lymphoblastoid cell lines from sarcoid patients. 1978206399
athymic nude mice: induction of tumors containing epstein-barr virus using burkitt's-related cell lines.we studied tumor induction in athymic nude mice by d98/hr-1 cells, an epithelial somatic cell hybrid containing the epstein-barr virus (ebv) genome, and by the parental d98 and hr-1 cells. groups of animals were inoculated with cells grown in culture, with cells from tumors induced by the cell lines, or with cells from lines derived from tumors. the tumors induced by d98/hr-1 cells were undifferentiated carcinomas; those induced by d98 cells were carcinomas and those induced by hr-1 cells were p ...1978206453
lymphocytes transformed by epstein-barr virus. induction of nuclear antigen reactive with antibody in rheumatoid arthritis.sera from approximately two-thirds of patients with rheumatoid arthritis contain an antibody which is reactive with a nuclear antigen present in human b-lymphocyte tissue culture cells. the immunological reaction can be demonstrated by precipitation and immunofluorescence. evidence is present that the reactive nuclear antigen is associated with epstein-barr (eb) virus-transformed lymphocytes. normal human peripheral blood lymphocytes did not contain the nuclear antigen reactive with rheumatoid a ...1978206643
sensitization of lymphocytes against pooled allogeneic cells. i. generation of cytotoxicity against autologous human lymphoblastoid cell lines.lymphocytes sensitized in vitro to a pool of x-irradiated allogeneic normal lymphocytes from 20 individuals develop cytotoxic activity for autologous human lymphoblastoid cells (lcl). whereas pool sensitized t lymphocytes lyse autologous lcl cells, they fail to lyse autologous b-enriched or t-enriched normal target cells nor autologous phytohemagglutinin (pha) blasts. in contrast to pool sensitization, stimulation with normal cells of single allogeneic individuals rarely led to development of cy ...1978206649
expression of an antigen associated with acute lymphoblastic leukemia in human leukemia-lymphoma cell lines. 1978206705
epstein-barr virus-associated thymidine kinase.superinfection of raji cells with epstein-barr virus induced a new thymidine kinase that was distinguishable from both adult and fetal kinases of the host cell by discontinuous electrophoresis on polyacrylamide gels and glycerol gradients.1978206728
selected observations on the epidemiology of pharyngeal cancers.the epidemiology of nasopharyngeal cancer (npc) has been compared with that of other pharyngeal cancers in los angeles county. epstein-barr virus (ebv) antibody titers were elevated in caucasians with pharyngeal squamous cell carcinomas regardless of subsite. as expected, an excess of npc cases was found among chinese. the role of various etiologic factors in npc including ebv, inhaled carcinogens, and salted fish was discussed.1977206840
cleavage of epstein-barr virus dna by restriction endonucleases ecori, hindiii and bami.the cleavage of the dnas of the b95-8 and p3hr-1 virus strains of epstein-barr virus by the restriction endonucleases ecori, hindiii and bami was investigated using a new technique for quantitative evaluation of the fluorescence of ethidium stained dna fragments separated on agarose gels. the results obtained with b95-8 dna showed that in addition to the limited repetitions of nucleotide sequences observed in the ecori and hindiii cleavage patterns, the molecule contained a bami fragment with a ...1978206875
establishment of an epitheloid cell line and a fusiform cell line from a patient with nasopharyngeal epithelioid cell line and a fusiform cell line were established from a tumor biopsy from a patient with nasopharyngeal carcinoma which as histologically diagnosed as a well differentiated squamous cell carcinoma. based on studies of the cell growth pattern, chromosome analysis, heterotransplantation, and electron microscopy, these two cell lines were considered to be squamous carcinoma cells, and the fusiform cells might have originated from the epithelioid cells. there were many round cells ...1978206962
genetics of neoplasia--impact of ecogenetics on oncogenesis. a review. 1978207190
long-term cultivation of plasma cell leukemia cells and autologous lymphoblasts (lcl) in vitro: a comparative study.two long-term cell lines were established in vitro from the peripheral blood of a patient with plasma cell leukemia: one line with plasma cell proliferation, the other with lymphoblastoid cell proliferation (lcl). the 9-month-old plasma cell line showed the typical morphology of plasmoblasts. the cells neither had b- nor t-lymphocyte characterisitics, were ebv negative, and showed aneuploidy with various marker chromosomes, including the 14 q+ marker. the cytogenetic findings indicate a monoclon ...1978207377
non-endemic burkitt's lymphoma. 1978207381
shedding of anti-igm from antibody-coated human lymphoma lines: differences between epstein-barr virus-negative lines and their virus-converted vitro converted, epstein-barr (ebv) genome-carrying cell lines showed a reduced ability to shed surface-bound antibodies, compared to their ebv-free parental b lymphoma lines. on the other hand, antibody degradation was higher with ebv-positive cells. analysis of antibody-coated single cells revealed that cells which failed to redistribute or cap their ligand-bound surface components at 37 degrees c, were also capable of shedding bound antibody. this suggests that capping and shedding are ess ...1978207534
[sero-epidemiologic studies of epstein-barr virus in israel]. 1978207624
relationship between epstein-barr virus (ebv)-production and the loss of the ebv receptor/complement receptor complex in a series of sublines derived from the same original burkitt's lymphoma.virus production, ebv (p3hr-1 substrain) superinfectability, idurd inducibility, ebv receptor and complement (c3) receptor expression were assessed in two independently maintained jijoye lines, the derived p3hr-1 clone that releases a growth inhibitory and cytopathic, non-transforming viral mutant, and in non-producer sublines derived from the p3hr-1 line by the spontaneous cessation of virus production. both jijoye lines were superinfectable, inducible, and carried ebv and c3 receptors. virus-p ...1978207646
demonstration of a surface antigen on epstein-barr virus genome-carrying lymphoid cells: distinction from the virus-determined membrane antigen.a surface antigen (sa) was detected on epstein-barr virus (ebv)-carrying lymphoid cell lines by indirect membrane immunofluorescence with an antiserum from a rabbit immunized with raji cells; the antiserum had been extensively absorbed with normal human blood and tonsil cells. the sa was not detected on normal human umbilical cord and adult peripheral blood lymphocytes or ebv-negative cell lines. incidences of the sa and ebv-determined membrane antigen (ma) on certain ebv-carrying cell lines wer ...1978207776
reversible inhibition by phosphonoacetic acid of human b lymphocyte transformation by epstein-barr virus. 1978208257
mechanisms of infection with epstein-barr virus. iii. the synthesis of proteins in superinfected raji cells. 1978208266
detection of epstein-barr virus (ebv) dna sequences using in situ situ hybridization was used to detect epstein-barr virus (ebv) dna sequences under conditions where the virus dna is replicating spontaneously and where it is induced to do so following superinfection. the in situ reaction itself is influenced by several parameters, analogous to conventional nucleic acid hybridization, consideration of which should help to optimize the designing of in situ hybridization reactions in general. both ebv complementary rna (crna) and ebv dna synthesized in vitro c ...1978208612
epstein-barr virus. is it timely to discuss a vaccine? 1978208671
the epstein-barr virus (ebv) in human pathology. ii. serologic profiles of ebv infections.antibodies to ebv induced intracellular antigens, (vca, ea, na) and vca-igm antibodies have been investigated to define ebv serologic profile of 245 individuals. this profile was a first studied in ebv primary infections. in infectious mononucleosis, the efficiency of ebv serodiagnosis is lower than paul bunnel davidsohn reaction (pbd) : primary infection profile is only characterised in 80% of the positive pbd infectious mononucleosis (im). on the other hand, ebv antibodies are preponderant for ...1978208675
experimental infection of callithrix jacchus marmosets with herpesvirus ateles, herpesvirus saimiri, and epstein barr virus.we inoculated common marmosets (callithrix jacchus) with herpesvirus ateles (hva), herpesvirus saimiri (hvs), and epstein-barr virus (ebv). hva-induced tumors contained several cell types, including giant cells reminiscent of the sternberg-reed cells observed in human hodgkin's disease. hvs and ebv did not induce tumors, although hvs was present in lymphocytes and elicited a strong antibody response. ebv elicited only a variable antibody response. we feel that more common marmosets should be use ...1978208678
human studies following models of tumorigenesis by dna tumor viruses in animals.the discovery of virus-specific messenger rna in virus-induced animal tumors has led to the search for messenger rna in human tumors that can be hybridized with the dna of known oncogenic viruses. attention has focused on the adenoviruses, which have produced cancer in laboratory animals and are widespread in man, and on three papovaviruses that have been isolated in human disease and which are oncogenic in hamsters. in other research, the association between human infection with herpesivurs typ ...1978208690
production of migration inhibitory factor (mif) by human leukocytes following exposure to epstein-barr virus. 1978208782
stimulation of peripheral human lymphocytes by autologous ebv genome-carrying lymphoblastoid cell lines. 1978208783
[relations between malaria and burkitt's lymphoma].malaria, especially forest malaria, is the environmental factor which creates endemic conditions for the esptein-barr virus related burkitt's lymphoma. the plasmodium seems active through a mitogenic factor rather than through the so often invoked immunological depresssion.1977208788
epstein-barr virus-positive japanese burkitt lymphoma. 1978208908
somatic cell hybrids between human lymphoma lines. iv. establishment and characterization of a p3hr-1/daudi hybrid. 1978208988
further characterization of a herpesvirus-positive orang-utan cell line and comparative aspects of in vitro transformation with lymphotropic old world primate orang-utan (pongo pygmaeus) suspension line, cp81, was shown to lack myeloid markers of lysozyme activity an d phagocytosis but to be positive for lymphocytic n-alkaline phosphatase activity, and to release a b-cell-tropic herpesvirus. this herpesvirus, termed herpesvirus pongo, had 30--40% dna homology with ebv and was present at 2-3 genome copies per cp-81 cell. gibbon lymphocytes transformed by h. pongo, epstein-barr virus (ebv), and h. papio (of baboon, papio hamadryas, origin) were found ...1978208990
inhibition of dna metabolism in human b lymphocytes by a substrain of epstein-barr virus (p3hr-1): a method for virus quantitation.pretreatment with the p3hr-1 substrain of epstein-barr virus (ebv) inhibited approximately 85% of the dna stimulation induced by staphylococcus aureus in human b lymphocytes. in parallel experiments, the dna stimulation induced by the transforming b95-8 substrain of ebv was almost completely inhibited by prior exposure to p3hr-1 virus. phytohemagglutinin stimulation was only slightly inhibited, whereas the dna synthesis of continuously growing raji cells was inhibited to a considerable extent. t ...1978208998
polyploidization of epstein-barr virus (ebv)-carrying lymphoma lines decreases the inducibility of ebv-determined early antigen following p3hr-1 virus superinfection or iododeoxyuridine treatment.four tetraploid cell lines were derived from the epstein-barr virus (ebv)-carrying, nonproducer burkitt lymphoma line raji after colcemide treatment. ebv early antigen (ea) was induced by p3hr-1 virus superinfection and by iododeoxyuridine treatment. inducibility was reduced in all tetraploid lines compared to the near-diploid counterpart. the average number of ebv genome equivalents per tetraploid cell was 60-70, compared to 50 in diploid cells. this contrasts with the ebv genome amplification ...1978209000
studies on the association of the epstein-barr virus genome and human chromosomes.we have used human-mouse somatic cell hybrid to study the association between the ebv genome and the cellular genome. attempts were made to identify a specific human chromosome(s) with which the ebv genome is associated. our data suggest that at least in the mouse fibroblast/burkitt lymphoma hybrid combination studies, the ebv genome is not associated with any specific human chromosome.1978209053
bone marrow-derived lymphoid cell lines from patients with vitro infection with epstein-barr virus of bone marrow-derived (b) lymphocytes from blood of patients with x-linked or common varied agammaglobulinemia resulted in the establishment of long-term b-lymphoid cell lines (lcl). lcl were established only with b lymphocytes from patients whose b cells failed to respond to in vitro mitogenic stimulation. in contrast, lymphocytes from patients without small b lymphocytes or with b cells that synthesized but failed to secrete ig failed uniformly to es ...1978209061
epstein-barr virus infections in brazil. ii. hodgkin's disease.sixty-seven cases of hodgkin's disease (hd) occurring in são paulo, brazil, were studied. males with hd predominated over females 2.3 to 1. sixty-six percent of the cases occurred in patients under 30 years of age, 31.7% under 20 years of age, and only 7.5% after 50 years of age. lymphocyte predominance and mixed cellularity histologic types were most common in patients less than 15 years old, and nodular sclerosis was most common in the 15- to 19-year-old group. sera from all patients had antib ...1978209202
[isolation of the epstein-barr herpes virus from a lymphoblastoid cell culture]. 1978209442
restricted classes of immunoglobulin produced by a lymphoid cell line from a patient with agammaglobulinemia.restricted expression of immunoglobulin by a long-term b lymphoid cell line derived from a patient with x-linked agammaglobulinemia is reproted. the patient had peripheral blood b lymphocytes with surface igd and igm. culture of the b cells in vitro with mitogens did not stimulate immunoglobulin secretion, although pokeweed mitogen stimulation resulted in the development of cytoplasmic igd and igm. the lymphoid cell line established from these b lymphocytes primarily bore surface igd, with a sma ...1978209464
b-cell nature malignant lymphoma probably caused by eb-virus cells in a haemopericardium associated with a b-cell malignant lymphoma, immature herpes type virus particles were found by electron microscopy. epstein-barr virus associated nuclear antigen (ebna) and virus capsid antigen (vca) were also found, both in the tumor cells, in the bloody pericardial fluid and in cultivated cells. serological studies revealed high anti-toxoplasma antibody levels both in the pericardial fluid and in serum. both eb virus and toxoplasma infections are assumed to have ...1978209610
stimulation of antibodies to epstein-barr virus (ebv) in acute viral infections.acute and convalescent sera from 77 patients with serologically confirmed influenza, measles and adenovirus infections and from 36 healthy controls were tested for the level of antibodies to epstein-barr (eb) virus. in the three groups of patients significantly higher titers of antibodies to eb viral capsid antigen (vca) were found as compared to the controls. in 19 patients twofold or higher rise in antibody titers between the first and second blood sample was demonstrated. it is suggested tha ...1978209767
epstein-barr virus-associated antigens in nasopharyngeal carcinoma.thirty-five sera from american patients with nasopharyngeal carcinoma were examined for epstein-barr virus (ebv)-associated antigens and compared with 85 sera from patients with other head and neck cancers, 80 sera from patients with lymphoma, and 47 sera from healthy control subjects. there was a definite correlation between the presence of nasopharyngeal carcinoma and the level of antibody titers to ebv. in particular, two tests that detected antibody to early antigen and antibody to viral cap ...1978209772
a new manifestation of infection with epstein-barr virus. 1978209846
cultural, morphological, cell membrane, enzymatic, and neoplastic properties of cell lines derived from a hodgkin's disease lymph node.a neoplastic cell line (designated hut11) has been established in continuous culture from an involved lymph node of a patient with stage iia hodgkin's disease of the mixed cellularity type. the hut11 line has been morphologically heterogeneous, consisting of mononucleate lymphoid-like cells, polygonal epithelioid cells, and mono-, bi-, and multinucleate giant cells. four clones initiated from isolated binucleate giant cells of the hut11 line also have been successfully established as continuous ...1978209894
detection of epstein-barr virus in biopsied malignant lymphoma cell and its continuous more than 80% of tumor cells in the pericardiac effusion of a case of malignant b-cell lymphoma, epstein-barr virus-determined nuclear antigen (ebna) was detected by the anticomplement immunofluorescence test. moreover, herpestype virus particles, although few in number, were demonstrated in the nucleus of lymphoma cells by an electron microscope. tumor cells in the pericardiac effusion were seeded at 98% purity after centrifugation on ficoll-conray and, soon after plating, they proliferated ...1978210075
antigen-inducing ability of herpesvirus papio in human and baboon lymphoma lines, compared to epstein-barr virus.herpesvirus papio(hvp)-carrying baboon lymphoblastoid lines do not express a nuclear antigen like the epstein-barr virus(ebv)-determined nuclear antigen (ebna), as judged by in situ anticomplement fluorescence staining, although the carry multiple viral genomes and, in the case of producerlines, early antigen (ea) and viral capsid antigen (vca) that cross-react with the corresponding human ebv-determined antigens. to test whether the lack of in situ nuclear antigen expression is a property innat ...1978210135
isolation of a heterogeneous population of temperature-sensitive mutants of measles virus from persistently infected human lymphoblastoid cell lines.two human lymphoblastoid b-cell lines, wi-l2 and 8866, were infected with the edmonston strain of measles virus at a multiplicity of infection of 10(-6), and stable persistent infections were established. by immunofluorescence and electron microscopy, the vast majority of cells from both cell lines were expressing viral antigens and releasing virion-like particles. however, very little infectious virus could be detected at 37 degrees c, either by an infectious centers assay or by titration of su ...1978210247
transfusion-related short-incubation hepatitis in hemophilic patients.nine episodes of a unique short-incubation form of hepatitis were observed during five years in six hemophilic children after infusion with commercial factor viii concentrate prepared by two different manufacturers. five patients with a single episode had no previous infusion for 14 months to 14 years. one patient with several episodes had no previous infusion for at least seven months preceding each episode. the illness was mild and self-limited. no seroconversions to cytomegalovirus, epstein-b ...1978210302
epstein-barr virus as the cause of a human cancer. 1978210391
epidemiological evidence for causal relationship between epstein-barr virus and burkitt's lymphoma from ugandan prospective study.results from a prospective sero-epidemiological study initiated in uganda in 1971 indicate that children with high antibody titres to epstein-barr virus structural antigens are at high risk of developing burkitt's lymphoma. these findings strongly support a causal relationship between the epstein-barr virus and burkitt's lymphoma but suggest that the oncogenic potential of the virus is realised only in exceptional circumstances.1978210392
binding to chromosomes of herpes simplex-related antigens in biochemically transformed cells.association of herpes simplex virus (hsv)-related antigens with chromosomes was demonstrated in human and mouse cells biochemically transformed by hsv that had been irradiated with ultraviolet light. this was accomplished by using peroxidase-anti-peroxidase immunological staining with rabbit antisera that had high neutralizing titers against both hsv-specific thymidine kinase activity and virus infectivity. antisera-against hsv did not react with chromosomes of uninfected cells nor did normal se ...1978210457
epstein-barr virus antibodies and histocompatibility type in multiple sclerosis. 1977210555
expression of altered ribonucleotide reductase activity associated with the replication of the epstein-barr virus. 1978210572
current assessment of "case clustering" of lymphomas and leukemias. 1978210923
host cell reactivation of epstein-barr virus in normal and repair-defective leukocytes. 1978210932
a greatly simplified method of establishing b-lymphoblastoid cell lines.ten lymphoblastoid cell lines were established by epstein-barr virus-induced transformation directly from 0.04 to 0.15 ml of peripheral whole blood of one patient with xeroderma pigmentosum and four normal healthy adults. all these lines expressed b-lymphocyte characteristics. the advantages of this method are: (a) only a few drops of blood are required for establishing a permanent line; (b) damage and loss of cells in separation procedures are minimal; and (c) the method is simple, reliable, an ...1978210942
clones of human lymphoblastoid cell lines producing antibody to tetanus toxoid. 1978211002
establishment of specific antibody producing human lines by antigen preselection and ebv-transformation. 1978211003
infection of taiwan monkey t and b cells with epstein-barr order to establish taiwan monkey lymphoblastoid cell lines, attempts were made to raise the susceptibility of monkey lymphocytes to epstein-barr virus (ebv) by chemical and enzymatic treatments. ebv infectivity to monkey t and b cells were tested by detection of ebv early antigens in infected cells with the indirect immunofluorescent antibody technique. treatments of monkey unfractionated lymphocytes with deae-dextran (160 microgram/ml) for 1 hr, ethylenediamine tetra-acetic acid (edta, 0.5%) ...1977211014
igg and iga antibodies to epstein-barr virus in nasopharyngeal carcinoma patients.immunoglobulin levels and igg and iga antibodies to epstein-barr virus (ebv), viral capsid antigens (vca) and early antigens (ea) were tested in 67 untreated (5 stage i, 32 stage ii, 16 stage iii and 14 stage iv), 21 treated and 7 recurrent nasopharyngeal carcinoma (npc) patients and 54 normal subjects. the mean serum concentrations of igg, iga and igm in npc patients were higher than those of the normal control. 41.1, 86.3, 94.7 and 95.8% of npc patients had anti-vca titers of greater than or e ...1978211015
activation of the alternative complement pathway by human b cell lymphoma lines is associated with epstein-barr virus transformation of the cells.activation of the alternative complement pathway by human b cell lymphoma lines is correlated with the presence of epstein barr virus (ebv) in the cell genome. ebv-negative b cell lymphoma lines produce little activation of the alternative pathway as measured either by c3 deposition on the cell surface or c3 conversion and consumption of alternative pathway activity in the supernatant serum. by contrast, ebv-positive sublines derived by in vitro ebv conversion of ebv-negative parental lines prod ...1978211040
sensitivity of the transforming and replicative functions of epstein--barr virus to inhibition by phosphonoacetate.disodium, phosphonoacetate (pa), at concentrations of 50 to 200 microgram/ml, which still allowed continued growth of the eb virus-transformed b95-8 cell line on a routine culture regimen, was able to inhibit the production of virus capsid antigen and of virus particles by these cells down to very low but finite levels which persisted despite prolonged treatment. further experiments measured the effects of these same drug concentrations on the eb virus-induced in vitro transformation of foetal c ...1978211188
improved detection of virus-specific igm antibodies. elimination of non-specific igm binding.a non-specific reaction between human igm and cytoplasmic structures of virus infected cells can often be observed if mgm antibodies to virus antigens are detected by indirect immunofluorescence or by immuno enzyme assays. formaldehyde selectively inactivates the cytoplasmic receptors for human igm without affecting the virus structural proteins. alternatively, receptor-free antigens can be obtained by isolation of nuclei from virus infected cells. due to reduced background, a more specific and ...1978211190
studies of epstein-barr virus (ebv)-associated nuclear antigen: solubilization from raji cell chromatin with 5 m-urea-2 m-nacl and fractionation on hydroxyapatite.ebna-containing chromatin from raji cells was solubilized by treatment with high concentrations of urea and salt and fractionated by hydroxyapatite chromatography. fractions eluting at different phosphate concentrations were analysed for the presence of ebna by means of an anti-ebna-specific 125i-labelled igg absorption assay. the antigen was found in fractions containing non-histone chromatin proteins.1978211194
persistence of both human cytomegalovirus and epstein-barr virus genomes in two human lymphoblastoid cell dna-dna reassociation kinetic analysis, less than one genome equivalent per cell of human cmv-dna was found in two lymphoblastoid cell lines, one derived from the peripheral blood of a congenitally infected male infant at the age of 21 months (d4 cell line), the other obtained by co-cultivation of lethally x-irradiated cells from the 9-month lymphoblastoid cell line previously described by joncas et al. (1975) with cord blood leukocytes of a female newborn (m1 cell line). human cmv antigens c ...1978211195
heterogeneity of epstein-barr virus. iii. comparison of a transforming and a nontransforming virus by partial denaturation mapping of their dnas.the dnas of a transforming and a nontransforming epstein-barr virus strain, b95-8 and p3hr-1, were compared by partial denturation mapping. b95-8 viral dna showed a homogeneous denaturation pattern. in contrast, p3hr-1 viral dna was heterogeneous, containing at least two classes of molecules, classified into groups a and b and present in a ratio of about 2:1 to 3:1. no evidence could be obtained that molecules from both groups a and b contain identical sequences present in different orientations ...1978211256
dna of epstein-barr virus. iii. identification of restriction enzyme fragments that contain dna sequences which differ among strains of epstein-barr virus.previous kinetic and absorption hybridization experiments had demonstrated that the dna of the b95-8 strain of epstein-barr virus was missing approximately 10% of the dna sequences present in the dna of the hr-1 strain (r.f. pritchett, s.d. hayward, and e. kieff, j. virol. 15:556-569, 1975; b. sugder, w.c. summers, and g. klein, j. virol. 18:765-775, 1976). the hr-1 strain differs from other laboratory strains, including the b95-8 and w91 strains, and from virus present in throat washings from p ...1978211267
[hodgkin's disease: effect of spenectomy on the immune status (author's transl)].in 22 untreated hodgkin's patients the following parameters were studied before and after splenectomy: the total wbc, lymphocytes, b- and t-cells and mitogenic stimulation of peripheral lymphocytes using autologous and control serum. the results were correlated to patient groups with favourable and unfavourable prognosis according to pathological stage, histology, spleen involvement and constitutional symptoms. 1. all patients. significant increase of the absolute number of peripheral lymphocyte ...1978211317
chloroquine enhances epstein-barr virus expression. 1978211434
[[auto] critique of a tumor virology (author's transl)]. 1978211545
[incidence and specificity of circulating immune complexes in infectious mononucleosis (proceedings)].occurrence of immune complexes in infectious mononucleosis has been investigated by the 125i clq binding assay. increased serum clq-binding activity was found in 87% of the 23 patients studied during the acute stage of the disease. the serum clq-binding material detected has properties identical to those of immune complexes. igg antibodies dissociated from the complexes at acid ph and f (ab)'2 fragments obtained after treatment by pepsin appeared to be directed against the viral capsid antigen o ...1978211580
alkaline phosphatase in epstein-barr viral nuclear antigen--positive cell lines.the production and nature of alkaline phosphatase were studied in epstein-barr viral nuclear antigen-positive, surface membrane immunoglobulin negative-cell lines established from two patients, one with acute myeloid leukemia and one with acute lymphoblastic leukemia. the acute myeloid leukemia-derived cells contained myeloid alkaline phosphatase, while the acute lymphoblastic leukemia-derived cells contained lymphoid alkaline phosphatase. the presence of the myeloid-specific enzyme in a surface ...1978211588
human malignant lymphomas in vitro. characterization of biopsy cells and establishment of permanent cell lines.a series of 55 biopsies from different types of malignant lymphomas were characterized in short-term culture experiments and during prolonged growth in vitro. the majority of the lymphocytic lymphomas and half of the histiocytic lymphomas expressed surface immunoglobulin, either in monoclonal or polyclonal form, indicating b-lymphocyte derivation. no lysozyme production was noted in either type of lymphoma, giving further support to the notion that histiocytic lymphomas are not truly histiocytic ...1978211794
infection with epstein-barr virus. 1978212156
the values of epstein-barr virus titers as a diagnostic shortcut in infectious mononucleosis: two case reports. 1978212244
antibody-dependent cellular cytotoxicity against epstein-barr virus-associated antigens in african patients with nasopharyngeal carcinoma.antibody-dependent cellular cytotoxicity (adcc) was performed on serum samples from african patients with nasopharyngeal carcinoma (npc) to determine whether antibody titers determined by this assay might be of prognostic value in this disease. the serum donors were divided into two groups: (1) those individuals who died within 2 years following diagnosis of npc; and (2) individuals who responded well to therapy and surivived for more than 2 years following diagnosis. the adcc gmt for the surviv ...1978212366
antibody patterns to herpesviruses in kaposi's sarcoma. ii. serological association of american kaposi's sarcoma with cytomegalovirus.the prominent finding of this extended serologic analysis on american and african kaposi's sarcoma (ks) patients and appropriately matched control groups is the detection of a specific serologic association of cytomegalovirus (cmv) with american ks patients. all american ks sera contained cmv antibodies and their geometric mean titers (gmt) were significantly higher than those in sera of melanoma patients (gmt ratio k = 5.3 to 7.7 by complement fixation [cf], k = 8.9 by indirect hemagglutination ...1978212367
primary epstein-barr virus infections in african infants. i. decline of maternal antibodies and time of infection. 1978212369
primary epstein-barr virus infections in african infants. ii. clinical and serological observations during seroconversion. 1978212370
interaction of the b95-8 and p3hr-1 substrains of epstein-barr virus (ebv) with peripheral human lymphocytes.two substrains of the epstein-barr virus derived from the b95-8 and p3hr-1 cell lines were studied for their interaction with human peripheral lymphocytes. it has been previously shown that b95-8 virus has and p3hr-1 virus lacks lymphocyte-transforming ("immortalizing") properties. dna stimulation induced by b95-8 virus showed a good correlation with the number of surface ig-positive cells. p3hr-1 virus added before b95-8 virus completely abolished the stimulation of dna synthesis. it also preve ...1978212371
presence of ebna in nasopharyngeal carcinoma and control patient tissues related to ebv serology. 1978212372
recovery of transforming ebv from non-producer cells after superinfection with non-transforming p3hr-1 ebv.cells of the raji and nc37 lines can be induced by chemical inducers, such as brdurd and idurd, or the tumor-promoter tpa to ea-expression only, but do not reveal any vca synthesis. after superinfection by nontransforming p3hr-1 ebv, however, a varying percentage of the cell population shows vca synthesis and releases infectious viral particles. the recovered virus differs biologically from p3hr-1 ebv since it transforms human umbilical cord blood lymphocytes into ebna-positive lymphoblastoid ce ...1978212377
detection of epstein-barr virus antigens and antibodies by peroxidase-labeled specific immunoglobulins.detection of the epstein-barr (ebv) antigens, early antigen (ea), viral capsid antigen (vca), and nuclear antigen (ebna) by the indirect immunoperoxidase technique was highly sensitive. antibody titers to ebna, ea, and vca were determined in more than 25 sera of patients with burkitt lymphoma (bl), nasopharyngeal carcinoma (npc), or normal persons. a good correlation between the titers of these antigens was obtained by the immunoperoxidase and immunofluorescence methods. the indirect (anti-igg) ...1978212523
effects of adenine arabinoside on lymphocytes infected with epstein-barr virus.low concentrations of adenine arabinoside inhibited growth of two epstein-barr virus producer cell lines in culture, while not significantly affecting a nonproducer cell line and a b-cell-negative line. these observations were extended to include freshly infected cells. mitogen-stimulated human umbilical cord blood lymphocytes were unaffected by the drug at concentration levels that inhibited [3h]thymidine incorporation into the dna of epstein-barr virus-stimulated cells. dna synthesis in epstei ...1978212577
purification of the epstein-barr virus-determined nuclear antigen from epstein-barr virus-transformed human lymphoid cell lines.the epstein-barr virus-determined nuclear antigen (ebna) was purified from extracts of the human lymphoid cell lines raji, namalwa, and b95-8/mld by two different methods. in the first approach, the apparently native antigen was purified 1,200-fold by a four-step procedure involving dna-cellulose chromatography, blue dexptran-agarose chromatography, hydroxyapatite chromatography, and gel filtration, employing complement fixation as the assay procedure. such ebna preparations specifically inhibit ...1978212583
promising new antiherpes agent being tested in humans. 1978212617
[virus diseases of the lymphatic system. differential diagnosis and therapy]. 1978212661
chromosomal association of an epstein-barr virus-associated nuclear antigen. 1978212669
[complex antibody studies in suspected infectious mononucleosis]. 1978212703
isolation of a herpesvirus-specific dna polymerase from tissues of an american patient with burkitt lymphoma.a dna polymerase (dna nucleotidyltransferase) has been partially purified from a neck mass of an american patient with burkitt lymphoma and separated from the cellular dna polymerases. the molecular weight of the enzyme was approximately 90,000. the enzyme differs from the cellular dna polymerases, but resembles herpes-virus-induced dna polymerase in its primer template preference, high monovalent cation requirement for activity, and sensitivity to phosphonoacetate. enzyme activity was inhibited ...1978212756
activation of endogenous type c virus in balb/c mouse cells by herpesvirus dna.several virion and nonvirion dnas were tested for the ability to activate endogenous type c virus in balb/c-derived mouse cells using the calcium precipitation technique. the dnas from all herpesviruses tested activated xenotropic type c virus synthesis. these included dnas from herpes simplex virus types 1 and 2, epstein-barr virus, human cytomegalovirus, sa8 virus, infectious bovine rhinotracheitis virus, pseudorabies virus, and herpes saimiri virus (m-dna). in contrast, dnas from vaccinia vir ...1978212761
prospects of vaccines against cancer in man.preventive immunization is the cheapest and most effective approach to the management of epidemic disease. certain forms of cancer, including burkitt's lymphoma, carcinoma of the cervix, primary liver cancer, acute leukaemia and breast cancer show many characteristics of epidemic disease, and the possibility of producing preventive vaccine against these conditions merits serious consideration.1978212839
[isolation of the epstein-barr virus in suspension cell lines obtained from persons with different forms of hemoblastosis].isolation of ebv from patients with different types of haemoblastosis by establishing virus-producing cell lines is described. ebv was isolated from patients with myelogenous leukemia in 71%, hodgkin's disease--in 22%. ebv was also isolated from 1 patient with lung tumor and leukemogenic reaction. all 8 established cell lines produced ebv in 0.5--3% of cells. the cells of the lines concerned are lymphoblasts and show b-cell characteristics.1978212872
latent viruses and bell's palsy in children.4 children with unilateral facial palsy--bell's palsy (bp)--had serological evidence of primary infection with epstein-barr virus (ebv). concomitant infection with cytomegalovirus (cmv) was demonstrated in 1 child while in all 4 children a rise in antibody titers to an additional one or two viruses was demonstrated. the viruses involved were herpes simplex, cmv and adenovirus which are latent in humans. immunosuppression induced by ebv and steroid treatment may cause reactivation of these viruse ...1978213283
serological reactivities of human and baboon sera against ebna and herpesvirus papio-determined nuclear antigen (hupna). 1978213288
lysosomal acid hydrolases in established lymphoblastoid cell lines, transformed by epstein-barr virus, from patients with genetic lysosomal storage diseases.lysosomal acid hydrolases were determined in established lymphoblastoid cell lines, transformed in vitro by epstein-barr virus (ebv) from lymphocyte-rich cell populations isolated from the peripheral blood of patients with genetic lysosomal storage diseases--hurler syndrome, scheie syndrome, gm1-gangliosidosis type 1 and type 2, tay-sachs disease, and i-cell disease--and from obligate heterozygotes for these diseases. the respective enzyme activity was undectectable in lymphoblastoid cells from ...1978213379
insulin inhibits cellular and herpes simplex virus dna synthesis in transformed lymphoblastoid cell lines.treatment of uninfected and herpes simplex virus-infected lymphoblastoid cells with 4 units of insulin per milliliter resulted in the inhibition of both cellular and viral dna synthesis.1978213399
two epstein-barr virus-associated dna polymerase activities.we have partially purified and characterized two separate dna polymerase activities associated with epstein-barr virus (eb virus). one activity is present in eb virus producer cell lines but not in nonproducer or negative cell lines. it adheres more strongly to deae-cellulose than any host cell enzymes, eluting at 210 to 270 mm potassium phosphate buffer. further elution from phosphocellulose and sedimentation in glycerol gradients yields an enzyme purified 900-fold with an s value of 8.3. the s ...1978213439
epstein-barr virus in somatic cell hybrids between mouse cells and human nasopharyngeal carcinoma cells.somatic cell hybrids between mouse cells and cells derived directly from npc biopsies were produced in order to study the association of the epstein-barr virus (ebv) genome and the expression of epstein-barr nuclear antigen (ebna) with the human chromosome(s). all attempts to correlate the presence of ebv-dna and the expression of ebna with the presence of a particular human chromosome(s) showed that the segregation of ebv-dna or of ebna and human chromosomes was dysconcordant. the data, therefo ...1978213442
seroconversion against epstein-barr virus in two nonhuman primate species infected by the oropharyngeal route.transforming epstein-barr virus (ebv) was inoculated into eight squirrel monkeys (saimiri sciureus) by spraying of viral concentrates into the nose and throat. virus was inoculated into three marmosets (two saguinus oedipus and one calithrix jacchus jacchus) by direct injection into nasopharyngeal tissue. two of these squirrel monkeys seroconverted after repeated inoculation as determined by the presence of ebv-specific antibodies to early antigen and viral capsid antigen. one squirrel monkey ha ...1978213505
intracisternal tubular inclusions in nasopharyngeal carcinoma.biopsy specimens obtained from 20 cases of nasopharyngeal carcinoma were examined under electronmicroscope for the presence of intracisternal tubular inclusions (iti). in eight (40 per cent) of them these inclusions could be identified. although the morphogenesis and significance of these iti are not clearly understood, they have been found in several autoimmune and viral diseases, including infectious mononucleosis, a disease caused by ebv, and burkitt's lymphoma, which is closely associated wi ...1978213517
[an autopsy case of acute myeloblastic leukemia with persistent high anti ebv-antibody titer (author's transl)]. 1978213625
screening of anti-b cell antibodies in the sera of renal transplant recipients by using daudi cell order to examine the presence of antibodies against b cells, 83 sera obtained from 55 renal transplant recipients were screened by microcytotoxicity test using daudi cell line. out of 83 samples, 12 were pretransplant sera, 62 were post-transplant sera and 9 were from the patients whose grafts has been removed due to rejection. for the purpose of improving the reliability of the tests, 5 different rabbit complements were selected and cytotoxicity tests with them were carried out simultaneousl ...1978213630
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