| seroepidemiology of viral infections among intravenous drug users in northern california. | intravenous drug users are frequently exposed to parenterally transmitted viral infections, and these infections can spread to the general population through sexual activity. we investigated the prevalence of serologic markers for human immunodeficiency virus type 1 (hiv-1), human t-cell lymphotropic virus type i/ii (htlv-i/ii), hepatitis b virus (hbv), and hepatitis c virus (hcv) in intravenous drug users and their sexual contacts. of 585 drug users from northern california tested for these ser ... | 1992 | 1310362 |
| sexually transmitted viruses other than hiv and papillomavirus. | this review has focused on a select group of viruses that can be sexually transmitted. the viruses include the herpesviruses, hepatitis a virus, hepatitis b virus, delta virus, non-a, non-b hepatitis virus(es), and molluscum contagiosum. their impact on the population alone or in association with hiv disease necessitates a clear understanding of their ability to cause infection and of the manifestations of these infections. characterization of these particular pathogens and treatment have been d ... | 1992 | 1310546 |
| low frequency of p53 gene mutation in tumors induced by aflatoxin b1 in nonhuman primates. | aflatoxin b1 has been suggested as a causative agent for a g to t mutation at codon 249 in the p53 gene in human hepatocellular carcinomas from southern africa and qidong in china. to test this hypothesis, nine tumors induced by aflatoxin b1 in nonhuman primates were analyzed for mutations in the p53 gene. these included four hepatocellular carcinomas, two cholangiocarcinomas, a spindle cell carcinoma of the bile duct, a hemangioendothelial sarcoma of the liver, and an osteogenic sarcoma of the ... | 1992 | 1310637 |
| hepatitis b virus. new and evolving issues. | recent developments in molecular biology have advanced our understanding of the pathogenesis of hbv-induced disease. new data derived from the molecular analysis of clinical material have begun to bridge the gap between bench research and the clinical arena. in this review, we consider topics that have relevance to clinical management and that have not been summarized in the recent literature. the recent advances that have been made in the areas of hbv variants, in vitro cell culture systems, an ... | 1992 | 1309448 |
| hepatitis b virus markers and antibodies to hepatitis c virus in japanese patients with hepatocellular carcinoma. | sera from japanese patients with chronic liver disease were tested for hepatitis b virus (hbv) markers and antibodies to hepatitis c virus (anti-hcv), and the results were correlated to the presence of hepatocellular carcinoma. in chronic non-a, non-b liver disease, anti-hcv prevalence was high both in patients with hepatocellular carcinoma (78/89, 88%) and without it (66/84, 79%), while previous hbv infection was more common in patients with hepatocellular carcinoma (65/89, 73%) than in those w ... | 1992 | 1309450 |
| meeting report. report of meeting of section of clinical immunology and allergy, 11 march 1991. | | 1992 | 1311382 |
| incorporation of the carbocyclic analog of 2'-deoxyguanosine into the dna of herpes simplex virus and of hep-2 cells infected with herpes simplex virus. | the carbocyclic analog of 2'-deoxyguanosine (cdg) is active against herpes simplex virus (hsv), human cytomegalovirus, and human hepatitis-b virus. in order to understand the mechanism of action of this compound against hsv, we have evaluated (a) the incorporation of [3h]cdg into viral and host dna in hep-2 cells infected with hsv and (b) the interaction of the 5'-triphosphate of cdg (cdg-tp) with the hsv dna polymerase and human dna polymerases alpha, beta, and gamma (ec 2.7.7.7). incubation of ... | 1992 | 1311407 |
| p53 mutations cluster at codon 249 in hepatitis b virus-positive hepatocellular carcinomas from china. | dna samples from 36 hepatocellular carcinoma (hcc) patients from china were screened for a specific mutation affecting codon 249 of the p53 gene, recently identified as a hotspot mutation in some hccs. we detected the tumor-specific p53 codon 249 mutation in 21 (58%) of 36 hccs examined. thirteen patients with the specific codon 249 mutation had lost the remaining allele of p53, whereas the remaining eight patients appeared to have retained both copies of the gene. these results suggest that alt ... | 1992 | 1311638 |
| low risk of viral infection after administration of vapor-heated factor viii concentrate. international investigator group. | a multicenter prospective study was carried out to evaluate whether a vapor-heated factor viii concentrate transmitted blood-borne viral infections over a surveillance period of 15 months. thirty-five patients with hemophilia and von willebrand disease who had never received any blood components were treated. twenty-eight were analyzed and found not to have non-a, non-b hepatitis. sera from 20 of these 28 patients were also tested for the antibody to the hepatitis c virus. none had sero-converte ... | 1992 | 1311876 |
| aflatoxins as risk factors for hepatocellular carcinoma in humans. | on a global basis, primary liver cancer (plc) is a very prevalent form of cancer. wide variation of plc incidence in different areas of the world suggests the involvement of environmental factors in its etiology. two major classes of risk factors have been identified. extensive evidence indicates the importance of infection by the hepatitis b virus as a major risk factor for plc. because many organic chemicals induce liver cancer in experimental animals, those to which human exposure is known to ... | 1992 | 1311989 |
| antibodies to hepatitis c virus in essential mixed cryoglobulinaemia. | although essential mixed cryoglobulinaemia (emc) is recognized to be frequently associated with chronic liver disease, aetiology and pathogenesis of liver damage remain unsolved questions. the purpose of this study was to assess the possible causative role of hepatitis c virus (hcv) in the liver impairment occurring in patients with emc. twenty-six consecutive emc patients were evaluated. all patients underwent percutaneous liver biopsy. anti-hcv antibodies were assayed by elisa and supported by ... | 1992 | 1311992 |
| lack of increase in heterozygous alpha 1-antitrypsin deficiency phenotypes among patients with hepatocellular and bile duct carcinoma. | homozygous alpha 1-antitrypsin deficiency (pizz phenotype) is known to be associated with increased risk of cirrhosis and primary liver cancer. although a relationship between heterozygous alpha 1-antitrypsin deficiency and chronic liver disease was suggested recently, it is still a matter of controversy whether such patients are at increased risk of liver cancer. the goal of this study was to determine the prevalence of heterozygous alpha 1-antitrypsin deficiency of different phenotypes among p ... | 1992 | 1312055 |
| antibody to hepatitis c virus in selected groups of a canadian urban population. | in an anonymous survey, 433 sera from canadian individuals of selected categories were tested for the presence of antibody to hepatitis c virus (hcv) using a recombinant antigen-based immunoassay. about 50% of intravenous drug abusers (ivda), 10% of transfusion recipients and an overall average of 7.9% of male homosexuals were reactive for antibody to hcv. individuals with jaundice and negative hepatitis b virus (hbv) serology were not reactive for antibody to hcv compared with 26.7% of those wi ... | 1992 | 1312068 |
| cyclin a is required at two points in the human cell cycle. | cyclins play a fundamental role in regulating cell cycle events in all eukaryotic cells. the human cyclin a gene was identified as the site of integration of hepatitis b virus in a hepatocarcinoma cell line; in addition, cyclin a is associated with the e2f transcription factor in a complex which is dissociated by the e1a oncogene product. such findings suggest that cyclin a is a target for oncogenic signals. we have now found that dna synthesis and entry into mitosis are inhibited in human cells ... | 1992 | 1312467 |
| interaction of interferon-alpha with interleukin-1 beta or tumor necrosis factor-alpha on hepatitis b virus enhancer activity. | the interaction of ifn-alpha with il-1 beta or tnf-alpha on hepatitis b surface antigen (hbsag) expression was analysed in hepatitis b virus (hbv)-dna integrated plc/prf/5 and non-integrated huh-7 human hepatoma cells. secretion of hbsag in plc/prf/5 cells was reduced by ifn-alpha, il-1 beta or tnf-alpha, and synergistically depressed when ifn-alpha was used in combination with il-1 beta or tnf-alpha. by northern blot analysis, the levels of hbsag mrna were suppressed by ifn-alpha in combination ... | 1992 | 1312844 |
| a chromosome 17:7 translocation is associated with a hepatitis b virus dna integration in human hepatocellular carcinoma dna. | chronic hepatitis b virus infection is often associated with major structural rearrangements of both the integrated viral dna and the associated cellular sequences. we present here the structure of a single-copy hepatitis b virus insert cloned from human hepatocellular carcinoma dna recently reported to encode a novel transcriptional trans-activator function. the hepatitis b virus portion of the clone consists of two colinear fragments covering the x gene with its promoter and enhancer (nucleoti ... | 1992 | 1312986 |
| [acute hepatitis c. a clinical and epidemiological study]. | the discovery of the hepatitis c virus (hcv) and the possibility of determining anti-hcv serum antibodies has permitted greater etiological, clinical and epidemiological knowledge of non a non b hepatitis (nanb). | 1992 | 1313129 |
| hepatitis c virus in chronic hemodialysis patients with non-a, non-b hepatitis. | sixteen of 110 hemodialysis (hd) patients fulfilling criteria of non-a, non b hepatitis (nanbh), i.e. alanine aminotransferase (alt) greater than 50 u/ml in the absence of both serologic markers for acute hbv and hav infections and clinical evidence of another cause of hepatitis, were tested for the presence of antibodies against hepatitis c virus (anti-hcv) by enzyme immunoassay (ortho, diagnostics). all (100%) were anti-hcv-positive. there were 5 patients with a monophasic (m) rise pattern (1 ... | 1992 | 1313155 |
| regulation of hepatitis b virus gene expression by its two enhancers. | hepatitis b virus (hbv) infection causes acute and chronic hepatitis and is closely associated with the development of hepatocellular carcinoma. the principal site of hbv infection is liver, and hbv actively replicates in hepatocytes. two regions of the hbv genome have been shown previously to display properties of a transcriptional enhancer. in this study, we show that either of the two hbv enhancers can activate all three major hbv promoters in several human hepatoma lines, and the cooperative ... | 1992 | 1313564 |
| importance of primer selection for the detection of hepatitis c virus rna with the polymerase chain reaction assay. | we compared four primer sets from conserved regions of the hepatitis c virus (hcv) genome for their ability to detect hcv rna in a "nested" cdna polymerase chain reaction assay on sera from 114 anti-hcv antibody-positive individuals from around the world. the different primer sets had equivalent sensitivity, detecting less than 1 chimpanzee id50 (dose that infects 50%) when tested against reference strain h of hcv. we tested equal amounts of rna extracted from the serum of each individual with t ... | 1992 | 1309604 |
| [hepatitis-c virus and hepatoma]. | to clarify the relationship between hepatitis c virus (hcv) infection and hepatocellular carcinoma (hcc), frozen serum samples from 213 patients with histologically proven liver cirrhosis alone (96 alcoholics, 59 hbsag positive, 29 non-a, non-b hepatitis, 29 cryptogenic) and 40 patients with liver cirrhosis and hcc (12 alcoholics, 7 hbsag positive, 7 non-a, non-b hepatitis, 14 cryptogenic) were analyzed for antibodies to hepatitis c virus (anti-hcv) with the ortho-hcv-elisa. the results were as ... | 1992 | 1311125 |
| reproductive factors in the etiology of hepatocellular carcinoma. the who collaborative study of neoplasia and steroid contraceptives. | data from a hospital-based case-control study conducted in four developing countries were analyzed to evaluate the role of reproductive factors in the etiology of liver cancer. eighty-three patients newly diagnosed with primary liver cancer and 596 matched controls between the ages of 15 and 56 years completed study interviews. the relative risk of hepatocellular carcinoma was elevated significantly in women of high gravidity, an association that was attributable to the effects of full-term preg ... | 1992 | 1311212 |
| inhibition of hepatitis b virus production by modified 2',3'-dideoxy-thymidine and 2',3'-dideoxy-5-methylcytidine derivatives. in vitro and in vivo studies. | the effect of analogues of both 2',3'-dideoxy-3'-fluorothymidine (fddthd) [2',3'-dideoxy-3'-fluorouridine (fddurd), 2',3'-dideoxy-3'-fluoro-5-chlorouridine (fddclurd), 2',3'-dideoxy-3'- fluoro-5-bromouridine (fddbrurd) and 2',3'-dideoxy-3'-fluoro-5-bromovinyluridine (fddbvurd)] and 2',3'-dideoxy-3'-fluorocytidine (fddcyt) [2',3'-dideoxy-3'-fluoro-5-fluorocytidine (fddfcyt), 2',3'-dideoxy-3'-fluoro-5-chlorocytidine (fddclcyt), 2',3'-dideoxy-3'-fluoro-5-methylcytidine (fddmecyt), 2',3'-dideoxy-3'- ... | 1992 | 1314607 |
| hepatitis b virus (hbv)-specific cytotoxic t-cell response in humans: production of target cells by stable expression of hbv-encoded proteins in immortalized human b-cell lines. | to analyze the hepatitis b virus (hbv)-specific cytotoxic t-cell (ctl) response during acute and chronic viral hepatitis, target cells that express hbv-encoded antigens in the context of the appropriate hla restriction element must be available for each subject studied. since hbv is not infectious for human cells in vitro, such target cells must be produced by dna-mediated gene transfer into cultured human primary cells or cell lines. for this purpose, we have developed a panel of epstein-barr v ... | 1992 | 1313893 |
| management of persons exposed to blood. hepatitis b virus post-exposure management. | | 1992 | 1314939 |
| anti-hcv antibody in chinese cirrhotic patients with or without hepatocellular carcinoma: relation to multitransfusion. | to investigate the positive rates of antibodies to hepatitis c virus (anti-hcv) in chinese cirrhotic patients with or without hepatocellular carcinoma and to evaluate the influence of blood transfusion on the prevalence of anti-hcv in such patients, a longitudinal study in 30 cirrhotic patients (17 combined with hepatocellular carcinoma) was carried out. five patients (16.7%) were anti-hcv positive before transfusion. the positive rate of anti-hcv in hbsag-positive patients and hbsag-negative pa ... | 1992 | 1315166 |
| liver-cell dysplasia and hepatocellular carcinoma. | liver-cell dysplasia is a well known histological entity with preneoplastic significance in experimental hepatic carcinogenesis. however, while the association of liver-cell dysplasia with hepatitis b virus can be considered as established, it is still controversial whether this lesion represents a premalignant condition in cirrhotic patients. efforts have been made to render its morphological assessment more reliable, but no firm conclusions can be drawn from the available clinical studies, whi ... | 1992 | 1315175 |
| latent hepatitis b virus infection in childhood hepatocellular carcinoma. analysis by polymerase chain reaction. | the presence of hepatitis b virus (hbv) has been evaluated in liver specimens from 11 children with primary liver tumors and negative results of serologic testing for hbv markers. hbv-dna sequences were detected by the polymerase chain reaction procedure, using different sets of oligonucleotide primers from highly conserved regions of hbv genome. two of three children with histologic diagnosis of hepatocellular carcinoma were positive for hbv-dna in the liver, whereas the remaining children, inc ... | 1992 | 1315210 |
| hepatitis c virus in the etiology of chronic hepatitis and liver cirrhosis: possibility of mixed viral infections due to parenteral transmission. | sera obtained from 381 patients with chronic liver disease from four cities within the ussr were studied for hbv, hdv, and hcv markers of infection. anti-hcv activity was detected in 41.2% of non-a, non-b cases. the etiological distribution of chronic hepatitis in moscow and dushanbe was similar with an approximate 20% prevalence for hbv, hdv, and hcv infections, whereas in yakutsk 40% of cases were caused by hdv infections. the etiology of disease remained unrecognized in approximately 40% of p ... | 1992 | 1314283 |
| role of hepatitis c virus infection in spontaneous hepatitis b surface antigen clearance during chronic hepatitis b virus infection. | to examine the role of hepatitis c virus (hcv) infection in spontaneous hepatitis b surface antigen (hbsag) clearance during the course of chronic hepatitis b virus (hbv) infection, serum specimens from 32 asymptomatic hbsag carriers and 22 patients with chronic hepatitis type b who underwent spontaneous hbsag clearance were studied for antibody to hcv (anti-hcv) using commercial eias. the results were compared with those of control groups matched for age, sex, hepatitis b e antigen, antibody to ... | 1992 | 1314869 |
| [polymerase chain reaction in the detection of hepatitis b virus carriers with sole positive anti-hepatitis b core antigen]. | to assess the prevalence of serum hbv dna in the individuals with positive anti-hbc alone, seroepidemiologic investigations in three populations were carried out with polymerase chain reaction. the detection of sole anti-hbc were 21 of 294 (7.1%), 193 of 1995 (9.7%) and 23 of 377 (6.1%) individuals respectively. among the anti-hbc positive sera, hbv dna was detected in 6 (28.6%), 68 (35.2%) and 7 (30.4%) respectively. follow-up observations showed that 83.8%, 93.1% and 71.4% still remained hbv d ... | 1992 | 1315608 |
| direct gene transfer to the liver with herpes simplex virus type 1 vectors: transient production of physiologically relevant levels of circulating factor ix. | we have used gene transfer vectors derived from a replication-defective mutant of herpes simplex virus type 1 (hsv-1) expressing the hepatitis b virus surface antigen (hbsag), escherichia coli beta-galactosidase (beta-gal), or canine factor ix (cfix) from the immediate early promoter of human cytomegalovirus (hcmv) to infect mouse liver by direct injection or through the portal vein. by either route, high levels of transgene expression were demonstrated by the detection of immunoreactive hbsag o ... | 1992 | 1316145 |
| multiple oncogenes and tumor suppressor genes are structurally and functionally intact during hepatocarcinogenesis in hepatitis b virus transgenic mice. | in the current study we sought to elucidate the molecular mechanisms which might contribute to hepatocarcinogenesis in a hepatitis b virus (hbv) envelope transgenic mouse model in which chronic hepatocellular injury and inflammation lead to regenerative hyperplasia and eventually to the development of chromosomal abnormalities and hepatocellular carcinoma (hcc), thereby reiterating many of the pathophysiological events that occur prior to the development of hcc in chronic hbv infection in humans ... | 1992 | 1316229 |
| serodiagnostic assay of hepatitis c virus infection using viral proteins expressed in escherichia coli. | infection with hepatitis c virus (hcv) was analyzed by an enzyme-linked immunosorbent assay based on recombinant viral proteins encoded by regions of the putative viral core, ns3, ns4 and ns5, which were expressed in e. coli. results showed that 106 of 124 cases (85.5%) of non-a, non-b chronic hepatitis and 43 of 45 cases (95.5%) of hepatocellular carcinoma, negative for hbv marker, were positive for antibodies against at least one of these viral proteins. one of 87 healthy individuals with norm ... | 1992 | 1316340 |
| clinical evaluation of oral fluid samples for diagnosis of viral hepatitis. | oral fluid samples were compared with serum samples as a specimen source for hepatitis a, b, and c virus markers. oral fluid was obtained with a treated absorbent pad and tested by using existing commercial enzyme immunoassays with only minor modifications. compared with serum sampling the sensitivity and specificity of oral sampling were 100% (51 of 51 samples) and 98% (46 of 47 samples) for hepatitis a virus immunoglobulin m, 100% (29 of 29 samples) and 100% (29 of 29 samples) for hepatitis b ... | 1992 | 1316364 |
| sequence analysis of the hepatitis b virus pre-c region in hepatocellular carcinoma [hcc] and nontumoral liver tissues from hcc patients. | we investigated whether replication-competent pre-c/c defective mutants of hepatitis b virus (hbv) are detectable in primary human hepatocellular carcinoma (hcc) tissues from patients of a geographic area endemic for such mutants. dnas extracted from formalin-fixed paraffin-embedded hcc samples were checked for the presence of specific hbv dna sequences using the polymerase chain reaction (pcr). amplified pre-c regions from nine hcc samples were directly sequenced as were samples of nontumoral l ... | 1992 | 1316686 |
| hepatitis b virus and hepatocellular carcinoma. | | 1992 | 1317597 |
| multiple rearrangements and activated expression of c-myc induced by woodchuck hepatitis virus integration in a primary liver tumour. | woodchuck hepatitis virus (whv) is a small, partially double-stranded dna virus. like the related human hepatitis b virus (hbv), whv induces acute and chronic hepatitis and hepatocellular carcinoma (hcc) in its natural host. whv dna integration into c-myc and n-myc, resulting in deregulated expression of these genes, has been described previously in woodchuck hcc. we have analysed a woodchuck liver tumour in which whv dna was integrated in the c-myc gene. the virus insertion provoked multiple al ... | 1992 | 1317604 |
| hepatitis b virus, cigarette smoking and alcohol consumption in the development of hepatocellular carcinoma: a case-control study in fukuoka, japan. | the roles of the hepatitis b virus (hbv), cigarette smoking and alcohol consumption in the etiology of hepatocellular carcinoma (hcc) were examined in a case-control study involving 204 patients with hcc and 410 control subjects in fukuoka prefecture, where hcc risk is among the highest in japan. information on smoking and drinking habits was obtained by a detailed interview survey, and the results were analyzed in conjunction with serum hepatitis b surface antigen (hbsag) status after adjustmen ... | 1992 | 1318264 |
| alcohol consumption as a risk factor for hepatocellular carcinoma in urban southern african blacks. | our purpose was to ascertain whether alcohol abuse is a risk factor for the development of hepatocellular carcinoma in urban southern africa blacks and, if so, to relate alcohol consumption to other possible risk factors such as persistent hepatitis-b-virus infection, smoking, male sex, in this subpopulation. a prospective, hospital-based, case-control format involving 101 patients with hepatocellular carcinoma and 101 controls was used. the mean age of the patients was 53.7 +/- 1.85 years and t ... | 1992 | 1318267 |
| alternate translation initiation on hepatitis b virus x mrna produces multiple polypeptides that differentially transactivate class ii and iii promoters. | the hepatitis b virus x gene encodes a transcription activator which stimulates the synthesis of rnas from a variety of class ii and iii promoter elements. in this report, we present a mutational analysis which genetically demonstrates that the x gene actually encodes two, and possibly three, related polypeptides from a single mrna using alternate translation initiation from any of three in-frame aug codons. genetic analysis shows that translation initiates at the 5' proximal aug of x mrna and p ... | 1992 | 1318408 |
| hepatocellular carcinoma: recent progress. | | 1992 | 1314774 |
| risk assessment for aflatoxin: iii. modeling the relative risk of hepatocellular carcinoma. | estimates have been made of the cancer potency of aflatoxin exposure among the u.s. population. risk modeling is used to assess the dose-response relationship between aflatoxin exposure and primary liver cancer, controlling for hepatitis b virus (hbv), based on data provided by the yeh et al. study in china. a relative risk model is proposed as a more appropriate alternative to the additive ("absolute" risk) model for transportation of risk coefficients between populations with different baselin ... | 1992 | 1315449 |
| processing of hepatitis b virus surface antigen expressed by recombinant oka varicella vaccine virus. | we have constructed a recombinant oka varicella vaccine virus expressing hepatitis b virus (hbv) surface antigen (hbsag). hbsag was synthesized as 26k and 30k proteins in infected cells and secreted into the culture supernatant as 30k and 35k proteins. inhibitors and glycosidase treatments, and pulse-chase labelling experiments, revealed the glycosylation process of hbsag. the latter was synthesized as a non-glycosylated 26k protein and subjected to n-linked glycosylation to form a 30k protein w ... | 1992 | 1318942 |
| prevalence of antibodies to hepatitis viruses in blood donors with a clinical history of hepatitis. | a comparison between the 1979, 1982 and 1989 findings indicates that the number of adults susceptible to hav infections has increased. this fact should be given attention in view of the strongly altered travelling pattern of fairly large sections of the population. it should also be kept in mind that the group of adults born between 1951 and 1960 comprises those adults who have the most frequent contacts with younger children being at the greatest risk of acquiring hav infection. | 1992 | 1319249 |
| spontaneous regression of hepatocellular carcinoma. | we report a 65-yr-old man with hepatitis b virus-related liver cirrhosis and biopsy-proven hepatocellular carcinoma who has undergone spontaneous regression. the tumor became impalpable, and was no longer detectable by ultrasonography and computed axial tomography, 5 and 30 months later. the alpha-fetoprotein level also decreased to normal range. the clinical course is silent, and the patient is alive and well 37 months after the initial diagnosis. | 1992 | 1319672 |
| prevalence of hepatitis c virus antibody in a liver transplantation population. | the development of a serologic assay to detect antibodies directed at an antigen (c-100-3) of the hepatitis c virus (anti-hcv) has been a major breakthrough in the long search for causative agents of non-a, non-b (nanb) hepatitis. the frequency of hcv in those who have end-stage liver disease is not known. moreover, the rate of recurrence after liver transplantation (oltx) and the rate of acquisition of new hcv infection as a result of the oltx experience is as yet unknown. this study was perfor ... | 1992 | 1319888 |
| [expression and significance of pre-s1 and pre-s2 in human primary hepatic carcinoma (phc)]. | 135 specimens of primary hepatic carcinoma (phc) were formalin fixed and paraffin embedded and stained for pre-s1, pre-s2 and hbxag by abc method, for hbsag and hbcag by pap method. the detection rates of pre-s1 and pre-s2 positive cases in cancerous tissues were 22.2% and 20.0%. the detection rates of pre-s1 and pre-s2 in non-cancerous liver tissues were 60.0% and 59.6%. the positive ratio of pre-s1 and pre-s2 in the same hepatoma was 16.3% and that in the same non-cancerous liver tissue was 55 ... | 1992 | 1319893 |
| chronic liver disease in ethiopia: a clinical study with emphasis on identifying common causes. | between july 1986 and april 1989, 334 hospitalized adult ethiopian patients with chronic liver disease were studied according to a protocol to define their clinical features and to identify risk factors with the aim of preventive intervention. of these, 14 had chronic hepatitis, 208 cirrhosis and 112 hepatocellular carcinoma (hcc). both clinical and histological diagnostic criteria were employed. a detailed questionnaire was used to document demographic and clinical data. a common clinical prese ... | 1992 | 1319901 |
| glucocorticoid stimulates hepatitis b viral gene expression in cultured human hepatoma cells. | glucocorticoids have been shown to influence the severity of hepatitis b virus-related chronic hepatitis in human. however, very little is known about the effects of glucocorticoids on hepatitis b virus replication in vitro. in this report, we used a well-differentiated human hepatoma cell line, hep3b, transfected with hepatitis b virus complementary dna as a model to show that a glucocorticoid analog, dexamethasone, can directly stimulate the production of hbsag and hbeag. elevation of 3.5-kb p ... | 1992 | 1319949 |
| the mechanism of inhibition of hepatitis b virus replication by the carbocyclic analog of 2'-deoxyguanosine. | the carbocyclic analog of deoxyguanosine inhibits hepatitis b virus replication by greater than 95% in the hepatitis b virus-producing cell line (2.2.15) as monitored by decreases of secreted hepatitis b virus dna, hepatitis b virus polymerase activity and intracellular episomal hepatitis b virus dna. transcription of hepatitis b virus rna from chromosomally integrated hepatitis b virus dna was unaffected. radioactive carbocyclic 2'-deoxyguanosine was directly phosphorylated within the 2.2.15 ce ... | 1992 | 1319957 |
| hepatocellular carcinoma in porphyria cutanea tarda: frequency and factors related to its occurrence. | thirty-eight patients with porphyria cutanea tarda (pct) have been seen in the last 18 years. five of these patients (13%) developed hepatocellular carcinoma (hcc) during follow-up. we analyzed the differences in clinical, laboratory and liver histology findings at presentation, between patients who developed hcc during follow-up (hcc-group, n = 5) and those who did not (pct-group, n = 33). of the clinical features the duration of skin-symptoms was longer in the hcc-group (mean: 10.4 +/- 1.1 yea ... | 1992 | 1320175 |
| detection of human papillomavirus in primary hepatocellular carcinoma. | human papillomaviruses (hpv) have been linked causally to some human cancers such as cervical carcinoma. to determine whether any additional type of human malignancy contained hpv dna, we examined 16 hepatocellular carcinoma (hcc) specimens by southern blot technique and by polymerase chain reaction (pcr). one hcc contained hpv 16 dna as demonstrated by both southern blot and pcr. two other hcc samples contained hpv 18-related nucleotide sequences by pcr but were negative by southern blot of gen ... | 1992 | 1320356 |
| relationship between superoxide dismutase (sod) and viral liver diseases. | immunohistochemical evaluation of cu, zn- and mn-superoxide dismutase (sod) activity in various viral liver diseases was performed by the peroxidase-conjugated antibody indirect method. anti-human cu, zn-sod (rabbit) and anti-human mn-sod (guinea-pig) derived and purified from sod of human erythrocytes and placentas were used to determine sod distribution in liver tissues. sod in the liver tissues was detected in 68 inpatients of our unit. they consisted of 23 cases with chronic hepatitis caused ... | 1992 | 1320579 |
| chronic hepatitis b surface antigen-negative hepatitis after treatment of malignancy. | we reviewed the records of all patients with a diagnosis of malignancy who were treated at our center and who had not had chemotherapy for at least 18 months, to assess the prevalence of chronic hepatitis b surface antigen (hbsag)-negative hepatitis, to assess the prevalence of a marker of hepatitis c virus infection, and to determine the severity of chronic liver disease. of 557 eligible patients, 38 (6.8%) had chronic hbsag-negative hepatitis. of these 38 patients, 20 (52.6%) had a marker of h ... | 1992 | 1320673 |
| deoxycytidine deaminase-resistant stereoisomer is the active form of (+/-)-2',3'-dideoxy-3'-thiacytidine in the inhibition of hepatitis b virus replication. | 2',3'-dideoxy-3'-thiacytidine (+/-)-sddc) was found to have potent activity against human hepatitis b virus as well as human immunodeficiency viruses in culture. the (-)form ((-)-sddc) which is resistant to deoxycytidine deaminase was found to be the more active antiviral stereoisomer than the (+)-form ((+)-sddc). the (+)-sddc is susceptible to deamination by deoxycytidine deaminase and is 25- and 12-fold more toxic than (-)-sddc in cem cells in terms of anti-cell growth and anti-mitochondrial d ... | 1992 | 1321132 |
| hepatitis b vaccination: an important method of preventing hbv-related hepatocellular carcinoma. | hepatocellular carcinoma (hcc) is a multifactorial disease which requires multiple strategies for its prevention, including immunization against hepatitis type b. given the link between chronic hbv infection and hcc, vaccination against hepatitis b preventing an initial infection and the development of chronic carrier state is also likely to prevent hcc. the percentage of hcc cases attributable to hbv, which depends on both relative risk for hcc associated with the hbsag carrier state and preval ... | 1992 | 1315589 |
| [prevalence and clinical importance of antibodies to hepatitis b virus and hepatitis c virus in patients with alcoholic liver disease]. | | 1992 | 1318621 |
| [delta virus infection in hemodialyzed kidney patients and patients with chronic liver diseases]. | sera of patients with chronic hepatitis and chronic haemodialysed uraemia were tested for both hepatitis b virus and hepatitis delta virus markers from 1985. hepatitis delta virus seroconversion was found in 10 of 88 haemodialysed patients and anti-delta-igg sero-positivity in 11 of 108 chronic hepatitis patients. the clinical course of delta superinfections in haemodialysed patients was as follows: 3 infections were symptomless, 7 patients had acute hepatitis, two of the latter group died becau ... | 1992 | 1321398 |
| [hepatitis virus (hbv, hcv, hdv) markers in chronic liver diseases. comparative studies in two east-central european countries]. | the prevalence of hepatitis virus markers in patients with chronic liver diseases from two countries has been studied: 68 patients (38 alcoholic hepatitis or cirrhosis, 30 chronic hbsag-positive hepatitis) from hungary as well as 109 patients (55 alcoholic liver disease, 45 chronic hepatitis or cryptogenic cirrhosis and 9 hepatoma) from romania were examined for hbsag, anti-hbs, anti-hbc, anti-hcv and anti-hdv, using the corresponding abbott elisa test systems. in alcoholic liver disease hbsag o ... | 1992 | 1321399 |
| modification of cyclin a expression by hepatitis b virus dna integration in a hepatocellular carcinoma. | we have previously reported the identification of a hepatitis b virus (hbv) dna integration in an intron of the cyclin a gene in an early hepatocellular carcinoma (hcc) and the isolation of human cyclin a cdna. we have now constructed a cdna library from the tumor and isolated several hybrid hbv-cyclin a cdnas from it. the hybrid cdnas encode an hbv-cyclin a fusion protein. in the chimeric protein, the n-terminus of cyclin a, including the signals for cyclin degradation, is deleted and replaced ... | 1992 | 1321406 |
| does hepatitis c virus infection contribute to hepatocellular carcinoma in hong kong? | hong kong has a high incidence of hepatocellular carcinoma (hcc) and, because it is an endemic area for hepatitis b virus (hbv) infection, the etiologic association between hcc and hbv infection is reported to be as high as 80%. hepatitis c virus (hcv) recently was shown to be a possible pathogenetic agent for hcc in a number of countries. | 1992 | 1318777 |
| hepatitis c as a cause of chronic liver disease in northern pakistan. | the antibodies to hepatitis c virus (hcv) were tested in 45 histologically confirmed cases of chronic liver disease. twelve cases had chronic hepatitis, 24 cirrhosis and 9 hepatocellular carcinoma. anti-hcv was detected in 6 patients. two (16.67%) were suffering from chronic hepatitis, 3 (12.5%) had cirrhosis and one (11.11%) hepatocellular carcinoma. none of the anti-hcv positive cases had past history of blood transfusion. the patients of chronic liver disease in this study had a much higher p ... | 1992 | 1321299 |
| the prevalence of hepatitis c in a regional level i trauma center population. | several studies have examined the prevalence of hepatitis b (hbv) and human immunodeficiency virus (hiv) in a trauma population. to our knowledge, no one has reported on the prevalence of hepatitis c (hcv). we prospectively studied the prevalence of hcv, as well as hbv, hiv, and syphilis in our adult regional level i trauma center population. two hundred eighty-six consecutive trauma patients were tested for previous exposure to hcv using an anti-hcv mab elisa. patients were also tested for expo ... | 1992 | 1321915 |
| interactions of the human immunodeficiency virus and the hepatotropic viruses. | | 1992 | 1321988 |
| [occurrence of antibodies against hepatitis c virus (hcv) among drug addicts]. | the prevalence of anti-hcv and serological markers of hbv infection was tested in 100 unselected intravenous drug addicts admitted to a detoxification ward. anti-hcv were present in 78% addicts, and at least one markers of hbv infection was present in 69% addicts. no correlation was found between anti-hcv status and age, gender, duration of addiction or anti-hiv presence. furthermore, there was no difference in mean titres of anti-cmv and anti-hsv between subjects with and without anti-hcv what ... | 1992 | 1322535 |
| prevalence of hepatitis c antibodies in patients with clotting disorders in victoria. relationship with other blood borne viruses and liver disease. | to elucidate the seroepidemiology of hepatitis c in patients with clotting disorders in comparison with other blood borne infections; to examine the effects of hepatitis c on liver function; and to assess the effectiveness of current screening and inactivation procedures used in preventing the transmission of blood borne viruses by clotting factor preparations. | 1992 | 1321326 |
| human monoclonal antibodies in transplantation. | | 1992 | 1323159 |
| hepatitis b virus infection and primary hepatocellular carcinoma. | for many years, epidemiological studies have demonstrated a strong link between chronic hepatitis b virus (hbv) infection and the development of primary hepatocellular carcinoma (phc). other hepatocarcinogens such as hepatitis c virus and aflatoxin also contribute to hepatocarcinogenesis either in conjunction with hbv infection or alone. cellular and molecular biological studies are providing explanations for the hbv-phc relationship, and models are now being formulated to further test the relat ... | 1992 | 1323384 |
| polymerized albumin receptor on rat liver cells. | the polymerized albumin hypothesis was proposed for the mechanism of a hepatitis b virus (hbv) infection of human liver parenchymal cells on the basis that a receptor for polymerized albumin treated with glutaraldehyde was detected on isolated human liver parenchymal cells. however, some controversy exists regarding this hypothesis, because a receptor for formaldehyde-treated bovine serum albumin (f-bsa) has been found on liver non-parenchymal cells. therefore, we characterized the uptake of pol ... | 1992 | 1323561 |
| enhanced expression of hla class i by inhibited replication of hepatitis b virus. | hla class i display on hepatitis b virus (hbv)-infected hepatocytes is important for limiting hbv infection. however, the effect of hbv replication on hla class i expression on host cells has not been determined. since acyclovir is known to inhibit hbv replication of the novel cell line hb611, which was transfected with hbv genome using human hepatoblastoma cells as the recipient and continuously replicates hbv dna, we analyzed hla class i expression on acyclovir-treated hb611 by quantitative fl ... | 1992 | 1323597 |
| interferon-gamma receptor expression in chronic hepatitis b virus infection. | it is known that interferon-gamma (ifn gamma) is not effective in inducing a sustained inhibition of hbv replication in patients with chronic hbv infection in contrast to interferon-alpha (ifn alpha). to determine whether this was related to ifn gamma receptor (ifn gamma-r) underexpression, binding characteristics of ifn gamma to peripheral blood lymphocytes were studied in chronic hbv infection using radioiodinated recombinant ifn gamma. peripheral blood lymphocytes from patients with chronic h ... | 1992 | 1323599 |
| posttransfusion hepatitis revisited by hepatitis c antibody assays and polymerase chain reaction. | sera of 40 patients with posttransfusion non-a, non-b hepatitis were tested for hepatitis c and b viral genomes by polymerase chain reaction and for hepatitis c antibodies by synthetic peptide immunoassays. five were then considered to have chronic hepatitis before transfusion. six patients without hepatitis c markers and hepatitis b virus dna recovered. in 29 recipients who became positive for hepatitis c virus rna, posttransfusion hepatitis c was diagnosed. of them, 5 were hepatitis b surface ... | 1992 | 1321774 |
| herpes simplex virus type 2 infection, syphilis, and hepatitis b virus infection in haitian women with human immunodeficiency virus type 1 and human t lymphotropic virus type i infections. the johns hopkins university (jhu)/centre pour le developpement et la santé (cds) hiv study group. | antibodies to herpes simplex virus type 2 (hsv-2), antibodies to hepatitis b virus (hbv) core antigen (anti-hbc), and vdrl antibodies (serologic evidence of syphilis) were evaluated in women known to be infected with human immunodeficiency virus type 1 (hiv-1) (n = 95) or human t lymphotropic virus type i (htlv-i) (n = 45) and controls (n = 89). hiv-1-seropositive women were more likely than controls to have antibodies to hsv-2 (88% vs. 54%; p less than .001), anti-hbc (67% vs. 43%; p = .008), a ... | 1992 | 1321862 |
| comparison between diploid and aneuploid hepatocellular carcinomas: a flow cytometric study. | nuclear dna content of hepatocellular carcinoma (hcc) was estimated by flow cytometry after hepatic resection in 91 patients during the past 5 years. there were 53 diploid and 38 aneuploid tumours. clinicopathological features were compared retrospectively between the patients with diploid and those with aneuploid hcc. dna ploidy did not show any correlation with age, sex, alcohol abuse, hepatitis b virus, serum alpha-fetoprotein level or underlying liver disease. histopathologically, the incide ... | 1992 | 1322756 |
| the hepatitis b virus pre-s/s(t) transactivator is generated by 3' truncations within a defined region of the s gene. | recently, it was reported that 3' truncation of an integrated surface gene (pre-s2/s) cloned from a hepatitis b virus (hbv)-associated hepatoma gave rise to the generation of a c-terminally truncated middle surface protein (mhbst), which surprisingly exerted a transcriptional transactivator function. to define the molecular requirements for the generation of transactivating mhbs(t) proteins, a 3' deletion analysis of the hbv pre-s2/s gene was performed. in cotransfection experiments with reporte ... | 1992 | 1323694 |
| prevalence of markers for human immunodeficiency virus types 1 and 2, human t-lymphotropic virus type i, cytomegalovirus, and hepatitis b and c virus in multiply transfused thalassemia patients. the french study group on thalassaemia. | the prevalence of markers for human immunodeficiency virus types 1 and 2 (hiv-1, hiv-2), human t-lymphotropic virus type i (htlv-i), hepatitis b virus (hbv) and hepatitis c virus (hcv), and cytomegalovirus (cmv) was evaluated in a population of 305 multiply transfused thalassemia patients in belgium, france, and italy (sicily). no patients were found positive for hiv-2 antibodies. two french patients were seropositive for hiv-1, having been infected before systematic blood screening. antibodies ... | 1992 | 1323885 |
| prospective controlled study of post-transfusion hepatitis after cardiac surgery in a large referral hospital in india. | we studied the risk of post-transfusion hepatitis (pth) in recipients of blood collected from voluntary donors screened for hbsag. two hundred and fifty patients without any previous history of liver disease or transfusion were followed up for 12 months subsequent to cardiac surgery. thirty-five of them had closed-heart surgery without receiving transfusion and served as controls. the remaining 215 patients received single-point transfusions (mean 4 +/- 2.4 units). none of the controls and 15 (6 ... | 1992 | 1323739 |
| hepatitis c virus-related acute and chronic hepatitis in hemodialysis patients. | the incidence of hcv antibodies has been evaluated in 123 chronic hemodialysis (hd) patients (group a; 55 m and 68 f) and in 37 consecutive hd patients (group b) admitted to our hospitals for acute hepatitis. in group a, hcv antibodies were present in 27% of the patients. 20 of 36 (55%) had previously received blood transfusions. 21 patients (58%) were also positive for hbv ab. in 8 patients, alt were significantly increased. in group b, the diagnosis of hcv-related acute hepatitis was made in 1 ... | 1992 | 1323781 |
| prevalence of hepatitis c virus antibodies in chronic liver disease and hepatocellular carcinoma patients in india. | the prevalence of antibodies to hepatitis c virus (hcv) was investigated in 129 patients with chronic liver disease (85 with chronic active hepatitis and 44 with cirrhosis) and 53 patients with hepatocellular carcinoma. the commercially available second generation anti-hcv enzyme immunoassay kit was used. antibodies to hepatitis c virus were detected in 16.2% of the patients with chronic liver disease and in 15.1% with hepatocellular carcinoma. of the hcv positive patients in all groups 51.7% we ... | 1992 | 1325197 |
| transforming growth factor-alpha in human hepatocellular carcinoma and coexpression with hepatitis b surface antigen in adjacent liver. | hepatitis b virus (hbv) infection is closely associated with the development of hepatocellular carcinoma (hcc) in many patients, but the mechanisms by which hbv contributes to hcc are not known. transforming growth factor-alpha (tgf-alpha), a regulator of growth and regeneration in rat liver that can be found in high levels in some human cancers, theoretically could play such an intermediate role in the development of hcc. | 1992 | 1325266 |
| viral role in cervical and liver cancer. | hepatitis b virus (hbv) and certain human papillomaviruses (hpv) have been suspected of playing a role in human malignancy. the long latency period (decades) and the fact that only a small proportion of infected individuals subsequently have cancer suggests that these viruses probably contribute in an indirect manner to the development of cancer. analysis of viral gene function at the molecular level supports this conclusion. more studies are needed to understand the subtle interactions between ... | 1992 | 1325282 |
| rapid inactivation of infectious pathogens by chlorhexidine-coated gloves. | gloves containing chlorhexidine gluconate in an instant-release matrix on their inner surface (chg gloves) were tested to determine their ability to rapidly inactivate infectious pathogens that may permeate or leak through the latex surface. | 1992 | 1325495 |
| impact of schistosomiasis on patient and graft outcome after kidney transplantation. | in this work the impact of schistosomiasis on kidney transplantation was investigated by comparing two groups of patients, group 1 (schistosoma-infected cases) and group 2 (control cases). in group 1, schistosomiasis was diagnosed in both donor and recipient in 63 cases, in recipient only in 65 cases, and in donor only in eight cases. schistosomal infection among kidney transplant recipients was s. haematobium in 17 cases, s. mansoni in 58 cases, and mixed in 53 cases. schistosomiasis was diagno ... | 1992 | 1325622 |
| seropositivity to hepatitis c virus in saudi haemodialysis patients. | seropositivity to hepatitis b, c and d viruses was studied in 66 saudi haemodialysis patients from al baha region, south of saudi arabia, and was compared to that in 380 healthy saudis from the same region. the results showed that hcv is endemic in this region (3.6%) and the prevalence of anti-hcv in the haemodialysis patients was 45.5%, which is the highest reported so far in this group of patients. anti-hcv was related to the amount of blood transfused (greater than 5 units) and the duration o ... | 1992 | 1325714 |
| hepatitis b viruses and hepatocellular carcinoma. | | 1992 | 1325733 |
| prevalence of hepatitis b and c and hiv antibodies in children in a romanian orphanage. | two hundred and fifteen children in an orphanage in romania were examined for serum markers of present or past hepatitis b and c virus and hiv infection. in total, 183 children (85.1%) had at least one marker (hbsag, anti-hbs or anti-hbc) of hepatitis b virus infection. an hbsag carrier state was diagnosed in 38 (20.8%) of the infected children. among the carriers 24.3% were hbeag carriers, 51.4% had anti-hbe and 24.3% had neither hbe antigen nor antibody. nine children (4.2%) had antibodies to ... | 1992 | 1325807 |
| hepatocellular neoplasia in fish, rats and man: a selected comparative review. | liver cancer is a common neoplasm of man that is especially frequent in parts of the world where hepatitis b virus is endemic and high aflatoxin ingestion is experienced. hepatocellular carcinoma (hcc) has a very aggressive behavior, is quite resistant to radiotherapy and chemotherapy and is often inoperable, all of which lead to a five-year patient survival of less than 5 percent. studies in lower animals (e.g. fish, rats) lend themselves to preplanned manipulations aimed at answering specific ... | 1992 | 1325843 |
| prevalence and outcomes of hbv and anti-hcv seropositive patients with chronic liver disease and hepatocellular carcinoma. | the prevalences of serological markers of hepatitis b virus (hbv) and antibody to hepatitis c virus (anti-hcv) were determined in 168 patients (135 males and 33 females), aged 19-79 years (mean = 50.8) in thailand. of these, 33 had chronic persistent hepatitis, 35 chronic active hepatitis, 50 cirrhosis and 50 hepatocellular carcinoma (hcc). seromarkers for either hbv or anti-hcv or both were detected in 140 (83.3%), 3 (1.8%) and 18 (10.7%) patients, respectively, but 7 (4.2%) were sero-negative ... | 1992 | 1326124 |
| increased growth of permanent mouse fibroblasts in soft agar after transfection with hepatitis b virus dna. | previously we have shown that a nontumorigenic mouse hepatocyte line harboring simian virus 40 large tumor antigen (sv 40 tag) could be converted to a full-malignant phenotype by transfection with hbv dna. using a permanent sv 40 tag-negative mouse fibroblast cell line (ltk-), we studied whether the in vitro-oncogenicity of hbv was dependent on simultaneous expression of sv 40 tag or not. three fibroblast lines stably transfected by full-length hbv dna formed four times more colonies of large si ... | 1992 | 1326258 |
| viruses in human tumors--personal reflections. | a personal view is presented reflecting some developments in human tumor virology during the past three decades. since the discovery of the epstein-barr virus in 1964 members of additional viral groups have been linked to human tumors, e.g. hepatitis b virus, htlv-1, and a number of different papillomavirus types. it became evident that the virus infection per se is not sufficient and that additional events modifying the host cell genome contribute to the development of virus-linked human cancer ... | 1992 | 1326272 |
| [recurrence of virus b hepatitis after liver transplantation]. | thirty-three hbs antigen positive patients without signs of viral replication underwent orthotopic liver transplantation and received long term passive immunoprophylaxis with anti-hbs immunoglobulins at high doses perioperatively and then at a dose of 10,000 iu every month. all patients became negative during the first 6 months following surgery. at 34 months the survival rate was 67 percent and the actuarial recurrence rate of serum hbs antigen was 7.1 percent. reappearance of hbs antigen was a ... | 1992 | 1326456 |
| [enzymatic amplification of sequences of viral nucleic acids. a methodological revolution promising new prospects in hepatology]. | | 1992 | 1326457 |
| [abnormal expression of hepatitis b virus sequences integrated in human hepatocellular carcinomas]. | although epidemiologic studies have clearly demonstrated the importance of the hepatitis b virus in the genesis of hepatocellular carcinoma, the molecular basis for this tumorigenic effect is still under debate. the finding of hepatitis b virus dna integration into human liver dna in many cases of hepatocellular carcinoma suggested that these integrated viral sequences may be involved in liver carcinogenesis. in an attempt to clarify this point, we studied 9 tumors which developed in non cirrhot ... | 1992 | 1326460 |
| hepadnavirus integration: mechanisms of activation of the n-myc2 retrotransposon in woodchuck liver tumors. | in persistent hepadnavirus infections, a distinctive feature of woodchuck hepatitis virus (whv) is the coupling of frequent viral integrations into myc family genes with the rapid onset of primary liver tumors. we have investigated the patterns of whv dna insertion into n-myc2, a newly identified retroposed oncogene, in woodchuck hepatomas resulting from either natural or experimental infections. in both cases, integrated viral sequences were preferentially associated with the n-myc2 locus. in m ... | 1992 | 1323693 |
| prevalence of markers of hepatotropic viruses among drug addicts in warsaw, poland. | we studied 100 unselected parenteral drug abusers for infection with hepatitis c, b, a and d virus (hcv, hbv, hav and hdv). seventy-six percent had serological evidence of hcv infection. 12% were positive for hbsag and at least one marker of hbv infection was present in 69%. these results were significantly higher than in a matched control population. compared to controls, the prevalence of anti-hav (65%) was not significantly increased in drug addicts. of the anti-hcv-positive drug addicts, 80. ... | 1992 | 1324268 |
| clinical and laboratory presentation of lichen planus patients with chronic liver disease. | a recent case-control study on 577 lichen planus (lp) patients and 1008 controls confirmed that lp patients may significantly associate with a chronic liver disease (cld) which is independent from drug or alcohol intake and has some connection with hepatitis b virus (hbv) infection. the study, however, failed to define the nature of cld. this has been investigated through the clinical and laboratory features of 50 patients with lp and impaired liver function tests. overall, the laboratory signs ... | 1992 | 1327093 |
| validation of virus inactivation by heat treatment in the manufacture of diaspirin crosslinked hemoglobin. | diaspirin crosslinked hemoglobin (dclhb), a hemoglobin based oxygen carrying solution prepared from outdated human blood, is subjected to a heat treatment step to inactivate viruses in our manufacturing process. to validate the efficacy of this inactivation, we have simulated the heat treatment procedure at a reduced scale using hemoglobin solution spiked with representative viruses. human immuno-deficiency virus (hiv), cytomegalovirus (cmv), and duck hepatitis b virus (dhbv) were used in this v ... | 1992 | 1327242 |
| prevalence of markers of hepatitis viruses in out-patient alcoholics. | the prevalence of hcv, hbv and hav markers was investigated in unselected patients attending an outpatient alcoholic clinic. anti-hcv were detected in 35 (24%) of 144 patients studied, and at least one marker of hbv infection was present in 72 (50%). these results are significantly higher than in a matched control population. the presence of anti-hcv was related to previous blood transfusions and familial history of alcoholism. we conclude that alcoholics should be considered a high risk group f ... | 1992 | 1324269 |
| mutations on free and integrated hepatitis b virus dna in a hepatocellular carcinoma: footprints of homologous recombination. | hepatitis b virus nucleotide sequences derived from a hepatocellular carcinoma with free and multiply integrated viral dnas were determined. based on a comparison within the x-gene region, cloned free viral dna previously had been attributed to two distinct groups of prec minus genomes. the comparison of the complete sequence identified one of the genome equivalents as a recombinant between members of these groups. four different integrated viral dna elements were cloned and analysed. similarity ... | 1992 | 1326735 |