PMID(sorted ascending)
epstein-barr virus-positive large cell neuroendocrine carcinoma of the nasopharynx: report of a case with complete clinical and radiological response after combined chemoradiotherapy.neuroendocrine carcinomas of the head and neck are rare and are classified as well differentiated, moderately differentiated, and poorly differentiated carcinomas with the latter category being subdivided into small cell and large cell neuroendocrine carcinoma (lcnec). while most carcinomas in the nasopharynx are associated with epstein-barr virus (ebv), there has been only one previous report demonstrating a link between ebv and lcnec of the nasopharynx. in this report we describe a second case ...201829302900
new interactors of the truncated ebna-lp protein identified by mass spectrometry in p3hr1 burkitt's lymphoma cells.the epstein-barr virus nuclear antigen leader protein (ebna-lp) acts as a co-activator of ebna-2, a transcriptional activator essential for epstein-barr virus (ebv)-induced b-cell transformation. burkitt's lymphoma (bl) cells harboring a mutant ebv strain that lacks both the ebna-2 gene and 3' exons of ebna-lp express y1y2-truncated isoforms of ebna-lp (tebna-lp) and better resist apoptosis than if infected with the wild-type virus. in such bl cells, tebna-lp interacts with the protein phosphata ...201829303964
viruses in chronic progressive neurologic disease.viruses have long been implicated as triggers of disease onset and progression in multiple sclerosis (ms) and similar neuroinflammatory disorders. decades of epidemiological, molecular, and pathologic studies have most strongly linked the human herpesviruses epstein-barr virus (ebv) and human herpesvirus 6 (hhv-6) with ms. however, these viruses are ubiquitous in the general population and typically acquired decades before disease presentation, complicating the study of how they might contribute ...201829307291
the aliens inside us: herv-w endogenous retroviruses and multiple sclerosis.two human endogenous retroviruses of the herv-w family are proposed as multiple sclerosis (ms) co-factors: ms-associated retrovirus (msrv) and ervwe1, whose env proteins showed several potentially neuropathogenic features, in vitro and in animal models. phase ii clinical trials against herv-wenv are ongoing. herv-w/msrv was repeatedly found in ms patients, in striking parallel with ms stages, active/remission phases, and therapy outcome. the herv-wenv protein is highly expressed in active ms pla ...201829307292
are infectious agents involved in the pathogenesis of postpartum psychosis?since postpartum psychosis has been linked to activation of the immune system, it has been hypothesized that infectious agents may be involved in the pathogenesis of this disorder. we therefore investigated whether exposure to pathogens that can infect the central nervous system is increased in patients with postpartum psychosis.201829310062
signaling by the epstein-barr virus lmp1 protein induces potent cytotoxic cd4+ and cd8+ t cell responses.the b-lymphotropic epstein-barr virus (ebv), pandemic in humans, is rapidly controlled on initial infection by t cell surveillance; thereafter, the virus establishes a lifelong latent infection in the host. if surveillance fails, fatal lymphoproliferation and lymphomagenesis ensue. the initial t cell response consists of predominantly cd8+ cytotoxic t cells and a smaller expansion of cd4+ cells. a major approach to treating ebv-associated lymphomas is adoptive transfer of autologous or allogenei ...201829311309
epstein-barr virus, but not cytomegalovirus, latency accelerates the decay of childhood measles and rubella vaccine responses-a 10-year follow-up of a swedish birth cohort.epstein-barr virus (ebv) and cytomegalovirus (cmv) are ubiquitous and persistent herpesviruses commonly acquired during childhood. both viruses have a significant impact on the immune system, especially through mediating the establishment of cellular immunity, which keeps these viruses under control for life. far less is known about how these viruses influence b-cell responses.201729312344
novel x-linked inhibitor of apoptosis mutation in very early-onset inflammatory bowel disease child successfully treated with hla-haploidentical hemapoietic stem cells transplant after removal of αβ+ t and b cells.monogenic defects in genes related to primary immunodeficiencies can be responsible for inflammatory bowel disease (ibd). mutations in the x-linked inhibitor of apoptosis (xiap) gene have been described in several patients suffering from ibd and, in particular, with very early-onset inflammatory bowel disease (veoibd) features. we report a veoibd child with a novel xiap gene mutation characterized by a complicated disease course, which is unresponsive to several medical treatment options. a next ...201729312354
quantitative pcr assays of cytomegalovirus and epstein-barr virus in hemophagocytic lymphohistiocytosis. 201829313310
the potential of currently unavailable herpes virus vaccines.despite overwhelming experimental work, there are no licensed vaccines against the most frequent alphaherpesviruses, namely herpes simplex virus 1 and 2 (hsv1 and 2) nor against the epstein-barr virus (ebv), a member of the subfamily gammaherpesvirus. areas covered: since the dnas of both hsvs reside in the regional sensory ganglia in a latent state (i.e. as circularized episomal molecules), a corresponding vaccine might be useful for immunotherapy rather than prevention of primary infection. he ...201829313728
tumor-specific t-cells engineered to overcome tumor immune evasion induce clinical responses in patients with relapsed hodgkin lymphoma.purpose transforming growth factor-β (tgf-β) production in the tumor microenvironment is a potent and ubiquitous tumor immune evasion mechanism that inhibits the expansion and function of tumor-directed responses; therefore, we conducted a clinical study to discover the effects of the forced expression of a dominant-negative tgf-β receptor type 2 (dnrii) on the safety, survival, and activity of infused tumor-directed t cells. materials and methods in a dose escalation study, eight patients with ...201829315015
levels of oxidative stress biomarkers and bone resorption regulators in apical periodontitis lesions infected by epstein-barr investigate whether apical periodontitis lesions infected by epstein-barr virus (ebv) exhibit higher levels of oxidative stress biomarkers (8-hydroxydeoxyguanosine (8-ohdg) and oxidized glutathione (gssg)) and bone resorption regulators (receptor activator of nuclear factor (nf-κb) ligand (rankl) and osteoprotegerin (opg)) compared to ebv negative periapical lesions and healthy pulp tissues.201829315650
herpesviruses viral loads and levels of proinflammatory cytokines in apical periodontitis.this study aimed to analyse epstein-barr virus (ebv) and human cytomegalovirus (hcmv) viral loads in symptomatic and asymptomatic apical periodontitis lesions, to determine levels of tnf-α, il-1β, and il-6 in these lesions and to investigate a possible correlation between herpesviral copy numbers and levels of proinflammatory cytokines .201829316040
hodgkin's lymphoma and its association with ebv and hiv infection.hodgkin's lymphoma (hl) constitutes a clonal expansion of what appears to be malignant b cells. viruses are involved in its pathogenesis, such as the epstein-barr virus (ebv) and human immunodeficiency virus (hiv). since these viral infections have been shown to play key roles in the pathogenesis of hl, countries with a prevalence of hiv and ebv represent interesting population targets to study the pathogenesis of hl, linking the evolution of the disease with viral infections. usually, patients ...201829316828
epstein-barr virus-encoded rnas (ebers) complement the loss of herpesvirus telomerase rna (vtr) in virus-induced tumor formation.marek's disease virus (mdv) is an alphaherpesvirus that causes fatal lymphomas in chickens and is used as a natural virus-host model for herpesvirus-induced tumorigenesis. mdv encodes a telomerase rna subunit (vtr) that is crucial for efficient mdv-induced lymphoma formation; however, the mechanism is not completely understood. similarly, epstein barr-virus (ebv) encodes two rnas (eber-1 and eber-2) that are highly expressed in ebv-induced tumor cells, however their role in tumorigenesis remains ...201829317752
diagnostic value of the epstein-barr virus serological markers in patients with nasopharyngeal carcinoma in cases of undetectable primary tumor location.the goal of this work was to describe a method for diagnosis of the non-keratinizing nasopharyngeal carcinoma (nnpc) in cases of the undetectable primary tumor location. the method is based on evaluation of igg and iga antibody levels to the capsid (vca) and early antigens (ea) of the epstein-barr virus (ebv). the diagnosis of nnpc is established by a so-called decision rule. the latter was created by mathematical processing of the method of multifactor analysis of the results of anti-ebv antibo ...201829323852
epstein-barr virus (ebv)-induced b-cell lymphoproliferative disorder mimicking the recurrence of ebv-associated hemophagocytic lymphohistiocytosis.we describe the case of a 23-month-old male infant with epstein-barr virus (ebv)-associated lymphoproliferative disorder, which mimicked the recurrence of ebv-associated hemophagocytic lymphohistiocytosis. chemotherapy with dexamethasone, etoposide, and cyclosporine resolved fever, hepatosplenomegaly, and pancytopenia. however, on day 81 of illness, the patient developed similar symptoms. plasma ebv-dna levels markedly increased again, but no t-cell clonality was observed. b cells were identifie ...201829324572
[opportunistic infection in systemic lupus erythematosus patients: the disease spectrum and the characteristics of peripheral lymphocyte subsets].objective: to investigate the common opportunistic infections and the characteristics of peripheral lymphocyte subsets in patients with systemic lupus erythematosus (sle). methods: from december 2013 to december 2016, peripheral lymphocyte subsets were consecutively detected by flow cytometry in treated sle patients with or without opportunistic infections (ois) . the lymphocyte subsets in healthy donors were used as normal control group. results: a total of 145 treated sle patients were enrolle ...201829325308
considerations in the diagnosis of oral hairy leukoplakia-an institutional experience.we report here the 10-year experience with oral hairy leukoplakia (ohl) at the division of oral and maxillofacial pathology at the university of north carolina at chapel hill, nc, usa.201729325854
dna methylation enzyme inhibitor rg108 suppresses the radioresistance of esophageal cancer.esophageal cancer (ec) is the eighth most common highly aggressive cancer worldwide. the purpose of this study was to investigate the effect of the dna methyltransferase inhibitor rg108 on the radiosensitivity of ec cells. mtt and clonogenic assays were performed to assess the effect of rg108 on the proliferation and radiosensitivity of eca‑109 and te‑1 human ec cells. the cell cycle progression and alterations in apoptosis were analyzed by flow cytometry. for the in vivo analysis, the eca‑109 c ...201829328411
the interactome of ebv lmp1 evaluated by proximity-based bioid approach.epstein-barr virus lmp1 is an oncoprotein required for immortalizing b lymphocytes and also plays important roles in transforming non-lymphoid tissue. the discovery of lmp1 protein interactions will likely generate targets to treat ebv-associated cancers. here, we define the broader lmp1 interactome using the recently developed bioid method. combined with mass spectrometry, we identified over 1000 proteins across seven independent experiments with direct or indirect relationships to lmp1. pathwa ...201829329079
mutations in pi3k110δ cause impaired nk cell function partially rescued by rapamycin treatment.heterozygous gain-of-function mutations in pi3k110 lead to lymphadenopathy, lymphoid hyperplasia, epstein-barr virus (ebv) and cytomegalovirus (cmv) viremia, and sinopulmonary infections.201829330011
human papilloma virus-specific t cells can be generated from naïve t cells for use as an immunotherapeutic strategy for immunocompromised patients.human papilloma virus (hpv) is a known cause of cervical cancer, squamous cell carcinoma and laryngeal cancer. although treatments exist for hpv-associated malignancies, patients unresponsive to these therapies have a poor prognosis. recent findings from vaccine studies suggest that t-cell immunity is essential for disease control. because epstein-barr virus (ebv)-specific t cells have been highly successful in treating or preventing ebv-associated tumors, we hypothesized that the development of ...201829331266
the predictive value of pre- and post-induction chemotherapy plasma ebv dna level and tumor volume for the radiosensitivity of locally advanced nasopharyngeal carcinoma.this study was dedicated to investigate the predictive value of pre- and post-induction chemotherapy plasma ebv (epstein-barr virus) dna level and tumor volume for the radiosensitivity of locally advanced npc. 129 previously untreated locally advanced npc patients were enrolled. plasma ebv-dna copy number and tumor volume was detected before and after induction chemotherapy. the tumor volume was also measured after radiotherapy. among 129 patients, 98 were positive for ebv dna. the residual gros ...201729333129
epstein barr virus and invasive mammary carcinomas: ebna, ebers and molecular profile in a population of west algeria.breast cancer is the common malignancy that affects women worldwide, but conventional risk factors account for only a small proportion of these cases. a possible viral etiology for breast cancer has been proposed and epstein-barr virus (ebv) is a widely studied candidate virus. the objective of this study is to determine the association of ebv infection with infiltrating ductal carcinomas (idc). this descriptive study was carried out in the laboratory of developmental biology and differentiation ...201829336321
modulating gene expression in epstein-barr virus (ebv)-positive b cell lines with crispra and crispri.epstein-barr virus (ebv) transforms small resting primary b cells into large lymphoblastoid cells which are able to grow and survive in vitro indefinitely. these cells represent a model for oncogenesis. in this unit, variants of conventional clustered regularly interspaced short palindromic repeats (crispr), namely the crispr activation (crispra) and crispr interference (crispri) methods, are discussed in the context of gene regulation at genomic dna promoter and enhancer elements. lymphoblastoi ...201829337370
crispr/cas9-mediated genome editing in epstein-barr virus-transformed lymphoblastoid b-cell lines.epstein-barr virus (ebv) efficiently transforms primary human b cells into immortalized lymphoblastoid cell lines (lcls), which are extensively used in human genetic, immunological and virological studies. lcls provide unlimited sources of dna for genetic investigation, but can be difficult to manipulate, for instance because low retroviral or lentiviral transduction frequencies hinder experiments that require co-expression of multiple components. this unit details clustered regularly interspace ...201829337376
epstein-barr virus infection and type i interferon signature in patients with systemic lupus erythematosus.epstein-barr (eb) virus infection has long been speculated to evoke systemic lupus erythematosus (sle). since a virus infection can induce interferon (ifn) system activation, we aimed to discover the relationship between the two in the progression of sle in a chinese inpatient cohort. methods peripheral blood mononuclear cells and sera were isolated from 116 sle patients and 76 healthy controls. antibodies against ebv-vca (igm and igg) and ebna (igg) along with ifnα in patient sera were detected ...201829338588
transcriptional profile and epstein-barr virus infection status of laser-cut immune infiltrates from the brain of patients with progressive multiple is debated whether multiple sclerosis (ms) might result from an immunopathological response toward an active epstein-barr virus (ebv) infection brought into the central nervous system (cns) by immigrating b cells. based on this model, a relationship should exist between the local immune milieu and ebv infection status in the ms brain. to test this hypothesis, we analyzed expression of viral and cellular genes in brain-infiltrating immune cells.201829338732
what is the impact of epstein-barr virus in peri-implant infection?to compare the qualitative and quantitative profile of epstein-barr virus (ebv) at external and internal implant surfaces between participants with peri-implantitis and healthy peri-implant tissues and to quantitatively assess the relation between ebv and periopathogens inside the microbiologic profile associated with peri-implantitis.201829340343
incidence and predictors of post-transplant lymphoproliferative disease after kidney transplantation during adulthood and childhood: a registry study.differences in the epidemiology of post-transplant lymphoproliferative disease (ptld) between adult and paediatric kidney transplant recipients remain unclear.201829342279
[x-linked immunodeficiency with magnesium defect, epstein-barr virus infection, and neoplasia: report of a family and literature review].objective: to investigate the clinical features and genetic characteristics of cases with x-linked immunodeficiency with magnesium defect, epstein-barr virus (ebv) infection, and neoplasia (xmen). methods: characteristics of clinical material, immunological data and gene mutation of two cases with xmen in the same family in china were retrospectively analyzed. the related reports literature were searched by using search terms'magt1 gene'or'xmen'. results: the proband, a 2-year-eight-month old bo ...201829342998
gamma-herpesvirus colonization of the spleen requires lytic replication in b cells.gamma-herpesviruses infect lymphocytes and cause lymphocytic cancers. murid herpesvirus-4 (muhv-4), epstein-barr virus and the kaposi's sarcoma-associated herpesvirus all infect b cells. latent infection can spread by b cell recirculation and proliferation, but whether this alone achieves systemic infection is unclear. to test the need of muhv-4 for lytic infection in b cells we flanked its essential orf50 lytic transactivator with loxp sites, then infected mice with b cell-specific cre expressi ...201829343572
orbital epstein-barr virus-positive polymorphic b-cell lymphoproliferative disorder in an apparently immunocompetent woman.we report a rare case of epstein-barr virus (ebv)-positive polymorphic b-cell lymphoproliferative disorder (lpd) involving the lacrimal gland of a 28-year-old, apparently immunocompetent woman. she presented with a chief complaint of orbital swelling and tenderness and was found to have a lesion involving the right lacrimal gland and distal superior and lateral rectus muscles. histology of the lesion revealed histiocytes with pleomorphic nuclei, reactive lymphocytes, and scattered cells that res ...201729344502
therapeutic manipulation of host cell death pathways to facilitate clearance of persistent viral infections.most persistent viral infections can be controlled, but not cured, by current therapies. abrogated antiviral immunity and stable latently infected cells represent major barriers to cure. this necessitates life-long suppressive antiviral therapy. achieving a cure for hiv, hepatitis b virus, epstein barr-virus, and others, requires novel approaches to facilitate the clearance of infected cells from the host. one such approach is to target host cell death pathways, rather than the virus itself. her ...201829345371
microsatellite stability and mismatch repair proficiency in nasopharyngeal carcinoma may not predict programmed death-1 blockade resistance.the us fda granted accelerated approval to pembrolizumab for microsatellite instability-high and mismatch repair deficient cancers. the response of programmed death-1 blockade in mismatch repair proficiency (pmmr) colorectal cancer is very poor, however, whether such treatment is effective in pmmr nasopharyngeal carcinoma (npc) remains unknown. we report a case of a 51-year-old man with npc. pet-ct scan revealed a space-occupying lesion in the left lung, and the pathologic result confirmed the o ...201729348907
elevated levels of interferon-γ are associated with high levels of epstein-barr virus reactivation in patients with the intestinal type of gastric cancer.the inflammatory response directed against helicobacter pylori (hp) is believed to be one of the main triggers of the appearance of gastric lesions and their progression to gastric cancer (gc). epstein-barr virus (ebv) has been found responsible for about 10% of all gcs, but the inflammatory response has not been studied in gc patients with evidence of high levels of ebv reactivation.201729349089
membranoproliferative glomerulonephritis and interstitial nephritis in the setting of epstein-barr virus-related hemophagocytic syndrome
.hemophagocytic syndrome (hps) is a rare, aggressive disorder characterized by dysregulation of lymphocyte and macrophage activity, which culminates in tissue infiltration with hemophagocytosis and ultimately organ failure. renal involvement frequently ensues and usually results in acute tubular necrosis with associated interstitial inflammation. less frequently, glomerulopathy can also be found.201829350173
epstein-barr virus encoded latent membrane protein 1 suppresses necroptosis through targeting ripk1/3 ubiquitination.necroptosis is an alternative programmed cell death pathway that is unleashed in the absence of apoptosis and mediated by signaling complexes containing receptor-interating protein kinase 1 (ripk1) and ripk3. this form of cell death has recently been implicated in host defense system to eliminate pathogen-infected cells. however, only a few viral species such as herpes simplex virus (hsv) and cytomegalovirus (cmv) have evolved mechanisms inhibiting necroptosis to overcome host antiviral defense, ...201829352166
current perspectives on erythema multiforme.recognition and timely adequate treatment of erythema multiforme remain a major challenge. in this review, current diagnostic guidelines, potential pitfalls, and modern/novel treatment options are summarized with the aim to help clinicians with diagnostic and therapeutic decision-making. the diagnosis of erythema multiforme, that has an acute, self-limiting course, is based on its typical clinical picture of targetoid erythematous lesions with predominant acral localization as well as histologic ...201829352387
characterization of skin blister fluids from children with epstein-barr virus-associated lymphoproliferative disease.epstein-barr virus (ebv)-associated t- or natural killer (nk)-cell lymphoproliferative disease (lpd) is a heterogeneous group of disorders characterized by chronic proliferation of ebv-infected lymphocytes. patients may present with severe skin manifestations, including hypersensitivity to mosquito bites (hmb) and hydroa vacciniforme (hv)-like eruption, which are characterized by blister formation and necrotic ulceration. skin biopsy specimens show inflammatory reactions comprising ebv-infected ...201829352500
interferon regulatory factor 5 is a potential target of autoimmune response triggered by epstein-barr virus and mycobacterium avium subsp. paratuberculosis in rheumatoid arthritis: investigating a mechanism of molecular mimicry.rheumatoid arthritis (ra) is a chronic disease characterised by a pro-inflammatory cytokines linked erosive joint damage and by humoral and cellular response against a broad range of self-peptides. molecular mimicry between epstein-barr virus (ebv), mycobacterium avium subsp. paratuberculosis (map) and host peptides has long been regarded as an ra pathogenetic mechanism. using bioinformatic analysis we identified high sequence homology among interferon regulatory factor 5 (irf5), ebv antigen bol ...201829352853
association of cytomegalovirus and epstein-barr virus with cognitive functioning and risk of dementia in the general population: 11-year follow-up study.earlier studies have documented an association between cytomegalovirus and cognitive impairment, but results have been inconsistent. few studies have investigated the association of cytomegalovirus and epstein-barr virus with cognitive decline longitudinally. our aim was to examine whether cytomegalovirus and epstein-barr virus are associated with cognitive decline in adults method: the study sample is from the finnish health 2000 survey (brif8901, n = 7112), which is representative of the finni ...201829355820
interferon regulatory factor 8 regulates aspase-1 expression to facilitate epstein-barr virus reactivation in response to b cell receptor stimulation and chemical induction.interferon regulatory factor 8 (irf8), also known as interferon consensus sequence-binding protein (icsbp), is a transcription factor of the irf family. irf8 plays a key role in normal b cell differentiation, a cellular process that is intrinsically associated with epstein-barr virus (ebv) reactivation. however, whether irf8 regulates ebv lytic replication remains unknown. in this study, we utilized a crispr/cas9 genomic editing approach to deplete irf8 and found that irf8 depletion dramatically ...201829357389
herpesvirus deconjugases inhibit the ifn response by promoting trim25 autoubiquitination and functional inactivation of the rig-i signalosome.the n-terminal domains of the herpesvirus large tegument proteins encode a conserved cysteine protease with ubiquitin- and nedd8-specific deconjugase activity. the proteins are expressed during the productive virus cycle and are incorporated into infectious virus particles, being delivered to the target cells upon primary infection. members of this viral enzyme family were shown to regulate different aspects of the virus life cycle and the innate anti-viral response. however, only few substrates ...201829357390
epstein-barr virus and hemophagocytic lymphohistiocytosis.epstein-barr virus (ebv) is a ubiquitous virus that infects nearly all people worldwide without serious sequela. however, for patients who have genetic diseases which predispose them to the development of hemophagocytic lymphohistiocytosis (hlh), ebv infection is a life-threatening problem. as a part of a themed collection of articles on ebv infection and human primary immune deficiencies, we will review key concepts related to the understanding and treatment of hlh.201729358936
long-term follow up of post renal transplantation epstein-barr virus-associated smooth muscle tumors: report of two cases and review of the literature.epstein-barr virus (ebv)-associated smooth muscle tumors (smts) following solid organ transplantation are very rare slow growing neoplasms. most tumors present with non-specific symptoms mainly related to tumor location. post-transplant ebv-associated small muscle tumors have been reported in various anatomical locations. the tumors have a predilection to unusual sites for smts and tend to be multifocal. the histologic appearance of these tumors generally does not predict their clinical behavior ...201829359839
a novel epstein-barr virus-latent membrane protein-1-specific t-cell receptor for tcr gene therapy.adoptive transfer of genetically engineered t-cells to express antigen-specific t-cell receptor (tcr) is a feasible and effective therapeutic approach for numerous types of cancers, including epstein-barr virus (ebv)-associated malignancies. here, we describe a tcr gene transfer regimen to rapidly and reliably generate t-cells specific to ebv-encoded latent membrane protein-1 (lmp1), which is a potential target for t-cell-based immunotherapy.201829360818
[a case of cervical lymph node metastasis from an unknown primary cancer controlled with immunotherapy, chemotherapy, and surgery].we present a case of cervical lymph node metastasis from an unknown primary cancer that was controlled with immunotherapy, chemotherapy, and surgery. the patient, a 61-year-old man, was referred to our department for treatment of a lesion in the left cervical lateral area. at the initial visit, the mass was covered by reddened skin and was elastic, hard, and immobile on palpation. the presence of a malignant disease such as malignant lymphoma or lymphadenitis because of infection by tubercle bac ...201729361629
human mhc-ii with shared epitope motifs are optimal epstein-barr virus glycoprotein 42 ligands-relation to rheumatoid arthritis.rheumatoid arthritis (ra) is a chronic systemic autoimmune disorder of unknown etiology, which is characterized by inflammation in the synovium and joint damage. although the pathogenesis of ra remains to be determined, a combination of environmental (e.g., viral infections) and genetic factors influence disease onset. especially genetic factors play a vital role in the onset of disease, as the heritability of ra is 50-60%, with the human leukocyte antigen (hla) alleles accounting for at least 3 ...201829361739
mutation analysis of the ebv-lymphoblastoid cell line cautions their use as antigen-presenting cells.lymphoblastoid cell lines (lcls) have been widely used as professional antigen-presenting cells (apcs). however, neoantigen-loaded lcls could induce nonspecific t-cell response, which could be due to expression of both epstein-barr virus (ebv) antigens and nonsynonymous mutations arising in lcls. since the number of passages could influence mutational characteristics of lcls, and moreover extensive proliferation of lcls in vitro is necessary to activate t cells for immunotherapy, we comprehensiv ...201729363169
expression of activating nk-cell receptors is a hallmark of the innate-like t-cell neoplasm in peripheral t-cell lymphomas.peripheral t- or nk-cell lymphomas are rare and difficult-to-recognize diseases. it remains arduous to distinguish between nk cell- and cytotoxic t lymphocyte-derived lymphomas through routine histologic evaluation. to clarify the cells of origin, we focused on nk-cell receptors and examined the expression using immunohistochemistry in 22 cases with t- and nk-cell neoplasms comprising of angioimmunoblastic t-cell lymphoma, alk-positive and -negative anaplastic large cell lymphomas, extranodal nk ...201829363227
no increased risk of posttransplant lymphoproliferative disorder following alemtuzumab induction in kidney transplant.posttransplant lymphoproliferative disorder is a known complication of solid-organ transplant. the use of depleting induction agents has demonstrated varying associations with incidence of posttransplant lymphoproliferative disorder. alemtuzumab, a depleting induction agent for kidney transplant patients, has shown promising results in reducing the risk of acute rejection and graft loss in the first year. its unique mechanism of depleting both t-cell and b-cell populations may be beneficial in p ...201829363415
toll-like receptors 7 and 9 in myasthenia gravis thymus: amplifiers of autoimmunity?pathogen infections and dysregulated toll-like receptor (tlr)-mediated innate immune responses are suspected to play key roles in autoimmunity. among tlrs, tlr7 and tlr9 have been implicated in several autoimmune conditions, mainly because of their ability to promote abnormal b cell activation and survival. recently, we provided evidence of epstein-barr virus (ebv) persistence and reactivation in the thymus of myasthenia gravis (mg) patients, suggesting an involvement of ebv in the intrathymic p ...201829363775
[infectious mononucleosis and cholestatic hepatitis: a rare association].infectious mononucleosis is one of the major clinical manifestations of epstein-barr virus infection. in this syndrome, elevation of liver transaminase levels is common but cholestasis is rare, with few cases described in the literature. we present the case of a 14-year-old female adolescent, admitted to the emergency room with fever, odynophagia and cervical adenomegaly. she was treated with amoxicillin and two days later he presented with jaundice. the analytical evaluation was compatible with ...201729364802
a conserved eph family receptor-binding motif on the gh/gl complex of kaposi's sarcoma-associated herpesvirus and rhesus monkey rhadinovirus.kaposi's sarcoma-associated herpesvirus (kshv) is a human oncogenic virus associated with kaposi's sarcoma and two b-cell malignancies. the rhesus monkey rhadinovirus (rrv) is a virus of nonhuman primates that is closely related to kshv. eph family receptor tyrosine kinases (ephs) are cellular receptors for the gh/gl glycoprotein complexes of both kshv and rrv. through sequence analysis and mutational screens, we identified conserved residues in the n-terminal domain of kshv and rrv glycoprotein ...201829432452
co-amplification of ebna-1 and pylt through dhfr-mediated gene amplification for improving foreign protein production in transient gene expression in cho cells.despite the relatively low transfection efficiency and low specific foreign protein productivity (qp) of chinese hamster ovary (cho) cell-based transient gene expression (tge) systems, tge-based recombinant protein production technology predominantly employs cho cells for pre-clinical research and development purposes. to improve tge in cho cells, epstein-barr virus nuclear antigen-1 (ebna-1)/polyoma virus large t antigen (pylt)-co-amplified recombinant cho (rcho) cells stably expressing ebna-1 ...201829654557
optimization of a high-cell-density polyethylenimine transfection method for rapid protein production in cho-ebna1 cells.for pre-clinical evaluation of biotherapeutic candidates, protein production by transient gene expression (tge) in chinese hamster ovary (cho) cells offers important advantages, including the capability of rapidly and cost-effectively generating recombinant proteins that are highly similar to those produced in stable cho clones. we have established a novel cho clone (cho-3e7) expressing a form of the epstein-barr virus nuclear antigen-1 (ebna-1) with improved tge productivity relative to parenta ...201829886030
macaca arctoides gammaherpesvirus 1 (strain herpesvirus macaca arctoides): virus sequence, phylogeny and characterisation of virus-transformed macaque and rabbit cell lines.herpesvirus macaca arctoides (hvma) has the propensity to transform macaque lymphocytes to lymphoblastoid cells (mal-1). inoculation of rabbits with cell-free virus-containing supernatant resulted in the development of malignant lymphomas and allowed isolation of immortalised hvma-transformed rabbit lymphocytes (htrl). in this study, the hvma genome sequence (approx. 167 kbp), its organisation, and novel aspects of virus latency are presented. ninety-one open reading frames were identified, of w ...201930291474
experimental co-transmission of simian immunodeficiency virus (siv) and the macaque homologs of the kaposi sarcoma-associated herpesvirus (kshv) and epstein-barr virus (ebv).macaque rfhv and lcv are close homologs of human kshv and ebv, respectively. no experimental model of rfhv has been developed due to the lack of a source of culturable infectious virus. screening of macaques at the washington national primate research center detected rfhv in saliva of siv-infected macaques from previous vaccine studies. a pilot experimental infection of two naïve juvenile pig-tailed macaques was initiated by inoculation of saliva from siv-infected pig-tailed and cynomolgus macaq ...201830444879
comparative analysis of gammaherpesvirus circular rna repertoires: conserved and unique viral circular rnas.recent studies have identified circular rnas (circrnas) expressed from the epstein-barr virus (ebv) and kaposi's sarcoma herpesvirus (kshv) human dna tumor viruses. to gain initial insights into the potential relevance of ebv circrnas in virus biology and disease, we assessed the circrnaome of the interspecies homologue rhesus macaque lymphocryptovirus (rlcv) in a naturally occurring lymphoma from a simian immunodeficiency virus (siv)-infected rhesus macaque. this analysis revealed rlcv ortholog ...201930567979
herpes virus reactivation in astronauts during spaceflight and its application on earth.latent herpes virus reactivation has been demonstrated in astronauts during shuttle (10-16 days) and international space station (≥180 days) flights. following reactivation, viruses are shed in the body fluids of astronauts. typically, shedding of viral dna is asymptomatic in astronauts regardless of mission duration; however, in some cases, live/infectious virus was recovered by tissue culture that was associated with atopic-dermatitis or skin lesions during and after spaceflight. hypothalamic- ...201930792698
gammaherpesvirus rnas come full circle.after an adaptive immune response is mounted, gammaherpesviruses achieve persistence through the utilization of viral noncoding rnas to craft a suitable host cell environment in an immunologically transparent manner. while gammaherpesvirus long noncoding rnas (lncrnas) and micrornas have been recognized for some time and have been actively investigated, a recent spate of reports have now identified repertoires of the circular rna (circrna) class of noncoding rnas in both the lymphocryptovirus an ...201930940699
t-cell/histiocyte-rich large b-cell lymphoma of the larynx in a juvenile japanese macaque (macaca fuscata).an 11-month-old female japanese macaque (macaca fuscata), born in captivity in a research institute, suddenly died without clinical signs. necropsy examination revealed a nodular mass protruding from the left ventral aspect of the larynx, compressing the epiglottis anteriorly. histopathologically, the laryngeal mass was comprised of medium- to large-sized atypical cells. immunohistochemically, these were positive for cd20 and partially positive for cd79α. among the atypical cells were cd3+ t cel ...201931159945
a virome sequencing approach to feline oral squamous cell carcinoma to evaluate viral causative factors.feline oral squamous cell carcinoma (foscc) may be the best naturally-occurring model of human head and neck squamous cell carcinoma (hnscc). hnscc can be broadly divided into human papillomavirus (hpv)-negative cancers and hpv-positive cancers where hpv is the causative agent. previous studies in foscc have used both species-specific and species-nonspecific pcr primers that may be insensitive to the detection of pvs and other viruses that may be divergent from known sequences. virocap is a targ ...202031902496
viral opportunistic infections in mauritian cynomolgus macaques undergoing allogeneic stem cell transplantation mirror human transplant infectious disease complications.allogeneic hematopoietic stem cell transplantation (hsct) and xenotransplantation are accompanied by viral reactivations and virus-associated complications resulting from immune deficiency. here, in a mauritian cynomolgus macaque model of fully mhc-matched allogeneic hsct, we report reactivations of cynomolgus polyomavirus, lymphocryptovirus, and cytomegalovirus, macaque viruses analogous to hsct-associated human counterparts bk virus, epstein-barr virus, and human cytomegalovirus. viral replica ...202031930750
alcelaphine herpesvirus 1 genes a7 and a8 regulate viral spread and are essential for malignant catarrhal fever.alcelaphine herpesvirus 1 (alhv-1) is a gammaherpesvirus that is carried asymptomatically by wildebeest. upon cross-species transmission to other ruminants, including domestic cattle, alhv-1 induces malignant catarrhal fever (mcf), which is a fatal lymphoproliferative disease resulting from proliferation and uncontrolled activation of latently infected cd8+ t cells. two laboratory strains of alhv-1 are used commonly in research: c500, which is pathogenic, and wc11, which has been attenuated by l ...202032176737
development of an improved epstein-barr virus (ebv) neutralizing antibody assay to facilitate development of a prophylactic gp350-subunit ebv licensed vaccine is available for prevention of ebv-associated diseases, and robust, high-throughput bioanalytical assays are needed to evaluate immunogenicity of gp350 subunit-based candidate ebv vaccines. here we have developed an improved ebv-gfp based neutralization assay for such a vaccine's pre-clinical and clinical validation to measure ebv specific neutralizing antibodies in human donors. the supplementation of guinea pig complement of our previously published high-throughput ebv-gfp ...202032180911
post-translational protein deimination signatures in serum and serum-extracellular vesicles of bos taurus reveal immune, anti-pathogenic, anti-viral, metabolic and cancer-related pathways for deimination.the bovine immune system is known for its unusual traits relating to immunoglobulin and antiviral responses. peptidylarginine deiminases (pads) are phylogenetically conserved enzymes that cause post-translational deimination, contributing to protein moonlighting in health and disease. pads also regulate extracellular vesicle (ev) release, forming a critical part of cellular communication. as pad-mediated mechanisms in bovine immunology and physiology remain to be investigated, this study profile ...202032325910
recombinant epstein-barr virus glycoprotein 350 as a serological antigen.epstein-barr virus (ebv) glycoprotein 350 (gp350) is the most abundant glycoprotein expressed on the ebv envelope, the major target for neutralizing antibodies and also essential for virion attachment to b lymphocytes. several studies have addressed ebv gp350 as a vaccine candidate, but less commonly as a potential antigen for serological assays. the aim of the current study was to develop a diagnostic tool to quantify ebv gp350-specific igg in previously ebv-infected individuals. a construct en ...202032650039
neutralizing antibodies protect against oral transmission of lymphocryptovirus.epstein-barr virus (ebv) is a cancer-associated pathogen for which there is no vaccine. successful anti-viral vaccines elicit antibodies that neutralize infectivity; however, it is unknown whether neutralizing antibodies prevent ebv acquisition. here we assessed whether passively delivered ammo1, a monoclonal antibody that neutralizes ebv in a cell-type-independent manner, could protect against experimental ebv challenge in two animal infection models. when present prior to a high-dose intraveno ...202032724901
north american big brown bats (eptesicus fuscus) harbor an exogenous deltaretrovirus.bats are the reservoir for a large number of zoonotic viruses, including members of coronaviridae (severe acute respiratory syndrome coronavirus [sars-cov] and sars-cov-2), paramyxoviridae (hendra and nipah viruses), rhabdoviridae (rabies virus), and filoviridae (ebola virus) as exemplars. many retroviruses, such as human immunodeficiency virus, are similarly zoonotic; however, only infectious exogenous gammaretroviruses have recently been identified in bats. here, viral metagenomic sequencing o ...202032968009
antibody-mediated immune subset depletion modulates the immune response in a rabbit (oryctolagus cuniculus) model of epstein-barr virus infection.epstein-barr virus (ebv) is a γ-herpesvirus which infects over 90% of the adult human population. most notably, this virus causes infectious mononucleosis but it is also associated with cancers such as hodgkin and burkitt lymphoma. ebv is a species-specific virus and has been studied in many animal models, including nonhuman primates, guinea pigs, humanized mice, and tree shrews. however, none of these animal models are considered the "gold standard" for ebv research. recently, rabbits have emer ...202032972486
marek's disease virus meq oncoprotein interacts with chicken hdac 1 and 2 and mediates their degradation via proteasome dependent pathway.marek's disease virus (mdv) encodes a basic-leucine zipper (bzip) protein, meq, which is considered the major mdv oncoprotein. it has been reported that the oncogenicity of meq is associated with its interaction with c-terminal binding protein 1 (ctbp), which is also an interaction partner of epstein-barr virus encoded ebna3a and ebna3c oncoproteins. since both ebna3c and ctbp interact with histone deacetylase 1 (hdac1) and hdac2, we examined whether meq shares this interaction with chicken hdac ...202133437016
plxdc family members are novel receptors for the rhesus monkey rhadinovirus (rrv).the rhesus monkey rhadinovirus (rrv), a γ2-herpesvirus of rhesus macaques, shares many biological features with the human pathogenic kaposi's sarcoma-associated herpesvirus (kshv). both viruses, as well as the more distantly related epstein-barr virus, engage cellular receptors from the eph family of receptor tyrosine kinases (ephs). however, the importance of the eph interaction for rrv entry varies between cell types suggesting the existence of eph-independent entry pathways. we therefore aime ...202133657166
effect of epstein-barr virus dna on the incidence and severity of arthritis in a rheumatoid arthritis mouse model.we recently demonstrated that ebv dna is correlated with proinflammatory responses in mice and in rheumatoid arthritis (ra) patients; hence, we utilized an ra mouse model to examine whether ebv dna enhances the risk and severity of arthritis and to assess its immunomodulatory effects.202134040613
establishing an induced pluripotent stem cell line from neonatal common marmoset fibroblasts by an all-in-one episomal vector approach.epstein-barr virus (ebv)-based episomal vector system enables persistent transgene expression, which is advantageous for efficient derivation of transgene-free induced pluripotent stem cells (ipscs) without viral transduction. here, we report establishment of an ipsc line from somatic fibroblasts of a neonatal common marmoset monkey (marmoset; callithrix jacchus) using an all-in-one episomal vector that we newly developed. the established ipsc line, named nm-ips, showed standard characteristics ...202134088009
catching viral breast cancer.we have considered viruses and their contribution to breast cancer.202134108009
novel gammaherpesvirus associated with primary gastric t-cell lymphoma in a free-ranging giant armadillo in brazil.the number of viral-associated neoplasms reported in wildlife has increased over the last decades, likely because of growing research efforts and a potentially greater burden of carcinogenic pathogens. herein, we describe a primary gastric t-cell lymphoma in one free-ranging giant armadillo (priodontes maximus) from brazilian pantanal infected by a novel gammaherpesvirus, proposed as cingulatid gammaherpesvirus 1 (cihv-1). by chromogenic in situ hybridisation against epstein-barr virus some neop ...202134132049
neutralizing antibodies against epstein-barr virus infection of b cells can protect from oral viral challenge in the rhesus macaque animal model.epstein-barr virus (ebv) and related lymphocryptoviruses (lcvs) from nonhuman primates are transmitted through oral secretions, penetrate the mucosal epithelium, and establish persistent infection in b cells. to determine whether neutralizing antibodies against epithelial or b cell infection could block oral transmission and persistent lcv infection, we use rhesus macaques, the most accurate animal model for ebv infection by faithfully reproducing acute and persistent infection in humans. naive ...202134337567
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