalence of hpv infection detected by cytology examination in this study (1990) was 2.26 per cent, in which 50.18 per cent of them were hpv infection alone and 49.82 per cent were hpv infection coexisted with abnormal pap smears. this viral infection was detected in all age groups but was highest in the age, between 40 to 44 years, and lowest in the age group older than 65 years. it was also noted that, 25.98 per cent of cin (252 out of 970) and 4.56 percent of carcinoma (19 out of 417) sh ... | 1992 | 1338092 |
| [typing of human papillomavirus (hpv) in the male and female genitalia]. | diagnosis and typing of hpv was started in november 1990 at the virology department of the national health institute, lisbon. eighty one samples from 70 patients were studied, 57 from women observed at the cervico-vulvar out patient unit of the gynecology department of sta maria hospital, lisbon, and 13 from male contacts of some of these women. forty nine women (86%) had colposcopic, histological or cytological evidence of hpv infection. eleven men (85%) have clinical signs of infection and 2 ( ... | 1992 | 1338177 |
| [simultaneous detection by non-isotopic in situ hybridization of human papilloma viruses and epstein-barr virus during the lytic cycle in oral hairy leukoplakia lesions]. | oral hairy leukoplakia is almost only described in patients infected by the human immunodeficiency virus. epstein-barr virus, sometimes associated with human papillomavirus, is always involved in the occurrence of these lesions. we have investigated two cases of oral hairy leukoplakia with the goal of detecting ebv and hpv by using both in situ hybridization and immunohistochemistry. ebv genome was detected with biotinylated bamhi w cdna probe in the two cases. furthermore, ebv was found to be i ... | 1992 | 1338183 |
| non radioactive in situ hybridization for detection of human papilloma virus dna in squamous cell carcinoma of tongue. | previous studies indicate that hpv type 16 and 18 (hpv) are associated with squamous cell carcinoma of the head and neck. in this investigation we evaluate in our hospital 253 patients with oral squamous cell carcinoma of the tongue not related to tobacco or alcohol with a tumor index of t2 no mo between 1981 and 1991. from 12 patients we were able to obtain tissue. for detection of human papilloma virus, dna sequences 6, 11, 16, 18, 31 and 33 in paraffin-embedded human tissue biopsies a non-rad ... | 1992 | 1338425 |
| uptake of exogenous human papilloma virus l1 dna by oocytes and detection by the polymerase chain reaction. | the purpose of this study was to determine if oocytes were capable of taking up exogenous dna such as human papillomaviral (hpv) dna and evaluate the zona pellucida as a barrier to the entry of foreign dna into the oocyte. | 1992 | 1338579 |
| [investigation of infection with human papillomavirus in areas of high and low incidences of cervical carcinoma in sichuan province]. | from jan. to oct. 1989, we investigated the role of various life style and dietary factors in areas of high and low incidences of cervical carcinoma, as well as infection with types 16 and 33 of human papillomavirus (hpv) in populations. the polymerase chain reaction (pcr) technique was used to detect the infection with hpv 16/33. the result showed that the infection rate of hpv was about 17.9% (guangyuan, area of high incidence of cervical cancer), and the infection rate about 3.4% (mian zhu, a ... | 1992 | 1338628 |
| influence of fixation on human papillomavirus dna detection in frozen and embedded paraffin lesions by in situ hybridization with biotinylated probes. | series of frozen or paraffin-embedded tissues from various body sites, taken from non-immunosuppressed or immunosuppressed patients with persistent papilloma lesions were examined for the presence of group specific antigen from human papillomavirus (hpv) by indirect immunofluorescence or hpv dna by in situ hybridization with biotinylated probes. we have shown that it is possible to detect hpv dna after fixation of tissues in neutral formalin, bouin's or baker's solution. however, the sensitivity ... | 1992 | 1338657 |
| prognostic value of human papillomavirus in the survival of cervical cancer patients: an overview of the evidence. | some studies have suggested that the presence in tumors of nucleic acids from human papillomavirus (hpv) constitutes a prognostic marker of disease severity in cervical cancer. there are two conflicting lines of evidence in this regard. first, the presence of hpv 18 is equated to rapid progression through early disease stages, possibly resulting in a more aggressive clinical course. although fragmentary, in terms of the clinical and epidemiological basis, this line of evidence has some experimen ... | 1992 | 1338870 |
| the association of human papillomavirus antibodies with cervical cancer risk. | the association between viral human papillomavirus (hpv) dna and cervical carcinoma has been well documented. however, less is known about the immune response to hpv infections and its relationship to cervical cancer risk. a higher prevalence of antibodies to hpv16 e7 among women with cervical cancer compared with controls has been reported, but reactivity to other antigens has not been systematically examined. prevalence of serum igg antibody reactivities to hpv6-encoded l1 and l2 and to hpv16- ... | 1992 | 1338895 |
| detection of human papillomavirus dna in normal epithelium and in squamous metaplasia of the uterine cervix by the polymerase chain reaction. | the presence of human papillomavirus (hpv) 16 was studied by using the polymerase chain reaction (pcr) directly on sections histologically defined as normal squamous epithelium and metaplastic squamous tissue of the uterine cervix. ten specimens of normal epithelium were obtained at hysterectomy from women with uterine leiomyoma. six specimens of squamous metaplasia were adjacent to the areas of dysplastic epithelium in which hpv 16 dna had been detected previously by pcr. hpv 16 dna was amplifi ... | 1992 | 1339001 |
| detection and analysis of human papillomavirus type 1 infection of skin warts from a dermatology clinic in taiwan by southern hybridization. | thirty cases of skin warts from the dermatology clinic at tri-service general hospital were analyzed for the presence of human papillomavirus type 1 sequences by southern blot hybridization. thirteen of the 30 cases were hpv-1 positive. the prevalence was 43%. episomal hpv-1 sequences were detected in 11 of 13 hpv-1 positive cases. there are two cases which probably contained integrated forms, one contained the higher molecular weight bands, and the other with 6 kb viral genome may be the result ... | 1992 | 1339087 |
| low p53 level in immortal, non-tumorigenic oral keratinocytes harboring hpv-16 dna. | the p53 protein level was determined in normal oral keratinocytes and two non-tumorigenic, immortal oral keratinocyte lines harboring human papillomavirus-16 (hpv-16)dna. the p53 mrna level in the immortal cells was higher than the normal counterpart, but the p53 protein level was notably lower in the immortalised cells. the half-life of p53 protein in the normal and immortal cells was < 1 h, and the p53 cdna sequence of these cells showed no mutation. the immortal cells transcribed a high amoun ... | 1992 | 1339128 |
| in vitro and animal studies of the role of viruses in oral carcinogenesis. | the linkage of herpes simplex virus (hsv) and human papillomavirus (hpv) to the development of oral cancer has been studied. in spite of the presence of viral nucleic acids in some human oral cancer specimens, hsv alone is not carcinogenic in animals: repeated viral inoculation to mouse or hamster oral mucosa fails to produce tumours or histopathological evidence of malignancy. however, hsv demonstrates co-carcinogenicity in vivo: viral inoculation significantly enhances the oncogenic capacity o ... | 1992 | 1339129 |
| viruses in anogenital cancer. | the association between sexual activity and cancer, first described in carcinoma of the cervix, has been expanded to include the majority of anogenital squamous epithelial carcinomas. current evidence suggests that human papillomavirus (hpv) may be of great importance in the development of these tumours, whilst herpes simplex type 2 virus (hsv-2) and human immunodeficiency virus (hiv) may play minor roles. certain types of hpv dna, including types 16, 18, 31, 33 and 39 are found in most but not ... | 1992 | 1339181 |
| [early anti-hpv 16 protein antibodies in patients with anogenital tumors]. | a study was worked out in female patients with anogenital tumors and in apparently healthy women, aimed at the detection of antibodies against e4 and e7 hpv 16 proteins. as regards the detection of hpv 16 infection, the dna hybridization and western-blot results were in good agreement. the hpv 16 infection was detected in a relatively high number of the patients (17 out of 24) and of the apparently healthy women using western blot. | 1992 | 1339204 |
| [detection of human papillomavirus (h.p.v.) dna in genital lesions using molecular hybridization]. | detection of human papilloma virus in genitals lesions by molecular hybridization. some h.p.v. types are sexually transmitted and infect genital organs. we have used molecular hybridization to examine the distribution of h.p.v. 6 or ii and h.p.v. 16 in benign, premalignant and malignant genital lesions from 344 patients. the frequency of h.p.v. 16 positive cases increases as the cervical lesions progress to malignancy: 57/78 are positive (73%) in the carcinomas, 29/83 are positive (35%) in mild ... | 1992 | 1339249 |
| inhibition of the transformed phenotype of carcinoma cells that contain human papillomavirus. | | 1992 | 1340137 |
| antisense oligonucleotides as antiviral agents. | | 1992 | 1340153 |
| papillomaviruses in human disease: part i. pathogenesis and epidemiology of human papillomavirus infections. | papillomas of man and animals were shown to contain an infectious agent at the beginning of this century. since then, various attempts were made to isolate the papilloma inducing agent. electron microscopic studies later revealed that the agent is a virus, however all attempts to culture it with conventional virological techniques failed. it took until the development of molecular cloning techniques before a detailed study of this virus could start. today it is clear that the papilloma-associate ... | 1992 | 1341482 |
| human papillomavirus detection in cervical dysplasias or neoplasias and in condylomata acuminata by in situ hybridization with biotinylated dna probes. | specimens from cervical dysplasias or carcinomas and genital condylomata acuminata were retrospectively analysed by in situ hybridization (ish) with biotinylated dna probes for human papillomavirus (hpv) types 6, 11, 16 and 18. in the control group no case was positive for hpv dna. in mild/moderate dysplasias, 4 cases (14%) were positive for hpv 6 or 11 and 2 cases (7%), for hpv 16. in the severe dysplasia/in situ carcinoma group, 9 cases (31%) showed presence of dna of hpv types 16 or 18. six i ... | 1992 | 1342087 |
| hpv dna in intraepithelial neoplasia and carcinoma of the vulva and penis. | surgical specimens of 15 patients with early and 12 patients with advanced squamous cell carcinoma of the vulva and the penis were examined for the presence of human papillomavirus (hpv) type 6, 11, 16, and 18 dna by southern blotting (sb) and polymerase chain reaction (pcr) analysis. by sb, hpv type 16 dna was detected in all early carcinomas and 2 of 12 cases of advanced squamous cell carcinoma (iscc) of the vulva and penis. pcr revealed hpv dna in four additional cases of vulvar and penile is ... | 1992 | 1342951 |
| dna content measurement and in situ hybridization in condylomatous cervical lesions. | the present study reports results obtained with dna ploidy measurement by feulgen cytophotometry and in situ hybridization (ish) on the same sections of 36 cervical lesions containing human papillomavirus (hpv) dna. fifteen of 16 low grade lesions [11 flat condylomas and five condylomatous with intraepithelial neoplasias (cin)-1, 1 with condylomatous features] were diploid. "oncogenic" hpv (types 16 and 33) were detected in three of these cases, while the aneuploid case expressed hpv type 11. by ... | 1992 | 1342964 |
| a new nonisotopic detection of human papillomavirus dna using polymerase chain reaction. | a novel technique using a two-step polymerase chain reaction (pcr) with specific primers detecting human papillomavirus (hpv) dna of types 6/11, 16, and 18 and a final nonisotopic colorimetric detection has been developed. sixty formalin-fixed and paraffin-embedded sections were treated with this methodology and the results compared with those obtained with in situ hybridization (ish). twenty cases displaying hpv dna with ish were positive with pcr. seven (35%) of 20 cases negative for ish but e ... | 1992 | 1342972 |
| human papillomavirus and anogenital cancers in northern brazil. | | 1992 | 1343656 |
| human papillomavirus, human immunodeficiency virus, and cervical cancer: newly recognized associations? | there are now sufficient data to conclude that women infected with human immunodeficiency virus (hiv) have an increased risk of human papillomavirus (hpv) infection and preinvasive stages of cervical cancer. this association is not completely due to immunosuppression. it is likely that hpv pathogenesis is altered in hiv-infected women. preinvasive cervical neoplasia likely occurs more frequently in hiv-infected women because of several factors, including immunosuppression, viral interactions, an ... | 1992 | 1344670 |
| leukoregulin and gamma-interferon inhibit human papillomavirus type 16 gene transcription in human papillomavirus-immortalized human cervical cells. | the human papillomavirus (hpv) transforming genes e6 and e7 are retained and expressed in the majority of cervical cancers implying an important role for these proteins in maintenance of the malignant phenotype. leukoregulin (lr) and recombinant gamma-interferon (r-ifn-gamma), lymphokines secreted by immune cells present in regressing hpv infections, inhibited transcription of e6/e7 rnas in several human cervical epithelial cell lines immortalized by recombinant hpv-16, -18, and -33 dnas. r-ifn ... | 1992 | 1345813 |
| detection of human papillomavirus types 16 and 18 in the exfoliated cervical cells using the polymerase chain reaction. | we applied the polymerase chain reaction (pcr) to detect hpv 16 and 18 in cytological samples obtained from the uterine cervices of japanese women. hpv infection was detected in 17 (25%) of 67 with cin and 11 (37%) of 30 with cervical carcinoma. it is notable that 11 (16%) of 69 women with normal cervices were infected with either hpv 16 or 18. the polymerase chain reaction is sensitive and useful for epidemiological studies. | 1992 | 1346595 |
| hpv-16-related dna sequences in kaposi's sarcoma. | in the usa, kaposi's sarcoma associated with the acquired immunodeficiency syndrome (aids-ks) is ten times more common in homosexual or bisexual men than in heterosexual men with aids. one explanation for this finding is that aids-ks may be caused by an infectious agent. because there is a high incidence of human papillomavirus (hpv) infection, especially hpv-16, in homosexual men, we have sought hpv dna sequences in kaposi's sarcoma. we used the polymerase chain reaction with a primer pair spec ... | 1992 | 1346878 |
| antiviral agents. | in recent years, the antiviral armamentarium has expanded considerably. currently available agents are virustatic, inhibiting specific steps in the process of viral replication. no agent is active against nonreplicating or latent viruses. acyclovir is useful in the treatment of genital herpes, herpes simplex encephalitis, mucocutaneous herpetic infection, varicella infection in the immunosuppressed host, and herpes zoster infection in the normal and the immunosuppressed host. it can also be used ... | 1992 | 1347578 |
| human papillomavirus type 16 and kaposi's sarcoma. | | 1992 | 1348340 |
| human papillomavirus type 16 and kaposi's sarcoma. | | 1992 | 1348342 |
| human papillomavirus type 16 in cervical smears as predictor of high-grade cervical intraepithelial neoplasia [corrected]. | the management of women with mild to moderately dyskaryotic cervical smears would benefit from a non-invasive test that predicts which women have high-grade cervical intraepithelial neoplasia. detection of human papillomavirus type 16 (hpv16) dna in cervical smears may be such a test. with the polymerase chain reaction (pcr), we estimated the amount of hpv16 dna in cervical smears from 85 women referred for colposcopy because of abnormal cytology. an intermediate or high amount of hpv16 dna pred ... | 1992 | 1348800 |
| clonal p53 mutation in primary cervical cancer: association with human-papillomavirus-negative tumours. | analyses of cancer cell lines and of anal cancers suggest an inverse correlation between infection with human papillomavirus (hpv) and somatic mutation of the p53 tumour-suppressor gene. we have investigated this association in primary cervical tumours. tumour-tissue samples from 28 women with primary cancer of the cervix were analysed for presence of hpv sequences and for somatic mutations of the p53 gene. southern blot analysis and the polymerase chain reaction (pcr) showed that 25 of the tumo ... | 1992 | 1349102 |
| human papillomavirus (hpv) type 16 in semen of partners of women with hpv infection. | | 1992 | 1349130 |
| relationship of human papillomavirus type to grade of cervical intraepithelial neoplasia. | objective--to determine the relationship of human papillomavirus (hpv) type to grade of cervical intraepithelial neoplasia (cin) in a large series of cases. design--a survey of hpv types in cin lesions detected using a new, highly accurate method for typing hpv that is based on restriction fragment length polymorphism analysis of amplimers produced during polymerase chain amplification of the conserved l1 region of hpv using consensus primers. setting--private gynecologists' offices and inner-ci ... | 1992 | 1349360 |
| [detection of human papillomavirus (hpv) dna in carcinoma of uterine cervix and genital tract diseases in jiangsu province, china]. | pst-1 cleaved dna restriction fragment length polymorphism (rflp) of biopsy samples from 41 patients with squamous cell carcinoma of uterine cervix collected in jiangsu province, china were examined for hpvs by southern blot hybridization using hpv 16 dna as a probe. 20 of the 41 samples were positive for hpvs when hybridized under non-stringent condition. hpv was not detectable in samples collected in the same time period from patients with cervical adenocarcinoma (n = 2), vaginal carcinoma (n ... | 1992 | 1349522 |
| vaginal microbial flora as a cofactor in the pathogenesis of uterine cervical intraepithelial neoplasia. | the vaginal microbial flora of 106 women with histopathologically confirmed cervical intraepithelial neoplasia and 79 women without disease, was evaluated for gardnerella vaginalis, trichomonas vaginalis, candida albicans and other yeasts. flora morphology was assessed by gram staining of secretions. cervical cultures were examined for herpes simplex virus, cytomegalovirus and neisseria gonorrhoeae. chlamydia trachomatis antigens in cervical secretions were detected by enzyme immunoassay. human ... | 1992 | 1351005 |
| mild cervical dyskaryosis: safety of cytological surveillance. | how best to manage women who are found on cervical screening to have mild dyskaryosis remains controversial. cytological surveillance misses some lesions picked up by colposcopy, but colposcopy is emotionally traumatic for women, the majority of whom will have a normal result. to determine what proportion of lesions are missed by cytological surveillance, and whether any abnormalities persist after colposcopy, we studied, by means of colposcopy and biopsy, the prevalence of cervical intraepithel ... | 1992 | 1351129 |
| failure to detect human papillomavirus in kaposi's sarcoma. | | 1992 | 1351572 |
| langerhans cells, inflammation markers and human papillomavirus infections in benign and malignant epithelial tumors from transplant recipients. | organ transplant recipients frequently develop warts which progress toward premalignant or malignant lesions after a rather long grafting period. the local immune responses of such lesions (warts, condyloma acuminata, actinic keratoses, bowen, basal and squamous cell carcinomas) was studied in 32 frozen skin specimens taken from 15 male transplant recipients and compared to similar lesions from the normal population. we studied the expression of t cell subsets, langerhans cell phenotype, hla cla ... | 1992 | 1352312 |
| pcna expression in cutaneous keratinous neoplasms and verruca vulgaris. | using an antibody to pcna and a standard immunohistochemical system, the authors examined normal epidermis and cutaneous neoplasias for expression of pcna, a protein associated with dna polymerase delta and dna replication. in squamous cell carcinoma in situ (scci), a unique expression of pcna, which frequently involved the nuclei of all keratinocytes within the lesion, was found. heaviest staining was in the uppermost layers of the epidermis. pcna expression ended abruptly at the histologic mar ... | 1992 | 1352943 |
| detection of human papillomavirus types by the polymerase chain reaction and the differentiation between high-risk and low-risk cervical lesions. | by means of a consensus polymerase chain reaction (pcr) method, the prevalence of hpv types was determined in cervical biopsies from 137 women referred to the gynecological outpatient clinic for colposcopy because of an abnormal cervical smear. the prevalence of hpv was 80.3%. there was a statistically highly significant rise in the prevalence of the oncogenic hpv types (16, 18, 31, 33) with increasing severity of cervical intraepithelial neoplasia (cin i to iii), indicating a role for these hpv ... | 1992 | 1357817 |
| anal intraepithelial neoplasia: part of a multifocal disease process. | invasive carcinomas of the anogenital epithelium share a common aetiological factor--human papillomavirus (hpv) type 16. although genital intraepithelial neoplasia may be multifocal, there have been no studies of the prevalence of anal intraepithelial neoplasia in women with intraepithelial neoplasia of the genital tract. we tested the hypothesis that women with high-grade cervical intraepithelial neoplasia are at higher risk of disease in the anus than are control women of similar age with no h ... | 1992 | 1359331 |
| genetic lesion in the carcinogenesis of cervical cancer. | allelic loss at specific chromosomal loci was demonstrated in carcinoma of the cervix (cacx) obtained from hong kong chinese by restriction fragment length polymorphism analysis. altogether 12 patients with cacx, 7 with cervical intraepithelial neoplasia (cin) and 7 with normal cervical tissues were studied. polymorphic dna markers for chromosome 3 were used and the tumour and constitutional genotypes of the above patients were compared. loss of heterozygosity (loh) was found in 10 out of 12 inf ... | 1992 | 1359830 |
| human papillomavirus dna in urine of women with preneoplastic and neoplastic cervical lesions. | | 1992 | 1360129 |
| rearrangements of the upstream regulatory region of human papillomavirus type 6 can be found in both buschke-löwenstein tumours and in condylomata acuminata. | clinically malignant buschke-löwenstein tumours and benign condylomata acuminata are caused by human papillomaviruses (hpvs), predominantly hpv-6 and -11. in some cases, the hpv-6 genomes found in buschke-löwenstein tumours and in verrucous carcinomas differ from hpv-6b isolated from a benign genital wart, by rearrangements of the upstream regulatory region (urr). to evaluate the frequency and role of mutations of the urr of hpv-6 we analysed 42 condylomata acuminata and four buschke-löwenstein ... | 1992 | 1361521 |
| epidermotropism of t cells correlates with intercellular adhesion molecule (icam1) expression in human papillomavirus (hpv)-induced lesions. | adhesion molecules play an important role in inflammatory reactions. among them, icam1, a ligand for the lymphocyte function-associated antigen (fla1) of leucocytes, may be expressed by antigen-presenting cells and keratinocytes in various inflammatory disorders. as cell-mediated immune responses play a great role in hpv infections, we investigated the expression of icam1 and correlated it with the presence of lfa1-positive cells by immunohistochemistry on serial frozen sections of a series of n ... | 1992 | 1361527 |
| therapeutic effect of carbon dioxide laser versus single application of trichloroacetic acid for koilocytic squamous papillae. | this paper reports the treatment results of 50 symptomatic females who had clinical features of squamous papillae and histologic evidence of koilocytosis. either a carbon dioxide laser or a single application of trichloroacetic acid (tca) was used to treat these patients. all of the patients were alternately and prospectively randomized into one of two groups. twenty-three of the 25 women treated by laser and 10 of the 25 women treated by tca had resolution of either the symptoms or the vulvar l ... | 1992 | 1363204 |
| urological evaluation of sexual partners of women with human papillomavirus (hpv) infection. | cancer of the uterine cervix has been related to hpv infection, based on clinical and laboratory data. the high recurrence rate in couples undergoing treatment for hpv infection points to a probable viral reservoir, either in subclinical lesions or in male internal genital organs. we have evaluated 31 men, all sexual partners of women with hpv infection. eleven patients (35.5%) had related lesions: 4 (12.9%) with condyloma acuminatum; 5 (16.1%) with lesions revealed by magnified examination afte ... | 1992 | 1369626 |
| increased sensitivity of human keratinocytes immortalized by human papillomavirus type 16 dna to growth control by retinoids. | human papillomavirus (hpv) type 16 (hpv16) is associated with a large percentage of cervical malignancies, and hpv16 dna can immortalize human keratinocytes in vitro. the transforming ability of the virus resides primarily in the open reading frames e6 and e7. retinoids are potent modulators of growth and differentiation of keratinocytes and have been shown to reverse cervical lesions resulting from hpv infection. we compared the sensitivity of normal human foreskin keratinocytes (hkc) and four ... | 1992 | 1370074 |
| altered expression of proliferation and differentiation markers in human papillomavirus 16 and 18 immortalized epithelial cells grown in organotypic culture. | the patterns of expression of keratins k1 and k8, filaggrin, and the proliferation-associated protein, proliferating cell nuclear antigen (pcna), were studied in normal and human papillomavirus (hpv) 16 or 18 immortalized keratinocyte cell lines grown on organotypic raft cultures. normal keratinocytes produced an epithelial sheet that closely resembled epidermis in vivo, characterized by lack of k8 expression, pcna expression restricted to the basal layer, and k1 and filaggrin expression in the ... | 1992 | 1370596 |
| epitope-mapped monoclonal antibodies against the hpv16e1--e4 protein. | the human papillomavirus (hpv) e1--e4 protein is the only nonstructural late protein encoded by the virus. we have isolated three hybridomas producing monoclonal antibodies to the e1--e4 protein of hpv16, which is the hpv type most frequently associated with cervical cancer. the three antibodies (tvg 401, 402, and 403) detect adjacent epitopes within the major seroreactive region of the molecule and show no reactivity against the e4 proteins of hpv1, hpv2, hpv4, or hpv6. the e1--e4 protein migra ... | 1992 | 1371027 |
| epitope mapping of the human papillomavirus type 16 e4 protein by means of synthetic peptides. | eight overlapping icosapeptides covering the entire sequence of the e4 protein of human papillomavirus type 16 (hpv-16), were prepared and tested for their reactivity with human sera in igg-specific elisa. the strongest reactivity of sera from hpv-16 dna-positive invasive cervical carcinoma (inca) patients was detected with the peptide denoted 16/e4-6, covering amino acids 51 to 70. subsequently nearly 200 sera were tested for the presence of the 16/e4-6-specific antibody. reactivity was more fr ... | 1992 | 1371544 |
| superficial laser vulvectomy. v. surgical debulking is enhanced by adjuvant systemic interferon. | skillful laser ablation can remove any volume of human papillomavirus-associated vulvar disease but cannot prevent reactivation of the surrounding latent viral reservoir during postoperative healing. conversely, interferon and 5-fluorouracil are relatively ineffective as primary therapies in clearing bulky lesions. in this study of 71 assessable patients, topical 5-fluorouracil and systemic interferon injections were used postoperatively. success rates within the adjuvant 5-fluorouracil and lase ... | 1992 | 1372470 |
| [cancer of the penis and its treatment]. | it had been believed that carcinoma of the penis was rather rare in the developed countries comparing with that in the under-developing countries, however, the recent epidemiological studies failed to reveal any clear difference of the incidence of carcinoma of the penis all over the world. in these days so called successful treatment is coming to be evaluated by the quality of life (qol) after surgical or nonsurgical treatment (especially sexual function tended to be considered very important f ... | 1992 | 1373451 |
| desmoplastic trichilemmoma. | seven cases of desmoplastic trichilemmoma (dt), a recently described pseudomalignant variant of trichilemmoma, are reviewed. the tumor generally occurs in men after the fifth decade of life and presents as a small solitary nodule on the face. it is frequently misdiagnosed clinically as a basal cell carcinoma or a papilloma. histologically dt displays a superficial lobular growth arranged about a central prominent desmoplastic stroma. at the periphery, the tumor lobules show the typical features ... | 1992 | 1373583 |
| abnormalities of epidermal differentiation associated with expression of the human papillomavirus type 1 early region in transgenic mice. | the promoter region of a keratin 6 (k6) gene was used to regulate expression of the early region of human papillomavirus type 1 (hpv-1e) in transgenic mice. in one line of mice the k6-hpv1e transgene was transcribed in several regions of the skin, the predominant transcript being a 1.1 kb rna including the e4 open reading frame, and e1-e4 protein was detected in the upper suprabasal layers of the skin in paws and tail. a 1.7 kb rna corresponding to the e6/e7 transcript was also prominent in tail ... | 1992 | 1375281 |
| definition of immunogenic determinants of the human papillomavirus type 16 nucleoprotein e7. | specific t lymphocyte lines and t cell clones were established from peripheral blood mononuclear cells of asymptomatic seropositive individuals employing synthetic peptides which correspond to the sequence of the human papillomavirus (hpv) type 16 transforming protein e7. specificity analysis of t cells as determined by means of [3h] thymidine incorporation after stimulation with individual peptides revealed three immunogenic determinants of e7 that are recognised in association with at least tw ... | 1992 | 1375481 |
| hla class ii antigen expression in human papillomavirus-associated cervical cancer. | the observation that tumor cells of some neoplasms display major histocompatibility complex (mhc) class ii molecules may be of functional significance, influencing the progression of malignancy by allowing the cancer cells to present antigen to the immune system. in the normal cervix, class ii molecules are expressed by columnar but not squamous epithelium. the pattern of mhc class ii expression in cervical carcinomas has been documented using immunohistochemical methods. of 53 cervical squamous ... | 1992 | 1377602 |
| levels of immunoglobulin g antibodies against defined epitopes of the l1 and l2 capsid proteins of human papillomavirus type 6 are elevated in men with a history of condylomata acuminata. | sera from 159 men attending the sexually transmitted disease clinic at karolinska hospital, stockholm, sweden, were analyzed for the presence of immunoglobulin a (iga) and igg antibodies to a panel of synthetic peptides derived from the e2, l1, and l2 regions of the human papillomavirus types 1 (hpv 1), 6, 8, 11, 16, 18, 31, and 33. the study subjects were divided into three groups: (i) asymptomatic men with no history of genital warts who served as controls, (ii) men with visible condylomata, a ... | 1992 | 1378454 |
| squamous metaplasia of normal and carcinoma in situ of hpv 16-immortalized human endocervical cells. | the importance of cervical squamous metaplasia and human papillomavirus 16 (hpv 16) infection for cervical carcinoma has been well established. nearly 87% of the intraepithelial neoplasia of the cervix occur in the transformation zone, which is composed of squamous metaplastic cells with unclear origin. hpv dna, mostly hpv 16, has been found in 90% of cervical carcinomas, but only limited experimental data are available to discern the role of hpv 16 in this tissue specific oncogenesis. we have i ... | 1992 | 1379123 |
| human papillomavirus e6 proteins bind p53 in vivo and abrogate p53-mediated repression of transcription. | the transforming proteins of dna tumor viruses sv40, adenovirus and human papillomaviruses (hpv) bind the retinoblastoma and p53 cell cycle regulatory proteins. while the binding of sv40 large t antigen and the adenovirus e1b 55 kda protein results in the stabilization of the p53 protein, the binding of hpv16 and 18 e6 results in enhanced degradation in vitro. to explore the effect of viral proteins on p53 stability in vivo, we have examined cell lines immortalized in tissue culture by hpv18 e6 ... | 1992 | 1379175 |
| study of infection by human papillomavirus in severe dysplasias and carcinomas in situ of the uterine cervix using immunohistochemistry and in situ hybridization. | diagnosis of human papillomavirus (hpv) infection in uterine cervical lesions is usually based on histopathological criteria and, in some cases, is confirmed by immunohistochemistry. the recent development of in situ hybridization techniques has facilitated the detection of hpv in these lesions. consequently, we carried out a study on 18 uterine cervical biopsy specimens histopathologically diagnosed as severe dysplasias and carcinomas in situ, using an immunohistochemical method with a rabbit p ... | 1992 | 1380847 |
| induction of cytotoxic t lymphocytes with peptides in vitro: identification of candidate t-cell epitopes in human papilloma virus. | a set of overlapping peptides corresponding to the l1, e6, and e7 proteins of human papilloma virus 16 was tested for their ability to bind to major histocompatibility complex class i molecules and to stimulate cytotoxic t-lymphocyte (ctl) responses in vitro. a class i binding assay using intact rma-s cells showed that 20 of the 99 human papilloma virus peptides bound to h-2kb and/or db molecules. fifteen of the 20 class i-binding peptides stimulated primary ctl responses, whereas peptides that ... | 1992 | 1381502 |
| viral integration and fragile sites in human papillomavirus-immortalized human keratinocyte cell lines. | human papillomavirus (hpv) types 16 and 18 integration sites were mapped in six hpv-immortalized human keratinocyte cell lines by fluorescence in situ hybridization (fish). mapping of hpv sequences in these cell lines revealed that hpv integration varied in copy number and location but that integration sites were stable over extended passages in culture. integration occurred at different sites throughout the genome and did not correspond to the location of specific cellular genes. however, integ ... | 1992 | 1381951 |
| hpv-1 l1 protein expressed in cos cells displays conformational epitopes found on intact virions. | seven polyclonal and monoclonal antibodies were characterized for their ability to react specifically with either conformational or nonconformational epitopes of the hpv-1 virion. using these antibodies, it was shown that the hpv-1 l1 protein (when expressed by an sv40 vector in cos cells) displayed conformational epitopes characteristic of intact viral particles. in addition, the l1 capsid protein was translocated normally into cell nuclei, was of appropriate size (57 kda), and could be isolate ... | 1992 | 1382345 |
| down-regulation of keratin 14 gene expression after v-ha-ras transfection of human papillomavirus-immortalized human cervical epithelial cells. | keratin expression in human cervical squamous cell carcinoma (scc) lines differed significantly from both normal and human papillomavirus (hpv) immortalized exocervical cells. keratin 14 (k14) expression, determined by protein synthesis and mrna levels, was dramatically down-regulated in the cervical scc lines while keratin 5 (k5) expression was not. k14 expression was similarly down-regulated in an hpv-16 immortalized cervical cell line after tumorigenic transformation with recombinant v-ha-ras ... | 1992 | 1382846 |
| presence of ras oncogene mutations and human papillomavirus dna in human prostate carcinomas. | the frequency of h-ras, k-ras, and n-ras mutations and the presence of high-risk human papillomavirus (hpv 16, 18, and 33) dna were studied in 75 paraffin-embedded specimens obtained from 68 japanese patients with a variety of prostate carcinomas by using polymerase chain reaction and dna hybridization with sequence-specific oligonucleotides. ten specimens each of normal and benign hyperplastic prostatic tissues from the same number of patients were also examined for this analysis. of 68 carcino ... | 1992 | 1382850 |
| patterns of keratin 19 expression in normal, metaplastic, condylomatous, atrophic, dysplastic, and malignant cervical squamous epithelium. | keratin 19 (k-19) expression has been strongly correlated with dysplasia in oral epithelium. expression of k-19 was evaluated by immunoperoxidase staining in formalin-fixed normal ectocervical tissue, normal endocervical tissue, cervical dysplasia, squamous metaplasia, atrophic epithelium, cervical condylomas, and invasive carcinoma to determine if a correlation of k-19 expression with dysplasia was present in the cervical epithelium. uniform expression of k-19 was seen in endocervical epitheliu ... | 1992 | 1384306 |
| preferential chromosome loss in human papilloma virus dna-immortalized mammary epithelial cells. | human papilloma virus (hpv) dna-immortalized human mammary epithelial cells may provide a model system for studying the molecular basis of immortalization and its role in breast neoplasia. cytogenetic analyses were performed on clones derived from hpv 16- and hpv 18-immortalized human mammary epithelial cells. the majority of the clones contained near-diploid karyotypes. the single most frequent whole-chromosome loss was that of chromosome 19. regions that showed preference for deletion and/or t ... | 1992 | 1384674 |
| therapy of genital human papillomavirus infections. part ii: methods of treatment. | | 1992 | 1385343 |
| women in the hiv epidemic. | in the united states, the majority of women with hiv/aids are women whose lives have been touched by injection drug use--their own or that of their sexual partners. the pattern of opportunistic infections in women is more similar to that of injection drug users than that of homosexual men. specific complications associated with a woman's reproductive tract include persistent candida vaginitis, human papillomavirus infections, cervical dysplasias, and, possibly, pelvic inflammatory disease. child ... | 1992 | 1388989 |
| tumour-infiltrating lymphocytes in cervical carcinoma. | tumour-infiltrating lymphocytes from cervical carcinomas were cultivated in the presence of interleukin-2. the majority of bulk cultures were cytotoxic against k562, mel 1 and caski cells. cd8+ cells were the predominant subset in over 50% of cultures, with varying numbers of cd56+ and cd25+ cells. t cell clones were established from six tumour-infiltrating lymphocyte cultures and the majority exhibited non-mhc-restricted cytotoxicity. however, in one case cytotoxicity of several derived clones ... | 1992 | 1389534 |
| [intra-epithelial neoplasia of the vulva and smoking]. | vin iii etiology is multifactorial with a predominant role held by human papillomavirus infections, especially infections with hpv type 16. other cofactors are also involved. we reviewed our patients presenting with vin iii, focusing our attention on smoking. out of 37 patients 29 (78%) were smokers and among those who presented with a relapse after treatment (11 patients) all were smokers. we discuss mechanisms by which tobacco could act as a cofactor in vin iii. | 1992 | 1392634 |
| association between malignancies of the upper aerodigestive tract and uterine cervix. | cancers of the cervix and buccal cavity share histologic, epidemiologic, and exposure characteristics. in particular, cigarette smoking and human papillomavirus (hpv) have been cited as etiologic cofactors of both malignancies. using incidence data from the surveillance, epidemiology, and end results (seer) program of the national cancer institute for the years 1973 through 1984, we evaluated the incidence of second cancers of the buccal cavity following an initial cervical cancer. standardized ... | 1992 | 1399566 |
| triple cancers in the urogenital area of a patient with aplastic anemia. | three epithelial neoplastic lesions, perineal bowenoid papulosis, uterine cervical carcinoma, and bladder transitional cell carcinoma, which occurred in a mildly immunosuppressed patient who had aplastic anemia were studied for human papillomavirus (hpv) infection. in the bowenoid papulosis, hpv type 16 dna was identified by polymerase chain reaction (pcr) and by in situ hybridization (ish). in contrast, in the uterine cervical carcinoma, hpv 16 was not detected, although possibly another uniden ... | 1992 | 1401490 |
| giant condyloma acuminatum in a baby boy. | a giant condyloma acuminatum developed on the penis of a one and a half-year-old japanese boy in two months. the histological features of this tumor were compatible with those of ordinary condyloma acuminatum. although we detected the presence of human papilloma virus (hpv) type 16 by using the polymerase chain reaction system, we could not rule out the possibility that this hpv was present concurrently with other hpv types that cause condyloma acuminatum. the lesion was successfully treated wit ... | 1992 | 1401502 |
| [tumor suppressor genes: mutations in rb and p53 genes are significant carcinogenic factors]. | the rb and p53 tumour suppressor genes encode nuclear proteins that exert an inhibitive effect on cell growth. a large variety of human tumour types manifest loss or mutation of the rb or p53 genes, and p53 mutation is the commonest genetic alteration found in tumour cells. in addition, the rb and p53 proteins may be inactivated by complex formation with viral oncoproteins--for instance, in the case of cervical carcinoma carrying human papilloma virus. in vivo introduction of an intact rb or p53 ... | 1992 | 1405928 |
| issues concerning women and aids: sexuality. | in the us and globally women are contracting the human immunodeficiency virus (hiv) and developing the acquired immunodeficiency syndrome (aids) the fastest. worldwide, hiv is transmitted primarily through heterosexual intercourse. in the us, the proportion of women who have contracted aids by heterosexual transmission has increased from 11% in 1984 to 34% in 1990. women are at a greater risk than men for transmission by heterosexual intercourse as the ratio of women to men who acquire aids b ... | 1992 | 1408853 |
| multiple partners and partner choice as risk factors for sexually transmitted disease among female college students. | multiple sexual partners and partner choice are believed to increase the risk of sexually transmitted disease (std), but these behaviors had not previously been assessed outside of clinical populations. in this study, a cross-sectional survey among single, white, female students in their senior year of college was conducted to measure the association between behavioral risk factors and the acquisition of self-reported stds during college. the usable response rate was 47.2% (n = 467). the combine ... | 1992 | 1411843 |
| adenovirus transformation revertant resistant to retransformation by e1 but not by sv40-t and hpv16-e7 oncogenes. | we have previously described a revertant cell line which expresses a dominant tumor suppressor phenotype to e1 but not to heterologous oncogenes such as c-myc, n-ras, or polyoma middle t (sircar et al. (1988) oncogene 3, 725-728). dna tumor virus oncogenes have been suggested to transform cells via the common mechanism of sequestering the rb-105 antioncoprotein. this paradigm would predict that our revertant cell line, which is resistant to retransformation by e1a, should also be resistant to th ... | 1992 | 1413500 |
| significance of atypical cervical cytology in pregnancy. | to ascertain the significance of squamous atypia encountered during routine papanicolaou smear screening in pregnancy, we reviewed our experience with 76 pregnant women seen during a 4-year period. all were evaluated with repeat cytology and colposcopy during pregnancy and again postpartum. colposcopic examination during pregnancy revealed a normal transformation zone without evidence of intraepithelial neoplasia in 46 women. in six of these women, repeat cytology was interpreted as cervical int ... | 1992 | 1418129 |
| induction of transformation of human respiratory epithelium in vitro. preliminary investigation. | malignancy is the result of multistep transformational changes of normal somatic cells. in the case of respiratory epithelial malignancies this process lasts for several years. many methods have been explored to mimic this process in an extracorporal model. in the present investigation we combined several of these methods. organ cultures were prepared from tracheal specimens and were then consecutively treated with human papilloma virus, benzo(a)pyrene, methylnitronitrosoguanine and tetradecanoy ... | 1992 | 1418947 |
| specific phosphorylation of the acidic central region of the n-myc protein by casein kinase ii. | the central region of the n-myc protein has a characteristic amino acid sequence edtlsdsddedd, which is very similar to those of particular domains of adenovirus e1a, human papilloma virus e7, simian virus 40 large t, c-myc and l-myc proteins. domains of these three viral oncoproteins have recently been shown to be specific binding sites for the tumor-suppressor gene retinoblastoma protein. we have noted that the sequence of serine followed by a cluster of acidic amino acids is exactly the same ... | 1992 | 1425701 |
| a colposcopic case-control study of cervical squamous intraepithelial lesions in women with anogenital warts. | to assess whether anogenital warts, present or past, are an indication for women to be referred for colposcopy. | 1992 | 1427800 |
| laser use and safety. | this guidance article is an update of an article published in a special issue of health devices entitled "lasers in medicine--an introduction" (13[8], june 1984). although surgical lasers have a good overall safety record, they do expose patients, physicians, and other clinical staff to serious risks. laser hazards can cause injury, disability, or even death: hospital staff have been burned by misdirected laser beams, technicians and maintenance personnel have received eye injuries while working ... | 1992 | 1428901 |
| the natural history of carcinoma of the cervix in young women. | invasive carcinoma of the cervix was treated in 1,522 patients over a 10-year period at groote schuur hospital. data for each patient included place of residence, pathological characteristics of the tumour, nodal spread, histological evidence of human papillomavirus (hpv) infection, disease stage, treatment type, treatment complications, site of recurrence and survival time. patients aged under 35 years comprised 11.6% of the total patient group. a retrospective cohort study compared women aged ... | 1992 | 1448718 |
| recognizing the oral manifestations of aids. | the first sign of hiv infection may be an unusual or rapidly progressive condition of the oral cavity, including malignancies such as kaposi's sarcoma. early diagnosis of these oral conditions can lead to early diagnosis of hiv infection and subsequent treatment with antiretroviral agents that may improve the prognosis. this illustrated review outlines the presenting signs and symptoms of the most common oral manifestations of the aids virus, including hairy leukoplakia, candidiasis, kaposi's sa ... | 1992 | 1449978 |
| hiv disease and aids in women: current knowledge and a research agenda. | the study of the clinical manifestations, progression, and outcome of human immunodeficiency virus (hiv) infection in women has begun in earnest. aids-defining diseases that are more common in women than in men include wasting syndrome, esophageal candidiasis, and herpes simplex virus disease, whereas kaposi's sarcoma is rare. non-aids-defining gynecological conditions such as vaginal candida infections and cervical pathology are prevalent among women at all stages of hiv infection. associations ... | 1992 | 1453325 |
| epidemiology of adult sexually transmitted disease agents in children being evaluated for sexual abuse. | this prospective study describes the epidemiology of adult sexually transmitted disease agents in 1538 children ages 1 to 12 years being evaluated for possible sexual abuse. infections with these agents were related to the presence or absence of a history of sexual contact. neisseria gonorrhoeae (gc) was found in 2.8% (41 of 1469); human papillomavirus presenting as condyloma acuminata, 1.8%; chlamydia trachomatis, 1.2% (17 of 1473); treponema pallidum (syphilis), 0.1% (1 of 1263); and herpes si ... | 1992 | 1454437 |
| human immunodeficiency virus as a risk factor in miscellaneous cancers. | the association of malignancies, such as non-hodgkin's lymphoma and kaposi's sarcoma, with human immunodeficiency virus infection has been recognized since the beginning of the epidemic. however, an increasing number of tumors not diagnostic of acquired immunodeficiency syndrome has been described in this setting. taking into consideration that survival of patients with human immunodeficiency virus infection is increasing because of improvement of supportive care and better control of human immu ... | 1992 | 1457506 |
| the transcriptional transactivation function of wild-type p53 is inhibited by sv40 large t-antigen and by hpv-16 e6 oncoprotein. | the observed interaction between p53 and the oncoproteins encoded by several dna tumor viruses suggests that these viruses mediate their transforming activities at least in part by altering the normal growth regulatory function of p53. in this study we examined the effect of viral oncoprotein expression on the transcriptional transactivation function of wild-type p53 in human cells. plasmids expressing human p53 were cotransfected with either sv40 large t-antigen or human papillomavirus (hpv) ty ... | 1992 | 1464323 |
| [lichen ruber hypertrophicus with verrucous carcinoma]. | a 51-year-old patient presented with aggregated papules and with a central, partly erosive plaque on the left lower leg. histological examination revealed hypertrophic lichen planus peripherally and a verrucous carcinoma within the centre. the lesion was removed surgically. shortly after wards an atrophic lichen planus developed exclusively within the skin graft, which was effectively treated with topical steroid cream. the patient remained disease-free for 2 years. the literature relating to ma ... | 1992 | 1468932 |
| [four years of screening cervical smears of cervical lesions]. | twelve thousand two hundred and eighty nine pap smears were collected from public hospitals and from private practices during a four year period (january 1987 to december 1990). 4.2% of pap smears exhibited condylomatous or dysplastic lesions. 94.5% of such lesions were encountered in pap smears taken from the transformation zone and which contained endocervical cells. therefore, these smears represent the only adequate sample for cervical cancer screening. in our study, a close concertation bet ... | 1992 | 1469229 |
| absence of genomes of dna tumor viruses and expression of oncogenes and growth factors in two esophageal carcinoma cell lines of chinese origin. | to study the oncogenesis of human esophageal carcinoma, the presence of dna sequences homologous to several dna tumor viruses and the expression of oncogenes and growth factor genes were examined in two esophageal carcinoma cell lines of chinese origin, ce48t/vgh and ce81t/vgh. southern blot analyses failed to detect sequences homologous to hepatitis b virus (hbv), epstein-barr virus (ebv), herpes simplex virus type 2 (hsv-2), cytomegalovirus (cmv) or human papilloma virus (hpv) genomes. norther ... | 1992 | 1473373 |
| integrin expression and differentiation in transformed human epidermal cells is regulated by fibroblasts. | normal human foreskin keratinocytes (hfks) and transformed hfks (fepe1l-8 cells) generated by the introduction of cloned human papillomavirus type 16 sequences were compared for the expression and function of a family of adhesion receptors termed integrins. initially, cells were examined in conventional monolayer cultures. fepe1l-8s expressed integrins alpha 1 beta 1, alpha 2 beta 1, alpha 3 beta 1, alpha 5 beta 1, alpha 6 beta 4 and beta 1 at comparable levels to hfks. further, these receptors ... | 1992 | 1478970 |
| characterization of hpv-16 e6/e7 transcription in caski cells by quantitative pcr. | human papillomavirus (hpv) is associated with specific benign and malignant lesions of the epithelial and mucosal surfaces. of the sexually transmitted types, hpv type 16 (hpv-16) is the most commonly associated with carcinoma of the uterine cervix. expression of the e6/e7 open reading frame of the viral genome is considered critical in the development of neoplasia. using the caski cervical carcinoma cell line as a model system, we have adapted the polymerase chain reaction to quantify the trans ... | 1992 | 1480186 |
| status of the p53 tumor suppressor gene in human squamous carcinoma cell lines. | dominant-negative and/or loss-of-function mutations of the p53 tumor suppressor gene are frequently found in squamous cell carcinomas of the skin and of the head-and-neck region. in order to identify the precise mechanisms of inactivation of p53 in tumors of this class, we examined the status of p53 rna, protein and dna in a panel of eight human squamous carcinoma cell lines (head-and-neck, 3; esophagus, 1; lung, 1; uterine cervix, 2; vulva, 1). three lines (a253, calu-1, sqcc/y1) failed to expr ... | 1992 | 1486218 |
| laryngeal papillomatosis. | the papilloma is one of the most common benign neoplasms of the larynx. laryngeal papillomatosis is a disease of widespread papilloma formation that most commonly affects the larynx but may involve multiple areas of the aerodigestive tract. typically, onset of disease during childhood is associated with much more aggressive disease. the etiology of this disease is the human papillomavirus. diagnosis is often delayed because the symptoms mimic a variety of other diseases. laryngeal papillomatosis ... | 1992 | 1494048 |