establishment of ebna-expressing cell lines by infection of epstein-barr virus (ebv)-genome-negative human lymphoma cells with different ebv strains.cells of two ebna (epstein-barr virus nuclear antigen)-negative human lymphoma cell lines, bjab and ramos, were infected with two strains of epstein-barr virus (ebv). in two different experiments, b95-8 virus-infected bjab cells revealed a gradually increasing number of ebna-positive cells. twenty weeks after infection almost 100% of the cell population expressed this antigen. in contrast, it has not so far been possible to convert ramos cells into an ebna-positive cell line. the initial proport ...1976175026
attempts to demonstrate virus-specific sequences in human tumors. iv. eb viral dna in european burkitt lymphoma and immunoblastic lymphadenopathy with excessive plasmacytosis.various biopsies from different european malignant lymphomas, two biopsies from nasopharyngeal carcinomas, and material from non-neoplastic lymph nodes were assayed for the presence of epstein-barr virus (ebv) dna by nucleic acid hybridization. reassociation kinetics of in vitro-labelled ebv dna were studied in the presence of tumor dna. the lymphomas tested included among others follicular lymphomas, germinocytomas, immunoblastic lymphomas and lymphoplasmacytoid immunocytomas. epstein-barr vira ...1976175027
clonal transformation of human leukocytes by epstein-barr virus in soft agar.the b95-8 strain of epstein-barr virus (ebv) induced colony formation of human umbilical cord-blood leukocytes in soft agar medium. one-hit response relationship between the number of colonies and the virus dose was observed with high dilutions of the virus preparation. however, there was a presumed cell-killing effect with low dilutions of virus. the colonies were similarly induced, but with a lower efficiency, in adult peripheral blood leukocyte cultures infected with the virus. the colony-for ...1976175028
false positive infectious mononucleosis serology in epilepsy.positive serum tests for infectious mononucleosis (im) unaccompanied by the clinical syndrome or blood changes characteristic of the disease were detected in 39/177 (22%) mentally subnormal patients investigated with three different commercially available im slide tests. slide positively was still detectable six months later in 27/35 (77%) positive reactors. positive im slide tests were significantly associated with anticonvulsant, primarily phenobarbitone therapy and with concomitant elevations ...1975175096
eb virus antibody and infectious mononucleosis in a boarding school for boys.a study of eb virus antibody was undertaken in a boarding school for boys between 11 and 18 years of age; 35% of boys had ebv antibody when first sampled and a small number acquired antibody each year. some of these boys had had no recorded illness during the period before the first positive sample and some developed classic infectious mononucleosis which was accompainied by a positive heterophil antibody test and detection of eb virus specific igm. in a school of 800 boys there were 13 cases of ...1976175098
[letters to the editor: bone marrow transplantation and leukemic transformation of transplanted cells]. 1975175191
[antibodies against the epstein-barr virus, capsid antigen in children with malignant neoplasms (author's transl)].sera from children were tested for antibodies to viral capsid antigens of epstein-barr virus. the method applied was the indirect immunofluorescence test. 25 sera from healthy donors were titrated as controls. in 14 patients with acute lymphocytic leukemia we found low levels, a tiny little girl with chronic myelocytic leukemia (?) and three children.with lymphoproliferative disorders showed highly increased serological reactivities. investigated children with other kinds of tumours did not sero ...1976175342
clinical applications of cell surface markers. 1975175398
[production of interferon or interferon-like susbstances in eb3 cultures after inoculation with human serum containing australia antigen]. 1975175431
pancytopenia and "caillary leak syndrome" with infectious mononucleosis.a 19-year-old man with documented infectious mononucleosis presented with pancytopenia and a megaloblastic bone marrow. he developed a "capillary leak syndrome" with an expanded plasma volume of 9,290 ml and normal right heart and pulmonary artery pressures. the patient had a dramatic recovery after corticosteroid therapy.1976175508
mononucleosis-associated subacute sclerosing panencephalitis.a thirteen-year-old girl died of subacute sclerosing panencephalitis (sspe) which occurred as part of a complex encephalitic illness related to acute infectious mononucleosis. the cerebrospinal fluid (csf) epstein-barr virus (ebv) fluorescent antibody (fa) titer was 1:64. electron microscopic examination revealed 17 nanometer (nm) diameter paramyxovirus-like nucleocapsids in brain sections and 90 nm diameter herpes virus-like enveloped particles in negatively stained brain tissue extracts. indir ...1976175632
human cancer viruses. 1976175647
circulating immune complexes and complement sequence activation in infectious mononucleosis.the role of immune complex formation was investigated in a patient with infectious mononucleosis complicated by an urticarial rash. circulating cryoprotein immune complexes were identified during the urticarial phase of the illness, and disappeared during recovery. these complexes were composed of immunoglobulins g (igg), m (igm) and a (iga), complement components c3, c4 and c5, epstein-barr (eb) virus capsid antibody and particles resembling eb virus. the igg subtypes identified in the immune c ...1976175655
burkitt's lymphoma - a human tumor model system for immunological studies.burkitt's lymphoma occurs mainly in parts of tropical africa and has attracted the attention of experimental workers due to its epidemiological and clinical features, which indicate a viral etiology and a host immune response to the tumor. as a result of virological studies, epstein-barr virus (ebv) dna has been demonstrated in almost all tested biopsies of african bl. this contrasts to the absence of ebv in all, or almost all, of the non-african burkitt's lymphoma-like tumors, even though the n ...1975175835
[epidemiology in the study of the role of viruses in human cancers].an epidemiological approach is very useful in the study of the role of viruses in the development of human tumors, if properly integrated with the experimental approach and laboratory studies. certain epidemiological characteristics of human tumours favour a viral etiology, e.g. space and time clustering, vertical and horizontal transmission of a tumour risk, succession in space or in time of viral induced benign lesions and of malignant tumours. in the framework of the association between the e ...1975175869
approaches to immunological control of virus-associated tumors in man: introductory remarks. 1976175916
epstein-barr virus-malaria interaction models for burkitt's lymphoma: implications for preventive trials.infections with both epstein-barr virus (ebv) and malaria have been implicated as causal factors in the pathogenesis of burkitt's lymphoma (bl). proposed trials of preventive measures for both infections are receiving serious consideration as possible means of establishing a causal relationship with bl. in this paper we examine certain models for the interaction of ebv and malaria in the induction of bl, and also review the aims of the longitudinal, population-based study being conducted in the ...1976175923
experimental models for dna-rna viral interactions: a brief review.evidences that demonstrate or imply the occurrence of dna-rna viral interactions in man and animals are reviewed. the concurrent presence of two dissimilar oncogenic viruses influenced responses both in vivo and in vitro. cell-mediated immune responses to marek's disease herpesvirus were altered in specific-pathogen-free chickens inoculated as embryos with an avian leukosis virus. the possible roles of immunological factors in the pathogenesis and control of tumor development associated with dna ...1976175924
biochemical approaches to detection of epstein-barr virus in human tumors.the application of biochemical studies for the detection of epstein-barr virus (ebv)-dna in human tumor cells is discussed. these studies resulted in the consistent demonstration of viral nucleic acid in african burkitt's lymphoma biopsies and in epithelial tumor cells of nasopharyngeal carcinomas. the viral dna resides within those cells regularly in multiple copies per cell. besides these tumors our group detected significant concentrations of ebv-dna in a german lymphoma patient revealing his ...1976175925
implications of a vaccine for the prevention of epstein-barr virus infection: ethical and logistic considerations.reasons are given for considering that there is sufficiently substantial indirect and circumstantial evidence linking epstein-barr (eb) virus to african burkitt's lymphoma (bl) and nasopharyngeal carcinoma to call for a dynamic new approach to establish a causal role for the virus in these human cancers. it would seem that the only way to do this would be to develop a vaccine, vaccinate a population at risk in a high-tumor-incidence area, and subsequently follow the population for a consequentia ...1976175931
immunological control of virus-associated tumors in man: a perspective. 1976175948
cell-killing by epstein-barr virus: analysis by colony inhibition procedure.the p3hr-1 and b95-8 strains of epstein-barr virus (ebv) were cytocidal for ebv-carrier human cell lines, as revealed by a colony inhibition procedure. the cytocidal activity was proportional to virus dose added. the cell killing was neutralized by anti-ebv antibody-positive but not -negative human sera. when the relative sensitivity to ultraviolet light of ebv activities was examined, the cytocidal actitivy was much more resistant than the viral infectivity as assayed by early antigen-forming a ...1976176118
[studies on the relationship between clinical course and antibody titer to epstein-barr virus in nasopharyngeal carcinoma (author's transl)]. 1975176338
growth in semisolid agar medium of human cord leukocytes freshly transformed by epstein-barr virus.the semisolid agar method for the selective growth of transformed cells was applied to investigations of epstein-barr virus (ebv) transformation. when 1 x 10(5) human umbilical-cord blood leukocytes were seeded in agar medium immediately after ebv exposure, about 100 colonies developed in each dish. the occurrence of colonies correlated well with dilutions of ebv inoculum. these colonies were composed of lymphoblasts, were positive for ebv-determined nuclear antigen immunofluorescence, and cons ...1976176377
workshop on epstein-barr virus: summary and recommendations. 1976176391
antibody-dependent lymphocyte cytotoxicity against cells expressing epstein-barr virus antigens.antibody-dependent lymphocyte cytotoxicity (adlc) was demonstrated against epstein-barr virus (ebv)-infected raji cells with peripheral with peripheral blood lymphocytes from ebv-infected donors. no cytotoxic activity was detected against unifected rajl cells. the results indicated that this antibody-mediated cytotoxic reaction was directed against the same ebv-induced membrane antigens (ma) previously defined by the membrane immunofluorescence (mf) assay. antibody to ebv-associated early antige ...1976176393
epstein-barr virus--is it time to develop a vaccine program? 1976176406
epstein-barr virus in an american patient with burkitt's lymphoma: detection of viral genome in tumor tissue and establishment of a tumor-derived cell line (nab).epstein-barr virus (ebv) dna (17.7 genome equivalents/cell) was found in tumor tissue from an american patient with burkitt's lymphoma who had never traveled outside the united states. a lymphoid cell line (nab) containing the ebv genome was established from tumor tissue from this patient; characteristics of this cell line were described. previous burkitt's tumors found in americans and examined by molecular hybridization were negative for ebv dna. our results suggested that ebv is associated wi ...1976176407
characterization of epstein-barr virus antigens. i. biochemical analysis of the complement-fixing soluble antigen and relationship with epstein-barr virus-associated nuclear antigen.the epstein-barr virus-soluble (s) antigen extracted from raji cells was characterized by sucrose gradient centrifugation, gel filtration, and ion-exchange chromatography. the sedimentation coefficient was estimated to be 8.5s corresponding to a molecular weight of 180,000. the s antigen binds to deae-a25 ion exchanger from which it can be eluted with 0.3 m nacl in tris buffer (ph 7.2). all fractions which contained complement-fixing s antigen also inhibited the anticomplement immunofluorescence ...1976176436
proteins of epstein-barr virus. i. analysis of the polypeptides of purified enveloped epstein-barr virus.epstein-barr virus (ebv) was purified from the extracellular fluid of hr-1 and b95-8 cell lines. the preparations of purified virus consisted of enveloped particles and had ebv-specific antigneic reactivity. comparison of the amount of labeled protein in preparations of virus purified from cultures incubated in [35s]methionine with the amount of labeled protein in preparations obtained following a mixture of unlabeled virus with [35s]methionine-labeled cellular proteins indicated that less than ...1976176452
inhibition of epstein-barr virus dna synthesis and late gene expression by phosphonoacetic acid.growth of lymphoblastoid cells (b95-8, raji) is not inhibited by the presence of 0.4 mm phosphonoacetic acid. the synthesis of epstein-barr virus (ebv) in the producer line b95-8 is completely inhibited, as shown by the total inhibition of viral capsid antigen synthesis. early viral antigens are made normally in the presence of phosphonoacetic acid, but ebv dna synthesis is blocked in cells entering the productive cycle. nonproducer cells in the population replicate the resident ebv dna by a mec ...1976176457
proteins of epstein-barr virus. ii. electrophoretic analysis of the polypeptides of the nucleocapsid and the glucosamine- and polysaccharide-containing components of enveloped virus.two series of experiments were undertaken to identify the topological location of the structural polypeptides of epstein-barr virus. in the first series of experiments, nucleocapsids prepared by detergent treatment of enveloped virus with nonidet p-40 and sodium deoxycholate were found to be composed of seven polypeptides, vp2, 6, 7.5, 24, 27, 31, and 33, which ranged in molecular weight from over 200 x 10(3) to 28 x 10(3). nine other polypeptides, vp 4, 7, 8, 10, 15, 16, 23, 28, and 29, could b ...1976176465
intracellular forms of epstein-barr virus dna in human tumour cells in vivo.tumour biopsies from burkitt lymphoma patients, as well as human nasopharyngeal carcinoma cells growing in athymic mice, contain epstein-barr virus dna as covalently closed circular dna. in addition integrated viral dna sequences seem to be present.1976176597
association of epstein-barr viral genomes with american burkitt lymphoma. 1976176599
effect of interferon on lymphocyte transformation and nuclear antigen production by epstein-barr virus. 1976176601
superinfection epithelial nasopharyngeal carcinoma cells with epstein-barr virus.attempts were made to superinfect epithelial explant cell cultures prepared from nasopharyngeal carcinomas with epstein-barr virus. virus-specific markers were observed in such cultures 3 days after superinfection. in addition, expression of epstein-barr virus early antigens was observed in epithelial cell explant cultures treated with iododeoxyuridine. the results suggest that epithelial cells of at least certain nasopharyngeal carcinomas possess the receptor for the epstein-barr virus and that ...1976176664
[new views in medical virology]. 1975176701
cell cycle dependence for activation of epstein-barr virus by inhibitors of protein synthesis or medium deficient in arginine. 1976176789
partial elimination of latent epstein-barr virus genomes from virus-producing cells by cyclohexamide. 1976176800
expression of epstein-barr-virus-associated membrane antigen in raji cells superinfected with two different virus strains. 1976176804
[infectious mononucleosis. 1. etiology, pathology, pathophysiology and epidemiology]. 1975176832
[application of a demographic method for the study of adenoviruses and herpesviruses in a closed infantile community (author's transl)].rate of seroconversions to adenoviruses, cytomegaloviruses (cmv) and epstein-barr virus (ebv) was studied from 1965-1973 in 200 infants living in a closed infantile community. the transversal demographic table method was used to evaluate the results of sera examined. seroconversion occurs earlier in the case of adenoviruses with also possess in higher intensity of infection. the intensity of seroconversions to ebv capsid antigen is a little lower, but of all three viruses examined, the spreading ...1975176843
infectious mononucleosis in a patient with hodgkin's disease.the case is presented of a patient, treated for hodgkin's disease, who contracted infectious mononucleosis more than 3 years later. while epstein-barr virus has been considered a possible etiologic factor in hodgkin's disease, the sequence of events reported in this case has to be interpreted as evidence against a causal relationship between the virus and hodgkin's disease in this patient.1976176858
positive mono-spot test in histiocytic medullary reticulosis.a case of rapidly fatal histiocytic medullary reticulosis in which two positive mono-spot tests were found is presented. in addition, there was a low liter antibody to eb virus. the patient died with a bleeding diathesis terminally. postmortem examinations confirmed the diagnosis of histiocytic medullary reticulosis.1976176885
characterization of epstein-barr virus (ebv) antigens ii. detection of early antigen (s) using anticomplement immunofluorescence (acif) et complement fixation (cf) tests.using human sera having antibodies against epstein-barr virus (ebv) early antigens (ea) but lacking antibodies directed against the eb nuclear antigen (ebna), the detection of ebv/ea was possible both by the anticomplement immunofluorescent test (acif) and by the classical complement fixation test (cf). the latter is particularly suitable for biochemical studies on ea.1975177116
letter: in vitro induction of ebv early antigens in npc epithelial tumour cells by iudr or superinfection.induction of early antigens in the epithelial tumour cells of npc was achieved both by iudr treatment and ebv superinfection.1975177117
[immunologic status in hodgkin patients: correlation with epstein-barr virus titers].1. in hodgkin's disease patient's immunological in vitro and in vivo parameters are of prognostic importance. 2. skin test reactivity correlates to peripheral t-lymphocyte counts and con a induced lymphoblastogenesis. 3. con a is the most sensitive in vitro indicator for detecting latent immunodeficiency. 4. hodgkin patients in long term remission after tumor reductive therapy exhibit a qualitative and quantitative lymphocyte defect. 5. in hodgkin patients herpes virus related antibody titers ar ...1976177135
primary intracranial burkitt's lymphoma in an infant.a case of intracranial burkitt's lymphoma is reported in a child whose symptoms began at 3 months of age with a definite histologic diagnosis established at 18 months. serologic studies demonstrated high antibody titers to epstein-barr virus (ebv) in the patient and in four out of five members of the immediate family. the patient also demonstrated immunity to antigens derived from african burkitt's lymphoma cell lines. the autopsy findings strongly support the case for the primary intracranial o ...1976177169
difference in antibody induced redistribution of membrane igm in ebv geonome free and ebv positive human lymphoid cells. 1976177301
epstein-barr virus interactions with human lymphocyte subpopulations: virus adsorption, kinetics of expression of epstein-barr virus-associated nuclear antigen, and lymphocyte order to further understand epstein-barr virus (ebv)-lymphocyte interactions, we investigated a chain of events including: (i) ebv binding to human lymphocyte subpopulations; (ii) the earliest appearance of ebv-determined nuclear antigen (ebna) in the lymphocytes after ebv infection; and (iii) establishment of continuous lymphoblastoid cell lines (lcl) by infecting with ebv different types of lymphocyte preparations from the same as well as from different donors. by using direct membrane immu ...1976177360
infectious mononucleosis. epstein-barr-virus shedding in saliva and the an examination of excretion patterns of epstein-barr virus in 104 throat washings from 20 patients with infectious mononucleosis we found that three persons regularly shed virus from the second week through the third month after onset; 15 demonstrated intermittent excretion over three months, and in two cases, no virus was detected. in oral secretions, the virus appeared to be located extracellularly. transforming activity was demonstrated in aliquots after centrifugation and filtration, in a ...1976177872
letter: epstein-barr viral antibodies and leukocyte-transforming agent in infectious mononucleosis. 1976177877
[association of nephrotic syndrome and hodgkin's disease. role of the epstein-barr virus].the clinical association between glomerulonephrtis (gn) and malignant hematological disease is very rare. we report, in a 24 years old male, the occurrence of an apparently idiopathic nephrotic syndrome with minimal change glomerular lesions. this gn was in fact closely related to the progression of a stage 2a of hodgkin's disease, following an infectious mononucleosis contracted one year ago. the nephrotic syndrome responded well to the therapy by prednisone and chlorambucil, and the complete r ...1976177950
epstein-barr virus (ebv)-induced change in the saturation sensitivity and serum dependence of established, ebv-negative lymphoma lines in vitro. 1976178101
smooth-muscle antibodies and antibodies to cytomegalovirus and epstein-barr virus in leukaemias and means of the indirect immunofluorescent method, smooth-muscle antibodies (sma) were detected significantly more often in patients with malignant diseases (11.7%) than in normal controls (3.3%) (0.02 greater than pgreater than 0.01). sma occurred in 23.8% of patients with hodgkin's disease, in 13.3% of patients with myeloproliferative diseases and in 4.7% of patients with lymphoproliferative diseases. other tissue antibodies were rare and they were found to occur at the same frequency in patie ...1976178146
infectious mononucleosis. 1976178158
facial diplegia.sudden facial diplegia or recurrent facial palsy is a rare occurrence, and may be the only symptom indicative of a systemic illness. two patients had this condition in the absence of guillain-barré-strohl syndrome.1976178296
[isolation of an epstein-barr type virus from cells of a suspended lymphoblastoid culture obtained from a patient with lymphogranulomatosis]. 1976178495
antinuclear antibodies and elevated anti-epstein-barr virus titers in cancer and one-half percent of human sera from patients seen at a clinic for treatment of cancer contained antibodies to the nuclei of chick kidney cells by indirect immunofluorescence tests. in the group of sera containing antinuclear antibodies, the geometric mean titer to epstein-barr virus (ebv) capsid antigen was significantly elevated. sera obtained from normal adults or from patients with similar histological types of tumors that possessed no antinuclear antibodies contained lower levels of ...1976178605
establishment and characterization of a human histiocytic lymphoma cell line (u-937).a human hematopoietic cell line (u-937) with exceptional characteristics was derived from a patient with generalized histiocytic lymphoma. the morphology of the cell line was identical to that of the tumor cells in the pleural effusion from which the line was derived. since epstein-barr virus (ebv) carrying diploid lymphoblastoid cell lines unrelated to the tumor population often become established in vitro from non-burkitt lymphoma explants, several parameters were studied to discriminate the u ...1976178611
activation in vitro by budr of a productive eb virus infection in the epithelial cells of nasopharyngeal carcinoma.material from a nasopharyngeal carcinoma (ncp) has been passaged in athymic (nude) mice to eliminate non-malignant infiltrating cells. the human origin and derivation from npc malignant epithelial cells of the nude mouse tumours have been confirmed by chromosome examination, electron microscopy showing desmosomes and keratin fibrils, and postive eb virus nuclear antigen (ebna) testing. samples of the mouse-grown tumours were cultured and pure monolayers of epithelial cells were obtained which st ...1976178612
antibodies to epstein-barr virus and cytomegalovirus in guillain-barre syndrome.twenty-three patients with guillain-barré syndrome (gbs) were investigated for serum antibody titres against epstein-barr virus capsid antigen (ebv-vca), cytomegalovirus (cmv) and herpes simplex virus type 1 (hsv1). the geometric mean ebv-vca titre was 45 in gbs, compared with 15-22 in healthy adults and 60 in sarcoidosis. nine of the 15 gbs patients (60%) from whom multiple serum specimens were available, displayed a four-fold variation in antibody titre to ebv-vca during the course of their di ...1976178829
covalently closed circular duplex dna of epstein-barr virus in a human lymphoid cell line. 1976178878
epstein-barr virus-specific rna. i. analysis of viral rna in cellular extracts and in the polyribosomal fraction of permissive and nonpermissive lymphoblastoid cell lines.we analyzed the viral rna in permissive and nonpermissive epstein-barr virus (ebv) pinfected lymphoblastoid cell lines by observing the kinetics of hybridization of labeled ebv hr-1 dna with unalabeled rna extracted from whole cells or from the polyribosomal fraction. the data indicate the following. (i) rna, homologous to only 3% of the ebv hr-1 dna, is present in the polyribosomal fraction of the nonpermissive namalwa and kurgans cells, suggesting that the function of only a small fraction of ...1976178893
nucleic acid renaturation and restriction endonuclease cleavage analyses show that the dnas of a transforming and a nontransforming strain of epstein-barr virus share approximately 90% of their nucleotide sequences.viral dna molecules were purified from a nontransforming and a transforming strain of epstein-barr virus. each viral dna was labeled in vitro and renatured in the presence of an excess of either one or the other unlabeled viral dna. both viral dnas were also digested with the eco r1 restriction endonuclease and subsequently labeled by using avian myeloblastosis virus dna polymerase to repair either the ecor1 nuclease-generated single-stranded ends of the dnas or their single-stranded ends produ ...1976178907
biological properties and viral surface antigens of burkitt lymphoma- and mononucleosis- derived strains of epstein-barr virus released from transformed marmoset cells.three strains of epstein-barr virus (ebv), two from burkitt lymphoma (bl) and one from infectious mononucleosis (im) were used to transform separate cultures of the same batch of primary marmoset leukocytes, and the viruses released from the transformants were compared. the three viruses shared properties of the transforming biotype of ebv, namely, stimulation of dna synthesis and immortalization of cord blood leukocytes, and failure to induce "early antigen" in lymphoblast lines. all viruses pr ...1976178913
[virus infections in man during immunodepression (author's transl)].the authors review virus infections observed in patients undergoing immunosuppressive treatment. the herpes virus, in particular the cytomegalovirus, are the most important cause. however, the role of the virus itself in an unfavourable course is variously assessed. the mechanism of the virus infection is discussed, whether primary infection, exogenous or endogenous re-infection. the latter seems to be the most frequent, at least in the case of the herpes viruses.1976179044
differential effect of phosphonoacetic acid on the expression of epstein-barr viral antigens and virus production.the effects of phosphonoacetic acid on cell growth, expression of epstein-barr virus antigens, and virus production in human and marmoset lymphoblastoid cell lines have been studied. the drug had no significant effect at concentrations up to 100 mug/ml on cell growth or total cell dna synthesis. higher doses induced not only a drastic decrease in dna synthesis and cell grwoth, but also a dramatic cell enlargement. immunofluorescence studies showed that greater than or equal to 30 mug/ml of phosp ...1976179098
diagnosis of infectious mononucleosis.infectious mononucleosis continues to be an important medical problem of young adults. diagnosis is based on three principal criteria: (1) a compatible clinical picture, (2) characteristic hematologic changes, and (3) a positive serology. a relative lymphocytosis of 60% or higher with at least 20% atypical lymphocytes is a common feature. laboratory diagnosis is dependent on proper application and interpretation of the various serologic tests. evidence of abnormal liver function is of particular ...1976179148
letter: epstein-barr virus and carcinoma. 1976179156
inhibition of productive replication of epstein-barr virus dna by phosphonoacetic acid. 1976179207
epstein-barr virus and nonhuman primates: natural and experimental infection. 1976179290
establishment and characterization of an epstein-barr virus (ebc)-negative lymphoblastoid b cell line (bja-b) from an exceptional, ebv-genome-negative african burkitt's epstein-barr virus (ebv)-negative lymphoblastoid cell line (lcl), bja-b, was established from an african burkitt's lymphoma (bl) which contained no detectable ebv dna and did not express the ebv specified antigen ebna, bja-b cells grow in typically large, flat clumps. all carry surface-bound immunoglobulins, a b lymphocyte marker, and do not form rosettes with sheep erythrocytes. after infection of bja-b cells by ebv the infected cells may produce either ebv-determined nuclear antigen (ebna) ...1975179629
diseases associated with herpesviruses.human herpesviruses have been associated with numerous diseases throughout history (table 3), but their ability to induce latent and recurrent infections, alongwith their oncogenic capabilities, is only beginning to be understood. accordingly, our ability to deal with the diseases induced by herpesviruses is severely limited by our lack of information concerning the basic processes of virus-host cell interactions and systemic host factors; especially immune factors that are involved in the disea ...1976179753
[atypical mononucleosis]. 1976179774
differential induction of epstein-barr virus-related antigens in human lymphoblastoid cells by virus-mediated cell-to-cell contact.differential induction of epstein-barr virus (ebv)-related antigens was observed after sendai virus-medicated fusion of producer and non-producer cells with various human and mouse cells. the ebv-determined early nuclear antigen (ena), early antigen (ea) and viral capsid antigen (vca) could be induced at a high rate in producer cells (p3hr-1 and b95-8), normally expressing these antigens at very low frequency, as early as 12-24 h after fusion with each other or with human amnion fl cells. in con ...1976179955
tumorigenicity of epstein-barr virus (ebv)-transformed lymphoid line cells in autologous squirrel monkeys.eight squirrel monkeys (saimiri sciureus) challenged with ebv or ebv-transformed slcl were naturally or experimentally infected with plasmodium knowlesi or pl. brasilianum. most of the animals had been splenectomized and unilaterally nephrectomized. three of these monkeys received one dose of 6 to 12 x 10(8) autologous slcl. these lines were derived from saimiri lymphoid cells permanently transformed by b-ebv in vitro. all three animals developed multiple undifferentiated malignant lymphomas and ...1976179960
appearance of epstein-barr virus-determined nuclear antigen in human epithelial cells following fusion with lymphoid cells.when human epithelial fl cells were fused with lymphoid cells harboring epstein-barr virus (ebv) genome, ebv-determined nuclear antigen (ebna) immunofluorescence became evident in high frequency in fl nuclei of heterokaryons several days after fusion. the implications of the findings are discussed in relation to the presence of ebv genome in nasopharyngeal carcinoma cells.1975179963
in vitro stimulation of lymphocytes of donors seronegative for epstein-barr virus by preparations of inactivated epstein-barr virus.heat-inactivated preparations of epstein-barr virus stimulated human lymphocytes as assayed by incorporation of [(3)h]thymidine. the inactivated epstein-barr virus stimulated the lymphocytes of all five seropositive donors, 11 of 14 seronegative donors (aged eight to 26 years), and none of 15 neonates. control antigens prepared from a human lymphoid cell line devoid of the epstein-barr virus genome did not stimulate the lymphocytes of seronegative donors. fetal calf serum at the concentration us ...1976180190
use of a transfection method to demonstrate a monolayer cell transforming agent from the eb3 line of burkitt's lymphoma cells.primary human amnion cell monolayers which had been treated with deae-dextran, washed, and then inoculated with sonicated cells of the eb3 line of burkitt's lymphoma cells developed foci of transformed amnion cells 7 to 14 days later. when either the deae-dextran or the sonicate was omitted, no significant transformation was found. the foci consisted of enlarging mounds of rapidly dividing cells, which upon subculturing continued their high miotic activity; and strains or lines of the transforme ...1976180248
antibodies to epstein-barr virus in neurological association of evidence of epstein-barr virus (ebv) infection in 3 different cases of neurological disease not related to infectious monomucleosis is reported. a rise in antibody titres to ebv-viral capsid antigen (vca) and early antigen (ea) in the serum and csf was demonstrated in a patient with acute encephalitis and in a patient with a psychotic-like organic brain syndrome. both patients demonstrated albuminocytological dissociation in the cerebrospinal fluid. igm antibodies specific to e ...1976180262
tonsillectomy and infectious mononucleosis--a possible relationship.a review of 1,192 student medical records shows a prior history of tonsillectomy in 41 percent of a control group and 22 percent of an infectious mononucleosis group--a significant difference (p less than 0.01). nine hundred eighty-nine stanford university students (421 females, 568 males) who visited cowell student health center between april and september, 1973, comprise the control group. two hundred three students with positive monospot tests and clinical mononucleosis diagnosed between june ...1976180364
[burkitt's lymphoma (author's transl)].burkitt's lymphoma occurs endemically in central africa, but is also found sporadically in areas with temperate climate. a case of burkitt's lymphoma found in our area is described. the literature in regard to the clinical symptoms, etiology and treatment of burkitt's lymphoma is reviewed. at the present time cyclophosphamide is considered to be the drug of choice; it leads to a remission rate of about 90%.1976180474
[immunologic study of suspension cultures prepared from hematopoietic organ cells of baboons with lymphoma].the cytoplasmic antigen was determined by the indirect immunofluorescence test using serum from a burkitt lymphoma patient with a titer of antibody to epstein-barr virus capsid antigen of 1.320, in the cytoplasm of 2--6% of cells of suspension cultures obtained from baboons with lymphoma. there was a correlation between the number of antigen-containing cells in this cultures and the number of cells in which herpes virus was determined by electron microscope studies. sera from baboons with lympho ...1976180690
production of infectious epstein-barr virus by using an intermittent culture collection system.procedures were developed to produce infectious epstein-barr virus in large glass fermentors with limited mechanical agitation by using an intermittent culture collection system. the system appears quite suitable for use in fermentation vessels of 50 liters or larger.1976180889
[prospective epidemiologic studies and the nature of the association between epstein-barr virus (ebv) and burkitt's lymphoma (bl)].the prospective study carried out by the international agency for research on cancer on the relationships between e.b.v. infection and burkitt's lymphoma development as well as their partial malaria chemoprophylaxy proposals, are discussed as an example of studying the role of a virus and of co-factors in one human cancer.1976181158
analysis of malignancy and antigen expression by cell fusion. 1976181274
surface markers on human b and t-lymphocytes. ix. two-color immunofluorescence studies on the association between ebv receptors and complement receptors on the surface of lymphoid cell lines.receptors for the third component of complement (c3) were demonstrated on the surface of established human lymphoid cell lines by a membrane fluorescence test with fitc- or tritc-conjugated antibodies against human c3. two-color fluorescence staining of ebv receptors and c3 receptors showed complete overlapping of green and red fluorescence. capping of the ebv receptor induced co-capping of the c3 receptor and vice versa. there was neither overlapping nor co-capping when ebv or c3 receptors were ...1976181330
detection of epstein-barr virus dna in american burkitt's lymphoma.twenty american patients with burkitt's lymphoma (bl) were studied serologically and biochemically to detect an association with epstein-barr virus (ebv). a wide range of antibody reactivities was encountered. five patients had serological profiles similar to those of african patients with bl: one and possibly two of these patients harbored ebv-dna in their tumors. fifteen patients had low or absent antibody to ebv: tumors from 10 of these patients lacked evidence of ebv-dna. an unexplained asso ...1976181331
epstein-barr virus: experimental infection of callithrix jacchus marmosets.eight common marmoset monkeys (callithrix jacchus) were inoculated with about 10(4) transforming units of b95-8 virus; seven of the marmosets died 50-111 days post inoculation and all seven showed microscopic and/or macroscopic lesions compatible with a diagnosis of lymphoproliferative disease. low levels of anti-vca antibodies were detected in plasma from six marmosets. attempts failed to establish continuous ebv-carrying lymphoblastoid cell cultures by cultivation in vitro of circulating lymph ...1976181333
anti-ebv antibody titers in non-hodgkin lymphomas.antibody titers to epstein-barr virus (ebv)-related antigens, i.e. viral capsid antigen (vca), the d and r components of the early antigen (ea) complex and the ebv-associated nuclear antigen (ebna), were determined in a series of 86 patients with non-hodgkin lymphomas and in 150 matched control subjects. the lymphoma patients belonged to four histological groups: diffuse, nodular, hyperbasophilic malignant lymphoma (hml) and unclassified. the ebv-related serological data were compared to the inc ...1976181335
production of lymphoid tumors in hamsters by direct implantation of normal human peripheral and umbilical cord leukocytes.peripheral leukocytes from epstein-barr virus (ebv)-seropositive or seronegative normal adults, or human umbilical cord leukocytes infected or not infected with ebv, were directly transplanted into 50 newborn hamsters treated with anti-lymphocyte serum. lymphoid tumors were produced after a latent period of 10-25 days in 39 animals: 8 of 14 recipients of seropositive donor leukocytes, 2 of 2 recipients of seronegative donor leukocytes, 13 of 15 recipients of ebv-infected cord leukocytes, and 16 ...1976181336
a quantitative analysis of the susceptibility of human leukocytes to transformation by epstein-barr virus.susceptibility of lymphocyte-enriched cell fractions isolated from human umbilical cord blood and adult peripheral blood to transformation by the b95-8 strain of epstein-barr virus (ebv) was investigated quantitatively. minimum multiplicity of input of virus (50% transforming dose) per cell (moi) necessary to induce maximum level transformation of cord cells ranged from 0.02 to 0.2. the frequency of initially transformed cells (fraction of transformable cells) in the cord cell samples from two d ...1976181337
an ebv-genome-negative cell line established from an american burkitt lymphoma; receptor characteristics. ebv infectibility and permanent conversion into ebv-positive sublines by in vitro in vitro line was derived from an american burkitt lymphoma, designated ra no. 1, which produced malignant tumors when inoculated into thymus-deficient nude mice. the cells have b-lymphocyte characteristics, with surface-associated mu and kappa chains and epstein-barr virus (ebv) receptors, and can be readily infected with ebv in vitro. b95-8 virus induced ebv-determined nuclear antigen (ebna) but not early antigen (ea) in ra no. 1 cells, whereas p3hr-1 virus induced both ebna and ea, but the ...1975181343
mechanisms of infection with epstein-barr virus. i. viral dna replication and formation of noninfectious virus particles in superinfected raji cells.human lymphoblastoid raji cells, which do not produce virus, supported replication of epstein-barr virus (ebv) upon superinfection. early antigen, viral capsid antigen, and virions were produced in raji cells superinfected with ebv. viral dna replicated under complete inhibition of host cell dna synthesis to the extent that a few micrograms of ebv dna were recovered from 107 superinfected raji cells, corresponding to 5,000 viral genomes/cell. homology of the synthesized viral dna to parental ebv ...1976181594
[human cancer and herpesviruses. eb virus. key points in carcinogenesis]. 1976181610
[human cancer and herpesviruses. eb virus. immunological studies of nasopharyngeal cancer]. 1976181611
[human cancer and herpesviruses. eb virus. carcinogenesis in vitro by eb virus]. 1976181612
[human cancer and herpesviruses. eb virus. presence of eb virus virus genes in tumor cells]. 1976181613
[immunological cell surface markers in infectious mononucleosis (author's transl)].using a direct immunofluorescence technique and a spontaneous rosette formation with sheep red blood cells it could be demonstrated that the large mononuclear cells in infectious mononucleosis consisted mainly of t-lymphocytes. it is likely that infectious mononucleosis represents a primary b-lymphcoyte infection with the epstein-barr virus like in the burkitt lymphoma. in contrast to this malignant lymphoma, interaction in infectious mononucleosis leads to a secondary t-lymphocyte proliferation ...1976181637
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