| human papillomavirus (hpv) types specific of epidermodysplasia verruciformis detected in warts induced by hpv3 or hpv3-related types in immunosuppressed patients. | epidermodysplasia verruciformis (ev) is characterized by an abnormal genetic predisposition to infection with specific types of human papillomavirus (hpv). specific defects of the cell-mediated immunity and/or of the control of hpv infection in keratinocytes are assumed to be involved in the development of the disease. as a model to test this hypothesis, we have studied the prevalence of ev-specific hpv in skin warts of 56 immunosuppressed patients. all main types of cutaneous hpv (hpv1, 2, 3, 4 ... | 1992 | 1317396 |
| human papillomavirus dna in cervix. in-situ hybridization with biotinylated probes on bouin's fixed paraffin embedded specimens. | we examined retrospectively a series of 65 bouin's fixed, paraffin-embedded tissue specimens from 8 condylomatous lesions, 16 condylomas associated with cervical intraepithelial neoplasia (cin), and 12 neoplasia without condylomatous signs, for histological characteristics, the detection of viral structural antigen, the presence and typing of hpv dna by molecular in situ hybridization with biotinylated probes types 6, 11, 16 and 18 under stringent conditions (tm - 12 degrees c). hpv dna was pres ... | 1992 | 1317560 |
| 'hit and run' oncogenesis by human papillomavirus type 18 dna. | transfection of an immortalized cell line (ae), derived from syrian hamster embryo cells, with human papillomavirus type 18 (hpv 18) dna induced morphological transformation and these transformed cells were tumorigenic in nude mice. southern blot analysis revealed that the transfected viral dna was retained in all the cell lines tested, however, all these transformed cells contained only less than one copy per cell of viral genome. eleven cloned cell lines were established from a tumor cell line ... | 1992 | 1317646 |
| integration of human papillomavirus types 16 and 18 in cervical adenocarcinoma. | to determine which type of human papillomavirus (hpv) is associated with cervical adenocarcinoma and whether the virus was integrated or episomal in two continents. | 1992 | 1317883 |
| detection of high risk human papillomavirus in routine cervical smears: strategy for screening. | to develop a methodology for direct detection of high risk human papillomavirus (hpv) infection in routine cervical smears by non-isotopic in situ hybridisation (nish) which can be compared with cytopathological assessment of the same cells. | 1992 | 1317884 |
| basal cell carcinoma of the scrotum. report of three cases and review of the literature. | although basal cell carcinoma (bcc) is the most common human malignancy, only 21 cases involving the scrotum have been previously reported. | 1992 | 1317891 |
| absence of hpv 16 and 18 dna in breast cancer. | the finding that human papillomavirus (hpv) genes can immortalise breast epithelial cells has led to suggestions that hpv could be involved in the pathogenesis of breast cancer. using the polymerase chain reaction (pcr) we have been unable to demonstrate the presence of hpv dna in a series of 80 breast carcinomas. | 1992 | 1319728 |
| histological distribution of polymerase chain reaction--amplified human papillomavirus 6 and 11 dna in penile lesions. | the purpose of this study was to ascertain the histological pattern of distribution of human papillomavirus (hpv) 6 and 11 dna in penile lesions by in situ hybridization after amplification by the polymerase chain reaction (pcr). hpv dna was routinely detected by in situ hybridization with or without pcr amplification in granular layer cells that showed perinuclear halos and nuclear atypia. cells that lack these histological features rarely exhibited hpv dna with conventional in situ hybridizati ... | 1992 | 1317997 |
| retinoic acid-mediated repression of human papillomavirus 18 transcription and different ligand regulation of the retinoic acid receptor beta gene in non-tumorigenic and tumorigenic hela hybrid cells. | human papillomavirus type 18 (hpv18) belongs to the group of genital papillomaviruses involved in the development of cervical carcinomas. since retinoic acid (ra) is a key regulator of epithelial cell differentiation and a growth inhibitor in vitro of hpv18-positive hela cervical carcinoma cells, we have used hela and hela hybrid cells in order to analyse the effects of ra on expression of the hpv18 e6 and e7 oncogenes and of the cellular ra receptor genes rar-beta and -gamma. we show here that ... | 1992 | 1318198 |
| prevalence of human papillomavirus (hpv) infection in basque country women using slot-blot hybridization: a survey of women at low risk of developing cervical cancer. | cervical smears from 1,178 women with cytologically normal cervices and 67 women with cervical intra-epithelial neoplasia (cin) or cervical carcinoma were analyzed for the presence of hpv 6, 11, 16 and 18 dna by slot-blot hybridization. hpv dna was detected in 17% (95% ci: 14%-19%) of the women with a normal smear; 11% of infected women harboured hpv 16 and 18 (95% ci: 9%-13%, each). hpv dna was detected in 54% (95% ci: 41%-66%) of patients with abnormal smear; the most prevalent virus type in t ... | 1992 | 1318269 |
| [human papillomavirus (hpv) dna infections of the uterine cervix]. | 411 women who had dysplasia, selected from an ambulatory group as well as 240 women from a random control group were examined, by using cervical smears, which were initially diagnosed as human papilloma viruses-dna (hpv-dna) of the type 6/11, or 16/18, or 31/33/35. this was achieved by the in-situ nucleic acid hybridisation technique. the results of the hpv-dna typing were tabulated with the cytological diagnosis (munich papanicolaou (pap.) group-classification). the control group corresponding ... | 1992 | 1319373 |
| hpv 16 dna in autopsy material of a metastatic cervical carcinoma. | human papillomavirus (hpv) dna has been regularly detected in primary cervical carcinomas and in some metastatic lesions. using southern blot hybridization on autopsy material we found hpv 16 dna in a primary cervical carcinoma and in multiple metastases therefrom. | 1992 | 1318700 |
| molecular cloning of a new variant of human papillomavirus type 6 isolated from a chinese woman and expression of its l1 gene in e. coli--molecular cloning & expression of hpv6 gene. | a human papillomavirus genome dna of 7.9 kb from a chinese woman with genital condyloma acuminata was cloned in bamhi site of pat153. according to the results obtained from southern blotting, restriction mapping as well as partial dna sequencing, the isolated genome (hpv6bv) had obvious variance and was referred to as a new variant of hpv6 found in china the first time. hpv6bv l1 gene was successfully expressed in e. coli as a fusion protein with pur288. the beta-galactosidase/l1 fusion protein ... | 1992 | 1318719 |
| clinicopathologic study of arsenic-induced skin lesions: no definite association with human papillomavirus. | | 1992 | 1320060 |
| human papillomavirus-associated anogenital neoplasia in persons with hiv infection. | | 1992 | 1318737 |
| infections of the cervix uteri with human papillomavirus and chlamydia trachomatis. | apart from infection with human papillomavirus (hpv), other microorganisms may be involved in the development of cervical neoplasia. to study concomitant infections with hpv and chlamydia trachomatis, cervical specimens from 4 groups of women were examined for the presence of these microorganisms by the polymerase chain reaction. the first group consisted of 143 consecutive samples from women with no cytological abnormalities who participated in a triennial screening program to prevent cervical ... | 1992 | 1320098 |
| high prevalence of human papillomavirus transcripts in all grades of cervical intraepithelial glandular neoplasia. | cervical biopsy specimens containing cervical intraepithelial glandular neoplasia (cign) were examined for the presence of human papillomavirus (hpv) rna transcripts by in situ hybridization with iodine 125-labeled riboprobes. hpv rna was detectable in 95.2% of biopsy specimens. hpv 16 rna was present in 12, hpv 18 in 27, and both in 1 of the 42 cases examined. among hpv-positive cases, hpv rna was detectable in all grades of cign and, in three cases, in glands displaying only minimal nuclear ab ... | 1992 | 1318774 |
| co2 laser vaporization in the treatment of cervical human papillomavirus infection in women with abnormal papanicolaou smears. | in a randomized study, we have evaluated the treatment of cervical human papillomavirus (hpv) lesions by co2 laser vaporization. fifty patients with abnormal papanicolaou smears and histological evidence of cervical hpv infection associated or not with cervical intraepithelial neoplasia (cin) grade i were randomized to either a treatment or a control group. the cervical swabs were obtained every 3 months in both groups and examined for hpv type 16 dna by the polymerase chain reaction. after a fo ... | 1992 | 1319385 |
| vulvar manifestations of human papillomavirus infection. | as technology continues to improve, we are increasingly able to detect the presence of hpv in both clinically diseased and histologically and clinically normal vulvar tissue. unfortunately, the technology for effective treatment of these conditions has not advanced as rapidly. still, fundamental information about the interaction of hpv with the host cell continues to be collected and will provide us with an increased understanding of the importance of latent infection and its progression to clin ... | 1992 | 1318813 |
| prevalence of epstein-barr virus and human papillomavirus in oral mucosa of hiv-infected patients. | epstein-barr virus (ebv) has been implicated in the genesis of oral hairy leukoplakia (ohl). initially, ohl was also associated with human papillomavirus (hpv) as evidenced by staining with antiserum to papillomavirus common structural antigens and reports of two hpv-positive ohl as detected by in situ dna hybridization. the aims of this study were to determine the prevalence of ebv and hpv dna in ohl and normal oral mucosa and to explain the basis for the staining of ohl tissues with antibodies ... | 1992 | 1318379 |
| human papillomavirus detection from the female genital tract: combined vs. separate scrape methods. | because genital human papillomavirus (hpv) infections tend to be multifocal, it was studied how effective one combined specimen is in detecting hpv-dna from the lower female genital tract. the study population consisted of 50 patients referred to a colposcopy clinic for a suspected condylomatous and/or dysplastic lesion. from half of the patients, a separate scrape from the cervix, vagina and vulva was taken first followed by a combined scrape representing all the genital sites, and from the oth ... | 1992 | 1318854 |
| early promoters of genital and cutaneous human papillomaviruses are differentially regulated by the bovine papillomavirus type 1 e2 gene product. | the physical state of the human papillomavirus (hpv) genome is usually different in malignant lesions of the skin, in which it is generally found in episomal form, and genital mucosa, in which it is frequently integrated with disruption of the e2 gene. using chimeric or natural hpv promoters in the presence of the bovine papillomavirus type 1 e2 gene product, we observed transcription activation or repression, depending on the distance of e2-binding motifs from the start site. we found a clear d ... | 1992 | 1318941 |
| [buschke-loewenstein's tumor]. | buschke-loewenstein tumour, also called giant condyloma acuminatum, is located on the external genitalia and in the perineal region. it presents as a craggy, burgeoning and locally invasive mass that looks clinically worrying. histology shows a classically benign papillomatous proliferation, but transformation into invasive carcinoma occurs in about one-third of the cases. local penetration and sometimes focal cytological atypias have prompted certain authors to use the term verrucous carcinoma. ... | 1992 | 1319054 |
| mutant p53 can substitute for human papillomavirus type 16 e6 in immortalization of human keratinocytes but does not have e6-associated trans-activation or transforming activity. | human papillomavirus type 16 (hpv16) e6 and e7 are selectively retained and expressed in hpv16-associated human genital tumors. e6 is active in several cell culture assays, including transformation of nih 3t3 cells, trans activation of the adenovirus e2 promoter, and cooperation with e7 to immortalize normal human keratinocytes. biochemically, the hpv16 e6 protein has been shown to bind to tumor suppressor protein p53 in vitro and induce its degradation in a rabbit reticulocyte lysate. to examin ... | 1992 | 1318401 |
| inhibition of tumorigenicity of cervical cancer cells in nude mice by hpv e6-e7 anti-sense rna. | | 1992 | 1319412 |
| etiology of breast carcinoma: no apparent role for papillomavirus types 6/11/16/18. | a recent study has shown that human papillomavirus (hpv) types 16 and 18 can immortalize normal breast epithelium, and raised the possibility that hpv may be etiologically related to some cases of breast cancer. in order to investigate this possibility, we performed polymerase chain reaction (pcr) assays for hpv types 6, 11, 16 and 18 in 15 papillomas, 15 papillary carcinomas, and 13 infiltrating ductal carcinomas of the breast. no hpv-related dna sequences were identified by southern blotting o ... | 1992 | 1320761 |
| detection of multiple types of human papillomavirus in a giant condyloma from a grafted patient. analysis by immunohistochemistry, in situ hybridisation, southern blot and polymerase chain reaction. | immunosuppressed patients such as transplant recipients are known to develop multiple lesions suggestive of human papillomavirus (hpv) infection. a giant anal condyloma was obtained from a transplant patient; several fragments taken from different areas were examined for the presence of hpv dna using in situ hybridisation, polymerase chain reaction (pcr) and southern blot. typical koilocytes were seen in routinely stained tissue sections, suggesting an hpv infection; furthermore, group specific ... | 1992 | 1320793 |
| seroreactivity to hpv-16 proteins in women with early cervical neoplasia. | although serological reactivity to human papillomavirus type 16 (hpv-16) proteins has been demonstrated in patients with invasive cervical carcinoma, the degree of seroreactivity to these proteins in women with preinvasive disease and its relationship to the hpv type associated with the disease are unclear. we obtained sera from 27 women undergoing cone biopsy for cervical precursor lesions and 22 controls and analyzed seroreactivity by western blot to fusion proteins containing portions of the ... | 1992 | 1319282 |
| human papillomavirus type 11 in multiple squamous cell carcinomas in a patient with subacute cutaneous lupus erythematosus. | squamous cell carcinoma (scc) is a rare complication of cutaneous lupus erythematosus (le). aside from the documented role of ultraviolet light, scc may arise in areas of chronic scarring, such as in lesions of discoid le. iatrogenic immunosuppression associated with organ transplantation also results in a predisposition to scc, often with multiple tumors. a role for certain human papillomaviruses (hpv) in the development of squamous cell carcinoma has been documented; specifically, hpv types 5 ... | 1992 | 1319432 |
| human papillomavirus and herpes virus dna are not detected in benign and malignant prostatic tissue using the polymerase chain reaction. | fresh prostatic tissue removed at the time of surgery was assayed for the presence of human papillomavirus (hpv) types 6, 11, 16, 18 and 33 and herpes and varicella-zoster viruses (hv) using dna amplification followed by specific hybridization. thirty samples representing both benign and malignant prostatic disease were assayed. although appropriate amplimers were present for beta globulin gene indicating successful extraction of dna, no hpv or hv amplimers could be obtained with appropriate pri ... | 1992 | 1319507 |
| [virus and cancer]. | there are at least four viruses tightly associated with human cancer: htly-i and htly-ii with certain leukemias, ebv with lymphomas, bhv with hepatocarcinomas and hpv with genital cancer. in this work we discuss some evidences indicating these associations; in particular we emphasize the characteristics of human papillomavirus (hpv), due to its growing importance in the development of uterine-cervix carcinoma and the mortality of mexican women. the low percentage of infected individuals that dev ... | 1992 | 1319613 |
| detection of human papillomavirus type 16 dna sequences in archival cervical tissues by the polymerase chain reaction. | we have evaluated the polymerase chain reaction for the detection of viral dna sequences in paraffin-embedded archival tissues. in 63 frozen cervical biopsy specimens that were taken from premalignant and invasive lesions, southern blotting detected human papillomavirus (hpv) type 16 dna in 28 (44%) of the samples. in the polymerase chain reaction analysis of the formalin-fixed, paraffin-embedded mirror biopsy specimens, 46 (73%) of the tissues were found to be positive for hpv type 16. in three ... | 1992 | 1319702 |
| screening for high- and low-risk human papillomavirus types in single routine cervical smears by non-isotopic in situ hybridization. | routine cervical smears (n = 262) from a sexually transmitted diseases clinic were screened by non-isotopic in situ hybridization (nish) stratifying human papillomavirus (hpv) infections into hpv6/11 (low risk) and hpv16/18/33 (high risk) categories. of 188 patients with cytologically normal smears, hpv sequences were demonstrated in 41%. of the 128 cases analysed by dual nish, 16% contained low risk, 20% high risk and 5% both groups. in patients with cytological evidence of wart virus infection ... | 1992 | 1319766 |
| human papillomavirus type 16 (hpv 16) gene expression and dna replication in cervical neoplasia: analysis by in situ hybridization. | we have analyzed human papillomavirus (hpv) type 16 rna expression in premalignant cervical lesions of different severity and in squamous cervical cancers by rna-rna in situ hybridization in order to find differences in the topographic distribution of viral rna, which might correlate with the severity of disease. in the basal layer of low-grade squamous intraepithelial lesions (sil) only weak transcription of viral early genes was observed. signal intensity increased strongly in the more differe ... | 1992 | 1318602 |
| human papillomavirus infection and anal carcinoma. retrospective analysis by in situ hybridization and the polymerase chain reaction. | to examine the association of human papillomavirus (hpv) infection with anal squamous cell carcinoma, the authors applied the highly sensitive polymerase chain reaction (pcr) and in situ hybridization (ish) techniques to detect hpv dna in formalin-fixed, paraffin-embedded tissues from 18 patients. the presence of hpv types 16/18 in 3 (16.7%) of 18 patients with anal carcinoma was found, using a colorimetric ish technique for hpv types 6, 11, 16, 18, 31, 35, and 51. results from one of these thre ... | 1992 | 1318640 |
| transcriptional enhancer factor (tef)-1 and its cell-specific co-activator activate human papillomavirus-16 e6 and e7 oncogene transcription in keratinocytes and cervical carcinoma cells. | the human papillomavirus (hpv)-16 oncogenes, e6 and e7, are transcribed preferentially in keratinocytes and cervical carcinoma cells due to a 5' enhancer. an abundant peptide binding to a 37 nt enhancer element was purified from human keratinocytes by sequence-specific dna chromatography. this protein was identified as transcriptional enhancer factor (tef)-1 by complex mobility, binding to wild-type and mutant sv40 and hpv-16 enhansons and antigenic reactivity with two anti-tef-1 antibodies. tef ... | 1992 | 1318197 |
| histological human papillomavirus induced lesions: typization by molecular hybridization techniques. | the viral typization in human papillomavirus (hpv) infections of the lower female genital tract is relevant both from the epidemiological and the clinical point of view. we have tested dna from specimens obtained by guided biopsies on neoplastic and benign lesions, using single probes of the different virus types (6, 11, 16 and 18) by dot-blot and southern blot analysis. according to previous studies hpv 16 and 18 have been detected in neoplastic lesions, while 6 and 11 were more frequently foun ... | 1992 | 1319902 |
| human papillomavirus infection of the female genital tract: correlation of hpv dna with cytologic, colposcopic, and natural history findings. | although molecular biology evidence suggests a strong link between hpvs and anogenital neoplasias, evidence from clinical studies is still less convincing. thus, the recognition of both overt and subclinical hpv infection has become increasingly important. we studied the correlation between hpv dna and cytologic, colposcopic and natural history findings in a defined group of women. the study population consisted of 150 hpv dna positive cases (mean age 25.7 years, sd 7.4) and 69 randomly selected ... | 1992 | 1319903 |
| nucleolar organizing regions in human papillomavirus infection and in cervical intraepithelial neoplasia. | the ag-nor staining technique for nucleolar organizer regions (ag-nors) was applied to 37 biopsies of the uterine cervix taken from colposcopically abnormal areas. histological examination of the sections showed that 13 patients had condylomatous lesions without atypia, 15 had cin i, 4 had cin ii, 3 had cin iii and 2 carcinoma in situ. the mean numbers of ag-nors in parabasal and basal cells of squamous epithelium increased progressively from cin i to cin iii-cis. there was no significant differ ... | 1992 | 1319904 |
| prevalence of human papillomavirus infections among heterosexual men and women with multiple sexual partners. | a prospective study of 65 men and 111 women with multiple heterosexual partners was designed to assess the prevalence and potential risk factors of genital human papillomavirus (hpv) infections. in addition, the hpv reservoir in genital, rectal, and oral mucosa was examined. the specimens for the detection of hpv dna were taken from different sites such as the urethra and coronal sulcus (men), cervix and labia minora (women), anus, rectum, tongue, and buccal mucosa (both men and women). women un ... | 1992 | 1320094 |
| comparison of cervical cytology and the polymerase chain reaction for hpv 16 to identify women with cervical disease in a general practice population. | a comparison of the ability of cervical cytology and the polymerase chain reaction (pcr) for human papilloma virus type 16 (hpv 16) to identify women with cervical disease has been performed in a general practice population of 249 women, none of whom were believed to have current cervical disease prior to examination. within this population, 29 women were found by colposcopy and subsequent histopathology to have evidence of cervical disease [5 with cervical intraepithelial neoplasia (cin) 3; 8 w ... | 1992 | 1320102 |
| abnormal papanicolaou smears. comparison of cytology, colposcopy and cervical swab dna hybridization. | cervical swab sampling for dna hybridization was performed on 89 women attending a colposcopy clinic. in situ hybridization studies for human papillomavirus (hpv) types 6/11, 16/18 and 31/33/35 were performed on the specimens. biopsy specimens were obtained from 65 patients. the cytologic, colposcopic and histologic results were classified into five groups and assigned a score of 0-5, depending upon the diagnosis. an increasing score correlated positively with the presence of hpv, especially typ ... | 1992 | 1320120 |
| construction of an expression vector for human papillomavirus type 16 e7-glutathione s transferase fusion protein. | an expression vector for human papillomavirus type 16 (hpv 16) e7-glutathione s transferase fusion protein was constructed. the e7 gene was confirmed by southern blot analysis with a digoxygenin 11-dutp labeled and simultaneously amplified e7 dna probe by polymerase chain reaction (pcr). the fusion protein was expressed in e. coli, recovered as inclusion bodies, and was confirmed by western blot analysis using a monoclonal antibody against hpv 16-e7 protein. a polyclonal antibody against the hpv ... | 1992 | 1320148 |
| genital human papillomavirus infection. | | 1992 | 1320240 |
| human papillomavirus associated with bladder cancer. | recently published data have suggested a link between active human papillomavirus (hpv) infection and the development of bladder cancer. this study was undertaken to test for hpv genomic material in the tumors of patients without evidence of ongoing viral infection. twenty-three consecutive patients with clinical evidence of intravesical neoplasia and no history of hpv infection or clinical evidence of intercurrent disease, underwent cystopanendoscopy and biopsy as part of the routine evaluation ... | 1992 | 1320302 |
| detection of human papillomavirus in primary hepatocellular carcinoma. | human papillomaviruses (hpv) have been linked causally to some human cancers such as cervical carcinoma. to determine whether any additional type of human malignancy contained hpv dna, we examined 16 hepatocellular carcinoma (hcc) specimens by southern blot technique and by polymerase chain reaction (pcr). one hcc contained hpv 16 dna as demonstrated by both southern blot and pcr. two other hcc samples contained hpv 18-related nucleotide sequences by pcr but were negative by southern blot of gen ... | 1992 | 1320356 |
| predominance of serum antibodies to synthetic peptide stemming from hpv 18 open reading frame e2 in cervical adenocarcinoma. | to determine if there are type specific differences in serum antibody responses to synthetic peptides derived from human papillomavirus (hpv) open reading frame (orf) e2 in patients with cervical carcinoma. | 1992 | 1320634 |
| papilloma virus in cervical carcinoma: detection of viral antigen in cancer cells. | paraffin sections of biopsies from histopathologically confirmed cases of uterine cervical carcinoma, cervical scrapings from dysplasia, chronic cervicitis, tumour cells from carcinoma of the oral cavity and normal tissues from healthy normal cervix and oral cavity scrapings were examined for the presence of human papilloma virus antigens. the techniques adopted were the indirect immunofluorescence stainig and the peroxide-anti-peroxidase techniques. the hpv-antigen was present in 38 percent and ... | 1992 | 1320667 |
| characterization of primary human fibroblasts transformed by human papilloma virus type 16 and herpes simplex virus type 2 dna sequences. | human papilloma virus type 16 (hpv-16) and herpes simplex virus type 2 (hsv-2) are human viruses implicated in the development of cancer, in particular cervical cancer. the ability of hsv-2 and hpv-16 to transform early passage human cells was examined in this report. for these studies, gingival fibroblasts were utilized. one gingival cell strain was derived from a normal individual (n-16). the second cell strain was derived from hyperplastic gingival tissue of an epileptic individual (r-30) tre ... | 1992 | 1321874 |
| detection of herpes simplex virus type-2 dna and human papilloma virus dna sequences in cervical carcinoma tissue by molecular hybridization. | biopsy samples from one hundred and two patients with squamous cell carcinoma of the uterine cervix and tissues from twelve healthy normal cervical tissues (post hysterectomy) were examined for hsv-type 2 and hpv-11 dna sequences by molecular hybridization technique. in the carcinoma tissue extracts 53% contained hsv-2 dna, 27% -hsv-1-dna and 36% showed hpv-11 gene sequences while 5.7% were found to contain both hpv and hsv-2 dna. biopsies from healthy cervix were completely negative for hsv-2 a ... | 1992 | 1320668 |
| human papillomavirus type 16 in a homosexual man. association with perianal carcinoma in situ and condyloma acuminatum. | the association of anal carcinoma with human papillomavirus (hpv) type 16 infection is well documented. anal carcinoma is also frequently associated with a history of anogenital condylomata. more than 90% of anogenital condylomata contain hpv type 6 or 11. it is rare for a condylomatous lesion to contain hpv 16. we report the unusual case of a homosexual man, testing positively for human immunodeficiency virus, with carcinoma in situ evolving within perianal condylomata infected with hpv 16. | 1992 | 1320850 |
| patterns and implications of subclinical vulval human papillomavirus infection: the impact of pcr analysis. | this study was performed to elucidate the presence of human papillomavirus (hpv) infection of the vulva by colposcopy, histology and the polymerase chain reaction (pcr). colposcopy defined 5 patterns of vulval epithelial lesions inconspicuous to the naked eye. of these 75 subclinical vulval lesions, hpv infection was diagnosed by histology in 20.0% of minor epithelial changes with faint acetowhitening, 52.2% of conspicuous acetowhite lesions, 63.0% of acetowhite areas with satellite lesions, 84. ... | 1992 | 1320855 |
| pcr for the detection of genital human papillomavirus infection: a mixed blessing. | | 1992 | 1320898 |
| human papillomavirus in breast cancer. | histological sections from paraffin-embedded breast carcinoma and axillary lymph nodes were examined for the presence of human papillomaviruses by two different techniques: the polymerase chain reaction (pcr) and the in situ hybridization with biotin-labelled probes. by pcr we detected hpv 16 dna sequences in 29.4% of breast tumours and in some metastatic lymph nodes, though we were unable to identify any hpv dna sequences by in situ hybridization. these results suggest that hpv's could play a r ... | 1992 | 1320958 |
| impact of human papillomavirus research on the histopathologic concepts of genital neoplasms. | | 1992 | 1321025 |
| targeted degradation of the retinoblastoma protein by human papillomavirus e7-e6 fusion proteins. | the e6 and the e7 proteins of the oncogenic human papillomavirus types 16 and 18 can stably associate with p53 and the retinoblastoma protein, respectively. the e6-p53 interaction results in the accelerated degradation of p53 in vitro via the ubiquitin-dependent proteolysis system. in this study we demonstrate that a fusion protein consisting of the n-terminal half of the hpv-16 e7 protein and the full length hpv-16 e6 protein promotes the in vitro degradation of the retinoblastoma protein. this ... | 1992 | 1321031 |
| [role of langerhans' cells against cervical human papilloma virus infection and development of cervical carcinoma]. | human papilloma virus (hpv) in the development of cervicitis and cervical carcinoma, and the role of langerhans' cells (lc) against cervical hpv infection and development of cervical carcinoma were studied by immunohistochemistry and by dna molecular hybridization techniques for the hpv type 6, 11, 16 and 18 dna sequences. the total results were analysed and correlated. it was found that the positive rate of hpv type 6 and 11 in cervicitis was significantly higher than that in cervical carcinoma ... | 1992 | 1319807 |
| general primer polymerase chain reaction in combination with sequence analysis for identification of potentially novel human papillomavirus genotypes in cervical lesions. | we recently described the detection of potentially novel human papillomaviruses (hpv) genotypes (hpv types x [hpv x]) in cervical smears (a. j. c. van den brule, c. j. l. m. meijer, v. bakels, p. kenemans, and j. m. m. walboomers, j. clin. microbiol. 28:2739-2743, 1990) by using the general primer-mediated polymerase chain reaction method (gp-pcr). in this study, the hpv specificities of gp-pcr products were determined by sequence analyses. m13 bacteriophage clones of pcr products derived from c ... | 1992 | 1321168 |
| detection of human papillomavirus dna sequences in cervical lesions by in situ hybridization using biotinylated dna probes. | ninety-six biopsy specimens were collected for studying the association of specific sequences of hpv dna with genital warts, precancerous lesions and invasive cervical cancer. using dna probes labeled with biotin, 17 of the 22 (77.27%) specimens showing condyloma and 1 of the 14 (7.14%) specimens displaying subclinical papillomavirus infection (spi) of cervix were found to be positive for hpv 6/11; 4 of 23 (17.39%) specimens of squamous epithelial cervical cancer and 2 of the 23 (8.70%) specimen ... | 1992 | 1319885 |
| detection of the human papilloma virus type 16 mrna-transcripts in cytological abnormal scrapings. | the rna-polymerase chain reaction (pcr) was carried out on cervical scrapings to detect and analyze transcripts from the e6-e7 open reading frames (orf) of human papilloma virus type 16 (hpv16). the method, described previously for cervical squamous carcinomas and cervical intraepithelial neoplasias, was adapted to cervical scrapings. a primer set and two different probes specific for the e6-e7 orfs were selected. one of the probes was able to detect the amplification products from the full leng ... | 1992 | 1321226 |
| conservation of hpv-16 e6/e7 orf sequences in a cervical carcinoma. | a cervical carcinoma that contained human papillomavirus (hpv)-16 homologous dna was analyzed. each tumor cell genome contained a single, incomplete copy of hpv-16 dna. the e6 and e7 open reading frames (orfs) were completely conserved relative to other published hpv-16 sequences. much of the non-coding region (ncr) was free of base changes, including complete conservation of several regulatory elements. multiple mutations were identified in the remaining integrated hpv-16 dna, which was compose ... | 1992 | 1321227 |
| metal thiolate coordination in the e7 proteins of human papilloma virus 16 and cottontail rabbit papilloma virus as expressed in escherichia coli. | the oncogenic e7 proteins of human papilloma virus (hpv 16) and of cottontail rabbit papilloma virus (crpv) have been purified from an expression system in escherichia coli. the proteins as purified from e. coli contain one tightly bound zn(ii) ion per molecule. the metal site shows facile exchange with either cd(ii) or cu(i). the hpv 16 e7 maximally bound one cd(ii) or two cu(i) ions, while the crpv e7 bound two cd(ii) or three cu(i) ions. the cd(ii) and cu(i) e7 molecules exhibited optical tra ... | 1992 | 1322900 |
| transcription patterns of human papillomavirus type 16 in genital intraepithelial neoplasia: evidence for promoter usage within the e7 open reading frame during epithelial differentiation. | human papillomavirus (hpv) type 16 transcription was analysed by in situ hybridization using 125i-labelled subgenomic riboprobes, from 26 genital intraepithelial neoplastic (in) lesions, in formalin-fixed biopsies from 18 different cases. distinct transcription patterns separable by the presence or absence of late gene transcription were detected. in 12 lesions, late gene expression was absent; hpv transcripts corresponding to the e6 and e7 open reading frame (orf) were detectable in all basal c ... | 1992 | 1322961 |
| human papillomavirus (hpv) type 16 and 33 e6/e7 region transcripts in tonsillar carcinomas can originate from integrated and episomal hpv dna. | this study was undertaken to determine whether human papillomavirus (hpv) e6/e7 gene transcription in tonsillar carcinomas is correlated with viral dna integration. therefore, tonsillar carcinomas containing hpv-16 (n = 2) and hpv-33 (n = 2) dna were analysed for the viral physical state and transcription of the e6/e7 region. southern blot analysis, dna polymerase chain reaction (pcr) and, eventually, two-dimensional gel electrophoresis revealed indications for the presence of only episomal dna ... | 1992 | 1322962 |
| functional analysis of human papillomavirus type 16 e7 by complementation with adenovirus e1a mutants. | functional analysis of human papillomavirus type 16 e7 protein by complementation with adenovirus e1a mutants in baby rat kidney cells has shown that the retinoblastoma gene product (rb)-binding region of e7 can substitute in trans for that of e1a. an n-terminal e7 mutant was unable to complement an e1a mutant unable to bind p300, indicating that the two mutants were defective for functionally equivalent activities. e7 proteins with mutations within the rb-binding region were also unable to comp ... | 1992 | 1322963 |
| an evaluation of human papillomavirus testing as part of referral to colposcopy clinics. | to determine the usefulness of human papillomavirus (hpv) testing as a triage method for predicting which women referred to a colposcopy clinic were most likely to have histologically confirmed cervical intraepithelial neoplasia (cin). | 1992 | 1323087 |
| transcription of the e6 and e7 genes of human papillomavirus type 6 in anogenital condylomata is restricted to undifferentiated cell layers of the epithelium. | the e6 and e7 genes of human genital papillomaviruses (hpvs) appear to transform cells by different mechanisms. they seem to act synergistically but are not equally important when tested under diverse experimental conditions. we were therefore tempted to investigate the e6- and e7-specific transcription pattern in hpv6-infected condylomas separately, by in situ hybridization. recent studies have identified three promoters within the e6-e7 region of hpv6 and hpv11 by applying s1, exonuclease vii, ... | 1992 | 1321267 |
| identification and genetic definition of a bovine papillomavirus type 1 e7 protein and absence of a low-copy-number phenotype exhibited by e5, e6, or e7 viral mutants. | the bovine papillomavirus type 1 (bpv-1) genome replicates as a multiple-copy plasmid in murine c127 cells transformed to neoplasia by virus infection or by transfection with bpv-1 dna. it was reported previously that bpv-1 genomes harboring frameshift mutations in the e6 or e7 open reading frame (orf) replicated in c127 cells transformed by these mutants at a low copy number. furthermore, the characterization of a bpv-1 mrna in which the e6 and e7 orfs were spliced together in frame has led to ... | 1992 | 1321280 |
| interaction of the human papillomavirus type 16 e6 oncoprotein with wild-type and mutant human p53 proteins. | the e6 oncoproteins encoded by the cancer-associated human papillomaviruses (hpvs) can associate with and promote the degradation of wild-type p53 in vitro. to gain further insight into this process, the ability of hpv-16 e6 to complex with and promote the degradation of mutant forms of p53 was studied. a correlation between binding and the targeted degradation of p53 was established. mutant p53 proteins that bound hpv-16 e6 were targeted for degradation, whereas those that did not complex hpv-1 ... | 1992 | 1321290 |
| functional interaction of p53 with hpv18 e6, c-myc and h-ras in 3t3 cells. | wild-type (wt) p53 has been suggested to be the product of a tumor-suppressor gene. recently, it has been shown that the e6 oncoproteins of human papillomavirus (hpv) types 16 and 18, like the sv40 large t antigen, are physically associated with wt p53. we have investigated the functional interaction of wt p53 with the viral oncogene products of hpv16 and 18 and with cellular oncogenes by transfection of nih3t3 cells with p53 wt alone or with several oncogene(s). we found that over-expression of ... | 1992 | 1321402 |
| viral e1 and e2 proteins support replication of homologous and heterologous papillomaviral origins. | we have shown that e1 and e2 proteins of human papillomavirus type 11 (hpv-11) were essential to support the replication of the homologous viral origin (ori) in a transient replication assay, similar to reports on bovine papillomavirus type 1 (bpv-1). unexpectedly, matched or even mixed combinations of e1 and e2 proteins from hpv-11 or bpv-1 replicated either ori in human, monkey, and rodent cell lines of epithelial or fibroblastic lineage, albeit with varied efficiencies. either set of viral pr ... | 1992 | 1321423 |
| quantitation and mapping of integrated human papillomavirus on human metaphase chromosomes using a fluorescence microscope imaging system. | integrated human papillomavirus type 16 (hpv-16) dna was directly visualized on metaphase chromosomes in the two human cervical carcinoma cell lines siha and caski by fluorescence in situ hybridization with a biotinylated dna probe (7.9 kb). the fluorescence intensities of hybridization signals from single copies and dispersed clusters of integrated hpv-16 dna were quantified using a microscope equipped with a cooled-ccd camera that was interfaced to an image processor and host computer. hybridi ... | 1992 | 1321707 |
| the prevalence of hpv16dna in normal and pathological cervical scrapes using the polymerase chain reaction. | human papillomavirus (hpv) has been implicated in the development of cervical cancer. newly developed molecular techniques have facilitated hpv identification and typing of cervical lesions. several studies have suggested a strong correlation between hpv16 and intraepithelial cervical neoplasia. however, there is no agreement on this point since hpv16 is detected also in normal cervical tissue. we have amplified the l1 region of hpv16 dna using the polymerase chain reaction (pcr) technique and a ... | 1992 | 1321780 |
| association of human immunodeficiency virus and anal human papillomavirus infection among homosexual men. | a previous study of men with proctitis, proctocolitis, or enteritis showed an association of anal human papillomavirus (hpv) infection with human immunodeficiency virus (hiv) infection. because anorectal abnormalities may confound an observed association between anal hpv dna and hiv seropositivity, the present study was undertaken among consecutive homosexual men seeking hiv serologic testing who were unselected for anorectal symptoms. | 1992 | 1323247 |
| is human papillomavirus associated with cervical neoplasia in the elderly? | there have been no studies in the united states of human papillomavirus (hpv) in elderly women. this paper presents cross-sectional data on hpv and cervical neoplasia among 232 women age 65 or more. hpv deoxyribonucleic acid (dna) testing was performed using a modified dot-blot hybridization technique. the prevalence of hpv dna positivity was 3.5% (95% confidence interval (ci) 0.9%, 6.0%). there were six cases of histologic cervical neoplasia. the crude odds ratio for cervical neoplasia among hp ... | 1992 | 1321782 |
| retinoblastoma protein binding properties are dependent on 4 cysteine residues in the protein binding pocket. | the retinoblastoma gene product (prb) participates in regulating mammalian cell replication. the mechanism responsible for prb's growth regulatory activity is uncertain. however, prb is known to bind viral transforming proteins including the papilloma virus e7 protein, cellular proteins, and dna. prb contains a critical domain termed the "binding pocket" which is required for binding activities. this binding pocket contains 8 cysteine residues. a naturally occurring mutation affecting one of the ... | 1992 | 1321820 |
| [intra-lesion administration of beta-interferon in the treatment of cin associated with hpv infection]. | thirty-two women with histologically confirmed cervical intraepithelial neoplasia (cin) associated with human papillomavirus (hpv) infection were treated with intralesional beta-interferon. at 12 months from the end of the treatment, 60% of the patients showed complete regression, histologically assessed, of cin. considering separately the different cin grades, the regression for cin i was 71%, 64% for cin ii and 45% for cin iii. side-effects were rather frequent (84%) but they did not require d ... | 1992 | 1321960 |
| cytologic diagnosis of human papillomavirus. influence of age and pregnancy stage. | in a review of cytologic findings in 446,038 premenopausal women whose smears were examined during a five-year period ending in 1990, it was established that cytomorphologic evidence of human papillomavirus infection was significantly more common in the younger groups: those under 30 years of age had about twice the rate of the older groups. similarly, in a group of 29,153 pregnant women it was found that cytologic signs of human papillomavirus were reported twice as often during the second half ... | 1992 | 1321998 |
| interactions of hpv e6 and e7 oncoproteins with tumour suppressor gene products. | the hpvs associated with anogenital cancers encode two oncoproteins, e6 and e7. both e6 and e7 can form specific complexes with tumour suppressor gene products. the e7 protein binds to the retinoblastoma tumour suppressor gene product prb, with a preference for the underphosphorylated, "active" form of prb. the e7 proteins derived from the "high risk" hpvs bind to prb with a higher affinity than the e7 proteins from the "low risk" hpvs. the "high risk" hpv e6 proteins can associate with the p53 ... | 1992 | 1322242 |
| prevalence of genital papilloma virus infections in asymptomatic and symptomatic women, studied with a combined dot-blot and southern blot procedure. | a technique for the detection and typing of genital infections with human papilloma virus (hpv) is described. following a non-invasive sampling and a simplified preparation procedure, the analysis was performed as a combined dot-blot and southern blot analysis, where the former test was used to exclude cases without demonstrable content of hpv dna. the subgenomic probes used in the southern blot analyses have a higher specificity than can be achieved with genomic ones, and only one band is obtai ... | 1992 | 1323742 |
| the status of the p53 gene in human papilloma virus positive or negative cervical carcinoma cell lines. | we have analyzed p53 gene alterations in five cervical cancer derived cell lines. two of the five cervical cancer cell lines, htb31 (c-33a) and 32 (ht-3), harbored missense mutations in codons 273 and 245 respectively, whereas the other three tumor cell lines, htb33 (me180), 34 (ms751) and 35 (siha), did not reveal any mutation in the p53 coding sequence spanning codons 126-307. although all the tumor cell lines express comparable levels of p53 rna, only htb31 and htb32 contain high or detectabl ... | 1992 | 1322252 |
| prevalence and expression of human papillomavirus in tonsillar carcinomas, indicating a possible viral etiology. | the presence of human papillomavirus (hpv) dna was assessed in biopsies of tonsillar carcinomas (n = 10) and cases of tonsillitis (n = 7), serving as controls, by general-primer-mediated pcr (gp-pcr). all carcinomas appeared hpv-positive, whereas all cases of tonsillitis were hpv-negative. additional type-specific pcr for hpv 6, 11, 16, 18, 31 and 33 revealed that 4 carcinomas contained hpv 16 dna, 4 contained hpv 33 dna and 1 contained an hpv 16/33 double infection. false positivity was exclude ... | 1992 | 1322374 |
| follow-up evaluation of cervicovaginal human papillomavirus infection in adolescents. | we performed a second examination for human papillomavirus (hpv) dna on 51 sexually experienced 13- to 21-year-old (mean = 17.8 years) female patients seen at an urban teaching hospital. cervicovaginal lavages were performed 6 to 36 months apart (median = 13.3 months) to collect cells for hpv dna detection and typing by southern blot hybridization. at the first and second visits, 39.2% (20/51) and 25.5% (13/51) of patients, respectively, were infected with hpv. collectively, 56.9% (29/51) of pat ... | 1992 | 1322456 |
| human papillomavirus infections in the respiratory tract. | | 1992 | 1323936 |
| in situ hybridization at light and electron microscopic levels: identification of human papillomavirus nucleic acids. | a comparison of the sensitivities of biotinylated and 32p-labelled human papillomavirus type 6b dna probes was made. slot blot hybridization results showed that the sensitivity of biotinylated probes was consistent with that of 32p-labelled, that is, 0.1 pg of pbr 322 plasmid containing 8 kbp hpv cdna. in situ hybridization using 35s-labelled probes was applied to tissue from condylomata acuminata. after autoradiography, many silver grains were seen concentrated over the superficial koilocytic n ... | 1992 | 1322521 |
| intraepithelial neoplasia of the lower female genital tract: etiology, investigation, and management. | lower genital tract neoplasia appears associated with certain types of the human papilloma virus. surgical approaches using lasers and electricity are available to treat the clinical diseases and in turn provide good cosmetic and functional anatomy. the surgical method employed depends upon the anatomical and histological distribution of disease regardless of its site. of the three areas (cervix, vagina and vulva), cervical intraepithelial neoplasia is the least difficult to cure. vulvar intraep ... | 1992 | 1322554 |
| production of human papillomavirus and modulation of the infectious program in epithelial raft cultures. off. | human papillomaviruses trophic for anogenital epithelia cause benign warts, and certain genotypes are closely associated with cervical neoplasia. by using our modifications of the epithelial raft culture system, we were able to recapitulate and modulate the infectious program of a papillomavirus in vitro for the first time. small pieces of a condyloma containing human papillomavirus type 11 were explanted onto a dermal equivalent consisting of a collagen matrix with fibroblasts and were cultured ... | 1992 | 1321068 |
| geographical dependence of sequence variation in the e7 gene of human papillomavirus type 16. | we have determined nucleotide sequences of the e7 open reading frame (orf) of human papillomavirus type 16 (hpv-16) isolates obtained from 32 genital tumours and two hpv-16-transformed human keratinocyte cell lines. in comparison to the prototype hpv-16 isolated from a german cervical cancer biopsy, no sequence variations were noticed in either the two cell lines or the 10 biopsies that were obtained from german patients. in contrast only three of 22 (13.6%) of tanzanian isolates showed the prot ... | 1992 | 1321216 |
| sense and antisense transcripts of human papillomavirus type 16 in cervical cancers. | human papillomavirus type 16 (hpv-16) transcription was analysed in one squamous cervical carcinoma by cdna cloning and dna sequencing, and in eight additional squamous cervical carcinomas and 11 precancerous lesions by rna-rna in situ hybridization. the nucleotide sequences of the cdna clones revealed structures of early hpv-16 mrnas (e6*-e7-e1 e4-e5) in agreement with data reported for other premalignant and malignant tumours. cdna clones possibly representing viral rna of antisense orientatio ... | 1992 | 1321217 |
| human papillomavirus-associated lesions of the vagina and cervix. treatment with a laser and topical 5-fluorouracil. | twenty women with cervical and vaginal human papillomavirus-associated lesions were treated with co2 laser ablation followed by eight weekly applications of 5-fluorouracil. viral subtyping in a majority of patients and histology were obtained before and after treatment. after treatment 88% (15 of 17) had normal vaginal biopsies, and 59% (10 of 17) had normal cervical biopsies. there were no treatment failures with subtype 6/11 infection of the cervix or vagina. all the failures were with viral s ... | 1992 | 1324311 |
| human papillomavirus sequences are not detectable by southern blotting or general primer-mediated polymerase chain reaction in transitional cell tumours of the bladder. | a large series of transitional cell tumours has been screened for the presence of human papillomavirus (hpv) sequences using southern blotting and general primer-mediated polymerase chain reaction (gp-pcr). the latter technique allows the detection of a broad spectrum of both sequenced and unsequenced hpv types using two primer pairs located in the highly conserved l1 and e1 regions of the hpv genome. no evidence for hpv infection was found in 100 transitional cell tumours, 6 cases of carcinoma ... | 1992 | 1324541 |
| immortalization of fetal mouse brain glial cells by human papillomavirus type 16 e7 genes. | fetal mouse brain glial cells in primary cultures were immortalized by recombinant retroviruses containing human papillomavirus type 16 e7 genes, and named vr-2g cells. the presence and expression of e7 genes in vr-2g cells were demonstrated by the southern and the northern blot analyses. vr-2g cells did not form colonies in soft agar culture, indicating that vr-2g cells have no transforming phenotypes. by the karyotype analysis, vr-2g cells consisted of two cell populations, the pseudo-diploid ... | 1992 | 1322802 |
| [value of the in situ hybridization technique in the diagnosis of human papilloma virus infections of the uterine cervix. correlations between the human papilloma virus type and the morphological features]. | clinico-pathologic, epidemiologic and molecular analytic studies have shown that: i) the squamous cancer of the uterine cervix derives from intraepithelial lesions with different degrees of atypia; ii) human papillomavirus (hpv) can play a fundamental role in the progression of these lesions. we have examined 219 uterine cervix biopsies by means of the in situ hybridization technique (ish) using biotinylated probes for 6/11, 16/18 and 31/35/51 hpv strains. the histologic lesions were as follows: ... | 1992 | 1323097 |
| multiple hpv infection: microanatomy by in situ hybridization and immunohistochemistry. | specific human papillomavirus (hpv) types have been shown to be associated with proliferative epithelial lesions with variable biological consequences in infected patients. simultaneous infection by more than one hpv type has been infrequently reported, and its clinical significance is unknown. we have examined four biopsies of cervical and vulvar tissue, each with evidence of infection by two different hpvs. using both in situ hybridization and immunohistochemical techniques, we determined the ... | 1992 | 1323102 |
| sinonasal schneiderian papillomas: human papillomavirus typing by polymerase chain reaction. | a series of 35 formalin-fixed, paraffin-embedded schneiderian papillomas (24 inverted, nine cylindrical cell type, and two fungiform) of the nasal cavity were evaluated for the presence of human papillomavirus (hpv) types 6b/11, 16, and 18 dna sequences using both a highly sensitive and specific modification of the polymerase chain reaction technique and conventional in situ hybridization. the hpv gene sequences (e6-e7 portions) were not detected in any of the 24 inverted or nine cylindrical cel ... | 1992 | 1323107 |
| prevalence of human papillomavirus in sinonasal papillomas: a study using polymerase chain reaction and in situ hybridization. | inverted and fungiform papillomas of the sinonasal cavity share a common origin from the schneiderian membrane, but they differ widely in their rates of recurrence and progression to carcinoma. to determine the role of human papillomavirus in the etiology of these lesions, 15 inverted papillomas, five fungiform papillomas, and two squamous cell carcinomas associated with inverted papilloma were examined for the presence of hpv by in situ hybridization (ish) and polymerase chain reaction (pcr). i ... | 1992 | 1323110 |
| detection of amplified hpv 6 and 11 dna in vulvar lesions by hot start pcr in situ hybridization. | we analyzed the distribution pattern of human papillomavirus (hpv) 6 and 11 dna in vulvar lesions by in situ hybridization after amplification by the "hot start" polymerase chain reaction (pcr). hpv dna was routinely detected in granular layer cells showing perinuclear halos and nuclear atypia by in situ hybridization with or without pcr. cells that lack these changes rarely exhibited hpv dna with standard in situ hybridization. after amplification, in situ analysis showed that many of the cells ... | 1992 | 1323112 |
| correspondence re: r. a. wolber and p. b. clement, in situ dna hybridization of cervical small cell carcinoma and adenocarcinoma using biotin-labeled human papillomavirus probes. mod pathol 4:96, 1991. | | 1992 | 1323114 |
| oral condyloma lesions in patients with extensive genital human papillomavirus infection. | the incidence, location, and morphologic appearances of human papillomavirus oral lesions in patients with genital condylomatosis were investigated with clinical, colposcopic, and histologic examination as diagnostic procedures. the human papillomavirus types were also evaluated with filter in situ hybridization. | 1992 | 1323211 |
| evaluation of human papillomavirus type 5 on frozen sections of multiple lesions from transplant recipients with in situ hybridization and non-isotopic probes. | transplant recipients are at high risk to develop multiple cutaneous lesions after grafting. the frequency of the potentially oncogenic human papillomavirus (hpv) type 5 dna was evaluated in cutaneous lesions taken from sun-exposed areas in transplant recipients (92 lesions and 5 samples from normal skin) and compared with a nontransplanted population (22 lesions and 7 samples from normal skin) using in situ hybridization and biotinylated probes to hpv types 1, 2, 5, 16 and 18. hpv type 5 dna wa ... | 1992 | 1323355 |